Restriction map for
pF1KA0428
(Circular) (Six-base) MAPSORT of: pF1KA0428.seq Check: 5315 from: 1 to: 4223
pF1KA0428 4223 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 1261 1261
Size: 4223
Acc65I G'GTAC_C
Cuts at: 1239 1239
Size: 4223
AclI AA'CG_TT
Cuts at: 4063 4063
Size: 4223
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2048 2480 3227 3495 2048
Size: 432 747 268 2776
Fragments arranged by size:
2776 747 432 268
AflIII A'CryG_T
Cuts at: 1575 2679 3656 1575
Size: 1104 977 2142
Fragments arranged by size:
2142 1104 977
AgeI A'CCGG_T
Cuts at: 2611 2611
Size: 4223
AlwNI CAG_nnn'CTG
Cuts at: 613 622 763 1099 1528 3095 613
Size: 9 141 336 429 1567 1741
Fragments arranged by size:
1741 1567 429 336 141 9
ApaLI G'TGCA_C
Cuts at: 1078 2993 1078
Size: 1915 2308
ApoI r'AATT_y
Cuts at: 211 917 1227 3576 3832 211
Size: 706 310 2349 256 602
Fragments arranged by size:
2349 706 602 310 256
AseI AT'TA_AT
Cuts at: 20 634 20
Size: 614 3609
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4223
AvaI C'yCGr_G
Cuts at: 797 1243 797
Size: 446 3777
BamHI G'GATC_C
Cuts at: 1248 1248
Size: 4223
BanI G'GyrC_C
Cuts at: 433 976 1239 1653 1901 1936 433
Size: 543 263 414 248 35 2720
Fragments arranged by size:
2720 543 414 263 248 35
BanII G_rGCy'C
Cuts at: 1237 2267 1237
Size: 1030 3193
BbeI G_GCGC'C
Cuts at: 1657 1905 1657
Size: 248 3975
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 206 240 206
Size: 34 4189
BciVI GTATCCnnnnn_n'
Cuts at: 2121 2882 4223 2121
Size: 761 1341 2121
Fragments arranged by size:
2121 1341 761
BclI T'GATC_A
Cuts at: 1743 1743
Size: 4223
BfrBI ATG'CAT
Cuts at: 490 490
Size: 4223
BglI GCCn_nnn'nGGC
Cuts at: 394 394
Size: 4223
BglII A'GATC_T
Cuts at: 551 1738 3775 551
Size: 1187 2037 999
Fragments arranged by size:
2037 1187 999
BlpI GC'TnA_GC
Cuts at: 477 1335 477
Size: 858 3365
Bme1580I G_kGCm'C
Cuts at: 438 1082 1848 1941 2997 438
Size: 644 766 93 1056 1664
Fragments arranged by size:
1664 1056 766 644 93
BmrI ACTGGGnnnn_n'
Cuts at: 1435 1516 1851 3995 1435
Size: 81 335 2144 1663
Fragments arranged by size:
2144 1663 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 812 974 2590 812
Size: 162 1616 2445
Fragments arranged by size:
2445 1616 162
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 276 276
Size: 4223
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 673 908 1399 2278 2770 3068 673
Size: 235 491 879 492 298 1828
Fragments arranged by size:
1828 879 492 491 298 235
BsaI GGTCTCn'nnnn_
Cuts at: 3927 3927
Size: 4223
BsaAI yAC'GTr
Cuts at: 1576 1576
Size: 4223
BsaBI GATnn'nnATC
Cuts at: 6 1737 3774 6
Size: 1731 2037 455
Fragments arranged by size:
2037 1731 455
BsaHI Gr'CG_yC
Cuts at: 1654 1902 2604 4100 1654
Size: 248 702 1496 1777
Fragments arranged by size:
1777 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1412 1933 2611 2885 3032 3443 1412
Size: 521 678 274 147 411 2192
Fragments arranged by size:
2192 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 707 707
Size: 4223
BseYI C'CCAG_C
Cuts at: 398 1648 2406 2983 3479 398
Size: 1250 758 577 496 1142
Fragments arranged by size:
1250 1142 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 548 608 812 869 548
Size: 60 204 57 3902
Fragments arranged by size:
3902 204 60 57
BsiEI CG_ry'CG
Cuts at: 104 1765 1811 3019 104
Size: 1661 46 1208 1308
Fragments arranged by size:
1661 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1082 1237 2015 2205 2997 1082
Size: 155 778 190 792 2308
Fragments arranged by size:
2308 792 778 190 155
Bsp1286I G_dGCh'C
Cuts at: 438 1082 1237 1848 1941 2015 2205 2267 2997
Size: 644 155 611 93 74 190 62 730
Cuts at: 2997 438
Size: 1664
Fragments arranged by size:
1664 730 644 611 190 155 93 74
62
BspEI T'CCGG_A
Cuts at: 3443 3443
Size: 4223
BspHI T'CATG_A
Cuts at: 3399 3399
Size: 4223
BspMI ACCTGCnnnn'nnnn_
Cuts at: 612 1273 1487 1789 2170 612
Size: 661 214 302 381 2665
Fragments arranged by size:
2665 661 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2515 2569 4053 4218 50
Size: 2465 54 1484 165 55
Fragments arranged by size:
2465 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2135 2135
Size: 4223
BsrFI r'CCGG_y
Cuts at: 2221 2402 2611 2221
Size: 181 209 3833
Fragments arranged by size:
3833 209 181
BssSI C'ACGA_G
Cuts at: 2494 2852 2494
Size: 358 3865
BstAPI GCAn_nnn'nTGC
Cuts at: 613 622 892 1600 613
Size: 9 270 708 3236
Fragments arranged by size:
3236 708 270 9
BstBI TT'CG_AA
Cuts at: 226 2584 226
Size: 2358 1865
BstYI r'GATC_y
Cuts at: 1 551 1248 1715 1738 2073 2319 3331 3417
Size: 550 697 467 23 335 246 1012 86
Cuts at: 3417 3429 3775 1
Size: 12 346 449
Fragments arranged by size:
1012 697 550 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 911 1073 107
Size: 804 162 3257
Fragments arranged by size:
3257 804 162
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2173 2354 2517 2173
Size: 181 163 3879
Fragments arranged by size:
3879 181 163
BtsI GCAGTG_nn'
Cuts at: 1466 1466
Size: 4223
DraI TTT'AAA
Cuts at: 332 1222 332
Size: 890 3333
DraIII CAC_nnn'GTG
Cuts at: 767 767
Size: 4223
DrdI GACnn_nn'nnGTC
Cuts at: 757 1929 2787 757
Size: 1172 858 2193
Fragments arranged by size:
2193 1172 858
EaeI y'GGCC_r
Cuts at: 193 386 1808 1982 2373 2400 3745 193
Size: 193 1422 174 391 27 1345 671
Fragments arranged by size:
1422 1345 671 391 193 174 27
EagI C'GGCC_G
Cuts at: 1808 1808
Size: 4223
EarI CTCTTCn'nnn_
Cuts at: 2246 2456 2246
Size: 210 4013
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2431 2741 2887 4033 2431
Size: 310 146 1146 2621
Fragments arranged by size:
2621 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1235 1235
Size: 4223
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 276 2048 2480 3227 3495 276
Size: 1772 432 747 268 1004
Fragments arranged by size:
1772 1004 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 520 1362 520
Size: 842 3381
EcoRI G'AATT_C
Cuts at: 917 1227 917
Size: 310 3913
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 949 2242 949
Size: 1293 2930
FspI TGC'GCA
Cuts at: 2004 2004
Size: 4223
HaeII r_GCGC'y
Cuts at: 1657 1905 2927 3906 1657
Size: 248 1022 979 1974
Fragments arranged by size:
1974 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 242 274 2065 2095 2097 2127 242
Size: 32 1791 30 2 30 2338
Fragments arranged by size:
2338 1791 32 30 30 2
HincII GTy'rAC
Cuts at: 870 1262 870
Size: 392 3831
HindIII A'AGCT_T
Cuts at: 474 474
Size: 4223
KasI G'GCGC_C
Cuts at: 1653 1901 1653
Size: 248 3975
KpnI G_GTAC'C
Cuts at: 1243 1243
Size: 4223
MfeI C'AATT_G
Cuts at: 681 681
Size: 4223
MluI A'CGCG_T
Cuts at: 3656 3656
Size: 4223
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 323 2894 3078 323
Size: 2571 184 1468
Fragments arranged by size:
2571 1468 184
MscI TGG'CCA
Cuts at: 388 1984 388
Size: 1596 2627
MslI CAynn'nnrTG
Cuts at: 388 1202 2212 2339 388
Size: 814 1010 127 2272
Fragments arranged by size:
2272 1010 814 127
MspA1I CmG'CkG
Cuts at: 610 619 883 907 1334 1648 2008 3021 3266
Size: 9 264 24 427 314 360 1013 245
Cuts at: 3266 610
Size: 1567
Fragments arranged by size:
1567 1013 427 360 314 264 245 24
9
NaeI GCC'GGC
Cuts at: 2404 2404
Size: 4223
NarI GG'CG_CC
Cuts at: 1654 1902 1654
Size: 248 3975
NcoI C'CATG_G
Cuts at: 107 911 1073 107
Size: 804 162 3257
Fragments arranged by size:
3257 804 162
NgoMIV G'CCGG_C
Cuts at: 2402 2402
Size: 4223
NsiI A_TGCA'T
Cuts at: 492 492
Size: 4223
NspI r_CATG'y
Cuts at: 1035 1276 1776 2683 1035
Size: 241 500 907 2575
Fragments arranged by size:
2575 907 500 241
PciI A'CATG_T
Cuts at: 2679 2679
Size: 4223
PmeI GTTT'AAAC
Cuts at: 1222 1222
Size: 4223
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3362 3394 3362
Size: 32 4191
PpuMI rG'GwC_Cy
Cuts at: 520 520
Size: 4223
PstI C_TGCA'G
Cuts at: 609 618 882 897 1270 1955 609
Size: 9 264 15 373 685 2877
Fragments arranged by size:
2877 685 373 264 15 9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4223
PvuII CAG'CTG
Cuts at: 610 619 883 907 1648 2008 610
Size: 9 264 24 741 360 2825
Fragments arranged by size:
2825 741 360 264 24 9
RsrII CG'GwC_CG
Cuts at: 2418 2418
Size: 4223
SacI G_AGCT'C
Cuts at: 1237 1237
Size: 4223
SalI G'TCGA_C
Cuts at: 1260 1260
Size: 4223
SapI GCTCTTCn'nnn_
Cuts at: 2246 2456 2246
Size: 210 4013
SbfI CC_TGCA'GG
Cuts at: 1270 1270
Size: 4223
ScaI AGT'ACT
Cuts at: 3740 3740
Size: 4223
SexAI A'CCwGG_T
Cuts at: 863 863
Size: 4223
SfcI C'TryA_G
Cuts at: 33 605 614 878 893 1266 1951 2944 3135
Size: 572 9 264 15 373 685 993 191
Cuts at: 3135 33
Size: 1121
Fragments arranged by size:
1121 993 685 572 373 264 191 15
9
SfiI GGCCn_nnn'nGGCC
Cuts at: 394 394
Size: 4223
SfoI GGC'GCC
Cuts at: 1655 1903 1655
Size: 248 3975
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4223
SmaI CCC'GGG
Cuts at: 1245 1245
Size: 4223
SmlI C'TyrA_G
Cuts at: 652 923 1378 2293 2785 3047 652
Size: 271 455 915 492 262 1828
Fragments arranged by size:
1828 915 492 455 271 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4223
SphI G_CATG'C
Cuts at: 1276 1776 1276
Size: 500 3723
StuI AGG'CCT
Cuts at: 954 954
Size: 4223
StyI C'CwwG_G
Cuts at: 107 911 1073 1357 1711 107
Size: 804 162 284 354 2619
Fragments arranged by size:
2619 804 354 284 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1806 1939 2398 2607 1806
Size: 133 459 209 3422
Fragments arranged by size:
3422 459 209 133
TatI w'GTAC_w
Cuts at: 930 3738 930
Size: 2808 1415
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 576 608 1116 1148 3740 3772 576
Size: 32 508 32 2592 32 1027
Fragments arranged by size:
2592 1027 508 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2020 2020
Size: 4223
XbaI T'CTAG_A
Cuts at: 1254 1254
Size: 4223
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 244 1718 244
Size: 1474 2749
XmaI C'CCGG_G
Cuts at: 1243 1243
Size: 4223
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PciI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BmgBI BmtI
Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI
FspAI HpaI NdeI NheI NotI NruI PacI PasI
PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI