Restriction map for
pF1KA0411
(Circular) (Six-base) MAPSORT of: pF1KA0411.seq Check: 8090 from: 1 to: 6338
pF1KA0411 6338 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 2851 3376 2851
Size: 525 5813
Acc65I G'GTAC_C
Cuts at: 3354 3354
Size: 6338
AclI AA'CG_TT
Cuts at: 6178 6178
Size: 6338
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 589 670 1641 3049 4163 4595 4948 5610 589
Size: 81 971 1408 1114 432 353 662 1317
Fragments arranged by size:
1408 1317 1114 971 662 432 353 81
AfeI AGC'GCT
Cuts at: 325 1146 2793 325
Size: 821 1647 3870
Fragments arranged by size:
3870 1647 821
AflIII A'CryG_T
Cuts at: 611 1067 3690 5490 5771 611
Size: 456 2623 1800 281 1178
Fragments arranged by size:
2623 1800 1178 456 281
AgeI A'CCGG_T
Cuts at: 3233 3233
Size: 6338
AhdI GACnn_n'nnGTC
Cuts at: 2052 2541 2892 2052
Size: 489 351 5498
Fragments arranged by size:
5498 489 351
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 727 2965 727
Size: 2238 4100
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 346 378 664 696 346
Size: 32 286 32 5988
Fragments arranged by size:
5988 286 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1506 1765 3643 5081 1506
Size: 259 1878 1438 2763
Fragments arranged by size:
2763 1878 1438 259
ApaI G_GGCC'C
Cuts at: 2677 2677
Size: 6338
ApaLI G'TGCA_C
Cuts at: 1873 5176 1873
Size: 3303 3035
ApoI r'AATT_y
Cuts at: 1432 1698 3342 5691 5947 1432
Size: 266 1644 2349 256 1823
Fragments arranged by size:
2349 1823 1644 266 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6338
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6338
AvaI C'yCGr_G
Cuts at: 289 350 2126 2663 2696 2741 2968 3255 3358
Size: 61 1776 537 33 45 227 287 103
Cuts at: 3358 289
Size: 3269
Fragments arranged by size:
3269 1776 537 287 227 103 61 45
33
AvrII C'CTAG_G
Cuts at: 2880 2880
Size: 6338
BamHI G'GATC_C
Cuts at: 1986 3363 1986
Size: 1377 4961
BanI G'GyrC_C
Cuts at: 1534 2994 3181 3325 3354 3768 4016 4051 1534
Size: 1460 187 144 29 414 248 35 3821
Fragments arranged by size:
3821 1460 414 248 187 144 35 29
BanII G_rGCy'C
Cuts at: 290 566 587 2677 2761 3026 3062 3352 4382
Size: 276 21 2090 84 265 36 290 1030
Cuts at: 4382 290
Size: 2246
Fragments arranged by size:
2246 2090 1030 290 276 265 84 36
21
BbeI G_GCGC'C
Cuts at: 2998 3185 3772 4020 2998
Size: 187 587 248 5316
Fragments arranged by size:
5316 587 248 187
BbsI GAAGACnn'nnnn_
Cuts at: 1074 1724 2927 2938 1074
Size: 650 1203 11 4474
Fragments arranged by size:
4474 1203 650 11
BbvCI CC'TCA_GC
Cuts at: 700 700
Size: 6338
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2266 2300 3082 3116 2266
Size: 34 782 34 5488
Fragments arranged by size:
5488 782 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4236 5292 6338 4236
Size: 1056 1046 4236
Fragments arranged by size:
4236 1056 1046
BclI T'GATC_A
Cuts at: 3858 3858
Size: 6338
BfrBI ATG'CAT
Cuts at: 2159 2159
Size: 6338
BglI GCCn_nnn'nGGC
Cuts at: 937 3175 3179 3216 937
Size: 2238 4 37 4059
Fragments arranged by size:
4059 2238 37 4
BglII A'GATC_T
Cuts at: 3853 5890 3853
Size: 2037 4301
BlpI GC'TnA_GC
Cuts at: 320 1148 3309 3450 320
Size: 828 2161 141 3208
Fragments arranged by size:
3208 2161 828 141
Bme1580I G_kGCm'C
Cuts at: 1877 2677 2840 3328 3963 4056 5180 1877
Size: 800 163 488 635 93 1124 3035
Fragments arranged by size:
3035 1124 800 635 488 163 93
BmrI ACTGGGnnnn_n'
Cuts at: 1956 3550 3631 3966 6110 1956
Size: 1594 81 335 2144 2184
Fragments arranged by size:
2184 2144 1594 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 345 2577 2963 4705 345
Size: 2232 386 1742 1978
Fragments arranged by size:
2232 1978 1742 386
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 238 328 1000 1624 1873 2186 2524 2920
Size: 6 90 672 624 249 313 338 396
Cuts at: 2920 3040 232
Size: 120 3530
Fragments arranged by size:
3530 672 624 396 338 313 249 120
90 6
Bpu10I CC'TnA_GC
Cuts at: 700 2467 700
Size: 1767 4571
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1081 1198 1480 3514 4393 5107 5405 1081
Size: 117 282 2034 879 714 298 2014
Fragments arranged by size:
2034 2014 879 714 298 282 117
BsaI GGTCTCn'nnnn_
Cuts at: 434 689 976 1351 6042 434
Size: 255 287 375 4691 730
Fragments arranged by size:
4691 730 375 287 255
BsaAI yAC'GTr
Cuts at: 3691 3691
Size: 6338
BsaBI GATnn'nnATC
Cuts at: 6 3852 5889 6
Size: 3846 2037 455
Fragments arranged by size:
3846 2037 455
BsaHI Gr'CG_yC
Cuts at: 2631 2995 3182 3280 3769 4017 4719 6215 2631
Size: 364 187 98 489 248 702 1496 2754
Fragments arranged by size:
2754 1496 702 489 364 248 187 98
BsaWI w'CCGG_w
Cuts at: 950 3233 3527 4048 4726 5137 5284 5558 950
Size: 2283 294 521 678 411 147 274 1730
Fragments arranged by size:
2283 1730 678 521 411 294 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 346 376 664 694 1942 1972 2362 2392 346
Size: 30 288 30 1248 30 390 30 4292
Fragments arranged by size:
4292 1248 390 288 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 321 373 574 685 1243 1404 3117 321
Size: 52 201 111 558 161 1713 3542
Fragments arranged by size:
3542 1713 558 201 161 111 52
BseYI C'CCAG_C
Cuts at: 734 1860 2119 2677 2713 3185 3763 4521 5186
Size: 1126 259 558 36 472 578 758 665
Cuts at: 5186 5594 734
Size: 408 1478
Fragments arranged by size:
1478 1126 758 665 578 558 472 408
259 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2935 2935
Size: 6338
BsiEI CG_ry'CG
Cuts at: 104 1327 3220 3275 3880 3926 5156 104
Size: 1223 1893 55 605 46 1230 1286
Fragments arranged by size:
1893 1286 1230 1223 605 55 46
BsiHKAI G_wGCw'C
Cuts at: 290 359 566 587 1877 2558 2944 3352 4130
Size: 69 207 21 1290 681 386 408 778
Cuts at: 4130 4320 5180 290
Size: 190 860 1448
Fragments arranged by size:
1448 1290 860 778 681 408 386 207
190 69 21
BsmI GAATG_Cn'
Cuts at: 1023 1534 1023
Size: 511 5827
Bsp1286I G_dGCh'C
Cuts at: 290 359 566 587 1877 2558 2677 2761 2840
Size: 69 207 21 1290 681 119 84 79
Cuts at: 2840 2944 3026 3062 3328 3352 3963 4056 4130
Size: 104 82 36 266 24 611 93 74
Cuts at: 4130 4320 4382 5180 290
Size: 190 62 798 1448
Fragments arranged by size:
1448 1290 798 681 611 266 207 190
119 104 93 84 82 79 74 69
62 36 24 21
BspEI T'CCGG_A
Cuts at: 950 950
Size: 6338
BspHI T'CATG_A
Cuts at: 467 596 1925 2103 4770 467
Size: 129 1329 178 2667 2035
Fragments arranged by size:
2667 2035 1329 178 129
BspMI ACCTGCnnnn'nnnn_
Cuts at: 719 2863 3388 3602 3904 4285 719
Size: 2144 525 214 302 381 2772
Fragments arranged by size:
2772 2144 525 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4630 4684 6168 6333 50
Size: 4580 54 1484 165 55
Fragments arranged by size:
4580 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 146 1379 2503 4250 146
Size: 1233 1124 1747 2234
Fragments arranged by size:
2234 1747 1233 1124
BsrFI r'CCGG_y
Cuts at: 728 2387 3219 3233 4336 4517 728
Size: 1659 832 14 1103 181 2549
Fragments arranged by size:
2549 1659 1103 832 181 14
BsrGI T'GTAC_A
Cuts at: 2433 2433
Size: 6338
BssSI C'ACGA_G
Cuts at: 556 721 2953 4609 5317 556
Size: 165 2232 1656 708 1577
Fragments arranged by size:
2232 1656 1577 708 165
BstAPI GCAn_nnn'nTGC
Cuts at: 2958 3715 2958
Size: 757 5581
BstBI TT'CG_AA
Cuts at: 4699 4699
Size: 6338
BstEII G'GTnAC_C
Cuts at: 573 573
Size: 6338
BstXI CCAn_nnnn'nTGG
Cuts at: 2233 2233
Size: 6338
BstYI r'GATC_y
Cuts at: 1 176 1652 1669 1880 1986 3363 3830 3853
Size: 175 1476 17 211 106 1377 467 23
Cuts at: 3853 4188 4434 4740 4752 4838 5890 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1476 1377 1052 467 449 335 306 246
211 175 106 86 23 17 12
Bsu36I CC'TnA_GG
Cuts at: 1814 1814
Size: 6338
BtgI C'CryG_G
Cuts at: 2549 3329 2549
Size: 780 5558
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2270 4288 4469 4632 2270
Size: 2018 181 163 3976
Fragments arranged by size:
3976 2018 181 163
BtsI GCAGTG_nn'
Cuts at: 1028 2502 3581 1028
Size: 1474 1079 3785
Fragments arranged by size:
3785 1474 1079
ClaI AT'CG_AT
Cuts at: 905 2213 905
Size: 1308 5030
DraI TTT'AAA
Cuts at: 1437 1775 3337 1437
Size: 338 1562 4438
Fragments arranged by size:
4438 1562 338
DrdI GACnn_nn'nnGTC
Cuts at: 1297 4044 5388 1297
Size: 2747 1344 2247
Fragments arranged by size:
2747 2247 1344
EaeI y'GGCC_r
Cuts at: 1205 2000 3217 3272 3923 4097 4488 4515 5860
Size: 795 1217 55 651 174 391 27 1345
Cuts at: 5860 1205
Size: 1683
Fragments arranged by size:
1683 1345 1217 795 651 391 174 55
27
EagI C'GGCC_G
Cuts at: 3217 3272 3923 3217
Size: 55 651 5632
Fragments arranged by size:
5632 651 55
EarI CTCTTCn'nnn_
Cuts at: 526 772 1942 2529 4361 4571 526
Size: 246 1170 587 1832 210 2293
Fragments arranged by size:
2293 1832 1170 587 246 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 256 475 3156 3181 4546 5288 5434 6148 256
Size: 219 2681 25 1365 742 146 714 446
Fragments arranged by size:
2681 1365 742 714 446 219 146 25
EcoICRI GAG'CTC
Cuts at: 288 564 585 3350 288
Size: 276 21 2765 3276
Fragments arranged by size:
3276 2765 276 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 238 328 589 670 1000 1624 1641 1873
Size: 6 90 261 81 330 624 17 232
Cuts at: 1873 2186 2524 2920 3040 3049 4163 4595 4948
Size: 313 338 396 120 9 1114 432 353
Cuts at: 4948 5610 232
Size: 662 960
Fragments arranged by size:
1114 960 662 624 432 396 353 338
330 313 261 232 120 90 81 17
9 6
EcoO109I rG'GnC_Cy
Cuts at: 2046 2140 2339 2667 2674 2910 3043 3477 2046
Size: 94 199 328 7 236 133 434 4907
Fragments arranged by size:
4907 434 328 236 199 133 94 7
EcoRI G'AATT_C
Cuts at: 3342 3342
Size: 6338
EcoRV GAT'ATC
Cuts at: 1752 1838 1752
Size: 86 6252
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1741 4357 1741
Size: 2616 3722
FspI TGC'GCA
Cuts at: 4119 4119
Size: 6338
HaeII r_GCGC'y
Cuts at: 327 741 1148 2795 2827 2998 3185 3772 4020
Size: 414 407 1647 32 171 187 587 248
Cuts at: 4020 5250 6021 327
Size: 1230 771 644
Fragments arranged by size:
1647 1230 771 644 587 414 407 248
187 171 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 205 237 1822 1854 2038 2070 2440 2472 2506
Size: 32 1585 32 184 32 370 32 34
Cuts at: 2506 2527 2538 2559 2606 2638 2878 2910 4180
Size: 21 11 21 47 32 240 32 1270
Cuts at: 4180 4210 4212 4242 205
Size: 30 2 30 2301
Fragments arranged by size:
2301 1585 1270 370 240 184 47 34
32 32 32 32 32 32 30 30
21 21 11 2
HincII GTy'rAC
Cuts at: 2223 2852 3377 2223
Size: 629 525 5184
Fragments arranged by size:
5184 629 525
KasI G'GCGC_C
Cuts at: 2994 3181 3768 4016 2994
Size: 187 587 248 5316
Fragments arranged by size:
5316 587 248 187
KpnI G_GTAC'C
Cuts at: 3358 3358
Size: 6338
MluI A'CGCG_T
Cuts at: 5771 5771
Size: 6338
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1338 1908 2483 5097 5281 1338
Size: 570 575 2614 184 2395
Fragments arranged by size:
2614 2395 575 570 184
MscI TGG'CCA
Cuts at: 1207 2002 4099 1207
Size: 795 2097 3446
Fragments arranged by size:
3446 2097 795
MslI CAynn'nnrTG
Cuts at: 466 595 1060 1066 2072 2231 3136 4327 4454
Size: 129 465 6 1006 159 905 1191 127
Cuts at: 4454 466
Size: 2350
Fragments arranged by size:
2350 1191 1006 905 465 159 129 127
6
MspA1I CmG'CkG
Cuts at: 189 240 967 1191 3313 3449 3763 4123 4907
Size: 51 727 224 2122 136 314 360 784
Cuts at: 4907 5152 189
Size: 245 1375
Fragments arranged by size:
2122 1375 784 727 360 314 245 224
136 51
NaeI GCC'GGC
Cuts at: 2389 4519 2389
Size: 2130 4208
NarI GG'CG_CC
Cuts at: 2995 3182 3769 4017 2995
Size: 187 587 248 5316
Fragments arranged by size:
5316 587 248 187
NcoI C'CATG_G
Cuts at: 3329 3329
Size: 6338
NgoMIV G'CCGG_C
Cuts at: 2387 4517 2387
Size: 2130 4208
NruI TCG'CGA
Cuts at: 231 231
Size: 6338
NsiI A_TGCA'T
Cuts at: 2161 2161
Size: 6338
NspI r_CATG'y
Cuts at: 615 1071 1278 3391 3891 5494 615
Size: 456 207 2113 500 1603 1459
Fragments arranged by size:
2113 1603 1459 500 456 207
PasI CC'CwG_GG
Cuts at: 1506 1506
Size: 6338
PciI A'CATG_T
Cuts at: 611 1067 5490 611
Size: 456 4423 1459
Fragments arranged by size:
4423 1459 456
PfoI T'CCnGG_A
Cuts at: 254 254
Size: 6338
PmeI GTTT'AAAC
Cuts at: 3337 3337
Size: 6338
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 346 378 664 696 3280 3312 4784 4816 346
Size: 32 286 32 2584 32 1472 32 1868
Fragments arranged by size:
2584 1868 1472 286 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2046 2140 2046
Size: 94 6244
PspOMI G'GGCC_C
Cuts at: 2673 2673
Size: 6338
PspXI vC'TCGA_Gb
Cuts at: 289 289
Size: 6338
PstI C_TGCA'G
Cuts at: 239 551 1196 1478 3385 4070 239
Size: 312 645 282 1907 685 2507
Fragments arranged by size:
2507 1907 685 645 312 282
PvuI CG_AT'CG
Cuts at: 104 1327 104
Size: 1223 5115
PvuII CAG'CTG
Cuts at: 189 240 967 1191 3763 4123 189
Size: 51 727 224 2572 360 2404
Fragments arranged by size:
2572 2404 727 360 224 51
RsrII CG'GwC_CG
Cuts at: 4533 4533
Size: 6338
SacI G_AGCT'C
Cuts at: 290 566 587 3352 290
Size: 276 21 2765 3276
Fragments arranged by size:
3276 2765 276 21
SalI G'TCGA_C
Cuts at: 2850 3375 2850
Size: 525 5813
SapI GCTCTTCn'nnn_
Cuts at: 4361 4571 4361
Size: 210 6128
SbfI CC_TGCA'GG
Cuts at: 3385 3385
Size: 6338
ScaI AGT'ACT
Cuts at: 295 793 5855 295
Size: 498 5062 778
Fragments arranged by size:
5062 778 498
SfcI C'TryA_G
Cuts at: 33 235 547 1192 1474 3381 4066 5034 5225
Size: 202 312 645 282 1907 685 968 191
Cuts at: 5225 33
Size: 1146
Fragments arranged by size:
1907 1146 968 685 645 312 282 202
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3216 3216
Size: 6338
SfoI GGC'GCC
Cuts at: 2996 3183 3770 4018 2996
Size: 187 587 248 5316
Fragments arranged by size:
5316 587 248 187
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6338
SmaI CCC'GGG
Cuts at: 352 2128 2665 2698 2743 3360 352
Size: 1776 537 33 45 617 3330
Fragments arranged by size:
3330 1776 617 537 45 33
SmlI C'TyrA_G
Cuts at: 289 1096 1213 1495 2968 3493 4408 5122 5384
Size: 807 117 282 1473 525 915 714 262
Cuts at: 5384 289
Size: 1243
Fragments arranged by size:
1473 1243 915 807 714 525 282 262
117
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6338
SphI G_CATG'C
Cuts at: 3391 3891 3391
Size: 500 5838
SspI AAT'ATT
Cuts at: 2179 2179
Size: 6338
StuI AGG'CCT
Cuts at: 2521 2521
Size: 6338
StyI C'CwwG_G
Cuts at: 2880 3329 3472 3826 2880
Size: 449 143 354 5392
Fragments arranged by size:
5392 449 354 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3921 4054 4513 4722 3921
Size: 133 459 209 5537
Fragments arranged by size:
5537 459 209 133
TatI w'GTAC_w
Cuts at: 293 791 2423 2433 5853 293
Size: 498 1632 10 3420 778
Fragments arranged by size:
3420 1632 778 498 10
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1661 1693 2362 2394 5855 5887 1661
Size: 32 669 32 3461 32 2112
Fragments arranged by size:
3461 2112 669 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1332 4135 1332
Size: 2803 3535
XbaI T'CTAG_A
Cuts at: 3369 3369
Size: 6338
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3833 3833
Size: 6338
XhoI C'TCGA_G
Cuts at: 289 2968 289
Size: 2679 3659
XmaI C'CCGG_G
Cuts at: 350 2126 2663 2696 2741 3358 350
Size: 1776 537 33 45 617 3330
Fragments arranged by size:
3330 1776 617 537 45 33
XmnI GAAnn'nnTTC
Cuts at: 679 1823 3295 679
Size: 1144 1472 3722
Fragments arranged by size:
3722 1472 1144
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI
NsiI NspI PasI PciI PfoI PmeI PpiI PpuMI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AleI AscI BaeI BmgBI BmtI
BsiWI BsmBI BssHII BstZ17I CspCI DraIII EcoNI FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI PacI
PflMI PmlI PshAI PsiI PsrI SacII SanDI SexAI
SgrAI SnaBI SrfI SwaI ZraI