Restriction map for
pF1KA0399
(Circular) (Six-base) MAPSORT of: pF1KA0399.seq Check: 9011 from: 1 to: 11996
pF1KA0399 11996 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1044 6249 6665 1044
Size: 5205 416 6375
Fragments arranged by size:
6375 5205 416
AatII G_ACGT'C
Cuts at: 4787 4787
Size: 11996
AccI GT'mk_AC
Cuts at: 6563 9034 6563
Size: 2471 9525
Acc65I G'GTAC_C
Cuts at: 2210 9012 2210
Size: 6802 5194
AclI AA'CG_TT
Cuts at: 11836 11836
Size: 11996
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 784 1075 1687 1913 1975 2302 3064 3322
Size: 249 291 612 226 62 327 762 258
Cuts at: 3322 3852 4559 4667 4798 4903 6299 6838 7219
Size: 530 707 108 131 105 1396 539 381
Cuts at: 7219 7270 8364 9821 10253 11000 11268 535
Size: 51 1094 1457 432 747 268 1263
Fragments arranged by size:
1457 1396 1263 1094 762 747 707 612
539 530 432 381 327 291 268 258
249 226 131 108 105 62 51
AfeI AGC'GCT
Cuts at: 236 2179 236
Size: 1943 10053
AflIII A'CryG_T
Cuts at: 512 737 4628 5408 9348 10452 11429 512
Size: 225 3891 780 3940 1104 977 1079
Fragments arranged by size:
3940 3891 1104 1079 977 780 225
AgeI A'CCGG_T
Cuts at: 6039 10384 6039
Size: 4345 7651
AleI CACnn'nnGTG
Cuts at: 131 2610 2612 4823 131
Size: 2479 2 2211 7304
Fragments arranged by size:
7304 2479 2211 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1637 4380 6365 7859 8942 1637
Size: 2743 1985 1494 1083 4691
Fragments arranged by size:
4691 2743 1985 1494 1083
AlwNI CAG_nnn'CTG
Cuts at: 956 1024 1066 1665 2784 3111 4003 6277 7806
Size: 68 42 599 1119 327 892 2274 1529
Cuts at: 7806 8916 9301 10868 956
Size: 1110 385 1567 2084
Fragments arranged by size:
2274 2084 1567 1529 1119 1110 892 599
385 327 68 42
ApaI G_GGCC'C
Cuts at: 175 2964 6418 7059 175
Size: 2789 3454 641 5112
Fragments arranged by size:
5112 3454 2789 641
ApaLI G'TGCA_C
Cuts at: 741 1108 1587 2255 2808 10766 741
Size: 367 479 668 553 7958 1971
Fragments arranged by size:
7958 1971 668 553 479 367
ApoI r'AATT_y
Cuts at: 531 1223 1936 2424 3478 3523 3667 4393 5036
Size: 692 713 488 1054 45 144 726 643
Cuts at: 5036 5313 6227 7319 8293 8585 9000 11349 11605
Size: 277 914 1092 974 292 415 2349 256
Cuts at: 11605 531
Size: 922
Fragments arranged by size:
2349 1092 1054 974 922 914 726 713
692 643 488 415 292 277 256 144
45
AseI AT'TA_AT
Cuts at: 20 20
Size: 11996
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 11996
AvaI C'yCGr_G
Cuts at: 198 216 2451 2930 4694 6015 7052 7135 9016
Size: 18 2235 479 1764 1321 1037 83 1881
Cuts at: 9016 198
Size: 3178
Fragments arranged by size:
3178 2235 1881 1764 1321 1037 479 83
18
AvrII C'CTAG_G
Cuts at: 5538 5538
Size: 11996
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5481 5514 5481
Size: 33 11963
BamHI G'GATC_C
Cuts at: 2829 6207 9021 2829
Size: 3378 2814 5804
Fragments arranged by size:
5804 3378 2814
BanI G'GyrC_C
Cuts at: 430 1004 1891 2210 4582 4746 8272 9012 9426
Size: 574 887 319 2372 164 3526 740 414
Cuts at: 9426 9674 9709 430
Size: 248 35 2717
Fragments arranged by size:
3526 2717 2372 887 740 574 414 319
248 164 35
BanII G_rGCy'C
Cuts at: 175 1582 2000 2964 2980 4655 6022 6418 7059
Size: 1407 418 964 16 1675 1367 396 641
Cuts at: 7059 7493 7631 8036 8813 9010 10040 175
Size: 434 138 405 777 197 1030 2131
Fragments arranged by size:
2131 1675 1407 1367 1030 964 777 641
434 418 405 396 197 138 16
BbeI G_GCGC'C
Cuts at: 434 9430 9678 434
Size: 8996 248 2752
Fragments arranged by size:
8996 2752 248
BbsI GAAGACnn'nnnn_
Cuts at: 143 746 3858 4372 6514 143
Size: 603 3112 514 2142 5625
Fragments arranged by size:
5625 3112 2142 603 514
BbvCI CC'TCA_GC
Cuts at: 482 482
Size: 11996
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 667 701 5913 5947 8815 8849 667
Size: 34 5212 34 2868 34 3814
Fragments arranged by size:
5212 3814 2868 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3447 7640 7713 9894 10655 11996 3447
Size: 4193 73 2181 761 1341 3447
Fragments arranged by size:
4193 3447 2181 1341 761 73
BclI T'GATC_A
Cuts at: 4107 5267 5621 6575 6926 9516 4107
Size: 1160 354 954 351 2590 6587
Fragments arranged by size:
6587 2590 1160 954 354 351
BglI GCCn_nnn'nGGC
Cuts at: 270 5845 6698 8624 8721 270
Size: 5575 853 1926 97 3545
Fragments arranged by size:
5575 3545 1926 853 97
BglII A'GATC_T
Cuts at: 813 2414 3069 5217 9511 11548 813
Size: 1601 655 2148 4294 2037 1261
Fragments arranged by size:
4294 2148 2037 1601 1261 655
BlpI GC'TnA_GC
Cuts at: 562 6101 7063 7370 7661 9108 562
Size: 5539 962 307 291 1447 3450
Fragments arranged by size:
5539 3450 1447 962 307 291
Bme1580I G_kGCm'C
Cuts at: 175 205 745 1112 1591 1896 2259 2651 2812
Size: 30 540 367 479 305 363 392 161
Cuts at: 2812 2964 4585 4751 6053 6418 6753 7059 8802
Size: 152 1621 166 1302 365 335 306 1743
Cuts at: 8802 8838 9621 9714 10770 175
Size: 36 783 93 1056 1401
Fragments arranged by size:
1743 1621 1401 1302 1056 783 540 479
392 367 365 363 335 306 305 166
161 152 93 36 30
BmgBI CAC'GTC
Cuts at: 1879 4629 6447 8763 1879
Size: 2750 1818 2316 5112
Fragments arranged by size:
5112 2750 2316 1818
BmrI ACTGGGnnnn_n'
Cuts at: 195 465 1890 3759 3987 6717 7670 9208 9289
Size: 270 1425 1869 228 2730 953 1538 81
Cuts at: 9289 9624 11768 195
Size: 335 2144 423
Fragments arranged by size:
2730 2144 1869 1538 1425 953 423 335
270 228 81
BmtI G_CTAG'C
Cuts at: 6254 6254
Size: 11996
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2994 4720 10363 2994
Size: 1726 5643 4627
Fragments arranged by size:
5643 4627 1726
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 215 280 418 439 678 742 830 1675 2482
Size: 65 138 21 239 64 88 845 807
Cuts at: 2482 3080 4136 4450 6460 7618 7797 8050 8110
Size: 598 1056 314 2010 1158 179 253 60
Cuts at: 8110 215
Size: 4101
Fragments arranged by size:
4101 2010 1158 1056 845 807 598 314
253 239 179 138 88 65 64 60
21
Bpu10I CC'TnA_GC
Cuts at: 482 1000 1999 3841 6338 482
Size: 518 999 1842 2497 6140
Fragments arranged by size:
6140 2497 1842 999 518
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 511 829 3291 3763 7495 9172 10051 10543 10841
Size: 318 2462 472 3732 1677 879 492 298
Cuts at: 10841 511
Size: 1666
Fragments arranged by size:
3732 2462 1677 1666 879 492 472 318
298
BsaI GGTCTCn'nnnn_
Cuts at: 201 1237 1278 1313 3346 4556 7373 8393 11700
Size: 1036 41 35 2033 1210 2817 1020 3307
Cuts at: 11700 201
Size: 497
Fragments arranged by size:
3307 2817 2033 1210 1036 1020 497 41
35
BsaAI yAC'GTr
Cuts at: 2813 3389 7971 8007 9349 2813
Size: 576 4582 36 1342 5460
Fragments arranged by size:
5460 4582 1342 576 36
BsaBI GATnn'nnATC
Cuts at: 6 2881 9510 11547 6
Size: 2875 6629 2037 455
Fragments arranged by size:
6629 2875 2037 455
BsaHI Gr'CG_yC
Cuts at: 221 431 1648 3753 4784 6566 9427 9675 10377
Size: 210 1217 2105 1031 1782 2861 248 702
Cuts at: 10377 11873 221
Size: 1496 344
Fragments arranged by size:
2861 2105 1782 1496 1217 1031 702 344
248 210
BsaWI w'CCGG_w
Cuts at: 1828 3926 6039 6055 9185 9706 10384 10658 10805
Size: 2098 2113 16 3130 521 678 274 147
Cuts at: 10805 11216 1828
Size: 411 2608
Fragments arranged by size:
3130 2608 2113 2098 678 521 411 274
147 16
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2630 2660 5204 5234 2630
Size: 30 2544 30 9392
Fragments arranged by size:
9392 2544 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 472 1660 2293 2296 2560 3721 4747 5212 6082
Size: 1188 633 3 264 1161 1026 465 870
Cuts at: 6082 7043 7294 7332 8200 8332 472
Size: 961 251 38 868 132 4136
Fragments arranged by size:
4136 1188 1161 1026 961 870 868 633
465 264 251 132 38 3
BseYI C'CCAG_C
Cuts at: 167 369 1117 4586 5171 5991 7058 7076 8148
Size: 202 748 3469 585 820 1067 18 1072
Cuts at: 8148 9421 10179 10756 11252 167
Size: 1273 758 577 496 911
Fragments arranged by size:
3469 1273 1072 1067 911 820 758 748
585 577 496 202 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 661 1357 2139 2371 3225 4922 5526 5873 7290
Size: 696 782 232 854 1697 604 347 1417
Cuts at: 7290 7525 7742 7864 661
Size: 235 217 122 4793
Fragments arranged by size:
4793 1697 1417 854 782 696 604 347
235 232 217 122
BsiEI CG_ry'CG
Cuts at: 104 8495 9538 9584 10792 104
Size: 8391 1043 46 1208 1308
Fragments arranged by size:
8391 1308 1208 1043 46
BsiHKAI G_wGCw'C
Cuts at: 728 745 1112 1582 1591 2000 2259 2774 2812
Size: 17 367 470 9 409 259 515 38
Cuts at: 2812 3052 3269 6446 6718 7376 7631 8036 8126
Size: 240 217 3177 272 658 255 405 90
Cuts at: 8126 8813 9010 9788 9978 10770 728
Size: 687 197 778 190 792 1954
Fragments arranged by size:
3177 1954 792 778 687 658 515 470
409 405 367 272 259 255 240 217
197 190 90 38 17 9
BsmI GAATG_Cn'
Cuts at: 1083 2467 1083
Size: 1384 10612
BsmBI CGTCTCn'nnnn_
Cuts at: 2472 2922 3741 5561 2472
Size: 450 819 1820 8907
Fragments arranged by size:
8907 1820 819 450
Bsp1286I G_dGCh'C
Cuts at: 175 205 728 745 1112 1582 1591 1896 2000
Size: 30 523 17 367 470 9 305 104
Cuts at: 2000 2259 2651 2774 2812 2964 2980 3052 3269
Size: 259 392 123 38 152 16 72 217
Cuts at: 3269 4585 4655 4751 6022 6053 6418 6446 6718
Size: 1316 70 96 1271 31 365 28 272
Cuts at: 6718 6753 7059 7376 7493 7631 8036 8126 8802
Size: 35 306 317 117 138 405 90 676
Cuts at: 8802 8813 8838 9010 9621 9714 9788 9978 10040
Size: 11 25 172 611 93 74 190 62
Cuts at: 10040 10770 175
Size: 730 1401
Fragments arranged by size:
1401 1316 1271 730 676 611 523 470
405 392 367 365 317 306 305 272
259 217 190 172 152 138 123 117
104 96 93 90 74 72 70 62
38 35 31 30 28 25 17 16
11 9
BspEI T'CCGG_A
Cuts at: 11216 11216
Size: 11996
BspHI T'CATG_A
Cuts at: 1574 5225 6138 7518 11172 1574
Size: 3651 913 1380 3654 2398
Fragments arranged by size:
3654 3651 2398 1380 913
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1044 1673 3044 4978 6249 6665 8873 9046 9260
Size: 629 1371 1934 1271 416 2208 173 214
Cuts at: 9260 9562 9943 1044
Size: 302 381 3097
Fragments arranged by size:
3097 2208 1934 1371 1271 629 416 381
302 214 173
BsrBI CCG'CTC
Cuts at: 50 366 10288 10342 11826 11991 50
Size: 316 9922 54 1484 165 55
Fragments arranged by size:
9922 1484 316 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1013 1128 5414 5652 6126 6770 8924 9908 1013
Size: 115 4286 238 474 644 2154 984 3101
Fragments arranged by size:
4286 3101 2154 984 644 474 238 115
BsrFI r'CCGG_y
Cuts at: 154 264 433 3607 4679 5765 6039 9994 10175
Size: 110 169 3174 1072 1086 274 3955 181
Cuts at: 10175 10384 154
Size: 209 1766
Fragments arranged by size:
3955 3174 1766 1086 1072 274 209 181
169 110
BsrGI T'GTAC_A
Cuts at: 2600 2600
Size: 11996
BssSI C'ACGA_G
Cuts at: 6844 10267 10625 6844
Size: 3423 358 8215
Fragments arranged by size:
8215 3423 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1024 2784 4552 5531 5651 8121 9373 1024
Size: 1760 1768 979 120 2470 1252 3647
Fragments arranged by size:
3647 2470 1768 1760 1252 979 120
BstBI TT'CG_AA
Cuts at: 4251 10357 4251
Size: 6106 5890
BstEII G'GTnAC_C
Cuts at: 5745 5745
Size: 11996
BstXI CCAn_nnnn'nTGG
Cuts at: 1020 4573 6641 7716 8163 8845 8940 1020
Size: 3553 2068 1075 447 682 95 4076
Fragments arranged by size:
4076 3553 2068 1075 682 447 95
BstYI r'GATC_y
Cuts at: 1 813 1426 2414 2829 3069 5217 5382 5517
Size: 812 613 988 415 240 2148 165 135
Cuts at: 5517 6207 6728 7217 8108 9021 9488 9511 9846
Size: 690 521 489 891 913 467 23 335
Cuts at: 9846 10092 11104 11190 11202 11548 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
2148 1012 988 913 891 812 690 613
521 489 467 449 415 346 335 246
240 165 135 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2308 3818 4846 5555 2308
Size: 1510 1028 709 8749
Fragments arranged by size:
8749 1510 1028 709
BtgI C'CryG_G
Cuts at: 107 191 248 375 1643 1765 2651 3077 4403
Size: 84 57 127 1268 122 886 426 1326
Cuts at: 4403 4727 5562 6419 6719 8156 8879 107
Size: 324 835 857 300 1437 723 3224
Fragments arranged by size:
3224 1437 1326 1268 886 857 835 723
426 324 300 127 122 84 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 319 1220 5669 8685 9946 10127 10290 319
Size: 901 4449 3016 1261 181 163 2025
Fragments arranged by size:
4449 3016 2025 1261 901 181 163
BtsI GCAGTG_nn'
Cuts at: 138 726 957 1025 2259 3258 3677 4614 4923
Size: 588 231 68 1234 999 419 937 309
Cuts at: 4923 5509 5607 5904 6578 6789 7775 8358 9239
Size: 586 98 297 674 211 986 583 881
Cuts at: 9239 138
Size: 2895
Fragments arranged by size:
2895 1234 999 986 937 881 674 588
586 583 419 309 297 231 211 98
68
ClaI AT'CG_AT
Cuts at: 5487 5487
Size: 11996
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 4481 4516 4481
Size: 35 11961
DraI TTT'AAA
Cuts at: 977 8995 977
Size: 8018 3978
DraIII CAC_nnn'GTG
Cuts at: 3264 7765 8468 3264
Size: 4501 703 6792
Fragments arranged by size:
6792 4501 703
DrdI GACnn_nn'nnGTC
Cuts at: 2914 9702 10560 2914
Size: 6788 858 4350
Fragments arranged by size:
6788 4350 858
EaeI y'GGCC_r
Cuts at: 266 2870 5706 5846 6528 7426 8492 8625 8679
Size: 2604 2836 140 682 898 1066 133 54
Cuts at: 8679 8942 9581 9755 10146 10173 11518 266
Size: 263 639 174 391 27 1345 744
Fragments arranged by size:
2836 2604 1345 1066 898 744 682 639
391 263 174 140 133 54 27
EagI C'GGCC_G
Cuts at: 8492 9581 8492
Size: 1089 10907
EarI CTCTTCn'nnn_
Cuts at: 356 377 1538 1706 2746 2992 3040 4208 4369
Size: 21 1161 168 1040 246 48 1168 161
Cuts at: 4369 4502 4643 6361 6982 7409 7603 7933 8818
Size: 133 141 1718 621 427 194 330 885
Cuts at: 8818 10019 10229 356
Size: 1201 210 2123
Fragments arranged by size:
2123 1718 1201 1168 1161 1040 885 621
427 330 246 210 194 168 161 141
133 48 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 10204 10514 10660 11806 10204
Size: 310 146 1146 10394
Fragments arranged by size:
10394 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1580 1998 7629 8034 8811 9008 1580
Size: 418 5631 405 777 197 4568
Fragments arranged by size:
5631 4568 777 418 405 197
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 215 280 418 439 535 678 742 784 830
Size: 65 138 21 96 143 64 42 46
Cuts at: 830 1075 1675 1687 1913 1975 2302 2482 3064
Size: 245 600 12 226 62 327 180 582
Cuts at: 3064 3080 3322 3852 4136 4450 4559 4667 4798
Size: 16 242 530 284 314 109 108 131
Cuts at: 4798 4903 6299 6460 6838 7219 7270 7618 7797
Size: 105 1396 161 378 381 51 348 179
Cuts at: 7797 8050 8110 8364 9821 10253 11000 11268 215
Size: 253 60 254 1457 432 747 268 943
Fragments arranged by size:
1457 1396 943 747 600 582 530 432
381 378 348 327 314 284 268 254
253 245 242 226 180 179 161 143
138 131 109 108 105 96 65 64
62 60 51 46 42 21 16 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2047 4556 7882 8755 2047
Size: 2509 3326 873 5288
Fragments arranged by size:
5288 3326 2509 873
EcoO109I rG'GnC_Cy
Cuts at: 171 522 639 2312 2960 2961 4071 5111 6033
Size: 351 117 1673 648 1 1110 1040 922
Cuts at: 6033 6415 6867 7158 7311 7479 9135 171
Size: 382 452 291 153 168 1656 3032
Fragments arranged by size:
3032 1673 1656 1110 1040 922 648 452
382 351 291 168 153 117 1
EcoRI G'AATT_C
Cuts at: 531 1223 3478 5313 9000 531
Size: 692 2255 1835 3687 3527
Fragments arranged by size:
3687 3527 2255 1835 692
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5311 10015 5311
Size: 4704 7292
FspI TGC'GCA
Cuts at: 9777 9777
Size: 11996
HaeII r_GCGC'y
Cuts at: 238 256 434 1393 2181 3778 5840 7709 7857
Size: 18 178 959 788 1597 2062 1869 148
Cuts at: 7857 7871 9430 9678 10700 11679 238
Size: 14 1559 248 1022 979 555
Fragments arranged by size:
2062 1869 1597 1559 1022 979 959 788
555 248 178 148 18 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 637 669 1612 1644 2626 2658 4642 4674 5940
Size: 32 943 32 982 32 1984 32 1266
Cuts at: 5940 5972 5998 6030 8613 8645 9838 9868 9870
Size: 32 26 32 2583 32 1193 30 2
Cuts at: 9870 9900 637
Size: 30 2733
Fragments arranged by size:
2733 2583 1984 1266 1193 982 943 32
32 32 32 32 32 32 30 30
26 2
HincII GTy'rAC
Cuts at: 1697 3214 4185 5586 9035 1697
Size: 1517 971 1401 3449 4658
Fragments arranged by size:
4658 3449 1517 1401 971
HindIII A'AGCT_T
Cuts at: 1987 1987
Size: 11996
KasI G'GCGC_C
Cuts at: 430 9426 9674 430
Size: 8996 248 2752
Fragments arranged by size:
8996 2752 248
KpnI G_GTAC'C
Cuts at: 2214 9016 2214
Size: 6802 5194
MluI A'CGCG_T
Cuts at: 11429 11429
Size: 11996
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 497 911 3523 3872 4418 4937 8474 10667 10851
Size: 414 2612 349 546 519 3537 2193 184
Cuts at: 10851 497
Size: 1642
Fragments arranged by size:
3537 2612 2193 1642 546 519 414 349
184
MscI TGG'CCA
Cuts at: 2872 5848 8627 8944 9757 2872
Size: 2976 2779 317 813 5111
Fragments arranged by size:
5111 2976 2779 813 317
MslI CAynn'nnrTG
Cuts at: 131 1018 2610 2612 2749 3413 4823 5230 5261
Size: 887 1592 2 137 664 1410 407 31
Cuts at: 5261 6494 9985 10112 131
Size: 1233 3491 127 2015
Fragments arranged by size:
3491 2015 1592 1410 1233 887 664 407
137 127 31 2
MspA1I CmG'CkG
Cuts at: 151 193 250 377 783 3042 4645 5495 6804
Size: 42 57 127 406 2259 1603 850 1309
Cuts at: 6804 6816 6887 7014 7062 7665 7951 8082 8384
Size: 12 71 127 48 603 286 131 302
Cuts at: 8384 8900 9107 9421 9781 10794 11039 151
Size: 516 207 314 360 1013 245 1108
Fragments arranged by size:
2259 1603 1309 1108 1013 850 603 516
406 360 314 302 286 245 207 131
127 127 71 57 48 42 12
NaeI GCC'GGC
Cuts at: 266 435 4681 10177 266
Size: 169 4246 5496 2085
Fragments arranged by size:
5496 4246 2085 169
NarI GG'CG_CC
Cuts at: 431 9427 9675 431
Size: 8996 248 2752
Fragments arranged by size:
8996 2752 248
NcoI C'CATG_G
Cuts at: 107 1643 2651 4727 6719 8156 107
Size: 1536 1008 2076 1992 1437 3947
Fragments arranged by size:
3947 2076 1992 1536 1437 1008
NdeI CA'TA_TG
Cuts at: 8051 8051
Size: 11996
NgoMIV G'CCGG_C
Cuts at: 264 433 4679 10175 264
Size: 169 4246 5496 2085
Fragments arranged by size:
5496 4246 2085 169
NheI G'CTAG_C
Cuts at: 6250 6250
Size: 11996
NspI r_CATG'y
Cuts at: 516 741 1047 1404 1712 4061 5349 5412 5928
Size: 225 306 357 308 2349 1288 63 516
Cuts at: 5928 8148 8174 9049 9549 10456 516
Size: 2220 26 875 500 907 2056
Fragments arranged by size:
2349 2220 2056 1288 907 875 516 500
357 308 306 225 63 26
PasI CC'CwG_GG
Cuts at: 2965 3454 6516 2965
Size: 489 3062 8445
Fragments arranged by size:
8445 3062 489
PciI A'CATG_T
Cuts at: 512 737 5408 10452 512
Size: 225 4671 5044 2056
Fragments arranged by size:
5044 4671 2056 225
PflMI CCAn_nnn'nTGG
Cuts at: 2346 3111 3771 5580 5742 8181 2346
Size: 765 660 1809 162 2439 6161
Fragments arranged by size:
6161 2439 1809 765 660 162
PfoI T'CCnGG_A
Cuts at: 407 4871 7307 7595 7916 8027 8418 407
Size: 4464 2436 288 321 111 391 3985
Fragments arranged by size:
4464 3985 2436 391 321 288 111
PmeI GTTT'AAAC
Cuts at: 8995 8995
Size: 11996
PmlI CAC'GTG
Cuts at: 7971 8007 7971
Size: 36 11960
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 11135 11167 11135
Size: 32 11964
PpuMI rG'GwC_Cy
Cuts at: 2312 5111 6867 7158 7311 2312
Size: 2799 1756 291 153 6997
Fragments arranged by size:
6997 2799 1756 291 153
PshAI GACnn'nnGTC
Cuts at: 3644 4568 3644
Size: 924 11072
PspOMI G'GGCC_C
Cuts at: 171 2960 6414 7055 171
Size: 2789 3454 641 5112
Fragments arranged by size:
5112 3454 2789 641
PspXI vC'TCGA_Gb
Cuts at: 6015 6015
Size: 11996
PstI C_TGCA'G
Cuts at: 291 2266 2492 2522 2789 3041 4626 5504 6246
Size: 1975 226 30 267 252 1585 878 742
Cuts at: 6246 6615 7571 7835 8117 8858 8921 9043 9728
Size: 369 956 264 282 741 63 122 685
Cuts at: 9728 291
Size: 2559
Fragments arranged by size:
2559 1975 1585 956 878 742 741 685
369 282 267 264 252 226 122 63
30
PvuI CG_AT'CG
Cuts at: 104 104
Size: 11996
PvuII CAG'CTG
Cuts at: 151 783 3042 4645 6804 6816 7014 7062 7951
Size: 632 2259 1603 2159 12 198 48 889
Cuts at: 7951 8082 9421 9781 151
Size: 131 1339 360 2366
Fragments arranged by size:
2366 2259 2159 1603 1339 889 632 360
198 131 48 12
RsrII CG'GwC_CG
Cuts at: 10191 10191
Size: 11996
SacI G_AGCT'C
Cuts at: 1582 2000 7631 8036 8813 9010 1582
Size: 418 5631 405 777 197 4568
Fragments arranged by size:
5631 4568 777 418 405 197
SacII CC_GC'GG
Cuts at: 194 251 378 194
Size: 57 127 11812
Fragments arranged by size:
11812 127 57
SalI G'TCGA_C
Cuts at: 9033 9033
Size: 11996
SapI GCTCTTCn'nnn_
Cuts at: 356 2992 3040 4502 4643 8818 10019 10229 356
Size: 2636 48 1462 141 4175 1201 210 2123
Fragments arranged by size:
4175 2636 2123 1462 1201 210 141 48
SbfI CC_TGCA'GG
Cuts at: 9043 9043
Size: 11996
ScaI AGT'ACT
Cuts at: 7072 11513 7072
Size: 4441 7555
SexAI A'CCwGG_T
Cuts at: 2345 2345
Size: 11996
SfcI C'TryA_G
Cuts at: 33 287 583 1067 1430 2262 2488 2518 2785
Size: 254 296 484 363 832 226 30 267
Cuts at: 2785 3037 3312 4622 5500 5832 6155 6242 6260
Size: 252 275 1310 878 332 323 87 18
Cuts at: 6260 6611 7567 7831 8113 8475 8854 8917 9039
Size: 351 956 264 282 362 379 63 122
Cuts at: 9039 9724 10717 10908 33
Size: 685 993 191 1121
Fragments arranged by size:
1310 1121 993 956 878 832 685 484
379 363 362 351 332 323 296 282
275 267 264 254 252 226 191 122
87 63 30 18
SfoI GGC'GCC
Cuts at: 432 9428 9676 432
Size: 8996 248 2752
Fragments arranged by size:
8996 2752 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 11996
SmaI CCC'GGG
Cuts at: 218 9018 218
Size: 8800 3196
SmlI C'TyrA_G
Cuts at: 526 844 2930 3270 3778 6015 7474 9151 10066
Size: 318 2086 340 508 2237 1459 1677 915
Cuts at: 10066 10558 10820 526
Size: 492 262 1702
Fragments arranged by size:
2237 2086 1702 1677 1459 915 508 492
340 318 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 11996
SphI G_CATG'C
Cuts at: 5928 8174 9049 9549 5928
Size: 2246 875 500 8375
Fragments arranged by size:
8375 2246 875 500
SrfI GCCC'GGGC
Cuts at: 218 218
Size: 11996
SspI AAT'ATT
Cuts at: 2134 2134
Size: 11996
StuI AGG'CCT
Cuts at: 463 589 5356 5972 463
Size: 126 4767 616 6487
Fragments arranged by size:
6487 4767 616 126
StyI C'CwwG_G
Cuts at: 107 1643 2043 2591 2651 3009 4235 4727 4911
Size: 1536 400 548 60 358 1226 492 184
Cuts at: 4911 5538 6719 8156 9130 9484 107
Size: 627 1181 1437 974 354 2619
Fragments arranged by size:
2619 1536 1437 1226 1181 974 627 548
492 400 358 354 184 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3935 4600 4686 5450 5753 5983 6667 7255 7421
Size: 665 86 764 303 230 684 588 166
Cuts at: 7421 7597 8384 8593 9579 9712 10171 10380 3935
Size: 176 787 209 986 133 459 209 5551
Fragments arranged by size:
5551 986 787 764 684 665 588 459
303 230 209 209 176 166 133 86
TatI w'GTAC_w
Cuts at: 2529 2600 3152 7070 7337 11511 2529
Size: 71 552 3918 267 4174 3014
Fragments arranged by size:
4174 3918 3014 552 267 71
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2630 2662 2764 2796 6697 6729 8186 8218 11513
Size: 32 102 32 3901 32 1457 32 3295
Cuts at: 11513 11545 2630
Size: 32 3081
Fragments arranged by size:
3901 3295 3081 1457 102 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 9793 9793
Size: 11996
XbaI T'CTAG_A
Cuts at: 9027 9027
Size: 11996
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 182 2136 9491 182
Size: 1954 7355 2687
Fragments arranged by size:
7355 2687 1954
XhoI C'TCGA_G
Cuts at: 2930 6015 2930
Size: 3085 8911
XmaI C'CCGG_G
Cuts at: 216 9016 216
Size: 8800 3196
ZraI GAC'GTC
Cuts at: 4785 4785
Size: 11996
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AloI AscI BfrBI BsiWI BssHII BstZ17I
EcoRV FseI FspAI HpaI MfeI NotI NruI NsiI
PacI PsiI PsrI SanDI SfiI SgrAI SnaBI SwaI
XmnI