Restriction map for
pF1KA0431
(Circular) (Six-base) MAPSORT of: pF1KA0431.seq Check: 5880 from: 1 to: 5114
pF1KA0431 5114 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 2152 2152
Size: 5114
Acc65I G'GTAC_C
Cuts at: 2130 2130
Size: 5114
AclI AA'CG_TT
Cuts at: 4954 4954
Size: 5114
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 410 1613 1886 2939 3371 4118 4386 410
Size: 1203 273 1053 432 747 268 1138
Fragments arranged by size:
1203 1138 1053 747 432 273 268
AflII C'TTAA_G
Cuts at: 724 724
Size: 5114
AflIII A'CryG_T
Cuts at: 842 844 2466 3570 4547 842
Size: 2 1622 1104 977 1409
Fragments arranged by size:
1622 1409 1104 977 2
AgeI A'CCGG_T
Cuts at: 3502 3502
Size: 5114
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2038 2070 2038
Size: 32 5082
AlwNI CAG_nnn'CTG
Cuts at: 658 2419 3986 658
Size: 1761 1567 1786
Fragments arranged by size:
1786 1761 1567
ApaLI G'TGCA_C
Cuts at: 216 338 661 1346 3884 216
Size: 122 323 685 2538 1446
Fragments arranged by size:
2538 1446 685 323 122
ApoI r'AATT_y
Cuts at: 1338 1710 2118 4467 4723 1338
Size: 372 408 2349 256 1729
Fragments arranged by size:
2349 1729 408 372 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5114
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5114
AvaI C'yCGr_G
Cuts at: 2134 2134
Size: 5114
BamHI G'GATC_C
Cuts at: 2139 2139
Size: 5114
BanI G'GyrC_C
Cuts at: 2130 2544 2792 2827 2130
Size: 414 248 35 4417
Fragments arranged by size:
4417 414 248 35
BanII G_rGCy'C
Cuts at: 646 2128 3158 646
Size: 1482 1030 2602
Fragments arranged by size:
2602 1482 1030
BbeI G_GCGC'C
Cuts at: 2548 2796 2548
Size: 248 4866
BbsI GAAGACnn'nnnn_
Cuts at: 461 461
Size: 5114
BbvCI CC'TCA_GC
Cuts at: 609 609
Size: 5114
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 271 305 271
Size: 34 5080
BciVI GTATCCnnnnn_n'
Cuts at: 3012 3773 5114 3012
Size: 761 1341 3012
Fragments arranged by size:
3012 1341 761
BclI T'GATC_A
Cuts at: 636 2634 636
Size: 1998 3116
BfrBI ATG'CAT
Cuts at: 117 1072 117
Size: 955 4159
BglI GCCn_nnn'nGGC
Cuts at: 655 655
Size: 5114
BglII A'GATC_T
Cuts at: 885 2629 4666 885
Size: 1744 2037 1333
Fragments arranged by size:
2037 1744 1333
BlpI GC'TnA_GC
Cuts at: 2226 2226
Size: 5114
Bme1580I G_kGCm'C
Cuts at: 220 281 342 665 1350 2739 2832 3888 220
Size: 61 61 323 685 1389 93 1056 1446
Fragments arranged by size:
1446 1389 1056 685 323 93 61 61
BmrI ACTGGGnnnn_n'
Cuts at: 284 2326 2407 2742 4886 284
Size: 2042 81 335 2144 512
Fragments arranged by size:
2144 2042 512 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 527 735 755 3481 527
Size: 208 20 2726 2160
Fragments arranged by size:
2726 2160 208 20
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1092 1803 1092
Size: 711 4403
Bpu10I CC'TnA_GC
Cuts at: 609 609
Size: 5114
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 395 971 1750 2290 3169 3661 3959 395
Size: 576 779 540 879 492 298 1550
Fragments arranged by size:
1550 879 779 576 540 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1110 1849 4818 1110
Size: 739 2969 1406
Fragments arranged by size:
2969 1406 739
BsaAI yAC'GTr
Cuts at: 845 2467 845
Size: 1622 3492
BsaBI GATnn'nnATC
Cuts at: 6 2628 4665 6
Size: 2622 2037 455
Fragments arranged by size:
2622 2037 455
BsaHI Gr'CG_yC
Cuts at: 2545 2793 3495 4991 2545
Size: 248 702 1496 2668
Fragments arranged by size:
2668 1496 702 248
BsaWI w'CCGG_w
Cuts at: 212 2303 2824 3502 3776 3923 4334 212
Size: 2091 521 678 274 147 411 992
Fragments arranged by size:
2091 992 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2038 2068 2083 2113 2038
Size: 30 15 30 5039
Fragments arranged by size:
5039 30 30 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 597 597
Size: 5114
BseYI C'CCAG_C
Cuts at: 614 1030 1505 1521 2539 3297 3874 4370 614
Size: 416 475 16 1018 758 577 496 1358
Fragments arranged by size:
1358 1018 758 577 496 475 416 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 168 898 905 1310 168
Size: 730 7 405 3972
Fragments arranged by size:
3972 730 405 7
BsiEI CG_ry'CG
Cuts at: 104 2656 2702 3910 104
Size: 2552 46 1208 1308
Fragments arranged by size:
2552 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 220 342 665 1350 2128 2906 3096 3888 220
Size: 122 323 685 778 778 190 792 1446
Fragments arranged by size:
1446 792 778 778 685 323 190 122
BsiWI C'GTAC_G
Cuts at: 158 158
Size: 5114
Bsp1286I G_dGCh'C
Cuts at: 220 281 342 646 665 1350 2128 2739 2832
Size: 61 61 304 19 685 778 611 93
Cuts at: 2832 2906 3096 3158 3888 220
Size: 74 190 62 730 1446
Fragments arranged by size:
1446 778 730 685 611 304 190 93
74 62 61 61 19
BspEI T'CCGG_A
Cuts at: 4334 4334
Size: 5114
BspHI T'CATG_A
Cuts at: 4290 4290
Size: 5114
BspMI ACCTGCnnnn'nnnn_
Cuts at: 297 1902 2164 2378 2680 3061 297
Size: 1605 262 214 302 381 2350
Fragments arranged by size:
2350 1605 381 302 262 214
BsrBI CCG'CTC
Cuts at: 50 3406 3460 4944 5109 50
Size: 3356 54 1484 165 55
Fragments arranged by size:
3356 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2009 3026 2009
Size: 1017 4097
BsrFI r'CCGG_y
Cuts at: 3112 3293 3502 3112
Size: 181 209 4724
Fragments arranged by size:
4724 209 181
BsrGI T'GTAC_A
Cuts at: 1155 1155
Size: 5114
BssSI C'ACGA_G
Cuts at: 280 3385 3743 280
Size: 3105 358 1651
Fragments arranged by size:
3105 1651 358
BstAPI GCAn_nnn'nTGC
Cuts at: 669 2491 669
Size: 1822 3292
BstBI TT'CG_AA
Cuts at: 3475 3475
Size: 5114
BstXI CCAn_nnnn'nTGG
Cuts at: 1099 1099
Size: 5114
BstYI r'GATC_y
Cuts at: 1 885 2139 2606 2629 2964 3210 4222 4308
Size: 884 1254 467 23 335 246 1012 86
Cuts at: 4308 4320 4666 1
Size: 12 346 449
Fragments arranged by size:
1254 1012 884 467 449 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3064 3245 3408 3064
Size: 181 163 4770
Fragments arranged by size:
4770 181 163
BtsI GCAGTG_nn'
Cuts at: 250 932 1877 2357 250
Size: 682 945 480 3007
Fragments arranged by size:
3007 945 682 480
ClaI AT'CG_AT
Cuts at: 1541 1541
Size: 5114
DraI TTT'AAA
Cuts at: 1304 2113 1304
Size: 809 4305
DrdI GACnn_nn'nnGTC
Cuts at: 888 2820 3678 888
Size: 1932 858 2324
Fragments arranged by size:
2324 1932 858
EaeI y'GGCC_r
Cuts at: 2699 2873 3264 3291 4636 2699
Size: 174 391 27 1345 3177
Fragments arranged by size:
3177 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2699 2699
Size: 5114
EarI CTCTTCn'nnn_
Cuts at: 1001 1550 1589 3137 3347 1001
Size: 549 39 1548 210 2768
Fragments arranged by size:
2768 1548 549 210 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3322 3632 3778 4924 3322
Size: 310 146 1146 3512
Fragments arranged by size:
3512 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2126 2126
Size: 5114
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 410 1092 1613 1803 1886 2939 3371 4118 4386
Size: 682 521 190 83 1053 432 747 268
Cuts at: 4386 410
Size: 1138
Fragments arranged by size:
1138 1053 747 682 521 432 268 190
83
EcoO109I rG'GnC_Cy
Cuts at: 174 303 2253 174
Size: 129 1950 3035
Fragments arranged by size:
3035 1950 129
EcoRI G'AATT_C
Cuts at: 2118 2118
Size: 5114
EcoRV GAT'ATC
Cuts at: 508 1551 508
Size: 1043 4071
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1012 3133 1012
Size: 2121 2993
FspI TGC'GCA
Cuts at: 2895 2895
Size: 5114
HaeII r_GCGC'y
Cuts at: 2548 2796 3818 4797 2548
Size: 248 1022 979 2865
Fragments arranged by size:
2865 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 628 660 1122 1154 1251 1283 1290 1322 1371
Size: 32 462 32 97 32 7 32 49
Cuts at: 1371 1403 1446 1478 2034 2066 2956 2986 2988
Size: 32 43 32 556 32 890 30 2
Cuts at: 2988 3018 628
Size: 30 2724
Fragments arranged by size:
2724 890 556 462 97 49 43 32
32 32 32 32 32 32 30 30
7 2
HincII GTy'rAC
Cuts at: 2153 2153
Size: 5114
HindIII A'AGCT_T
Cuts at: 422 422
Size: 5114
KasI G'GCGC_C
Cuts at: 2544 2792 2544
Size: 248 4866
KpnI G_GTAC'C
Cuts at: 2134 2134
Size: 5114
MluI A'CGCG_T
Cuts at: 4547 4547
Size: 5114
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 383 1165 2126 3785 3969 383
Size: 782 961 1659 184 1528
Fragments arranged by size:
1659 1528 961 782 184
MscI TGG'CCA
Cuts at: 2875 2875
Size: 5114
MslI CAynn'nnrTG
Cuts at: 112 3103 3230 112
Size: 2991 127 1996
Fragments arranged by size:
2991 1996 127
MspA1I CmG'CkG
Cuts at: 2225 2539 2899 3912 4157 2225
Size: 314 360 1013 245 3182
Fragments arranged by size:
3182 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3295 3295
Size: 5114
NarI GG'CG_CC
Cuts at: 2545 2793 2545
Size: 248 4866
NgoMIV G'CCGG_C
Cuts at: 3293 3293
Size: 5114
NsiI A_TGCA'T
Cuts at: 119 1074 119
Size: 955 4159
NspI r_CATG'y
Cuts at: 121 190 224 2167 2667 3574 121
Size: 69 34 1943 500 907 1661
Fragments arranged by size:
1943 1661 907 500 69 34
PasI CC'CwG_GG
Cuts at: 1962 1962
Size: 5114
PciI A'CATG_T
Cuts at: 3570 3570
Size: 5114
PflMI CCAn_nnn'nTGG
Cuts at: 621 1458 621
Size: 837 4277
PfoI T'CCnGG_A
Cuts at: 1491 1491
Size: 5114
PmeI GTTT'AAAC
Cuts at: 2113 2113
Size: 5114
PmlI CAC'GTG
Cuts at: 845 845
Size: 5114
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2038 2070 4253 4285 2038
Size: 32 2183 32 2867
Fragments arranged by size:
2867 2183 32 32
PpuMI rG'GwC_Cy
Cuts at: 174 303 174
Size: 129 4985
PsiI TTA'TAA
Cuts at: 1237 1336 1237
Size: 99 5015
PstI C_TGCA'G
Cuts at: 257 294 2161 2846 257
Size: 37 1867 685 2525
Fragments arranged by size:
2525 1867 685 37
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5114
PvuII CAG'CTG
Cuts at: 2539 2899 2539
Size: 360 4754
RsrII CG'GwC_CG
Cuts at: 3309 3309
Size: 5114
SacI G_AGCT'C
Cuts at: 2128 2128
Size: 5114
SalI G'TCGA_C
Cuts at: 2151 2151
Size: 5114
SanDI GG'GwC_CC
Cuts at: 303 303
Size: 5114
SapI GCTCTTCn'nnn_
Cuts at: 3137 3347 3137
Size: 210 4904
SbfI CC_TGCA'GG
Cuts at: 2161 2161
Size: 5114
ScaI AGT'ACT
Cuts at: 783 1836 4631 783
Size: 1053 2795 1266
Fragments arranged by size:
2795 1266 1053
SfcI C'TryA_G
Cuts at: 33 253 290 596 1052 1223 2063 2157 2842
Size: 220 37 306 456 171 840 94 685
Cuts at: 2842 3835 4026 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 840 685 456 306 220 191
171 94 37
SfoI GGC'GCC
Cuts at: 2546 2794 2546
Size: 248 4866
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5114
SmaI CCC'GGG
Cuts at: 2136 2136
Size: 5114
SmlI C'TyrA_G
Cuts at: 410 724 986 1729 2269 3184 3676 3938 410
Size: 314 262 743 540 915 492 262 1586
Fragments arranged by size:
1586 915 743 540 492 314 262 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5114
SphI G_CATG'C
Cuts at: 121 2167 2667 121
Size: 2046 500 2568
Fragments arranged by size:
2568 2046 500
StuI AGG'CCT
Cuts at: 1727 1727
Size: 5114
StyI C'CwwG_G
Cuts at: 2248 2602 2248
Size: 354 4760
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1519 1688 2697 2830 3289 3498 1519
Size: 169 1009 133 459 209 3135
Fragments arranged by size:
3135 1009 459 209 169 133
TatI w'GTAC_w
Cuts at: 781 1155 1834 1924 4629 781
Size: 374 679 90 2705 1266
Fragments arranged by size:
2705 1266 679 374 90
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2083 2115 4631 4663 2083
Size: 32 2516 32 2534
Fragments arranged by size:
2534 2516 32 32
Tth111I GACn'n_nGTC
Cuts at: 2911 2911
Size: 5114
XbaI T'CTAG_A
Cuts at: 1899 2145 1899
Size: 246 4868
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 543 1513 2609 543
Size: 970 1096 3048
Fragments arranged by size:
3048 1096 970
XmaI C'CCGG_G
Cuts at: 2134 2134
Size: 5114
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PsiI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI ApaI AscI
AvrII BaeI BmgBI BmtI BsmI BsmBI BssHII BstEII
BstZ17I Bsu36I BtgI CspCI DraIII EcoNI FseI FspAI
HpaI MfeI NcoI NdeI NheI NotI NruI PacI
PshAI PspOMI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI