Restriction map for
pF1KA0404
(Circular) (Six-base) MAPSORT of: pF1KA0404.seq Check: 584 from: 1 to: 8927
pF1KA0404 8927 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 974 2206 4341 974
Size: 1232 2135 5560
Fragments arranged by size:
5560 2135 1232
AatII G_ACGT'C
Cuts at: 1432 1432
Size: 8927
AccI GT'mk_AC
Cuts at: 1268 2594 4046 5965 1268
Size: 1326 1452 1919 4230
Fragments arranged by size:
4230 1919 1452 1326
Acc65I G'GTAC_C
Cuts at: 3001 5943 3001
Size: 2942 5985
AclI AA'CG_TT
Cuts at: 8767 8767
Size: 8927
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 982 1408 2926 2994 3992 5082 5248 6752 7184
Size: 426 1518 68 998 1090 166 1504 432
Cuts at: 7184 7537 8199 982
Size: 353 662 1710
Fragments arranged by size:
1710 1518 1504 1090 998 662 432 426
353 166 68
AfeI AGC'GCT
Cuts at: 4793 5605 4793
Size: 812 8115
AflIII A'CryG_T
Cuts at: 3239 3762 6279 8079 8360 3239
Size: 523 2517 1800 281 3806
Fragments arranged by size:
3806 2517 1800 523 281
AgeI A'CCGG_T
Cuts at: 5513 5513
Size: 8927
AhdI GACnn_n'nnGTC
Cuts at: 1619 5562 1619
Size: 3943 4984
AleI CACnn'nnGTG
Cuts at: 631 1588 3253 3773 4360 4742 631
Size: 957 1665 520 587 382 4816
Fragments arranged by size:
4816 1665 957 587 520 382
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1606 1666 3791 4336 1606
Size: 60 2125 545 6197
Fragments arranged by size:
6197 2125 545 60
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2320 2352 2320
Size: 32 8895
AlwNI CAG_nnn'CTG
Cuts at: 451 555 699 1005 1107 1119 1138 1797 4618
Size: 104 144 306 102 12 19 659 2821
Cuts at: 4618 4638 4683 5491 6232 7670 451
Size: 20 45 808 741 1438 1708
Fragments arranged by size:
2821 1708 1438 808 741 659 306 144
104 102 45 20 19 12
ApaI G_GGCC'C
Cuts at: 1505 1905 4077 4105 5356 1505
Size: 400 2172 28 1251 5076
Fragments arranged by size:
5076 2172 1251 400 28
ApaLI G'TGCA_C
Cuts at: 1651 2968 3766 7765 1651
Size: 1317 798 3999 2813
Fragments arranged by size:
3999 2813 1317 798
ApoI r'AATT_y
Cuts at: 5931 8280 8536 5931
Size: 2349 256 6322
Fragments arranged by size:
6322 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 8927
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8927
AvaI C'yCGr_G
Cuts at: 978 1514 1691 2024 2685 3207 3349 4229 4358
Size: 536 177 333 661 522 142 880 129
Cuts at: 4358 4972 4979 5842 5947 978
Size: 614 7 863 105 3958
Fragments arranged by size:
3958 880 863 661 614 536 522 333
177 142 129 105 7
BamHI G'GATC_C
Cuts at: 5952 5952
Size: 8927
BanI G'GyrC_C
Cuts at: 738 1075 1696 3001 4329 4391 5758 5943 6357
Size: 337 621 1305 1328 62 1367 185 414
Cuts at: 6357 6605 6640 738
Size: 248 35 3025
Fragments arranged by size:
3025 1367 1328 1305 621 414 337 248
185 62 35
BanII G_rGCy'C
Cuts at: 343 536 824 851 958 1145 1253 1505 1750
Size: 193 288 27 107 187 108 252 245
Cuts at: 1750 1905 1979 2135 2429 4077 4105 4468 4986
Size: 155 74 156 294 1648 28 363 518
Cuts at: 4986 5261 5356 5941 6971 343
Size: 275 95 585 1030 2299
Fragments arranged by size:
2299 1648 1030 585 518 363 294 288
275 252 245 193 187 156 155 108
107 95 74 28 27
BbeI G_GCGC'C
Cuts at: 742 5762 6361 6609 742
Size: 5020 599 248 3060
Fragments arranged by size:
5020 3060 599 248
BbsI GAAGACnn'nnnn_
Cuts at: 1088 2857 1088
Size: 1769 7158
BbvCI CC'TCA_GC
Cuts at: 205 1147 1236 1758 4961 205
Size: 942 89 522 3203 4171
Fragments arranged by size:
4171 3203 942 522 89
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1468 1502 3769 3803 1468
Size: 34 2267 34 6592
Fragments arranged by size:
6592 2267 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5691 6825 7881 8927 5691
Size: 1134 1056 1046 5691
Fragments arranged by size:
5691 1134 1056 1046
BclI T'GATC_A
Cuts at: 6447 6447
Size: 8927
BfrBI ATG'CAT
Cuts at: 479 479
Size: 8927
BglI GCCn_nnn'nGGC
Cuts at: 2101 2705 2854 3218 4109 4863 5824 2101
Size: 604 149 364 891 754 961 5204
Fragments arranged by size:
5204 961 891 754 604 364 149
BglII A'GATC_T
Cuts at: 2649 6442 8479 2649
Size: 3793 2037 3097
Fragments arranged by size:
3793 3097 2037
BlpI GC'TnA_GC
Cuts at: 220 1009 1139 1949 2105 2152 3152 4564 6039
Size: 789 130 810 156 47 1000 1412 1475
Cuts at: 6039 220
Size: 3108
Fragments arranged by size:
3108 1475 1412 1000 810 789 156 130
47
Bme1580I G_kGCm'C
Cuts at: 362 1505 1585 1655 1699 1905 2972 3770 3792
Size: 1143 80 70 44 206 1067 798 22
Cuts at: 3792 3919 4077 4105 4332 4352 4767 5179 5356
Size: 127 158 28 227 20 415 412 177
Cuts at: 5356 5916 6552 6645 7769 362
Size: 560 636 93 1124 1520
Fragments arranged by size:
1520 1143 1124 1067 798 636 560 415
412 227 206 177 158 127 93 80
70 44 28 22 20
BmgBI CAC'GTC
Cuts at: 1890 3390 4762 5117 5157 5721 5831 1890
Size: 1500 1372 355 40 564 110 4986
Fragments arranged by size:
4986 1500 1372 564 355 110 40
BmrI ACTGGGnnnn_n'
Cuts at: 2130 2167 2297 2328 2555 3015 3110 3128 3363
Size: 37 130 31 227 460 95 18 235
Cuts at: 3363 3487 3916 4339 6139 6220 6555 8699 2130
Size: 124 429 423 1800 81 335 2144 2358
Fragments arranged by size:
2358 2144 1800 460 429 423 335 235
227 130 124 95 81 37 31 18
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4717 7294 4717
Size: 2577 6350
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 337 394 577 586 721 827 973 1687
Size: 21 57 183 9 135 106 146 714
Cuts at: 1687 1696 1940 2140 2404 2504 2980 3516 3793
Size: 9 244 200 264 100 476 536 277
Cuts at: 3793 4660 4705 316
Size: 867 45 4538
Fragments arranged by size:
4538 867 714 536 476 277 264 244
200 183 146 135 106 100 57 45
21 9 9
Bpu10I CC'TnA_GC
Cuts at: 205 873 1147 1236 1758 2069 2423 3568 4116
Size: 668 274 89 522 311 354 1145 548
Cuts at: 4116 4961 205
Size: 845 4171
Fragments arranged by size:
4171 1145 845 668 548 522 354 311
274 89
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4882 5878 6103 6982 7696 7994 4882
Size: 996 225 879 714 298 5815
Fragments arranged by size:
5815 996 879 714 298 225
BsaI GGTCTCn'nnnn_
Cuts at: 2480 2926 3511 3616 4383 4688 4945 5020 5539
Size: 446 585 105 767 305 257 75 519
Cuts at: 5539 8631 2480
Size: 3092 2776
Fragments arranged by size:
3092 2776 767 585 519 446 305 257
105 75
BsaAI yAC'GTr
Cuts at: 3765 3816 4372 6280 3765
Size: 51 556 1908 6412
Fragments arranged by size:
6412 1908 556 51
BsaBI GATnn'nnATC
Cuts at: 6 755 2476 6441 8478 6
Size: 749 1721 3965 2037 455
Fragments arranged by size:
3965 2037 1721 749 455
BsaHI Gr'CG_yC
Cuts at: 739 1429 3962 4030 5759 5873 6358 6606 7308
Size: 690 2533 68 1729 114 485 248 702
Cuts at: 7308 8804 739
Size: 1496 862
Fragments arranged by size:
2533 1729 1496 862 702 690 485 248
114 68
BsaWI w'CCGG_w
Cuts at: 528 1444 2141 5513 6116 6637 7315 7726 7873
Size: 916 697 3372 603 521 678 411 147
Cuts at: 7873 8147 528
Size: 274 1308
Fragments arranged by size:
3372 1308 916 697 678 603 521 411
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1261 1285 1291 1315 1669 1699 3442 3472 3473
Size: 24 6 24 354 30 1743 30 1
Cuts at: 3473 3503 5053 5083 5815 5845 1261
Size: 30 1550 30 732 30 4343
Fragments arranged by size:
4343 1743 1550 732 354 30 30 30
30 30 24 24 6 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 832 1269 2430 2482 2509 4147 4171 4657 4698
Size: 437 1161 52 27 1638 24 486 41
Cuts at: 4698 5182 832
Size: 484 4577
Fragments arranged by size:
4577 1638 1161 486 484 437 52 41
27 24
BseYI C'CCAG_C
Cuts at: 467 951 1329 1743 1896 2581 2678 3408 3856
Size: 484 378 414 153 685 97 730 448
Cuts at: 3856 4001 4351 4596 4627 4985 4990 5280 5340
Size: 145 350 245 31 358 5 290 60
Cuts at: 5340 6352 7110 7775 8183 467
Size: 1012 758 665 408 1211
Fragments arranged by size:
1211 1012 758 730 685 665 484 448
414 408 378 358 350 290 245 153
145 97 60 31 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 372 712 760 793 946 1612 2003 3427
Size: 218 340 48 33 153 666 391 1424
Cuts at: 3427 3820 4696 154
Size: 393 876 4385
Fragments arranged by size:
4385 1424 876 666 393 391 340 218
153 48 33
BsiEI CG_ry'CG
Cuts at: 104 3095 4303 4307 4651 6469 6515 7745 104
Size: 2991 1208 4 344 1818 46 1230 1286
Fragments arranged by size:
2991 1818 1286 1230 1208 344 46 4
BsiHKAI G_wGCw'C
Cuts at: 203 647 824 1253 1655 2287 2881 2972 2999
Size: 444 177 429 402 632 594 91 27
Cuts at: 2999 3706 3770 3826 4468 5941 6719 6909 7769
Size: 707 64 56 642 1473 778 190 860
Cuts at: 7769 203
Size: 1361
Fragments arranged by size:
1473 1361 860 778 707 642 632 594
444 429 402 190 177 91 64 56
27
BsmI GAATG_Cn'
Cuts at: 3363 3363
Size: 8927
BsmBI CGTCTCn'nnnn_
Cuts at: 3021 3922 4851 5586 3021
Size: 901 929 735 6362
Fragments arranged by size:
6362 929 901 735
Bsp1286I G_dGCh'C
Cuts at: 203 343 362 536 647 824 851 958 1145
Size: 140 19 174 111 177 27 107 187
Cuts at: 1145 1253 1505 1585 1655 1699 1750 1905 1979
Size: 108 252 80 70 44 51 155 74
Cuts at: 1979 2135 2287 2429 2881 2972 2999 3706 3770
Size: 156 152 142 452 91 27 707 64
Cuts at: 3770 3792 3826 3919 4077 4105 4332 4352 4468
Size: 22 34 93 158 28 227 20 116
Cuts at: 4468 4767 4986 5179 5261 5356 5916 5941 6552
Size: 299 219 193 82 95 560 25 611
Cuts at: 6552 6645 6719 6909 6971 7769 203
Size: 93 74 190 62 798 1361
Fragments arranged by size:
1361 798 707 611 560 452 299 252
227 219 193 190 187 177 174 158
156 155 152 142 140 116 111 108
107 95 93 93 91 82 80 74
74 70 64 62 51 44 34 28
27 27 25 22 20 19
BspEI T'CCGG_A
Cuts at: 1444 1444
Size: 8927
BspHI T'CATG_A
Cuts at: 7359 7359
Size: 8927
BspMI ACCTGCnnnn'nnnn_
Cuts at: 974 1953 2069 2206 2636 3425 3641 3812 4341
Size: 979 116 137 430 789 216 171 529
Cuts at: 4341 5405 5654 5977 6191 6493 6874 974
Size: 1064 249 323 214 302 381 3027
Fragments arranged by size:
3027 1064 979 789 529 430 381 323
302 249 216 214 171 137 116
BsrBI CCG'CTC
Cuts at: 50 150 2073 3091 3198 4749 5588 5808 7219
Size: 100 1923 1018 107 1551 839 220 1411
Cuts at: 7219 7273 8757 8922 50
Size: 54 1484 165 55
Fragments arranged by size:
1923 1551 1484 1411 1018 839 220 165
107 100 55 54
BsrDI GCAATG_nn'
Cuts at: 1842 2051 6839 1842
Size: 209 4788 3930
Fragments arranged by size:
4788 3930 209
BsrFI r'CCGG_y
Cuts at: 744 1307 1628 1910 1960 1983 3874 4493 4697
Size: 563 321 282 50 23 1891 619 204
Cuts at: 4697 4924 5513 6925 7106 744
Size: 227 589 1412 181 2565
Fragments arranged by size:
2565 1891 1412 619 589 563 321 282
227 204 181 50 23
BsrGI T'GTAC_A
Cuts at: 233 233
Size: 8927
BssSI C'ACGA_G
Cuts at: 1033 3912 4585 4740 7198 7906 1033
Size: 2879 673 155 2458 708 2054
Fragments arranged by size:
2879 2458 2054 708 673 155
BstAPI GCAn_nnn'nTGC
Cuts at: 783 6304 783
Size: 5521 3406
BstBI TT'CG_AA
Cuts at: 7288 7288
Size: 8927
BstEII G'GTnAC_C
Cuts at: 3189 3189
Size: 8927
BstXI CCAn_nnnn'nTGG
Cuts at: 351 1333 2187 2271 3028 3123 3756 5136 351
Size: 982 854 84 757 95 633 1380 4142
Fragments arranged by size:
4142 1380 982 854 757 633 95 84
BstYI r'GATC_y
Cuts at: 1 1721 1793 2649 4556 5952 6419 6442 6777
Size: 1720 72 856 1907 1396 467 23 335
Cuts at: 6777 7023 7329 7341 7427 8479 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1907 1720 1396 1052 856 467 449 335
306 246 86 72 23 12
Bsu36I CC'TnA_GG
Cuts at: 2492 2703 2852 4025 2492
Size: 211 149 1173 7394
Fragments arranged by size:
7394 1173 211 149
BtgI C'CryG_G
Cuts at: 127 1208 3095 3512 3863 4937 5145 5762 5819
Size: 1081 1887 417 351 1074 208 617 57
Cuts at: 5819 127
Size: 3235
Fragments arranged by size:
3235 1887 1081 1074 617 417 351 208
57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2002 4178 5711 6877 7058 7221 2002
Size: 2176 1533 1166 181 163 3708
Fragments arranged by size:
3708 2176 1533 1166 181 163
BtsI GCAGTG_nn'
Cuts at: 384 725 1139 1233 2163 3248 3304 6170 384
Size: 341 414 94 930 1085 56 2866 3141
Fragments arranged by size:
3141 2866 1085 930 414 341 94 56
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1211 1246 4803 4838 1211
Size: 35 3557 35 5300
Fragments arranged by size:
5300 3557 35 35
DraI TTT'AAA
Cuts at: 5926 5926
Size: 8927
DraIII CAC_nnn'GTG
Cuts at: 2351 4590 2351
Size: 2239 6688
DrdI GACnn_nn'nnGTC
Cuts at: 6633 7977 6633
Size: 1344 7583
EaeI y'GGCC_r
Cuts at: 124 353 674 1871 1916 1934 2753 3030 3092
Size: 229 321 1197 45 18 819 277 62
Cuts at: 3092 3876 3956 4014 4300 4428 4922 5433 5660
Size: 784 80 58 286 128 494 511 227
Cuts at: 5660 5816 6512 6686 7077 7104 8449 124
Size: 156 696 174 391 27 1345 602
Fragments arranged by size:
1345 1197 819 784 696 602 511 494
391 321 286 277 229 227 174 156
128 80 62 58 45 27 18
EagI C'GGCC_G
Cuts at: 3092 4300 6512 3092
Size: 1208 2212 5507
Fragments arranged by size:
5507 2212 1208
EarI CTCTTCn'nnn_
Cuts at: 830 1204 3013 4148 4894 5383 6950 7160 830
Size: 374 1809 1135 746 489 1567 210 2597
Fragments arranged by size:
2597 1809 1567 1135 746 489 374 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 793 1647 1900 2928 3579 5767 7135 7877 8023
Size: 854 253 1028 651 2188 1368 742 146
Cuts at: 8023 8737 793
Size: 714 983
Fragments arranged by size:
2188 1368 1028 983 854 742 714 651
253 146
EcoICRI GAG'CTC
Cuts at: 822 1251 4466 5939 822
Size: 429 3215 1473 3810
Fragments arranged by size:
3810 3215 1473 429
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 337 394 577 586 721 827 973 982
Size: 21 57 183 9 135 106 146 9
Cuts at: 982 1408 1687 1696 1940 2140 2404 2504 2926
Size: 426 279 9 244 200 264 100 422
Cuts at: 2926 2980 2994 3516 3793 3992 4660 4705 5082
Size: 54 14 522 277 199 668 45 377
Cuts at: 5082 5248 6752 7184 7537 8199 316
Size: 166 1504 432 353 662 1044
Fragments arranged by size:
1504 1044 668 662 522 432 426 422
377 353 279 277 264 244 200 199
183 166 146 135 106 100 57 54
45 21 14 9 9 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2308 2445 4024 5650 2308
Size: 137 1579 1626 5585
Fragments arranged by size:
5585 1626 1579 137
EcoO109I rG'GnC_Cy
Cuts at: 726 1038 1104 1502 1550 1901 1902 2076 2856
Size: 312 66 398 48 351 1 174 780
Cuts at: 2856 3232 4073 4074 4101 4102 4456 4615 5046
Size: 376 841 1 27 1 354 159 431
Cuts at: 5046 5124 5353 5394 6066 726
Size: 78 229 41 672 3587
Fragments arranged by size:
3587 841 780 672 431 398 376 354
351 312 229 174 159 78 66 48
41 27 1 1 1
EcoRI G'AATT_C
Cuts at: 5931 5931
Size: 8927
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1413 2760 4593 4923 6946 1413
Size: 1347 1833 330 2023 3394
Fragments arranged by size:
3394 2023 1833 1347 330
FspI TGC'GCA
Cuts at: 1288 3259 5623 5854 6708 1288
Size: 1971 2364 231 854 3507
Fragments arranged by size:
3507 2364 1971 854 231
FspAI rTGC'GCAy
Cuts at: 3259 3259
Size: 8927
HaeII r_GCGC'y
Cuts at: 445 742 1016 1830 4795 5607 5762 5904 6361
Size: 297 274 814 2965 812 155 142 457
Cuts at: 6361 6609 7839 8610 445
Size: 248 1230 771 762
Fragments arranged by size:
2965 1230 814 812 771 762 457 297
274 248 155 142
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1261 1285 1293 1317 1539 1571 1605 1637 2770
Size: 24 8 24 222 32 34 32 1133
Cuts at: 2770 2802 3510 3542 3632 3664 5548 5580 5590
Size: 32 708 32 90 32 1884 32 10
Cuts at: 5590 5622 5675 5707 5710 5742 5815 5847 6769
Size: 32 53 32 3 32 73 32 922
Cuts at: 6769 6799 6801 6831 1261
Size: 30 2 30 3357
Fragments arranged by size:
3357 1884 1133 922 708 222 90 73
53 34 32 32 32 32 32 32
32 32 32 32 30 30 24 24
10 8 3 2
HincII GTy'rAC
Cuts at: 3666 3801 5966 3666
Size: 135 2165 6627
Fragments arranged by size:
6627 2165 135
HindIII A'AGCT_T
Cuts at: 4575 4575
Size: 8927
KasI G'GCGC_C
Cuts at: 738 5758 6357 6605 738
Size: 5020 599 248 3060
Fragments arranged by size:
5020 3060 599 248
KpnI G_GTAC'C
Cuts at: 3005 5947 3005
Size: 2942 5985
MluI A'CGCG_T
Cuts at: 8360 8360
Size: 8927
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 914 2228 3356 3629 3773 7686 7870 914
Size: 1314 1128 273 144 3913 184 1971
Fragments arranged by size:
3913 1971 1314 1128 273 184 144
MscI TGG'CCA
Cuts at: 126 1873 1936 2755 3032 4016 5662 5818 6688
Size: 1747 63 819 277 984 1646 156 870
Cuts at: 6688 126
Size: 2365
Fragments arranged by size:
2365 1747 1646 984 870 819 277 156
63
MslI CAynn'nnrTG
Cuts at: 349 631 1588 1648 3253 3281 3544 3773 4276
Size: 282 957 60 1605 28 263 229 503
Cuts at: 4276 4360 4397 4742 6916 7043 349
Size: 84 37 345 2174 127 2233
Fragments arranged by size:
2233 2174 1605 957 503 345 282 263
229 127 84 60 37 28
MspA1I CmG'CkG
Cuts at: 315 498 792 869 951 1119 1333 1596 1665
Size: 183 294 77 82 168 214 263 69
Cuts at: 1665 2537 2845 3111 3156 3514 3773 4257 4478
Size: 872 308 266 45 358 259 484 221
Cuts at: 4478 4529 4563 4600 4653 4960 5328 5542 5784
Size: 51 34 37 53 307 368 214 242
Cuts at: 5784 6038 6352 6712 7496 7741 315
Size: 254 314 360 784 245 1501
Fragments arranged by size:
1501 872 784 484 368 360 358 314
308 307 294 266 263 259 254 245
242 221 214 214 183 168 82 77
69 53 51 45 37 34
NaeI GCC'GGC
Cuts at: 1309 1630 1962 1985 3876 4926 7108 1309
Size: 321 332 23 1891 1050 2182 3128
Fragments arranged by size:
3128 2182 1891 1050 332 321 23
NarI GG'CG_CC
Cuts at: 739 5759 6358 6606 739
Size: 5020 599 248 3060
Fragments arranged by size:
5020 3060 599 248
NcoI C'CATG_G
Cuts at: 127 1208 5145 127
Size: 1081 3937 3909
Fragments arranged by size:
3937 3909 1081
NgoMIV G'CCGG_C
Cuts at: 1307 1628 1960 1983 3874 4924 7106 1307
Size: 321 332 23 1891 1050 2182 3128
Fragments arranged by size:
3128 2182 1891 1050 332 321 23
NsiI A_TGCA'T
Cuts at: 481 481
Size: 8927
NspI r_CATG'y
Cuts at: 3243 4779 4801 5853 5980 6480 8083 3243
Size: 1536 22 1052 127 500 1603 4087
Fragments arranged by size:
4087 1603 1536 1052 500 127 22
PasI CC'CwG_GG
Cuts at: 362 2081 4945 362
Size: 1719 2864 4344
Fragments arranged by size:
4344 2864 1719
PciI A'CATG_T
Cuts at: 3239 8079 3239
Size: 4840 4087
PflMI CCAn_nnn'nTGG
Cuts at: 2316 3043 3339 3375 4638 5056 2316
Size: 727 296 36 1263 418 6187
Fragments arranged by size:
6187 1263 727 418 296 36
PfoI T'CCnGG_A
Cuts at: 620 1358 3968 5390 620
Size: 738 2610 1422 4157
Fragments arranged by size:
4157 2610 1422 738
PmeI GTTT'AAAC
Cuts at: 5926 5926
Size: 8927
PmlI CAC'GTG
Cuts at: 3765 3765
Size: 8927
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2122 2154 7373 7405 2122
Size: 32 5219 32 3644
Fragments arranged by size:
5219 3644 32 32
PpuMI rG'GwC_Cy
Cuts at: 726 1104 2076 3232 4456 5124 5394 726
Size: 378 972 1156 1224 668 270 4259
Fragments arranged by size:
4259 1224 1156 972 668 378 270
PshAI GACnn'nnGTC
Cuts at: 4653 4653
Size: 8927
PspOMI G'GGCC_C
Cuts at: 1501 1901 4073 4101 5352 1501
Size: 400 2172 28 1251 5076
Fragments arranged by size:
5076 2172 1251 400 28
PspXI vC'TCGA_Gb
Cuts at: 4979 4979
Size: 8927
PstI C_TGCA'G
Cuts at: 497 791 1124 2542 2649 5309 5456 5597 5974
Size: 294 333 1418 107 2660 147 141 377
Cuts at: 5974 6659 497
Size: 685 2765
Fragments arranged by size:
2765 2660 1418 685 377 333 294 147
141 107
PvuI CG_AT'CG
Cuts at: 104 4307 104
Size: 4203 4724
PvuII CAG'CTG
Cuts at: 498 792 869 951 1119 1333 1596 1665 3156
Size: 294 77 82 168 214 263 69 1491
Cuts at: 3156 3773 4257 4563 5328 5542 5784 6352 6712
Size: 617 484 306 765 214 242 568 360
Cuts at: 6712 498
Size: 2713
Fragments arranged by size:
2713 1491 765 617 568 484 360 306
294 263 242 214 214 168 82 77
69
RsrII CG'GwC_CG
Cuts at: 7122 7122
Size: 8927
SacI G_AGCT'C
Cuts at: 824 1253 4468 5941 824
Size: 429 3215 1473 3810
Fragments arranged by size:
3810 3215 1473 429
SacII CC_GC'GG
Cuts at: 3515 3515
Size: 8927
SalI G'TCGA_C
Cuts at: 5964 5964
Size: 8927
SanDI GG'GwC_CC
Cuts at: 726 2076 3232 726
Size: 1350 1156 6421
Fragments arranged by size:
6421 1350 1156
SapI GCTCTTCn'nnn_
Cuts at: 830 5383 6950 7160 830
Size: 4553 1567 210 2597
Fragments arranged by size:
4553 2597 1567 210
SbfI CC_TGCA'GG
Cuts at: 5597 5974 5597
Size: 377 8550
ScaI AGT'ACT
Cuts at: 706 2299 3025 8444 706
Size: 1593 726 5419 1189
Fragments arranged by size:
5419 1593 1189 726
SexAI A'CCwGG_T
Cuts at: 5168 5168
Size: 8927
SfcI C'TryA_G
Cuts at: 33 493 787 1120 1560 2538 2645 3474 5305
Size: 460 294 333 440 978 107 829 1831
Cuts at: 5305 5452 5593 5970 6655 7623 7814 33
Size: 147 141 377 685 968 191 1146
Fragments arranged by size:
1831 1146 978 968 829 685 460 440
377 333 294 191 147 141 107
SfiI GGCCn_nnn'nGGCC
Cuts at: 3218 5824 3218
Size: 2606 6321
SfoI GGC'GCC
Cuts at: 740 5760 6359 6607 740
Size: 5020 599 248 3060
Fragments arranged by size:
5020 3060 599 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8927
SgrAI Cr'CCGG_yG
Cuts at: 744 744
Size: 8927
SmaI CCC'GGG
Cuts at: 1693 2026 5949 1693
Size: 333 3923 4671
Fragments arranged by size:
4671 3923 333
SmlI C'TyrA_G
Cuts at: 4897 4979 5893 6082 6997 7711 7973 4897
Size: 82 914 189 915 714 262 5851
Fragments arranged by size:
5851 915 914 714 262 189 82
SnaBI TAC'GTA
Cuts at: 3816 3816
Size: 8927
SpeI A'CTAG_T
Cuts at: 67 4912 67
Size: 4845 4082
SphI G_CATG'C
Cuts at: 5853 5980 6480 5853
Size: 127 500 8300
Fragments arranged by size:
8300 500 127
StuI AGG'CCT
Cuts at: 916 1756 5668 916
Size: 840 3912 4175
Fragments arranged by size:
4175 3912 840
StyI C'CwwG_G
Cuts at: 127 1208 1397 1493 2936 5145 5382 5663 6061
Size: 1081 189 96 1443 2209 237 281 398
Cuts at: 6061 6415 127
Size: 354 2639
Fragments arranged by size:
2639 2209 1443 1081 398 354 281 237
189 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1343 2197 3208 4810 5254 6510 6643 7102 7311
Size: 854 1011 1602 444 1256 133 459 209
Cuts at: 7311 1343
Size: 2959
Fragments arranged by size:
2959 1602 1256 1011 854 459 444 209
133
TatI w'GTAC_w
Cuts at: 233 704 1712 2297 3023 5420 8442 233
Size: 471 1008 585 726 2397 3022 718
Fragments arranged by size:
3022 2397 1008 726 718 585 471
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1669 1701 3229 3261 3442 3473 3474 3505 5053
Size: 32 1528 32 181 31 1 31 1548
Cuts at: 5053 5085 5386 5418 8444 8476 1669
Size: 32 301 32 3026 32 2120
Fragments arranged by size:
3026 2120 1548 1528 301 181 32 32
32 32 32 31 31 1
Tth111I GACn'n_nGTC
Cuts at: 628 6724 628
Size: 6096 2831
XbaI T'CTAG_A
Cuts at: 5958 5958
Size: 8927
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 936 1356 3040 4009 4359 4635 4815 4998 5718
Size: 420 1684 969 350 276 180 183 720
Cuts at: 5718 6422 936
Size: 704 3441
Fragments arranged by size:
3441 1684 969 720 704 420 350 276
183 180
XhoI C'TCGA_G
Cuts at: 4979 4979
Size: 8927
XmaI C'CCGG_G
Cuts at: 1691 2024 5947 1691
Size: 333 3923 4671
Fragments arranged by size:
4671 3923 333
XmnI GAAnn'nnTTC
Cuts at: 3292 3292
Size: 8927
ZraI GAC'GTC
Cuts at: 1430 1430
Size: 8927
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AscI AvrII BaeI BmtI BsiWI BssHII BstZ17I
ClaI EcoRV FseI HpaI MfeI NdeI NheI NotI
NruI PacI PsiI PsrI SrfI SspI SwaI