Restriction map for
pF1KA0406
(Circular) (Six-base) MAPSORT of: pF1KA0406.seq Check: 5988 from: 1 to: 6380
pF1KA0406 6380 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1918 1918
Size: 6380
AccI GT'mk_AC
Cuts at: 3418 3418
Size: 6380
Acc65I G'GTAC_C
Cuts at: 3396 3396
Size: 6380
AclI AA'CG_TT
Cuts at: 6220 6220
Size: 6380
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 235 307 1126 2546 2776 2779 4205 4637
Size: 9 72 819 1420 230 3 1426 432
Cuts at: 4637 5384 5652 226
Size: 747 268 954
Fragments arranged by size:
1426 1420 954 819 747 432 268 230
72 9 3
AflIII A'CryG_T
Cuts at: 361 3732 4836 5813 361
Size: 3371 1104 977 928
Fragments arranged by size:
3371 1104 977 928
AgeI A'CCGG_T
Cuts at: 4768 4768
Size: 6380
AhdI GACnn_n'nnGTC
Cuts at: 296 1227 296
Size: 931 5449
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 514 1265 514
Size: 751 5629
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2606 2638 2702 2734 2606
Size: 32 64 32 6252
Fragments arranged by size:
6252 64 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1705 2175 3075 3685 5252 1705
Size: 470 900 610 1567 2833
Fragments arranged by size:
2833 1567 900 610 470
ApaI G_GGCC'C
Cuts at: 1345 3082 1345
Size: 1737 4643
ApaLI G'TGCA_C
Cuts at: 5150 5150
Size: 6380
ApoI r'AATT_y
Cuts at: 1297 2187 3384 5733 5989 1297
Size: 890 1197 2349 256 1688
Fragments arranged by size:
2349 1688 1197 890 256
AseI AT'TA_AT
Cuts at: 20 496 20
Size: 476 5904
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6380
AvaI C'yCGr_G
Cuts at: 3400 3400
Size: 6380
AvrII C'CTAG_G
Cuts at: 3102 3102
Size: 6380
BamHI G'GATC_C
Cuts at: 3405 3405
Size: 6380
BanI G'GyrC_C
Cuts at: 2441 3396 3810 4058 4093 2441
Size: 955 414 248 35 4728
Fragments arranged by size:
4728 955 414 248 35
BanII G_rGCy'C
Cuts at: 542 1345 3082 3394 4424 542
Size: 803 1737 312 1030 2498
Fragments arranged by size:
2498 1737 1030 803 312
BbeI G_GCGC'C
Cuts at: 3814 4062 3814
Size: 248 6132
BbsI GAAGACnn'nnnn_
Cuts at: 295 2534 3189 295
Size: 2239 655 3486
Fragments arranged by size:
3486 2239 655
BbvCI CC'TCA_GC
Cuts at: 1494 1494
Size: 6380
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3330 3364 3330
Size: 34 6346
BciVI GTATCCnnnnn_n'
Cuts at: 4278 5039 6380 4278
Size: 761 1341 4278
Fragments arranged by size:
4278 1341 761
BclI T'GATC_A
Cuts at: 2177 3900 2177
Size: 1723 4657
BfrBI ATG'CAT
Cuts at: 1991 1991
Size: 6380
BglI GCCn_nnn'nGGC
Cuts at: 2878 2878
Size: 6380
BglII A'GATC_T
Cuts at: 818 3895 5932 818
Size: 3077 2037 1266
Fragments arranged by size:
3077 2037 1266
BlpI GC'TnA_GC
Cuts at: 3492 3492
Size: 6380
Bme1580I G_kGCm'C
Cuts at: 240 1345 2444 3082 4005 4098 5154 240
Size: 1105 1099 638 923 93 1056 1466
Fragments arranged by size:
1466 1105 1099 1056 923 638 93
BmgBI CAC'GTC
Cuts at: 1425 1895 2703 1425
Size: 470 808 5102
Fragments arranged by size:
5102 808 470
BmrI ACTGGGnnnn_n'
Cuts at: 1149 2406 3592 3673 4008 6152 1149
Size: 1257 1186 81 335 2144 1377
Fragments arranged by size:
2144 1377 1257 1186 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4747 4747
Size: 6380
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 1366 1735 2098 2108 3337 370
Size: 996 369 363 10 1229 3413
Fragments arranged by size:
3413 1229 996 369 363 10
Bpu10I CC'TnA_GC
Cuts at: 1494 1494
Size: 6380
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1590 2524 3346 3556 4435 4927 5225 1590
Size: 934 822 210 879 492 298 2745
Fragments arranged by size:
2745 934 879 822 492 298 210
BsaI GGTCTCn'nnnn_
Cuts at: 2084 6084 2084
Size: 4000 2380
BsaAI yAC'GTr
Cuts at: 2908 3733 2908
Size: 825 5555
BsaBI GATnn'nnATC
Cuts at: 6 2738 3894 5931 6
Size: 2732 1156 2037 455
Fragments arranged by size:
2732 2037 1156 455
BsaHI Gr'CG_yC
Cuts at: 2870 3811 4059 4761 6257 2870
Size: 941 248 702 1496 2993
Fragments arranged by size:
2993 1496 941 702 248
BsaWI w'CCGG_w
Cuts at: 1661 3569 4090 4768 5042 5189 5600 1661
Size: 1908 521 678 274 147 411 2441
Fragments arranged by size:
2441 1908 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3211 3241 3211
Size: 30 6350
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 145 472 1539 1870 145
Size: 327 1067 331 4655
Fragments arranged by size:
4655 1067 331 327
BseYI C'CCAG_C
Cuts at: 427 711 1708 3286 3805 4563 5140 5636 427
Size: 284 997 1578 519 758 577 496 1171
Fragments arranged by size:
1578 1171 997 758 577 519 496 284
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 737 1089 3286 3292 3322 737
Size: 352 2197 6 30 3795
Fragments arranged by size:
3795 2197 352 30 6
BsiEI CG_ry'CG
Cuts at: 104 3922 3968 5176 104
Size: 3818 46 1208 1308
Fragments arranged by size:
3818 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 905 1874 2515 3353 3394 4172 4362 5154 905
Size: 969 641 838 41 778 190 792 2131
Fragments arranged by size:
2131 969 838 792 778 641 190 41
BsmI GAATG_Cn'
Cuts at: 340 1242 1482 1991 340
Size: 902 240 509 4729
Fragments arranged by size:
4729 902 509 240
Bsp1286I G_dGCh'C
Cuts at: 240 542 905 1345 1874 2444 2515 3082 3353
Size: 302 363 440 529 570 71 567 271
Cuts at: 3353 3394 4005 4098 4172 4362 4424 5154 240
Size: 41 611 93 74 190 62 730 1466
Fragments arranged by size:
1466 730 611 570 567 529 440 363
302 271 190 93 74 71 62 41
BspEI T'CCGG_A
Cuts at: 5600 5600
Size: 6380
BspHI T'CATG_A
Cuts at: 5556 5556
Size: 6380
BspMI ACCTGCnnnn'nnnn_
Cuts at: 450 900 1566 1918 2303 3363 3430 3644 3946
Size: 450 666 352 385 1060 67 214 302
Cuts at: 3946 4327 450
Size: 381 2503
Fragments arranged by size:
2503 1060 666 450 385 381 352 302
214 67
BsrBI CCG'CTC
Cuts at: 50 4672 4726 6210 6375 50
Size: 4622 54 1484 165 55
Fragments arranged by size:
4622 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2677 4292 2677
Size: 1615 4765
BsrFI r'CCGG_y
Cuts at: 2945 4378 4559 4768 2945
Size: 1433 181 209 4557
Fragments arranged by size:
4557 1433 209 181
BssSI C'ACGA_G
Cuts at: 4651 5009 4651
Size: 358 6022
BstAPI GCAn_nnn'nTGC
Cuts at: 892 3757 892
Size: 2865 3515
BstBI TT'CG_AA
Cuts at: 4741 4741
Size: 6380
BstEII G'GTnAC_C
Cuts at: 1824 3112 1824
Size: 1288 5092
BstYI r'GATC_y
Cuts at: 1 818 1725 2210 2684 2775 3405 3872 3895
Size: 817 907 485 474 91 630 467 23
Cuts at: 3895 4230 4476 5488 5574 5586 5932 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 907 817 630 485 474 467 449
346 335 246 91 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 128 128
Size: 6380
BtgI C'CryG_G
Cuts at: 107 2696 107
Size: 2589 3791
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4330 4511 4674 4330
Size: 181 163 6036
Fragments arranged by size:
6036 181 163
BtsI GCAGTG_nn'
Cuts at: 664 2671 2888 3623 664
Size: 2007 217 735 3421
Fragments arranged by size:
3421 2007 735 217
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2660 2695 2660
Size: 35 6345
DraI TTT'AAA
Cuts at: 783 2219 3379 783
Size: 1436 1160 3784
Fragments arranged by size:
3784 1436 1160
DraIII CAC_nnn'GTG
Cuts at: 3300 3300
Size: 6380
DrdI GACnn_nn'nnGTC
Cuts at: 4086 4944 4086
Size: 858 5522
EaeI y'GGCC_r
Cuts at: 2010 2333 3965 4139 4530 4557 5902 2010
Size: 323 1632 174 391 27 1345 2488
Fragments arranged by size:
2488 1632 1345 391 323 174 27
EagI C'GGCC_G
Cuts at: 3965 3965
Size: 6380
EarI CTCTTCn'nnn_
Cuts at: 271 1265 2462 2503 2603 4403 4613 271
Size: 994 1197 41 100 1800 210 2038
Fragments arranged by size:
2038 1800 1197 994 210 100 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2740 4588 4898 5044 6190 2740
Size: 1848 310 146 1146 2930
Fragments arranged by size:
2930 1848 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3392 3392
Size: 6380
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 235 307 370 1126 1366 1735 2098 2108
Size: 9 72 63 756 240 369 363 10
Cuts at: 2108 2546 2776 2779 3337 4205 4637 5384 5652
Size: 438 230 3 558 868 432 747 268
Cuts at: 5652 226
Size: 954
Fragments arranged by size:
954 868 756 747 558 438 432 369
363 268 240 230 72 63 10 9
3
EcoNI CCTnn'n_nnAGG
Cuts at: 1105 3106 1105
Size: 2001 4379
EcoO109I rG'GnC_Cy
Cuts at: 298 1053 2259 2997 3072 3079 3519 298
Size: 755 1206 738 75 7 440 3159
Fragments arranged by size:
3159 1206 755 738 440 75 7
EcoRI G'AATT_C
Cuts at: 3384 3384
Size: 6380
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4399 4399
Size: 6380
FspI TGC'GCA
Cuts at: 4161 4161
Size: 6380
HaeII r_GCGC'y
Cuts at: 3814 4062 5084 6063 3814
Size: 248 1022 979 4131
Fragments arranged by size:
4131 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 282 314 1213 1245 2602 2634 3211 3243 4222
Size: 32 899 32 1357 32 577 32 979
Cuts at: 4222 4252 4254 4284 282
Size: 30 2 30 2378
Fragments arranged by size:
2378 1357 979 899 577 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 968 3419 968
Size: 2451 3929
KasI G'GCGC_C
Cuts at: 3810 4058 3810
Size: 248 6132
KpnI G_GTAC'C
Cuts at: 3400 3400
Size: 6380
MluI A'CGCG_T
Cuts at: 5813 5813
Size: 6380
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1438 1979 5051 5235 1438
Size: 541 3072 184 2583
Fragments arranged by size:
3072 2583 541 184
MscI TGG'CCA
Cuts at: 2012 2335 4141 2012
Size: 323 1806 4251
Fragments arranged by size:
4251 1806 323
MslI CAynn'nnrTG
Cuts at: 496 2131 2701 4369 4496 496
Size: 1635 570 1668 127 2380
Fragments arranged by size:
2380 1668 1635 570 127
MspA1I CmG'CkG
Cuts at: 711 1386 2524 2814 3057 3306 3320 3357 3491
Size: 675 1138 290 243 249 14 37 134
Cuts at: 3491 3805 4165 5178 5423 711
Size: 314 360 1013 245 1668
Fragments arranged by size:
1668 1138 1013 675 360 314 290 249
245 243 134 37 14
NaeI GCC'GGC
Cuts at: 4561 4561
Size: 6380
NarI GG'CG_CC
Cuts at: 3811 4059 3811
Size: 248 6132
NcoI C'CATG_G
Cuts at: 107 2696 107
Size: 2589 3791
NdeI CA'TA_TG
Cuts at: 2022 2022
Size: 6380
NgoMIV G'CCGG_C
Cuts at: 4559 4559
Size: 6380
NsiI A_TGCA'T
Cuts at: 1993 1993
Size: 6380
NspI r_CATG'y
Cuts at: 365 2392 3433 3933 4840 365
Size: 2027 1041 500 907 1905
Fragments arranged by size:
2027 1905 1041 907 500
PasI CC'CwG_GG
Cuts at: 2913 2913
Size: 6380
PciI A'CATG_T
Cuts at: 361 4836 361
Size: 4475 1905
PflMI CCAn_nnn'nTGG
Cuts at: 186 327 186
Size: 141 6239
PfoI T'CCnGG_A
Cuts at: 386 2261 386
Size: 1875 4505
PmeI GTTT'AAAC
Cuts at: 3379 3379
Size: 6380
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5519 5551 5519
Size: 32 6348
PpuMI rG'GwC_Cy
Cuts at: 298 1053 2259 298
Size: 755 1206 4419
Fragments arranged by size:
4419 1206 755
PshAI GACnn'nnGTC
Cuts at: 2651 2651
Size: 6380
PspOMI G'GGCC_C
Cuts at: 1341 3078 1341
Size: 1737 4643
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3238 3270 3238
Size: 32 6348
PstI C_TGCA'G
Cuts at: 209 897 2171 3374 3427 4112 209
Size: 688 1274 1203 53 685 2477
Fragments arranged by size:
2477 1274 1203 688 685 53
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6380
PvuII CAG'CTG
Cuts at: 711 2524 2814 3057 3306 3357 3805 4165 711
Size: 1813 290 243 249 51 448 360 2926
Fragments arranged by size:
2926 1813 448 360 290 249 243 51
RsrII CG'GwC_CG
Cuts at: 4575 4575
Size: 6380
SacI G_AGCT'C
Cuts at: 3394 3394
Size: 6380
SalI G'TCGA_C
Cuts at: 3417 3417
Size: 6380
SapI GCTCTTCn'nnn_
Cuts at: 2503 4403 4613 2503
Size: 1900 210 4270
Fragments arranged by size:
4270 1900 210
SbfI CC_TGCA'GG
Cuts at: 3427 3427
Size: 6380
ScaI AGT'ACT
Cuts at: 2075 5897 2075
Size: 3822 2558
SexAI A'CCwGG_T
Cuts at: 3232 3232
Size: 6380
SfcI C'TryA_G
Cuts at: 33 205 893 2167 3370 3423 4108 5101 5292
Size: 172 688 1274 1203 53 685 993 191
Cuts at: 5292 33
Size: 1121
Fragments arranged by size:
1274 1203 1121 993 688 685 191 172
53
SfoI GGC'GCC
Cuts at: 3812 4060 3812
Size: 248 6132
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6380
SmaI CCC'GGG
Cuts at: 3402 3402
Size: 6380
SmlI C'TyrA_G
Cuts at: 1569 2503 3361 3535 4450 4942 5204 1569
Size: 934 858 174 915 492 262 2745
Fragments arranged by size:
2745 934 915 858 492 262 174
SnaBI TAC'GTA
Cuts at: 2908 2908
Size: 6380
SpeI A'CTAG_T
Cuts at: 67 1122 67
Size: 1055 5325
SphI G_CATG'C
Cuts at: 2392 3433 3933 2392
Size: 1041 500 4839
Fragments arranged by size:
4839 1041 500
StuI AGG'CCT
Cuts at: 136 587 1111 1880 2836 136
Size: 451 524 769 956 3680
Fragments arranged by size:
3680 956 769 524 451
StyI C'CwwG_G
Cuts at: 107 679 1507 2696 2822 3102 3514 3868 107
Size: 572 828 1189 126 280 412 354 2619
Fragments arranged by size:
2619 1189 828 572 412 354 280 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 441 1025 1889 3007 3963 4096 4555 4764 441
Size: 584 864 1118 956 133 459 209 2057
Fragments arranged by size:
2057 1118 956 864 584 459 209 133
TatI w'GTAC_w
Cuts at: 257 2073 5895 257
Size: 1816 3822 742
Fragments arranged by size:
3822 1816 742
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1245 1277 5897 5929 1245
Size: 32 4620 32 1696
Fragments arranged by size:
4620 1696 32 32
Tth111I GACn'n_nGTC
Cuts at: 4177 4177
Size: 6380
XbaI T'CTAG_A
Cuts at: 1413 3411 1413
Size: 1998 4382
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2130 2703 3230 3875 2130
Size: 573 527 645 4635
Fragments arranged by size:
4635 645 573 527
XmaI C'CCGG_G
Cuts at: 3400 3400
Size: 6380
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AscI BaeI BmtI BsiWI
BsmBI BsrGI BssHII BstXI BstZ17I ClaI EcoRV FseI
FspAI HindIII HpaI MfeI NheI NotI NruI PacI
PmlI PsiI PspXI SacII SanDI SfiI SgrAI SrfI
SspI SwaI XhoI XmnI ZraI