Restriction map for
pF1KA0418
(Circular) (Six-base) MAPSORT of: pF1KA0418.seq Check: 4373 from: 1 to: 5933
pF1KA0418 5933 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2121 2439 2121
Size: 318 5615
AccI GT'mk_AC
Cuts at: 2971 2971
Size: 5933
Acc65I G'GTAC_C
Cuts at: 2949 2949
Size: 5933
AclI AA'CG_TT
Cuts at: 2927 5773 2927
Size: 2846 3087
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 1337 1702 3758 4190 4937 5205 508
Size: 829 365 2056 432 747 268 1236
Fragments arranged by size:
2056 1236 829 747 432 365 268
AflIII A'CryG_T
Cuts at: 3285 4389 5366 3285
Size: 1104 977 3852
Fragments arranged by size:
3852 1104 977
AgeI A'CCGG_T
Cuts at: 4321 4321
Size: 5933
AhdI GACnn_n'nnGTC
Cuts at: 1425 1772 1425
Size: 347 5586
AlwNI CAG_nnn'CTG
Cuts at: 1234 2136 2868 3238 4805 1234
Size: 902 732 370 1567 2362
Fragments arranged by size:
2362 1567 902 732 370
ApaI G_GGCC'C
Cuts at: 780 1020 1141 1332 2196 780
Size: 240 121 191 864 4517
Fragments arranged by size:
4517 864 240 191 121
ApaLI G'TGCA_C
Cuts at: 4703 4703
Size: 5933
ApoI r'AATT_y
Cuts at: 901 2937 5286 5542 901
Size: 2036 2349 256 1292
Fragments arranged by size:
2349 2036 1292 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5933
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5933
AvaI C'yCGr_G
Cuts at: 290 1778 2389 2953 290
Size: 1488 611 564 3270
Fragments arranged by size:
3270 1488 611 564
BamHI G'GATC_C
Cuts at: 2958 2958
Size: 5933
BanI G'GyrC_C
Cuts at: 471 1092 1865 2898 2949 3363 3611 3646 471
Size: 621 773 1033 51 414 248 35 2758
Fragments arranged by size:
2758 1033 773 621 414 248 51 35
BanII G_rGCy'C
Cuts at: 745 780 866 1020 1141 1253 1332 1904 1912
Size: 35 86 154 121 112 79 572 8
Cuts at: 1912 2196 2947 3977 745
Size: 284 751 1030 2701
Fragments arranged by size:
2701 1030 751 572 284 154 121 112
86 79 35 8
BbeI G_GCGC'C
Cuts at: 475 1869 3367 3615 475
Size: 1394 1498 248 2793
Fragments arranged by size:
2793 1498 1394 248
BbsI GAAGACnn'nnnn_
Cuts at: 1343 1343
Size: 5933
BbvCI CC'TCA_GC
Cuts at: 770 1587 770
Size: 817 5116
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2617 2651 2617
Size: 34 5899
BciVI GTATCCnnnnn_n'
Cuts at: 3831 4592 5933 3831
Size: 761 1341 3831
Fragments arranged by size:
3831 1341 761
BclI T'GATC_A
Cuts at: 3453 3453
Size: 5933
BglI GCCn_nnn'nGGC
Cuts at: 1299 1954 2558 2608 2632 1299
Size: 655 604 50 24 4600
Fragments arranged by size:
4600 655 604 50 24
BglII A'GATC_T
Cuts at: 3448 5485 3448
Size: 2037 3896
BlpI GC'TnA_GC
Cuts at: 166 1240 3045 166
Size: 1074 1805 3054
Fragments arranged by size:
3054 1805 1074
Bme1580I G_kGCm'C
Cuts at: 780 1020 1097 1141 1332 2196 2267 3558 3651
Size: 240 77 44 191 864 71 1291 93
Cuts at: 3651 4707 780
Size: 1056 2006
Fragments arranged by size:
2006 1291 1056 864 240 191 93 77
71 44
BmrI ACTGGGnnnn_n'
Cuts at: 532 1955 2460 3145 3226 3561 5705 532
Size: 1423 505 685 81 335 2144 760
Fragments arranged by size:
2144 1423 760 685 505 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4300 4300
Size: 5933
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 292 344 711 731 2158 2203 2326 2402
Size: 135 52 367 20 1427 45 123 76
Cuts at: 2402 2851 157
Size: 449 3239
Fragments arranged by size:
3239 1427 449 367 135 123 76 52
45 20
Bpu10I CC'TnA_GC
Cuts at: 770 1057 1235 1587 770
Size: 287 178 352 5116
Fragments arranged by size:
5116 352 287 178
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1165 1525 1888 2935 3109 3988 4480 4778 1165
Size: 360 363 1047 174 879 492 298 2320
Fragments arranged by size:
2320 1047 879 492 363 360 298 174
BsaI GGTCTCn'nnnn_
Cuts at: 1372 1869 5637 1372
Size: 497 3768 1668
Fragments arranged by size:
3768 1668 497
BsaAI yAC'GTr
Cuts at: 126 990 3286 126
Size: 864 2296 2773
Fragments arranged by size:
2773 2296 864
BsaBI GATnn'nnATC
Cuts at: 6 1849 3447 5484 6
Size: 1843 1598 2037 455
Fragments arranged by size:
2037 1843 1598 455
BsaHI Gr'CG_yC
Cuts at: 398 472 592 1866 2561 3364 3612 4314 5810
Size: 74 120 1274 695 803 248 702 1496
Cuts at: 5810 398
Size: 521
Fragments arranged by size:
1496 1274 803 702 695 521 248 120
74
BsaWI w'CCGG_w
Cuts at: 416 3122 3643 4321 4595 4742 5153 416
Size: 2706 521 678 274 147 411 1196
Fragments arranged by size:
2706 1196 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 795 825 1183 1213 1441 1471 2140 2170 2800
Size: 30 358 30 228 30 669 30 630
Cuts at: 2800 2830 795
Size: 30 3898
Fragments arranged by size:
3898 669 630 358 228 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 256 346 1147 1204 1261 1390 1431 1461 1575
Size: 90 801 57 57 129 41 30 114
Cuts at: 1575 1623 1626 1635 1638 1641 2565 2803 256
Size: 48 3 9 3 3 924 238 3386
Fragments arranged by size:
3386 924 801 238 129 114 90 57
57 48 41 30 9 3 3 3
BseYI C'CCAG_C
Cuts at: 251 467 1013 1111 1289 1759 2004 2189 2672
Size: 216 546 98 178 470 245 185 483
Cuts at: 2672 2894 3358 4116 4693 5189 251
Size: 222 464 758 577 496 995
Fragments arranged by size:
995 758 577 546 496 483 470 464
245 222 216 185 178 98
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 1012 2149 370
Size: 642 1137 4154
Fragments arranged by size:
4154 1137 642
BsiEI CG_ry'CG
Cuts at: 104 3475 3521 4729 104
Size: 3371 46 1208 1308
Fragments arranged by size:
3371 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 235 2947 3725 3915 4707 235
Size: 2712 778 190 792 1461
Fragments arranged by size:
2712 1461 792 778 190
BsmI GAATG_Cn'
Cuts at: 1213 1560 1213
Size: 347 5586
BsmBI CGTCTCn'nnnn_
Cuts at: 2766 2766
Size: 5933
Bsp1286I G_dGCh'C
Cuts at: 235 745 780 866 1020 1097 1141 1253 1332
Size: 510 35 86 154 77 44 112 79
Cuts at: 1332 1904 1912 2196 2267 2947 3558 3651 3725
Size: 572 8 284 71 680 611 93 74
Cuts at: 3725 3915 3977 4707 235
Size: 190 62 730 1461
Fragments arranged by size:
1461 730 680 611 572 510 284 190
154 112 93 86 79 77 74 71
62 44 35 8
BspEI T'CCGG_A
Cuts at: 416 5153 416
Size: 4737 1196
BspHI T'CATG_A
Cuts at: 5109 5109
Size: 5933
BspMI ACCTGCnnnn'nnnn_
Cuts at: 515 584 2121 2439 2983 3197 3499 3880 515
Size: 69 1537 318 544 214 302 381 2568
Fragments arranged by size:
2568 1537 544 381 318 302 214 69
BsrBI CCG'CTC
Cuts at: 50 215 1691 2153 4225 4279 5763 5928 50
Size: 165 1476 462 2072 54 1484 165 55
Fragments arranged by size:
2072 1484 1476 462 165 165 55 54
BsrDI GCAATG_nn'
Cuts at: 644 687 893 3845 644
Size: 43 206 2952 2732
Fragments arranged by size:
2952 2732 206 43
BsrFI r'CCGG_y
Cuts at: 532 1298 1308 3931 4112 4321 532
Size: 766 10 2623 181 209 2144
Fragments arranged by size:
2623 2144 766 209 181 10
BssSI C'ACGA_G
Cuts at: 4204 4562 4204
Size: 358 5575
BstAPI GCAn_nnn'nTGC
Cuts at: 3310 3310
Size: 5933
BstBI TT'CG_AA
Cuts at: 4294 4294
Size: 5933
BstXI CCAn_nnnn'nTGG
Cuts at: 114 270 1894 114
Size: 156 1624 4153
Fragments arranged by size:
4153 1624 156
BstYI r'GATC_y
Cuts at: 1 2864 2958 3425 3448 3783 4029 5041 5127
Size: 2863 94 467 23 335 246 1012 86
Cuts at: 5127 5139 5485 1
Size: 12 346 449
Fragments arranged by size:
2863 1012 467 449 346 335 246 94
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1269 2409 1269
Size: 1140 4793
BtgI C'CryG_G
Cuts at: 701 1171 1253 1713 1893 2114 2819 701
Size: 470 82 460 180 221 705 3815
Fragments arranged by size:
3815 705 470 460 221 180 82
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3883 4064 4227 3883
Size: 181 163 5589
Fragments arranged by size:
5589 181 163
BtsI GCAGTG_nn'
Cuts at: 702 2585 3176 702
Size: 1883 591 3459
Fragments arranged by size:
3459 1883 591
ClaI AT'CG_AT
Cuts at: 1034 1034
Size: 5933
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1653 1688 1653
Size: 35 5898
DraI TTT'AAA
Cuts at: 2932 2932
Size: 5933
DrdI GACnn_nn'nnGTC
Cuts at: 721 3639 4497 721
Size: 2918 858 2157
Fragments arranged by size:
2918 2157 858
EaeI y'GGCC_r
Cuts at: 530 755 1405 2540 2624 3518 3692 4083 4110
Size: 225 650 1135 84 894 174 391 27
Cuts at: 4110 5455 530
Size: 1345 1008
Fragments arranged by size:
1345 1135 1008 894 650 391 225 174
84 27
EagI C'GGCC_G
Cuts at: 3518 3518
Size: 5933
EarI CTCTTCn'nnn_
Cuts at: 323 1320 1367 1644 1659 3956 4166 323
Size: 997 47 277 15 2297 210 2090
Fragments arranged by size:
2297 2090 997 277 210 47 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4141 4451 4597 5743 4141
Size: 310 146 1146 4331
Fragments arranged by size:
4331 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2945 2945
Size: 5933
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 292 344 508 711 731 1337 1702 2158
Size: 135 52 164 203 20 606 365 456
Cuts at: 2158 2203 2326 2402 2851 3758 4190 4937 5205
Size: 45 123 76 449 907 432 747 268
Cuts at: 5205 157
Size: 885
Fragments arranged by size:
907 885 747 606 456 449 432 365
268 203 164 135 123 76 52 45
20
EcoNI CCTnn'n_nnAGG
Cuts at: 490 1372 490
Size: 882 5051
EcoO109I rG'GnC_Cy
Cuts at: 255 639 1017 1137 1138 2193 2462 3072 255
Size: 384 378 120 1 1055 269 610 3116
Fragments arranged by size:
3116 1055 610 384 378 269 120 1
EcoRI G'AATT_C
Cuts at: 901 2937 901
Size: 2036 3897
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3952 3952
Size: 5933
FseI GG_CCGG'CC
Cuts at: 536 536
Size: 5933
FspI TGC'GCA
Cuts at: 3714 3714
Size: 5933
HaeII r_GCGC'y
Cuts at: 475 1869 2078 3367 3615 4637 5616 475
Size: 1394 209 1289 248 1022 979 792
Fragments arranged by size:
1394 1289 1022 979 792 248 209
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 753 785 795 827 955 987 1252 1284 1411
Size: 32 10 32 128 32 265 32 127
Cuts at: 1411 1441 1443 1473 1758 1790 2800 2832 3775
Size: 30 2 30 285 32 1010 32 943
Cuts at: 3775 3805 3807 3837 753
Size: 30 2 30 2849
Fragments arranged by size:
2849 1010 943 285 265 128 127 32
32 32 32 32 32 30 30 30
30 10 2 2
HincII GTy'rAC
Cuts at: 2346 2972 2346
Size: 626 5307
KasI G'GCGC_C
Cuts at: 471 1865 3363 3611 471
Size: 1394 1498 248 2793
Fragments arranged by size:
2793 1498 1394 248
KpnI G_GTAC'C
Cuts at: 2953 2953
Size: 5933
MluI A'CGCG_T
Cuts at: 5366 5366
Size: 5933
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 161 326 867 2012 2589 2930 4604 4788 161
Size: 165 541 1145 577 341 1674 184 1306
Fragments arranged by size:
1674 1306 1145 577 541 341 184 165
MscI TGG'CCA
Cuts at: 757 3694 757
Size: 2937 2996
MslI CAynn'nnrTG
Cuts at: 2689 3922 4049 2689
Size: 1233 127 4573
Fragments arranged by size:
4573 1233 127
MspA1I CmG'CkG
Cuts at: 751 774 1173 1326 1715 1872 2116 2422 2599
Size: 23 399 153 389 157 244 306 177
Cuts at: 2599 3044 3358 3718 4731 4976 751
Size: 445 314 360 1013 245 1708
Fragments arranged by size:
1708 1013 445 399 389 360 314 306
245 244 177 157 153 23
NaeI GCC'GGC
Cuts at: 534 1310 4114 534
Size: 776 2804 2353
Fragments arranged by size:
2804 2353 776
NarI GG'CG_CC
Cuts at: 472 1866 3364 3612 472
Size: 1394 1498 248 2793
Fragments arranged by size:
2793 1498 1394 248
NcoI C'CATG_G
Cuts at: 2819 2819
Size: 5933
NdeI CA'TA_TG
Cuts at: 1410 1410
Size: 5933
NgoMIV G'CCGG_C
Cuts at: 532 1308 4112 532
Size: 776 2804 2353
Fragments arranged by size:
2804 2353 776
NspI r_CATG'y
Cuts at: 2072 2986 3486 4393 2072
Size: 914 500 907 3612
Fragments arranged by size:
3612 914 907 500
PasI CC'CwG_GG
Cuts at: 738 738
Size: 5933
PciI A'CATG_T
Cuts at: 4389 4389
Size: 5933
PflMI CCAn_nnn'nTGG
Cuts at: 2208 2868 2208
Size: 660 5273
PfoI T'CCnGG_A
Cuts at: 113 2090 2804 2867 113
Size: 1977 714 63 3179
Fragments arranged by size:
3179 1977 714 63
PmeI GTTT'AAAC
Cuts at: 2932 2932
Size: 5933
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1570 1602 5072 5104 1570
Size: 32 3470 32 2399
Fragments arranged by size:
3470 2399 32 32
PpuMI rG'GwC_Cy
Cuts at: 255 255
Size: 5933
PspOMI G'GGCC_C
Cuts at: 776 1016 1137 1328 2192 776
Size: 240 121 191 864 4517
Fragments arranged by size:
4517 864 240 191 121
PstI C_TGCA'G
Cuts at: 1325 2980 3665 1325
Size: 1655 685 3593
Fragments arranged by size:
3593 1655 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5933
PvuII CAG'CTG
Cuts at: 751 1872 3358 3718 751
Size: 1121 1486 360 2966
Fragments arranged by size:
2966 1486 1121 360
RsrII CG'GwC_CG
Cuts at: 1794 4128 1794
Size: 2334 3599
SacI G_AGCT'C
Cuts at: 2947 2947
Size: 5933
SacII CC_GC'GG
Cuts at: 1174 1716 2117 1174
Size: 542 401 4990
Fragments arranged by size:
4990 542 401
SalI G'TCGA_C
Cuts at: 2970 2970
Size: 5933
SanDI GG'GwC_CC
Cuts at: 255 255
Size: 5933
SapI GCTCTTCn'nnn_
Cuts at: 3956 4166 3956
Size: 210 5723
SbfI CC_TGCA'GG
Cuts at: 2980 2980
Size: 5933
ScaI AGT'ACT
Cuts at: 886 5450 886
Size: 4564 1369
SfcI C'TryA_G
Cuts at: 33 1321 2976 3661 4654 4845 33
Size: 1288 1655 685 993 191 1121
Fragments arranged by size:
1655 1288 1121 993 685 191
SfoI GGC'GCC
Cuts at: 473 1867 3365 3613 473
Size: 1394 1498 248 2793
Fragments arranged by size:
2793 1498 1394 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5933
SmaI CCC'GGG
Cuts at: 1780 2391 2955 1780
Size: 611 564 4758
Fragments arranged by size:
4758 611 564
SmlI C'TyrA_G
Cuts at: 1180 1540 1903 2914 3088 4003 4495 4757 1180
Size: 360 363 1011 174 915 492 262 2356
Fragments arranged by size:
2356 1011 915 492 363 360 262 174
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5933
SphI G_CATG'C
Cuts at: 2986 3486 2986
Size: 500 5433
StyI C'CwwG_G
Cuts at: 497 1088 1121 1724 2270 2492 2612 2819 3067
Size: 591 33 603 546 222 120 207 248
Cuts at: 3067 3421 497
Size: 354 3009
Fragments arranged by size:
3009 603 591 546 354 248 222 207
120 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1333 1782 2505 2886 3516 3649 4108 4317 1333
Size: 449 723 381 630 133 459 209 2949
Fragments arranged by size:
2949 723 630 459 449 381 209 133
TatI w'GTAC_w
Cuts at: 884 5448 884
Size: 4564 1369
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 421 453 2140 2172 5450 5482 421
Size: 32 1687 32 3278 32 872
Fragments arranged by size:
3278 1687 872 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3730 3730
Size: 5933
XbaI T'CTAG_A
Cuts at: 2964 2964
Size: 5933
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2619 2826 2869 3428 2619
Size: 207 43 559 5124
Fragments arranged by size:
5124 559 207 43
XmaI C'CCGG_G
Cuts at: 1778 2389 2953 1778
Size: 611 564 4758
Fragments arranged by size:
4758 611 564
XmnI GAAnn'nnTTC
Cuts at: 2884 2884
Size: 5933
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI AscI AvrII
BaeI BfrBI BmgBI BmtI BsiWI BsrGI BssHII BstEII
BstZ17I DraIII EcoRV FspAI HindIII HpaI MfeI NheI
NotI NruI NsiI PacI PmlI PshAI PsiI PspXI
PsrI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI