Restriction map for
pF1KA0354
(Circular) (Six-base) MAPSORT of: pF1KA0354.seq Check: 7608 from: 1 to: 5144
pF1KA0354 5144 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 431 1462 431
Size: 1031 4113
AccI GT'mk_AC
Cuts at: 361 2182 361
Size: 1821 3323
Acc65I G'GTAC_C
Cuts at: 2160 2160
Size: 5144
AclI AA'CG_TT
Cuts at: 4984 4984
Size: 5144
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 761 1571 2059 2969 3401 4148 4416 761
Size: 810 488 910 432 747 268 1489
Fragments arranged by size:
1489 910 810 747 488 432 268
AfeI AGC'GCT
Cuts at: 532 532
Size: 5144
AflIII A'CryG_T
Cuts at: 2496 3600 4577 2496
Size: 1104 977 3063
Fragments arranged by size:
3063 1104 977
AgeI A'CCGG_T
Cuts at: 3532 3532
Size: 5144
AleI CACnn'nnGTG
Cuts at: 2003 2015 2003
Size: 12 5132
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 122 154 122
Size: 32 5112
AlwNI CAG_nnn'CTG
Cuts at: 147 552 1462 2449 4016 147
Size: 405 910 987 1567 1275
Fragments arranged by size:
1567 1275 987 910 405
ApaI G_GGCC'C
Cuts at: 1481 1481
Size: 5144
ApaLI G'TGCA_C
Cuts at: 3914 3914
Size: 5144
ApoI r'AATT_y
Cuts at: 1338 2148 4497 4753 1338
Size: 810 2349 256 1729
Fragments arranged by size:
2349 1729 810 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5144
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5144
AvaI C'yCGr_G
Cuts at: 791 2164 791
Size: 1373 3771
BamHI G'GATC_C
Cuts at: 2169 2169
Size: 5144
BanI G'GyrC_C
Cuts at: 921 2160 2574 2822 2857 921
Size: 1239 414 248 35 3208
Fragments arranged by size:
3208 1239 414 248 35
BanII G_rGCy'C
Cuts at: 261 574 625 1114 1481 1759 1938 2158 3188
Size: 313 51 489 367 278 179 220 1030
Cuts at: 3188 261
Size: 2217
Fragments arranged by size:
2217 1030 489 367 313 278 220 179
51
BbeI G_GCGC'C
Cuts at: 2578 2826 2578
Size: 248 4896
BbvCI CC'TCA_GC
Cuts at: 1216 1216
Size: 5144
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2118 2152 2118
Size: 34 5110
BciVI GTATCCnnnnn_n'
Cuts at: 3042 3803 5144 3042
Size: 761 1341 3042
Fragments arranged by size:
3042 1341 761
BclI T'GATC_A
Cuts at: 888 1005 2664 888
Size: 117 1659 3368
Fragments arranged by size:
3368 1659 117
BglI GCCn_nnn'nGGC
Cuts at: 596 596
Size: 5144
BglII A'GATC_T
Cuts at: 2659 4696 2659
Size: 2037 3107
BlpI GC'TnA_GC
Cuts at: 573 2256 573
Size: 1683 3461
Bme1580I G_kGCm'C
Cuts at: 926 1481 2769 2862 3918 926
Size: 555 1288 93 1056 2152
Fragments arranged by size:
2152 1288 1056 555 93
BmrI ACTGGGnnnn_n'
Cuts at: 488 1248 2356 2437 2772 4916 488
Size: 760 1108 81 335 2144 716
Fragments arranged by size:
2144 1108 760 716 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3511 3511
Size: 5144
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1447 1644 1447
Size: 197 4947
Bpu10I CC'TnA_GC
Cuts at: 1118 1216 1118
Size: 98 5046
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 890 2320 3199 3691 3989 890
Size: 1430 879 492 298 2045
Fragments arranged by size:
2045 1430 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4848 4848
Size: 5144
BsaAI yAC'GTr
Cuts at: 2497 2497
Size: 5144
BsaBI GATnn'nnATC
Cuts at: 6 2658 4695 6
Size: 2652 2037 455
Fragments arranged by size:
2652 2037 455
BsaHI Gr'CG_yC
Cuts at: 672 2575 2823 3525 5021 672
Size: 1903 248 702 1496 795
Fragments arranged by size:
1903 1496 795 702 248
BsaWI w'CCGG_w
Cuts at: 762 2333 2854 3532 3806 3953 4364 762
Size: 1571 521 678 274 147 411 1542
Fragments arranged by size:
1571 1542 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1178
Size: 30 5114
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 616 715 811 1473 1616 1641 1703 616
Size: 99 96 662 143 25 62 4057
Fragments arranged by size:
4057 662 143 99 96 62 25
BseYI C'CCAG_C
Cuts at: 378 552 925 1462 1838 2125 2569 3327 3904
Size: 174 373 537 376 287 444 758 577
Cuts at: 3904 4400 378
Size: 496 1122
Fragments arranged by size:
1122 758 577 537 496 444 376 373
287 174
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 326 1001 1600 326
Size: 675 599 3870
Fragments arranged by size:
3870 675 599
BsiEI CG_ry'CG
Cuts at: 104 2686 2732 3940 104
Size: 2582 46 1208 1308
Fragments arranged by size:
2582 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 574 625 1079 1114 1759 1913 1938 2158 2936
Size: 51 454 35 645 154 25 220 778
Cuts at: 2936 3126 3918 574
Size: 190 792 1800
Fragments arranged by size:
1800 792 778 645 454 220 190 154
51 35 25
BsmBI CGTCTCn'nnnn_
Cuts at: 663 663
Size: 5144
Bsp1286I G_dGCh'C
Cuts at: 261 574 625 926 1079 1114 1481 1759 1913
Size: 313 51 301 153 35 367 278 154
Cuts at: 1913 1938 2158 2769 2862 2936 3126 3188 3918
Size: 25 220 611 93 74 190 62 730
Cuts at: 3918 261
Size: 1487
Fragments arranged by size:
1487 730 611 367 313 301 278 220
190 154 153 93 74 62 51 35
25
BspEI T'CCGG_A
Cuts at: 4364 4364
Size: 5144
BspHI T'CATG_A
Cuts at: 4320 4320
Size: 5144
BspMI ACCTGCnnnn'nnnn_
Cuts at: 431 1462 1533 2104 2194 2408 2710 3091 431
Size: 1031 71 571 90 214 302 381 2484
Fragments arranged by size:
2484 1031 571 381 302 214 90 71
BsrBI CCG'CTC
Cuts at: 50 224 705 3436 3490 4974 5139 50
Size: 174 481 2731 54 1484 165 55
Fragments arranged by size:
2731 1484 481 174 165 55 54
BsrDI GCAATG_nn'
Cuts at: 396 3056 396
Size: 2660 2484
BsrFI r'CCGG_y
Cuts at: 3142 3323 3532 3142
Size: 181 209 4754
Fragments arranged by size:
4754 209 181
BssSI C'ACGA_G
Cuts at: 977 3415 3773 977
Size: 2438 358 2348
Fragments arranged by size:
2438 2348 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1958 2521 1958
Size: 563 4581
BstBI TT'CG_AA
Cuts at: 1336 3505 1336
Size: 2169 2975
BstYI r'GATC_y
Cuts at: 1 1248 1763 2169 2636 2659 2994 3240 4252
Size: 1247 515 406 467 23 335 246 1012
Cuts at: 4252 4338 4350 4696 1
Size: 86 12 346 449
Fragments arranged by size:
1247 1012 515 467 449 406 346 335
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 362 362
Size: 5144
Bsu36I CC'TnA_GG
Cuts at: 1052 1124 1052
Size: 72 5072
BtgI C'CryG_G
Cuts at: 107 968 107
Size: 861 4283
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1154 3094 3275 3438 1154
Size: 1940 181 163 2860
Fragments arranged by size:
2860 1940 181 163
BtsI GCAGTG_nn'
Cuts at: 1236 2387 1236
Size: 1151 3993
DraI TTT'AAA
Cuts at: 213 2143 213
Size: 1930 3214
DrdI GACnn_nn'nnGTC
Cuts at: 2850 3708 2850
Size: 858 4286
EaeI y'GGCC_r
Cuts at: 168 1827 2729 2903 3294 3321 4666 168
Size: 1659 902 174 391 27 1345 646
Fragments arranged by size:
1659 1345 902 646 391 174 27
EagI C'GGCC_G
Cuts at: 2729 2729
Size: 5144
EarI CTCTTCn'nnn_
Cuts at: 630 3167 3377 630
Size: 2537 210 2397
Fragments arranged by size:
2537 2397 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3352 3662 3808 4954 3352
Size: 310 146 1146 3542
Fragments arranged by size:
3542 1146 310 146
EcoICRI GAG'CTC
Cuts at: 572 623 1112 1757 1936 2156 572
Size: 51 489 645 179 220 3560
Fragments arranged by size:
3560 645 489 220 179 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 761 1447 1571 1644 2059 2969 3401 4148 4416
Size: 686 124 73 415 910 432 747 268
Cuts at: 4416 761
Size: 1489
Fragments arranged by size:
1489 910 747 686 432 415 268 124
73
EcoNI CCTnn'n_nnAGG
Cuts at: 1659 1659
Size: 5144
EcoO109I rG'GnC_Cy
Cuts at: 1437 1478 2283 1437
Size: 41 805 4298
Fragments arranged by size:
4298 805 41
EcoRI G'AATT_C
Cuts at: 2148 2148
Size: 5144
EcoRV GAT'ATC
Cuts at: 1281 1281
Size: 5144
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1917 3163 1917
Size: 1246 3898
FspI TGC'GCA
Cuts at: 634 2925 634
Size: 2291 2853
HaeII r_GCGC'y
Cuts at: 521 534 720 2578 2826 3848 4827 521
Size: 13 186 1858 248 1022 979 838
Fragments arranged by size:
1858 1022 979 838 248 186 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 548 580 1132 1164 1178 1210 1240 1272 1303
Size: 32 552 32 14 32 30 32 31
Cuts at: 1303 1335 1867 1899 2043 2075 2103 2135 2986
Size: 32 532 32 144 32 28 32 851
Cuts at: 2986 3016 3018 3048 548
Size: 30 2 30 2644
Fragments arranged by size:
2644 851 552 532 144 32 32 32
32 32 32 32 32 31 30 30
30 28 14 2
HincII GTy'rAC
Cuts at: 1859 2183 1859
Size: 324 4820
KasI G'GCGC_C
Cuts at: 2574 2822 2574
Size: 248 4896
KpnI G_GTAC'C
Cuts at: 2164 2164
Size: 5144
MluI A'CGCG_T
Cuts at: 4577 4577
Size: 5144
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 747 3815 3999 747
Size: 3068 184 1892
Fragments arranged by size:
3068 1892 184
MscI TGG'CCA
Cuts at: 170 1829 2905 170
Size: 1659 1076 2409
Fragments arranged by size:
2409 1659 1076
MslI CAynn'nnrTG
Cuts at: 193 295 373 1084 2003 2015 3133 3260 193
Size: 102 78 711 919 12 1118 127 2077
Fragments arranged by size:
2077 1118 919 711 127 102 78 12
MspA1I CmG'CkG
Cuts at: 147 306 552 2255 2569 2929 3942 4187 147
Size: 159 246 1703 314 360 1013 245 1104
Fragments arranged by size:
1703 1104 1013 360 314 246 245 159
NaeI GCC'GGC
Cuts at: 3325 3325
Size: 5144
NarI GG'CG_CC
Cuts at: 2575 2823 2575
Size: 248 4896
NcoI C'CATG_G
Cuts at: 107 968 107
Size: 861 4283
NgoMIV G'CCGG_C
Cuts at: 3323 3323
Size: 5144
NspI r_CATG'y
Cuts at: 242 452 528 2197 2697 3604 242
Size: 210 76 1669 500 907 1782
Fragments arranged by size:
1782 1669 907 500 210 76
PciI A'CATG_T
Cuts at: 3600 3600
Size: 5144
PflMI CCAn_nnn'nTGG
Cuts at: 1290 1462 1290
Size: 172 4972
PmeI GTTT'AAAC
Cuts at: 2143 2143
Size: 5144
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4283 4315 4283
Size: 32 5112
PspOMI G'GGCC_C
Cuts at: 1477 1477
Size: 5144
PstI C_TGCA'G
Cuts at: 699 1423 1473 1530 1875 2191 2876 699
Size: 724 50 57 345 316 685 2967
Fragments arranged by size:
2967 724 685 345 316 57 50
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5144
PvuII CAG'CTG
Cuts at: 147 306 552 2569 2929 147
Size: 159 246 2017 360 2362
Fragments arranged by size:
2362 2017 360 246 159
RsrII CG'GwC_CG
Cuts at: 3339 3339
Size: 5144
SacI G_AGCT'C
Cuts at: 574 625 1114 1759 1938 2158 574
Size: 51 489 645 179 220 3560
Fragments arranged by size:
3560 645 489 220 179 51
SalI G'TCGA_C
Cuts at: 2181 2181
Size: 5144
SapI GCTCTTCn'nnn_
Cuts at: 630 3167 3377 630
Size: 2537 210 2397
Fragments arranged by size:
2537 2397 210
SbfI CC_TGCA'GG
Cuts at: 1473 2191 1473
Size: 718 4426
ScaI AGT'ACT
Cuts at: 4661 4661
Size: 5144
SfcI C'TryA_G
Cuts at: 33 544 695 1419 1469 1526 1871 2187 2872
Size: 511 151 724 50 57 345 316 685
Cuts at: 2872 3865 4056 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 724 685 511 345 316 191
151 57 50
SfoI GGC'GCC
Cuts at: 2576 2824 2576
Size: 248 4896
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5144
SmaI CCC'GGG
Cuts at: 2166 2166
Size: 5144
SmlI C'TyrA_G
Cuts at: 905 2299 3214 3706 3968 905
Size: 1394 915 492 262 2081
Fragments arranged by size:
2081 1394 915 492 262
SpeI A'CTAG_T
Cuts at: 67 997 1411 67
Size: 930 414 3800
Fragments arranged by size:
3800 930 414
SphI G_CATG'C
Cuts at: 242 528 2197 2697 242
Size: 286 1669 500 2689
Fragments arranged by size:
2689 1669 500 286
SspI AAT'ATT
Cuts at: 1398 1398
Size: 5144
StuI AGG'CCT
Cuts at: 334 1548 1900 334
Size: 1214 352 3578
Fragments arranged by size:
3578 1214 352
StyI C'CwwG_G
Cuts at: 107 968 1062 1596 1681 2278 2632 107
Size: 861 94 534 85 597 354 2619
Fragments arranged by size:
2619 861 597 534 354 94 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2727 2860 3319 3528 2727
Size: 133 459 209 4343
Fragments arranged by size:
4343 459 209 133
TatI w'GTAC_w
Cuts at: 4659 4659
Size: 5144
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4661 4693 4661
Size: 32 5112
Tth111I GACn'n_nGTC
Cuts at: 2941 2941
Size: 5144
XbaI T'CTAG_A
Cuts at: 2175 2175
Size: 5144
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 163 375 1635 2639 163
Size: 212 1260 1004 2668
Fragments arranged by size:
2668 1260 1004 212
XmaI C'CCGG_G
Cuts at: 2164 2164
Size: 5144
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PmeI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AlfI AscI AvrII BaeI BbsI
BfrBI BmgBI BmtI BsiWI BsmI BsrGI BssHII BstEII
BstXI ClaI CspCI DraIII FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI
XmnI ZraI