Restriction map for
pF1KA0405
(Circular) (Six-base) MAPSORT of: pF1KA0405.seq Check: 8923 from: 1 to: 5093
pF1KA0405 5093 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AatII G_ACGT'C
Cuts at: 1708 1708
Size: 5093
AccI GT'mk_AC
Cuts at: 248 386 2131 248
Size: 138 1745 3210
Fragments arranged by size:
3210 1745 138
Acc65I G'GTAC_C
Cuts at: 2109 2109
Size: 5093
AclI AA'CG_TT
Cuts at: 4933 4933
Size: 5093
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 768 1033 2918 3350 4097 4365 175
Size: 593 265 1885 432 747 268 903
Fragments arranged by size:
1885 903 747 593 432 268 265
AflIII A'CryG_T
Cuts at: 2445 3549 4526 2445
Size: 1104 977 3012
Fragments arranged by size:
3012 1104 977
AgeI A'CCGG_T
Cuts at: 3481 3481
Size: 5093
AleI CACnn'nnGTG
Cuts at: 1468 1468
Size: 5093
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1040 1040
Size: 5093
AlwNI CAG_nnn'CTG
Cuts at: 2398 3965 2398
Size: 1567 3526
ApaLI G'TGCA_C
Cuts at: 376 3863 376
Size: 3487 1606
ApoI r'AATT_y
Cuts at: 412 808 2097 4446 4702 412
Size: 396 1289 2349 256 803
Fragments arranged by size:
2349 1289 803 396 256
AseI AT'TA_AT
Cuts at: 20 332 2009 20
Size: 312 1677 3104
Fragments arranged by size:
3104 1677 312
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5093
AvaI C'yCGr_G
Cuts at: 2113 2113
Size: 5093
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 750 783 750
Size: 33 5060
BamHI G'GATC_C
Cuts at: 287 1379 2118 287
Size: 1092 739 3262
Fragments arranged by size:
3262 1092 739
BanI G'GyrC_C
Cuts at: 778 1882 2109 2523 2771 2806 778
Size: 1104 227 414 248 35 3065
Fragments arranged by size:
3065 1104 414 248 227 35
BanII G_rGCy'C
Cuts at: 185 1562 1723 2107 3137 185
Size: 1377 161 384 1030 2141
Fragments arranged by size:
2141 1377 1030 384 161
BbeI G_GCGC'C
Cuts at: 2527 2775 2527
Size: 248 4845
BbsI GAAGACnn'nnnn_
Cuts at: 566 1628 566
Size: 1062 4031
BciVI GTATCCnnnnn_n'
Cuts at: 2991 3752 5093 2991
Size: 761 1341 2991
Fragments arranged by size:
2991 1341 761
BclI T'GATC_A
Cuts at: 869 2613 869
Size: 1744 3349
BglI GCCn_nnn'nGGC
Cuts at: 636 636
Size: 5093
BglII A'GATC_T
Cuts at: 2608 4645 2608
Size: 2037 3056
BlpI GC'TnA_GC
Cuts at: 1783 2205 1783
Size: 422 4671
Bme1580I G_kGCm'C
Cuts at: 380 781 2718 2811 3867 380
Size: 401 1937 93 1056 1606
Fragments arranged by size:
1937 1606 1056 401 93
BmrI ACTGGGnnnn_n'
Cuts at: 1347 2305 2386 2721 4865 1347
Size: 958 81 335 2144 1575
Fragments arranged by size:
2144 1575 958 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3460 3460
Size: 5093
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1927 2092 1927
Size: 165 4928
Bpu10I CC'TnA_GC
Cuts at: 619 1540 619
Size: 921 4172
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 787 968 2269 3148 3640 3938 787
Size: 181 1301 879 492 298 1942
Fragments arranged by size:
1942 1301 879 492 298 181
BsaI GGTCTCn'nnnn_
Cuts at: 4797 4797
Size: 5093
BsaAI yAC'GTr
Cuts at: 823 2446 823
Size: 1623 3470
BsaBI GATnn'nnATC
Cuts at: 6 674 2607 4644 6
Size: 668 1933 2037 455
Fragments arranged by size:
2037 1933 668 455
BsaHI Gr'CG_yC
Cuts at: 1705 2524 2772 3474 4970 1705
Size: 819 248 702 1496 1828
Fragments arranged by size:
1828 1496 819 702 248
BsaWI w'CCGG_w
Cuts at: 2282 2803 3481 3755 3902 4313 2282
Size: 521 678 274 147 411 3062
Fragments arranged by size:
3062 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 299 329 1224 1239 1254 1269 299
Size: 30 895 15 15 15 4123
Fragments arranged by size:
4123 895 30 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 833 833
Size: 5093
BseYI C'CCAG_C
Cuts at: 133 497 1221 2518 3276 3853 4349 133
Size: 364 724 1297 758 577 496 877
Fragments arranged by size:
1297 877 758 724 577 496 364
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 505 343
Size: 162 4931
BsiEI CG_ry'CG
Cuts at: 104 2635 2681 3889 104
Size: 2531 46 1208 1308
Fragments arranged by size:
2531 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 380 2078 2107 2885 3075 3867 380
Size: 1698 29 778 190 792 1606
Fragments arranged by size:
1698 1606 792 778 190 29
BsmBI CGTCTCn'nnnn_
Cuts at: 1941 1941
Size: 5093
Bsp1286I G_dGCh'C
Cuts at: 185 380 781 1562 1723 2078 2107 2718 2811
Size: 195 401 781 161 355 29 611 93
Cuts at: 2811 2885 3075 3137 3867 185
Size: 74 190 62 730 1411
Fragments arranged by size:
1411 781 730 611 401 355 195 190
161 93 74 62 29
BspEI T'CCGG_A
Cuts at: 4313 4313
Size: 5093
BspHI T'CATG_A
Cuts at: 4269 4269
Size: 5093
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2143 2357 2659 3040 2143
Size: 214 302 381 4196
Fragments arranged by size:
4196 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3385 3439 4923 5088 50
Size: 3335 54 1484 165 55
Fragments arranged by size:
3335 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3005 3005
Size: 5093
BsrFI r'CCGG_y
Cuts at: 1853 3091 3272 3481 1853
Size: 1238 181 209 3465
Fragments arranged by size:
3465 1238 209 181
BsrGI T'GTAC_A
Cuts at: 366 366
Size: 5093
BssSI C'ACGA_G
Cuts at: 717 3364 3722 717
Size: 2647 358 2088
Fragments arranged by size:
2647 2088 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2470 2470
Size: 5093
BstBI TT'CG_AA
Cuts at: 3454 3454
Size: 5093
BstXI CCAn_nnnn'nTGG
Cuts at: 207 207
Size: 5093
BstYI r'GATC_y
Cuts at: 1 287 1379 2118 2585 2608 2943 3189 4201
Size: 286 1092 739 467 23 335 246 1012
Cuts at: 4201 4287 4299 4645 1
Size: 86 12 346 449
Fragments arranged by size:
1092 1012 739 467 449 346 335 286
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 138 107
Size: 31 5062
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3043 3224 3387 3043
Size: 181 163 4749
Fragments arranged by size:
4749 181 163
BtsI GCAGTG_nn'
Cuts at: 630 1022 2076 2336 630
Size: 392 1054 260 3387
Fragments arranged by size:
3387 1054 392 260
DraI TTT'AAA
Cuts at: 2092 2092
Size: 5093
DraIII CAC_nnn'GTG
Cuts at: 128 128
Size: 5093
DrdI GACnn_nn'nnGTC
Cuts at: 2799 3657 2799
Size: 858 4235
EaeI y'GGCC_r
Cuts at: 1151 2678 2852 3243 3270 4615 1151
Size: 1527 174 391 27 1345 1629
Fragments arranged by size:
1629 1527 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2678 2678
Size: 5093
EarI CTCTTCn'nnn_
Cuts at: 509 1219 1444 3116 3326 509
Size: 710 225 1672 210 2276
Fragments arranged by size:
2276 1672 710 225 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1872 3301 3611 3757 4903 1872
Size: 1429 310 146 1146 2062
Fragments arranged by size:
2062 1429 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2105 2105
Size: 5093
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 768 1033 1927 2092 2918 3350 4097 4365
Size: 593 265 894 165 826 432 747 268
Cuts at: 4365 175
Size: 903
Fragments arranged by size:
903 894 826 747 593 432 268 265
165
EcoO109I rG'GnC_Cy
Cuts at: 566 1129 2232 566
Size: 563 1103 3427
Fragments arranged by size:
3427 1103 563
EcoRI G'AATT_C
Cuts at: 412 2097 412
Size: 1685 3408
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1253 3112 1253
Size: 1859 3234
FspI TGC'GCA
Cuts at: 2874 2874
Size: 5093
HaeII r_GCGC'y
Cuts at: 1821 2527 2775 3797 4776 1821
Size: 706 248 1022 979 2138
Fragments arranged by size:
2138 1022 979 706 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 520 552 966 988 998 1020 1549 1581 2935
Size: 32 414 22 10 22 529 32 1354
Cuts at: 2935 2965 2967 2997 520
Size: 30 2 30 2616
Fragments arranged by size:
2616 1354 529 414 32 32 30 30
22 22 10 2
HincII GTy'rAC
Cuts at: 1551 2132 1551
Size: 581 4512
HindIII A'AGCT_T
Cuts at: 264 508 264
Size: 244 4849
HpaI GTT'AAC
Cuts at: 1551 1551
Size: 5093
KasI G'GCGC_C
Cuts at: 2523 2771 2523
Size: 248 4845
KpnI G_GTAC'C
Cuts at: 2113 2113
Size: 5093
MfeI C'AATT_G
Cuts at: 815 815
Size: 5093
MluI A'CGCG_T
Cuts at: 4526 4526
Size: 5093
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 719 983 1031 1267 1324 3764 3948 719
Size: 264 48 236 57 2440 184 1864
Fragments arranged by size:
2440 1864 264 236 184 57 48
MscI TGG'CCA
Cuts at: 2854 2854
Size: 5093
MslI CAynn'nnrTG
Cuts at: 527 1447 1468 3082 3209 527
Size: 920 21 1614 127 2411
Fragments arranged by size:
2411 1614 920 127 21
MspA1I CmG'CkG
Cuts at: 230 1427 1523 2204 2518 2878 3891 4136 230
Size: 1197 96 681 314 360 1013 245 1187
Fragments arranged by size:
1197 1187 1013 681 360 314 245 96
NaeI GCC'GGC
Cuts at: 1855 3274 1855
Size: 1419 3674
NarI GG'CG_CC
Cuts at: 2524 2772 2524
Size: 248 4845
NcoI C'CATG_G
Cuts at: 107 138 107
Size: 31 5062
NdeI CA'TA_TG
Cuts at: 1802 1802
Size: 5093
NgoMIV G'CCGG_C
Cuts at: 1853 3272 1853
Size: 1419 3674
NspI r_CATG'y
Cuts at: 2043 2146 2646 3553 2043
Size: 103 500 907 3583
Fragments arranged by size:
3583 907 500 103
PasI CC'CwG_GG
Cuts at: 177 177
Size: 5093
PciI A'CATG_T
Cuts at: 3549 3549
Size: 5093
PflMI CCAn_nnn'nTGG
Cuts at: 128 1716 128
Size: 1588 3505
PfoI T'CCnGG_A
Cuts at: 290 572 1904 290
Size: 282 1332 3479
Fragments arranged by size:
3479 1332 282
PmeI GTTT'AAAC
Cuts at: 2092 2092
Size: 5093
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4232 4264 4232
Size: 32 5061
PpuMI rG'GwC_Cy
Cuts at: 1129 1129
Size: 5093
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 232 264 232
Size: 32 5061
PstI C_TGCA'G
Cuts at: 354 1057 1982 2140 2825 354
Size: 703 925 158 685 2622
Fragments arranged by size:
2622 925 703 685 158
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5093
PvuII CAG'CTG
Cuts at: 1427 2518 2878 1427
Size: 1091 360 3642
Fragments arranged by size:
3642 1091 360
RsrII CG'GwC_CG
Cuts at: 3288 3288
Size: 5093
SacI G_AGCT'C
Cuts at: 2107 2107
Size: 5093
SalI G'TCGA_C
Cuts at: 2130 2130
Size: 5093
SapI GCTCTTCn'nnn_
Cuts at: 509 3116 3326 509
Size: 2607 210 2276
Fragments arranged by size:
2607 2276 210
SbfI CC_TGCA'GG
Cuts at: 2140 2140
Size: 5093
ScaI AGT'ACT
Cuts at: 4610 4610
Size: 5093
SfcI C'TryA_G
Cuts at: 33 115 350 1053 1978 2136 2821 3814 4005
Size: 82 235 703 925 158 685 993 191
Cuts at: 4005 33
Size: 1121
Fragments arranged by size:
1121 993 925 703 685 235 191 158
82
SfoI GGC'GCC
Cuts at: 2525 2773 2525
Size: 248 4845
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5093
SmaI CCC'GGG
Cuts at: 2115 2115
Size: 5093
SmlI C'TyrA_G
Cuts at: 802 983 2248 3163 3655 3917 802
Size: 181 1265 915 492 262 1978
Fragments arranged by size:
1978 1265 915 492 262 181
SnaBI TAC'GTA
Cuts at: 823 823
Size: 5093
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5093
SphI G_CATG'C
Cuts at: 2146 2646 2146
Size: 500 4593
SspI AAT'ATT
Cuts at: 468 468
Size: 5093
StyI C'CwwG_G
Cuts at: 107 138 1039 1125 2227 2581 107
Size: 31 901 86 1102 354 2619
Fragments arranged by size:
2619 1102 901 354 86 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1458 1663 2676 2809 3268 3477 1458
Size: 205 1013 133 459 209 3074
Fragments arranged by size:
3074 1013 459 209 205 133
TatI w'GTAC_w
Cuts at: 366 1231 4608 366
Size: 865 3377 851
Fragments arranged by size:
3377 865 851
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1239 1271 4610 4642 1239
Size: 32 3339 32 1690
Fragments arranged by size:
3339 1690 32 32
Tth111I GACn'n_nGTC
Cuts at: 1704 2890 1704
Size: 1186 3907
XbaI T'CTAG_A
Cuts at: 2124 2124
Size: 5093
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 546 552 2588 546
Size: 6 2036 3051
Fragments arranged by size:
3051 2036 6
XmaI C'CCGG_G
Cuts at: 2113 2113
Size: 5093
ZraI GAC'GTC
Cuts at: 1706 1706
Size: 5093
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AloI ApaI AscI AvrII
BbvCI BcgI BfrBI BmgBI BmtI BsiWI BsmI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI
FspAI NheI NotI NruI NsiI PacI PmlI PshAI
PsiI PspOMI PspXI SacII SanDI SexAI SfiI SgrAI
SrfI StuI SwaI XhoI XmnI