Restriction map for
pF1KA0389
(Circular) (Six-base) MAPSORT of: pF1KA0389.seq Check: 6420 from: 1 to: 6968
pF1KA0389 6968 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 4006 4006
Size: 6968
Acc65I G'GTAC_C
Cuts at: 1332 3984 1332
Size: 2652 4316
AclI AA'CG_TT
Cuts at: 6808 6808
Size: 6968
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 416 896 1166 1219 1594 2158 2570 3077 3877
Size: 480 270 53 375 564 412 507 800
Cuts at: 3877 4793 5225 5972 6240 416
Size: 916 432 747 268 1144
Fragments arranged by size:
1144 916 800 747 564 507 480 432
412 375 270 268 53
AflII C'TTAA_G
Cuts at: 1009 1009
Size: 6968
AflIII A'CryG_T
Cuts at: 2255 4320 5424 6401 2255
Size: 2065 1104 977 2822
Fragments arranged by size:
2822 2065 1104 977
AgeI A'CCGG_T
Cuts at: 5356 5356
Size: 6968
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2581 2581
Size: 6968
AlwNI CAG_nnn'CTG
Cuts at: 2483 3316 3954 4273 5840 2483
Size: 833 638 319 1567 3611
Fragments arranged by size:
3611 1567 833 638 319
ApaLI G'TGCA_C
Cuts at: 1068 5738 1068
Size: 4670 2298
ApoI r'AATT_y
Cuts at: 362 592 730 2061 2159 2401 2662 2732 3271
Size: 230 138 1331 98 242 261 70 539
Cuts at: 3271 3418 3972 6321 6577 362
Size: 147 554 2349 256 753
Fragments arranged by size:
2349 1331 753 554 539 261 256 242
230 147 138 98 70
AseI AT'TA_AT
Cuts at: 20 20
Size: 6968
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6968
AvaI C'yCGr_G
Cuts at: 2701 3988 2701
Size: 1287 5681
BamHI G'GATC_C
Cuts at: 2295 3993 2295
Size: 1698 5270
BanI G'GyrC_C
Cuts at: 132 1309 1332 3984 4398 4646 4681 132
Size: 1177 23 2652 414 248 35 2419
Fragments arranged by size:
2652 2419 1177 414 248 35 23
BanII G_rGCy'C
Cuts at: 763 3176 3398 3982 5012 763
Size: 2413 222 584 1030 2719
Fragments arranged by size:
2719 2413 1030 584 222
BbeI G_GCGC'C
Cuts at: 136 4402 4650 136
Size: 4266 248 2454
Fragments arranged by size:
4266 2454 248
BbsI GAAGACnn'nnnn_
Cuts at: 700 2939 700
Size: 2239 4729
BbvCI CC'TCA_GC
Cuts at: 2884 3548 2884
Size: 664 6304
BciVI GTATCCnnnnn_n'
Cuts at: 4866 5627 6968 4866
Size: 761 1341 4866
Fragments arranged by size:
4866 1341 761
BclI T'GATC_A
Cuts at: 1404 1725 2406 4488 1404
Size: 321 681 2082 3884
Fragments arranged by size:
3884 2082 681 321
BglI GCCn_nnn'nGGC
Cuts at: 3202 3202
Size: 6968
BglII A'GATC_T
Cuts at: 3836 4483 6520 3836
Size: 647 2037 4284
Fragments arranged by size:
4284 2037 647
BlpI GC'TnA_GC
Cuts at: 4080 4080
Size: 6968
Bme1580I G_kGCm'C
Cuts at: 1072 1312 2641 4593 4686 5742 1072
Size: 240 1329 1952 93 1056 2298
Fragments arranged by size:
2298 1952 1329 1056 240 93
BmgBI CAC'GTC
Cuts at: 2926 2926
Size: 6968
BmrI ACTGGGnnnn_n'
Cuts at: 1252 1875 4180 4261 4596 6740 1252
Size: 623 2305 81 335 2144 1480
Fragments arranged by size:
2305 2144 1480 623 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5335 5335
Size: 6968
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 578 2111 3136 578
Size: 1533 1025 4410
Fragments arranged by size:
4410 1533 1025
Bpu10I CC'TnA_GC
Cuts at: 2884 3548 2884
Size: 664 6304
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 514 3001 3820 4144 5023 5515 5813 514
Size: 2487 819 324 879 492 298 1669
Fragments arranged by size:
2487 1669 879 819 492 324 298
BsaI GGTCTCn'nnnn_
Cuts at: 6672 6672
Size: 6968
BsaAI yAC'GTr
Cuts at: 3364 4321 3364
Size: 957 6011
BsaBI GATnn'nnATC
Cuts at: 6 4482 6519 6
Size: 4476 2037 455
Fragments arranged by size:
4476 2037 455
BsaHI Gr'CG_yC
Cuts at: 133 1136 4399 4647 5349 6845 133
Size: 1003 3263 248 702 1496 256
Fragments arranged by size:
3263 1496 1003 702 256 248
BsaWI w'CCGG_w
Cuts at: 3243 4157 4678 5356 5630 5777 6188 3243
Size: 914 521 678 274 147 411 4023
Fragments arranged by size:
4023 914 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 764 776 794 806 764
Size: 12 18 12 6926
Fragments arranged by size:
6926 18 12 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2613 2824 2974 3103 3817 2613
Size: 211 150 129 714 5764
Fragments arranged by size:
5764 714 211 150 129
BseYI C'CCAG_C
Cuts at: 1715 3558 4393 5151 5728 6224 1715
Size: 1843 835 758 577 496 2459
Fragments arranged by size:
2459 1843 835 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 924 1126 924
Size: 202 6766
BsiEI CG_ry'CG
Cuts at: 104 4510 4556 5764 104
Size: 4406 46 1208 1308
Fragments arranged by size:
4406 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 530 763 1072 2157 2506 3176 3398 3498 3982
Size: 233 309 1085 349 670 222 100 484
Cuts at: 3982 4760 4950 5742 530
Size: 778 190 792 1756
Fragments arranged by size:
1756 1085 792 778 670 484 349 309
233 222 190 100
BsmI GAATG_Cn'
Cuts at: 3047 3903 3047
Size: 856 6112
BsmBI CGTCTCn'nnnn_
Cuts at: 1817 1817
Size: 6968
Bsp1286I G_dGCh'C
Cuts at: 530 763 1072 1312 2157 2506 2641 3176 3398
Size: 233 309 240 845 349 135 535 222
Cuts at: 3398 3498 3982 4593 4686 4760 4950 5012 5742
Size: 100 484 611 93 74 190 62 730
Cuts at: 5742 530
Size: 1756
Fragments arranged by size:
1756 845 730 611 535 484 349 309
240 233 222 190 135 100 93 74
62
BspEI T'CCGG_A
Cuts at: 6188 6188
Size: 6968
BspHI T'CATG_A
Cuts at: 2241 2414 6144 2241
Size: 173 3730 3065
Fragments arranged by size:
3730 3065 173
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1006 4018 4232 4534 4915 1006
Size: 3012 214 302 381 3059
Fragments arranged by size:
3059 3012 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5260 5314 6798 6963 50
Size: 5210 54 1484 165 55
Fragments arranged by size:
5210 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1542 4880 1542
Size: 3338 3630
BsrFI r'CCGG_y
Cuts at: 571 4966 5147 5356 571
Size: 4395 181 209 2183
Fragments arranged by size:
4395 2183 209 181
BsrGI T'GTAC_A
Cuts at: 2258 2258
Size: 6968
BssSI C'ACGA_G
Cuts at: 1364 2228 5239 5597 1364
Size: 864 3011 358 2735
Fragments arranged by size:
3011 2735 864 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1360 4345 1360
Size: 2985 3983
BstBI TT'CG_AA
Cuts at: 2082 2580 5329 2082
Size: 498 2749 3721
Fragments arranged by size:
3721 2749 498
BstEII G'GTnAC_C
Cuts at: 2218 2218
Size: 6968
BstXI CCAn_nnnn'nTGG
Cuts at: 1674 3700 1674
Size: 2026 4942
BstYI r'GATC_y
Cuts at: 1 809 1029 2295 3836 3993 4460 4483 4818
Size: 808 220 1266 1541 157 467 23 335
Cuts at: 4818 5064 6076 6162 6174 6520 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1541 1266 1012 808 467 449 346 335
246 220 157 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 3148 3148
Size: 6968
BtgI C'CryG_G
Cuts at: 107 1047 3706 107
Size: 940 2659 3369
Fragments arranged by size:
3369 2659 940
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4918 5099 5262 4918
Size: 181 163 6624
Fragments arranged by size:
6624 181 163
BtsI GCAGTG_nn'
Cuts at: 322 401 1868 4211 322
Size: 79 1467 2343 3079
Fragments arranged by size:
3079 2343 1467 79
DraI TTT'AAA
Cuts at: 311 929 2325 3967 311
Size: 618 1396 1642 3312
Fragments arranged by size:
3312 1642 1396 618
DrdI GACnn_nn'nnGTC
Cuts at: 4674 5532 4674
Size: 858 6110
EaeI y'GGCC_r
Cuts at: 2753 3254 4553 4727 5118 5145 6490 2753
Size: 501 1299 174 391 27 1345 3231
Fragments arranged by size:
3231 1345 1299 501 391 174 27
EagI C'GGCC_G
Cuts at: 4553 4553
Size: 6968
EarI CTCTTCn'nnn_
Cuts at: 257 1123 2861 2951 3008 3463 4991 5201 257
Size: 866 1738 90 57 455 1528 210 2024
Fragments arranged by size:
2024 1738 1528 866 455 210 90 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2985 3625 5176 5486 5632 6778 2985
Size: 640 1551 310 146 1146 3175
Fragments arranged by size:
3175 1551 1146 640 310 146
EcoICRI GAG'CTC
Cuts at: 761 3174 3396 3980 761
Size: 2413 222 584 3749
Fragments arranged by size:
3749 2413 584 222
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 416 578 896 1166 1219 1594 2111 2158 2570
Size: 162 318 270 53 375 517 47 412
Cuts at: 2570 3077 3136 3877 4793 5225 5972 6240 416
Size: 507 59 741 916 432 747 268 1144
Fragments arranged by size:
1144 916 747 741 517 507 432 412
375 318 270 268 162 59 53 47
EcoO109I rG'GnC_Cy
Cuts at: 3286 4107 3286
Size: 821 6147
EcoRI G'AATT_C
Cuts at: 3972 3972
Size: 6968
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1126 1279 4987 1126
Size: 153 3708 3107
Fragments arranged by size:
3708 3107 153
FspI TGC'GCA
Cuts at: 4749 4749
Size: 6968
HaeII r_GCGC'y
Cuts at: 136 3208 4402 4650 5672 6651 136
Size: 3072 1194 248 1022 979 453
Fragments arranged by size:
3072 1194 1022 979 453 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2709 2741 3079 3111 3270 3302 4810 4840 4842
Size: 32 338 32 159 32 1508 30 2
Cuts at: 4842 4872 2709
Size: 30 4805
Fragments arranged by size:
4805 1508 338 159 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2366 4007 2366
Size: 1641 5327
HindIII A'AGCT_T
Cuts at: 506 1108 2097 2327 506
Size: 602 989 230 5147
Fragments arranged by size:
5147 989 602 230
HpaI GTT'AAC
Cuts at: 2366 2366
Size: 6968
KasI G'GCGC_C
Cuts at: 132 4398 4646 132
Size: 4266 248 2454
Fragments arranged by size:
4266 2454 248
KpnI G_GTAC'C
Cuts at: 1336 3988 1336
Size: 2652 4316
MfeI C'AATT_G
Cuts at: 197 494 197
Size: 297 6671
MluI A'CGCG_T
Cuts at: 6401 6401
Size: 6968
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 750 5639 5823 750
Size: 4889 184 1895
Fragments arranged by size:
4889 1895 184
MscI TGG'CCA
Cuts at: 2755 3256 4729 2755
Size: 501 1473 4994
Fragments arranged by size:
4994 1473 501
MslI CAynn'nnrTG
Cuts at: 523 749 1075 2032 2563 2773 3698 3781 4957
Size: 226 326 957 531 210 925 83 1176
Cuts at: 4957 5084 523
Size: 127 2407
Fragments arranged by size:
2407 1176 957 925 531 326 226 210
127 83
MspA1I CmG'CkG
Cuts at: 3072 3313 3585 4079 4393 4753 5766 6011 3072
Size: 241 272 494 314 360 1013 245 4029
Fragments arranged by size:
4029 1013 494 360 314 272 245 241
NaeI GCC'GGC
Cuts at: 573 5149 573
Size: 4576 2392
NarI GG'CG_CC
Cuts at: 133 4399 4647 133
Size: 4266 248 2454
Fragments arranged by size:
4266 2454 248
NcoI C'CATG_G
Cuts at: 107 1047 3706 107
Size: 940 2659 3369
Fragments arranged by size:
3369 2659 940
NdeI CA'TA_TG
Cuts at: 372 1913 372
Size: 1541 5427
NgoMIV G'CCGG_C
Cuts at: 571 5147 571
Size: 4576 2392
NspI r_CATG'y
Cuts at: 2259 2477 4021 4521 5428 2259
Size: 218 1544 500 907 3799
Fragments arranged by size:
3799 1544 907 500 218
PciI A'CATG_T
Cuts at: 2255 5424 2255
Size: 3169 3799
PflMI CCAn_nnn'nTGG
Cuts at: 1887 1887
Size: 6968
PmeI GTTT'AAAC
Cuts at: 3967 3967
Size: 6968
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1574 1606 6107 6139 1574
Size: 32 4501 32 2403
Fragments arranged by size:
4501 2403 32 32
PpuMI rG'GwC_Cy
Cuts at: 3286 3286
Size: 6968
PsiI TTA'TAA
Cuts at: 1327 1327
Size: 6968
PstI C_TGCA'G
Cuts at: 261 1746 3950 4015 4700 261
Size: 1485 2204 65 685 2529
Fragments arranged by size:
2529 2204 1485 685 65
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6968
PvuII CAG'CTG
Cuts at: 3072 3313 4393 4753 3072
Size: 241 1080 360 5287
Fragments arranged by size:
5287 1080 360 241
RsrII CG'GwC_CG
Cuts at: 5163 5163
Size: 6968
SacI G_AGCT'C
Cuts at: 763 3176 3398 3982 763
Size: 2413 222 584 3749
Fragments arranged by size:
3749 2413 584 222
SalI G'TCGA_C
Cuts at: 4005 4005
Size: 6968
SapI GCTCTTCn'nnn_
Cuts at: 4991 5201 4991
Size: 210 6758
SbfI CC_TGCA'GG
Cuts at: 4015 4015
Size: 6968
ScaI AGT'ACT
Cuts at: 1492 2088 6485 1492
Size: 596 4397 1975
Fragments arranged by size:
4397 1975 596
SfcI C'TryA_G
Cuts at: 33 143 257 1742 3946 4011 4696 5689 5880
Size: 110 114 1485 2204 65 685 993 191
Cuts at: 5880 33
Size: 1121
Fragments arranged by size:
2204 1485 1121 993 685 191 114 110
65
SfoI GGC'GCC
Cuts at: 134 4400 4648 134
Size: 4266 248 2454
Fragments arranged by size:
4266 2454 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6968
SmaI CCC'GGG
Cuts at: 3990 3990
Size: 6968
SmlI C'TyrA_G
Cuts at: 529 1009 2980 3799 4123 5038 5530 5792 529
Size: 480 1971 819 324 915 492 262 1705
Fragments arranged by size:
1971 1705 915 819 492 480 324 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6968
SphI G_CATG'C
Cuts at: 4021 4521 4021
Size: 500 6468
SspI AAT'ATT
Cuts at: 168 1155 1228 168
Size: 987 73 5908
Fragments arranged by size:
5908 987 73
StyI C'CwwG_G
Cuts at: 107 1047 1146 3706 4102 4456 107
Size: 940 99 2560 396 354 2619
Fragments arranged by size:
2619 2560 940 396 354 99
SwaI ATTT'AAAT
Cuts at: 311 311
Size: 6968
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3762 4551 4684 5143 5352 3762
Size: 789 133 459 209 5378
Fragments arranged by size:
5378 789 459 209 133
TatI w'GTAC_w
Cuts at: 1490 2086 2258 3294 3650 6483 1490
Size: 596 172 1036 356 2833 1975
Fragments arranged by size:
2833 1975 1036 596 356 172
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 776 808 3813 3845 6485 6517 776
Size: 32 3005 32 2640 32 1227
Fragments arranged by size:
3005 2640 1227 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4765 4765
Size: 6968
XbaI T'CTAG_A
Cuts at: 3999 3999
Size: 6968
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3756 4463 3756
Size: 707 6261
XmaI C'CCGG_G
Cuts at: 3988 3988
Size: 6968
XmnI GAAnn'nnTTC
Cuts at: 893 1544 1568 3419 893
Size: 651 24 1851 4442
Fragments arranged by size:
4442 1851 651 24
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PciI PflMI
PmeI PpiI PpuMI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BmtI BsiWI BssHII BstZ17I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PshAI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI XhoI ZraI