Restriction map for
pF1KA0365
(Circular) (Six-base) MAPSORT of: pF1KA0365.seq Check: 8872 from: 1 to: 6359
pF1KA0365 6359 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1647 2925 1647
Size: 1278 5081
AatII G_ACGT'C
Cuts at: 278 278
Size: 6359
AccI GT'mk_AC
Cuts at: 3397 3397
Size: 6359
Acc65I G'GTAC_C
Cuts at: 2941 3375 2941
Size: 434 5925
AclI AA'CG_TT
Cuts at: 6199 6199
Size: 6359
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 360 1039 1438 1720 2023 2426 4184 4616
Size: 164 679 399 282 303 403 1758 432
Cuts at: 4616 5363 5631 196
Size: 747 268 924
Fragments arranged by size:
1758 924 747 679 432 403 399 303
282 268 164
AflII C'TTAA_G
Cuts at: 234 234
Size: 6359
AflIII A'CryG_T
Cuts at: 1893 3711 4815 5792 1893
Size: 1818 1104 977 2460
Fragments arranged by size:
2460 1818 1104 977
AgeI A'CCGG_T
Cuts at: 4747 4747
Size: 6359
AleI CACnn'nnGTG
Cuts at: 2663 2663
Size: 6359
AlwNI CAG_nnn'CTG
Cuts at: 807 3132 3162 3664 5231 807
Size: 2325 30 502 1567 1935
Fragments arranged by size:
2325 1935 1567 502 30
ApaLI G'TGCA_C
Cuts at: 5129 5129
Size: 6359
ApoI r'AATT_y
Cuts at: 442 517 1144 1264 1802 2590 3363 5712 5968
Size: 75 627 120 538 788 773 2349 256
Cuts at: 5968 442
Size: 833
Fragments arranged by size:
2349 833 788 773 627 538 256 120
75
AseI AT'TA_AT
Cuts at: 20 20
Size: 6359
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6359
AvaI C'yCGr_G
Cuts at: 461 2099 3071 3379 461
Size: 1638 972 308 3441
Fragments arranged by size:
3441 1638 972 308
AvrII C'CTAG_G
Cuts at: 783 783
Size: 6359
BamHI G'GATC_C
Cuts at: 3384 3384
Size: 6359
BanI G'GyrC_C
Cuts at: 1870 2227 2941 3375 3789 4037 4072 1870
Size: 357 714 434 414 248 35 4157
Fragments arranged by size:
4157 714 434 414 357 248 35
BanII G_rGCy'C
Cuts at: 753 2153 2171 2698 2741 2770 3202 3373 4403
Size: 1400 18 527 43 29 432 171 1030
Cuts at: 4403 753
Size: 2709
Fragments arranged by size:
2709 1400 1030 527 432 171 43 29
18
BbeI G_GCGC'C
Cuts at: 3793 4041 3793
Size: 248 6111
BbsI GAAGACnn'nnnn_
Cuts at: 1351 1483 1667 2461 2738 3296 1351
Size: 132 184 794 277 558 4414
Fragments arranged by size:
4414 794 558 277 184 132
BbvCI CC'TCA_GC
Cuts at: 2064 2064
Size: 6359
BciVI GTATCCnnnnn_n'
Cuts at: 4257 5018 6359 4257
Size: 761 1341 4257
Fragments arranged by size:
4257 1341 761
BclI T'GATC_A
Cuts at: 3879 3879
Size: 6359
BglI GCCn_nnn'nGGC
Cuts at: 2882 2882
Size: 6359
BglII A'GATC_T
Cuts at: 228 3874 5911 228
Size: 3646 2037 676
Fragments arranged by size:
3646 2037 676
BlpI GC'TnA_GC
Cuts at: 2756 3471 2756
Size: 715 5644
Bme1580I G_kGCm'C
Cuts at: 910 1873 3984 4077 5133 910
Size: 963 2111 93 1056 2136
Fragments arranged by size:
2136 2111 1056 963 93
BmgBI CAC'GTC
Cuts at: 684 2639 684
Size: 1955 4404
BmrI ACTGGGnnnn_n'
Cuts at: 2323 3571 3652 3987 6131 2323
Size: 1248 81 335 2144 2551
Fragments arranged by size:
2551 2144 1248 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 723 3034 4726 723
Size: 2311 1692 2356
Fragments arranged by size:
2356 2311 1692
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1717 2002 2243 2782 2855 2930 1717
Size: 285 241 539 73 75 5146
Fragments arranged by size:
5146 539 285 241 75 73
Bpu10I CC'TnA_GC
Cuts at: 715 818 2064 2495 715
Size: 103 1246 431 4579
Fragments arranged by size:
4579 1246 431 103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 209 551 2101 2147 3535 4414 4906 5204 209
Size: 342 1550 46 1388 879 492 298 1364
Fragments arranged by size:
1550 1388 1364 879 492 342 298 46
BsaI GGTCTCn'nnnn_
Cuts at: 6063 6063
Size: 6359
BsaAI yAC'GTr
Cuts at: 3712 3712
Size: 6359
BsaBI GATnn'nnATC
Cuts at: 6 3873 5910 6
Size: 3867 2037 455
Fragments arranged by size:
3867 2037 455
BsaHI Gr'CG_yC
Cuts at: 275 3790 4038 4740 6236 275
Size: 3515 248 702 1496 398
Fragments arranged by size:
3515 1496 702 398 248
BsaWI w'CCGG_w
Cuts at: 482 3548 4069 4747 5021 5168 5579 482
Size: 3066 521 678 274 147 411 1262
Fragments arranged by size:
3066 1262 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2409 2439 2628 2658 2409
Size: 30 189 30 6110
Fragments arranged by size:
6110 189 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1783 1786 2057 2143 2202 2333 2800 2803 2833
Size: 3 271 86 59 131 467 3 30
Cuts at: 2833 2925 1783
Size: 92 5217
Fragments arranged by size:
5217 467 271 131 92 86 59 30
3 3
BseYI C'CCAG_C
Cuts at: 1844 2353 3122 3784 4542 5119 5615 1844
Size: 509 769 662 758 577 496 2588
Fragments arranged by size:
2588 769 758 662 577 509 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2096 2096
Size: 6359
BsiEI CG_ry'CG
Cuts at: 104 2250 3901 3947 5155 104
Size: 2146 1651 46 1208 1308
Fragments arranged by size:
2146 1651 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 753 862 1238 2163 2916 3284 3373 4151 4341
Size: 109 376 925 753 368 89 778 190
Cuts at: 4341 5133 753
Size: 792 1979
Fragments arranged by size:
1979 925 792 778 753 376 368 190
109 89
Bsp1286I G_dGCh'C
Cuts at: 753 862 910 1238 1873 2153 2163 2171 2698
Size: 109 48 328 635 280 10 8 527
Cuts at: 2698 2741 2770 2916 3202 3284 3373 3984 4077
Size: 43 29 146 286 82 89 611 93
Cuts at: 4077 4151 4341 4403 5133 753
Size: 74 190 62 730 1979
Fragments arranged by size:
1979 730 635 611 527 328 286 280
190 146 109 93 89 82 74 62
48 43 29 10 8
BspEI T'CCGG_A
Cuts at: 5579 5579
Size: 6359
BspHI T'CATG_A
Cuts at: 5535 5535
Size: 6359
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1647 2925 2953 3409 3623 3925 4306 1647
Size: 1278 28 456 214 302 381 3700
Fragments arranged by size:
3700 1278 456 381 302 214 28
BsrBI CCG'CTC
Cuts at: 50 2830 4651 4705 6189 6354 50
Size: 2780 1821 54 1484 165 55
Fragments arranged by size:
2780 1821 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1455 4271 1455
Size: 2816 3543
BsrFI r'CCGG_y
Cuts at: 1409 2249 3225 4357 4538 4747 1409
Size: 840 976 1132 181 209 3021
Fragments arranged by size:
3021 1132 976 840 209 181
BsrGI T'GTAC_A
Cuts at: 3329 3329
Size: 6359
BssSI C'ACGA_G
Cuts at: 4630 4988 4630
Size: 358 6001
BstAPI GCAn_nnn'nTGC
Cuts at: 2074 3736 2074
Size: 1662 4697
BstBI TT'CG_AA
Cuts at: 1658 3051 4720 1658
Size: 1393 1669 3297
Fragments arranged by size:
3297 1669 1393
BstXI CCAn_nnnn'nTGG
Cuts at: 286 475 3111 286
Size: 189 2636 3534
Fragments arranged by size:
3534 2636 189
BstYI r'GATC_y
Cuts at: 1 228 250 307 435 628 998 1014 3384
Size: 227 22 57 128 193 370 16 2370
Cuts at: 3384 3851 3874 4209 4455 5467 5553 5565 5911
Size: 467 23 335 246 1012 86 12 346
Cuts at: 5911 1
Size: 449
Fragments arranged by size:
2370 1012 467 449 370 346 335 246
227 193 128 86 57 23 22 16
12
Bsu36I CC'TnA_GG
Cuts at: 2223 2345 2367 2783 2937 2223
Size: 122 22 416 154 5645
Fragments arranged by size:
5645 416 154 122 22
BtgI C'CryG_G
Cuts at: 107 528 2134 2244 2678 2699 2746 3189 107
Size: 421 1606 110 434 21 47 443 3277
Fragments arranged by size:
3277 1606 443 434 421 110 47 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 299 4309 4490 4653 299
Size: 4010 181 163 2005
Fragments arranged by size:
4010 2005 181 163
BtsI GCAGTG_nn'
Cuts at: 2084 3602 2084
Size: 1518 4841
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 408 443 1656 1691 408
Size: 35 1213 35 5076
Fragments arranged by size:
5076 1213 35 35
DraI TTT'AAA
Cuts at: 219 1271 1316 3358 219
Size: 1052 45 2042 3220
Fragments arranged by size:
3220 2042 1052 45
DrdI GACnn_nn'nnGTC
Cuts at: 4065 4923 4065
Size: 858 5501
EaeI y'GGCC_r
Cuts at: 429 477 552 2247 3944 4118 4509 4536 5881
Size: 48 75 1695 1697 174 391 27 1345
Cuts at: 5881 429
Size: 907
Fragments arranged by size:
1697 1695 1345 907 391 174 75 48
27
EagI C'GGCC_G
Cuts at: 2247 3944 2247
Size: 1697 4662
EarI CTCTTCn'nnn_
Cuts at: 215 330 1414 1584 1937 2427 2953 4382 4592
Size: 115 1084 170 353 490 526 1429 210
Cuts at: 4592 215
Size: 1982
Fragments arranged by size:
1982 1429 1084 526 490 353 210 170
115
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4567 4877 5023 6169 4567
Size: 310 146 1146 4757
Fragments arranged by size:
4757 1146 310 146
EcoICRI GAG'CTC
Cuts at: 751 3371 751
Size: 2620 3739
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 360 1039 1438 1717 1720 2002 2023 2243
Size: 164 679 399 279 3 282 21 220
Cuts at: 2243 2426 2782 2855 2930 4184 4616 5363 5631
Size: 183 356 73 75 1254 432 747 268
Cuts at: 5631 196
Size: 924
Fragments arranged by size:
1254 924 747 679 432 399 356 282
279 268 220 183 164 75 73 21
3
EcoNI CCTnn'n_nnAGG
Cuts at: 787 2269 3063 3208 787
Size: 1482 794 145 3938
Fragments arranged by size:
3938 1482 794 145
EcoO109I rG'GnC_Cy
Cuts at: 367 804 1944 2204 2349 2823 3498 367
Size: 437 1140 260 145 474 675 3228
Fragments arranged by size:
3228 1140 675 474 437 260 145
EcoRI G'AATT_C
Cuts at: 3363 3363
Size: 6359
EcoRV GAT'ATC
Cuts at: 172 978 172
Size: 806 5553
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 407 1068 1429 4378 407
Size: 661 361 2949 2388
Fragments arranged by size:
2949 2388 661 361
FspI TGC'GCA
Cuts at: 4140 4140
Size: 6359
HaeII r_GCGC'y
Cuts at: 3793 4041 5063 6042 3793
Size: 248 1022 979 4110
Fragments arranged by size:
4110 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 284 316 613 645 1715 1747 2384 2409 2416
Size: 32 297 32 1070 32 637 25 7
Cuts at: 2416 2441 2495 2527 2985 3000 3017 3032 4201
Size: 25 54 32 458 15 17 15 1169
Cuts at: 4201 4231 4233 4263 284
Size: 30 2 30 2380
Fragments arranged by size:
2380 1169 1070 637 458 297 54 32
32 32 32 30 30 25 25 17
15 15 7 2
HincII GTy'rAC
Cuts at: 765 3398 765
Size: 2633 3726
KasI G'GCGC_C
Cuts at: 3789 4037 3789
Size: 248 6111
KpnI G_GTAC'C
Cuts at: 2945 3379 2945
Size: 434 5925
MluI A'CGCG_T
Cuts at: 5792 5792
Size: 6359
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2313 3333 5030 5214 2313
Size: 1020 1697 184 3458
Fragments arranged by size:
3458 1697 1020 184
MscI TGG'CCA
Cuts at: 479 554 4120 479
Size: 75 3566 2718
Fragments arranged by size:
3566 2718 75
MslI CAynn'nnrTG
Cuts at: 179 284 1215 2449 2663 4348 4475 179
Size: 105 931 1234 214 1685 127 2063
Fragments arranged by size:
2063 1685 1234 931 214 127 105
MspA1I CmG'CkG
Cuts at: 1721 2022 2142 2748 3470 3784 4144 5157 5402
Size: 301 120 606 722 314 360 1013 245
Cuts at: 5402 1721
Size: 2678
Fragments arranged by size:
2678 1013 722 606 360 314 301 245
120
NaeI GCC'GGC
Cuts at: 2251 4540 2251
Size: 2289 4070
NarI GG'CG_CC
Cuts at: 3790 4038 3790
Size: 248 6111
NcoI C'CATG_G
Cuts at: 107 3189 107
Size: 3082 3277
NgoMIV G'CCGG_C
Cuts at: 2249 4538 2249
Size: 2289 4070
NspI r_CATG'y
Cuts at: 1323 3412 3912 4819 1323
Size: 2089 500 907 2863
Fragments arranged by size:
2863 2089 907 500
PasI CC'CwG_GG
Cuts at: 951 964 951
Size: 13 6346
PciI A'CATG_T
Cuts at: 4815 4815
Size: 6359
PflMI CCAn_nnn'nTGG
Cuts at: 3195 3195
Size: 6359
PfoI T'CCnGG_A
Cuts at: 2832 3023 2832
Size: 191 6168
PmeI GTTT'AAAC
Cuts at: 3358 3358
Size: 6359
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5498 5530 5498
Size: 32 6327
PpuMI rG'GwC_Cy
Cuts at: 1944 2204 2349 1944
Size: 260 145 5954
Fragments arranged by size:
5954 260 145
PstI C_TGCA'G
Cuts at: 612 2472 3167 3406 4091 612
Size: 1860 695 239 685 2880
Fragments arranged by size:
2880 1860 695 685 239
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6359
PvuII CAG'CTG
Cuts at: 3784 4144 3784
Size: 360 5999
RsrII CG'GwC_CG
Cuts at: 4554 4554
Size: 6359
SacI G_AGCT'C
Cuts at: 753 3373 753
Size: 2620 3739
SacII CC_GC'GG
Cuts at: 2749 2749
Size: 6359
SalI G'TCGA_C
Cuts at: 3396 3396
Size: 6359
SanDI GG'GwC_CC
Cuts at: 2204 2204
Size: 6359
SapI GCTCTTCn'nnn_
Cuts at: 4382 4592 4382
Size: 210 6149
SbfI CC_TGCA'GG
Cuts at: 3406 3406
Size: 6359
ScaI AGT'ACT
Cuts at: 5876 5876
Size: 6359
SexAI A'CCwGG_T
Cuts at: 467 467
Size: 6359
SfcI C'TryA_G
Cuts at: 33 311 608 2468 3163 3402 4087 5080 5271
Size: 278 297 1860 695 239 685 993 191
Cuts at: 5271 33
Size: 1121
Fragments arranged by size:
1860 1121 993 695 685 297 278 239
191
SfoI GGC'GCC
Cuts at: 3791 4039 3791
Size: 248 6111
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6359
SmaI CCC'GGG
Cuts at: 2101 3381 2101
Size: 1280 5079
SmlI C'TyrA_G
Cuts at: 224 234 566 2116 2162 3514 4429 4921 5183
Size: 10 332 1550 46 1352 915 492 262
Cuts at: 5183 224
Size: 1400
Fragments arranged by size:
1550 1400 1352 915 492 332 262 46
10
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6359
SphI G_CATG'C
Cuts at: 3412 3912 3412
Size: 500 5859
SspI AAT'ATT
Cuts at: 1993 1993
Size: 6359
StuI AGG'CCT
Cuts at: 344 1705 2264 2932 3069 344
Size: 1361 559 668 137 3634
Fragments arranged by size:
3634 1361 668 559 137
StyI C'CwwG_G
Cuts at: 107 783 1571 1811 2309 3104 3189 3493 3847
Size: 676 788 240 498 795 85 304 354
Cuts at: 3847 107
Size: 2619
Fragments arranged by size:
2619 795 788 676 498 354 304 240
85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1308 3151 3253 3942 4075 4534 4743 1308
Size: 1843 102 689 133 459 209 2924
Fragments arranged by size:
2924 1843 689 459 209 133 102
TatI w'GTAC_w
Cuts at: 1388 1685 2093 3329 5874 1388
Size: 297 408 1236 2545 1873
Fragments arranged by size:
2545 1873 1236 408 297
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 921 953 2628 2660 5876 5908 921
Size: 32 1675 32 3216 32 1372
Fragments arranged by size:
3216 1675 1372 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 469 4156 469
Size: 3687 2672
XbaI T'CTAG_A
Cuts at: 322 3390 322
Size: 3068 3291
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3854 3854
Size: 6359
XmaI C'CCGG_G
Cuts at: 2099 3379 2099
Size: 1280 5079
XmnI GAAnn'nnTTC
Cuts at: 209 3047 209
Size: 2838 3521
ZraI GAC'GTC
Cuts at: 276 276
Size: 6359
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AhdI AlfI AloI ApaI AscI BaeI BcgI
BfrBI BmtI BsiWI BsmI BsmBI BssHII BstEII BstZ17I
ClaI DraIII FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PmlI PshAI PsiI
PspOMI PspXI PsrI SfiI SgrAI SnaBI SrfI SwaI
XhoI