Restriction map for
pF1KA0425
(Circular) (Six-base) MAPSORT of: pF1KA0425.seq Check: 7010 from: 1 to: 6785
pF1KA0425 6785 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 273 844 273
Size: 571 6214
AccI GT'mk_AC
Cuts at: 214 930 3823 214
Size: 716 2893 3176
Fragments arranged by size:
3176 2893 716
Acc65I G'GTAC_C
Cuts at: 3801 3801
Size: 6785
AclI AA'CG_TT
Cuts at: 6625 6625
Size: 6785
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 110 628 989 1733 2968 3397 3773 4610 5042
Size: 518 361 744 1235 429 376 837 432
Cuts at: 5042 5789 6057 110
Size: 747 268 838
Fragments arranged by size:
1235 838 837 747 744 518 432 429
376 361 268
AfeI AGC'GCT
Cuts at: 3625 3625
Size: 6785
AflIII A'CryG_T
Cuts at: 2756 3200 3700 4137 5241 6218 2756
Size: 444 500 437 1104 977 3323
Fragments arranged by size:
3323 1104 977 500 444 437
AgeI A'CCGG_T
Cuts at: 5173 5173
Size: 6785
AhdI GACnn_n'nnGTC
Cuts at: 2961 2961
Size: 6785
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 242 274 2144 2176 242
Size: 32 1870 32 4851
Fragments arranged by size:
4851 1870 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1375 2547 3087 4090 5657 1375
Size: 1172 540 1003 1567 2503
Fragments arranged by size:
2503 1567 1172 1003 540
ApaLI G'TGCA_C
Cuts at: 5555 5555
Size: 6785
ApoI r'AATT_y
Cuts at: 972 999 1842 2153 3074 3789 6138 6394 972
Size: 27 843 311 921 715 2349 256 1363
Fragments arranged by size:
2349 1363 921 843 715 311 256 27
AseI AT'TA_AT
Cuts at: 20 20
Size: 6785
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6785
AvaI C'yCGr_G
Cuts at: 3805 3805
Size: 6785
BamHI G'GATC_C
Cuts at: 3810 3810
Size: 6785
BanI G'GyrC_C
Cuts at: 2079 3801 4215 4463 4498 2079
Size: 1722 414 248 35 4366
Fragments arranged by size:
4366 1722 414 248 35
BanII G_rGCy'C
Cuts at: 2696 3128 3799 4829 2696
Size: 432 671 1030 4652
Fragments arranged by size:
4652 1030 671 432
BbeI G_GCGC'C
Cuts at: 4219 4467 4219
Size: 248 6537
BbsI GAAGACnn'nnnn_
Cuts at: 796 2054 2237 2323 2960 3761 796
Size: 1258 183 86 637 801 3820
Fragments arranged by size:
3820 1258 801 637 183 86
BbvCI CC'TCA_GC
Cuts at: 753 888 753
Size: 135 6650
BciVI GTATCCnnnnn_n'
Cuts at: 4683 5444 6785 4683
Size: 761 1341 4683
Fragments arranged by size:
4683 1341 761
BclI T'GATC_A
Cuts at: 341 4305 341
Size: 3964 2821
BfrBI ATG'CAT
Cuts at: 1541 1541
Size: 6785
BglII A'GATC_T
Cuts at: 4300 6337 4300
Size: 2037 4748
BlpI GC'TnA_GC
Cuts at: 1829 3897 1829
Size: 2068 4717
Bme1580I G_kGCm'C
Cuts at: 351 1016 1821 4410 4503 5559 351
Size: 665 805 2589 93 1056 1577
Fragments arranged by size:
2589 1577 1056 805 665 93
BmrI ACTGGGnnnn_n'
Cuts at: 345 1143 2793 3535 3997 4078 4413 6557 345
Size: 798 1650 742 462 81 335 2144 573
Fragments arranged by size:
2144 1650 798 742 573 462 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5152 5152
Size: 6785
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 1117 1769 2123 163
Size: 954 652 354 4825
Fragments arranged by size:
4825 954 652 354
Bpu10I CC'TnA_GC
Cuts at: 753 888 3620 753
Size: 135 2732 3918
Fragments arranged by size:
3918 2732 135
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2299 2870 2888 3961 4840 5332 5630 2299
Size: 571 18 1073 879 492 298 3454
Fragments arranged by size:
3454 1073 879 571 492 298 18
BsaI GGTCTCn'nnnn_
Cuts at: 1135 3015 6489 1135
Size: 1880 3474 1431
Fragments arranged by size:
3474 1880 1431
BsaAI yAC'GTr
Cuts at: 3703 4138 3703
Size: 435 6350
BsaBI GATnn'nnATC
Cuts at: 6 4299 6336 6
Size: 4293 2037 455
Fragments arranged by size:
4293 2037 455
BsaHI Gr'CG_yC
Cuts at: 4216 4464 5166 6662 4216
Size: 248 702 1496 4339
Fragments arranged by size:
4339 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3974 4495 5173 5447 5594 6005 3974
Size: 521 678 274 147 411 4754
Fragments arranged by size:
4754 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1499 1529 1499
Size: 30 6755
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 272 1088 272
Size: 816 5969
BseYI C'CCAG_C
Cuts at: 1154 1436 2447 3246 4210 4968 5545 6041 1154
Size: 282 1011 799 964 758 577 496 1898
Fragments arranged by size:
1898 1011 964 799 758 577 496 282
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1479 1886 2952 1479
Size: 407 1066 5312
Fragments arranged by size:
5312 1066 407
BsiEI CG_ry'CG
Cuts at: 104 4327 4373 5581 104
Size: 4223 46 1208 1308
Fragments arranged by size:
4223 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 3332 3799 4577 4767 5559 3332
Size: 467 778 190 792 4558
Fragments arranged by size:
4558 792 778 467 190
BsmI GAATG_Cn'
Cuts at: 489 1543 2041 489
Size: 1054 498 5233
Fragments arranged by size:
5233 1054 498
BsmBI CGTCTCn'nnnn_
Cuts at: 1144 3354 1144
Size: 2210 4575
Bsp1286I G_dGCh'C
Cuts at: 351 1016 1821 2696 3128 3332 3799 4410 4503
Size: 665 805 875 432 204 467 611 93
Cuts at: 4503 4577 4767 4829 5559 351
Size: 74 190 62 730 1577
Fragments arranged by size:
1577 875 805 730 665 611 467 432
204 190 93 74 62
BspEI T'CCGG_A
Cuts at: 6005 6005
Size: 6785
BspHI T'CATG_A
Cuts at: 5961 5961
Size: 6785
BspMI ACCTGCnnnn'nnnn_
Cuts at: 273 844 1664 3835 4049 4351 4732 273
Size: 571 820 2171 214 302 381 2326
Fragments arranged by size:
2326 2171 820 571 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2936 5077 5131 6615 6780 50
Size: 2886 2141 54 1484 165 55
Fragments arranged by size:
2886 2141 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1322 4697 1322
Size: 3375 3410
BsrFI r'CCGG_y
Cuts at: 4783 4964 5173 4783
Size: 181 209 6395
Fragments arranged by size:
6395 209 181
BsrGI T'GTAC_A
Cuts at: 858 858
Size: 6785
BssSI C'ACGA_G
Cuts at: 5056 5414 5056
Size: 358 6427
BstAPI GCAn_nnn'nTGC
Cuts at: 941 4162 941
Size: 3221 3564
BstBI TT'CG_AA
Cuts at: 5146 5146
Size: 6785
BstXI CCAn_nnnn'nTGG
Cuts at: 783 1162 2447 783
Size: 379 1285 5121
Fragments arranged by size:
5121 1285 379
BstYI r'GATC_y
Cuts at: 1 748 2820 3810 4277 4300 4635 4881 5893
Size: 747 2072 990 467 23 335 246 1012
Cuts at: 5893 5979 5991 6337 1
Size: 86 12 346 449
Fragments arranged by size:
2072 1012 990 747 467 449 346 335
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 931 931
Size: 6785
Bsu36I CC'TnA_GG
Cuts at: 2874 2874
Size: 6785
BtgI C'CryG_G
Cuts at: 1733 1733
Size: 6785
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4735 4916 5079 4735
Size: 181 163 6441
Fragments arranged by size:
6441 181 163
BtsI GCAGTG_nn'
Cuts at: 543 4028 543
Size: 3485 3300
DraI TTT'AAA
Cuts at: 326 3275 3784 326
Size: 2949 509 3327
Fragments arranged by size:
3327 2949 509
DraIII CAC_nnn'GTG
Cuts at: 239 727 1177 239
Size: 488 450 5847
Fragments arranged by size:
5847 488 450
DrdI GACnn_nn'nnGTC
Cuts at: 4491 5349 4491
Size: 858 5927
EaeI y'GGCC_r
Cuts at: 1574 4370 4544 4935 4962 6307 1574
Size: 2796 174 391 27 1345 2052
Fragments arranged by size:
2796 2052 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4370 4370
Size: 6785
EarI CTCTTCn'nnn_
Cuts at: 1514 2120 2303 2450 2655 2804 3215 4808 5018
Size: 606 183 147 205 149 411 1593 210
Cuts at: 5018 1514
Size: 3281
Fragments arranged by size:
3281 1593 606 411 210 205 183 149
147
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4993 5303 5449 6595 4993
Size: 310 146 1146 5183
Fragments arranged by size:
5183 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3797 3797
Size: 6785
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 110 163 628 989 1117 1733 1769 2123 2968
Size: 53 465 361 128 616 36 354 845
Cuts at: 2968 3397 3773 4610 5042 5789 6057 110
Size: 429 376 837 432 747 268 838
Fragments arranged by size:
845 838 837 747 616 465 432 429
376 361 354 268 128 53 36
EcoNI CCTnn'n_nnAGG
Cuts at: 181 181
Size: 6785
EcoO109I rG'GnC_Cy
Cuts at: 1756 2544 3924 1756
Size: 788 1380 4617
Fragments arranged by size:
4617 1380 788
EcoRI G'AATT_C
Cuts at: 999 1842 2153 3074 3789 999
Size: 843 311 921 715 3995
Fragments arranged by size:
3995 921 843 715 311
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2263 3360 4804 2263
Size: 1097 1444 4244
Fragments arranged by size:
4244 1444 1097
FspI TGC'GCA
Cuts at: 4566 4566
Size: 6785
HaeII r_GCGC'y
Cuts at: 3254 3627 4219 4467 5489 6468 3254
Size: 373 592 248 1022 979 3571
Fragments arranged by size:
3571 1022 979 592 373 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 643 675 2310 2342 2404 2416 2436 2448 2548
Size: 32 1635 32 62 12 20 12 100
Cuts at: 2548 2580 2585 2617 2947 2979 3357 3384 3389
Size: 32 5 32 330 32 378 27 5
Cuts at: 3389 3416 3527 3559 4627 4657 4659 4689 643
Size: 27 111 32 1068 30 2 30 2739
Fragments arranged by size:
2739 1635 1068 378 330 111 100 62
32 32 32 32 32 32 30 30
27 27 20 12 12 5 5 2
HincII GTy'rAC
Cuts at: 404 1198 3698 3824 404
Size: 794 2500 126 3365
Fragments arranged by size:
3365 2500 794 126
HindIII A'AGCT_T
Cuts at: 1967 2245 3337 1967
Size: 278 1092 5415
Fragments arranged by size:
5415 1092 278
HpaI GTT'AAC
Cuts at: 3698 3698
Size: 6785
KasI G'GCGC_C
Cuts at: 4215 4463 4215
Size: 248 6537
KpnI G_GTAC'C
Cuts at: 3805 3805
Size: 6785
MluI A'CGCG_T
Cuts at: 6218 6218
Size: 6785
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1897 3333 3570 5456 5640 1897
Size: 1436 237 1886 184 3042
Fragments arranged by size:
3042 1886 1436 237 184
MscI TGG'CCA
Cuts at: 1576 4546 1576
Size: 2970 3815
MslI CAynn'nnrTG
Cuts at: 2636 3534 4774 4901 2636
Size: 898 1240 127 4520
Fragments arranged by size:
4520 1240 898 127
MspA1I CmG'CkG
Cuts at: 142 953 1126 3246 3896 4210 4570 5583 5828
Size: 811 173 2120 650 314 360 1013 245
Cuts at: 5828 142
Size: 1099
Fragments arranged by size:
2120 1099 1013 811 650 360 314 245
173
NaeI GCC'GGC
Cuts at: 4966 4966
Size: 6785
NarI GG'CG_CC
Cuts at: 4216 4464 4216
Size: 248 6537
NcoI C'CATG_G
Cuts at: 1733 1733
Size: 6785
NdeI CA'TA_TG
Cuts at: 408 408
Size: 6785
NgoMIV G'CCGG_C
Cuts at: 4964 4964
Size: 6785
NsiI A_TGCA'T
Cuts at: 1543 1543
Size: 6785
NspI r_CATG'y
Cuts at: 474 642 1162 1545 2760 3204 3838 4338 5245
Size: 168 520 383 1215 444 634 500 907
Cuts at: 5245 474
Size: 2014
Fragments arranged by size:
2014 1215 907 634 520 500 444 383
168
PciI A'CATG_T
Cuts at: 2756 3200 5241 2756
Size: 444 2041 4300
Fragments arranged by size:
4300 2041 444
PflMI CCAn_nnn'nTGG
Cuts at: 519 519
Size: 6785
PfoI T'CCnGG_A
Cuts at: 2737 2737
Size: 6785
PmeI GTTT'AAAC
Cuts at: 3784 3784
Size: 6785
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2342 2374 5924 5956 2342
Size: 32 3550 32 3171
Fragments arranged by size:
3550 3171 32 32
PpuMI rG'GwC_Cy
Cuts at: 1756 2544 1756
Size: 788 5997
PsiI TTA'TAA
Cuts at: 1415 1415
Size: 6785
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3167 3199 3167
Size: 32 6753
PstI C_TGCA'G
Cuts at: 855 946 1125 3832 4517 855
Size: 91 179 2707 685 3123
Fragments arranged by size:
3123 2707 685 179 91
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6785
PvuII CAG'CTG
Cuts at: 142 953 1126 3246 4210 4570 142
Size: 811 173 2120 964 360 2357
Fragments arranged by size:
2357 2120 964 811 360 173
RsrII CG'GwC_CG
Cuts at: 4980 4980
Size: 6785
SacI G_AGCT'C
Cuts at: 3799 3799
Size: 6785
SalI G'TCGA_C
Cuts at: 3822 3822
Size: 6785
SapI GCTCTTCn'nnn_
Cuts at: 2450 2804 3215 4808 5018 2450
Size: 354 411 1593 210 4217
Fragments arranged by size:
4217 1593 411 354 210
SbfI CC_TGCA'GG
Cuts at: 3832 3832
Size: 6785
ScaI AGT'ACT
Cuts at: 1908 2883 6302 1908
Size: 975 3419 2391
Fragments arranged by size:
3419 2391 975
SfcI C'TryA_G
Cuts at: 33 851 942 948 1121 1389 1835 2678 2925
Size: 818 91 6 173 268 446 843 247
Cuts at: 2925 3828 4513 5506 5697 33
Size: 903 685 993 191 1121
Fragments arranged by size:
1121 993 903 843 818 685 446 268
247 191 173 91 6
SfoI GGC'GCC
Cuts at: 4217 4465 4217
Size: 248 6537
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6785
SmaI CCC'GGG
Cuts at: 3807 3807
Size: 6785
SmlI C'TyrA_G
Cuts at: 2278 2885 2903 3940 4855 5347 5609 2278
Size: 607 18 1037 915 492 262 3454
Fragments arranged by size:
3454 1037 915 607 492 262 18
SpeI A'CTAG_T
Cuts at: 67 370 67
Size: 303 6482
SphI G_CATG'C
Cuts at: 3838 4338 3838
Size: 500 6285
SspI AAT'ATT
Cuts at: 1327 1728 1327
Size: 401 6384
StuI AGG'CCT
Cuts at: 2848 2848
Size: 6785
StyI C'CwwG_G
Cuts at: 1733 1948 2391 2804 3069 3919 4273 1733
Size: 215 443 413 265 850 354 4245
Fragments arranged by size:
4245 850 443 413 354 265 215
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 4368 4501 4960 5169 4368
Size: 133 459 209 5984
Fragments arranged by size:
5984 459 209 133
TatI w'GTAC_w
Cuts at: 858 1906 2398 2881 3380 6300 858
Size: 1048 492 483 499 2920 1343
Fragments arranged by size:
2920 1343 1048 499 492 483
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1499 1531 3251 3283 6302 6334 1499
Size: 32 1720 32 3019 32 1950
Fragments arranged by size:
3019 1950 1720 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4582 4582
Size: 6785
XbaI T'CTAG_A
Cuts at: 2364 3816 2364
Size: 1452 5333
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4280 4280
Size: 6785
XmaI C'CCGG_G
Cuts at: 3805 3805
Size: 6785
XmnI GAAnn'nnTTC
Cuts at: 976 999 1645 1776 2246 2360 2518 2957 976
Size: 23 646 131 470 114 158 439 4804
Fragments arranged by size:
4804 646 470 439 158 131 114 23
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PciI PflMI PfoI PmeI PpiI PpuMI PsiI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AleI AlfI ApaI AscI AvrII BaeI
BcgI BglI BmgBI BmtI BsiWI BssHII BstEII ClaI
CspCI EcoRV FseI FspAI MfeI NheI NotI NruI
PacI PasI PmlI PshAI PspOMI PspXI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI