Restriction map for
pF1KA0356
(Circular) (Six-base) MAPSORT of: pF1KA0356.seq Check: 8215 from: 1 to: 6281
pF1KA0356 6281 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1179 1179
Size: 6281
AccI GT'mk_AC
Cuts at: 3071 3319 3071
Size: 248 6033
Acc65I G'GTAC_C
Cuts at: 3297 3297
Size: 6281
AclI AA'CG_TT
Cuts at: 6121 6121
Size: 6281
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 532 770 815 1405 1469 2392 2788 2902 2947
Size: 238 45 590 64 923 396 114 45
Cuts at: 2947 4106 4538 5285 5553 532
Size: 1159 432 747 268 1260
Fragments arranged by size:
1260 1159 923 747 590 432 396 268
238 114 64 45 45
AflIII A'CryG_T
Cuts at: 206 2822 3633 4737 5714 206
Size: 2616 811 1104 977 773
Fragments arranged by size:
2616 1104 977 811 773
AgeI A'CCGG_T
Cuts at: 1016 4669 1016
Size: 3653 2628
AhdI GACnn_n'nnGTC
Cuts at: 2130 2130
Size: 6281
AleI CACnn'nnGTG
Cuts at: 448 1823 1825 448
Size: 1375 2 4904
Fragments arranged by size:
4904 1375 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1171 1171
Size: 6281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 577 609 577
Size: 32 6249
AlwNI CAG_nnn'CTG
Cuts at: 793 846 1201 2198 2550 2766 3586 5153 793
Size: 53 355 997 352 216 820 1567 1921
Fragments arranged by size:
1921 1567 997 820 355 352 216 53
ApaI G_GGCC'C
Cuts at: 2335 2793 2335
Size: 458 5823
ApaLI G'TGCA_C
Cuts at: 3087 5051 3087
Size: 1964 4317
ApoI r'AATT_y
Cuts at: 1042 2512 3052 3285 5634 5890 1042
Size: 1470 540 233 2349 256 1433
Fragments arranged by size:
2349 1470 1433 540 256 233
AseI AT'TA_AT
Cuts at: 20 20
Size: 6281
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6281
AvaI C'yCGr_G
Cuts at: 816 1460 2050 2522 3301 816
Size: 644 590 472 779 3796
Fragments arranged by size:
3796 779 644 590 472
BamHI G'GATC_C
Cuts at: 178 3306 178
Size: 3128 3153
BanI G'GyrC_C
Cuts at: 1167 1245 1723 2055 2920 3297 3711 3959 3994
Size: 78 478 332 865 377 414 248 35
Cuts at: 3994 1167
Size: 3454
Fragments arranged by size:
3454 865 478 414 377 332 248 78
35
BanII G_rGCy'C
Cuts at: 968 1181 1766 1778 2099 2335 2793 2936 3295
Size: 213 585 12 321 236 458 143 359
Cuts at: 3295 4325 968
Size: 1030 2924
Fragments arranged by size:
2924 1030 585 458 359 321 236 213
143 12
BbeI G_GCGC'C
Cuts at: 1249 2059 2924 3715 3963 1249
Size: 810 865 791 248 3567
Fragments arranged by size:
3567 865 810 791 248
BbsI GAAGACnn'nnnn_
Cuts at: 242 363 803 1890 3180 242
Size: 121 440 1087 1290 3343
Fragments arranged by size:
3343 1290 1087 440 121
BbvCI CC'TCA_GC
Cuts at: 314 581 862 1004 1946 1994 314
Size: 267 281 142 942 48 4601
Fragments arranged by size:
4601 942 281 267 142 48
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 6247
BciVI GTATCCnnnnn_n'
Cuts at: 4179 4940 6281 4179
Size: 761 1341 4179
Fragments arranged by size:
4179 1341 761
BclI T'GATC_A
Cuts at: 3801 3801
Size: 6281
BglI GCCn_nnn'nGGC
Cuts at: 271 2330 2461 2918 271
Size: 2059 131 457 3634
Fragments arranged by size:
3634 2059 457 131
BglII A'GATC_T
Cuts at: 3113 3796 5833 3113
Size: 683 2037 3561
Fragments arranged by size:
3561 2037 683
BlpI GC'TnA_GC
Cuts at: 2935 3393 2935
Size: 458 5823
Bme1580I G_kGCm'C
Cuts at: 345 994 1726 2116 2335 2793 3091 3240 3906
Size: 649 732 390 219 458 298 149 666
Cuts at: 3906 3999 5055 345
Size: 93 1056 1571
Fragments arranged by size:
1571 1056 732 666 649 458 390 298
219 149 93
BmgBI CAC'GTC
Cuts at: 623 623
Size: 6281
BmrI ACTGGGnnnn_n'
Cuts at: 2739 3493 3574 3909 6053 2739
Size: 754 81 335 2144 2967
Fragments arranged by size:
2967 2144 754 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 954 1302 1854 4648 954
Size: 348 552 2794 2587
Fragments arranged by size:
2794 2587 552 348
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 1495 1502 1807 2092 289
Size: 1206 7 305 285 4478
Fragments arranged by size:
4478 1206 305 285 7
Bpu10I CC'TnA_GC
Cuts at: 314 581 601 862 1004 1804 1946 1994 314
Size: 267 20 261 142 800 142 48 4601
Fragments arranged by size:
4601 800 267 261 142 142 48 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1193 1538 3022 3457 4336 4828 5126 1193
Size: 345 1484 435 879 492 298 2348
Fragments arranged by size:
2348 1484 879 492 435 345 298
BsaI GGTCTCn'nnnn_
Cuts at: 1025 1540 1714 2287 5985 1025
Size: 515 174 573 3698 1321
Fragments arranged by size:
3698 1321 573 515 174
BsaAI yAC'GTr
Cuts at: 207 1224 3634 207
Size: 1017 2410 2854
Fragments arranged by size:
2854 2410 1017
BsaBI GATnn'nnATC
Cuts at: 6 3795 5832 6
Size: 3789 2037 455
Fragments arranged by size:
3789 2037 455
BsaHI Gr'CG_yC
Cuts at: 1246 2056 2921 3712 3960 4662 6158 1246
Size: 810 865 791 248 702 1496 1369
Fragments arranged by size:
1496 1369 865 810 791 702 248
BsaWI w'CCGG_w
Cuts at: 1016 1673 3470 3991 4669 4943 5090 5501 1016
Size: 657 1797 521 678 274 147 411 1796
Fragments arranged by size:
1797 1796 678 657 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 577 607 955 985 1993 2023 577
Size: 30 348 30 1008 30 4835
Fragments arranged by size:
4835 1008 348 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 598 846 976 1828 1920 2473 2880 598
Size: 248 130 852 92 553 407 3999
Fragments arranged by size:
3999 852 553 407 248 130 92
BseYI C'CCAG_C
Cuts at: 362 887 1249 1719 2792 3062 3092 3706 4464
Size: 525 362 470 1073 270 30 614 758
Cuts at: 4464 5041 5537 362
Size: 577 496 1106
Fragments arranged by size:
1106 1073 758 614 577 525 496 470
362 270 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 279 761 1141 1246 2041 277
Size: 2 482 380 105 795 4517
Fragments arranged by size:
4517 795 482 380 105 2
BsiEI CG_ry'CG
Cuts at: 104 3823 3869 5077 104
Size: 3719 46 1208 1308
Fragments arranged by size:
3719 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 428 1285 1766 1778 1820 2936 3091 3295 4073
Size: 857 481 12 42 1116 155 204 778
Cuts at: 4073 4263 5055 428
Size: 190 792 1654
Fragments arranged by size:
1654 1116 857 792 778 481 204 190
155 42 12
BsmBI CGTCTCn'nnnn_
Cuts at: 2831 2862 2831
Size: 31 6250
Bsp1286I G_dGCh'C
Cuts at: 345 428 968 994 1181 1285 1726 1766 1778
Size: 83 540 26 187 104 441 40 12
Cuts at: 1778 1820 2099 2116 2335 2793 2936 3091 3240
Size: 42 279 17 219 458 143 155 149
Cuts at: 3240 3295 3906 3999 4073 4263 4325 5055 345
Size: 55 611 93 74 190 62 730 1571
Fragments arranged by size:
1571 730 611 540 458 441 279 219
190 187 155 149 143 104 93 83
74 62 55 42 40 26 17 12
BspEI T'CCGG_A
Cuts at: 5501 5501
Size: 6281
BspHI T'CATG_A
Cuts at: 1368 1712 5457 1368
Size: 344 3745 2192
Fragments arranged by size:
3745 2192 344
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1163 1179 2093 2816 3331 3545 3847 4228 1163
Size: 16 914 723 515 214 302 381 3216
Fragments arranged by size:
3216 914 723 515 381 302 214 16
BsrBI CCG'CTC
Cuts at: 50 1734 2847 4573 4627 6111 6276 50
Size: 1684 1113 1726 54 1484 165 55
Fragments arranged by size:
1726 1684 1484 1113 165 55 54
BsrDI GCAATG_nn'
Cuts at: 954 1751 4193 954
Size: 797 2442 3042
Fragments arranged by size:
3042 2442 797
BsrFI r'CCGG_y
Cuts at: 1016 1451 3242 4279 4460 4669 1016
Size: 435 1791 1037 181 209 2628
Fragments arranged by size:
2628 1791 1037 435 209 181
BsrGI T'GTAC_A
Cuts at: 2183 2183
Size: 6281
BssHII G'CGCG_C
Cuts at: 1923 1923
Size: 6281
BssSI C'ACGA_G
Cuts at: 4552 4910 4552
Size: 358 5923
BstAPI GCAn_nnn'nTGC
Cuts at: 3658 3658
Size: 6281
BstBI TT'CG_AA
Cuts at: 635 4642 635
Size: 4007 2274
BstEII G'GTnAC_C
Cuts at: 2466 2466
Size: 6281
BstXI CCAn_nnnn'nTGG
Cuts at: 934 1257 1332 1345 1879 934
Size: 323 75 13 534 5336
Fragments arranged by size:
5336 534 323 75 13
BstYI r'GATC_y
Cuts at: 1 178 1100 1430 2415 2783 3113 3306 3773
Size: 177 922 330 985 368 330 193 467
Cuts at: 3773 3796 4131 4377 5389 5475 5487 5833 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 985 922 467 449 368 346 335
330 330 246 193 177 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1287 2039 1287
Size: 752 5529
BtgI C'CryG_G
Cuts at: 285 1131 1727 2099 2283 2564 285
Size: 846 596 372 184 281 4002
Fragments arranged by size:
4002 846 596 372 281 184
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4231 4412 4575 4231
Size: 181 163 5937
Fragments arranged by size:
5937 181 163
BtsI GCAGTG_nn'
Cuts at: 3075 3524 3075
Size: 449 5832
DraI TTT'AAA
Cuts at: 3280 3280
Size: 6281
DraIII CAC_nnn'GTG
Cuts at: 257 2289 257
Size: 2032 4249
DrdI GACnn_nn'nnGTC
Cuts at: 223 805 1521 3160 3987 4845 223
Size: 582 716 1639 827 858 1659
Fragments arranged by size:
1659 1639 858 827 716 582
EaeI y'GGCC_r
Cuts at: 875 2357 2711 3866 4040 4431 4458 5803 875
Size: 1482 354 1155 174 391 27 1345 1353
Fragments arranged by size:
1482 1353 1345 1155 391 354 174 27
EagI C'GGCC_G
Cuts at: 3866 3866
Size: 6281
EarI CTCTTCn'nnn_
Cuts at: 1771 4304 4514 1771
Size: 2533 210 3538
Fragments arranged by size:
3538 2533 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1783 2124 4489 4799 4945 6091 1783
Size: 341 2365 310 146 1146 1973
Fragments arranged by size:
2365 1973 1146 341 310 146
EcoICRI GAG'CTC
Cuts at: 1764 1776 2934 3293 1764
Size: 12 1158 359 4752
Fragments arranged by size:
4752 1158 359 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 532 770 815 1405 1469 1495 1502 1807
Size: 243 238 45 590 64 26 7 305
Cuts at: 1807 2092 2392 2788 2902 2947 4106 4538 5285
Size: 285 300 396 114 45 1159 432 747
Cuts at: 5285 5553 289
Size: 268 1017
Fragments arranged by size:
1159 1017 747 590 432 396 305 300
285 268 243 238 114 64 45 45
26 7
EcoNI CCTnn'n_nnAGG
Cuts at: 1354 1610 1981 1354
Size: 256 371 5654
Fragments arranged by size:
5654 371 256
EcoO109I rG'GnC_Cy
Cuts at: 615 1974 2195 2331 2733 2763 3042 3420 615
Size: 1359 221 136 402 30 279 378 3476
Fragments arranged by size:
3476 1359 402 378 279 221 136 30
EcoRI G'AATT_C
Cuts at: 1042 3285 1042
Size: 2243 4038
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1782 3204 4300 1782
Size: 1422 1096 3763
Fragments arranged by size:
3763 1422 1096
FspI TGC'GCA
Cuts at: 2601 4062 2601
Size: 1461 4820
HaeII r_GCGC'y
Cuts at: 266 1249 1887 2059 2748 2924 3715 3963 4985
Size: 983 638 172 689 176 791 248 1022
Cuts at: 4985 5964 266
Size: 979 583
Fragments arranged by size:
1022 983 979 791 689 638 583 248
176 172
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 712 744 955 987 2074 2106 2116 2148 2630
Size: 32 211 32 1087 32 10 32 482
Cuts at: 2630 2662 2683 2715 4123 4153 4155 4185 712
Size: 32 21 32 1408 30 2 30 2808
Fragments arranged by size:
2808 1408 1087 482 211 32 32 32
32 32 32 30 30 21 10 2
HincII GTy'rAC
Cuts at: 441 3320 441
Size: 2879 3402
KasI G'GCGC_C
Cuts at: 1245 2055 2920 3711 3959 1245
Size: 810 865 791 248 3567
Fragments arranged by size:
3567 865 810 791 248
KpnI G_GTAC'C
Cuts at: 3301 3301
Size: 6281
MluI A'CGCG_T
Cuts at: 2822 5714 2822
Size: 2892 3389
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 401 1019 1718 3027 4952 5136 401
Size: 618 699 1309 1925 184 1546
Fragments arranged by size:
1925 1546 1309 699 618 184
MscI TGG'CCA
Cuts at: 4042 4042
Size: 6281
MslI CAynn'nnrTG
Cuts at: 448 1823 1825 1877 4270 4397 448
Size: 1375 2 52 2393 127 2332
Fragments arranged by size:
2393 2332 1375 127 52 2
MspA1I CmG'CkG
Cuts at: 458 753 891 1785 2101 2880 3233 3392 3706
Size: 295 138 894 316 779 353 159 314
Cuts at: 3706 4066 5079 5324 458
Size: 360 1013 245 1415
Fragments arranged by size:
1415 1013 894 779 360 353 316 314
295 245 159 138
NaeI GCC'GGC
Cuts at: 3244 4462 3244
Size: 1218 5063
NarI GG'CG_CC
Cuts at: 1246 2056 2921 3712 3960 1246
Size: 810 865 791 248 3567
Fragments arranged by size:
3567 865 810 791 248
NcoI C'CATG_G
Cuts at: 285 1131 2564 285
Size: 846 1433 4002
Fragments arranged by size:
4002 1433 846
NgoMIV G'CCGG_C
Cuts at: 3242 4460 3242
Size: 1218 5063
NspI r_CATG'y
Cuts at: 2842 3070 3334 3834 4741 2842
Size: 228 264 500 907 4382
Fragments arranged by size:
4382 907 500 264 228
PasI CC'CwG_GG
Cuts at: 1578 1644 2061 2472 1578
Size: 66 417 411 5387
Fragments arranged by size:
5387 417 411 66
PciI A'CATG_T
Cuts at: 4737 4737
Size: 6281
PflMI CCAn_nnn'nTGG
Cuts at: 2289 3210 2289
Size: 921 5360
PfoI T'CCnGG_A
Cuts at: 572 2300 572
Size: 1728 4553
PmeI GTTT'AAAC
Cuts at: 3280 3280
Size: 6281
PmlI CAC'GTG
Cuts at: 1224 1224
Size: 6281
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 577 609 5420 5452 577
Size: 32 4811 32 1406
Fragments arranged by size:
4811 1406 32 32
PpuMI rG'GwC_Cy
Cuts at: 2195 2733 2195
Size: 538 5743
PshAI GACnn'nnGTC
Cuts at: 436 436
Size: 6281
PspOMI G'GGCC_C
Cuts at: 2331 2789 2331
Size: 458 5823
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2008 2040 2008
Size: 32 6249
PstI C_TGCA'G
Cuts at: 527 614 901 1176 1355 1982 2381 2546 2758
Size: 87 287 275 179 627 399 165 212
Cuts at: 2758 2813 3328 4013 527
Size: 55 515 685 2795
Fragments arranged by size:
2795 685 627 515 399 287 275 212
179 165 87 55
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6281
PvuII CAG'CTG
Cuts at: 891 2880 3706 4066 891
Size: 1989 826 360 3106
Fragments arranged by size:
3106 1989 826 360
RsrII CG'GwC_CG
Cuts at: 4476 4476
Size: 6281
SacI G_AGCT'C
Cuts at: 1766 1778 2936 3295 1766
Size: 12 1158 359 4752
Fragments arranged by size:
4752 1158 359 12
SacII CC_GC'GG
Cuts at: 2102 2102
Size: 6281
SalI G'TCGA_C
Cuts at: 3318 3318
Size: 6281
SapI GCTCTTCn'nnn_
Cuts at: 1771 4304 4514 1771
Size: 2533 210 3538
Fragments arranged by size:
3538 2533 210
SbfI CC_TGCA'GG
Cuts at: 614 3328 614
Size: 2714 3567
ScaI AGT'ACT
Cuts at: 550 1308 5798 550
Size: 758 4490 1033
Fragments arranged by size:
4490 1033 758
SexAI A'CCwGG_T
Cuts at: 2549 2549
Size: 6281
SfcI C'TryA_G
Cuts at: 33 523 610 748 897 1172 1274 1351 1978
Size: 490 87 138 149 275 102 77 627
Cuts at: 1978 2377 2542 2754 2809 3324 4009 5002 5193
Size: 399 165 212 55 515 685 993 191
Cuts at: 5193 33
Size: 1121
Fragments arranged by size:
1121 993 685 627 515 490 399 275
212 191 165 149 138 102 87 77
55
SfoI GGC'GCC
Cuts at: 1247 2057 2922 3713 3961 1247
Size: 810 865 791 248 3567
Fragments arranged by size:
3567 865 810 791 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6281
SgrAI Cr'CCGG_yG
Cuts at: 3242 3242
Size: 6281
SmaI CCC'GGG
Cuts at: 1462 2524 3303 1462
Size: 1062 779 4440
Fragments arranged by size:
4440 1062 779
SmlI C'TyrA_G
Cuts at: 1208 1553 3037 3436 4351 4843 5105 1208
Size: 345 1484 399 915 492 262 2384
Fragments arranged by size:
2384 1484 915 492 399 345 262
SpeI A'CTAG_T
Cuts at: 67 229 67
Size: 162 6119
SphI G_CATG'C
Cuts at: 3334 3834 3334
Size: 500 5781
StuI AGG'CCT
Cuts at: 190 1117 1530 2576 190
Size: 927 413 1046 3895
Fragments arranged by size:
3895 1046 927 413
StyI C'CwwG_G
Cuts at: 285 1131 1338 2225 2325 2360 2564 2586 3415
Size: 846 207 887 100 35 204 22 829
Cuts at: 3415 3769 285
Size: 354 2797
Fragments arranged by size:
2797 887 846 829 354 207 204 100
35 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 412 1419 1508 2001 3106 3864 3997 4456 4665
Size: 1007 89 493 1105 758 133 459 209
Cuts at: 4665 412
Size: 2028
Fragments arranged by size:
2028 1105 1007 758 493 459 209 133
89
TatI w'GTAC_w
Cuts at: 548 1306 2177 2183 5796 548
Size: 758 871 6 3613 1033
Fragments arranged by size:
3613 1033 871 758 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 324 356 1993 2025 5798 5830 324
Size: 32 1637 32 3773 32 775
Fragments arranged by size:
3773 1637 775 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4078 4078
Size: 6281
XbaI T'CTAG_A
Cuts at: 3312 3312
Size: 6281
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1198 3776 114
Size: 1084 2578 2619
Fragments arranged by size:
2619 2578 1084
XmaI C'CCGG_G
Cuts at: 1460 2522 3301 1460
Size: 1062 779 4440
Fragments arranged by size:
4440 1062 779
XmnI GAAnn'nnTTC
Cuts at: 2242 2533 3268 2242
Size: 291 735 5255
Fragments arranged by size:
5255 735 291
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AscI AvrII BaeI BfrBI BmtI
BsiWI BsmI BstZ17I ClaI CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PsiI PspXI SanDI SfiI SnaBI SrfI SspI
SwaI XhoI ZraI