Restriction map for
pF1KA0338
(Circular) (Six-base) MAPSORT of: pF1KA0338.seq Check: 560 from: 1 to: 5756
pF1KA0338 5756 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 962 2696 2794 962
Size: 1734 98 3924
Fragments arranged by size:
3924 1734 98
Acc65I G'GTAC_C
Cuts at: 2772 2772
Size: 5756
AclI AA'CG_TT
Cuts at: 5596 5596
Size: 5756
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1642 1681 1909 3581 4013 4760 5028 1642
Size: 39 228 1672 432 747 268 2370
Fragments arranged by size:
2370 1672 747 432 268 228 39
AflIII A'CryG_T
Cuts at: 3108 4212 5189 3108
Size: 1104 977 3675
Fragments arranged by size:
3675 1104 977
AgeI A'CCGG_T
Cuts at: 1178 4144 1178
Size: 2966 2790
AleI CACnn'nnGTG
Cuts at: 199 199
Size: 5756
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1867 1899 1867
Size: 32 5724
AlwNI CAG_nnn'CTG
Cuts at: 471 1456 1510 2631 3061 4628 471
Size: 985 54 1121 430 1567 1599
Fragments arranged by size:
1599 1567 1121 985 430 54
ApaI G_GGCC'C
Cuts at: 1833 2103 1833
Size: 270 5486
ApaLI G'TGCA_C
Cuts at: 4526 4526
Size: 5756
ApoI r'AATT_y
Cuts at: 609 2760 5109 5365 609
Size: 2151 2349 256 1000
Fragments arranged by size:
2349 2151 1000 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5756
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5756
AvaI C'yCGr_G
Cuts at: 172 848 903 1826 2081 2776 172
Size: 676 55 923 255 695 3152
Fragments arranged by size:
3152 923 695 676 255 55
BamHI G'GATC_C
Cuts at: 2781 2781
Size: 5756
BanI G'GyrC_C
Cuts at: 1753 2497 2772 3186 3434 3469 1753
Size: 744 275 414 248 35 4040
Fragments arranged by size:
4040 744 414 275 248 35
BanII G_rGCy'C
Cuts at: 757 827 1250 1279 1649 1670 1798 1833 2036
Size: 70 423 29 370 21 128 35 203
Cuts at: 2036 2103 2189 2770 3800 757
Size: 67 86 581 1030 2713
Fragments arranged by size:
2713 1030 581 423 370 203 128 86
70 67 35 29 21
BbeI G_GCGC'C
Cuts at: 2501 3190 3438 2501
Size: 689 248 4819
Fragments arranged by size:
4819 689 248
BbsI GAAGACnn'nnnn_
Cuts at: 668 2015 668
Size: 1347 4409
BbvCI CC'TCA_GC
Cuts at: 794 1511 1786 1961 794
Size: 717 275 175 4589
Fragments arranged by size:
4589 717 275 175
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 163 197 1986 2020 2138 2172 163
Size: 34 1789 34 118 34 3747
Fragments arranged by size:
3747 1789 118 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3654 4415 5756 3654
Size: 761 1341 3654
Fragments arranged by size:
3654 1341 761
BclI T'GATC_A
Cuts at: 3276 3276
Size: 5756
BglII A'GATC_T
Cuts at: 1085 3271 5308 1085
Size: 2186 2037 1533
Fragments arranged by size:
2186 2037 1533
BlpI GC'TnA_GC
Cuts at: 773 2868 773
Size: 2095 3661
Bme1580I G_kGCm'C
Cuts at: 1756 1833 2103 3381 3474 4530 1756
Size: 77 270 1278 93 1056 2982
Fragments arranged by size:
2982 1278 1056 270 93 77
BmgBI CAC'GTC
Cuts at: 1822 1822
Size: 5756
BmrI ACTGGGnnnn_n'
Cuts at: 760 1710 2172 2609 2968 3049 3384 5528 760
Size: 950 462 437 359 81 335 2144 988
Fragments arranged by size:
2144 988 950 462 437 359 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 252 4123 252
Size: 3871 1885
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 877 1908 1975 877
Size: 1031 67 4658
Fragments arranged by size:
4658 1031 67
Bpu10I CC'TnA_GC
Cuts at: 149 794 1244 1511 1786 1961 2030 149
Size: 645 450 267 275 175 69 3875
Fragments arranged by size:
3875 645 450 275 267 175 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1066 2371 2932 3811 4303 4601 1066
Size: 1305 561 879 492 298 2221
Fragments arranged by size:
2221 1305 879 561 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2350 5460 2350
Size: 3110 2646
BsaAI yAC'GTr
Cuts at: 3109 3109
Size: 5756
BsaBI GATnn'nnATC
Cuts at: 6 2615 3270 5307 6
Size: 2609 655 2037 455
Fragments arranged by size:
2609 2037 655 455
BsaHI Gr'CG_yC
Cuts at: 2498 3187 3435 4137 5633 2498
Size: 689 248 702 1496 2621
Fragments arranged by size:
2621 1496 702 689 248
BsaWI w'CCGG_w
Cuts at: 593 1178 1286 2945 3466 4144 4418 4565 4976
Size: 585 108 1659 521 678 274 147 411
Cuts at: 4976 593
Size: 1373
Fragments arranged by size:
1659 1373 678 585 521 411 274 147
108
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 421 451 421
Size: 30 5726
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 169 313 811 874 1113 1528 1716 1816 2737
Size: 144 498 63 239 415 188 100 921
Cuts at: 2737 169
Size: 3188
Fragments arranged by size:
3188 921 498 415 239 188 144 100
63
BseYI C'CCAG_C
Cuts at: 257 346 650 777 1422 2104 2288 3181 3939
Size: 89 304 127 645 682 184 893 758
Cuts at: 3939 4516 5012 257
Size: 577 496 1001
Fragments arranged by size:
1001 893 758 682 645 577 496 304
184 127 89
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 787 952 1331 787
Size: 165 379 5212
Fragments arranged by size:
5212 379 165
BsiEI CG_ry'CG
Cuts at: 104 2040 3298 3344 4552 104
Size: 1936 1258 46 1208 1308
Fragments arranged by size:
1936 1308 1258 1208 46
BsiHKAI G_wGCw'C
Cuts at: 827 1153 1649 1924 2036 2770 3548 3738 4530
Size: 326 496 275 112 734 778 190 792
Cuts at: 4530 827
Size: 2053
Fragments arranged by size:
2053 792 778 734 496 326 275 190
112
BsmI GAATG_Cn'
Cuts at: 939 939
Size: 5756
Bsp1286I G_dGCh'C
Cuts at: 757 827 1153 1250 1279 1649 1670 1756 1798
Size: 70 326 97 29 370 21 86 42
Cuts at: 1798 1833 1924 2036 2103 2189 2770 3381 3474
Size: 35 91 112 67 86 581 611 93
Cuts at: 3474 3548 3738 3800 4530 757
Size: 74 190 62 730 1983
Fragments arranged by size:
1983 730 611 581 370 326 190 112
97 93 91 86 86 74 70 67
62 42 35 29 21
BspEI T'CCGG_A
Cuts at: 593 4976 593
Size: 4383 1373
BspHI T'CATG_A
Cuts at: 4932 4932
Size: 5756
BspMI ACCTGCnnnn'nnnn_
Cuts at: 974 2283 2806 3020 3322 3703 974
Size: 1309 523 214 302 381 3027
Fragments arranged by size:
3027 1309 523 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4048 4102 5586 5751 50
Size: 3998 54 1484 165 55
Fragments arranged by size:
3998 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3668 3668
Size: 5756
BsrFI r'CCGG_y
Cuts at: 716 1043 1178 1340 3754 3935 4144 716
Size: 327 135 162 2414 181 209 2328
Fragments arranged by size:
2414 2328 327 209 181 162 135
BssSI C'ACGA_G
Cuts at: 4027 4385 4027
Size: 358 5398
BstAPI GCAn_nnn'nTGC
Cuts at: 3133 3133
Size: 5756
BstBI TT'CG_AA
Cuts at: 2006 4117 2006
Size: 2111 3645
BstEII G'GTnAC_C
Cuts at: 2679 2679
Size: 5756
BstYI r'GATC_y
Cuts at: 1 590 1076 1085 1118 2085 2781 3248 3271
Size: 589 486 9 33 967 696 467 23
Cuts at: 3271 3606 3852 4864 4950 4962 5308 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 967 696 589 486 467 449 346
335 246 86 33 23 12 9
BstZ17I GTA'TAC
Cuts at: 2697 2697
Size: 5756
BtgI C'CryG_G
Cuts at: 216 1992 2351 2372 2438 216
Size: 1776 359 21 66 3534
Fragments arranged by size:
3534 1776 359 66 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1865 3706 3887 4050 1865
Size: 1841 181 163 3571
Fragments arranged by size:
3571 1841 181 163
BtsI GCAGTG_nn'
Cuts at: 2501 2999 2501
Size: 498 5258
DraI TTT'AAA
Cuts at: 2755 2755
Size: 5756
DrdI GACnn_nn'nnGTC
Cuts at: 1975 3462 4320 1975
Size: 1487 858 3411
Fragments arranged by size:
3411 1487 858
EaeI y'GGCC_r
Cuts at: 219 456 1904 2222 2435 2639 3341 3515 3906
Size: 237 1448 318 213 204 702 174 391
Cuts at: 3906 3933 5278 219
Size: 27 1345 697
Fragments arranged by size:
1448 1345 702 697 391 318 237 213
204 174 27
EagI C'GGCC_G
Cuts at: 3341 3341
Size: 5756
EarI CTCTTCn'nnn_
Cuts at: 3779 3989 3779
Size: 210 5546
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3964 4274 4420 5566 3964
Size: 310 146 1146 4154
Fragments arranged by size:
4154 1146 310 146
EcoICRI GAG'CTC
Cuts at: 825 1647 2034 2768 825
Size: 822 387 734 3813
Fragments arranged by size:
3813 822 734 387
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 877 1642 1681 1908 1909 1975 3581 4013 4760
Size: 765 39 227 1 66 1606 432 747
Cuts at: 4760 5028 877
Size: 268 1605
Fragments arranged by size:
1606 1605 765 747 432 268 227 66
39 1
EcoNI CCTnn'n_nnAGG
Cuts at: 508 1095 508
Size: 587 5169
EcoO109I rG'GnC_Cy
Cuts at: 124 159 1301 1453 1655 1830 2067 2099 2100
Size: 35 1142 152 202 175 237 32 1
Cuts at: 2100 2153 2628 2895 124
Size: 53 475 267 2985
Fragments arranged by size:
2985 1142 475 267 237 202 175 152
53 35 32 1
EcoRI G'AATT_C
Cuts at: 2760 2760
Size: 5756
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 534 3775 534
Size: 3241 2515
FspI TGC'GCA
Cuts at: 3537 3537
Size: 5756
HaeII r_GCGC'y
Cuts at: 1331 2003 2501 3190 3438 4460 5439 1331
Size: 672 498 689 248 1022 979 1648
Fragments arranged by size:
1648 1022 979 689 672 498 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1471 1503 2104 2136 2347 2379 3598 3628 3630
Size: 32 601 32 211 32 1219 30 2
Cuts at: 3630 3660 1471
Size: 30 3567
Fragments arranged by size:
3567 1219 601 211 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2795 2795
Size: 5756
HindIII A'AGCT_T
Cuts at: 943 943
Size: 5756
KasI G'GCGC_C
Cuts at: 2497 3186 3434 2497
Size: 689 248 4819
Fragments arranged by size:
4819 689 248
KpnI G_GTAC'C
Cuts at: 2776 2776
Size: 5756
MfeI C'AATT_G
Cuts at: 1154 1154
Size: 5756
MluI A'CGCG_T
Cuts at: 5189 5189
Size: 5756
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1558 4427 4611 1558
Size: 2869 184 2703
Fragments arranged by size:
2869 2703 184
MscI TGG'CCA
Cuts at: 1906 2224 2437 2641 3517 1906
Size: 318 213 204 876 4145
Fragments arranged by size:
4145 876 318 213 204
MslI CAynn'nnrTG
Cuts at: 199 3745 3872 199
Size: 3546 127 2083
Fragments arranged by size:
3546 2083 127
MspA1I CmG'CkG
Cuts at: 187 654 693 2407 2867 3181 3541 4554 4799
Size: 467 39 1714 460 314 360 1013 245
Cuts at: 4799 187
Size: 1144
Fragments arranged by size:
1714 1144 1013 467 460 360 314 245
39
NaeI GCC'GGC
Cuts at: 718 3937 718
Size: 3219 2537
NarI GG'CG_CC
Cuts at: 2498 3187 3435 2498
Size: 689 248 4819
Fragments arranged by size:
4819 689 248
NcoI C'CATG_G
Cuts at: 1992 2372 1992
Size: 380 5376
NgoMIV G'CCGG_C
Cuts at: 716 3935 716
Size: 3219 2537
NspI r_CATG'y
Cuts at: 745 805 2809 3309 4216 745
Size: 60 2004 500 907 2285
Fragments arranged by size:
2285 2004 907 500 60
PciI A'CATG_T
Cuts at: 4212 4212
Size: 5756
PflMI CCAn_nnn'nTGG
Cuts at: 2631 2631
Size: 5756
PfoI T'CCnGG_A
Cuts at: 2392 2392
Size: 5756
PmeI GTTT'AAAC
Cuts at: 2755 2755
Size: 5756
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4895 4927 4895
Size: 32 5724
PpuMI rG'GwC_Cy
Cuts at: 1301 1655 1301
Size: 354 5402
PshAI GACnn'nnGTC
Cuts at: 2266 2266
Size: 5756
PspOMI G'GGCC_C
Cuts at: 1829 2099 1829
Size: 270 5486
PstI C_TGCA'G
Cuts at: 213 692 2261 2663 2803 3488 213
Size: 479 1569 402 140 685 2481
Fragments arranged by size:
2481 1569 685 479 402 140
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5756
PvuII CAG'CTG
Cuts at: 654 693 2407 3181 3541 654
Size: 39 1714 774 360 2869
Fragments arranged by size:
2869 1714 774 360 39
RsrII CG'GwC_CG
Cuts at: 3951 3951
Size: 5756
SacI G_AGCT'C
Cuts at: 827 1649 2036 2770 827
Size: 822 387 734 3813
Fragments arranged by size:
3813 822 734 387
SalI G'TCGA_C
Cuts at: 2793 2793
Size: 5756
SapI GCTCTTCn'nnn_
Cuts at: 3779 3989 3779
Size: 210 5546
SbfI CC_TGCA'GG
Cuts at: 213 2803 213
Size: 2590 3166
ScaI AGT'ACT
Cuts at: 5273 5273
Size: 5756
SexAI A'CCwGG_T
Cuts at: 479 479
Size: 5756
SfcI C'TryA_G
Cuts at: 33 209 309 688 2257 2659 2799 3484 4477
Size: 176 100 379 1569 402 140 685 993
Cuts at: 4477 4668 33
Size: 191 1121
Fragments arranged by size:
1569 1121 993 685 402 379 191 176
140 100
SfoI GGC'GCC
Cuts at: 2499 3188 3436 2499
Size: 689 248 4819
Fragments arranged by size:
4819 689 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5756
SmaI CCC'GGG
Cuts at: 850 905 1828 2083 2778 850
Size: 55 923 255 695 3828
Fragments arranged by size:
3828 923 695 255 55
SmlI C'TyrA_G
Cuts at: 1081 2386 2911 3826 4318 4580 1081
Size: 1305 525 915 492 262 2257
Fragments arranged by size:
2257 1305 915 525 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5756
SphI G_CATG'C
Cuts at: 745 2809 3309 745
Size: 2064 500 3192
Fragments arranged by size:
3192 2064 500
StyI C'CwwG_G
Cuts at: 572 977 1256 1748 1992 2372 2890 3244 572
Size: 405 279 492 244 380 518 354 3084
Fragments arranged by size:
3084 518 492 405 380 354 279 244
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 239 769 1269 1419 2302 2548 3339 3472 3931
Size: 530 500 150 883 246 791 133 459
Cuts at: 3931 4140 239
Size: 209 1855
Fragments arranged by size:
1855 883 791 530 500 459 246 209
150 133
TatI w'GTAC_w
Cuts at: 5271 5271
Size: 5756
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 421 453 5273 5305 421
Size: 32 4820 32 872
Fragments arranged by size:
4820 872 32 32
Tth111I GACn'n_nGTC
Cuts at: 481 3553 481
Size: 3072 2684
XbaI T'CTAG_A
Cuts at: 2787 2787
Size: 5756
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1263 2296 3251 1263
Size: 1033 955 3768
Fragments arranged by size:
3768 1033 955
XmaI C'CCGG_G
Cuts at: 848 903 1826 2081 2776 848
Size: 55 923 255 695 3828
Fragments arranged by size:
3828 923 695 255 55
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI BstZ17I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AscI AvrII
BaeI BfrBI BglI BmtI BsiWI BsmBI BsrGI BssHII
BstXI Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HpaI NdeI NheI NotI NruI NsiI PacI PasI
PmlI PsiI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI