Restriction map for
pF1KA0371
(Circular) (Six-base) MAPSORT of: pF1KA0371.seq Check: 8378 from: 1 to: 6707
pF1KA0371 6707 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 477 3411 477
Size: 2934 3773
AccI GT'mk_AC
Cuts at: 3745 3745
Size: 6707
Acc65I G'GTAC_C
Cuts at: 2569 3723 2569
Size: 1154 5553
AclI AA'CG_TT
Cuts at: 1521 3701 6547 1521
Size: 2180 2846 1681
Fragments arranged by size:
2846 2180 1681
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 460 989 1549 2744 3283 3425 4532 4964
Size: 279 529 560 1195 539 142 1107 432
Cuts at: 4964 5711 5979 181
Size: 747 268 909
Fragments arranged by size:
1195 1107 909 747 560 539 529 432
279 268 142
AfeI AGC'GCT
Cuts at: 1292 1292
Size: 6707
AflIII A'CryG_T
Cuts at: 1705 2648 2839 4059 5163 6140 1705
Size: 943 191 1220 1104 977 2272
Fragments arranged by size:
2272 1220 1104 977 943 191
AgeI A'CCGG_T
Cuts at: 1481 5095 1481
Size: 3614 3093
AleI CACnn'nnGTG
Cuts at: 1861 2119 1861
Size: 258 6449
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1228 1228
Size: 6707
AlwNI CAG_nnn'CTG
Cuts at: 749 1075 1191 2908 3198 3261 3288 4012 5579
Size: 326 116 1717 290 63 27 724 1567
Cuts at: 5579 749
Size: 1877
Fragments arranged by size:
1877 1717 1567 724 326 290 116 63
27
ApaI G_GGCC'C
Cuts at: 2687 2687
Size: 6707
ApaLI G'TGCA_C
Cuts at: 1203 1877 5477 1203
Size: 674 3600 2433
Fragments arranged by size:
3600 2433 674
ApoI r'AATT_y
Cuts at: 1471 3711 6060 6316 1471
Size: 2240 2349 256 1862
Fragments arranged by size:
2349 2240 1862 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6707
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6707
AvaI C'yCGr_G
Cuts at: 941 3727 941
Size: 2786 3921
AvrII C'CTAG_G
Cuts at: 2103 2103
Size: 6707
BamHI G'GATC_C
Cuts at: 2082 3732 2082
Size: 1650 5057
BanI G'GyrC_C
Cuts at: 779 1897 2131 2569 2896 3723 4137 4385 4420
Size: 1118 234 438 327 827 414 248 35
Cuts at: 4420 779
Size: 3066
Fragments arranged by size:
3066 1118 827 438 414 327 248 234
35
BanII G_rGCy'C
Cuts at: 692 2423 2687 3721 4751 692
Size: 1731 264 1034 1030 2648
Fragments arranged by size:
2648 1731 1034 1030 264
BbeI G_GCGC'C
Cuts at: 4141 4389 4141
Size: 248 6459
BbsI GAAGACnn'nnnn_
Cuts at: 2302 2302
Size: 6707
BbvCI CC'TCA_GC
Cuts at: 2162 2310 2162
Size: 148 6559
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1858 1892 1858
Size: 34 6673
BciVI GTATCCnnnnn_n'
Cuts at: 4605 5366 6707 4605
Size: 761 1341 4605
Fragments arranged by size:
4605 1341 761
BclI T'GATC_A
Cuts at: 4227 4227
Size: 6707
BglI GCCn_nnn'nGGC
Cuts at: 2069 2233 2069
Size: 164 6543
BglII A'GATC_T
Cuts at: 155 483 2701 4222 6259 155
Size: 328 2218 1521 2037 603
Fragments arranged by size:
2218 2037 1521 603 328
BlpI GC'TnA_GC
Cuts at: 1388 2056 3819 1388
Size: 668 1763 4276
Fragments arranged by size:
4276 1763 668
Bme1580I G_kGCm'C
Cuts at: 1207 1881 2687 2901 4332 4425 5481 1207
Size: 674 806 214 1431 93 1056 2433
Fragments arranged by size:
2433 1431 1056 806 674 214 93
BmgBI CAC'GTC
Cuts at: 975 975
Size: 6707
BmrI ACTGGGnnnn_n'
Cuts at: 1522 2350 2539 3602 3919 4000 4335 6479 1522
Size: 828 189 1063 317 81 335 2144 1750
Fragments arranged by size:
2144 1750 1063 828 335 317 189 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2399 3417 5074 2399
Size: 1018 1657 4032
Fragments arranged by size:
4032 1657 1018
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 654 2096 2428 2494 2935 3295 3540 3639 654
Size: 1442 332 66 441 360 245 99 3722
Fragments arranged by size:
3722 1442 441 360 332 245 99 66
Bpu10I CC'TnA_GC
Cuts at: 1921 2162 2310 1921
Size: 241 148 6318
Fragments arranged by size:
6318 241 148
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 668 3048 3883 4762 5254 5552 154
Size: 514 2380 835 879 492 298 1309
Fragments arranged by size:
2380 1309 879 835 514 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 545 956 2608 6411 545
Size: 411 1652 3803 841
Fragments arranged by size:
3803 1652 841 411
BsaAI yAC'GTr
Cuts at: 4060 4060
Size: 6707
BsaBI GATnn'nnATC
Cuts at: 6 191 860 1319 4221 6258 6
Size: 185 669 459 2902 2037 455
Fragments arranged by size:
2902 2037 669 459 455 185
BsaHI Gr'CG_yC
Cuts at: 4138 4386 5088 6584 4138
Size: 248 702 1496 4261
Fragments arranged by size:
4261 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1481 3896 4417 5095 5369 5516 5927 1481
Size: 2415 521 678 274 147 411 2261
Fragments arranged by size:
2415 2261 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3268 3298 3268
Size: 30 6677
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1112 1191 2218 2373 2422 1112
Size: 79 1027 155 49 5397
Fragments arranged by size:
5397 1027 155 79 49
BseYI C'CCAG_C
Cuts at: 1355 1887 2011 2752 3413 3586 4132 4890 5467
Size: 532 124 741 661 173 546 758 577
Cuts at: 5467 5963 1355
Size: 496 2099
Fragments arranged by size:
2099 758 741 661 577 546 532 496
173 124
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 579 1642 1847 579
Size: 1063 205 5439
Fragments arranged by size:
5439 1063 205
BsiEI CG_ry'CG
Cuts at: 104 907 1330 4249 4295 5503 104
Size: 803 423 2919 46 1208 1308
Fragments arranged by size:
2919 1308 1208 803 423 46
BsiHKAI G_wGCw'C
Cuts at: 232 692 1207 1881 2039 3721 4499 4689 5481
Size: 460 515 674 158 1682 778 190 792
Cuts at: 5481 232
Size: 1458
Fragments arranged by size:
1682 1458 792 778 674 515 460 190
158
BsmI GAATG_Cn'
Cuts at: 352 1114 1603 352
Size: 762 489 5456
Fragments arranged by size:
5456 762 489
Bsp1286I G_dGCh'C
Cuts at: 232 692 1207 1881 2039 2423 2687 2901 3721
Size: 460 515 674 158 384 264 214 820
Cuts at: 3721 4332 4425 4499 4689 4751 5481 232
Size: 611 93 74 190 62 730 1458
Fragments arranged by size:
1458 820 730 674 611 515 460 384
264 214 190 158 93 74 62
BspEI T'CCGG_A
Cuts at: 5927 5927
Size: 6707
BspHI T'CATG_A
Cuts at: 5883 5883
Size: 6707
BspMI ACCTGCnnnn'nnnn_
Cuts at: 477 1844 2232 3411 3757 3971 4273 4654 477
Size: 1367 388 1179 346 214 302 381 2530
Fragments arranged by size:
2530 1367 1179 388 381 346 302 214
BsrBI CCG'CTC
Cuts at: 50 4999 5053 6537 6702 50
Size: 4949 54 1484 165 55
Fragments arranged by size:
4949 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 263 795 800 2898 2976 3683 4619 263
Size: 532 5 2098 78 707 936 2351
Fragments arranged by size:
2351 2098 936 707 532 78 5
BsrFI r'CCGG_y
Cuts at: 1329 1481 1925 1994 2039 4705 4886 5095 1329
Size: 152 444 69 45 2666 181 209 2941
Fragments arranged by size:
2941 2666 444 209 181 152 69 45
BsrGI T'GTAC_A
Cuts at: 3173 3173
Size: 6707
BssSI C'ACGA_G
Cuts at: 1438 4978 5336 1438
Size: 3540 358 2809
Fragments arranged by size:
3540 2809 358
BstAPI GCAn_nnn'nTGC
Cuts at: 386 505 3626 4084 386
Size: 119 3121 458 3009
Fragments arranged by size:
3121 3009 458 119
BstBI TT'CG_AA
Cuts at: 2496 5068 2496
Size: 2572 4135
BstXI CCAn_nnnn'nTGG
Cuts at: 790 1863 790
Size: 1073 5634
BstYI r'GATC_y
Cuts at: 1 155 483 1218 2082 2701 3732 4199 4222
Size: 154 328 735 864 619 1031 467 23
Cuts at: 4222 4557 4803 5815 5901 5913 6259 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1031 1012 864 735 619 467 449 346
335 328 246 154 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2303 2303
Size: 6707
BtgI C'CryG_G
Cuts at: 107 2744 107
Size: 2637 4070
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4657 4838 5001 4657
Size: 181 163 6363
Fragments arranged by size:
6363 181 163
BtsI GCAGTG_nn'
Cuts at: 430 1341 1546 1835 3477 3525 3950 430
Size: 911 205 289 1642 48 425 3187
Fragments arranged by size:
3187 1642 911 425 289 205 48
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 651 686 651
Size: 35 6672
DraI TTT'AAA
Cuts at: 591 3706 591
Size: 3115 3592
DrdI GACnn_nn'nnGTC
Cuts at: 4413 5271 4413
Size: 858 5849
EaeI y'GGCC_r
Cuts at: 1327 1464 1988 3470 4292 4466 4857 4884 6229
Size: 137 524 1482 822 174 391 27 1345
Cuts at: 6229 1327
Size: 1805
Fragments arranged by size:
1805 1482 1345 822 524 391 174 137
27
EagI C'GGCC_G
Cuts at: 1327 4292 1327
Size: 2965 3742
EarI CTCTTCn'nnn_
Cuts at: 110 436 493 2498 3259 4730 4940 110
Size: 326 57 2005 761 1471 210 1877
Fragments arranged by size:
2005 1877 1471 761 326 210 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4915 5225 5371 6517 4915
Size: 310 146 1146 5105
Fragments arranged by size:
5105 1146 310 146
EcoICRI GAG'CTC
Cuts at: 690 3719 690
Size: 3029 3678
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 460 654 989 1549 2096 2428 2494 2744
Size: 279 194 335 560 547 332 66 250
Cuts at: 2744 2935 3283 3295 3425 3540 3639 4532 4964
Size: 191 348 12 130 115 99 893 432
Cuts at: 4964 5711 5979 181
Size: 747 268 909
Fragments arranged by size:
909 893 747 560 547 432 348 335
332 279 268 250 194 191 130 115
99 66 12
EcoO109I rG'GnC_Cy
Cuts at: 746 1434 2265 2448 2478 2684 2783 2918 3846
Size: 688 831 183 30 206 99 135 928
Cuts at: 3846 746
Size: 3607
Fragments arranged by size:
3607 928 831 688 206 183 135 99
30
EcoRI G'AATT_C
Cuts at: 3711 3711
Size: 6707
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1624 2551 2899 4726 1624
Size: 927 348 1827 3605
Fragments arranged by size:
3605 1827 927 348
FspI TGC'GCA
Cuts at: 4488 4488
Size: 6707
HaeII r_GCGC'y
Cuts at: 1294 2001 2046 3198 4141 4389 5411 6390 1294
Size: 707 45 1152 943 248 1022 979 1611
Fragments arranged by size:
1611 1152 1022 979 943 707 248 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 275 307 475 507 889 921 1045 1077 1203
Size: 32 168 32 382 32 124 32 126
Cuts at: 1203 1235 2349 2381 3151 3183 4549 4579 4581
Size: 32 1114 32 770 32 1366 30 2
Cuts at: 4581 4611 275
Size: 30 2371
Fragments arranged by size:
2371 1366 1114 770 382 168 126 124
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 926 3746 926
Size: 2820 3887
HindIII A'AGCT_T
Cuts at: 884 1039 884
Size: 155 6552
KasI G'GCGC_C
Cuts at: 4137 4385 4137
Size: 248 6459
KpnI G_GTAC'C
Cuts at: 2573 3727 2573
Size: 1154 5553
MluI A'CGCG_T
Cuts at: 6140 6140
Size: 6707
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 486 668 1053 3213 3722 5378 5562 486
Size: 182 385 2160 509 1656 184 1631
Fragments arranged by size:
2160 1656 1631 509 385 184 182
MscI TGG'CCA
Cuts at: 1466 1990 4468 1466
Size: 524 2478 3705
Fragments arranged by size:
3705 2478 524
MslI CAynn'nnrTG
Cuts at: 1210 1656 1861 2119 2580 2653 3169 3308 3442
Size: 446 205 258 461 73 516 139 134
Cuts at: 3442 3482 4696 4823 1210
Size: 40 1214 127 3094
Fragments arranged by size:
3094 1214 516 461 446 258 205 139
134 127 73 40
MspA1I CmG'CkG
Cuts at: 174 809 1072 1326 1874 3027 3413 3818 4132
Size: 635 263 254 548 1153 386 405 314
Cuts at: 4132 4492 5505 5750 174
Size: 360 1013 245 1131
Fragments arranged by size:
1153 1131 1013 635 548 405 386 360
314 263 254 245
NaeI GCC'GGC
Cuts at: 1927 1996 4888 1927
Size: 69 2892 3746
Fragments arranged by size:
3746 2892 69
NarI GG'CG_CC
Cuts at: 4138 4386 4138
Size: 248 6459
NcoI C'CATG_G
Cuts at: 107 2744 107
Size: 2637 4070
NgoMIV G'CCGG_C
Cuts at: 1925 1994 4886 1925
Size: 69 2892 3746
Fragments arranged by size:
3746 2892 69
NspI r_CATG'y
Cuts at: 577 1309 1709 2652 3168 3760 4260 5167 577
Size: 732 400 943 516 592 500 907 2117
Fragments arranged by size:
2117 943 907 732 592 516 500 400
PasI CC'CwG_GG
Cuts at: 2456 2456
Size: 6707
PciI A'CATG_T
Cuts at: 1705 2648 5163 1705
Size: 943 2515 3249
Fragments arranged by size:
3249 2515 943
PflMI CCAn_nnn'nTGG
Cuts at: 1377 1377
Size: 6707
PfoI T'CCnGG_A
Cuts at: 179 748 1221 3254 179
Size: 569 473 2033 3632
Fragments arranged by size:
3632 2033 569 473
PmeI GTTT'AAAC
Cuts at: 3706 3706
Size: 6707
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5846 5878 5846
Size: 32 6675
PpuMI rG'GwC_Cy
Cuts at: 746 1434 2265 2448 2478 746
Size: 688 831 183 30 4975
Fragments arranged by size:
4975 831 688 183 30
PspOMI G'GGCC_C
Cuts at: 2683 2683
Size: 6707
PstI C_TGCA'G
Cuts at: 1541 2005 3046 3388 3532 3754 4439 1541
Size: 464 1041 342 144 222 685 3809
Fragments arranged by size:
3809 1041 685 464 342 222 144
PvuI CG_AT'CG
Cuts at: 104 907 104
Size: 803 5904
PvuII CAG'CTG
Cuts at: 174 1072 1874 3027 3413 4132 4492 174
Size: 898 802 1153 386 719 360 2389
Fragments arranged by size:
2389 1153 898 802 719 386 360
RsrII CG'GwC_CG
Cuts at: 4902 4902
Size: 6707
SacI G_AGCT'C
Cuts at: 692 3721 692
Size: 3029 3678
SalI G'TCGA_C
Cuts at: 3744 3744
Size: 6707
SapI GCTCTTCn'nnn_
Cuts at: 4730 4940 4730
Size: 210 6497
SbfI CC_TGCA'GG
Cuts at: 3754 3754
Size: 6707
ScaI AGT'ACT
Cuts at: 6224 6224
Size: 6707
SfcI C'TryA_G
Cuts at: 33 774 1537 2001 2642 3042 3384 3528 3630
Size: 741 763 464 641 400 342 144 102
Cuts at: 3630 3750 4435 5428 5619 33
Size: 120 685 993 191 1121
Fragments arranged by size:
1121 993 763 741 685 641 464 400
342 191 144 120 102
SfoI GGC'GCC
Cuts at: 4139 4387 4139
Size: 248 6459
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6707
SgrAI Cr'CCGG_yG
Cuts at: 2039 2039
Size: 6707
SmaI CCC'GGG
Cuts at: 3729 3729
Size: 6707
SmlI C'TyrA_G
Cuts at: 133 683 941 3063 3862 4777 5269 5531 133
Size: 550 258 2122 799 915 492 262 1309
Fragments arranged by size:
2122 1309 915 799 550 492 262 258
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6707
SphI G_CATG'C
Cuts at: 1309 3760 4260 1309
Size: 2451 500 3756
Fragments arranged by size:
3756 2451 500
StuI AGG'CCT
Cuts at: 2308 2308
Size: 6707
StyI C'CwwG_G
Cuts at: 107 1670 2103 2688 2744 2817 3841 4195 107
Size: 1563 433 585 56 73 1024 354 2619
Fragments arranged by size:
2619 1563 1024 585 433 354 73 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2332 2982 4290 4423 4882 5091 2332
Size: 650 1308 133 459 209 3948
Fragments arranged by size:
3948 1308 650 459 209 133
TatI w'GTAC_w
Cuts at: 659 1338 3173 6222 659
Size: 679 1835 3049 1144
Fragments arranged by size:
3049 1835 1144 679
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1435 1467 2615 2647 6224 6256 1435
Size: 32 1148 32 3577 32 1886
Fragments arranged by size:
3577 1886 1148 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4504 4504
Size: 6707
XbaI T'CTAG_A
Cuts at: 3738 3738
Size: 6707
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 827 4202 827
Size: 3375 3332
XhoI C'TCGA_G
Cuts at: 941 941
Size: 6707
XmaI C'CCGG_G
Cuts at: 3727 3727
Size: 6707
XmnI GAAnn'nnTTC
Cuts at: 2531 3547 2531
Size: 1016 5691
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI BaeI BfrBI BmtI
BsiWI BsmBI BssHII BstEII BstZ17I ClaI DraIII EcoNI
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PmlI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SnaBI SrfI SspI SwaI
ZraI