Restriction map for
pF1KA0394
(Circular) (Six-base) MAPSORT of: pF1KA0394.seq Check: 8109 from: 1 to: 4349
pF1KA0394 4349 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 1387 1387
Size: 4349
Acc65I G'GTAC_C
Cuts at: 1365 1365
Size: 4349
AclI AA'CG_TT
Cuts at: 4189 4189
Size: 4349
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 619 2174 2606 3353 3621 619
Size: 1555 432 747 268 1347
Fragments arranged by size:
1555 1347 747 432 268
AflIII A'CryG_T
Cuts at: 1217 1701 2805 3782 1217
Size: 484 1104 977 1784
Fragments arranged by size:
1784 1104 977 484
AgeI A'CCGG_T
Cuts at: 373 2737 373
Size: 2364 1985
AleI CACnn'nnGTG
Cuts at: 356 356
Size: 4349
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 628 628
Size: 4349
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 379 411 569 601 379
Size: 32 158 32 4127
Fragments arranged by size:
4127 158 32 32
AlwNI CAG_nnn'CTG
Cuts at: 346 390 489 492 984 1182 1654 3221 346
Size: 44 99 3 492 198 472 1567 1474
Fragments arranged by size:
1567 1474 492 472 198 99 44 3
ApaI G_GGCC'C
Cuts at: 744 744
Size: 4349
ApaLI G'TGCA_C
Cuts at: 3119 3119
Size: 4349
ApoI r'AATT_y
Cuts at: 631 1353 3702 3958 631
Size: 722 2349 256 1022
Fragments arranged by size:
2349 1022 722 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4349
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4349
AvaI C'yCGr_G
Cuts at: 1369 1369
Size: 4349
BamHI G'GATC_C
Cuts at: 394 1374 394
Size: 980 3369
BanI G'GyrC_C
Cuts at: 1365 1779 2027 2062 1365
Size: 414 248 35 3652
Fragments arranged by size:
3652 414 248 35
BanII G_rGCy'C
Cuts at: 286 744 1244 1363 2393 286
Size: 458 500 119 1030 2242
Fragments arranged by size:
2242 1030 500 458 119
BbeI G_GCGC'C
Cuts at: 1783 2031 1783
Size: 248 4101
BbsI GAAGACnn'nnnn_
Cuts at: 668 979 668
Size: 311 4038
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 882 916 882
Size: 34 4315
BciVI GTATCCnnnnn_n'
Cuts at: 301 2247 3008 4349 301
Size: 1946 761 1341 301
Fragments arranged by size:
1946 1341 761 301
BclI T'GATC_A
Cuts at: 1869 1869
Size: 4349
BglII A'GATC_T
Cuts at: 1864 3901 1864
Size: 2037 2312
BlpI GC'TnA_GC
Cuts at: 683 997 1461 683
Size: 314 464 3571
Fragments arranged by size:
3571 464 314
Bme1580I G_kGCm'C
Cuts at: 744 1974 2067 3123 744
Size: 1230 93 1056 1970
Fragments arranged by size:
1970 1230 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1089 1561 1642 1977 4121 1089
Size: 472 81 335 2144 1317
Fragments arranged by size:
2144 1317 472 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 738 1078 2716 738
Size: 340 1638 2371
Fragments arranged by size:
2371 1638 340
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 323 571 985 1006 1073 1168 323
Size: 248 414 21 67 95 3504
Fragments arranged by size:
3504 414 248 95 67 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 769 1525 2404 2896 3194 769
Size: 756 879 492 298 1924
Fragments arranged by size:
1924 879 756 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 214 953 4053 214
Size: 739 3100 510
Fragments arranged by size:
3100 739 510
BsaAI yAC'GTr
Cuts at: 1702 1702
Size: 4349
BsaBI GATnn'nnATC
Cuts at: 6 399 1863 3900 6
Size: 393 1464 2037 455
Fragments arranged by size:
2037 1464 455 393
BsaHI Gr'CG_yC
Cuts at: 1780 2028 2730 4226 1780
Size: 248 702 1496 1903
Fragments arranged by size:
1903 1496 702 248
BsaWI w'CCGG_w
Cuts at: 373 1538 2059 2737 3011 3158 3569 373
Size: 1165 521 678 274 147 411 1153
Fragments arranged by size:
1165 1153 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 569 599 569
Size: 30 4319
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1027 1027
Size: 4349
BseYI C'CCAG_C
Cuts at: 238 327 568 977 1774 2532 3109 3605 238
Size: 89 241 409 797 758 577 496 982
Fragments arranged by size:
982 797 758 577 496 409 241 89
BsiEI CG_ry'CG
Cuts at: 104 1891 1937 3145 104
Size: 1787 46 1208 1308
Fragments arranged by size:
1787 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 286 1307 1363 2141 2331 3123 286
Size: 1021 56 778 190 792 1512
Fragments arranged by size:
1512 1021 792 778 190 56
BsmBI CGTCTCn'nnnn_
Cuts at: 1049 1049
Size: 4349
Bsp1286I G_dGCh'C
Cuts at: 286 744 1244 1307 1363 1974 2067 2141 2331
Size: 458 500 63 56 611 93 74 190
Cuts at: 2331 2393 3123 286
Size: 62 730 1512
Fragments arranged by size:
1512 730 611 500 458 190 93 74
63 62 56
BspEI T'CCGG_A
Cuts at: 3569 3569
Size: 4349
BspHI T'CATG_A
Cuts at: 3525 3525
Size: 4349
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1399 1613 1915 2296 1399
Size: 214 302 381 3452
Fragments arranged by size:
3452 381 302 214
BsrBI CCG'CTC
Cuts at: 50 951 1143 2641 2695 4179 4344 50
Size: 901 192 1498 54 1484 165 55
Fragments arranged by size:
1498 1484 901 192 165 55 54
BsrDI GCAATG_nn'
Cuts at: 878 2261 878
Size: 1383 2966
BsrFI r'CCGG_y
Cuts at: 188 373 2347 2528 2737 188
Size: 185 1974 181 209 1800
Fragments arranged by size:
1974 1800 209 185 181
BssSI C'ACGA_G
Cuts at: 2620 2978 2620
Size: 358 3991
BstAPI GCAn_nnn'nTGC
Cuts at: 1726 1726
Size: 4349
BstBI TT'CG_AA
Cuts at: 1260 2710 1260
Size: 1450 2899
BstEII G'GTnAC_C
Cuts at: 1119 1119
Size: 4349
BstXI CCAn_nnnn'nTGG
Cuts at: 161 506 1131 161
Size: 345 625 3379
Fragments arranged by size:
3379 625 345
BstYI r'GATC_y
Cuts at: 1 394 1077 1374 1841 1864 2199 2445 3457
Size: 393 683 297 467 23 335 246 1012
Cuts at: 3457 3543 3555 3901 1
Size: 86 12 346 449
Fragments arranged by size:
1012 683 467 449 393 346 335 297
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 182 182
Size: 4349
BtgI C'CryG_G
Cuts at: 107 1244 107
Size: 1137 3212
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 386 2299 2480 2643 386
Size: 1913 181 163 2092
Fragments arranged by size:
2092 1913 181 163
BtsI GCAGTG_nn'
Cuts at: 1592 1592
Size: 4349
DraI TTT'AAA
Cuts at: 1348 1348
Size: 4349
DrdI GACnn_nn'nnGTC
Cuts at: 2055 2913 2055
Size: 858 3491
EaeI y'GGCC_r
Cuts at: 1274 1934 2108 2499 2526 3871 1274
Size: 660 174 391 27 1345 1752
Fragments arranged by size:
1752 1345 660 391 174 27
EagI C'GGCC_G
Cuts at: 1934 1934
Size: 4349
EarI CTCTTCn'nnn_
Cuts at: 748 1106 2372 2582 748
Size: 358 1266 210 2515
Fragments arranged by size:
2515 1266 358 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 110 778 1312 2557 2867 3013 4159 110
Size: 668 534 1245 310 146 1146 300
Fragments arranged by size:
1245 1146 668 534 310 300 146
EcoICRI GAG'CTC
Cuts at: 284 1361 284
Size: 1077 3272
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 323 571 619 985 1006 1073 1168 2174 2606
Size: 248 48 366 21 67 95 1006 432
Cuts at: 2606 3353 3621 323
Size: 747 268 1051
Fragments arranged by size:
1051 1006 747 432 366 268 248 95
67 48 21
EcoO109I rG'GnC_Cy
Cuts at: 323 543 1488 323
Size: 220 945 3184
Fragments arranged by size:
3184 945 220
EcoRI G'AATT_C
Cuts at: 631 1353 631
Size: 722 3627
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2368 2368
Size: 4349
FspI TGC'GCA
Cuts at: 2130 2130
Size: 4349
HaeII r_GCGC'y
Cuts at: 1783 2031 3053 4032 1783
Size: 248 1022 979 2100
Fragments arranged by size:
2100 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 444 476 2191 2221 2223 2253 444
Size: 32 1715 30 2 30 2540
Fragments arranged by size:
2540 1715 32 30 30 2
HincII GTy'rAC
Cuts at: 204 1388 204
Size: 1184 3165
HindIII A'AGCT_T
Cuts at: 799 799
Size: 4349
KasI G'GCGC_C
Cuts at: 1779 2027 1779
Size: 248 4101
KpnI G_GTAC'C
Cuts at: 1369 1369
Size: 4349
MluI A'CGCG_T
Cuts at: 3782 3782
Size: 4349
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 319 3020 3204 319
Size: 2701 184 1464
Fragments arranged by size:
2701 1464 184
MscI TGG'CCA
Cuts at: 2110 2110
Size: 4349
MslI CAynn'nnrTG
Cuts at: 356 2338 2465 356
Size: 1982 127 2240
Fragments arranged by size:
2240 1982 127
MspA1I CmG'CkG
Cuts at: 489 597 984 1200 1254 1460 1774 2134 3147
Size: 108 387 216 54 206 314 360 1013
Cuts at: 3147 3392 489
Size: 245 1446
Fragments arranged by size:
1446 1013 387 360 314 245 216 206
108 54
NaeI GCC'GGC
Cuts at: 190 2530 190
Size: 2340 2009
NarI GG'CG_CC
Cuts at: 1780 2028 1780
Size: 248 4101
NcoI C'CATG_G
Cuts at: 107 107
Size: 4349
NgoMIV G'CCGG_C
Cuts at: 188 2528 188
Size: 2340 2009
NspI r_CATG'y
Cuts at: 1221 1402 1902 2809 1221
Size: 181 500 907 2761
Fragments arranged by size:
2761 907 500 181
PciI A'CATG_T
Cuts at: 1217 2805 1217
Size: 1588 2761
PflMI CCAn_nnn'nTGG
Cuts at: 390 984 390
Size: 594 3755
PfoI T'CCnGG_A
Cuts at: 638 638
Size: 4349
PmeI GTTT'AAAC
Cuts at: 1348 1348
Size: 4349
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 569 601 3488 3520 569
Size: 32 2887 32 1398
Fragments arranged by size:
2887 1398 32 32
PpuMI rG'GwC_Cy
Cuts at: 323 543 323
Size: 220 4129
PspOMI G'GGCC_C
Cuts at: 740 740
Size: 4349
PstI C_TGCA'G
Cuts at: 1396 2081 1396
Size: 685 3664
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4349
PvuII CAG'CTG
Cuts at: 489 597 984 1200 1254 1774 2134 489
Size: 108 387 216 54 520 360 2704
Fragments arranged by size:
2704 520 387 360 216 108 54
RsrII CG'GwC_CG
Cuts at: 2544 2544
Size: 4349
SacI G_AGCT'C
Cuts at: 286 1363 286
Size: 1077 3272
SalI G'TCGA_C
Cuts at: 1386 1386
Size: 4349
SanDI GG'GwC_CC
Cuts at: 323 323
Size: 4349
SapI GCTCTTCn'nnn_
Cuts at: 748 2372 2582 748
Size: 1624 210 2515
Fragments arranged by size:
2515 1624 210
SbfI CC_TGCA'GG
Cuts at: 1396 1396
Size: 4349
ScaI AGT'ACT
Cuts at: 3866 3866
Size: 4349
SfcI C'TryA_G
Cuts at: 33 1392 2077 3070 3261 33
Size: 1359 685 993 191 1121
Fragments arranged by size:
1359 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1781 2029 1781
Size: 248 4101
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4349
SgrAI Cr'CCGG_yG
Cuts at: 373 373
Size: 4349
SmaI CCC'GGG
Cuts at: 1371 1371
Size: 4349
SmlI C'TyrA_G
Cuts at: 784 1504 2419 2911 3173 784
Size: 720 915 492 262 1960
Fragments arranged by size:
1960 915 720 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4349
SphI G_CATG'C
Cuts at: 1402 1902 1402
Size: 500 3849
StyI C'CwwG_G
Cuts at: 107 549 701 725 1483 1837 107
Size: 442 152 24 758 354 2619
Fragments arranged by size:
2619 758 442 354 152 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 479 1932 2065 2524 2733 479
Size: 1453 133 459 209 2095
Fragments arranged by size:
2095 1453 459 209 133
TatI w'GTAC_w
Cuts at: 1190 3864 1190
Size: 2674 1675
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3866 3898 3866
Size: 32 4317
Tth111I GACn'n_nGTC
Cuts at: 2146 2146
Size: 4349
XbaI T'CTAG_A
Cuts at: 1380 1380
Size: 4349
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1844 1844
Size: 4349
XmaI C'CCGG_G
Cuts at: 1369 1369
Size: 4349
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AscI AvrII BaeI
BbvCI BfrBI BglI BmgBI BmtI Bpu10I BsgI BsiWI
BsmI BsrGI BssHII BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PmlI PshAI PsiI PspXI
PsrI SacII SexAI SfiI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI