Restriction map for
pF1KA0361
(Circular) (Six-base) MAPSORT of: pF1KA0361.seq Check: 8950 from: 1 to: 7127
pF1KA0361 7127 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1506 1506
Size: 7127
AccI GT'mk_AC
Cuts at: 1475 4165 1475
Size: 2690 4437
Acc65I G'GTAC_C
Cuts at: 4143 4143
Size: 7127
AclI AA'CG_TT
Cuts at: 1854 6967 1854
Size: 5113 2014
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2663 2766 3022 3441 3549 4952 5384 6131 6399
Size: 103 256 419 108 1403 432 747 268
Cuts at: 6399 2663
Size: 3391
Fragments arranged by size:
3391 1403 747 432 419 268 256 108
103
AfeI AGC'GCT
Cuts at: 724 2140 724
Size: 1416 5711
AflIII A'CryG_T
Cuts at: 1014 4479 5583 6560 1014
Size: 3465 1104 977 1581
Fragments arranged by size:
3465 1581 1104 977
AgeI A'CCGG_T
Cuts at: 5515 5515
Size: 7127
AhdI GACnn_n'nnGTC
Cuts at: 1018 1018
Size: 7127
AlwNI CAG_nnn'CTG
Cuts at: 163 843 2064 2100 2617 2799 3000 3502 4432
Size: 680 1221 36 517 182 201 502 930
Cuts at: 4432 5999 163
Size: 1567 1291
Fragments arranged by size:
1567 1291 1221 930 680 517 502 201
182 36
ApaI G_GGCC'C
Cuts at: 383 1131 2207 3065 3230 3655 383
Size: 748 1076 858 165 425 3855
Fragments arranged by size:
3855 1076 858 748 425 165
ApaLI G'TGCA_C
Cuts at: 847 1113 2089 5897 847
Size: 266 976 3808 2077
Fragments arranged by size:
3808 2077 976 266
ApoI r'AATT_y
Cuts at: 910 1227 4131 6480 6736 910
Size: 317 2904 2349 256 1301
Fragments arranged by size:
2904 2349 1301 317 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7127
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7127
AvaI C'yCGr_G
Cuts at: 329 747 2537 2695 3296 4147 329
Size: 418 1790 158 601 851 3309
Fragments arranged by size:
3309 1790 851 601 418 158
BamHI G'GATC_C
Cuts at: 4152 4152
Size: 7127
BanI G'GyrC_C
Cuts at: 1876 3778 4143 4557 4805 4840 1876
Size: 1902 365 414 248 35 4163
Fragments arranged by size:
4163 1902 414 365 248 35
BanII G_rGCy'C
Cuts at: 383 1131 1994 2207 2261 2565 2680 3065 3135
Size: 748 863 213 54 304 115 385 70
Cuts at: 3135 3230 3481 3655 4141 5171 383
Size: 95 251 174 486 1030 2339
Fragments arranged by size:
2339 1030 863 748 486 385 304 251
213 174 115 95 70 54
BbeI G_GCGC'C
Cuts at: 3782 4561 4809 3782
Size: 779 248 6100
Fragments arranged by size:
6100 779 248
BbsI GAAGACnn'nnnn_
Cuts at: 1014 1014
Size: 7127
BbvCI CC'TCA_GC
Cuts at: 251 269 512 1807 2524 2948 3535 251
Size: 18 243 1295 717 424 587 3843
Fragments arranged by size:
3843 1295 717 587 424 243 18
BciVI GTATCCnnnnn_n'
Cuts at: 1229 5025 5786 7127 1229
Size: 3796 761 1341 1229
Fragments arranged by size:
3796 1341 1229 761
BclI T'GATC_A
Cuts at: 1601 1653 4647 1601
Size: 52 2994 4081
Fragments arranged by size:
4081 2994 52
BglI GCCn_nnn'nGGC
Cuts at: 2079 2332 2464 3050 3672 2079
Size: 253 132 586 622 5534
Fragments arranged by size:
5534 622 586 253 132
BglII A'GATC_T
Cuts at: 4642 6679 4642
Size: 2037 5090
BlpI GC'TnA_GC
Cuts at: 2083 3530 4239 2083
Size: 1447 709 4971
Fragments arranged by size:
4971 1447 709
Bme1580I G_kGCm'C
Cuts at: 383 851 1117 1131 1848 1879 2093 2207 2704
Size: 468 266 14 717 31 214 114 497
Cuts at: 2704 3065 3230 3272 3655 4752 4845 5901 383
Size: 361 165 42 383 1097 93 1056 1609
Fragments arranged by size:
1609 1097 1056 717 497 468 383 361
266 214 165 114 93 42 31 14
BmgBI CAC'GTC
Cuts at: 3384 3384
Size: 7127
BmrI ACTGGGnnnn_n'
Cuts at: 109 257 449 646 2709 3891 4339 4420 4755
Size: 148 192 197 2063 1182 448 81 335
Cuts at: 4755 6899 109
Size: 2144 337
Fragments arranged by size:
2144 2063 1182 448 337 335 197 192
148 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5494 5494
Size: 7127
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 679 1264 1321 1727 1907 2008 2122 2729
Size: 288 585 57 406 180 101 114 607
Cuts at: 2729 2887 2989 3055 3127 4060 391
Size: 158 102 66 72 933 3458
Fragments arranged by size:
3458 933 607 585 406 288 180 158
114 102 101 72 66 57
Bpu10I CC'TnA_GC
Cuts at: 251 269 512 1807 2524 2823 2948 3535 4001
Size: 18 243 1295 717 299 125 587 466
Cuts at: 4001 251
Size: 3377
Fragments arranged by size:
3377 1295 717 587 466 299 243 125
18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2617 4303 5182 5674 5972 2617
Size: 1686 879 492 298 3772
Fragments arranged by size:
3772 1686 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 460 1562 1883 2545 3326 6831 460
Size: 1102 321 662 781 3505 756
Fragments arranged by size:
3505 1102 781 756 662 321
BsaAI yAC'GTr
Cuts at: 828 1134 3819 4480 828
Size: 306 2685 661 3475
Fragments arranged by size:
3475 2685 661 306
BsaBI GATnn'nnATC
Cuts at: 6 4641 6678 6
Size: 4635 2037 455
Fragments arranged by size:
4635 2037 455
BsaHI Gr'CG_yC
Cuts at: 212 3779 4558 4806 5508 7004 212
Size: 3567 779 248 702 1496 335
Fragments arranged by size:
3567 1496 779 702 335 248
BsaWI w'CCGG_w
Cuts at: 548 1892 3545 3834 4316 4837 5515 5789 5936
Size: 1344 1653 289 482 521 678 274 147
Cuts at: 5936 6347 548
Size: 411 1328
Fragments arranged by size:
1653 1344 1328 678 521 482 411 289
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1457 1487 2165 2195 2837 2867 1457
Size: 30 678 30 642 30 5717
Fragments arranged by size:
5717 678 642 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 286 1580 2207 2269 2806 2965 3124 3157 3211
Size: 1294 627 62 537 159 159 33 54
Cuts at: 3211 3331 286
Size: 120 4082
Fragments arranged by size:
4082 1294 627 537 159 159 120 62
54 33
BseYI C'CCAG_C
Cuts at: 444 1701 2004 2186 2726 3039 3229 3289 3364
Size: 1257 303 182 540 313 190 60 75
Cuts at: 3364 3523 3611 3662 3722 4552 5310 5887 6383
Size: 159 88 51 60 830 758 577 496
Cuts at: 6383 444
Size: 1188
Fragments arranged by size:
1257 1188 830 758 577 540 496 313
303 190 182 159 88 75 60 60
51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 526 1534 1540 1579 2995 3862 526
Size: 1008 6 39 1416 867 3791
Fragments arranged by size:
3791 1416 1008 867 39 6
BsiEI CG_ry'CG
Cuts at: 104 549 1917 4669 4715 5923 104
Size: 445 1368 2752 46 1208 1308
Fragments arranged by size:
2752 1368 1308 1208 445 46
BsiHKAI G_wGCw'C
Cuts at: 754 794 851 877 1117 1994 2093 2261 4141
Size: 40 57 26 240 877 99 168 1880
Cuts at: 4141 4919 5109 5901 754
Size: 778 190 792 1980
Fragments arranged by size:
1980 1880 877 792 778 240 190 168
99 57 40 26
BsmI GAATG_Cn'
Cuts at: 1761 1761
Size: 7127
BsmBI CGTCTCn'nnnn_
Cuts at: 3139 3139
Size: 7127
Bsp1286I G_dGCh'C
Cuts at: 383 754 794 851 877 1117 1131 1848 1879
Size: 371 40 57 26 240 14 717 31
Cuts at: 1879 1994 2093 2207 2261 2565 2680 2704 3065
Size: 115 99 114 54 304 115 24 361
Cuts at: 3065 3135 3230 3272 3481 3655 4141 4752 4845
Size: 70 95 42 209 174 486 611 93
Cuts at: 4845 4919 5109 5171 5901 383
Size: 74 190 62 730 1609
Fragments arranged by size:
1609 730 717 611 486 371 361 304
240 209 190 174 115 115 114 99
95 93 74 70 62 57 54 42
40 31 26 24 14
BspEI T'CCGG_A
Cuts at: 3545 3834 6347 3545
Size: 289 2513 4325
Fragments arranged by size:
4325 2513 289
BspHI T'CATG_A
Cuts at: 869 1650 6303 869
Size: 781 4653 1693
Fragments arranged by size:
4653 1693 781
BspMI ACCTGCnnnn'nnnn_
Cuts at: 371 1506 4177 4391 4693 5074 371
Size: 1135 2671 214 302 381 2424
Fragments arranged by size:
2671 2424 1135 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1325 2165 3219 4005 5419 5473 6957 7122
Size: 1275 840 1054 786 1414 54 1484 165
Cuts at: 7122 50
Size: 55
Fragments arranged by size:
1484 1414 1275 1054 840 786 165 55
54
BsrDI GCAATG_nn'
Cuts at: 524 1677 1683 3027 5039 524
Size: 1153 6 1344 2012 2612
Fragments arranged by size:
2612 2012 1344 1153 6
BsrFI r'CCGG_y
Cuts at: 482 647 1150 2690 3164 5125 5306 5515 482
Size: 165 503 1540 474 1961 181 209 2094
Fragments arranged by size:
2094 1961 1540 503 474 209 181 165
BssSI C'ACGA_G
Cuts at: 2962 5398 5756 2962
Size: 2436 358 4333
Fragments arranged by size:
4333 2436 358
BstAPI GCAn_nnn'nTGC
Cuts at: 4504 4504
Size: 7127
BstBI TT'CG_AA
Cuts at: 5488 5488
Size: 7127
BstXI CCAn_nnnn'nTGG
Cuts at: 444 531 679 832 1222 1542 2157 2280 3364
Size: 87 148 153 390 320 615 123 1084
Cuts at: 3364 3867 3881 444
Size: 503 14 3690
Fragments arranged by size:
3690 1084 615 503 390 320 153 148
123 87 14
BstYI r'GATC_y
Cuts at: 1 1203 4152 4619 4642 4977 5223 6235 6321
Size: 1202 2949 467 23 335 246 1012 86
Cuts at: 6321 6333 6679 1
Size: 12 346 449
Fragments arranged by size:
2949 1202 1012 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 965 3211 965
Size: 2246 4881
BtgI C'CryG_G
Cuts at: 1122 1403 2126 2168 2336 3071 3432 1122
Size: 281 723 42 168 735 361 4817
Fragments arranged by size:
4817 735 723 361 281 168 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5077 5258 5421 5077
Size: 181 163 6783
Fragments arranged by size:
6783 181 163
BtsI GCAGTG_nn'
Cuts at: 933 1145 2200 2483 2507 3874 4030 4370 933
Size: 212 1055 283 24 1367 156 340 3690
Fragments arranged by size:
3690 1367 1055 340 283 212 156 24
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1961 1996 1961
Size: 35 7092
DraI TTT'AAA
Cuts at: 4126 4126
Size: 7127
DraIII CAC_nnn'GTG
Cuts at: 1137 1137
Size: 7127
DrdI GACnn_nn'nnGTC
Cuts at: 2317 4833 5691 2317
Size: 2516 858 3753
Fragments arranged by size:
3753 2516 858
EaeI y'GGCC_r
Cuts at: 144 979 1350 1941 2129 2333 2339 3344 3553
Size: 835 371 591 188 204 6 1005 209
Cuts at: 3553 3673 3972 4712 4886 5277 5304 6649 144
Size: 120 299 740 174 391 27 1345 622
Fragments arranged by size:
1345 1005 835 740 622 591 391 371
299 209 204 188 174 120 27 6
EagI C'GGCC_G
Cuts at: 4712 4712
Size: 7127
EarI CTCTTCn'nnn_
Cuts at: 1805 5150 5360 1805
Size: 3345 210 3572
Fragments arranged by size:
3572 3345 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2467 5335 5645 5791 6937 2467
Size: 2868 310 146 1146 2657
Fragments arranged by size:
2868 2657 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1992 2259 4139 1992
Size: 267 1880 4980
Fragments arranged by size:
4980 1880 267
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 679 1264 1321 1727 1907 2008 2122 2663
Size: 288 585 57 406 180 101 114 541
Cuts at: 2663 2729 2766 2887 2989 3022 3055 3127 3441
Size: 66 37 121 102 33 33 72 314
Cuts at: 3441 3549 4060 4952 5384 6131 6399 391
Size: 108 511 892 432 747 268 1119
Fragments arranged by size:
1119 892 747 585 541 511 432 406
314 288 268 180 121 114 108 102
101 72 66 57 37 33 33
EcoNI CCTnn'n_nnAGG
Cuts at: 2992 3139 2992
Size: 147 6980
EcoO109I rG'GnC_Cy
Cuts at: 255 448 702 969 1127 1431 1468 1620 1743
Size: 193 254 267 158 304 37 152 123
Cuts at: 1743 1811 2203 2204 3062 3207 3226 3396 3652
Size: 68 392 1 858 145 19 170 256
Cuts at: 3652 3742 3935 4266 255
Size: 90 193 331 3116
Fragments arranged by size:
3116 858 392 331 304 267 256 254
193 193 170 158 152 145 123 90
68 37 19 1
EcoRI G'AATT_C
Cuts at: 4131 4131
Size: 7127
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2046 5146 2046
Size: 3100 4027
FspI TGC'GCA
Cuts at: 3803 4908 3803
Size: 1105 6022
FspAI rTGC'GCAy
Cuts at: 3803 3803
Size: 7127
HaeII r_GCGC'y
Cuts at: 480 726 1664 2142 3543 3711 3782 4561 4809
Size: 246 938 478 1401 168 71 779 248
Cuts at: 4809 5831 6810 480
Size: 1022 979 797
Fragments arranged by size:
1401 1022 979 938 797 779 478 248
246 168 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1004 1036 1457 1489 2640 2672 2837 2869 2917
Size: 32 421 32 1151 32 165 32 48
Cuts at: 2917 2949 3290 3322 3388 3420 3498 3530 4969
Size: 32 341 32 66 32 78 32 1439
Cuts at: 4969 4999 5001 5031 1004
Size: 30 2 30 3100
Fragments arranged by size:
3100 1439 1151 421 341 165 78 66
48 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 3592 4166 3592
Size: 574 6553
HindIII A'AGCT_T
Cuts at: 1209 1209
Size: 7127
KasI G'GCGC_C
Cuts at: 3778 4557 4805 3778
Size: 779 248 6100
Fragments arranged by size:
6100 779 248
KpnI G_GTAC'C
Cuts at: 4147 4147
Size: 7127
MfeI C'AATT_G
Cuts at: 3413 3413
Size: 7127
MluI A'CGCG_T
Cuts at: 6560 6560
Size: 7127
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1443 1470 5798 5982 1443
Size: 27 4328 184 2588
Fragments arranged by size:
4328 2588 184 27
MscI TGG'CCA
Cuts at: 146 1943 2131 4888 146
Size: 1797 188 2757 2385
Fragments arranged by size:
2757 2385 1797 188
MslI CAynn'nnrTG
Cuts at: 128 830 1841 5116 5243 128
Size: 702 1011 3275 127 2012
Fragments arranged by size:
3275 2012 1011 702 127
MspA1I CmG'CkG
Cuts at: 250 511 741 1124 1356 1705 3159 3499 4238
Size: 261 230 383 232 349 1454 340 739
Cuts at: 4238 4552 4912 5925 6170 250
Size: 314 360 1013 245 1207
Fragments arranged by size:
1454 1207 1013 739 383 360 349 340
314 261 245 232 230
NaeI GCC'GGC
Cuts at: 649 1152 5308 649
Size: 503 4156 2468
Fragments arranged by size:
4156 2468 503
NarI GG'CG_CC
Cuts at: 3779 4558 4806 3779
Size: 779 248 6100
Fragments arranged by size:
6100 779 248
NcoI C'CATG_G
Cuts at: 1403 3071 1403
Size: 1668 5459
NgoMIV G'CCGG_C
Cuts at: 647 1150 5306 647
Size: 503 4156 2468
Fragments arranged by size:
4156 2468 503
NspI r_CATG'y
Cuts at: 606 1018 2861 4180 4680 5587 606
Size: 412 1843 1319 500 907 2146
Fragments arranged by size:
2146 1843 1319 907 500 412
PasI CC'CwG_GG
Cuts at: 1196 2077 2431 2490 2680 2918 3518 3940 1196
Size: 881 354 59 190 238 600 422 4383
Fragments arranged by size:
4383 881 600 422 354 238 190 59
PciI A'CATG_T
Cuts at: 1014 5583 1014
Size: 4569 2558
PflMI CCAn_nnn'nTGG
Cuts at: 1137 2100 3033 3072 3491 1137
Size: 963 933 39 419 4773
Fragments arranged by size:
4773 963 933 419 39
PfoI T'CCnGG_A
Cuts at: 728 2384 728
Size: 1656 5471
PmeI GTTT'AAAC
Cuts at: 4126 4126
Size: 7127
PmlI CAC'GTG
Cuts at: 828 1134 828
Size: 306 6821
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6266 6298 6266
Size: 32 7095
PpuMI rG'GwC_Cy
Cuts at: 702 969 1468 1743 1811 3396 3935 702
Size: 267 499 275 68 1585 539 3894
Fragments arranged by size:
3894 1585 539 499 275 267 68
PshAI GACnn'nnGTC
Cuts at: 1837 1837
Size: 7127
PspOMI G'GGCC_C
Cuts at: 379 1127 2203 3061 3226 3651 379
Size: 748 1076 858 165 425 3855
Fragments arranged by size:
3855 1076 858 748 425 165
PstI C_TGCA'G
Cuts at: 209 1645 2039 2060 3645 4174 4859 209
Size: 1436 394 21 1585 529 685 2477
Fragments arranged by size:
2477 1585 1436 685 529 394 21
PvuI CG_AT'CG
Cuts at: 104 1917 104
Size: 1813 5314
PvuII CAG'CTG
Cuts at: 250 511 1705 3159 3499 4552 4912 250
Size: 261 1194 1454 340 1053 360 2465
Fragments arranged by size:
2465 1454 1194 1053 360 340 261
RsrII CG'GwC_CG
Cuts at: 5322 5322
Size: 7127
SacI G_AGCT'C
Cuts at: 1994 2261 4141 1994
Size: 267 1880 4980
Fragments arranged by size:
4980 1880 267
SacII CC_GC'GG
Cuts at: 1125 1125
Size: 7127
SalI G'TCGA_C
Cuts at: 4164 4164
Size: 7127
SanDI GG'GwC_CC
Cuts at: 1743 3935 1743
Size: 2192 4935
SapI GCTCTTCn'nnn_
Cuts at: 1805 5150 5360 1805
Size: 3345 210 3572
Fragments arranged by size:
3572 3345 210
SbfI CC_TGCA'GG
Cuts at: 4174 4174
Size: 7127
ScaI AGT'ACT
Cuts at: 6644 6644
Size: 7127
SexAI A'CCwGG_T
Cuts at: 2357 2357
Size: 7127
SfcI C'TryA_G
Cuts at: 33 205 736 1476 1641 2035 2056 2896 3169
Size: 172 531 740 165 394 21 840 273
Cuts at: 3169 3641 4170 4855 5848 6039 33
Size: 472 529 685 993 191 1121
Fragments arranged by size:
1121 993 840 740 685 531 529 472
394 273 191 172 165 21
SfoI GGC'GCC
Cuts at: 3780 4559 4807 3780
Size: 779 248 6100
Fragments arranged by size:
6100 779 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7127
SmaI CCC'GGG
Cuts at: 4149 4149
Size: 7127
SmlI C'TyrA_G
Cuts at: 2632 4282 5197 5689 5951 2632
Size: 1650 915 492 262 3808
Fragments arranged by size:
3808 1650 915 492 262
SnaBI TAC'GTA
Cuts at: 3819 3819
Size: 7127
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7127
SphI G_CATG'C
Cuts at: 606 4180 4680 606
Size: 3574 500 3053
Fragments arranged by size:
3574 3053 500
StuI AGG'CCT
Cuts at: 763 2177 2849 2960 3680 4017 763
Size: 1414 672 111 720 337 3873
Fragments arranged by size:
3873 1414 720 672 337 111
StyI C'CwwG_G
Cuts at: 1328 1403 1625 2279 3071 4018 4261 4615 1328
Size: 75 222 654 792 947 243 354 3840
Fragments arranged by size:
3840 947 792 654 354 243 222 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 628 892 1989 2482 3603 4710 4843 5302 5511
Size: 264 1097 493 1121 1107 133 459 209
Cuts at: 5511 628
Size: 2244
Fragments arranged by size:
2244 1121 1107 1097 493 459 264 209
133
TatI w'GTAC_w
Cuts at: 6642 6642
Size: 7127
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2165 2197 2380 2412 6644 6676 2165
Size: 32 183 32 4232 32 2616
Fragments arranged by size:
4232 2616 183 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4924 4924
Size: 7127
XbaI T'CTAG_A
Cuts at: 4158 4158
Size: 7127
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1138 3700 3735 4622 1138
Size: 2562 35 887 3643
Fragments arranged by size:
3643 2562 887 35
XmaI C'CCGG_G
Cuts at: 4147 4147
Size: 7127
XmnI GAAnn'nnTTC
Cuts at: 2026 2026
Size: 7127
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AleI AlfI AloI AscI AvrII BaeI
BcgI BfrBI BmtI BsiWI BsrGI BssHII BstEII BstZ17I
ClaI EcoRV FseI HpaI NdeI NheI NotI NruI
NsiI PacI PsiI PspXI PsrI SfiI SgrAI SrfI
SspI SwaI XhoI ZraI