Restriction map for
pF1KA0424
(Circular) (Six-base) MAPSORT of: pF1KA0424.seq Check: 6925 from: 1 to: 4661
pF1KA0424 4661 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AatII G_ACGT'C
Cuts at: 209 209
Size: 4661
AccI GT'mk_AC
Cuts at: 883 1699 883
Size: 816 3845
Acc65I G'GTAC_C
Cuts at: 1677 1677
Size: 4661
AclI AA'CG_TT
Cuts at: 4501 4501
Size: 4661
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 517 562 1614 2486 2918 3665 3933 517
Size: 45 1052 872 432 747 268 1245
Fragments arranged by size:
1245 1052 872 747 432 268 45
AflIII A'CryG_T
Cuts at: 521 2013 3117 4094 521
Size: 1492 1104 977 1088
Fragments arranged by size:
1492 1104 1088 977
AgeI A'CCGG_T
Cuts at: 3049 3049
Size: 4661
AlwNI CAG_nnn'CTG
Cuts at: 1548 1966 3533 1548
Size: 418 1567 2676
Fragments arranged by size:
2676 1567 418
ApaI G_GGCC'C
Cuts at: 335 335
Size: 4661
ApaLI G'TGCA_C
Cuts at: 3431 3431
Size: 4661
ApoI r'AATT_y
Cuts at: 1415 1665 4014 4270 1415
Size: 250 2349 256 1806
Fragments arranged by size:
2349 1806 256 250
AseI AT'TA_AT
Cuts at: 20 20
Size: 4661
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4661
AvaI C'yCGr_G
Cuts at: 1681 1681
Size: 4661
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1303 1336 1303
Size: 33 4628
BamHI G'GATC_C
Cuts at: 1686 1686
Size: 4661
BanI G'GyrC_C
Cuts at: 1677 2091 2339 2374 1677
Size: 414 248 35 3964
Fragments arranged by size:
3964 414 248 35
BanII G_rGCy'C
Cuts at: 335 731 875 1057 1675 2705 335
Size: 396 144 182 618 1030 2291
Fragments arranged by size:
2291 1030 618 396 182 144
BbeI G_GCGC'C
Cuts at: 2095 2343 2095
Size: 248 4413
BbsI GAAGACnn'nnnn_
Cuts at: 1116 1116
Size: 4661
BbvCI CC'TCA_GC
Cuts at: 146 637 146
Size: 491 4170
BciVI GTATCCnnnnn_n'
Cuts at: 863 2559 3320 4661 863
Size: 1696 761 1341 863
Fragments arranged by size:
1696 1341 863 761
BclI T'GATC_A
Cuts at: 119 2181 119
Size: 2062 2599
BglII A'GATC_T
Cuts at: 842 2176 4213 842
Size: 1334 2037 1290
Fragments arranged by size:
2037 1334 1290
BlpI GC'TnA_GC
Cuts at: 869 1773 869
Size: 904 3757
Bme1580I G_kGCm'C
Cuts at: 335 2286 2379 3435 335
Size: 1951 93 1056 1561
Fragments arranged by size:
1951 1561 1056 93
BmgBI CAC'GTC
Cuts at: 165 165
Size: 4661
BmrI ACTGGGnnnn_n'
Cuts at: 1035 1082 1873 1954 2289 4433 1035
Size: 47 791 81 335 2144 1263
Fragments arranged by size:
2144 1263 791 335 81 47
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3028 3028
Size: 4661
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 146 625 815 1357 146
Size: 479 190 542 3450
Fragments arranged by size:
3450 542 479 190
Bpu10I CC'TnA_GC
Cuts at: 146 637 1357 1466 146
Size: 491 720 109 3341
Fragments arranged by size:
3341 720 491 109
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 1837 2716 3208 3506 457
Size: 1380 879 492 298 1612
Fragments arranged by size:
1612 1380 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 593 4365 593
Size: 3772 889
BsaAI yAC'GTr
Cuts at: 2014 2014
Size: 4661
BsaBI GATnn'nnATC
Cuts at: 6 2175 4212 6
Size: 2169 2037 455
Fragments arranged by size:
2169 2037 455
BsaHI Gr'CG_yC
Cuts at: 206 2092 2340 3042 4538 206
Size: 1886 248 702 1496 329
Fragments arranged by size:
1886 1496 702 329 248
BsaWI w'CCGG_w
Cuts at: 1172 1850 2371 3049 3323 3470 3881 1172
Size: 678 521 678 274 147 411 1952
Fragments arranged by size:
1952 678 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 259 289 598 628 259
Size: 30 309 30 4292
Fragments arranged by size:
4292 309 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 280 340 1321 280
Size: 60 981 3620
Fragments arranged by size:
3620 981 60
BseYI C'CCAG_C
Cuts at: 334 2086 2844 3421 3917 334
Size: 1752 758 577 496 1078
Fragments arranged by size:
1752 1078 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 856 364
Size: 492 4169
BsiEI CG_ry'CG
Cuts at: 104 1107 2203 2249 3457 104
Size: 1003 1096 46 1208 1308
Fragments arranged by size:
1308 1208 1096 1003 46
BsiHKAI G_wGCw'C
Cuts at: 448 668 731 875 1057 1675 2453 2643 3435
Size: 220 63 144 182 618 778 190 792
Cuts at: 3435 448
Size: 1674
Fragments arranged by size:
1674 792 778 618 220 190 182 144
63
BsmI GAATG_Cn'
Cuts at: 1281 1281
Size: 4661
Bsp1286I G_dGCh'C
Cuts at: 335 448 668 731 875 1057 1675 2286 2379
Size: 113 220 63 144 182 618 611 93
Cuts at: 2379 2453 2643 2705 3435 335
Size: 74 190 62 730 1561
Fragments arranged by size:
1561 730 618 611 220 190 182 144
113 93 74 63 62
BspEI T'CCGG_A
Cuts at: 1172 3881 1172
Size: 2709 1952
BspHI T'CATG_A
Cuts at: 935 3837 935
Size: 2902 1759
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1566 1625 1711 1925 2227 2608 1566
Size: 59 86 214 302 381 3619
Fragments arranged by size:
3619 381 302 214 86 59
BsrBI CCG'CTC
Cuts at: 50 1493 2953 3007 4491 4656 50
Size: 1443 1460 54 1484 165 55
Fragments arranged by size:
1484 1460 1443 165 55 54
BsrDI GCAATG_nn'
Cuts at: 803 1451 2573 803
Size: 648 1122 2891
Fragments arranged by size:
2891 1122 648
BsrFI r'CCGG_y
Cuts at: 2659 2840 3049 2659
Size: 181 209 4271
Fragments arranged by size:
4271 209 181
BssSI C'ACGA_G
Cuts at: 2932 3290 2932
Size: 358 4303
BstAPI GCAn_nnn'nTGC
Cuts at: 2038 2038
Size: 4661
BstBI TT'CG_AA
Cuts at: 3022 3022
Size: 4661
BstXI CCAn_nnnn'nTGG
Cuts at: 1335 1335
Size: 4661
BstYI r'GATC_y
Cuts at: 1 842 1088 1686 2153 2176 2511 2757 3769
Size: 841 246 598 467 23 335 246 1012
Cuts at: 3769 3855 3867 4213 1
Size: 86 12 346 449
Fragments arranged by size:
1012 841 598 467 449 346 335 246
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 884 884
Size: 4661
BtgI C'CryG_G
Cuts at: 170 1536 170
Size: 1366 3295
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 353 1548 2611 2792 2955 353
Size: 1195 1063 181 163 2059
Fragments arranged by size:
2059 1195 1063 181 163
BtsI GCAGTG_nn'
Cuts at: 508 1904 508
Size: 1396 3265
ClaI AT'CG_AT
Cuts at: 812 812
Size: 4661
DraI TTT'AAA
Cuts at: 195 1660 195
Size: 1465 3196
DrdI GACnn_nn'nnGTC
Cuts at: 1261 2367 3225 1261
Size: 1106 858 2697
Fragments arranged by size:
2697 1106 858
EaeI y'GGCC_r
Cuts at: 173 391 1104 1539 2246 2420 2811 2838 4183
Size: 218 713 435 707 174 391 27 1345
Cuts at: 4183 173
Size: 651
Fragments arranged by size:
1345 713 707 651 435 391 218 174
27
EagI C'GGCC_G
Cuts at: 1104 2246 1104
Size: 1142 3519
EarI CTCTTCn'nnn_
Cuts at: 505 1367 1426 2684 2894 505
Size: 862 59 1258 210 2272
Fragments arranged by size:
2272 1258 862 210 59
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2869 3179 3325 4471 2869
Size: 310 146 1146 3059
Fragments arranged by size:
3059 1146 310 146
EcoICRI GAG'CTC
Cuts at: 729 873 1055 1673 729
Size: 144 182 618 3717
Fragments arranged by size:
3717 618 182 144
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 146 517 562 625 815 1357 1614 2486 2918
Size: 371 45 63 190 542 257 872 432
Cuts at: 2918 3665 3933 146
Size: 747 268 874
Fragments arranged by size:
874 872 747 542 432 371 268 257
190 63 45
EcoO109I rG'GnC_Cy
Cuts at: 331 649 1164 1524 1800 331
Size: 318 515 360 276 3192
Fragments arranged by size:
3192 515 360 318 276
EcoRI G'AATT_C
Cuts at: 1665 1665
Size: 4661
EcoRV GAT'ATC
Cuts at: 570 570
Size: 4661
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 567 873 1296 2680 567
Size: 306 423 1384 2548
Fragments arranged by size:
2548 1384 423 306
FspI TGC'GCA
Cuts at: 2442 2442
Size: 4661
HaeII r_GCGC'y
Cuts at: 2095 2343 3365 4344 2095
Size: 248 1022 979 2412
Fragments arranged by size:
2412 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 119 151 1039 1071 2503 2533 2535 2565 119
Size: 32 888 32 1432 30 2 30 2215
Fragments arranged by size:
2215 1432 888 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1230 1482 1640 1700 1230
Size: 252 158 60 4191
Fragments arranged by size:
4191 252 158 60
HindIII A'AGCT_T
Cuts at: 1285 1285
Size: 4661
HpaI GTT'AAC
Cuts at: 1640 1640
Size: 4661
KasI G'GCGC_C
Cuts at: 2091 2339 2091
Size: 248 4413
KpnI G_GTAC'C
Cuts at: 1681 1681
Size: 4661
MluI A'CGCG_T
Cuts at: 4094 4094
Size: 4661
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 254 3332 3516 254
Size: 3078 184 1399
Fragments arranged by size:
3078 1399 184
MscI TGG'CCA
Cuts at: 175 2422 175
Size: 2247 2414
MslI CAynn'nnrTG
Cuts at: 671 713 1207 2650 2777 671
Size: 42 494 1443 127 2555
Fragments arranged by size:
2555 1443 494 127 42
MspA1I CmG'CkG
Cuts at: 922 1119 1772 2086 2446 3459 3704 922
Size: 197 653 314 360 1013 245 1879
Fragments arranged by size:
1879 1013 653 360 314 245 197
NaeI GCC'GGC
Cuts at: 2842 2842
Size: 4661
NarI GG'CG_CC
Cuts at: 2092 2340 2092
Size: 248 4413
NcoI C'CATG_G
Cuts at: 170 170
Size: 4661
NgoMIV G'CCGG_C
Cuts at: 2840 2840
Size: 4661
NspI r_CATG'y
Cuts at: 525 1714 2214 3121 525
Size: 1189 500 907 2065
Fragments arranged by size:
2065 1189 907 500
PciI A'CATG_T
Cuts at: 521 3117 521
Size: 2596 2065
PflMI CCAn_nnn'nTGG
Cuts at: 1146 1548 1146
Size: 402 4259
PmeI GTTT'AAAC
Cuts at: 1660 1660
Size: 4661
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3800 3832 3800
Size: 32 4629
PpuMI rG'GwC_Cy
Cuts at: 649 1164 1524 649
Size: 515 360 3786
Fragments arranged by size:
3786 515 360
PspOMI G'GGCC_C
Cuts at: 331 331
Size: 4661
PstI C_TGCA'G
Cuts at: 1708 2393 1708
Size: 685 3976
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4661
PvuII CAG'CTG
Cuts at: 922 2086 2446 922
Size: 1164 360 3137
Fragments arranged by size:
3137 1164 360
RsrII CG'GwC_CG
Cuts at: 2856 2856
Size: 4661
SacI G_AGCT'C
Cuts at: 731 875 1057 1675 731
Size: 144 182 618 3717
Fragments arranged by size:
3717 618 182 144
SalI G'TCGA_C
Cuts at: 1698 1698
Size: 4661
SapI GCTCTTCn'nnn_
Cuts at: 2684 2894 2684
Size: 210 4451
SbfI CC_TGCA'GG
Cuts at: 1708 1708
Size: 4661
ScaI AGT'ACT
Cuts at: 4178 4178
Size: 4661
SexAI A'CCwGG_T
Cuts at: 1547 1547
Size: 4661
SfcI C'TryA_G
Cuts at: 33 397 573 906 1704 2389 3382 3573 33
Size: 364 176 333 798 685 993 191 1121
Fragments arranged by size:
1121 993 798 685 364 333 191 176
SfoI GGC'GCC
Cuts at: 2093 2341 2093
Size: 248 4413
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4661
SmaI CCC'GGG
Cuts at: 1683 1683
Size: 4661
SmlI C'TyrA_G
Cuts at: 472 1816 2731 3223 3485 472
Size: 1344 915 492 262 1648
Fragments arranged by size:
1648 1344 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4661
SphI G_CATG'C
Cuts at: 1714 2214 1714
Size: 500 4161
SspI AAT'ATT
Cuts at: 987 987
Size: 4661
StuI AGG'CCT
Cuts at: 775 775
Size: 4661
StyI C'CwwG_G
Cuts at: 170 1795 2149 170
Size: 1625 354 2682
Fragments arranged by size:
2682 1625 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 291 2244 2377 2836 3045 291
Size: 1953 133 459 209 1907
Fragments arranged by size:
1953 1907 459 209 133
TatI w'GTAC_w
Cuts at: 1187 4176 1187
Size: 2989 1672
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 229 261 4178 4210 229
Size: 32 3917 32 680
Fragments arranged by size:
3917 680 32 32
Tth111I GACn'n_nGTC
Cuts at: 2458 2458
Size: 4661
XbaI T'CTAG_A
Cuts at: 1692 1692
Size: 4661
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 183 2156 183
Size: 1973 2688
XmaI C'CCGG_G
Cuts at: 1681 1681
Size: 4661
ZraI GAC'GTC
Cuts at: 207 207
Size: 4661
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BcgI BfrBI BglI BmtI BsiWI BsmBI BsrGI
BssHII BstEII Bsu36I CspCI DraIII EcoNI FseI FspAI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PshAI PsiI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SwaI XhoI XmnI