Restriction map for
pF1KA0335
(Circular) (Six-base) MAPSORT of: pF1KA0335.seq Check: 909 from: 1 to: 7562
pF1KA0335 7562 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 4600 4600
Size: 7562
Acc65I G'GTAC_C
Cuts at: 1882 4578 1882
Size: 2696 4866
AclI AA'CG_TT
Cuts at: 7402 7402
Size: 7562
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 443 838 1202 1340 2452 2471 3536 4043 5387
Size: 395 364 138 1112 19 1065 507 1344
Cuts at: 5387 5819 6172 6834 443
Size: 432 353 662 1171
Fragments arranged by size:
1344 1171 1112 1065 662 507 432 395
364 353 138 19
AflII C'TTAA_G
Cuts at: 1039 1150 2770 3460 4231 1039
Size: 111 1620 690 771 4370
Fragments arranged by size:
4370 1620 771 690 111
AflIII A'CryG_T
Cuts at: 945 4224 4547 4914 6714 6995 945
Size: 3279 323 367 1800 281 1512
Fragments arranged by size:
3279 1800 1512 367 323 281
AleI CACnn'nnGTG
Cuts at: 1144 1144
Size: 7562
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1668 1700 1668
Size: 32 7530
AlwNI CAG_nnn'CTG
Cuts at: 1534 3785 3935 4867 6305 1534
Size: 2251 150 932 1438 2791
Fragments arranged by size:
2791 2251 1438 932 150
ApaLI G'TGCA_C
Cuts at: 6400 6400
Size: 7562
ApoI r'AATT_y
Cuts at: 215 800 1800 1813 2325 2437 2673 2680 2747
Size: 585 1000 13 512 112 236 7 67
Cuts at: 2747 2838 2874 3545 3866 4213 4450 4566 6915
Size: 91 36 671 321 347 237 116 2349
Cuts at: 6915 7171 215
Size: 256 606
Fragments arranged by size:
2349 1000 671 606 585 512 347 321
256 237 236 116 112 91 67 36
13 7
AseI AT'TA_AT
Cuts at: 20 1967 2651 20
Size: 1947 684 4931
Fragments arranged by size:
4931 1947 684
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7562
AvaI C'yCGr_G
Cuts at: 1870 4535 4582 1870
Size: 2665 47 4850
Fragments arranged by size:
4850 2665 47
AvrII C'CTAG_G
Cuts at: 4057 4129 4057
Size: 72 7490
BamHI G'GATC_C
Cuts at: 4587 4587
Size: 7562
BanI G'GyrC_C
Cuts at: 201 533 916 1488 1882 3097 4578 4992 5240
Size: 332 383 572 394 1215 1481 414 248
Cuts at: 5240 5275 201
Size: 35 2488
Fragments arranged by size:
2488 1481 1215 572 414 394 383 332
248 35
BanII G_rGCy'C
Cuts at: 2179 4576 5606 2179
Size: 2397 1030 4135
Fragments arranged by size:
4135 2397 1030
BbeI G_GCGC'C
Cuts at: 4996 5244 4996
Size: 248 7314
BbsI GAAGACnn'nnnn_
Cuts at: 1087 1190 1360 2366 4426 1087
Size: 103 170 1006 2060 4223
Fragments arranged by size:
4223 2060 1006 170 103
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 7528
BciVI GTATCCnnnnn_n'
Cuts at: 3013 4485 5460 6516 7562 3013
Size: 1472 975 1056 1046 3013
Fragments arranged by size:
3013 1472 1056 1046 975
BclI T'GATC_A
Cuts at: 5082 5082
Size: 7562
BfrBI ATG'CAT
Cuts at: 1933 1953 1933
Size: 20 7542
BglII A'GATC_T
Cuts at: 5077 7114 5077
Size: 2037 5525
BlpI GC'TnA_GC
Cuts at: 4674 4674
Size: 7562
Bme1580I G_kGCm'C
Cuts at: 3102 5187 5280 6404 3102
Size: 2085 93 1124 4260
Fragments arranged by size:
4260 2085 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1310 2620 4774 4855 5190 7334 1310
Size: 1310 2154 81 335 2144 1538
Fragments arranged by size:
2154 2144 1538 1310 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5929 5929
Size: 7562
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2401 2401
Size: 7562
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1127 4738 5617 6331 6629 1127
Size: 3611 879 714 298 2060
Fragments arranged by size:
3611 2060 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 7266 7266
Size: 7562
BsaAI yAC'GTr
Cuts at: 4915 4915
Size: 7562
BsaBI GATnn'nnATC
Cuts at: 6 5076 7113 6
Size: 5070 2037 455
Fragments arranged by size:
5070 2037 455
BsaHI Gr'CG_yC
Cuts at: 4993 5241 5943 7439 4993
Size: 248 702 1496 5116
Fragments arranged by size:
5116 1496 702 248
BsaWI w'CCGG_w
Cuts at: 4751 5272 5950 6361 6508 6782 4751
Size: 521 678 411 147 274 5531
Fragments arranged by size:
5531 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 403 1280 3665 403
Size: 877 2385 4300
Fragments arranged by size:
4300 2385 877
BseYI C'CCAG_C
Cuts at: 3102 4987 5745 6410 6818 3102
Size: 1885 758 665 408 3846
Fragments arranged by size:
3846 1885 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 5104 5150 6380 104
Size: 5000 46 1230 1286
Fragments arranged by size:
5000 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2179 3328 4576 5354 5544 6404 2179
Size: 1149 1248 778 190 860 3337
Fragments arranged by size:
3337 1248 1149 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 2179 3102 3328 4576 5187 5280 5354 5544 5606
Size: 923 226 1248 611 93 74 190 62
Cuts at: 5606 6404 2179
Size: 798 3337
Fragments arranged by size:
3337 1248 923 798 611 226 190 93
74 62
BspHI T'CATG_A
Cuts at: 1349 5994 1349
Size: 4645 2917
BspMI ACCTGCnnnn'nnnn_
Cuts at: 4612 4826 5128 5509 4612
Size: 214 302 381 6665
Fragments arranged by size:
6665 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5854 5908 7392 7557 50
Size: 5804 54 1484 165 55
Fragments arranged by size:
5804 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 397 3610 3988 5474 397
Size: 3213 378 1486 2485
Fragments arranged by size:
3213 2485 1486 378
BsrFI r'CCGG_y
Cuts at: 5560 5741 5560
Size: 181 7381
BsrGI T'GTAC_A
Cuts at: 394 2306 394
Size: 1912 5650
BssSI C'ACGA_G
Cuts at: 2566 3107 5833 6541 2566
Size: 541 2726 708 3587
Fragments arranged by size:
3587 2726 708 541
BstAPI GCAn_nnn'nTGC
Cuts at: 4939 4939
Size: 7562
BstBI TT'CG_AA
Cuts at: 2596 5923 2596
Size: 3327 4235
BstXI CCAn_nnnn'nTGG
Cuts at: 763 763
Size: 7562
BstYI r'GATC_y
Cuts at: 1 4587 5054 5077 5412 5658 5964 5976 6062
Size: 4586 467 23 335 246 306 12 86
Cuts at: 6062 7114 1
Size: 1052 449
Fragments arranged by size:
4586 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 537 537
Size: 7562
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5512 5693 5856 5512
Size: 181 163 7218
Fragments arranged by size:
7218 181 163
BtsI GCAGTG_nn'
Cuts at: 2087 2646 4805 2087
Size: 559 2159 4844
Fragments arranged by size:
4844 2159 559
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1797 1832 1797
Size: 35 7527
DraI TTT'AAA
Cuts at: 158 473 603 2216 2237 2873 3428 3563 4338
Size: 315 130 1613 21 636 555 135 775
Cuts at: 4338 4561 158
Size: 223 3159
Fragments arranged by size:
3159 1613 775 636 555 315 223 135
130 21
DraIII CAC_nnn'GTG
Cuts at: 712 712
Size: 7562
DrdI GACnn_nn'nnGTC
Cuts at: 5268 6612 5268
Size: 1344 6218
EaeI y'GGCC_r
Cuts at: 5147 5321 5712 5739 7084 5147
Size: 174 391 27 1345 5625
Fragments arranged by size:
5625 1345 391 174 27
EagI C'GGCC_G
Cuts at: 5147 5147
Size: 7562
EarI CTCTTCn'nnn_
Cuts at: 422 1316 5585 5795 422
Size: 894 4269 210 2189
Fragments arranged by size:
4269 2189 894 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5770 6512 6658 7372 5770
Size: 742 146 714 5960
Fragments arranged by size:
5960 742 714 146
EcoICRI GAG'CTC
Cuts at: 2177 4574 2177
Size: 2397 5165
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 443 838 1202 1340 2401 2452 2471 3536 4043
Size: 395 364 138 1061 51 19 1065 507
Cuts at: 4043 5387 5819 6172 6834 443
Size: 1344 432 353 662 1171
Fragments arranged by size:
1344 1171 1065 1061 662 507 432 395
364 353 138 51 19
EcoO109I rG'GnC_Cy
Cuts at: 1369 3253 4701 1369
Size: 1884 1448 4230
Fragments arranged by size:
4230 1884 1448
EcoRI G'AATT_C
Cuts at: 4566 4566
Size: 7562
EcoRV GAT'ATC
Cuts at: 1709 1709
Size: 7562
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 868 1775 2123 3310 5581 868
Size: 907 348 1187 2271 2849
Fragments arranged by size:
2849 2271 1187 907 348
FspI TGC'GCA
Cuts at: 5343 5343
Size: 7562
HaeII r_GCGC'y
Cuts at: 4996 5244 6474 7245 4996
Size: 248 1230 771 5313
Fragments arranged by size:
5313 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3655 3687 5404 5434 5436 5466 3655
Size: 32 1717 30 2 30 5751
Fragments arranged by size:
5751 1717 32 30 30 2
HincII GTy'rAC
Cuts at: 309 1836 1911 4601 309
Size: 1527 75 2690 3270
Fragments arranged by size:
3270 2690 1527 75
KasI G'GCGC_C
Cuts at: 4992 5240 4992
Size: 248 7314
KpnI G_GTAC'C
Cuts at: 1886 4582 1886
Size: 2696 4866
MfeI C'AATT_G
Cuts at: 2461 2461
Size: 7562
MluI A'CGCG_T
Cuts at: 6995 6995
Size: 7562
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2027 2154 6321 6505 2027
Size: 127 4167 184 3084
Fragments arranged by size:
4167 3084 184 127
MscI TGG'CCA
Cuts at: 5323 5323
Size: 7562
MslI CAynn'nnrTG
Cuts at: 974 1144 1214 1650 5551 5678 974
Size: 170 70 436 3901 127 2858
Fragments arranged by size:
3901 2858 436 170 127 70
MspA1I CmG'CkG
Cuts at: 467 3082 4673 4987 5347 6131 6376 467
Size: 2615 1591 314 360 784 245 1653
Fragments arranged by size:
2615 1653 1591 784 360 314 245
NaeI GCC'GGC
Cuts at: 5743 5743
Size: 7562
NarI GG'CG_CC
Cuts at: 4993 5241 4993
Size: 248 7314
NcoI C'CATG_G
Cuts at: 537 537
Size: 7562
NgoMIV G'CCGG_C
Cuts at: 5741 5741
Size: 7562
NsiI A_TGCA'T
Cuts at: 1935 1955 1935
Size: 20 7542
NspI r_CATG'y
Cuts at: 949 3309 3749 4228 4551 4615 5115 6718 949
Size: 2360 440 479 323 64 500 1603 1793
Fragments arranged by size:
2360 1793 1603 500 479 440 323 64
PciI A'CATG_T
Cuts at: 945 4224 4547 6714 945
Size: 3279 323 2167 1793
Fragments arranged by size:
3279 2167 1793 323
PflMI CCAn_nnn'nTGG
Cuts at: 1572 3109 1572
Size: 1537 6025
PfoI T'CCnGG_A
Cuts at: 2967 2967
Size: 7562
PmeI GTTT'AAAC
Cuts at: 4561 4561
Size: 7562
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6008 6040 6008
Size: 32 7530
PpuMI rG'GwC_Cy
Cuts at: 1369 1369
Size: 7562
PsiI TTA'TAA
Cuts at: 809 952 4286 809
Size: 143 3334 4085
Fragments arranged by size:
4085 3334 143
PstI C_TGCA'G
Cuts at: 1035 4609 5294 1035
Size: 3574 685 3303
Fragments arranged by size:
3574 3303 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7562
PvuII CAG'CTG
Cuts at: 467 4987 5347 467
Size: 4520 360 2682
Fragments arranged by size:
4520 2682 360
RsrII CG'GwC_CG
Cuts at: 5757 5757
Size: 7562
SacI G_AGCT'C
Cuts at: 2179 4576 2179
Size: 2397 5165
SalI G'TCGA_C
Cuts at: 4599 4599
Size: 7562
SapI GCTCTTCn'nnn_
Cuts at: 5585 5795 5585
Size: 210 7352
SbfI CC_TGCA'GG
Cuts at: 4609 4609
Size: 7562
ScaI AGT'ACT
Cuts at: 3486 7079 3486
Size: 3593 3969
SfcI C'TryA_G
Cuts at: 33 1031 4605 5290 6258 6449 33
Size: 998 3574 685 968 191 1146
Fragments arranged by size:
3574 1146 998 968 685 191
SfoI GGC'GCC
Cuts at: 4994 5242 4994
Size: 248 7314
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7562
SmaI CCC'GGG
Cuts at: 4584 4584
Size: 7562
SmlI C'TyrA_G
Cuts at: 1039 1142 1150 2770 3460 4231 4717 5632 6346
Size: 103 8 1620 690 771 486 915 714
Cuts at: 6346 6608 1039
Size: 262 1993
Fragments arranged by size:
1993 1620 915 771 714 690 486 262
103 8
SpeI A'CTAG_T
Cuts at: 67 2160 67
Size: 2093 5469
SphI G_CATG'C
Cuts at: 4615 5115 4615
Size: 500 7062
SspI AAT'ATT
Cuts at: 276 904 3536 4437 276
Size: 628 2632 901 3401
Fragments arranged by size:
3401 2632 901 628
StyI C'CwwG_G
Cuts at: 537 2007 4057 4129 4696 5050 537
Size: 1470 2050 72 567 354 3049
Fragments arranged by size:
3049 2050 1470 567 354 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 769 5145 5278 5737 5946 769
Size: 4376 133 459 209 2385
Fragments arranged by size:
4376 2385 459 209 133
TatI w'GTAC_w
Cuts at: 394 1362 2306 3484 4227 7077 394
Size: 968 944 1178 743 2850 879
Fragments arranged by size:
2850 1178 968 944 879 743
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2066 2098 2987 3019 7079 7111 2066
Size: 32 889 32 4060 32 2517
Fragments arranged by size:
4060 2517 889 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2778 5359 2778
Size: 2581 4981
XbaI T'CTAG_A
Cuts at: 3673 3922 4593 3673
Size: 249 671 6642
Fragments arranged by size:
6642 671 249
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5057 5057
Size: 7562
XmaI C'CCGG_G
Cuts at: 4582 4582
Size: 7562
XmnI GAAnn'nnTTC
Cuts at: 1084 2602 1084
Size: 1518 6044
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI
PflMI PfoI PmeI PpiI PpuMI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AlfI ApaI AscI
BaeI BbvCI BglI BmgBI BmtI Bpu10I BsaXI BsgI
BsiWI BsmI BsmBI BspEI BssHII BstEII BstZ17I Bsu36I
ClaI EcoNI FseI FspAI HindIII HpaI NdeI NheI
NotI NruI PacI PasI PmlI PshAI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI ZraI