Restriction map for
pF1KA0350
(Circular) (Six-base) MAPSORT of: pF1KA0350.seq Check: 8497 from: 1 to: 6272
pF1KA0350 6272 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 896 962 1525 2373 2666 896
Size: 66 563 848 293 4502
Fragments arranged by size:
4502 848 563 293 66
AccI GT'mk_AC
Cuts at: 3310 3310
Size: 6272
Acc65I G'GTAC_C
Cuts at: 3288 3288
Size: 6272
AclI AA'CG_TT
Cuts at: 1292 6112 1292
Size: 4820 1452
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1130 1834 2633 4097 4529 5276 5544 1130
Size: 704 799 1464 432 747 268 1858
Fragments arranged by size:
1858 1464 799 747 704 432 268
AfeI AGC'GCT
Cuts at: 2716 2716
Size: 6272
AflIII A'CryG_T
Cuts at: 383 983 1943 3624 4728 5705 383
Size: 600 960 1681 1104 977 950
Fragments arranged by size:
1681 1104 977 960 950 600
AgeI A'CCGG_T
Cuts at: 4660 4660
Size: 6272
AleI CACnn'nnGTG
Cuts at: 1468 1468
Size: 6272
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1486 2581 1486
Size: 1095 5177
AlwNI CAG_nnn'CTG
Cuts at: 399 2529 2658 2855 3577 5144 399
Size: 2130 129 197 722 1567 1527
Fragments arranged by size:
2130 1567 1527 722 197 129
ApaI G_GGCC'C
Cuts at: 1325 1325
Size: 6272
ApaLI G'TGCA_C
Cuts at: 1858 5042 1858
Size: 3184 3088
ApoI r'AATT_y
Cuts at: 477 496 3276 5625 5881 477
Size: 19 2780 2349 256 868
Fragments arranged by size:
2780 2349 868 256 19
AseI AT'TA_AT
Cuts at: 20 20
Size: 6272
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6272
AvaI C'yCGr_G
Cuts at: 1222 2180 3292 1222
Size: 958 1112 4202
Fragments arranged by size:
4202 1112 958
BamHI G'GATC_C
Cuts at: 1650 3297 1650
Size: 1647 4625
BanI G'GyrC_C
Cuts at: 2684 2742 2869 3253 3288 3702 3950 3985 2684
Size: 58 127 384 35 414 248 35 4971
Fragments arranged by size:
4971 414 384 248 127 58 35 35
BanII G_rGCy'C
Cuts at: 1325 2830 3218 3286 4316 1325
Size: 1505 388 68 1030 3281
Fragments arranged by size:
3281 1505 1030 388 68
BbeI G_GCGC'C
Cuts at: 2688 3706 3954 2688
Size: 1018 248 5006
Fragments arranged by size:
5006 1018 248
BbsI GAAGACnn'nnnn_
Cuts at: 1059 1349 1708 1934 2098 1059
Size: 290 359 226 164 5233
Fragments arranged by size:
5233 359 290 226 164
BbvCI CC'TCA_GC
Cuts at: 3260 3260
Size: 6272
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 826 860 2040 2074 2221 2255 2392 2426 3214
Size: 34 1180 34 147 34 137 34 788
Cuts at: 3214 3248 826
Size: 34 3850
Fragments arranged by size:
3850 1180 788 147 137 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4170 4931 6272 4170
Size: 761 1341 4170
Fragments arranged by size:
4170 1341 761
BclI T'GATC_A
Cuts at: 911 1424 2195 2252 3792 911
Size: 513 771 57 1540 3391
Fragments arranged by size:
3391 1540 771 513 57
BglI GCCn_nnn'nGGC
Cuts at: 1354 2834 3173 1354
Size: 1480 339 4453
Fragments arranged by size:
4453 1480 339
BglII A'GATC_T
Cuts at: 3787 5824 3787
Size: 2037 4235
BlpI GC'TnA_GC
Cuts at: 1798 3112 3242 3384 1798
Size: 1314 130 142 4686
Fragments arranged by size:
4686 1314 142 130
Bme1580I G_kGCm'C
Cuts at: 1325 1862 2415 3256 3897 3990 5046 1325
Size: 537 553 841 641 93 1056 2551
Fragments arranged by size:
2551 1056 841 641 553 537 93
BmgBI CAC'GTC
Cuts at: 1857 1857
Size: 6272
BmrI ACTGGGnnnn_n'
Cuts at: 2757 3136 3484 3565 3900 6044 2757
Size: 379 348 81 335 2144 2985
Fragments arranged by size:
2985 2144 379 348 335 81
BmtI G_CTAG'C
Cuts at: 1726 1726
Size: 6272
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4639 4639
Size: 6272
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 1015 1813 1894 2458 2885 346
Size: 669 798 81 564 427 3733
Fragments arranged by size:
3733 798 669 564 427 81
Bpu10I CC'TnA_GC
Cuts at: 2939 3260 2939
Size: 321 5951
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 1255 3448 4327 4819 5117 169
Size: 1086 2193 879 492 298 1324
Fragments arranged by size:
2193 1324 1086 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 247 433 1269 5976 247
Size: 186 836 4707 543
Fragments arranged by size:
4707 836 543 186
BsaAI yAC'GTr
Cuts at: 384 474 984 3625 384
Size: 90 510 2641 3031
Fragments arranged by size:
3031 2641 510 90
BsaBI GATnn'nnATC
Cuts at: 6 280 1423 3786 5823 6
Size: 274 1143 2363 2037 455
Fragments arranged by size:
2363 2037 1143 455 274
BsaHI Gr'CG_yC
Cuts at: 1346 2009 2685 3703 3951 4653 6149 1346
Size: 663 676 1018 248 702 1496 1469
Fragments arranged by size:
1496 1469 1018 702 676 663 248
BsaWI w'CCGG_w
Cuts at: 236 3461 3982 4660 4934 5081 5492 236
Size: 3225 521 678 274 147 411 1016
Fragments arranged by size:
3225 1016 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 154 184 154
Size: 30 6242
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 529 847 1028 1327 1510 1609 2200 2628 529
Size: 318 181 299 183 99 591 428 4173
Fragments arranged by size:
4173 591 428 318 299 183 181 99
BseYI C'CCAG_C
Cuts at: 128 1186 1760 2342 2830 2998 3049 3697 4455
Size: 1058 574 582 488 168 51 648 758
Cuts at: 4455 5032 5528 128
Size: 577 496 872
Fragments arranged by size:
1058 872 758 648 582 577 574 496
488 168 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 715 841 874 1492 2731 3192 715
Size: 126 33 618 1239 461 3795
Fragments arranged by size:
3795 1239 618 461 126 33
BsiEI CG_ry'CG
Cuts at: 104 1024 1697 3814 3860 5068 104
Size: 920 673 2117 46 1208 1308
Fragments arranged by size:
2117 1308 1208 920 673 46
BsiHKAI G_wGCw'C
Cuts at: 602 878 944 1531 1613 1862 2848 3286 4064
Size: 276 66 587 82 249 986 438 778
Cuts at: 4064 4254 5046 602
Size: 190 792 1828
Fragments arranged by size:
1828 986 792 778 587 438 276 249
190 82 66
BsmI GAATG_Cn'
Cuts at: 1190 1190
Size: 6272
Bsp1286I G_dGCh'C
Cuts at: 602 878 944 1325 1531 1613 1862 2415 2830
Size: 276 66 381 206 82 249 553 415
Cuts at: 2830 2848 3218 3256 3286 3897 3990 4064 4254
Size: 18 370 38 30 611 93 74 190
Cuts at: 4254 4316 5046 602
Size: 62 730 1828
Fragments arranged by size:
1828 730 611 553 415 381 370 276
249 206 190 93 82 74 66 62
38 30 18
BspEI T'CCGG_A
Cuts at: 5492 5492
Size: 6272
BspHI T'CATG_A
Cuts at: 1745 1847 2000 5448 1745
Size: 102 153 3448 2569
Fragments arranged by size:
3448 2569 153 102
BspMI ACCTGCnnnn'nnnn_
Cuts at: 251 896 962 1525 2373 2666 3322 3536 3838
Size: 645 66 563 848 293 656 214 302
Cuts at: 3838 4219 251
Size: 381 2304
Fragments arranged by size:
2304 848 656 645 563 381 302 293
214 66
BsrBI CCG'CTC
Cuts at: 50 119 1227 4564 4618 6102 6267 50
Size: 69 1108 3337 54 1484 165 55
Fragments arranged by size:
3337 1484 1108 165 69 55 54
BsrDI GCAATG_nn'
Cuts at: 1540 4184 1540
Size: 2644 3628
BsrFI r'CCGG_y
Cuts at: 1038 3172 3195 4270 4451 4660 1038
Size: 2134 23 1075 181 209 2650
Fragments arranged by size:
2650 2134 1075 209 181 23
BsrGI T'GTAC_A
Cuts at: 620 2245 620
Size: 1625 4647
BssSI C'ACGA_G
Cuts at: 436 4543 4901 436
Size: 4107 358 1807
Fragments arranged by size:
4107 1807 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1196 2379 3649 1196
Size: 1183 1270 3819
Fragments arranged by size:
3819 1270 1183
BstBI TT'CG_AA
Cuts at: 4633 4633
Size: 6272
BstXI CCAn_nnnn'nTGG
Cuts at: 1768 1989 1768
Size: 221 6051
BstYI r'GATC_y
Cuts at: 1 275 1650 1775 2217 3297 3764 3787 4122
Size: 274 1375 125 442 1080 467 23 335
Cuts at: 4122 4368 5380 5466 5478 5824 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1375 1080 1012 467 449 442 346 335
274 246 125 86 23 12
BtgI C'CryG_G
Cuts at: 144 1487 2756 3026 3218 144
Size: 1343 1269 270 192 3198
Fragments arranged by size:
3198 1343 1269 270 192
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2096 2499 4222 4403 4566 2096
Size: 403 1723 181 163 3802
Fragments arranged by size:
3802 1723 403 181 163
BtsI GCAGTG_nn'
Cuts at: 2137 2853 3515 2137
Size: 716 662 4894
Fragments arranged by size:
4894 716 662
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1377 1412 1377
Size: 35 6237
DraI TTT'AAA
Cuts at: 3271 3271
Size: 6272
DrdI GACnn_nn'nnGTC
Cuts at: 3978 4836 3978
Size: 858 5414
EaeI y'GGCC_r
Cuts at: 114 1021 1454 1694 2531 3011 3857 4031 4422
Size: 907 433 240 837 480 846 174 391
Cuts at: 4422 4449 5794 114
Size: 27 1345 592
Fragments arranged by size:
1345 907 846 837 592 480 433 391
240 174 27
EagI C'GGCC_G
Cuts at: 1021 1694 3857 1021
Size: 673 2163 3436
Fragments arranged by size:
3436 2163 673
EarI CTCTTCn'nnn_
Cuts at: 973 1403 3221 4295 4505 973
Size: 430 1818 1074 210 2740
Fragments arranged by size:
2740 1818 1074 430 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2805 4480 4790 4936 6082 2805
Size: 1675 310 146 1146 2995
Fragments arranged by size:
2995 1675 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3284 3284
Size: 6272
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 1015 1130 1813 1834 1894 2458 2633 2885
Size: 669 115 683 21 60 564 175 252
Cuts at: 2885 4097 4529 5276 5544 346
Size: 1212 432 747 268 1074
Fragments arranged by size:
1212 1074 747 683 669 564 432 268
252 175 115 60 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2371 2371
Size: 6272
EcoO109I rG'GnC_Cy
Cuts at: 1321 2190 3411 1321
Size: 869 1221 4182
Fragments arranged by size:
4182 1221 869
EcoRI G'AATT_C
Cuts at: 3276 3276
Size: 6272
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4291 4291
Size: 6272
FspI TGC'GCA
Cuts at: 4053 4053
Size: 6272
HaeII r_GCGC'y
Cuts at: 1508 2529 2688 2718 3706 3954 4976 5955 1508
Size: 1021 159 30 988 248 1022 979 1825
Fragments arranged by size:
1825 1022 1021 988 979 248 159 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 4114 4144 4146 4176 4114
Size: 30 2 30 6210
Fragments arranged by size:
6210 30 30 2
HincII GTy'rAC
Cuts at: 2973 3147 3311 2973
Size: 174 164 5934
Fragments arranged by size:
5934 174 164
KasI G'GCGC_C
Cuts at: 2684 3702 3950 2684
Size: 1018 248 5006
Fragments arranged by size:
5006 1018 248
KpnI G_GTAC'C
Cuts at: 3292 3292
Size: 6272
MfeI C'AATT_G
Cuts at: 2143 2143
Size: 6272
MluI A'CGCG_T
Cuts at: 5705 5705
Size: 6272
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4943 5127 4943
Size: 184 6088
MscI TGG'CCA
Cuts at: 2533 3013 4033 2533
Size: 480 1020 4772
Fragments arranged by size:
4772 1020 480
MslI CAynn'nnrTG
Cuts at: 605 1468 1972 1987 2110 2383 2563 3175 4261
Size: 863 504 15 123 273 180 612 1086
Cuts at: 4261 4388 605
Size: 127 2489
Fragments arranged by size:
2489 1086 863 612 504 273 180 127
123 15
MspA1I CmG'CkG
Cuts at: 396 1092 2175 2507 2822 2834 2840 3186 3259
Size: 696 1083 332 315 12 6 346 73
Cuts at: 3259 3383 3697 4057 5070 5315 396
Size: 124 314 360 1013 245 1353
Fragments arranged by size:
1353 1083 1013 696 360 346 332 315
314 245 124 73 12 6
NaeI GCC'GGC
Cuts at: 3197 4453 3197
Size: 1256 5016
NarI GG'CG_CC
Cuts at: 2685 3703 3951 2685
Size: 1018 248 5006
Fragments arranged by size:
5006 1018 248
NcoI C'CATG_G
Cuts at: 144 144
Size: 6272
NgoMIV G'CCGG_C
Cuts at: 3195 4451 3195
Size: 1256 5016
NheI G'CTAG_C
Cuts at: 1722 1722
Size: 6272
NspI r_CATG'y
Cuts at: 1947 2246 2382 3180 3239 3325 3825 4732 1947
Size: 299 136 798 59 86 500 907 3487
Fragments arranged by size:
3487 907 798 500 299 136 86 59
PciI A'CATG_T
Cuts at: 1943 4728 1943
Size: 2785 3487
PflMI CCAn_nnn'nTGG
Cuts at: 174 1572 174
Size: 1398 4874
PfoI T'CCnGG_A
Cuts at: 323 1550 2213 2585 323
Size: 1227 663 372 4010
Fragments arranged by size:
4010 1227 663 372
PmeI GTTT'AAAC
Cuts at: 3271 3271
Size: 6272
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5411 5443 5411
Size: 32 6240
PpuMI rG'GwC_Cy
Cuts at: 2190 2190
Size: 6272
PshAI GACnn'nnGTC
Cuts at: 3133 3133
Size: 6272
PsiI TTA'TAA
Cuts at: 593 1919 593
Size: 1326 4946
PspOMI G'GGCC_C
Cuts at: 1321 1321
Size: 6272
PspXI vC'TCGA_Gb
Cuts at: 1222 1222
Size: 6272
PstI C_TGCA'G
Cuts at: 404 3319 4004 404
Size: 2915 685 2672
Fragments arranged by size:
2915 2672 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6272
PvuII CAG'CTG
Cuts at: 396 3697 4057 396
Size: 3301 360 2611
Fragments arranged by size:
3301 2611 360
RsrII CG'GwC_CG
Cuts at: 4467 4467
Size: 6272
SacI G_AGCT'C
Cuts at: 3286 3286
Size: 6272
SalI G'TCGA_C
Cuts at: 3309 3309
Size: 6272
SapI GCTCTTCn'nnn_
Cuts at: 973 4295 4505 973
Size: 3322 210 2740
Fragments arranged by size:
3322 2740 210
SbfI CC_TGCA'GG
Cuts at: 3319 3319
Size: 6272
ScaI AGT'ACT
Cuts at: 5789 5789
Size: 6272
SfcI C'TryA_G
Cuts at: 33 400 3315 4000 4993 5184 33
Size: 367 2915 685 993 191 1121
Fragments arranged by size:
2915 1121 993 685 367 191
SfoI GGC'GCC
Cuts at: 2686 3704 3952 2686
Size: 1018 248 5006
Fragments arranged by size:
5006 1018 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6272
SmaI CCC'GGG
Cuts at: 3294 3294
Size: 6272
SmlI C'TyrA_G
Cuts at: 184 1222 1234 3427 4342 4834 5096 184
Size: 1038 12 2193 915 492 262 1360
Fragments arranged by size:
2193 1360 1038 915 492 262 12
SnaBI TAC'GTA
Cuts at: 474 474
Size: 6272
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6272
SphI G_CATG'C
Cuts at: 3180 3325 3825 3180
Size: 145 500 5627
Fragments arranged by size:
5627 500 145
StuI AGG'CCT
Cuts at: 2366 2366
Size: 6272
StyI C'CwwG_G
Cuts at: 144 173 2258 3406 3760 144
Size: 29 2085 1148 354 2656
Fragments arranged by size:
2656 2085 1148 354 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 120 3855 3988 4447 4656 120
Size: 3735 133 459 209 1736
Fragments arranged by size:
3735 1736 459 209 133
TatI w'GTAC_w
Cuts at: 620 986 2245 5787 620
Size: 366 1259 3542 1105
Fragments arranged by size:
3542 1259 1105 366
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5789 5821 5789
Size: 32 6240
Tth111I GACn'n_nGTC
Cuts at: 4069 4069
Size: 6272
XbaI T'CTAG_A
Cuts at: 3303 3303
Size: 6272
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2526 2616 2860 3006 3767 2526
Size: 90 244 146 761 5031
Fragments arranged by size:
5031 761 244 146 90
XhoI C'TCGA_G
Cuts at: 1222 1222
Size: 6272
XmaI C'CCGG_G
Cuts at: 3292 3292
Size: 6272
XmnI GAAnn'nnTTC
Cuts at: 1115 1895 1115
Size: 780 5492
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI AvrII BaeI BfrBI
BsiWI BsmBI BssHII BstEII BstZ17I Bsu36I ClaI DraIII
EcoRV FseI FspAI HindIII HpaI NdeI NotI NruI
NsiI PacI PasI PmlI PsrI SacII SanDI SexAI
SfiI SgrAI SrfI SspI SwaI ZraI