Restriction map for
pF1KA0439
(Circular) (Six-base) MAPSORT of: pF1KA0439.seq Check: 2306 from: 1 to: 5615
pF1KA0439 5615 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 2653 2653
Size: 5615
Acc65I G'GTAC_C
Cuts at: 2631 2631
Size: 5615
AclI AA'CG_TT
Cuts at: 1375 5455 1375
Size: 4080 1535
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 673 833 1479 1747 3440 3872 4619 4887 673
Size: 160 646 268 1693 432 747 268 1401
Fragments arranged by size:
1693 1401 747 646 432 268 268 160
AflIII A'CryG_T
Cuts at: 428 2967 4071 5048 428
Size: 2539 1104 977 995
Fragments arranged by size:
2539 1104 995 977
AgeI A'CCGG_T
Cuts at: 4003 4003
Size: 5615
AhdI GACnn_n'nnGTC
Cuts at: 1609 1609
Size: 5615
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1990 2022 1990
Size: 32 5583
AlwNI CAG_nnn'CTG
Cuts at: 663 2920 4487 663
Size: 2257 1567 1791
Fragments arranged by size:
2257 1791 1567
ApaLI G'TGCA_C
Cuts at: 4385 4385
Size: 5615
ApoI r'AATT_y
Cuts at: 1276 1355 1471 2207 2619 4968 5224 1276
Size: 79 116 736 412 2349 256 1667
Fragments arranged by size:
2349 1667 736 412 256 116 79
AseI AT'TA_AT
Cuts at: 20 20
Size: 5615
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5615
AvaI C'yCGr_G
Cuts at: 164 334 526 553 2635 164
Size: 170 192 27 2082 3144
Fragments arranged by size:
3144 2082 192 170 27
BamHI G'GATC_C
Cuts at: 1970 2640 1970
Size: 670 4945
BanI G'GyrC_C
Cuts at: 1063 2631 3045 3293 3328 1063
Size: 1568 414 248 35 3350
Fragments arranged by size:
3350 1568 414 248 35
BanII G_rGCy'C
Cuts at: 527 533 2502 2629 3659 527
Size: 6 1969 127 1030 2483
Fragments arranged by size:
2483 1969 1030 127 6
BbeI G_GCGC'C
Cuts at: 3049 3297 3049
Size: 248 5367
BbsI GAAGACnn'nnnn_
Cuts at: 521 1421 521
Size: 900 4715
BbvCI CC'TCA_GC
Cuts at: 1024 2450 1024
Size: 1426 4189
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 766 800 766
Size: 34 5581
BciVI GTATCCnnnnn_n'
Cuts at: 2380 3513 4274 5615 2380
Size: 1133 761 1341 2380
Fragments arranged by size:
2380 1341 1133 761
BclI T'GATC_A
Cuts at: 1233 1386 3135 1233
Size: 153 1749 3713
Fragments arranged by size:
3713 1749 153
BglII A'GATC_T
Cuts at: 3130 5167 3130
Size: 2037 3578
BlpI GC'TnA_GC
Cuts at: 2727 2727
Size: 5615
Bme1580I G_kGCm'C
Cuts at: 3240 3333 4389 3240
Size: 93 1056 4466
Fragments arranged by size:
4466 1056 93
BmgBI CAC'GTC
Cuts at: 429 429
Size: 5615
BmrI ACTGGGnnnn_n'
Cuts at: 2827 2908 3243 5387 2827
Size: 81 335 2144 3055
Fragments arranged by size:
3055 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 619 3982 619
Size: 3363 2252
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 614 683 1078 1449 1996 2248 2292 614
Size: 69 395 371 547 252 44 3937
Fragments arranged by size:
3937 547 395 371 252 69 44
Bpu10I CC'TnA_GC
Cuts at: 875 1024 2450 875
Size: 149 1426 4040
Fragments arranged by size:
4040 1426 149
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 735 2573 2791 3670 4162 4460 735
Size: 1838 218 879 492 298 1890
Fragments arranged by size:
1890 1838 879 492 298 218
BsaI GGTCTCn'nnnn_
Cuts at: 559 5319 559
Size: 4760 855
BsaAI yAC'GTr
Cuts at: 2968 2968
Size: 5615
BsaBI GATnn'nnATC
Cuts at: 6 3129 5166 6
Size: 3123 2037 455
Fragments arranged by size:
3123 2037 455
BsaHI Gr'CG_yC
Cuts at: 2357 3046 3294 3996 5492 2357
Size: 689 248 702 1496 2480
Fragments arranged by size:
2480 1496 702 689 248
BsaWI w'CCGG_w
Cuts at: 392 961 2372 2804 3325 4003 4277 4424 4835
Size: 569 1411 432 521 678 274 147 411
Cuts at: 4835 392
Size: 1172
Fragments arranged by size:
1411 1172 678 569 521 432 411 274
147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 130 308 311 314 738 130
Size: 178 3 3 424 5007
Fragments arranged by size:
5007 424 178 3 3
BseYI C'CCAG_C
Cuts at: 854 1190 1343 1434 3040 3798 4375 4871 854
Size: 336 153 91 1606 758 577 496 1598
Fragments arranged by size:
1606 1598 758 577 496 336 153 91
BsiEI CG_ry'CG
Cuts at: 104 1218 3157 3203 4411 104
Size: 1114 1939 46 1208 1308
Fragments arranged by size:
1939 1308 1208 1114 46
BsiHKAI G_wGCw'C
Cuts at: 2502 2629 3407 3597 4389 2502
Size: 127 778 190 792 3728
Fragments arranged by size:
3728 792 778 190 127
BsiWI C'GTAC_G
Cuts at: 1080 1080
Size: 5615
BsmI GAATG_Cn'
Cuts at: 2280 2280
Size: 5615
Bsp1286I G_dGCh'C
Cuts at: 527 533 2502 2629 3240 3333 3407 3597 3659
Size: 6 1969 127 611 93 74 190 62
Cuts at: 3659 4389 527
Size: 730 1753
Fragments arranged by size:
1969 1753 730 611 190 127 93 74
62 6
BspEI T'CCGG_A
Cuts at: 961 4835 961
Size: 3874 1741
BspHI T'CATG_A
Cuts at: 4791 4791
Size: 5615
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1524 2665 2879 3181 3562 1524
Size: 1141 214 302 381 3577
Fragments arranged by size:
3577 1141 381 302 214
BsrBI CCG'CTC
Cuts at: 50 494 3907 3961 5445 5610 50
Size: 444 3413 54 1484 165 55
Fragments arranged by size:
3413 1484 444 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2476 3527 2476
Size: 1051 4564
BsrFI r'CCGG_y
Cuts at: 482 627 1010 1217 3613 3794 4003 482
Size: 145 383 207 2396 181 209 2094
Fragments arranged by size:
2396 2094 383 209 207 181 145
BsrGI T'GTAC_A
Cuts at: 825 825
Size: 5615
BssSI C'ACGA_G
Cuts at: 3886 4244 3886
Size: 358 5257
BstAPI GCAn_nnn'nTGC
Cuts at: 2992 2992
Size: 5615
BstBI TT'CG_AA
Cuts at: 3976 3976
Size: 5615
BstXI CCAn_nnnn'nTGG
Cuts at: 1190 1190
Size: 5615
BstYI r'GATC_y
Cuts at: 1 640 1004 1263 1970 2640 3107 3130 3465
Size: 639 364 259 707 670 467 23 335
Cuts at: 3465 3711 4723 4809 4821 5167 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 707 670 639 467 449 364 346
335 259 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 656 656
Size: 5615
BtgI C'CryG_G
Cuts at: 107 1745 2576 107
Size: 1638 831 3146
Fragments arranged by size:
3146 1638 831
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2384 3565 3746 3909 2384
Size: 1181 181 163 4090
Fragments arranged by size:
4090 1181 181 163
BtsI GCAGTG_nn'
Cuts at: 774 2858 774
Size: 2084 3531
DraI TTT'AAA
Cuts at: 1310 2614 1310
Size: 1304 4311
DraIII CAC_nnn'GTG
Cuts at: 404 404
Size: 5615
DrdI GACnn_nn'nnGTC
Cuts at: 152 3321 4179 152
Size: 3169 858 1588
Fragments arranged by size:
3169 1588 858
EaeI y'GGCC_r
Cuts at: 1215 1368 1673 1841 2573 3200 3374 3765 3792
Size: 153 305 168 732 627 174 391 27
Cuts at: 3792 5137 1215
Size: 1345 1693
Fragments arranged by size:
1693 1345 732 627 391 305 174 168
153 27
EagI C'GGCC_G
Cuts at: 1215 3200 1215
Size: 1985 3630
EarI CTCTTCn'nnn_
Cuts at: 1565 1821 3638 3848 1565
Size: 256 1817 210 3332
Fragments arranged by size:
3332 1817 256 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 997 3823 4133 4279 5425 997
Size: 2826 310 146 1146 1187
Fragments arranged by size:
2826 1187 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2500 2627 2500
Size: 127 5488
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 614 673 683 833 1078 1449 1479 1747 1996
Size: 59 10 150 245 371 30 268 249
Cuts at: 1996 2248 2292 3440 3872 4619 4887 614
Size: 252 44 1148 432 747 268 1342
Fragments arranged by size:
1342 1148 747 432 371 268 268 252
249 245 150 59 44 30 10
EcoNI CCTnn'n_nnAGG
Cuts at: 750 1778 750
Size: 1028 4587
EcoO109I rG'GnC_Cy
Cuts at: 2754 2754
Size: 5615
EcoRI G'AATT_C
Cuts at: 1355 2619 1355
Size: 1264 4351
EcoRV GAT'ATC
Cuts at: 1524 1524
Size: 5615
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 328 1120 3634 328
Size: 792 2514 2309
Fragments arranged by size:
2514 2309 792
FseI GG_CCGG'CC
Cuts at: 1221 1221
Size: 5615
FspI TGC'GCA
Cuts at: 3396 3396
Size: 5615
HaeII r_GCGC'y
Cuts at: 489 1210 3049 3297 4319 5298 489
Size: 721 1839 248 1022 979 806
Fragments arranged by size:
1839 1022 979 806 721 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 418 450 639 671 943 975 1595 1627 1659
Size: 32 189 32 272 32 620 32 32
Cuts at: 1659 1789 1821 1918 1950 3457 3487 3489 3519
Size: 130 32 97 32 1507 30 2 30
Cuts at: 3519 418
Size: 2514
Fragments arranged by size:
2514 1507 620 272 189 130 97 32
32 32 32 32 32 32 30 30
2
HincII GTy'rAC
Cuts at: 276 381 2654 276
Size: 105 2273 3237
Fragments arranged by size:
3237 2273 105
KasI G'GCGC_C
Cuts at: 3045 3293 3045
Size: 248 5367
KpnI G_GTAC'C
Cuts at: 2635 2635
Size: 5615
MluI A'CGCG_T
Cuts at: 5048 5048
Size: 5615
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 422 1133 4286 4470 422
Size: 711 3153 184 1567
Fragments arranged by size:
3153 1567 711 184
MscI TGG'CCA
Cuts at: 1675 3376 1675
Size: 1701 3914
MslI CAynn'nnrTG
Cuts at: 1364 2575 3604 3731 1364
Size: 1211 1029 127 3248
Fragments arranged by size:
3248 1211 1029 127
MspA1I CmG'CkG
Cuts at: 1056 2454 2726 3040 3400 4413 4658 1056
Size: 1398 272 314 360 1013 245 2013
Fragments arranged by size:
2013 1398 1013 360 314 272 245
NaeI GCC'GGC
Cuts at: 1012 1219 3796 1012
Size: 207 2577 2831
Fragments arranged by size:
2831 2577 207
NarI GG'CG_CC
Cuts at: 3046 3294 3046
Size: 248 5367
NcoI C'CATG_G
Cuts at: 107 107
Size: 5615
NdeI CA'TA_TG
Cuts at: 1289 2535 1289
Size: 1246 4369
NgoMIV G'CCGG_C
Cuts at: 1010 1217 3794 1010
Size: 207 2577 2831
Fragments arranged by size:
2831 2577 207
NruI TCG'CGA
Cuts at: 2403 2403
Size: 5615
NspI r_CATG'y
Cuts at: 2510 2668 3168 4075 2510
Size: 158 500 907 4050
Fragments arranged by size:
4050 907 500 158
PciI A'CATG_T
Cuts at: 4071 4071
Size: 5615
PflMI CCAn_nnn'nTGG
Cuts at: 211 404 920 1682 211
Size: 193 516 762 4144
Fragments arranged by size:
4144 762 516 193
PfoI T'CCnGG_A
Cuts at: 1973 1973
Size: 5615
PmeI GTTT'AAAC
Cuts at: 2614 2614
Size: 5615
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4754 4786 4754
Size: 32 5583
PstI C_TGCA'G
Cuts at: 1430 2384 2662 3347 1430
Size: 954 278 685 3698
Fragments arranged by size:
3698 954 685 278
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5615
PvuII CAG'CTG
Cuts at: 2454 3040 3400 2454
Size: 586 360 4669
Fragments arranged by size:
4669 586 360
RsrII CG'GwC_CG
Cuts at: 3810 3810
Size: 5615
SacI G_AGCT'C
Cuts at: 2502 2629 2502
Size: 127 5488
SalI G'TCGA_C
Cuts at: 2652 2652
Size: 5615
SapI GCTCTTCn'nnn_
Cuts at: 3638 3848 3638
Size: 210 5405
SbfI CC_TGCA'GG
Cuts at: 2662 2662
Size: 5615
ScaI AGT'ACT
Cuts at: 1738 5132 1738
Size: 3394 2221
SfcI C'TryA_G
Cuts at: 33 1426 2380 2658 3343 4336 4527 33
Size: 1393 954 278 685 993 191 1121
Fragments arranged by size:
1393 1121 993 954 685 278 191
SfoI GGC'GCC
Cuts at: 3047 3295 3047
Size: 248 5367
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5615
SgrAI Cr'CCGG_yG
Cuts at: 482 482
Size: 5615
SmaI CCC'GGG
Cuts at: 336 2637 336
Size: 2301 3314
SmlI C'TyrA_G
Cuts at: 164 750 2588 2770 3685 4177 4439 164
Size: 586 1838 182 915 492 262 1340
Fragments arranged by size:
1838 1340 915 586 492 262 182
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5615
SphI G_CATG'C
Cuts at: 2668 3168 2668
Size: 500 5115
SspI AAT'ATT
Cuts at: 1951 1951
Size: 5615
StuI AGG'CCT
Cuts at: 1784 1784
Size: 5615
StyI C'CwwG_G
Cuts at: 107 559 1067 1323 2749 3103 107
Size: 452 508 256 1426 354 2619
Fragments arranged by size:
2619 1426 508 452 354 256
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 595 3198 3331 3790 3999 595
Size: 2603 133 459 209 2211
Fragments arranged by size:
2603 2211 459 209 133
TatI w'GTAC_w
Cuts at: 825 1284 1736 5130 825
Size: 459 452 3394 1310
Fragments arranged by size:
3394 1310 459 452
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5132 5164 5132
Size: 32 5583
Tth111I GACn'n_nGTC
Cuts at: 2113 3412 2113
Size: 1299 4316
XbaI T'CTAG_A
Cuts at: 2646 2646
Size: 5615
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3110 3110
Size: 5615
XhoI C'TCGA_G
Cuts at: 164 164
Size: 5615
XmaI C'CCGG_G
Cuts at: 334 2635 334
Size: 2301 3314
XmnI GAAnn'nnTTC
Cuts at: 1355 2161 2186 1355
Size: 806 25 4784
Fragments arranged by size:
4784 806 25
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NruI NspI PciI PflMI PfoI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI ApaI AscI
AvrII BaeI BfrBI BglI BmtI BsaXI BsgI BsmBI
BssHII BstEII BstZ17I ClaI CspCI FspAI HindIII HpaI
MfeI NheI NotI NsiI PacI PasI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SnaBI SrfI SwaI ZraI