Restriction map for
pF1KA0351
(Circular) (Six-base) MAPSORT of: pF1KA0351.seq Check: 6573 from: 1 to: 4784
pF1KA0351 4784 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 1822 1822
Size: 4784
Acc65I G'GTAC_C
Cuts at: 1752 1800 1752
Size: 48 4736
AclI AA'CG_TT
Cuts at: 377 4624 377
Size: 4247 537
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 552 709 913 1325 2609 3041 3788 4056 552
Size: 157 204 412 1284 432 747 268 1280
Fragments arranged by size:
1284 1280 747 432 412 268 204 157
AflIII A'CryG_T
Cuts at: 2136 3240 4217 2136
Size: 1104 977 2703
Fragments arranged by size:
2703 1104 977
AgeI A'CCGG_T
Cuts at: 3172 3172
Size: 4784
AlwNI CAG_nnn'CTG
Cuts at: 1481 2089 3656 1481
Size: 608 1567 2609
Fragments arranged by size:
2609 1567 608
ApaLI G'TGCA_C
Cuts at: 3554 3554
Size: 4784
ApoI r'AATT_y
Cuts at: 431 1788 4137 4393 431
Size: 1357 2349 256 822
Fragments arranged by size:
2349 1357 822 256
AseI AT'TA_AT
Cuts at: 20 286 20
Size: 266 4518
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4784
AvaI C'yCGr_G
Cuts at: 1618 1804 1618
Size: 186 4598
AvrII C'CTAG_G
Cuts at: 1134 1299 1134
Size: 165 4619
BamHI G'GATC_C
Cuts at: 1498 1809 1498
Size: 311 4473
BanI G'GyrC_C
Cuts at: 1208 1752 1800 2214 2462 2497 1208
Size: 544 48 414 248 35 3495
Fragments arranged by size:
3495 544 414 248 48 35
BanII G_rGCy'C
Cuts at: 591 920 1318 1798 2828 591
Size: 329 398 480 1030 2547
Fragments arranged by size:
2547 1030 480 398 329
BbeI G_GCGC'C
Cuts at: 2218 2466 2218
Size: 248 4536
BbsI GAAGACnn'nnnn_
Cuts at: 228 941 1331 228
Size: 713 390 3681
Fragments arranged by size:
3681 713 390
BbvCI CC'TCA_GC
Cuts at: 472 472
Size: 4784
BciVI GTATCCnnnnn_n'
Cuts at: 2682 3443 4784 2682
Size: 761 1341 2682
Fragments arranged by size:
2682 1341 761
BclI T'GATC_A
Cuts at: 855 2304 855
Size: 1449 3335
BfrBI ATG'CAT
Cuts at: 1729 1729
Size: 4784
BglI GCCn_nnn'nGGC
Cuts at: 1361 1361
Size: 4784
BglII A'GATC_T
Cuts at: 1008 2299 4336 1008
Size: 1291 2037 1456
Fragments arranged by size:
2037 1456 1291
BlpI GC'TnA_GC
Cuts at: 1896 1896
Size: 4784
Bme1580I G_kGCm'C
Cuts at: 1403 1540 2409 2502 3558 1403
Size: 137 869 93 1056 2629
Fragments arranged by size:
2629 1056 869 137 93
BmrI ACTGGGnnnn_n'
Cuts at: 1491 1996 2077 2412 4556 1491
Size: 505 81 335 2144 1719
Fragments arranged by size:
2144 1719 505 335 81
BmtI G_CTAG'C
Cuts at: 137 275 137
Size: 138 4646
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3151 3151
Size: 4784
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 904 1088 208
Size: 696 184 3904
Fragments arranged by size:
3904 696 184
Bpu10I CC'TnA_GC
Cuts at: 472 472
Size: 4784
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1423 1960 2839 3331 3629 1423
Size: 537 879 492 298 2578
Fragments arranged by size:
2578 879 537 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4488 4488
Size: 4784
BsaAI yAC'GTr
Cuts at: 2137 2137
Size: 4784
BsaBI GATnn'nnATC
Cuts at: 6 2298 4335 6
Size: 2292 2037 455
Fragments arranged by size:
2292 2037 455
BsaHI Gr'CG_yC
Cuts at: 2215 2463 3165 4661 2215
Size: 248 702 1496 2338
Fragments arranged by size:
2338 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1033 1973 2494 3172 3446 3593 4004 1033
Size: 940 521 678 274 147 411 1813
Fragments arranged by size:
1813 940 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 277 1260 1263 277
Size: 983 3 3798
Fragments arranged by size:
3798 983 3
BseYI C'CCAG_C
Cuts at: 2209 2967 3544 4040 2209
Size: 758 577 496 2953
Fragments arranged by size:
2953 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 901 1621 901
Size: 720 4064
BsiEI CG_ry'CG
Cuts at: 104 2326 2372 3580 104
Size: 2222 46 1208 1308
Fragments arranged by size:
2222 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 561 920 1318 1625 1798 2576 2766 3558 561
Size: 359 398 307 173 778 190 792 1787
Fragments arranged by size:
1787 792 778 398 359 307 190 173
BsiWI C'GTAC_G
Cuts at: 900 900
Size: 4784
Bsp1286I G_dGCh'C
Cuts at: 561 591 920 1318 1403 1540 1625 1798 2409
Size: 30 329 398 85 137 85 173 611
Cuts at: 2409 2502 2576 2766 2828 3558 561
Size: 93 74 190 62 730 1787
Fragments arranged by size:
1787 730 611 398 329 190 173 137
93 85 85 74 62 30
BspEI T'CCGG_A
Cuts at: 4004 4004
Size: 4784
BspHI T'CATG_A
Cuts at: 3960 3960
Size: 4784
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1006 1764 1834 2048 2350 2731 1006
Size: 758 70 214 302 381 3059
Fragments arranged by size:
3059 758 381 302 214 70
BsrBI CCG'CTC
Cuts at: 50 3076 3130 4614 4779 50
Size: 3026 54 1484 165 55
Fragments arranged by size:
3026 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1668 2696 1668
Size: 1028 3756
BsrFI r'CCGG_y
Cuts at: 1360 2782 2963 3172 1360
Size: 1422 181 209 2972
Fragments arranged by size:
2972 1422 209 181
BsrGI T'GTAC_A
Cuts at: 110 110
Size: 4784
BssSI C'ACGA_G
Cuts at: 1467 3055 3413 1467
Size: 1588 358 2838
Fragments arranged by size:
2838 1588 358
BstAPI GCAn_nnn'nTGC
Cuts at: 760 2161 760
Size: 1401 3383
BstBI TT'CG_AA
Cuts at: 819 3145 819
Size: 2326 2458
BstEII G'GTnAC_C
Cuts at: 144 144
Size: 4784
BstYI r'GATC_y
Cuts at: 1 793 1008 1498 1809 2276 2299 2634 2880
Size: 792 215 490 311 467 23 335 246
Cuts at: 2880 3892 3978 3990 4336 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 792 490 467 449 346 335 311
246 215 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 765 1748 765
Size: 983 3801
BtgI C'CryG_G
Cuts at: 1406 1406
Size: 4784
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2734 2915 3078 2734
Size: 181 163 4440
Fragments arranged by size:
4440 181 163
BtsI GCAGTG_nn'
Cuts at: 2027 2027
Size: 4784
DraI TTT'AAA
Cuts at: 306 452 596 1783 306
Size: 146 144 1187 3307
Fragments arranged by size:
3307 1187 146 144
DrdI GACnn_nn'nnGTC
Cuts at: 1225 2490 3348 1225
Size: 1265 858 2661
Fragments arranged by size:
2661 1265 858
EaeI y'GGCC_r
Cuts at: 2369 2543 2934 2961 4306 2369
Size: 174 391 27 1345 2847
Fragments arranged by size:
2847 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2369 2369
Size: 4784
EarI CTCTTCn'nnn_
Cuts at: 908 1002 1117 1325 2807 3017 908
Size: 94 115 208 1482 210 2675
Fragments arranged by size:
2675 1482 210 208 115 94
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1464 2992 3302 3448 4594 1464
Size: 1528 310 146 1146 1654
Fragments arranged by size:
1654 1528 1146 310 146
EcoICRI GAG'CTC
Cuts at: 918 1316 1796 918
Size: 398 480 3906
Fragments arranged by size:
3906 480 398
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 552 709 904 913 1088 1325 2609 3041
Size: 344 157 195 9 175 237 1284 432
Cuts at: 3041 3788 4056 208
Size: 747 268 936
Fragments arranged by size:
1284 936 747 432 344 268 237 195
175 157 9
EcoNI CCTnn'n_nnAGG
Cuts at: 764 1438 1655 764
Size: 674 217 3893
Fragments arranged by size:
3893 674 217
EcoO109I rG'GnC_Cy
Cuts at: 1018 1254 1414 1923 1018
Size: 236 160 509 3879
Fragments arranged by size:
3879 509 236 160
EcoRI G'AATT_C
Cuts at: 1788 1788
Size: 4784
EcoRV GAT'ATC
Cuts at: 1632 1632
Size: 4784
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 633 2803 633
Size: 2170 2614
FspI TGC'GCA
Cuts at: 2565 2565
Size: 4784
HaeII r_GCGC'y
Cuts at: 2218 2466 3488 4467 2218
Size: 248 1022 979 2535
Fragments arranged by size:
2535 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 108 140 1490 1522 2626 2656 2658 2688 108
Size: 32 1350 32 1104 30 2 30 2204
Fragments arranged by size:
2204 1350 1104 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1823 1823
Size: 4784
KasI G'GCGC_C
Cuts at: 2214 2462 2214
Size: 248 4536
KpnI G_GTAC'C
Cuts at: 1756 1804 1756
Size: 48 4736
MluI A'CGCG_T
Cuts at: 4217 4217
Size: 4784
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3455 3639 3455
Size: 184 4600
MscI TGG'CCA
Cuts at: 2545 2545
Size: 4784
MslI CAynn'nnrTG
Cuts at: 535 1721 2773 2900 535
Size: 1186 1052 127 2419
Fragments arranged by size:
2419 1186 1052 127
MspA1I CmG'CkG
Cuts at: 335 1396 1605 1641 1895 2209 2569 3582 3827
Size: 1061 209 36 254 314 360 1013 245
Cuts at: 3827 335
Size: 1292
Fragments arranged by size:
1292 1061 1013 360 314 254 245 209
36
NaeI GCC'GGC
Cuts at: 2965 2965
Size: 4784
NarI GG'CG_CC
Cuts at: 2215 2463 2215
Size: 248 4536
NcoI C'CATG_G
Cuts at: 1406 1406
Size: 4784
NgoMIV G'CCGG_C
Cuts at: 2963 2963
Size: 4784
NheI G'CTAG_C
Cuts at: 133 271 133
Size: 138 4646
NsiI A_TGCA'T
Cuts at: 1731 1731
Size: 4784
NspI r_CATG'y
Cuts at: 1733 1837 2337 3244 1733
Size: 104 500 907 3273
Fragments arranged by size:
3273 907 500 104
PciI A'CATG_T
Cuts at: 3240 3240
Size: 4784
PflMI CCAn_nnn'nTGG
Cuts at: 1481 1481
Size: 4784
PmeI GTTT'AAAC
Cuts at: 1783 1783
Size: 4784
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3923 3955 3923
Size: 32 4752
PpuMI rG'GwC_Cy
Cuts at: 1018 1018
Size: 4784
PstI C_TGCA'G
Cuts at: 850 1831 2516 850
Size: 981 685 3118
Fragments arranged by size:
3118 981 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4784
PvuII CAG'CTG
Cuts at: 335 1396 1605 1641 2209 2569 335
Size: 1061 209 36 568 360 2550
Fragments arranged by size:
2550 1061 568 360 209 36
RsrII CG'GwC_CG
Cuts at: 2979 2979
Size: 4784
SacI G_AGCT'C
Cuts at: 920 1318 1798 920
Size: 398 480 3906
Fragments arranged by size:
3906 480 398
SalI G'TCGA_C
Cuts at: 1821 1821
Size: 4784
SapI GCTCTTCn'nnn_
Cuts at: 908 1117 2807 3017 908
Size: 209 1690 210 2675
Fragments arranged by size:
2675 1690 210 209
SbfI CC_TGCA'GG
Cuts at: 1831 1831
Size: 4784
ScaI AGT'ACT
Cuts at: 4301 4301
Size: 4784
SexAI A'CCwGG_T
Cuts at: 404 1474 404
Size: 1070 3714
SfcI C'TryA_G
Cuts at: 33 846 1827 2512 3505 3696 33
Size: 813 981 685 993 191 1121
Fragments arranged by size:
1121 993 981 813 685 191
SfoI GGC'GCC
Cuts at: 2216 2464 2216
Size: 248 4536
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4784
SmaI CCC'GGG
Cuts at: 1806 1806
Size: 4784
SmlI C'TyrA_G
Cuts at: 1438 1939 2854 3346 3608 1438
Size: 501 915 492 262 2614
Fragments arranged by size:
2614 915 501 492 262
SpeI A'CTAG_T
Cuts at: 67 990 67
Size: 923 3861
SphI G_CATG'C
Cuts at: 1837 2337 1837
Size: 500 4284
SspI AAT'ATT
Cuts at: 813 813
Size: 4784
StuI AGG'CCT
Cuts at: 1746 1746
Size: 4784
StyI C'CwwG_G
Cuts at: 1001 1134 1299 1406 1918 2272 1001
Size: 133 165 107 512 354 3513
Fragments arranged by size:
3513 512 354 165 133 107
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1640 2367 2500 2959 3168 1640
Size: 727 133 459 209 3256
Fragments arranged by size:
3256 727 459 209 133
TatI w'GTAC_w
Cuts at: 110 1511 4299 110
Size: 1401 2788 595
Fragments arranged by size:
2788 1401 595
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1091 1123 1543 1575 4301 4333 1091
Size: 32 420 32 2726 32 1542
Fragments arranged by size:
2726 1542 420 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1225 2581 1225
Size: 1356 3428
XbaI T'CTAG_A
Cuts at: 504 1815 504
Size: 1311 3473
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2279 2279
Size: 4784
XmaI C'CCGG_G
Cuts at: 1804 1804
Size: 4784
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NsiI NspI PciI PflMI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI BaeI BcgI BmgBI BsaXI BsmI BsmBI
BssHII BstXI BstZ17I ClaI CspCI DraIII FseI FspAI
HindIII HpaI MfeI NdeI NotI NruI PacI PasI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SwaI XhoI XmnI
ZraI