Restriction map for
pF1KA0407
(Circular) (Six-base) MAPSORT of: pF1KA0407.seq Check: 7704 from: 1 to: 9518
pF1KA0407 9518 bp
With 182 enzymes: SgfI *
September 18, 2009 16:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1616 3055 4134 1616
Size: 1439 1079 7000
Fragments arranged by size:
7000 1439 1079
AccI GT'mk_AC
Cuts at: 1400 3353 3850 5194 6556 1400
Size: 1953 497 1344 1362 4362
Fragments arranged by size:
4362 1953 1362 1344 497
Acc65I G'GTAC_C
Cuts at: 6534 6534
Size: 9518
AclI AA'CG_TT
Cuts at: 9358 9358
Size: 9518
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1552 2424 7343 7775 8522 8790 1552
Size: 872 4919 432 747 268 2280
Fragments arranged by size:
4919 2280 872 747 432 268
AflIII A'CryG_T
Cuts at: 6870 7974 8951 6870
Size: 1104 977 7437
Fragments arranged by size:
7437 1104 977
AgeI A'CCGG_T
Cuts at: 7906 7906
Size: 9518
AhdI GACnn_n'nnGTC
Cuts at: 3559 6283 3559
Size: 2724 6794
AleI CACnn'nnGTG
Cuts at: 1791 3532 1791
Size: 1741 7777
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 226 1475 2859 2941 226
Size: 1249 1384 82 6803
Fragments arranged by size:
6803 1384 1249 82
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5899 5931 5899
Size: 32 9486
AlwNI CAG_nnn'CTG
Cuts at: 219 341 498 1563 2051 2082 2312 2332 2521
Size: 122 157 1065 488 31 230 20 189
Cuts at: 2521 3200 3936 4440 4662 5149 5592 6487 6823
Size: 679 736 504 222 487 443 895 336
Cuts at: 6823 8390 219
Size: 1567 1347
Fragments arranged by size:
1567 1347 1065 895 736 679 504 488
487 443 336 230 222 189 157 122
31 20
ApaI G_GGCC'C
Cuts at: 124 429 672 1411 1666 2117 2749 124
Size: 305 243 739 255 451 632 6893
Fragments arranged by size:
6893 739 632 451 305 255 243
ApaLI G'TGCA_C
Cuts at: 448 1199 2085 8288 448
Size: 751 886 6203 1678
Fragments arranged by size:
6203 1678 886 751
ApoI r'AATT_y
Cuts at: 4168 4735 6522 8871 9127 4168
Size: 567 1787 2349 256 4559
Fragments arranged by size:
4559 2349 1787 567 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 9518
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9518
AvaI C'yCGr_G
Cuts at: 487 665 854 1133 1650 2527 2656 2800 2983
Size: 178 189 279 517 877 129 144 183
Cuts at: 2983 3002 3266 3978 5000 6352 6538 487
Size: 19 264 712 1022 1352 186 3467
Fragments arranged by size:
3467 1352 1022 877 712 517 279 264
189 186 183 178 144 129 19
BamHI G'GATC_C
Cuts at: 3588 6543 3588
Size: 2955 6563
BanI G'GyrC_C
Cuts at: 241 473 3361 3523 3540 3637 4403 4448 4467
Size: 232 2888 162 17 97 766 45 19
Cuts at: 4467 4594 4844 5546 5600 5759 5887 6534 6948
Size: 127 250 702 54 159 128 647 414
Cuts at: 6948 7196 7231 241
Size: 248 35 2528
Fragments arranged by size:
2888 2528 766 702 647 414 250 248
232 162 159 128 127 97 54 45
35 19 17
BanII G_rGCy'C
Cuts at: 124 286 421 429 599 672 1411 1666 1943
Size: 162 135 8 170 73 739 255 277
Cuts at: 1943 2117 2140 2253 2384 2608 2749 2807 2872
Size: 174 23 113 131 224 141 58 65
Cuts at: 2872 3092 3385 4424 4552 6532 7562 124
Size: 220 293 1039 128 1980 1030 2080
Fragments arranged by size:
2080 1980 1039 1030 739 293 277 255
224 220 174 170 162 141 135 131
128 113 73 65 58 23 8
BbeI G_GCGC'C
Cuts at: 477 3527 5604 6952 7200 477
Size: 3050 2077 1348 248 2795
Fragments arranged by size:
3050 2795 2077 1348 248
BbsI GAAGACnn'nnnn_
Cuts at: 4396 6043 4396
Size: 1647 7871
BbvCI CC'TCA_GC
Cuts at: 1148 2448 3143 3287 1148
Size: 1300 695 144 7379
Fragments arranged by size:
7379 1300 695 144
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 387 421 1106 1140 3969 4003 90
Size: 34 263 34 685 34 2829 34 5605
Fragments arranged by size:
5605 2829 685 263 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 613 1935 7416 8177 9518 613
Size: 1322 5481 761 1341 613
Fragments arranged by size:
5481 1341 1322 761 613
BclI T'GATC_A
Cuts at: 7038 7038
Size: 9518
BglI GCCn_nnn'nGGC
Cuts at: 513 724 4284 5017 5796 5819 513
Size: 211 3560 733 779 23 4212
Fragments arranged by size:
4212 3560 779 733 211 23
BglII A'GATC_T
Cuts at: 5855 6507 7033 9070 5855
Size: 652 526 2037 6303
Fragments arranged by size:
6303 2037 652 526
BlpI GC'TnA_GC
Cuts at: 280 1556 2305 3064 6630 280
Size: 1276 749 759 3566 3168
Fragments arranged by size:
3566 3168 1276 759 749
Bme1580I G_kGCm'C
Cuts at: 124 373 429 452 672 1203 1411 1666 1873
Size: 249 56 23 220 531 208 255 207
Cuts at: 1873 2089 2117 2510 2552 2749 3310 3443 3640
Size: 216 28 393 42 197 561 133 197
Cuts at: 3640 4597 4847 5411 5549 5890 7143 7236 8292
Size: 957 250 564 138 341 1253 93 1056
Cuts at: 8292 124
Size: 1350
Fragments arranged by size:
1350 1253 1056 957 564 561 531 393
341 255 250 249 220 216 208 207
197 197 138 133 93 56 42 28
23
BmgBI CAC'GTC
Cuts at: 956 3556 3910 3942 6102 956
Size: 2600 354 32 2160 4372
Fragments arranged by size:
4372 2600 2160 354 32
BmrI ACTGGGnnnn_n'
Cuts at: 1891 3027 3213 3297 3353 3683 5097 6730 6811
Size: 1136 186 84 56 330 1414 1633 81
Cuts at: 6811 7146 9290 1891
Size: 335 2144 2119
Fragments arranged by size:
2144 2119 1633 1414 1136 335 330 186
84 81 56
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 880 2400 5503 6327 7885 880
Size: 1520 3103 824 1558 2513
Fragments arranged by size:
3103 2513 1558 1520 824
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 118 151 319 1076 1321 2995 3008 3455 3459
Size: 33 168 757 245 1674 13 447 4
Cuts at: 3459 3703 4714 4723 4996 5726 5746 6445 6454
Size: 244 1011 9 273 730 20 699 9
Cuts at: 6454 118
Size: 3182
Fragments arranged by size:
3182 1674 1011 757 730 699 447 273
245 244 168 33 20 13 9 9
4
Bpu10I CC'TnA_GC
Cuts at: 1148 1694 2448 3143 3287 1148
Size: 546 754 695 144 7379
Fragments arranged by size:
7379 754 695 546 144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2761 5074 6694 7573 8065 8363 2761
Size: 2313 1620 879 492 298 3916
Fragments arranged by size:
3916 2313 1620 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1466 3477 3701 3754 3981 6341 9222 1466
Size: 2011 224 53 227 2360 2881 1762
Fragments arranged by size:
2881 2360 2011 1762 227 224 53
BsaAI yAC'GTr
Cuts at: 206 778 1923 4115 4530 5016 6871 206
Size: 572 1145 2192 415 486 1855 2853
Fragments arranged by size:
2853 2192 1855 1145 572 486 415
BsaBI GATnn'nnATC
Cuts at: 6 1229 5182 7032 9069 6
Size: 1223 3953 1850 2037 455
Fragments arranged by size:
3953 2037 1850 1223 455
BsaHI Gr'CG_yC
Cuts at: 474 3524 3952 4017 5601 6161 6949 7197 7899
Size: 3050 428 65 1584 560 788 248 702
Cuts at: 7899 9395 474
Size: 1496 597
Fragments arranged by size:
3050 1584 1496 788 702 597 560 428
248 65
BsaWI w'CCGG_w
Cuts at: 6343 6707 7228 7906 8180 8327 8738 6343
Size: 364 521 678 274 147 411 7123
Fragments arranged by size:
7123 678 521 411 364 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 868 898 1417 1447 2205 2235 3457 3487 3724
Size: 30 519 30 758 30 1222 30 237
Cuts at: 3724 3754 4115 4145 5212 5242 5422 5452 5630
Size: 30 361 30 1067 30 180 30 178
Cuts at: 5630 5660 868
Size: 30 4726
Fragments arranged by size:
4726 1222 1067 758 519 361 237 180
178 30 30 30 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 724 990 1762 1925 2392 2701 3526 4089 4093
Size: 266 772 163 467 309 825 563 4
Cuts at: 4093 4363 6442 724
Size: 270 2079 3800
Fragments arranged by size:
3800 2079 825 772 563 467 309 270
266 163 4
BseYI C'CCAG_C
Cuts at: 123 380 1045 1255 1698 2051 2110 2176 2392
Size: 257 665 210 443 353 59 66 216
Cuts at: 2392 3309 3414 3631 3994 4103 4914 5492 5993
Size: 917 105 217 363 109 811 578 501
Cuts at: 5993 6186 6288 6456 6943 7701 8278 8774 123
Size: 193 102 168 487 758 577 496 867
Fragments arranged by size:
917 867 811 758 665 578 577 501
496 487 443 363 353 257 217 216
210 193 168 109 105 102 66 59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 992 1375 1650 2911 3424 4516 4552 6150 992
Size: 383 275 1261 513 1092 36 1598 4360
Fragments arranged by size:
4360 1598 1261 1092 513 383 275 36
BsiEI CG_ry'CG
Cuts at: 104 3123 7060 7106 8314 104
Size: 3019 3937 46 1208 1308
Fragments arranged by size:
3937 3019 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 452 986 1203 1502 1525 1557 1619 1651 1943
Size: 534 217 299 23 32 62 32 292
Cuts at: 1943 2089 3092 5681 6134 6532 7310 7500 8292
Size: 146 1003 2589 453 398 778 190 792
Cuts at: 8292 452
Size: 1678
Fragments arranged by size:
2589 1678 1003 792 778 534 453 398
299 292 217 190 146 62 32 32
23
BsmI GAATG_Cn'
Cuts at: 187 647 3989 5367 187
Size: 460 3342 1378 4338
Fragments arranged by size:
4338 3342 1378 460
BsmBI CGTCTCn'nnnn_
Cuts at: 1744 3649 4027 1744
Size: 1905 378 7235
Fragments arranged by size:
7235 1905 378
Bsp1286I G_dGCh'C
Cuts at: 124 286 373 421 429 452 599 672 986
Size: 162 87 48 8 23 147 73 314
Cuts at: 986 1203 1411 1502 1525 1557 1619 1651 1666
Size: 217 208 91 23 32 62 32 15
Cuts at: 1666 1873 1943 2089 2117 2140 2253 2384 2510
Size: 207 70 146 28 23 113 131 126
Cuts at: 2510 2552 2608 2749 2807 2872 3092 3310 3385
Size: 42 56 141 58 65 220 218 75
Cuts at: 3385 3443 3640 4424 4552 4597 4847 5411 5549
Size: 58 197 784 128 45 250 564 138
Cuts at: 5549 5681 5890 6134 6532 7143 7236 7310 7500
Size: 132 209 244 398 611 93 74 190
Cuts at: 7500 7562 8292 124
Size: 62 730 1350
Fragments arranged by size:
1350 784 730 611 564 398 314 250
244 220 218 217 209 208 207 197
190 162 147 146 141 138 132 131
128 126 113 93 91 87 75 74
73 70 65 62 62 58 58 56
48 45 42 32 32 28 23 23
23 15 8
BspEI T'CCGG_A
Cuts at: 6343 8738 6343
Size: 2395 7123
BspHI T'CATG_A
Cuts at: 1511 4748 8694 1511
Size: 3237 3946 2335
Fragments arranged by size:
3946 3237 2335
BspMI ACCTGCnnnn'nnnn_
Cuts at: 824 1616 1790 2317 2567 2933 3055 4134 4940
Size: 792 174 527 250 366 122 1079 806
Cuts at: 4940 5984 6568 6782 7084 7465 824
Size: 1044 584 214 302 381 2877
Fragments arranged by size:
2877 1079 1044 806 792 584 527 381
366 302 250 214 174 122
BsrBI CCG'CTC
Cuts at: 50 513 2898 3822 5712 5952 7810 7864 9348
Size: 463 2385 924 1890 240 1858 54 1484
Cuts at: 9348 9513 50
Size: 165 55
Fragments arranged by size:
2385 1890 1858 1484 924 463 240 165
55 54
BsrDI GCAATG_nn'
Cuts at: 4343 6140 7430 4343
Size: 1797 1290 6431
Fragments arranged by size:
6431 1797 1290
BsrFI r'CCGG_y
Cuts at: 316 1025 1097 2883 3118 3520 7516 7697 7906
Size: 709 72 1786 235 402 3996 181 209
Cuts at: 7906 316
Size: 1928
Fragments arranged by size:
3996 1928 1786 709 402 235 209 181
72
BsrGI T'GTAC_A
Cuts at: 4637 5228 4637
Size: 591 8927
BssHII G'CGCG_C
Cuts at: 5835 5835
Size: 9518
BssSI C'ACGA_G
Cuts at: 2100 2634 3484 7789 8147 2100
Size: 534 850 4305 358 3471
Fragments arranged by size:
4305 3471 850 534 358
BstAPI GCAn_nnn'nTGC
Cuts at: 341 498 3200 3411 4440 6895 341
Size: 157 2702 211 1029 2455 2964
Fragments arranged by size:
2964 2702 2455 1029 211 157
BstBI TT'CG_AA
Cuts at: 7879 7879
Size: 9518
BstEII G'GTnAC_C
Cuts at: 3966 3966
Size: 9518
BstXI CCAn_nnnn'nTGG
Cuts at: 966 1263 1673 1793 2208 3534 4006 6001 966
Size: 297 410 120 415 1326 472 1995 4483
Fragments arranged by size:
4483 1995 1326 472 415 410 297 120
BstYI r'GATC_y
Cuts at: 1 3588 4685 4829 5855 6507 6543 7010 7033
Size: 3587 1097 144 1026 652 36 467 23
Cuts at: 7033 7368 7614 8626 8712 8724 9070 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
3587 1097 1026 1012 652 467 449 346
335 246 144 86 36 23 12
BstZ17I GTA'TAC
Cuts at: 3851 5195 3851
Size: 1344 8174
Bsu36I CC'TnA_GG
Cuts at: 237 2459 4145 4262 4663 237
Size: 2222 1686 117 401 5092
Fragments arranged by size:
5092 2222 1686 401 117
BtgI C'CryG_G
Cuts at: 1262 1340 2117 2237 2320 2600 3176 3618 3815
Size: 78 777 120 83 280 576 442 197
Cuts at: 3815 3924 3971 4310 4427 4444 5033 1262
Size: 109 47 339 117 17 589 5747
Fragments arranged by size:
5747 777 589 576 442 339 280 197
120 117 109 83 78 47 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1344 1433 7468 7649 7812 1344
Size: 89 6035 181 163 3050
Fragments arranged by size:
6035 3050 181 163 89
BtsI GCAGTG_nn'
Cuts at: 734 1469 1678 2032 2510 4716 5038 5611 6416
Size: 735 209 354 478 2206 322 573 805
Cuts at: 6416 6761 734
Size: 345 3491
Fragments arranged by size:
3491 2206 805 735 573 478 354 345
322 209
DraI TTT'AAA
Cuts at: 6517 6517
Size: 9518
DraIII CAC_nnn'GTG
Cuts at: 762 3259 3305 3330 5259 5766 762
Size: 2497 46 25 1929 507 4514
Fragments arranged by size:
4514 2497 1929 507 46 25
DrdI GACnn_nn'nnGTC
Cuts at: 4016 4156 4186 5569 7224 8082 4016
Size: 140 30 1383 1655 858 5452
Fragments arranged by size:
5452 1655 1383 858 140 30
EaeI y'GGCC_r
Cuts at: 505 676 918 950 1265 1306 1422 1795 2210
Size: 171 242 32 315 41 116 373 415
Cuts at: 2210 3120 3296 3518 3927 5111 5497 5540 6173
Size: 910 176 222 409 1184 386 43 633
Cuts at: 6173 7103 7277 7668 7695 9040 505
Size: 930 174 391 27 1345 983
Fragments arranged by size:
1345 1184 983 930 910 633 415 409
391 386 373 315 242 222 176 174
171 116 43 41 32 27
EagI C'GGCC_G
Cuts at: 3120 7103 3120
Size: 3983 5535
EarI CTCTTCn'nnn_
Cuts at: 134 2540 2813 3543 4607 4642 4954 7541 7751
Size: 2406 273 730 1064 35 312 2587 210
Cuts at: 7751 134
Size: 1901
Fragments arranged by size:
2587 2406 1901 1064 730 312 273 210
35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2002 2677 7726 8036 8182 9328 2002
Size: 675 5049 310 146 1146 2192
Fragments arranged by size:
5049 2192 1146 675 310 146
EcoICRI GAG'CTC
Cuts at: 1941 3090 6530 1941
Size: 1149 3440 4929
Fragments arranged by size:
4929 3440 1149
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 118 151 319 1076 1321 1552 2424 2995 3008
Size: 33 168 757 245 231 872 571 13
Cuts at: 3008 3455 3459 3703 4714 4723 4996 5726 5746
Size: 447 4 244 1011 9 273 730 20
Cuts at: 5746 6445 6454 7343 7775 8522 8790 118
Size: 699 9 889 432 747 268 846
Fragments arranged by size:
1011 889 872 846 757 747 730 699
571 447 432 273 268 245 244 231
168 33 20 13 9 9 4
EcoNI CCTnn'n_nnAGG
Cuts at: 1087 2165 2935 4144 4462 5202 5387 5917 6427
Size: 1078 770 1209 318 740 185 530 510
Cuts at: 6427 1087
Size: 4178
Fragments arranged by size:
4178 1209 1078 770 740 530 510 318
185
EcoO109I rG'GnC_Cy
Cuts at: 156 228 425 426 669 813 1275 1490 1593
Size: 72 197 1 243 144 462 215 103
Cuts at: 1593 1662 1893 2463 2745 2746 2979 4149 4266
Size: 69 231 570 282 1 233 1170 117
Cuts at: 4266 4659 4869 5380 5418 5628 6657 156
Size: 393 210 511 38 210 1029 3017
Fragments arranged by size:
3017 1170 1029 570 511 462 393 282
243 233 231 215 210 210 197 144
117 103 72 69 38 1 1
EcoRI G'AATT_C
Cuts at: 4735 6522 4735
Size: 1787 7731
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3870 5985 7537 3870
Size: 2115 1552 5851
Fragments arranged by size:
5851 2115 1552
FseI GG_CCGG'CC
Cuts at: 1029 3122 1029
Size: 2093 7425
FspI TGC'GCA
Cuts at: 2024 5137 7299 2024
Size: 3113 2162 4243
Fragments arranged by size:
4243 3113 2162
HaeII r_GCGC'y
Cuts at: 477 791 1955 3316 3527 5604 6952 7200 8222
Size: 314 1164 1361 211 2077 1348 248 1022
Cuts at: 8222 9201 477
Size: 979 794
Fragments arranged by size:
2077 1361 1348 1164 1022 979 794 314
248 211
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 220 252 1343 1375 1558 1590 1909 1941 2713
Size: 32 1091 32 183 32 319 32 772
Cuts at: 2713 2745 3545 3577 3724 3756 4749 4781 5422
Size: 32 800 32 147 32 993 32 641
Cuts at: 5422 5454 5515 5547 6269 6301 7360 7390 7392
Size: 32 61 32 722 32 1059 30 2
Cuts at: 7392 7422 220
Size: 30 2316
Fragments arranged by size:
2316 1091 1059 993 800 772 722 641
319 183 147 61 32 32 32 32
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 4095 6557 4095
Size: 2462 7056
HindIII A'AGCT_T
Cuts at: 5050 5050
Size: 9518
KasI G'GCGC_C
Cuts at: 473 3523 5600 6948 7196 473
Size: 3050 2077 1348 248 2795
Fragments arranged by size:
3050 2795 2077 1348 248
KpnI G_GTAC'C
Cuts at: 6538 6538
Size: 9518
MluI A'CGCG_T
Cuts at: 8951 8951
Size: 9518
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3410 3684 6035 8189 8373 3410
Size: 274 2351 2154 184 4555
Fragments arranged by size:
4555 2351 2154 274 184
MscI TGG'CCA
Cuts at: 952 1267 1308 1424 1797 2212 3298 3929 5113
Size: 315 41 116 373 415 1086 631 1184
Cuts at: 5113 7279 952
Size: 2166 3191
Fragments arranged by size:
3191 2166 1184 1086 631 415 373 315
116 41
MslI CAynn'nnrTG
Cuts at: 1505 1791 2878 3532 3694 7507 7634 1505
Size: 286 1087 654 162 3813 127 3389
Fragments arranged by size:
3813 3389 1087 654 286 162 127
MspA1I CmG'CkG
Cuts at: 279 297 405 471 495 946 1206 1281 1327
Size: 18 108 66 24 451 260 75 46
Cuts at: 1327 1389 2051 2419 2481 3200 3532 3620 4692
Size: 62 662 368 62 719 332 88 1072
Cuts at: 4692 4724 4790 5496 6456 6484 6629 6943 7303
Size: 32 66 706 960 28 145 314 360
Cuts at: 7303 8316 8561 279
Size: 1013 245 1236
Fragments arranged by size:
1236 1072 1013 960 719 706 662 451
368 360 332 314 260 245 145 108
88 75 66 66 62 62 46 32
28 24 18
NaeI GCC'GGC
Cuts at: 1027 3120 3522 7699 1027
Size: 2093 402 4177 2846
Fragments arranged by size:
4177 2846 2093 402
NarI GG'CG_CC
Cuts at: 474 3524 5601 6949 7197 474
Size: 3050 2077 1348 248 2795
Fragments arranged by size:
3050 2795 2077 1348 248
NcoI C'CATG_G
Cuts at: 1262 1340 2117 2320 2600 1262
Size: 78 777 203 280 8180
Fragments arranged by size:
8180 777 280 203 78
NgoMIV G'CCGG_C
Cuts at: 1025 3118 3520 7697 1025
Size: 2093 402 4177 2846
Fragments arranged by size:
4177 2846 2093 402
NspI r_CATG'y
Cuts at: 3157 3403 4043 5235 5677 6571 7071 7978 3157
Size: 246 640 1192 442 894 500 907 4697
Fragments arranged by size:
4697 1192 907 894 640 500 442 246
PasI CC'CwG_GG
Cuts at: 286 421 579 1411 2312 2593 4154 4521 4552
Size: 135 158 832 901 281 1561 367 31
Cuts at: 4552 4840 5400 286
Size: 288 560 4404
Fragments arranged by size:
4404 1561 901 832 560 367 288 281
158 135 31
PciI A'CATG_T
Cuts at: 7974 7974
Size: 9518
PflMI CCAn_nnn'nTGG
Cuts at: 585 1603 3330 3343 3936 4974 5109 6186 585
Size: 1018 1727 13 593 1038 135 1077 3917
Fragments arranged by size:
3917 1727 1077 1038 1018 593 135 13
PfoI T'CCnGG_A
Cuts at: 866 2936 4028 5681 6331 866
Size: 2070 1092 1653 650 4053
Fragments arranged by size:
4053 2070 1653 1092 650
PmeI GTTT'AAAC
Cuts at: 6517 6517
Size: 9518
PmlI CAC'GTG
Cuts at: 778 4115 4530 778
Size: 3337 415 5766
Fragments arranged by size:
5766 3337 415
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8657 8689 8657
Size: 32 9486
PpuMI rG'GwC_Cy
Cuts at: 156 228 813 1275 1490 1593 2463 4149 4266
Size: 72 585 462 215 103 870 1686 117
Cuts at: 4266 4869 5418 5628 156
Size: 603 549 210 4046
Fragments arranged by size:
4046 1686 870 603 585 549 462 215
210 117 103 72
PshAI GACnn'nnGTC
Cuts at: 3973 3973
Size: 9518
PsiI TTA'TAA
Cuts at: 5474 5474
Size: 9518
PspOMI G'GGCC_C
Cuts at: 120 425 668 1407 1662 2113 2745 120
Size: 305 243 739 255 451 632 6893
Fragments arranged by size:
6893 739 632 451 305 255 243
PspXI vC'TCGA_Gb
Cuts at: 487 1650 2983 487
Size: 1163 1333 7022
Fragments arranged by size:
7022 1333 1163
PstI C_TGCA'G
Cuts at: 215 296 821 945 1464 2517 3083 3196 3992
Size: 81 525 124 519 1053 566 113 796
Cuts at: 3992 4063 4250 5618 5897 6565 7250 215
Size: 71 187 1368 279 668 685 2483
Fragments arranged by size:
2483 1368 1053 796 685 668 566 525
519 279 187 124 113 81 71
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9518
PvuII CAG'CTG
Cuts at: 279 297 405 495 946 1206 1327 1389 4692
Size: 18 108 90 451 260 121 62 3303
Cuts at: 4692 6456 6484 6943 7303 279
Size: 1764 28 459 360 2494
Fragments arranged by size:
3303 2494 1764 459 451 360 260 121
108 90 62 28 18
RsrII CG'GwC_CG
Cuts at: 7713 7713
Size: 9518
SacI G_AGCT'C
Cuts at: 1943 3092 6532 1943
Size: 1149 3440 4929
Fragments arranged by size:
4929 3440 1149
SacII CC_GC'GG
Cuts at: 3621 3621
Size: 9518
SalI G'TCGA_C
Cuts at: 6555 6555
Size: 9518
SanDI GG'GwC_CC
Cuts at: 228 1275 1593 228
Size: 1047 318 8153
Fragments arranged by size:
8153 1047 318
SapI GCTCTTCn'nnn_
Cuts at: 134 2540 4642 4954 7541 7751 134
Size: 2406 2102 312 2587 210 1901
Fragments arranged by size:
2587 2406 2102 1901 312 210
SbfI CC_TGCA'GG
Cuts at: 821 6565 821
Size: 5744 3774
ScaI AGT'ACT
Cuts at: 5965 6400 9035 5965
Size: 435 2635 6448
Fragments arranged by size:
6448 2635 435
SexAI A'CCwGG_T
Cuts at: 4184 5093 5762 4184
Size: 909 669 7940
Fragments arranged by size:
7940 909 669
SfcI C'TryA_G
Cuts at: 33 211 292 817 941 1460 2513 3079 3192
Size: 178 81 525 124 519 1053 566 113
Cuts at: 3192 3350 3988 4059 4246 5614 5893 6561 7246
Size: 158 638 71 187 1368 279 668 685
Cuts at: 7246 8239 8430 33
Size: 993 191 1121
Fragments arranged by size:
1368 1121 1053 993 685 668 638 566
525 519 279 191 187 178 158 124
113 81 71
SfiI GGCCn_nnn'nGGCC
Cuts at: 513 513
Size: 9518
SfoI GGC'GCC
Cuts at: 475 3525 5602 6950 7198 475
Size: 3050 2077 1348 248 2795
Fragments arranged by size:
3050 2795 2077 1348 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9518
SmaI CCC'GGG
Cuts at: 667 1135 2802 3004 3268 6540 667
Size: 468 1667 202 264 3272 3645
Fragments arranged by size:
3645 3272 1667 468 264 202
SmlI C'TyrA_G
Cuts at: 487 854 1650 2740 2983 3978 5053 6673 7588
Size: 367 796 1090 243 995 1075 1620 915
Cuts at: 7588 8080 8342 487
Size: 492 262 1663
Fragments arranged by size:
1663 1620 1090 1075 995 915 796 492
367 262 243
SnaBI TAC'GTA
Cuts at: 1923 1923
Size: 9518
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9518
SphI G_CATG'C
Cuts at: 6571 7071 6571
Size: 500 9018
StuI AGG'CCT
Cuts at: 2035 2098 3274 4139 5799 2035
Size: 63 1176 865 1660 5754
Fragments arranged by size:
5754 1660 1176 865 63
StyI C'CwwG_G
Cuts at: 1262 1340 1620 2117 2320 2600 2828 3854 4047
Size: 78 280 497 203 280 228 1026 193
Cuts at: 4047 4343 5840 6652 7006 1262
Size: 296 1497 812 354 3774
Fragments arranged by size:
3774 1497 1026 812 497 354 296 280
280 228 203 193 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 669 1174 1360 1442 1595 2104 2205 3316 4603
Size: 505 186 82 153 509 101 1111 1287
Cuts at: 4603 4777 4864 5506 7101 7234 7693 7902 669
Size: 174 87 642 1595 133 459 209 2285
Fragments arranged by size:
2285 1595 1287 1111 642 509 505 459
209 186 174 153 133 101 87 82
TatI w'GTAC_w
Cuts at: 746 4412 4637 5228 5963 6386 6398 9033 746
Size: 3666 225 591 735 423 12 2635 1231
Fragments arranged by size:
3666 2635 1231 735 591 423 225 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 868 900 1417 1430 1449 1462 2205 2237 2664
Size: 32 517 13 19 13 743 32 427
Cuts at: 2664 2696 4115 4147 5630 5662 9035 9067 868
Size: 32 1419 32 1483 32 3373 32 1319
Fragments arranged by size:
3373 1483 1419 1319 743 517 427 32
32 32 32 32 32 19 13 13
Tth111I GACn'n_nGTC
Cuts at: 7315 7315
Size: 9518
XbaI T'CTAG_A
Cuts at: 829 6549 829
Size: 5720 3798
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 283 633 726 1296 1301 1365 1604 2407
Size: 169 350 93 570 5 64 239 803
Cuts at: 2407 2550 3792 4350 4779 7013 114
Size: 143 1242 558 429 2234 2619
Fragments arranged by size:
2619 2234 1242 803 570 558 429 350
239 169 143 93 64 5
XhoI C'TCGA_G
Cuts at: 487 854 1650 2983 3978 487
Size: 367 796 1333 995 6027
Fragments arranged by size:
6027 1333 995 796 367
XmaI C'CCGG_G
Cuts at: 665 1133 2800 3002 3266 6538 665
Size: 468 1667 202 264 3272 3645
Fragments arranged by size:
3645 3272 1667 468 264 202
XmnI GAAnn'nnTTC
Cuts at: 4735 5965 4735
Size: 1230 8288
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AscI AvrII BaeI BfrBI BmtI
BsiWI ClaI CspCI EcoRV FspAI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PsrI SgrAI SrfI
SspI SwaI ZraI