Restriction map for
pF1KA0400
(Circular) (Six-base) MAPSORT of: pF1KA0400.seq Check: 285 from: 1 to: 6131
pF1KA0400 6131 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AatII G_ACGT'C
Cuts at: 1412 1412
Size: 6131
AccI GT'mk_AC
Cuts at: 853 1919 3169 853
Size: 1066 1250 3815
Fragments arranged by size:
3815 1250 1066
Acc65I G'GTAC_C
Cuts at: 1126 3147 1126
Size: 2021 4110
AclI AA'CG_TT
Cuts at: 5971 5971
Size: 6131
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 424 520 736 742 1265 2284 3956 4388 4741
Size: 96 216 6 523 1019 1672 432 353
Cuts at: 4741 5403 424
Size: 662 1152
Fragments arranged by size:
1672 1152 1019 662 523 432 353 216
96 6
AfeI AGC'GCT
Cuts at: 2960 2960
Size: 6131
AflIII A'CryG_T
Cuts at: 3483 5283 5564 3483
Size: 1800 281 4050
Fragments arranged by size:
4050 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1996 2918 1996
Size: 922 5209
AlwNI CAG_nnn'CTG
Cuts at: 342 765 1533 3436 4874 342
Size: 423 768 1903 1438 1599
Fragments arranged by size:
1903 1599 1438 768 423
ApaLI G'TGCA_C
Cuts at: 3109 4969 3109
Size: 1860 4271
ApoI r'AATT_y
Cuts at: 133 965 3135 5484 5740 133
Size: 832 2170 2349 256 524
Fragments arranged by size:
2349 2170 832 524 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6131
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6131
AvaI C'yCGr_G
Cuts at: 3151 3151
Size: 6131
AvrII C'CTAG_G
Cuts at: 2914 2914
Size: 6131
BamHI G'GATC_C
Cuts at: 1061 3156 1061
Size: 2095 4036
BanI G'GyrC_C
Cuts at: 165 1126 1428 3147 3561 3809 3844 165
Size: 961 302 1719 414 248 35 2452
Fragments arranged by size:
2452 1719 961 414 302 248 35
BanII G_rGCy'C
Cuts at: 2290 2403 2996 3145 4175 2290
Size: 113 593 149 1030 4246
Fragments arranged by size:
4246 1030 593 149 113
BbeI G_GCGC'C
Cuts at: 169 1432 3565 3813 169
Size: 1263 2133 248 2487
Fragments arranged by size:
2487 2133 1263 248
BbsI GAAGACnn'nnnn_
Cuts at: 1183 2201 2264 2530 1183
Size: 1018 63 266 4784
Fragments arranged by size:
4784 1018 266 63
BbvCI CC'TCA_GC
Cuts at: 1628 1628
Size: 6131
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1648 1682 90
Size: 34 1524 34 4539
Fragments arranged by size:
4539 1524 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4029 5085 6131 4029
Size: 1056 1046 4029
Fragments arranged by size:
4029 1056 1046
BclI T'GATC_A
Cuts at: 767 3017 3651 767
Size: 2250 634 3247
Fragments arranged by size:
3247 2250 634
BfrBI ATG'CAT
Cuts at: 1315 1315
Size: 6131
BglII A'GATC_T
Cuts at: 119 3646 5683 119
Size: 3527 2037 567
Fragments arranged by size:
3527 2037 567
BlpI GC'TnA_GC
Cuts at: 3243 3243
Size: 6131
Bme1580I G_kGCm'C
Cuts at: 3113 3756 3849 4973 3113
Size: 643 93 1124 4271
Fragments arranged by size:
4271 1124 643 93
BmgBI CAC'GTC
Cuts at: 243 243
Size: 6131
BmrI ACTGGGnnnn_n'
Cuts at: 1558 2234 3343 3424 3759 5903 1558
Size: 676 1109 81 335 2144 1786
Fragments arranged by size:
2144 1786 1109 676 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1395 4498 1395
Size: 3103 3028
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 162 364 1358 162
Size: 202 994 4935
Fragments arranged by size:
4935 994 202
Bpu10I CC'TnA_GC
Cuts at: 1628 2945 1628
Size: 1317 4814
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2002 2083 2706 3307 4186 4900 5198 2002
Size: 81 623 601 879 714 298 2935
Fragments arranged by size:
2935 879 714 623 601 298 81
BsaI GGTCTCn'nnnn_
Cuts at: 139 5835 139
Size: 5696 435
BsaAI yAC'GTr
Cuts at: 312 3484 312
Size: 3172 2959
BsaBI GATnn'nnATC
Cuts at: 6 3071 3645 5682 6
Size: 3065 574 2037 455
Fragments arranged by size:
3065 2037 574 455
BsaHI Gr'CG_yC
Cuts at: 166 1409 1429 2665 3562 3810 4512 6008 166
Size: 1243 20 1236 897 248 702 1496 289
Fragments arranged by size:
1496 1243 1236 897 702 289 248 20
BsaWI w'CCGG_w
Cuts at: 248 1486 3320 3841 4519 4930 5077 5351 248
Size: 1238 1834 521 678 411 147 274 1028
Fragments arranged by size:
1834 1238 1028 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 106 136 460 490 1183 1213 106
Size: 30 324 30 693 30 5024
Fragments arranged by size:
5024 693 324 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 244 1204 1719 2131 2493 3049 244
Size: 960 515 412 362 556 3326
Fragments arranged by size:
3326 960 556 515 412 362
BseYI C'CCAG_C
Cuts at: 1503 2283 2371 2429 2443 2668 2758 3556 4314
Size: 780 88 58 14 225 90 798 758
Cuts at: 4314 4979 5387 1503
Size: 665 408 2247
Fragments arranged by size:
2247 798 780 758 665 408 225 90
88 58 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1408 1966 2888 1408
Size: 558 922 4651
Fragments arranged by size:
4651 922 558
BsiEI CG_ry'CG
Cuts at: 104 3673 3719 4949 104
Size: 3569 46 1230 1286
Fragments arranged by size:
3569 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 979 2111 2996 3113 3145 3923 4113 4973 979
Size: 1132 885 117 32 778 190 860 2137
Fragments arranged by size:
2137 1132 885 860 778 190 117 32
BsmI GAATG_Cn'
Cuts at: 1593 1671 3091 1593
Size: 78 1420 4633
Fragments arranged by size:
4633 1420 78
Bsp1286I G_dGCh'C
Cuts at: 979 2111 2290 2403 2996 3113 3145 3756 3849
Size: 1132 179 113 593 117 32 611 93
Cuts at: 3849 3923 4113 4175 4973 979
Size: 74 190 62 798 2137
Fragments arranged by size:
2137 1132 798 611 593 190 179 117
113 93 74 62 32
BspEI T'CCGG_A
Cuts at: 1486 1486
Size: 6131
BspHI T'CATG_A
Cuts at: 4563 4563
Size: 6131
BspMI ACCTGCnnnn'nnnn_
Cuts at: 930 1166 1174 1480 3181 3395 3697 4078 930
Size: 236 8 306 1701 214 302 381 2983
Fragments arranged by size:
2983 1701 381 306 302 236 214 8
BsrBI CCG'CTC
Cuts at: 50 649 1029 4423 4477 5961 6126 50
Size: 599 380 3394 54 1484 165 55
Fragments arranged by size:
3394 1484 599 380 165 55 54
BsrDI GCAATG_nn'
Cuts at: 934 1410 2084 4043 934
Size: 476 674 1959 3022
Fragments arranged by size:
3022 1959 674 476
BsrFI r'CCGG_y
Cuts at: 1136 2249 2261 2555 4129 4310 1136
Size: 1113 12 294 1574 181 2957
Fragments arranged by size:
2957 1574 1113 294 181 12
BssSI C'ACGA_G
Cuts at: 2104 4402 5110 2104
Size: 2298 708 3125
Fragments arranged by size:
3125 2298 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2341 3508 2341
Size: 1167 4964
BstBI TT'CG_AA
Cuts at: 4492 4492
Size: 6131
BstXI CCAn_nnnn'nTGG
Cuts at: 1458 2676 2886 3067 1458
Size: 1218 210 181 4522
Fragments arranged by size:
4522 1218 210 181
BstYI r'GATC_y
Cuts at: 1 119 528 858 1061 1434 1818 1888 2574
Size: 118 409 330 203 373 384 70 686
Cuts at: 2574 2717 2844 3072 3156 3623 3646 3981 4227
Size: 143 127 228 84 467 23 335 246
Cuts at: 4227 4533 4545 4631 5683 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 686 467 449 409 384 373 335
330 306 246 228 203 143 127 118
86 84 70 23 12
Bsu36I CC'TnA_GG
Cuts at: 1001 1187 1001
Size: 186 5945
BtgI C'CryG_G
Cuts at: 170 618 818 1122 1892 2473 170
Size: 448 200 304 770 581 3828
Fragments arranged by size:
3828 770 581 448 304 200
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4081 4262 4425 4081
Size: 181 163 5787
Fragments arranged by size:
5787 181 163
BtsI GCAGTG_nn'
Cuts at: 208 3374 208
Size: 3166 2965
DraI TTT'AAA
Cuts at: 1307 3130 1307
Size: 1823 4308
DraIII CAC_nnn'GTG
Cuts at: 2519 2519
Size: 6131
DrdI GACnn_nn'nnGTC
Cuts at: 3837 5181 3837
Size: 1344 4787
EaeI y'GGCC_r
Cuts at: 140 221 1841 2321 3057 3716 3890 4281 4308
Size: 81 1620 480 736 659 174 391 27
Cuts at: 4308 5653 140
Size: 1345 618
Fragments arranged by size:
1620 1345 736 659 618 480 391 174
81 27
EagI C'GGCC_G
Cuts at: 3716 3716
Size: 6131
EarI CTCTTCn'nnn_
Cuts at: 317 656 1045 1913 2612 4154 4364 317
Size: 339 389 868 699 1542 210 2084
Fragments arranged by size:
2084 1542 868 699 389 339 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2567 4339 5081 5227 5941 2567
Size: 1772 742 146 714 2757
Fragments arranged by size:
2757 1772 742 714 146
EcoICRI GAG'CTC
Cuts at: 2994 3143 2994
Size: 149 5982
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 162 364 424 520 736 742 1265 1358 2284
Size: 202 60 96 216 6 523 93 926
Cuts at: 2284 3956 4388 4741 5403 162
Size: 1672 432 353 662 890
Fragments arranged by size:
1672 926 890 662 523 432 353 216
202 96 93 60 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2344 2344
Size: 6131
EcoO109I rG'GnC_Cy
Cuts at: 507 2732 2836 2869 3270 507
Size: 2225 104 33 401 3368
Fragments arranged by size:
3368 2225 401 104 33
EcoRI G'AATT_C
Cuts at: 133 3135 133
Size: 3002 3129
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1218 4150 1218
Size: 2932 3199
FspI TGC'GCA
Cuts at: 3912 3912
Size: 6131
HaeII r_GCGC'y
Cuts at: 169 684 1432 2097 2459 2962 3565 3813 5043
Size: 515 748 665 362 503 603 248 1230
Cuts at: 5043 5814 169
Size: 771 486
Fragments arranged by size:
1230 771 748 665 603 515 503 486
362 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 106 138 1888 1920 3973 4003 4005 4035 106
Size: 32 1750 32 2053 30 2 30 2202
Fragments arranged by size:
2202 2053 1750 32 32 30 30 2
HincII GTy'rAC
Cuts at: 431 723 3170 431
Size: 292 2447 3392
Fragments arranged by size:
3392 2447 292
HindIII A'AGCT_T
Cuts at: 551 1301 1744 1799 551
Size: 750 443 55 4883
Fragments arranged by size:
4883 750 443 55
KasI G'GCGC_C
Cuts at: 165 1428 3561 3809 165
Size: 1263 2133 248 2487
Fragments arranged by size:
2487 2133 1263 248
KpnI G_GTAC'C
Cuts at: 1130 3151 1130
Size: 2021 4110
MluI A'CGCG_T
Cuts at: 5564 5564
Size: 6131
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 110 2315 4890 5074 110
Size: 2205 2575 184 1167
Fragments arranged by size:
2575 2205 1167 184
MscI TGG'CCA
Cuts at: 1843 2323 3059 3892 1843
Size: 480 736 833 4082
Fragments arranged by size:
4082 833 736 480
MslI CAynn'nnrTG
Cuts at: 3065 4120 4247 3065
Size: 1055 127 4949
Fragments arranged by size:
4949 1055 127
MspA1I CmG'CkG
Cuts at: 1627 2079 2283 2584 2598 3242 3556 3916 4700
Size: 452 204 301 14 644 314 360 784
Cuts at: 4700 4945 1627
Size: 245 2813
Fragments arranged by size:
2813 784 644 452 360 314 301 245
204 14
NaeI GCC'GGC
Cuts at: 2557 4312 2557
Size: 1755 4376
NarI GG'CG_CC
Cuts at: 166 1429 3562 3810 166
Size: 1263 2133 248 2487
Fragments arranged by size:
2487 2133 1263 248
NcoI C'CATG_G
Cuts at: 618 1122 618
Size: 504 5627
NgoMIV G'CCGG_C
Cuts at: 2555 4310 2555
Size: 1755 4376
NsiI A_TGCA'T
Cuts at: 1317 1317
Size: 6131
NspI r_CATG'y
Cuts at: 3184 3684 5287 3184
Size: 500 1603 4028
Fragments arranged by size:
4028 1603 500
PciI A'CATG_T
Cuts at: 5283 5283
Size: 6131
PflMI CCAn_nnn'nTGG
Cuts at: 342 342
Size: 6131
PfoI T'CCnGG_A
Cuts at: 1519 1519
Size: 6131
PmeI GTTT'AAAC
Cuts at: 3130 3130
Size: 6131
PmlI CAC'GTG
Cuts at: 312 312
Size: 6131
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4577 4609 4577
Size: 32 6099
PpuMI rG'GwC_Cy
Cuts at: 507 2836 2869 507
Size: 2329 33 3769
Fragments arranged by size:
3769 2329 33
PstI C_TGCA'G
Cuts at: 2439 2897 3178 3863 2439
Size: 458 281 685 4707
Fragments arranged by size:
4707 685 458 281
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6131
PvuII CAG'CTG
Cuts at: 1627 2283 2584 3556 3916 1627
Size: 656 301 972 360 3842
Fragments arranged by size:
3842 972 656 360 301
RsrII CG'GwC_CG
Cuts at: 4326 4326
Size: 6131
SacI G_AGCT'C
Cuts at: 2996 3145 2996
Size: 149 5982
SalI G'TCGA_C
Cuts at: 3168 3168
Size: 6131
SapI GCTCTTCn'nnn_
Cuts at: 317 4154 4364 317
Size: 3837 210 2084
Fragments arranged by size:
3837 2084 210
SbfI CC_TGCA'GG
Cuts at: 3178 3178
Size: 6131
ScaI AGT'ACT
Cuts at: 5648 5648
Size: 6131
SfcI C'TryA_G
Cuts at: 33 694 2435 2893 3174 3859 4827 5018 33
Size: 661 1741 458 281 685 968 191 1146
Fragments arranged by size:
1741 1146 968 685 661 458 281 191
SfoI GGC'GCC
Cuts at: 167 1430 3563 3811 167
Size: 1263 2133 248 2487
Fragments arranged by size:
2487 2133 1263 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6131
SmaI CCC'GGG
Cuts at: 3153 3153
Size: 6131
SmlI C'TyrA_G
Cuts at: 2017 2098 2685 3286 4201 4915 5177 2017
Size: 81 587 601 915 714 262 2971
Fragments arranged by size:
2971 915 714 601 587 262 81
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6131
SphI G_CATG'C
Cuts at: 3184 3684 3184
Size: 500 5631
SspI AAT'ATT
Cuts at: 1581 1581
Size: 6131
StuI AGG'CCT
Cuts at: 2041 2041
Size: 6131
StyI C'CwwG_G
Cuts at: 618 1122 2345 2914 3265 3619 618
Size: 504 1223 569 351 354 3130
Fragments arranged by size:
3130 1223 569 504 354 351
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 349 1039 2014 2922 3714 3847 4306 4515 349
Size: 690 975 908 792 133 459 209 1965
Fragments arranged by size:
1965 975 908 792 690 459 209 133
TatI w'GTAC_w
Cuts at: 329 1545 1694 5646 329
Size: 1216 149 3952 814
Fragments arranged by size:
3952 1216 814 149
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1183 1215 3031 3063 5648 5680 1183
Size: 32 1816 32 2585 32 1634
Fragments arranged by size:
2585 1816 1634 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1636 3928 1636
Size: 2292 3839
XbaI T'CTAG_A
Cuts at: 3162 3162
Size: 6131
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1439 2137 3626 1439
Size: 698 1489 3944
Fragments arranged by size:
3944 1489 698
XmaI C'CCGG_G
Cuts at: 3151 3151
Size: 6131
ZraI GAC'GTC
Cuts at: 1410 1410
Size: 6131
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AflII AgeI AhdI AleI AloI ApaI AscI
BaeI BglI BmtI BsiWI BsmBI BsrGI BssHII BstEII
BstZ17I ClaI CspCI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI PacI PasI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SwaI XhoI XmnI