Restriction map for
pF1KA0343
(Circular) (Six-base) MAPSORT of: pF1KA0343.seq Check: 6065 from: 1 to: 6653
pF1KA0343 6653 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1434 3207 1434
Size: 1773 4880
AatII G_ACGT'C
Cuts at: 2742 2742
Size: 6653
AccI GT'mk_AC
Cuts at: 3691 3691
Size: 6653
Acc65I G'GTAC_C
Cuts at: 3669 3669
Size: 6653
AclI AA'CG_TT
Cuts at: 6493 6493
Size: 6653
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1816 2165 2374 3443 3611 4478 4910 5657 5925
Size: 349 209 1069 168 867 432 747 268
Cuts at: 5925 1816
Size: 2544
Fragments arranged by size:
2544 1069 867 747 432 349 268 209
168
AfeI AGC'GCT
Cuts at: 139 1935 139
Size: 1796 4857
AflIII A'CryG_T
Cuts at: 1621 1892 1894 4005 5109 6086 1621
Size: 271 2 2111 1104 977 2188
Fragments arranged by size:
2188 2111 1104 977 271 2
AgeI A'CCGG_T
Cuts at: 5041 5041
Size: 6653
AleI CACnn'nnGTG
Cuts at: 543 921 1897 2305 543
Size: 378 976 408 4891
Fragments arranged by size:
4891 976 408 378
AlwNI CAG_nnn'CTG
Cuts at: 1054 2329 2520 3958 5525 1054
Size: 1275 191 1438 1567 2182
Fragments arranged by size:
2182 1567 1438 1275 191
ApaI G_GGCC'C
Cuts at: 2863 2863
Size: 6653
ApaLI G'TGCA_C
Cuts at: 191 2641 5423 191
Size: 2450 2782 1421
Fragments arranged by size:
2782 2450 1421
ApoI r'AATT_y
Cuts at: 1583 2086 3065 3099 3105 3636 3657 6006 6262
Size: 503 979 34 6 531 21 2349 256
Cuts at: 6262 1583
Size: 1974
Fragments arranged by size:
2349 1974 979 531 503 256 34 21
6
AseI AT'TA_AT
Cuts at: 20 20
Size: 6653
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6653
AvaI C'yCGr_G
Cuts at: 287 2542 3673 287
Size: 2255 1131 3267
Fragments arranged by size:
3267 2255 1131
BamHI G'GATC_C
Cuts at: 2967 3678 2967
Size: 711 5942
BanI G'GyrC_C
Cuts at: 2132 2408 3612 3669 4083 4331 4366 2132
Size: 276 1204 57 414 248 35 4419
Fragments arranged by size:
4419 1204 414 276 248 57 35
BanII G_rGCy'C
Cuts at: 2810 2863 3667 4697 2810
Size: 53 804 1030 4766
Fragments arranged by size:
4766 1030 804 53
BbeI G_GCGC'C
Cuts at: 2412 4087 4335 2412
Size: 1675 248 4730
Fragments arranged by size:
4730 1675 248
BbsI GAAGACnn'nnnn_
Cuts at: 233 755 2582 2716 3428 233
Size: 522 1827 134 712 3458
Fragments arranged by size:
3458 1827 712 522 134
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 6619
BciVI GTATCCnnnnn_n'
Cuts at: 2469 4551 5312 6653 2469
Size: 2082 761 1341 2469
Fragments arranged by size:
2469 2082 1341 761
BclI T'GATC_A
Cuts at: 1764 2504 4173 1764
Size: 740 1669 4244
Fragments arranged by size:
4244 1669 740
BfrBI ATG'CAT
Cuts at: 1084 1084
Size: 6653
BglII A'GATC_T
Cuts at: 4168 6205 4168
Size: 2037 4616
BlpI GC'TnA_GC
Cuts at: 1403 1942 3765 1403
Size: 539 1823 4291
Fragments arranged by size:
4291 1823 539
Bme1580I G_kGCm'C
Cuts at: 161 195 2645 2863 4278 4371 5427 161
Size: 34 2450 218 1415 93 1056 1387
Fragments arranged by size:
2450 1415 1387 1056 218 93 34
BmrI ACTGGGnnnn_n'
Cuts at: 969 1811 2326 2647 2655 2900 3865 3946 4281
Size: 842 515 321 8 245 965 81 335
Cuts at: 4281 6425 969
Size: 2144 1197
Fragments arranged by size:
2144 1197 965 842 515 335 321 245
81 8
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 707 2596 3561 5020 707
Size: 1889 965 1459 2340
Fragments arranged by size:
2340 1889 1459 965
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 851 940 1952 2591 2876 851
Size: 89 1012 639 285 4628
Fragments arranged by size:
4628 1012 639 285 89
Bpu10I CC'TnA_GC
Cuts at: 2631 2631
Size: 6653
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 677 3829 4708 5200 5498 677
Size: 3152 879 492 298 1832
Fragments arranged by size:
3152 1832 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 445 1496 6357 445
Size: 1051 4861 741
Fragments arranged by size:
4861 1051 741
BsaAI yAC'GTr
Cuts at: 1622 1895 2193 2348 4006 1622
Size: 273 298 155 1658 4269
Fragments arranged by size:
4269 1658 298 273 155
BsaBI GATnn'nnATC
Cuts at: 6 4167 6204 6
Size: 4161 2037 455
Fragments arranged by size:
4161 2037 455
BsaHI Gr'CG_yC
Cuts at: 2254 2409 2739 4084 4332 5034 6530 2254
Size: 155 330 1345 248 702 1496 2377
Fragments arranged by size:
2377 1496 1345 702 330 248 155
BsaWI w'CCGG_w
Cuts at: 3842 4363 5041 5315 5462 5873 3842
Size: 521 678 274 147 411 4622
Fragments arranged by size:
4622 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 488 518 1909 1939 1950 1956 1980 1986 3503
Size: 30 1391 30 11 6 24 6 1517
Cuts at: 3503 3533 488
Size: 30 3608
Fragments arranged by size:
3608 1517 1391 30 30 30 24 11
6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1484 1484
Size: 6653
BseYI C'CCAG_C
Cuts at: 1273 2171 2245 2862 4078 4836 5413 5909 1273
Size: 898 74 617 1216 758 577 496 2017
Fragments arranged by size:
2017 1216 898 758 617 577 496 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1352 1352
Size: 6653
BsiEI CG_ry'CG
Cuts at: 104 4195 4241 5449 104
Size: 4091 46 1208 1308
Fragments arranged by size:
4091 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 195 1539 2645 2913 3667 4445 4635 5427 195
Size: 1344 1106 268 754 778 190 792 1421
Fragments arranged by size:
1421 1344 1106 792 778 754 268 190
BsmI GAATG_Cn'
Cuts at: 983 1753 983
Size: 770 5883
BsmBI CGTCTCn'nnnn_
Cuts at: 1929 1929
Size: 6653
Bsp1286I G_dGCh'C
Cuts at: 161 195 1539 2645 2810 2863 2913 3667 4278
Size: 34 1344 1106 165 53 50 754 611
Cuts at: 4278 4371 4445 4635 4697 5427 161
Size: 93 74 190 62 730 1387
Fragments arranged by size:
1387 1344 1106 754 730 611 190 165
93 74 62 53 50 34
BspEI T'CCGG_A
Cuts at: 5873 5873
Size: 6653
BspHI T'CATG_A
Cuts at: 431 1542 5829 431
Size: 1111 4287 1255
Fragments arranged by size:
4287 1255 1111
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1434 3207 3703 3917 4219 4600 1434
Size: 1773 496 214 302 381 3487
Fragments arranged by size:
3487 1773 496 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4945 4999 6483 6648 50
Size: 4895 54 1484 165 55
Fragments arranged by size:
4895 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 882 926 1353 1448 2117 4565 882
Size: 44 427 95 669 2448 2970
Fragments arranged by size:
2970 2448 669 427 95 44
BsrFI r'CCGG_y
Cuts at: 3212 3585 4651 4832 5041 3212
Size: 373 1066 181 209 4824
Fragments arranged by size:
4824 1066 373 209 181
BsrGI T'GTAC_A
Cuts at: 475 475
Size: 6653
BssSI C'ACGA_G
Cuts at: 4924 5282 4924
Size: 358 6295
BstAPI GCAn_nnn'nTGC
Cuts at: 1081 4030 1081
Size: 2949 3704
BstBI TT'CG_AA
Cuts at: 2369 5014 2369
Size: 2645 4008
BstEII G'GTnAC_C
Cuts at: 390 390
Size: 6653
BstXI CCAn_nnnn'nTGG
Cuts at: 2129 2307 2419 2986 2129
Size: 178 112 567 5796
Fragments arranged by size:
5796 567 178 112
BstYI r'GATC_y
Cuts at: 1 2756 2967 3678 4145 4168 4503 4749 5761
Size: 2755 211 711 467 23 335 246 1012
Cuts at: 5761 5847 5859 6205 1
Size: 86 12 346 449
Fragments arranged by size:
2755 1012 711 467 449 346 335 246
211 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2075 3217 4603 4784 4947 2075
Size: 1142 1386 181 163 3781
Fragments arranged by size:
3781 1386 1142 181 163
BtsI GCAGTG_nn'
Cuts at: 2225 3896 2225
Size: 1671 4982
ClaI AT'CG_AT
Cuts at: 374 374
Size: 6653
DraI TTT'AAA
Cuts at: 1518 3095 3104 3652 1518
Size: 1577 9 548 4519
Fragments arranged by size:
4519 1577 548 9
DraIII CAC_nnn'GTG
Cuts at: 679 679
Size: 6653
DrdI GACnn_nn'nnGTC
Cuts at: 388 4359 5217 388
Size: 3971 858 1824
Fragments arranged by size:
3971 1824 858
EaeI y'GGCC_r
Cuts at: 1901 3525 4238 4412 4803 4830 6175 1901
Size: 1624 713 174 391 27 1345 2379
Fragments arranged by size:
2379 1624 1345 713 391 174 27
EagI C'GGCC_G
Cuts at: 4238 4238
Size: 6653
EarI CTCTTCn'nnn_
Cuts at: 175 1544 2230 4676 4886 175
Size: 1369 686 2446 210 1942
Fragments arranged by size:
2446 1942 1369 686 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 514 1917 4861 5171 5317 6463 514
Size: 1403 2944 310 146 1146 704
Fragments arranged by size:
2944 1403 1146 704 310 146
EcoICRI GAG'CTC
Cuts at: 3665 3665
Size: 6653
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 851 940 1816 1952 2165 2374 2591 2876 3443
Size: 89 876 136 213 209 217 285 567
Cuts at: 3443 3611 4478 4910 5657 5925 851
Size: 168 867 432 747 268 1579
Fragments arranged by size:
1579 876 867 747 567 432 285 268
217 213 209 168 136 89
EcoNI CCTnn'n_nnAGG
Cuts at: 407 733 407
Size: 326 6327
EcoO109I rG'GnC_Cy
Cuts at: 708 1184 1886 2517 2649 2859 3046 3792 708
Size: 476 702 631 132 210 187 746 3569
Fragments arranged by size:
3569 746 702 631 476 210 187 132
EcoRI G'AATT_C
Cuts at: 3636 3657 3636
Size: 21 6632
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 156 223 577 4672 156
Size: 67 354 4095 2137
Fragments arranged by size:
4095 2137 354 67
FspI TGC'GCA
Cuts at: 4434 4434
Size: 6653
HaeII r_GCGC'y
Cuts at: 141 1937 2412 4087 4335 5357 6336 141
Size: 1796 475 1675 248 1022 979 458
Fragments arranged by size:
1796 1675 1022 979 475 458 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1909 1941 2919 2951 4495 4525 4527 4557 1909
Size: 32 978 32 1544 30 2 30 4005
Fragments arranged by size:
4005 1544 978 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1235 1839 3504 3627 3692 1235
Size: 604 1665 123 65 4196
Fragments arranged by size:
4196 1665 604 123 65
HindIII A'AGCT_T
Cuts at: 336 336
Size: 6653
HpaI GTT'AAC
Cuts at: 1235 1235
Size: 6653
KasI G'GCGC_C
Cuts at: 2408 4083 4331 2408
Size: 1675 248 4730
Fragments arranged by size:
4730 1675 248
KpnI G_GTAC'C
Cuts at: 3673 3673
Size: 6653
MfeI C'AATT_G
Cuts at: 617 617
Size: 6653
MluI A'CGCG_T
Cuts at: 6086 6086
Size: 6653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 268 1987 2962 5324 5508 268
Size: 1719 975 2362 184 1413
Fragments arranged by size:
2362 1719 1413 975 184
MscI TGG'CCA
Cuts at: 1903 3527 4414 1903
Size: 1624 887 4142
Fragments arranged by size:
4142 1624 887
MslI CAynn'nnrTG
Cuts at: 543 921 1568 1897 2305 2984 4642 4769 543
Size: 378 647 329 408 679 1658 127 2427
Fragments arranged by size:
2427 1658 679 647 408 378 329 127
MspA1I CmG'CkG
Cuts at: 1883 2043 2175 2305 2551 3764 4078 4438 5451
Size: 160 132 130 246 1213 314 360 1013
Cuts at: 5451 5696 1883
Size: 245 2840
Fragments arranged by size:
2840 1213 1013 360 314 246 245 160
132 130
NaeI GCC'GGC
Cuts at: 3214 3587 4834 3214
Size: 373 1247 5033
Fragments arranged by size:
5033 1247 373
NarI GG'CG_CC
Cuts at: 2409 4084 4332 2409
Size: 1675 248 4730
Fragments arranged by size:
4730 1675 248
NgoMIV G'CCGG_C
Cuts at: 3212 3585 4832 3212
Size: 373 1247 5033
Fragments arranged by size:
5033 1247 373
NsiI A_TGCA'T
Cuts at: 1086 1086
Size: 6653
NspI r_CATG'y
Cuts at: 2793 3706 4206 5113 2793
Size: 913 500 907 4333
Fragments arranged by size:
4333 913 907 500
PciI A'CATG_T
Cuts at: 5109 5109
Size: 6653
PflMI CCAn_nnn'nTGG
Cuts at: 1511 1511
Size: 6653
PfoI T'CCnGG_A
Cuts at: 343 343
Size: 6653
PmeI GTTT'AAAC
Cuts at: 3652 3652
Size: 6653
PmlI CAC'GTG
Cuts at: 1895 1895
Size: 6653
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5792 5824 5792
Size: 32 6621
PpuMI rG'GwC_Cy
Cuts at: 708 1184 1886 2649 708
Size: 476 702 763 4712
Fragments arranged by size:
4712 763 702 476
PsiI TTA'TAA
Cuts at: 1004 1004
Size: 6653
PspOMI G'GGCC_C
Cuts at: 2859 2859
Size: 6653
PstI C_TGCA'G
Cuts at: 504 610 1198 3700 4385 504
Size: 106 588 2502 685 2772
Fragments arranged by size:
2772 2502 685 588 106
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6653
PvuII CAG'CTG
Cuts at: 2043 2175 2305 2551 4078 4438 2043
Size: 132 130 246 1527 360 4258
Fragments arranged by size:
4258 1527 360 246 132 130
RsrII CG'GwC_CG
Cuts at: 4848 4848
Size: 6653
SacI G_AGCT'C
Cuts at: 3667 3667
Size: 6653
SalI G'TCGA_C
Cuts at: 3690 3690
Size: 6653
SanDI GG'GwC_CC
Cuts at: 2649 2649
Size: 6653
SapI GCTCTTCn'nnn_
Cuts at: 1544 2230 4676 4886 1544
Size: 686 2446 210 3311
Fragments arranged by size:
3311 2446 686 210
SbfI CC_TGCA'GG
Cuts at: 3700 3700
Size: 6653
ScaI AGT'ACT
Cuts at: 599 6170 599
Size: 5571 1082
SexAI A'CCwGG_T
Cuts at: 1442 1442
Size: 6653
SfcI C'TryA_G
Cuts at: 33 500 606 1194 2552 3696 4381 5374 5565
Size: 467 106 588 1358 1144 685 993 191
Cuts at: 5565 33
Size: 1121
Fragments arranged by size:
1358 1144 1121 993 685 588 467 191
106
SfoI GGC'GCC
Cuts at: 2410 4085 4333 2410
Size: 1675 248 4730
Fragments arranged by size:
4730 1675 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6653
SmaI CCC'GGG
Cuts at: 3675 3675
Size: 6653
SmlI C'TyrA_G
Cuts at: 692 3808 4723 5215 5477 692
Size: 3116 915 492 262 1868
Fragments arranged by size:
3116 1868 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6653
SphI G_CATG'C
Cuts at: 3706 4206 3706
Size: 500 6153
SspI AAT'ATT
Cuts at: 297 641 297
Size: 344 6309
StuI AGG'CCT
Cuts at: 1461 2387 1461
Size: 926 5727
StyI C'CwwG_G
Cuts at: 2769 3787 4141 2769
Size: 1018 354 5281
Fragments arranged by size:
5281 1018 354
SwaI ATTT'AAAT
Cuts at: 3104 3104
Size: 6653
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 268 4236 4369 4828 5037 268
Size: 3968 133 459 209 1884
Fragments arranged by size:
3968 1884 459 209 133
TatI w'GTAC_w
Cuts at: 475 597 1606 2393 3002 6168 475
Size: 122 1009 787 609 3166 960
Fragments arranged by size:
3166 1009 960 787 609 122
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1400 1432 1759 1791 2592 2624 2816 2848 6170
Size: 32 327 32 801 32 192 32 3322
Cuts at: 6170 6202 1400
Size: 32 1851
Fragments arranged by size:
3322 1851 801 327 192 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1921 2878 4450 1921
Size: 957 1572 4124
Fragments arranged by size:
4124 1572 957
XbaI T'CTAG_A
Cuts at: 3684 3684
Size: 6653
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 341 956 1508 1911 4148 341
Size: 615 552 403 2237 2846
Fragments arranged by size:
2846 2237 615 552 403
XmaI C'CCGG_G
Cuts at: 3673 3673
Size: 6653
XmnI GAAnn'nnTTC
Cuts at: 2365 3456 2365
Size: 1091 5562
ZraI GAC'GTC
Cuts at: 2740 2740
Size: 6653
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgZI BtsI ClaI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PsiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AhdI AlfI AloI AscI AvrII BaeI BbvCI
BglI BmgBI BmtI BsiWI BssHII BstZ17I Bsu36I BtgI
CspCI EcoRV FseI FspAI NcoI NdeI NheI NotI
NruI PacI PasI PshAI PspXI PsrI SacII SfiI
SgrAI SnaBI SrfI XhoI