Restriction map for
pF1KA0397
(Circular) (Six-base) MAPSORT of: pF1KA0397.seq Check: 7772 from: 1 to: 6131
pF1KA0397 6131 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1193 1878 1911 1193
Size: 685 33 5413
Fragments arranged by size:
5413 685 33
AatII G_ACGT'C
Cuts at: 1832 2573 1832
Size: 741 5390
AccI GT'mk_AC
Cuts at: 3169 3169
Size: 6131
Acc65I G'GTAC_C
Cuts at: 1913 3147 1913
Size: 1234 4897
AclI AA'CG_TT
Cuts at: 5971 5971
Size: 6131
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1000 1056 2141 2670 3956 4388 5135 5403 1000
Size: 56 1085 529 1286 432 747 268 1728
Fragments arranged by size:
1728 1286 1085 747 529 432 268 56
AflIII A'CryG_T
Cuts at: 492 1730 2585 3483 4587 5564 492
Size: 1238 855 898 1104 977 1059
Fragments arranged by size:
1238 1104 1059 977 898 855
AgeI A'CCGG_T
Cuts at: 1547 4519 1547
Size: 2972 3159
AleI CACnn'nnGTG
Cuts at: 328 1123 2176 328
Size: 795 1053 4283
Fragments arranged by size:
4283 1053 795
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 977 1141 3074 977
Size: 164 1933 4034
Fragments arranged by size:
4034 1933 164
AlwNI CAG_nnn'CTG
Cuts at: 381 601 1529 2778 3436 5003 381
Size: 220 928 1249 658 1567 1509
Fragments arranged by size:
1567 1509 1249 928 658 220
ApaI G_GGCC'C
Cuts at: 1433 2342 1433
Size: 909 5222
ApaLI G'TGCA_C
Cuts at: 1669 4901 1669
Size: 3232 2899
ApoI r'AATT_y
Cuts at: 3135 5484 5740 3135
Size: 2349 256 3526
Fragments arranged by size:
3526 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6131
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6131
AvaI C'yCGr_G
Cuts at: 1226 1598 1701 2158 2256 2554 3083 3151 1226
Size: 372 103 457 98 298 529 68 4206
Fragments arranged by size:
4206 529 457 372 298 103 98 68
BamHI G'GATC_C
Cuts at: 779 2376 3156 779
Size: 1597 780 3754
Fragments arranged by size:
3754 1597 780
BanI G'GyrC_C
Cuts at: 719 1065 1448 1913 2170 2637 2731 3147 3561
Size: 346 383 465 257 467 94 416 414
Cuts at: 3561 3809 3844 719
Size: 248 35 3006
Fragments arranged by size:
3006 467 465 416 414 383 346 257
248 94 35
BanII G_rGCy'C
Cuts at: 448 1244 1433 1460 1477 2111 2342 2372 3145
Size: 796 189 27 17 634 231 30 773
Cuts at: 3145 4175 448
Size: 1030 2404
Fragments arranged by size:
2404 1030 796 773 634 231 189 30
27 17
BbeI G_GCGC'C
Cuts at: 2641 3565 3813 2641
Size: 924 248 4959
Fragments arranged by size:
4959 924 248
BbsI GAAGACnn'nnnn_
Cuts at: 218 343 218
Size: 125 6006
BbvCI CC'TCA_GC
Cuts at: 472 472
Size: 6131
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2001 2035 2001
Size: 34 6097
BciVI GTATCCnnnnn_n'
Cuts at: 4029 4790 6131 4029
Size: 761 1341 4029
Fragments arranged by size:
4029 1341 761
BclI T'GATC_A
Cuts at: 2664 3651 2664
Size: 987 5144
BfrBI ATG'CAT
Cuts at: 2805 2805
Size: 6131
BglI GCCn_nnn'nGGC
Cuts at: 1142 1446 1560 1142
Size: 304 114 5713
Fragments arranged by size:
5713 304 114
BglII A'GATC_T
Cuts at: 3646 5683 3646
Size: 2037 4094
BlpI GC'TnA_GC
Cuts at: 447 3047 3243 447
Size: 2600 196 3335
Fragments arranged by size:
3335 2600 196
Bme1580I G_kGCm'C
Cuts at: 346 837 1070 1336 1433 1673 2173 2342 3756
Size: 491 233 266 97 240 500 169 1414
Cuts at: 3756 3849 4905 346
Size: 93 1056 1572
Fragments arranged by size:
1572 1414 1056 500 491 266 240 233
169 97 93
BmgBI CAC'GTC
Cuts at: 341 1731 2604 341
Size: 1390 873 3868
Fragments arranged by size:
3868 1390 873
BmrI ACTGGGnnnn_n'
Cuts at: 340 1056 3343 3424 3759 5903 340
Size: 716 2287 81 335 2144 568
Fragments arranged by size:
2287 2144 716 568 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 992 2226 2346 4498 992
Size: 1234 120 2152 2625
Fragments arranged by size:
2625 2152 1234 120
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1228 1474 1482 1795 2198 2316 2431 1228
Size: 246 8 313 403 118 115 4928
Fragments arranged by size:
4928 403 313 246 118 115 8
Bpu10I CC'TnA_GC
Cuts at: 472 1558 2141 472
Size: 1086 583 4462
Fragments arranged by size:
4462 1086 583
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 649 780 3307 4186 4678 4976 649
Size: 131 2527 879 492 298 1804
Fragments arranged by size:
2527 1804 879 492 298 131
BsaI GGTCTCn'nnnn_
Cuts at: 5835 5835
Size: 6131
BsaAI yAC'GTr
Cuts at: 495 900 2031 2586 3484 495
Size: 405 1131 555 898 3142
Fragments arranged by size:
3142 1131 898 555 405
BsaBI GATnn'nnATC
Cuts at: 6 3645 5682 6
Size: 3639 2037 455
Fragments arranged by size:
3639 2037 455
BsaHI Gr'CG_yC
Cuts at: 276 1829 2570 2638 3562 3810 4512 6008 276
Size: 1553 741 68 924 248 702 1496 399
Fragments arranged by size:
1553 1496 924 741 702 399 248 68
BsaWI w'CCGG_w
Cuts at: 782 1361 1547 3320 3841 4519 4793 4940 5351
Size: 579 186 1773 521 678 274 147 411
Cuts at: 5351 782
Size: 1562
Fragments arranged by size:
1773 1562 678 579 521 411 274 186
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1447 1477 2038 2068 2746 2776 1447
Size: 30 561 30 678 30 4802
Fragments arranged by size:
4802 678 561 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 196 1339 1467 1485 1995 2223 2320 2437 196
Size: 1143 128 18 510 228 97 117 3890
Fragments arranged by size:
3890 1143 510 228 128 117 97 18
BseYI C'CCAG_C
Cuts at: 768 1256 1432 1522 1983 2380 3556 4314 4891
Size: 488 176 90 461 397 1176 758 577
Cuts at: 4891 5387 768
Size: 496 1512
Fragments arranged by size:
1512 1176 758 577 496 488 461 397
176 90
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 263 841 928 937 1485 2053 2473 2530 2598
Size: 578 87 9 548 568 420 57 68
Cuts at: 2598 2635 3124 263
Size: 37 489 3270
Fragments arranged by size:
3270 578 568 548 489 420 87 68
57 37 9
BsiEI CG_ry'CG
Cuts at: 104 1548 3673 3719 4927 104
Size: 1444 2125 46 1208 1308
Fragments arranged by size:
2125 1444 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1394 1673 1814 2597 2942 3145 3923 4113 4905
Size: 279 141 783 345 203 778 190 792
Cuts at: 4905 1394
Size: 2620
Fragments arranged by size:
2620 792 783 778 345 279 203 190
141
BsmI GAATG_Cn'
Cuts at: 1304 2193 1304
Size: 889 5242
BsmBI CGTCTCn'nnnn_
Cuts at: 267 2561 267
Size: 2294 3837
Bsp1286I G_dGCh'C
Cuts at: 346 448 837 1070 1244 1336 1394 1433 1460
Size: 102 389 233 174 92 58 39 27
Cuts at: 1460 1477 1673 1814 2111 2173 2342 2372 2597
Size: 17 196 141 297 62 169 30 225
Cuts at: 2597 2942 3145 3756 3849 3923 4113 4175 4905
Size: 345 203 611 93 74 190 62 730
Cuts at: 4905 346
Size: 1572
Fragments arranged by size:
1572 730 611 389 345 297 233 225
203 196 190 174 169 141 102 93
92 74 62 62 58 39 30 27
17
BspEI T'CCGG_A
Cuts at: 782 1361 5351 782
Size: 579 3990 1562
Fragments arranged by size:
3990 1562 579
BspHI T'CATG_A
Cuts at: 1001 2696 5307 1001
Size: 1695 2611 1825
Fragments arranged by size:
2611 1825 1695
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1193 1878 1911 2495 3181 3395 3697 4078 1193
Size: 685 33 584 686 214 302 381 3246
Fragments arranged by size:
3246 686 685 584 381 302 214 33
BsrBI CCG'CTC
Cuts at: 50 1112 1557 1971 4423 4477 5961 6126 50
Size: 1062 445 414 2452 54 1484 165 55
Fragments arranged by size:
2452 1484 1062 445 414 165 55 54
BsrDI GCAATG_nn'
Cuts at: 227 4043 227
Size: 3816 2315
BsrFI r'CCGG_y
Cuts at: 1547 1694 4129 4310 4519 1547
Size: 147 2435 181 209 3159
Fragments arranged by size:
3159 2435 209 181 147
BssSI C'ACGA_G
Cuts at: 1060 1387 1813 4402 4760 1060
Size: 327 426 2589 358 2431
Fragments arranged by size:
2589 2431 426 358 327
BstAPI GCAn_nnn'nTGC
Cuts at: 1094 3508 1094
Size: 2414 3717
BstBI TT'CG_AA
Cuts at: 2300 4492 2300
Size: 2192 3939
BstEII G'GTnAC_C
Cuts at: 2649 2649
Size: 6131
BstYI r'GATC_y
Cuts at: 1 779 1817 2376 2774 3068 3156 3623 3646
Size: 778 1038 559 398 294 88 467 23
Cuts at: 3646 3981 4227 5239 5325 5337 5683 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1038 1012 778 559 467 449 398 346
335 294 246 88 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 411 479 2007 2396 411
Size: 68 1528 389 4146
Fragments arranged by size:
4146 1528 389 68
BtgI C'CryG_G
Cuts at: 107 1313 1640 1712 2177 2198 2249 2735 107
Size: 1206 327 72 465 21 51 486 3503
Fragments arranged by size:
3503 1206 486 465 327 72 51 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2940 4081 4262 4425 2940
Size: 1141 181 163 4646
Fragments arranged by size:
4646 1141 181 163
BtsI GCAGTG_nn'
Cuts at: 251 306 586 1092 1623 1904 3374 251
Size: 55 280 506 531 281 1470 3008
Fragments arranged by size:
3008 1470 531 506 281 280 55
DraI TTT'AAA
Cuts at: 3130 3130
Size: 6131
DraIII CAC_nnn'GTG
Cuts at: 3102 3102
Size: 6131
DrdI GACnn_nn'nnGTC
Cuts at: 1656 3837 4695 1656
Size: 2181 858 3092
Fragments arranged by size:
3092 2181 858
EaeI y'GGCC_r
Cuts at: 1377 1848 3716 3890 4281 4308 5653 1377
Size: 471 1868 174 391 27 1345 1855
Fragments arranged by size:
1868 1855 1345 471 391 174 27
EagI C'GGCC_G
Cuts at: 3716 3716
Size: 6131
EarI CTCTTCn'nnn_
Cuts at: 632 1576 2297 2695 4154 4364 632
Size: 944 721 398 1459 210 2399
Fragments arranged by size:
2399 1459 944 721 398 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 568 4339 4649 4795 5941 568
Size: 3771 310 146 1146 758
Fragments arranged by size:
3771 1146 758 310 146
EcoICRI GAG'CTC
Cuts at: 3143 3143
Size: 6131
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1000 1056 1228 1474 1482 1795 2141 2198 2316
Size: 56 172 246 8 313 346 57 118
Cuts at: 2316 2431 2670 3956 4388 5135 5403 1000
Size: 115 239 1286 432 747 268 1728
Fragments arranged by size:
1728 1286 747 432 346 313 268 246
239 172 118 115 57 56 8
EcoNI CCTnn'n_nnAGG
Cuts at: 1723 2278 2971 1723
Size: 555 693 4883
Fragments arranged by size:
4883 693 555
EcoO109I rG'GnC_Cy
Cuts at: 429 462 637 1706 2115 2155 3087 3270 429
Size: 33 175 1069 409 40 932 183 3290
Fragments arranged by size:
3290 1069 932 409 183 175 40 33
EcoRI G'AATT_C
Cuts at: 3135 3135
Size: 6131
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4150 4150
Size: 6131
FspI TGC'GCA
Cuts at: 298 3912 298
Size: 3614 2517
HaeII r_GCGC'y
Cuts at: 514 768 1666 2040 2641 3565 3813 4835 5814
Size: 254 898 374 601 924 248 1022 979
Cuts at: 5814 514
Size: 831
Fragments arranged by size:
1022 979 924 898 831 601 374 254
248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 660 692 1728 1760 2975 3007 3973 4003 4005
Size: 32 1036 32 1215 32 966 30 2
Cuts at: 4005 4035 660
Size: 30 2756
Fragments arranged by size:
2756 1215 1036 966 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 3170 3170
Size: 6131
KasI G'GCGC_C
Cuts at: 2637 3561 3809 2637
Size: 924 248 4959
Fragments arranged by size:
4959 924 248
KpnI G_GTAC'C
Cuts at: 1917 3151 1917
Size: 1234 4897
MluI A'CGCG_T
Cuts at: 5564 5564
Size: 6131
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 791 4802 4986 791
Size: 4011 184 1936
Fragments arranged by size:
4011 1936 184
MscI TGG'CCA
Cuts at: 1850 3892 1850
Size: 2042 4089
MslI CAynn'nnrTG
Cuts at: 328 1123 2176 2810 3056 4120 4247 328
Size: 795 1053 634 246 1064 127 2212
Fragments arranged by size:
2212 1064 1053 795 634 246 127
MspA1I CmG'CkG
Cuts at: 270 286 455 717 1161 1248 1714 1965 2670
Size: 16 169 262 444 87 466 251 705
Cuts at: 2670 2681 3242 3556 3916 4929 5174 270
Size: 11 561 314 360 1013 245 1227
Fragments arranged by size:
1227 1013 705 561 466 444 360 314
262 251 245 169 87 16 11
NaeI GCC'GGC
Cuts at: 4312 4312
Size: 6131
NarI GG'CG_CC
Cuts at: 2638 3562 3810 2638
Size: 924 248 4959
Fragments arranged by size:
4959 924 248
NcoI C'CATG_G
Cuts at: 107 2735 107
Size: 2628 3503
NgoMIV G'CCGG_C
Cuts at: 4310 4310
Size: 6131
NsiI A_TGCA'T
Cuts at: 2807 2807
Size: 6131
NspI r_CATG'y
Cuts at: 1516 2625 2760 3184 3684 4591 1516
Size: 1109 135 424 500 907 3056
Fragments arranged by size:
3056 1109 907 500 424 135
PasI CC'CwG_GG
Cuts at: 774 774
Size: 6131
PciI A'CATG_T
Cuts at: 4587 4587
Size: 6131
PflMI CCAn_nnn'nTGG
Cuts at: 903 2778 903
Size: 1875 4256
PfoI T'CCnGG_A
Cuts at: 2777 2777
Size: 6131
PmeI GTTT'AAAC
Cuts at: 3130 3130
Size: 6131
PmlI CAC'GTG
Cuts at: 900 2031 900
Size: 1131 5000
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5270 5302 5270
Size: 32 6099
PpuMI rG'GwC_Cy
Cuts at: 637 2115 2155 3087 637
Size: 1478 40 932 3681
Fragments arranged by size:
3681 1478 932 40
PspOMI G'GGCC_C
Cuts at: 1429 2338 1429
Size: 909 5222
PstI C_TGCA'G
Cuts at: 386 565 966 2352 3178 3863 386
Size: 179 401 1386 826 685 2654
Fragments arranged by size:
2654 1386 826 685 401 179
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6131
PvuII CAG'CTG
Cuts at: 270 2670 2681 3556 3916 270
Size: 2400 11 875 360 2485
Fragments arranged by size:
2485 2400 875 360 11
RsrII CG'GwC_CG
Cuts at: 4326 4326
Size: 6131
SacI G_AGCT'C
Cuts at: 3145 3145
Size: 6131
SacII CC_GC'GG
Cuts at: 1715 1715
Size: 6131
SalI G'TCGA_C
Cuts at: 3168 3168
Size: 6131
SanDI GG'GwC_CC
Cuts at: 637 637
Size: 6131
SapI GCTCTTCn'nnn_
Cuts at: 4154 4364 4154
Size: 210 5921
SbfI CC_TGCA'GG
Cuts at: 3178 3178
Size: 6131
ScaI AGT'ACT
Cuts at: 571 5648 571
Size: 5077 1054
SfcI C'TryA_G
Cuts at: 33 382 561 962 1530 2348 2456 2676 3174
Size: 349 179 401 568 818 108 220 498
Cuts at: 3174 3859 4852 5043 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 818 685 568 498 401 349
220 191 179 108
SfoI GGC'GCC
Cuts at: 2639 3563 3811 2639
Size: 924 248 4959
Fragments arranged by size:
4959 924 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6131
SmaI CCC'GGG
Cuts at: 1600 1703 2160 3085 3153 1600
Size: 103 457 925 68 4578
Fragments arranged by size:
4578 925 457 103 68
SmlI C'TyrA_G
Cuts at: 664 795 2554 3286 4201 4693 4955 664
Size: 131 1759 732 915 492 262 1840
Fragments arranged by size:
1840 1759 915 732 492 262 131
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6131
SphI G_CATG'C
Cuts at: 3184 3684 3184
Size: 500 5631
StuI AGG'CCT
Cuts at: 484 1942 2977 484
Size: 1458 1035 3638
Fragments arranged by size:
3638 1458 1035
StyI C'CwwG_G
Cuts at: 107 326 647 1724 2735 3265 3619 107
Size: 219 321 1077 1011 530 354 2619
Fragments arranged by size:
2619 1077 1011 530 354 321 219
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1997 2758 2839 3714 3847 4306 4515 1997
Size: 761 81 875 133 459 209 3613
Fragments arranged by size:
3613 875 761 459 209 133 81
TatI w'GTAC_w
Cuts at: 569 5646 569
Size: 5077 1054
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1174 1206 1447 1479 2038 2070 5648 5680 1174
Size: 32 241 32 559 32 3578 32 1625
Fragments arranged by size:
3578 1625 559 241 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 127 535 3091 3928 127
Size: 408 2556 837 2330
Fragments arranged by size:
2556 2330 837 408
XbaI T'CTAG_A
Cuts at: 3162 3162
Size: 6131
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1003 3626 1003
Size: 2623 3508
XhoI C'TCGA_G
Cuts at: 2554 2554
Size: 6131
XmaI C'CCGG_G
Cuts at: 1598 1701 2158 3083 3151 1598
Size: 103 457 925 68 4578
Fragments arranged by size:
4578 925 457 103 68
XmnI GAAnn'nnTTC
Cuts at: 1276 1276
Size: 6131
ZraI GAC'GTC
Cuts at: 1830 2571 1830
Size: 741 5390
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AloI AscI AvrII BaeI BmtI
BsiWI BsrGI BssHII BstXI BstZ17I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI PacI PshAI PsiI PspXI PsrI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI