Restriction map for
pF1KA0419
(Circular) (Six-base) MAPSORT of: pF1KA0419.seq Check: 2795 from: 1 to: 6086
pF1KA0419 6086 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 2544 3000 3124 2544
Size: 456 124 5506
Fragments arranged by size:
5506 456 124
Acc65I G'GTAC_C
Cuts at: 3102 3102
Size: 6086
AclI AA'CG_TT
Cuts at: 5926 5926
Size: 6086
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 665 775 1469 1484 2587 2656 2938 3911 4343
Size: 110 694 15 1103 69 282 973 432
Cuts at: 4343 5090 5358 665
Size: 747 268 1393
Fragments arranged by size:
1393 1103 973 747 694 432 282 268
110 69 15
AflIII A'CryG_T
Cuts at: 3438 4542 5519 3438
Size: 1104 977 4005
Fragments arranged by size:
4005 1104 977
AgeI A'CCGG_T
Cuts at: 4474 4474
Size: 6086
AleI CACnn'nnGTG
Cuts at: 1366 1366
Size: 6086
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1063 1063
Size: 6086
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1083 1115 1083
Size: 32 6054
AlwNI CAG_nnn'CTG
Cuts at: 798 1598 3391 4958 798
Size: 800 1793 1567 1926
Fragments arranged by size:
1926 1793 1567 800
ApaLI G'TGCA_C
Cuts at: 4856 4856
Size: 6086
ApoI r'AATT_y
Cuts at: 2319 2500 2602 2914 3090 5439 5695 2319
Size: 181 102 312 176 2349 256 2710
Fragments arranged by size:
2710 2349 312 256 181 176 102
AseI AT'TA_AT
Cuts at: 20 20
Size: 6086
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6086
AvaI C'yCGr_G
Cuts at: 3106 3106
Size: 6086
BamHI G'GATC_C
Cuts at: 3111 3111
Size: 6086
BanI G'GyrC_C
Cuts at: 3102 3516 3764 3799 3102
Size: 414 248 35 5389
Fragments arranged by size:
5389 414 248 35
BanII G_rGCy'C
Cuts at: 3100 4130 3100
Size: 1030 5056
BbeI G_GCGC'C
Cuts at: 3520 3768 3520
Size: 248 5838
BbsI GAAGACnn'nnnn_
Cuts at: 781 1245 3069 781
Size: 464 1824 3798
Fragments arranged by size:
3798 1824 464
BciVI GTATCCnnnnn_n'
Cuts at: 3984 4745 6086 3984
Size: 761 1341 3984
Fragments arranged by size:
3984 1341 761
BclI T'GATC_A
Cuts at: 408 3606 408
Size: 3198 2888
BglII A'GATC_T
Cuts at: 3601 5638 3601
Size: 2037 4049
BlpI GC'TnA_GC
Cuts at: 2123 3198 2123
Size: 1075 5011
Bme1580I G_kGCm'C
Cuts at: 1373 3711 3804 4860 1373
Size: 2338 93 1056 2599
Fragments arranged by size:
2599 2338 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 3298 3379 3714 5858 3298
Size: 81 335 2144 3526
Fragments arranged by size:
3526 2144 335 81
BmtI G_CTAG'C
Cuts at: 1064 1064
Size: 6086
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4453 4453
Size: 6086
Bpu10I CC'TnA_GC
Cuts at: 2800 2800
Size: 6086
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1013 1666 2279 2456 2642 3262 4141 4633 4931
Size: 653 613 177 186 620 879 492 298
Cuts at: 4931 1013
Size: 2168
Fragments arranged by size:
2168 879 653 620 613 492 298 186
177
BsaI GGTCTCn'nnnn_
Cuts at: 2474 5790 2474
Size: 3316 2770
BsaAI yAC'GTr
Cuts at: 3439 3439
Size: 6086
BsaBI GATnn'nnATC
Cuts at: 6 407 3600 5637 6
Size: 401 3193 2037 455
Fragments arranged by size:
3193 2037 455 401
BsaHI Gr'CG_yC
Cuts at: 3517 3765 4467 5963 3517
Size: 248 702 1496 3640
Fragments arranged by size:
3640 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3275 3796 4474 4748 4895 5306 3275
Size: 521 678 274 147 411 4055
Fragments arranged by size:
4055 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2164 2194 2164
Size: 30 6056
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 127 2047 127
Size: 1920 4166
BseYI C'CCAG_C
Cuts at: 535 2099 3511 4269 4846 5342 535
Size: 1564 1412 758 577 496 1279
Fragments arranged by size:
1564 1412 1279 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 3628 3674 4882 104
Size: 3524 46 1208 1308
Fragments arranged by size:
3524 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 3100 3878 4068 4860 3100
Size: 778 190 792 4326
Fragments arranged by size:
4326 792 778 190
BsmI GAATG_Cn'
Cuts at: 2906 2979 2906
Size: 73 6013
Bsp1286I G_dGCh'C
Cuts at: 1373 3100 3711 3804 3878 4068 4130 4860 1373
Size: 1727 611 93 74 190 62 730 2599
Fragments arranged by size:
2599 1727 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 5306 5306
Size: 6086
BspHI T'CATG_A
Cuts at: 2718 2769 5262 2718
Size: 51 2493 3542
Fragments arranged by size:
3542 2493 51
BspMI ACCTGCnnnn'nnnn_
Cuts at: 309 3136 3350 3652 4033 309
Size: 2827 214 302 381 2362
Fragments arranged by size:
2827 2362 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4378 4432 5916 6081 50
Size: 4328 54 1484 165 55
Fragments arranged by size:
4328 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 631 1334 2204 3998 631
Size: 703 870 1794 2719
Fragments arranged by size:
2719 1794 870 703
BsrFI r'CCGG_y
Cuts at: 4084 4265 4474 4084
Size: 181 209 5696
Fragments arranged by size:
5696 209 181
BssSI C'ACGA_G
Cuts at: 347 2828 4357 4715 347
Size: 2481 1529 358 1718
Fragments arranged by size:
2481 1718 1529 358
BstAPI GCAn_nnn'nTGC
Cuts at: 394 1953 3463 394
Size: 1559 1510 3017
Fragments arranged by size:
3017 1559 1510
BstBI TT'CG_AA
Cuts at: 1069 4447 1069
Size: 3378 2708
BstYI r'GATC_y
Cuts at: 1 741 771 1978 3111 3578 3601 3936 4182
Size: 740 30 1207 1133 467 23 335 246
Cuts at: 4182 5194 5280 5292 5638 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1207 1133 1012 740 467 449 346 335
246 86 30 23 12
Bsu36I CC'TnA_GG
Cuts at: 1973 1973
Size: 6086
BtgI C'CryG_G
Cuts at: 107 107
Size: 6086
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1621 4036 4217 4380 1621
Size: 2415 181 163 3327
Fragments arranged by size:
3327 2415 181 163
BtsI GCAGTG_nn'
Cuts at: 244 2243 2525 3329 244
Size: 1999 282 804 3001
Fragments arranged by size:
3001 1999 804 282
DraI TTT'AAA
Cuts at: 1131 3085 1131
Size: 1954 4132
DrdI GACnn_nn'nnGTC
Cuts at: 3792 4650 3792
Size: 858 5228
EaeI y'GGCC_r
Cuts at: 1095 3038 3671 3845 4236 4263 5608 1095
Size: 1943 633 174 391 27 1345 1573
Fragments arranged by size:
1943 1573 1345 633 391 174 27
EagI C'GGCC_G
Cuts at: 3671 3671
Size: 6086
EarI CTCTTCn'nnn_
Cuts at: 684 2071 2159 2445 4109 4319 684
Size: 1387 88 286 1664 210 2451
Fragments arranged by size:
2451 1664 1387 286 210 88
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4294 4604 4750 5896 4294
Size: 310 146 1146 4484
Fragments arranged by size:
4484 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3098 3098
Size: 6086
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 665 775 1469 1484 2587 2656 2938 3911 4343
Size: 110 694 15 1103 69 282 973 432
Cuts at: 4343 5090 5358 665
Size: 747 268 1393
Fragments arranged by size:
1393 1103 973 747 694 432 282 268
110 69 15
EcoO109I rG'GnC_Cy
Cuts at: 3225 3225
Size: 6086
EcoRI G'AATT_C
Cuts at: 2602 3090 2602
Size: 488 5598
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 718 2551 4105 718
Size: 1833 1554 2699
Fragments arranged by size:
2699 1833 1554
FspI TGC'GCA
Cuts at: 3867 3867
Size: 6086
HaeII r_GCGC'y
Cuts at: 3520 3768 4790 5769 3520
Size: 248 1022 979 3837
Fragments arranged by size:
3837 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 218 250 3928 3958 3960 3990 218
Size: 32 3678 30 2 30 2314
Fragments arranged by size:
3678 2314 32 30 30 2
HincII GTy'rAC
Cuts at: 324 947 1559 1992 3125 324
Size: 623 612 433 1133 3285
Fragments arranged by size:
3285 1133 623 612 433
HindIII A'AGCT_T
Cuts at: 335 520 662 335
Size: 185 142 5759
Fragments arranged by size:
5759 185 142
HpaI GTT'AAC
Cuts at: 324 1559 1992 324
Size: 1235 433 4418
Fragments arranged by size:
4418 1235 433
KasI G'GCGC_C
Cuts at: 3516 3764 3516
Size: 248 5838
KpnI G_GTAC'C
Cuts at: 3106 3106
Size: 6086
MluI A'CGCG_T
Cuts at: 5519 5519
Size: 6086
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2201 4757 4941 2201
Size: 2556 184 3346
Fragments arranged by size:
3346 2556 184
MscI TGG'CCA
Cuts at: 1097 3847 1097
Size: 2750 3336
MslI CAynn'nnrTG
Cuts at: 1045 1260 1366 1807 4075 4202 1045
Size: 215 106 441 2268 127 2929
Fragments arranged by size:
2929 2268 441 215 127 106
MspA1I CmG'CkG
Cuts at: 2206 2746 2845 3197 3511 3871 4884 5129 2206
Size: 540 99 352 314 360 1013 245 3163
Fragments arranged by size:
3163 1013 540 360 352 314 245 99
NaeI GCC'GGC
Cuts at: 4267 4267
Size: 6086
NarI GG'CG_CC
Cuts at: 3517 3765 3517
Size: 248 5838
NcoI C'CATG_G
Cuts at: 107 107
Size: 6086
NdeI CA'TA_TG
Cuts at: 1188 1629 1188
Size: 441 5645
NgoMIV G'CCGG_C
Cuts at: 4265 4265
Size: 6086
NheI G'CTAG_C
Cuts at: 1060 1060
Size: 6086
NspI r_CATG'y
Cuts at: 3139 3639 4546 3139
Size: 500 907 4679
Fragments arranged by size:
4679 907 500
PciI A'CATG_T
Cuts at: 4542 4542
Size: 6086
PflMI CCAn_nnn'nTGG
Cuts at: 1663 1663
Size: 6086
PfoI T'CCnGG_A
Cuts at: 353 1246 353
Size: 893 5193
PmeI GTTT'AAAC
Cuts at: 3085 3085
Size: 6086
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5225 5257 5225
Size: 32 6054
PshAI GACnn'nnGTC
Cuts at: 349 349
Size: 6086
PsiI TTA'TAA
Cuts at: 1276 1276
Size: 6086
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 109 141 109
Size: 32 6054
PstI C_TGCA'G
Cuts at: 516 1714 2520 3133 3818 516
Size: 1198 806 613 685 2784
Fragments arranged by size:
2784 1198 806 685 613
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6086
PvuII CAG'CTG
Cuts at: 2746 2845 3511 3871 2746
Size: 99 666 360 4961
Fragments arranged by size:
4961 666 360 99
RsrII CG'GwC_CG
Cuts at: 4281 4281
Size: 6086
SacI G_AGCT'C
Cuts at: 3100 3100
Size: 6086
SalI G'TCGA_C
Cuts at: 3123 3123
Size: 6086
SapI GCTCTTCn'nnn_
Cuts at: 2159 2445 4109 4319 2159
Size: 286 1664 210 3926
Fragments arranged by size:
3926 1664 286 210
SbfI CC_TGCA'GG
Cuts at: 3133 3133
Size: 6086
ScaI AGT'ACT
Cuts at: 5603 5603
Size: 6086
SfcI C'TryA_G
Cuts at: 33 512 1710 2516 3129 3814 4807 4998 33
Size: 479 1198 806 613 685 993 191 1121
Fragments arranged by size:
1198 1121 993 806 685 613 479 191
SfoI GGC'GCC
Cuts at: 3518 3766 3518
Size: 248 5838
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6086
SmaI CCC'GGG
Cuts at: 3108 3108
Size: 6086
SmlI C'TyrA_G
Cuts at: 1028 1681 2294 2471 2657 3241 4156 4648 4910
Size: 653 613 177 186 584 915 492 262
Cuts at: 4910 1028
Size: 2204
Fragments arranged by size:
2204 915 653 613 584 492 262 186
177
SpeI A'CTAG_T
Cuts at: 67 2014 2410 67
Size: 1947 396 3743
Fragments arranged by size:
3743 1947 396
SphI G_CATG'C
Cuts at: 3139 3639 3139
Size: 500 5586
SspI AAT'ATT
Cuts at: 642 642
Size: 6086
StuI AGG'CCT
Cuts at: 2442 2442
Size: 6086
StyI C'CwwG_G
Cuts at: 107 1014 2964 3220 3574 107
Size: 907 1950 256 354 2619
Fragments arranged by size:
2619 1950 907 354 256
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3669 3802 4261 4470 3669
Size: 133 459 209 5285
Fragments arranged by size:
5285 459 209 133
TatI w'GTAC_w
Cuts at: 5601 5601
Size: 6086
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5603 5635 5603
Size: 32 6054
Tth111I GACn'n_nGTC
Cuts at: 845 3883 845
Size: 3038 3048
XbaI T'CTAG_A
Cuts at: 838 1110 3117 838
Size: 272 2007 3807
Fragments arranged by size:
3807 2007 272
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2393 3581 2393
Size: 1188 4898
XmaI C'CCGG_G
Cuts at: 3106 3106
Size: 6086
XmnI GAAnn'nnTTC
Cuts at: 665 1242 2016 665
Size: 577 774 4735
Fragments arranged by size:
4735 774 577
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NspI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BpmI BsgI
BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI MfeI NotI
NruI NsiI PacI PasI PmlI PpuMI PspOMI PspXI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI
XhoI ZraI