Restriction map for
pF1KA0370
(Circular) (Six-base) MAPSORT of: pF1KA0370.seq Check: 7366 from: 1 to: 6488
pF1KA0370 6488 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2787 2787
Size: 6488
AatII G_ACGT'C
Cuts at: 465 465
Size: 6488
AccI GT'mk_AC
Cuts at: 3275 3526 3275
Size: 251 6237
Acc65I G'GTAC_C
Cuts at: 3504 3504
Size: 6488
AclI AA'CG_TT
Cuts at: 6328 6328
Size: 6488
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 137 551 861 1639 1688 2422 2806 4313 4745
Size: 414 310 778 49 734 384 1507 432
Cuts at: 4745 5492 5760 137
Size: 747 268 865
Fragments arranged by size:
1507 865 778 747 734 432 414 384
310 268 49
AflIII A'CryG_T
Cuts at: 2708 3840 4944 5921 2708
Size: 1132 1104 977 3275
Fragments arranged by size:
3275 1132 1104 977
AgeI A'CCGG_T
Cuts at: 4876 4876
Size: 6488
AleI CACnn'nnGTG
Cuts at: 1474 1864 2167 1474
Size: 390 303 5795
Fragments arranged by size:
5795 390 303
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2037 2037
Size: 6488
AlwNI CAG_nnn'CTG
Cuts at: 1980 2547 3141 3207 3446 3793 5360 1980
Size: 567 594 66 239 347 1567 3108
Fragments arranged by size:
3108 1567 594 567 347 239 66
ApaLI G'TGCA_C
Cuts at: 3409 5258 3409
Size: 1849 4639
ApoI r'AATT_y
Cuts at: 157 1090 3366 3492 5841 6097 157
Size: 933 2276 126 2349 256 548
Fragments arranged by size:
2349 2276 933 548 256 126
AseI AT'TA_AT
Cuts at: 20 20
Size: 6488
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6488
AvaI C'yCGr_G
Cuts at: 1008 2384 2505 3508 1008
Size: 1376 121 1003 3988
Fragments arranged by size:
3988 1376 1003 121
BamHI G'GATC_C
Cuts at: 3513 3513
Size: 6488
BanI G'GyrC_C
Cuts at: 3504 3918 4166 4201 3504
Size: 414 248 35 5791
Fragments arranged by size:
5791 414 248 35
BanII G_rGCy'C
Cuts at: 1196 1616 1783 2283 2930 3032 3502 4532 1196
Size: 420 167 500 647 102 470 1030 3152
Fragments arranged by size:
3152 1030 647 500 470 420 167 102
BbeI G_GCGC'C
Cuts at: 3922 4170 3922
Size: 248 6240
BbsI GAAGACnn'nnnn_
Cuts at: 565 565
Size: 6488
BbvCI CC'TCA_GC
Cuts at: 3008 3008
Size: 6488
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2516 2550 2516
Size: 34 6454
BciVI GTATCCnnnnn_n'
Cuts at: 2885 4386 5147 6488 2885
Size: 1501 761 1341 2885
Fragments arranged by size:
2885 1501 1341 761
BclI T'GATC_A
Cuts at: 320 548 2186 4008 320
Size: 228 1638 1822 2800
Fragments arranged by size:
2800 1822 1638 228
BglII A'GATC_T
Cuts at: 3155 4003 6040 3155
Size: 848 2037 3603
Fragments arranged by size:
3603 2037 848
BlpI GC'TnA_GC
Cuts at: 2623 3600 2623
Size: 977 5511
Bme1580I G_kGCm'C
Cuts at: 3413 4113 4206 5262 3413
Size: 700 93 1056 4639
Fragments arranged by size:
4639 1056 700 93
BmrI ACTGGGnnnn_n'
Cuts at: 776 1272 2402 3027 3700 3781 4116 6260 776
Size: 496 1130 625 673 81 335 2144 1004
Fragments arranged by size:
2144 1130 1004 673 625 496 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1290 2520 2913 4855 1290
Size: 1230 393 1942 2923
Fragments arranged by size:
2923 1942 1230 393
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 249 261 1537 2976 3058 3184 249
Size: 12 1276 1439 82 126 3553
Fragments arranged by size:
3553 1439 1276 126 82 12
Bpu10I CC'TnA_GC
Cuts at: 3008 3008
Size: 6488
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1797 3664 4543 5035 5333 1797
Size: 1867 879 492 298 2952
Fragments arranged by size:
2952 1867 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2157 2571 6192 2157
Size: 414 3621 2453
Fragments arranged by size:
3621 2453 414
BsaAI yAC'GTr
Cuts at: 3841 3841
Size: 6488
BsaBI GATnn'nnATC
Cuts at: 6 364 1547 2191 3247 4002 6039 6
Size: 358 1183 644 1056 755 2037 455
Fragments arranged by size:
2037 1183 1056 755 644 455 358
BsaHI Gr'CG_yC
Cuts at: 462 976 3919 4167 4869 6365 462
Size: 514 2943 248 702 1496 585
Fragments arranged by size:
2943 1496 702 585 514 248
BsaWI w'CCGG_w
Cuts at: 425 2936 3161 3677 4198 4876 5150 5297 5708
Size: 2511 225 516 521 678 274 147 411
Cuts at: 5708 425
Size: 1205
Fragments arranged by size:
2511 1205 678 521 516 411 274 225
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 247 277 820 850 1618 1648 1765 1795 2857
Size: 30 543 30 768 30 117 30 1062
Cuts at: 2857 2887 247
Size: 30 3848
Fragments arranged by size:
3848 1062 768 543 117 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 217 631 1111 1534 3025 3253 217
Size: 414 480 423 1491 228 3452
Fragments arranged by size:
3452 1491 480 423 414 228
BseYI C'CCAG_C
Cuts at: 224 1029 1511 2842 3357 3913 4671 5248 5744
Size: 805 482 1331 515 556 758 577 496
Cuts at: 5744 224
Size: 968
Fragments arranged by size:
1331 968 805 758 577 556 515 496
482
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 667 1741 1823 2224 2360 667
Size: 1074 82 401 136 4795
Fragments arranged by size:
4795 1074 401 136 82
BsiEI CG_ry'CG
Cuts at: 104 1026 4030 4076 5284 104
Size: 922 3004 46 1208 1308
Fragments arranged by size:
3004 1308 1208 922 46
BsiHKAI G_wGCw'C
Cuts at: 1196 1463 1616 1783 2930 3413 3502 4280 4470
Size: 267 153 167 1147 483 89 778 190
Cuts at: 4470 5262 1196
Size: 792 2422
Fragments arranged by size:
2422 1147 792 778 483 267 190 167
153 89
Bsp1286I G_dGCh'C
Cuts at: 1196 1463 1616 1783 2283 2930 3032 3413 3502
Size: 267 153 167 500 647 102 381 89
Cuts at: 3502 4113 4206 4280 4470 4532 5262 1196
Size: 611 93 74 190 62 730 2422
Fragments arranged by size:
2422 730 647 611 500 381 267 190
167 153 102 93 89 74 62
BspEI T'CCGG_A
Cuts at: 425 2936 3161 5708 425
Size: 2511 225 2547 1205
Fragments arranged by size:
2547 2511 1205 225
BspHI T'CATG_A
Cuts at: 1338 5664 1338
Size: 4326 2162
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2787 3395 3437 3538 3752 4054 4435 2787
Size: 608 42 101 214 302 381 4840
Fragments arranged by size:
4840 608 381 302 214 101 42
BsrBI CCG'CTC
Cuts at: 50 4780 4834 6318 6483 50
Size: 4730 54 1484 165 55
Fragments arranged by size:
4730 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2882 4400 2882
Size: 1518 4970
BsrFI r'CCGG_y
Cuts at: 1025 1748 2036 4486 4667 4876 1025
Size: 723 288 2450 181 209 2637
Fragments arranged by size:
2637 2450 723 288 209 181
BsrGI T'GTAC_A
Cuts at: 172 293 1913 172
Size: 121 1620 4747
Fragments arranged by size:
4747 1620 121
BssSI C'ACGA_G
Cuts at: 2746 4759 5117 2746
Size: 2013 358 4117
Fragments arranged by size:
4117 2013 358
BstAPI GCAn_nnn'nTGC
Cuts at: 654 2762 3865 654
Size: 2108 1103 3277
Fragments arranged by size:
3277 2108 1103
BstBI TT'CG_AA
Cuts at: 4849 4849
Size: 6488
BstXI CCAn_nnnn'nTGG
Cuts at: 972 1608 2982 972
Size: 636 1374 4478
Fragments arranged by size:
4478 1374 636
BstYI r'GATC_y
Cuts at: 1 1897 2789 3155 3513 3980 4003 4338 4584
Size: 1896 892 366 358 467 23 335 246
Cuts at: 4584 5596 5682 5694 6040 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1896 1012 892 467 449 366 358 346
335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 738 738
Size: 6488
BtgI C'CryG_G
Cuts at: 107 1796 2078 2305 107
Size: 1689 282 227 4290
Fragments arranged by size:
4290 1689 282 227
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2835 3234 4438 4619 4782 2835
Size: 399 1204 181 163 4541
Fragments arranged by size:
4541 1204 399 181 163
BtsI GCAGTG_nn'
Cuts at: 1758 2031 2207 2341 3731 1758
Size: 273 176 134 1390 4515
Fragments arranged by size:
4515 1390 273 176 134
DraI TTT'AAA
Cuts at: 3096 3487 3096
Size: 391 6097
DraIII CAC_nnn'GTG
Cuts at: 1173 1173
Size: 6488
DrdI GACnn_nn'nnGTC
Cuts at: 4194 5052 4194
Size: 858 5630
EaeI y'GGCC_r
Cuts at: 1023 1040 1598 1808 2159 2308 2984 4073 4247
Size: 17 558 210 351 149 676 1089 174
Cuts at: 4247 4638 4665 6010 1023
Size: 391 27 1345 1501
Fragments arranged by size:
1501 1345 1089 676 558 391 351 210
174 149 27 17
EagI C'GGCC_G
Cuts at: 1023 4073 1023
Size: 3050 3438
EarI CTCTTCn'nnn_
Cuts at: 566 880 1171 1184 3112 4511 4721 566
Size: 314 291 13 1928 1399 210 2333
Fragments arranged by size:
2333 1928 1399 314 291 210 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1750 3378 4696 5006 5152 6298 1750
Size: 1628 1318 310 146 1146 1940
Fragments arranged by size:
1940 1628 1318 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1194 1614 1781 2928 3500 1194
Size: 420 167 1147 572 4182
Fragments arranged by size:
4182 1147 572 420 167
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 137 249 261 551 861 1537 1639 1688 2422
Size: 112 12 290 310 676 102 49 734
Cuts at: 2422 2806 2976 3058 3184 4313 4745 5492 5760
Size: 384 170 82 126 1129 432 747 268
Cuts at: 5760 137
Size: 865
Fragments arranged by size:
1129 865 747 734 676 432 384 310
290 268 170 126 112 102 82 49
12
EcoNI CCTnn'n_nnAGG
Cuts at: 129 1789 2746 129
Size: 1660 957 3871
Fragments arranged by size:
3871 1660 957
EcoO109I rG'GnC_Cy
Cuts at: 342 594 1035 3204 3384 3627 342
Size: 252 441 2169 180 243 3203
Fragments arranged by size:
3203 2169 441 252 243 180
EcoRI G'AATT_C
Cuts at: 3492 3492
Size: 6488
EcoRV GAT'ATC
Cuts at: 1365 1365
Size: 6488
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3174 4507 3174
Size: 1333 5155
FspI TGC'GCA
Cuts at: 4269 4269
Size: 6488
HaeII r_GCGC'y
Cuts at: 3922 4170 5192 6171 3922
Size: 248 1022 979 4239
Fragments arranged by size:
4239 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1765 1797 2931 2963 3376 3408 4330 4360 4362
Size: 32 1134 32 413 32 922 30 2
Cuts at: 4362 4392 1765
Size: 30 3861
Fragments arranged by size:
3861 1134 922 413 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 984 3276 3423 3527 984
Size: 2292 147 104 3945
Fragments arranged by size:
3945 2292 147 104
HpaI GTT'AAC
Cuts at: 984 984
Size: 6488
KasI G'GCGC_C
Cuts at: 3918 4166 3918
Size: 248 6240
KpnI G_GTAC'C
Cuts at: 3508 3508
Size: 6488
MluI A'CGCG_T
Cuts at: 5921 5921
Size: 6488
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 862 1505 5159 5343 862
Size: 643 3654 184 2007
Fragments arranged by size:
3654 2007 643 184
MscI TGG'CCA
Cuts at: 1042 1600 2161 2310 2986 4249 1042
Size: 558 561 149 676 1263 3281
Fragments arranged by size:
3281 1263 676 561 558 149
MslI CAynn'nnrTG
Cuts at: 1474 1864 1963 2167 4477 4604 1474
Size: 390 99 204 2310 127 3358
Fragments arranged by size:
3358 2310 390 204 127 99
MspA1I CmG'CkG
Cuts at: 1515 1569 3323 3599 3913 4273 5286 5531 1515
Size: 54 1754 276 314 360 1013 245 2472
Fragments arranged by size:
2472 1754 1013 360 314 276 245 54
NaeI GCC'GGC
Cuts at: 1027 1750 4669 1027
Size: 723 2919 2846
Fragments arranged by size:
2919 2846 723
NarI GG'CG_CC
Cuts at: 3919 4167 3919
Size: 248 6240
NcoI C'CATG_G
Cuts at: 107 2078 107
Size: 1971 4517
NgoMIV G'CCGG_C
Cuts at: 1025 1748 4667 1025
Size: 723 2919 2846
Fragments arranged by size:
2919 2846 723
NspI r_CATG'y
Cuts at: 1972 2712 3541 4041 4948 1972
Size: 740 829 500 907 3512
Fragments arranged by size:
3512 907 829 740 500
PciI A'CATG_T
Cuts at: 2708 4944 2708
Size: 2236 4252
PflMI CCAn_nnn'nTGG
Cuts at: 2148 2268 3207 2148
Size: 120 939 5429
Fragments arranged by size:
5429 939 120
PfoI T'CCnGG_A
Cuts at: 2807 2807
Size: 6488
PmeI GTTT'AAAC
Cuts at: 3487 3487
Size: 6488
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 768 800 991 1023 3019 3051 5627 5659 768
Size: 32 191 32 1996 32 2576 32 1597
Fragments arranged by size:
2576 1996 1597 191 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 342 594 1035 3204 3384 342
Size: 252 441 2169 180 3446
Fragments arranged by size:
3446 2169 441 252 180
PstI C_TGCA'G
Cuts at: 1142 1793 1994 2202 2552 2798 3236 3535 4220
Size: 651 201 208 350 246 438 299 685
Cuts at: 4220 1142
Size: 3410
Fragments arranged by size:
3410 685 651 438 350 299 246 208
201
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6488
PvuII CAG'CTG
Cuts at: 1515 3323 3913 4273 1515
Size: 1808 590 360 3730
Fragments arranged by size:
3730 1808 590 360
RsrII CG'GwC_CG
Cuts at: 4683 4683
Size: 6488
SacI G_AGCT'C
Cuts at: 1196 1616 1783 2930 3502 1196
Size: 420 167 1147 572 4182
Fragments arranged by size:
4182 1147 572 420 167
SalI G'TCGA_C
Cuts at: 3274 3525 3274
Size: 251 6237
SanDI GG'GwC_CC
Cuts at: 342 342
Size: 6488
SapI GCTCTTCn'nnn_
Cuts at: 566 1171 1184 4511 4721 566
Size: 605 13 3327 210 2333
Fragments arranged by size:
3327 2333 605 210 13
SbfI CC_TGCA'GG
Cuts at: 2798 3535 2798
Size: 737 5751
ScaI AGT'ACT
Cuts at: 1816 6005 1816
Size: 4189 2299
SexAI A'CCwGG_T
Cuts at: 641 641
Size: 6488
SfcI C'TryA_G
Cuts at: 33 1138 1789 1990 2198 2548 2794 3232 3438
Size: 1105 651 201 208 350 246 438 206
Cuts at: 3438 3531 4216 5209 5400 33
Size: 93 685 993 191 1121
Fragments arranged by size:
1121 1105 993 685 651 438 350 246
208 206 201 191 93
SfoI GGC'GCC
Cuts at: 3920 4168 3920
Size: 248 6240
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6488
SmaI CCC'GGG
Cuts at: 2386 3510 2386
Size: 1124 5364
SmlI C'TyrA_G
Cuts at: 1776 3643 4558 5050 5312 1776
Size: 1867 915 492 262 2952
Fragments arranged by size:
2952 1867 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6488
SphI G_CATG'C
Cuts at: 1972 3541 4041 1972
Size: 1569 500 4419
Fragments arranged by size:
4419 1569 500
StuI AGG'CCT
Cuts at: 2651 2651
Size: 6488
StyI C'CwwG_G
Cuts at: 107 540 1151 2078 2108 3622 3976 107
Size: 433 611 927 30 1514 354 2619
Fragments arranged by size:
2619 1514 927 611 433 354 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2656 3415 4071 4204 4663 4872 2656
Size: 759 656 133 459 209 4272
Fragments arranged by size:
4272 759 656 459 209 133
TatI w'GTAC_w
Cuts at: 172 254 293 1316 1814 1913 2658 6003 172
Size: 82 39 1023 498 99 745 3345 657
Fragments arranged by size:
3345 1023 745 657 498 99 82 39
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1618 1650 6005 6037 1618
Size: 32 4355 32 2069
Fragments arranged by size:
4355 2069 32 32
Tth111I GACn'n_nGTC
Cuts at: 4285 4285
Size: 6488
XbaI T'CTAG_A
Cuts at: 3519 3519
Size: 6488
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 390 822 3261 3983 390
Size: 432 2439 722 2895
Fragments arranged by size:
2895 2439 722 432
XmaI C'CCGG_G
Cuts at: 2384 3508 2384
Size: 1124 5364
XmnI GAAnn'nnTTC
Cuts at: 161 1430 2212 2558 3157 161
Size: 1269 782 346 599 3492
Fragments arranged by size:
3492 1269 782 599 346
ZraI GAC'GTC
Cuts at: 463 463
Size: 6488
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AloI ApaI AscI AvrII BaeI
BfrBI BglI BmgBI BmtI BsiWI BsmI BsmBI BssHII
BstEII BstZ17I ClaI CspCI FseI FspAI HindIII MfeI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI