Restriction map for
pF1KA0440
(Circular) (Six-base) MAPSORT of: pF1KA0440.seq Check: 4682 from: 1 to: 8522
pF1KA0440 8522 bp
With 182 enzymes: SgfI *
September 18, 2009 16:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5426 5426
Size: 8522
AatII G_ACGT'C
Cuts at: 4946 4946
Size: 8522
AccI GT'mk_AC
Cuts at: 1453 1780 5560 1453
Size: 327 3780 4415
Fragments arranged by size:
4415 3780 327
Acc65I G'GTAC_C
Cuts at: 5538 5538
Size: 8522
AclI AA'CG_TT
Cuts at: 8362 8362
Size: 8522
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 596 2000 2723 4332 4935 5368 5383 5494 6347
Size: 1404 723 1609 603 433 15 111 853
Cuts at: 6347 6779 7526 7794 596
Size: 432 747 268 1324
Fragments arranged by size:
1609 1404 1324 853 747 723 603 433
432 268 111 15
AflIII A'CryG_T
Cuts at: 1084 1557 5874 6978 7955 1084
Size: 473 4317 1104 977 1651
Fragments arranged by size:
4317 1651 1104 977 473
AgeI A'CCGG_T
Cuts at: 6910 6910
Size: 8522
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 541 573 2036 2068 541
Size: 32 1463 32 6995
Fragments arranged by size:
6995 1463 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1079 1651 1863 2208 2354 5268 5418 5827 7394
Size: 572 212 345 146 2914 150 409 1567
Cuts at: 7394 1079
Size: 2207
Fragments arranged by size:
2914 2207 1567 572 409 345 212 150
146
ApaLI G'TGCA_C
Cuts at: 2329 7292 2329
Size: 4963 3559
ApoI r'AATT_y
Cuts at: 1284 1410 1588 2026 3277 5526 7875 8131 1284
Size: 126 178 438 1251 2249 2349 256 1675
Fragments arranged by size:
2349 2249 1675 1251 438 256 178 126
AseI AT'TA_AT
Cuts at: 20 2474 3886 20
Size: 2454 1412 4656
Fragments arranged by size:
4656 2454 1412
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8522
AvaI C'yCGr_G
Cuts at: 594 3796 3951 5542 594
Size: 3202 155 1591 3574
Fragments arranged by size:
3574 3202 1591 155
AvrII C'CTAG_G
Cuts at: 4516 4516
Size: 8522
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4362 4395 4362
Size: 33 8489
BamHI G'GATC_C
Cuts at: 3897 5547 3897
Size: 1650 6872
BanI G'GyrC_C
Cuts at: 181 3464 4069 4185 5538 5952 6200 6235 181
Size: 3283 605 116 1353 414 248 35 2468
Fragments arranged by size:
3283 2468 1353 605 414 248 116 35
BanII G_rGCy'C
Cuts at: 1739 1850 2666 3044 3874 4390 5536 6566 1739
Size: 111 816 378 830 516 1146 1030 3695
Fragments arranged by size:
3695 1146 1030 830 816 516 378 111
BbeI G_GCGC'C
Cuts at: 3468 5956 6204 3468
Size: 2488 248 5786
Fragments arranged by size:
5786 2488 248
BbsI GAAGACnn'nnnn_
Cuts at: 1070 2711 2787 3560 4268 5390 5487 1070
Size: 1641 76 773 708 1122 97 4105
Fragments arranged by size:
4105 1641 1122 773 708 97 76
BbvCI CC'TCA_GC
Cuts at: 1300 3070 1300
Size: 1770 6752
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3064 3098 3064
Size: 34 8488
BciVI GTATCCnnnnn_n'
Cuts at: 6420 7181 8522 6420
Size: 761 1341 6420
Fragments arranged by size:
6420 1341 761
BclI T'GATC_A
Cuts at: 5247 6042 5247
Size: 795 7727
BglI GCCn_nnn'nGGC
Cuts at: 3061 3717 4313 3061
Size: 656 596 7270
Fragments arranged by size:
7270 656 596
BglII A'GATC_T
Cuts at: 1041 1563 2550 3095 4725 6037 8074 1041
Size: 522 987 545 1630 1312 2037 1489
Fragments arranged by size:
2037 1630 1489 1312 987 545 522
BlpI GC'TnA_GC
Cuts at: 2665 5634 2665
Size: 2969 5553
Bme1580I G_kGCm'C
Cuts at: 1505 2333 3601 3999 4428 6147 6240 7296 1505
Size: 828 1268 398 429 1719 93 1056 2731
Fragments arranged by size:
2731 1719 1268 1056 828 429 398 93
BmrI ACTGGGnnnn_n'
Cuts at: 3534 3672 3869 4402 5734 5815 6150 8294 3534
Size: 138 197 533 1332 81 335 2144 3762
Fragments arranged by size:
3762 2144 1332 533 335 197 138 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 620 937 2074 2680 6889 620
Size: 317 1137 606 4209 2253
Fragments arranged by size:
4209 2253 1137 606 317
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 543 782 998 1211 1783 1849 2282 3404 3438
Size: 239 216 213 572 66 433 1122 34
Cuts at: 3438 3945 3963 4188 4890 5275 543
Size: 507 18 225 702 385 3790
Fragments arranged by size:
3790 1122 702 572 507 433 385 239
225 216 213 66 34 18
Bpu10I CC'TnA_GC
Cuts at: 243 1300 1844 3070 4161 4282 4694 243
Size: 1057 544 1226 1091 121 412 4071
Fragments arranged by size:
4071 1226 1091 1057 544 412 121
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 399 937 3329 5247 5698 6577 7069 7367 399
Size: 538 2392 1918 451 879 492 298 1554
Fragments arranged by size:
2392 1918 1554 879 538 492 451 298
BsaI GGTCTCn'nnnn_
Cuts at: 1227 3519 4670 5068 8226 1227
Size: 2292 1151 398 3158 1523
Fragments arranged by size:
3158 2292 1523 1151 398
BsaAI yAC'GTr
Cuts at: 1560 1836 4961 5875 1560
Size: 276 3125 914 4207
Fragments arranged by size:
4207 3125 914 276
BsaBI GATnn'nnATC
Cuts at: 6 1634 6036 8073 6
Size: 1628 4402 2037 455
Fragments arranged by size:
4402 2037 1628 455
BsaHI Gr'CG_yC
Cuts at: 1769 3465 4912 4943 5953 6201 6903 8399 1769
Size: 1696 1447 31 1010 248 702 1496 1892
Fragments arranged by size:
1892 1696 1496 1447 1010 702 248 31
BsaWI w'CCGG_w
Cuts at: 224 4262 5711 6232 6910 7184 7331 7742 224
Size: 4038 1449 521 678 274 147 411 1004
Fragments arranged by size:
4038 1449 1004 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 541 571 574 604 1475 1505 2036 2066 2189
Size: 30 3 30 871 30 531 30 123
Cuts at: 2189 2219 2861 2891 4780 4810 541
Size: 30 642 30 1889 30 4253
Fragments arranged by size:
4253 1889 871 642 531 123 30 30
30 30 30 30 30 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 975 1188 2882 2920 3225 3407 4479 4482 4491
Size: 213 1694 38 305 182 1072 3 9
Cuts at: 4491 4494 4497 4788 4793 5007 5292 5461 975
Size: 3 3 291 5 214 285 169 4036
Fragments arranged by size:
4036 1694 1072 305 291 285 214 213
182 169 38 9 5 3 3 3
BseYI C'CCAG_C
Cuts at: 586 2013 3651 3834 4111 4134 4819 5947 6705
Size: 1427 1638 183 277 23 685 1128 758
Cuts at: 6705 7282 7778 586
Size: 577 496 1330
Fragments arranged by size:
1638 1427 1330 1128 758 685 577 496
277 183 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 697 3183 3382 5431 697
Size: 2486 199 2049 3788
Fragments arranged by size:
3788 2486 2049 199
BsiEI CG_ry'CG
Cuts at: 104 6064 6110 7318 104
Size: 5960 46 1208 1308
Fragments arranged by size:
5960 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1739 1835 1850 1978 2085 2268 2333 2344 2666
Size: 96 15 128 107 183 65 11 322
Cuts at: 2666 2768 4390 4895 5536 6314 6504 7296 1739
Size: 102 1622 505 641 778 190 792 2965
Fragments arranged by size:
2965 1622 792 778 641 505 322 190
183 128 107 102 96 65 15 11
BsmI GAATG_Cn'
Cuts at: 1658 3393 3933 1658
Size: 1735 540 6247
Fragments arranged by size:
6247 1735 540
Bsp1286I G_dGCh'C
Cuts at: 1505 1739 1835 1850 1978 2085 2268 2333 2344
Size: 234 96 15 128 107 183 65 11
Cuts at: 2344 2666 2768 3044 3601 3874 3999 4390 4428
Size: 322 102 276 557 273 125 391 38
Cuts at: 4428 4895 5536 6147 6240 6314 6504 6566 7296
Size: 467 641 611 93 74 190 62 730
Cuts at: 7296 1505
Size: 2731
Fragments arranged by size:
2731 730 641 611 557 467 391 322
276 273 234 190 183 128 125 107
102 96 93 74 65 62 38 15
11
BspEI T'CCGG_A
Cuts at: 7742 7742
Size: 8522
BspHI T'CATG_A
Cuts at: 1739 1904 7698 1739
Size: 165 5794 2563
Fragments arranged by size:
5794 2563 165
BspMI ACCTGCnnnn'nnnn_
Cuts at: 5426 5572 5786 6088 6469 5426
Size: 146 214 302 381 7479
Fragments arranged by size:
7479 381 302 214 146
BsrBI CCG'CTC
Cuts at: 50 6814 6868 8352 8517 50
Size: 6764 54 1484 165 55
Fragments arranged by size:
6764 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1014 1335 1467 3737 3990 4644 6434 1014
Size: 321 132 2270 253 654 1790 3102
Fragments arranged by size:
3102 2270 1790 654 321 253 132
BsrFI r'CCGG_y
Cuts at: 4312 6520 6701 6910 4312
Size: 2208 181 209 5924
Fragments arranged by size:
5924 2208 209 181
BsrGI T'GTAC_A
Cuts at: 1991 2126 1991
Size: 135 8387
BssSI C'ACGA_G
Cuts at: 4611 6793 7151 4611
Size: 2182 358 5982
Fragments arranged by size:
5982 2182 358
BstAPI GCAn_nnn'nTGC
Cuts at: 346 2485 5091 5418 5899 346
Size: 2139 2606 327 481 2969
Fragments arranged by size:
2969 2606 2139 481 327
BstBI TT'CG_AA
Cuts at: 1662 6883 1662
Size: 5221 3301
BstXI CCAn_nnnn'nTGG
Cuts at: 2260 4410 2260
Size: 2150 6372
BstYI r'GATC_y
Cuts at: 1 1041 1563 1696 2550 2744 3095 3535 3646
Size: 1040 522 133 854 194 351 440 111
Cuts at: 3646 3897 4725 5547 6014 6037 6372 6618 7630
Size: 251 828 822 467 23 335 246 1012
Cuts at: 7630 7716 7728 8074 1
Size: 86 12 346 449
Fragments arranged by size:
1040 1012 854 828 822 522 467 449
440 351 346 335 251 246 194 133
111 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 922 2209 3773 5010 5434 922
Size: 1287 1564 1237 424 4010
Fragments arranged by size:
4010 1564 1287 1237 424
BtgI C'CryG_G
Cuts at: 1208 2516 2720 3205 3521 4116 4220 4251 4595
Size: 1308 204 485 316 595 104 31 344
Cuts at: 4595 1208
Size: 5135
Fragments arranged by size:
5135 1308 595 485 344 316 204 104
31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4646 5203 6472 6653 6816 4646
Size: 557 1269 181 163 6352
Fragments arranged by size:
6352 1269 557 181 163
BtsI GCAGTG_nn'
Cuts at: 709 5123 5765 709
Size: 4414 642 3466
Fragments arranged by size:
4414 3466 642
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2771 2806 2771
Size: 35 8487
DraI TTT'AAA
Cuts at: 5521 5521
Size: 8522
DrdI GACnn_nn'nnGTC
Cuts at: 540 6228 7086 540
Size: 5688 858 1976
Fragments arranged by size:
5688 1976 858
EaeI y'GGCC_r
Cuts at: 6107 6281 6672 6699 8044 6107
Size: 174 391 27 1345 6585
Fragments arranged by size:
6585 1345 391 174 27
EagI C'GGCC_G
Cuts at: 6107 6107
Size: 8522
EarI CTCTTCn'nnn_
Cuts at: 611 1979 4439 4500 4768 4954 6545 6755 611
Size: 1368 2460 61 268 186 1591 210 2378
Fragments arranged by size:
2460 2378 1591 1368 268 210 186 61
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3046 5419 6730 7040 7186 8332 3046
Size: 2373 1311 310 146 1146 3236
Fragments arranged by size:
3236 2373 1311 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1737 1848 2664 4388 5534 1737
Size: 111 816 1724 1146 4725
Fragments arranged by size:
4725 1724 1146 816 111
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 543 596 782 998 1211 1783 1849 2000 2282
Size: 53 186 216 213 572 66 151 282
Cuts at: 2282 2723 3404 3438 3945 3963 4188 4332 4890
Size: 441 681 34 507 18 225 144 558
Cuts at: 4890 4935 5275 5368 5383 5494 6347 6779 7526
Size: 45 340 93 15 111 853 432 747
Cuts at: 7526 7794 543
Size: 268 1271
Fragments arranged by size:
1271 853 747 681 572 558 507 441
432 340 282 268 225 216 213 186
151 144 111 93 66 53 45 34
18 15
EcoNI CCTnn'n_nnAGG
Cuts at: 4160 5422 4160
Size: 1262 7260
EcoO109I rG'GnC_Cy
Cuts at: 158 271 1076 1278 3331 3769 5661 158
Size: 113 805 202 2053 438 1892 3019
Fragments arranged by size:
3019 2053 1892 805 438 202 113
EcoRI G'AATT_C
Cuts at: 1284 2026 3277 5526 1284
Size: 742 1251 2249 4280
Fragments arranged by size:
4280 2249 1251 742
EcoRV GAT'ATC
Cuts at: 639 2235 639
Size: 1596 6926
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1530 2047 3306 4465 5413 6541 1530
Size: 517 1259 1159 948 1128 3511
Fragments arranged by size:
3511 1259 1159 1128 948 517
FspI TGC'GCA
Cuts at: 1006 6303 1006
Size: 5297 3225
HaeII r_GCGC'y
Cuts at: 222 3468 4544 5956 6204 7226 8205 222
Size: 3246 1076 1412 248 1022 979 539
Fragments arranged by size:
3246 1412 1076 1022 979 539 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 145 177 418 450 790 822 1042 1074 1742
Size: 32 241 32 340 32 220 32 668
Cuts at: 1742 1774 3402 3434 4084 4116 4605 4637 6364
Size: 32 1628 32 650 32 489 32 1727
Cuts at: 6364 6394 6396 6426 145
Size: 30 2 30 2241
Fragments arranged by size:
2241 1727 1628 668 650 489 340 241
220 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1781 3012 5561 1781
Size: 1231 2549 4742
Fragments arranged by size:
4742 2549 1231
HindIII A'AGCT_T
Cuts at: 768 768
Size: 8522
KasI G'GCGC_C
Cuts at: 3464 5952 6200 3464
Size: 2488 248 5786
Fragments arranged by size:
5786 2488 248
KpnI G_GTAC'C
Cuts at: 5542 5542
Size: 8522
MfeI C'AATT_G
Cuts at: 740 2243 2799 740
Size: 1503 556 6463
Fragments arranged by size:
6463 1503 556
MluI A'CGCG_T
Cuts at: 7955 7955
Size: 8522
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 524 844 850 3526 4841 5171 5264 7193 7377
Size: 320 6 2676 1315 330 93 1929 184
Cuts at: 7377 524
Size: 1669
Fragments arranged by size:
2676 1929 1669 1315 330 320 184 93
6
MscI TGG'CCA
Cuts at: 6283 6283
Size: 8522
MslI CAynn'nnrTG
Cuts at: 200 1109 3238 3505 4004 4191 5509 6511 6638
Size: 909 2129 267 499 187 1318 1002 127
Cuts at: 6638 200
Size: 2084
Fragments arranged by size:
2129 2084 1318 1002 909 499 267 187
127
MspA1I CmG'CkG
Cuts at: 1204 2008 3207 3698 4459 5253 5334 5633 5947
Size: 804 1199 491 761 794 81 299 314
Cuts at: 5947 6307 7320 7565 1204
Size: 360 1013 245 2161
Fragments arranged by size:
2161 1199 1013 804 794 761 491 360
314 299 245 81
NaeI GCC'GGC
Cuts at: 4314 6703 4314
Size: 2389 6133
NarI GG'CG_CC
Cuts at: 3465 5953 6201 3465
Size: 2488 248 5786
Fragments arranged by size:
5786 2488 248
NcoI C'CATG_G
Cuts at: 2516 2720 3521 4220 2516
Size: 204 801 699 6818
Fragments arranged by size:
6818 801 699 204
NgoMIV G'CCGG_C
Cuts at: 4312 6701 4312
Size: 2389 6133
NspI r_CATG'y
Cuts at: 216 1088 3237 3827 5575 6075 6982 216
Size: 872 2149 590 1748 500 907 1756
Fragments arranged by size:
2149 1756 1748 907 872 590 500
PciI A'CATG_T
Cuts at: 1084 6978 1084
Size: 5894 2628
PflMI CCAn_nnn'nTGG
Cuts at: 2687 4257 2687
Size: 1570 6952
PfoI T'CCnGG_A
Cuts at: 1760 2652 5069 1760
Size: 892 2417 5213
Fragments arranged by size:
5213 2417 892
PmeI GTTT'AAAC
Cuts at: 5521 5521
Size: 8522
PmlI CAC'GTG
Cuts at: 1836 1836
Size: 8522
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 541 573 2036 2068 2106 2138 7661 7693 541
Size: 32 1463 32 38 32 5523 32 1370
Fragments arranged by size:
5523 1463 1370 38 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 271 1278 3769 271
Size: 1007 2491 5024
Fragments arranged by size:
5024 2491 1007
PshAI GACnn'nnGTC
Cuts at: 1233 1233
Size: 8522
PspXI vC'TCGA_Gb
Cuts at: 3796 3951 3796
Size: 155 8367
PstI C_TGCA'G
Cuts at: 5133 5569 6254 5133
Size: 436 685 7401
Fragments arranged by size:
7401 685 436
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8522
PvuII CAG'CTG
Cuts at: 1204 2008 4459 5253 5334 5947 6307 1204
Size: 804 2451 794 81 613 360 3419
Fragments arranged by size:
3419 2451 804 794 613 360 81
RsrII CG'GwC_CG
Cuts at: 6717 6717
Size: 8522
SacI G_AGCT'C
Cuts at: 1739 1850 2666 4390 5536 1739
Size: 111 816 1724 1146 4725
Fragments arranged by size:
4725 1724 1146 816 111
SacII CC_GC'GG
Cuts at: 3208 3208
Size: 8522
SalI G'TCGA_C
Cuts at: 1779 5559 1779
Size: 3780 4742
SapI GCTCTTCn'nnn_
Cuts at: 611 4439 6545 6755 611
Size: 3828 2106 210 2378
Fragments arranged by size:
3828 2378 2106 210
SbfI CC_TGCA'GG
Cuts at: 5569 5569
Size: 8522
ScaI AGT'ACT
Cuts at: 1493 8039 1493
Size: 6546 1976
SexAI A'CCwGG_T
Cuts at: 2374 5150 2374
Size: 2776 5746
SfcI C'TryA_G
Cuts at: 33 266 2808 5129 5440 5565 6250 7243 7434
Size: 233 2542 2321 311 125 685 993 191
Cuts at: 7434 33
Size: 1121
Fragments arranged by size:
2542 2321 1121 993 685 311 233 191
125
SfoI GGC'GCC
Cuts at: 3466 5954 6202 3466
Size: 2488 248 5786
Fragments arranged by size:
5786 2488 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8522
SmaI CCC'GGG
Cuts at: 5544 5544
Size: 8522
SmlI C'TyrA_G
Cuts at: 414 952 3344 3796 3951 5226 5677 6592 7084
Size: 538 2392 452 155 1275 451 915 492
Cuts at: 7084 7346 414
Size: 262 1590
Fragments arranged by size:
2392 1590 1275 915 538 492 452 451
262 155
SpeI A'CTAG_T
Cuts at: 67 1729 67
Size: 1662 6860
SphI G_CATG'C
Cuts at: 3237 5575 6075 3237
Size: 2338 500 5684
Fragments arranged by size:
5684 2338 500
StuI AGG'CCT
Cuts at: 141 1808 4998 141
Size: 1667 3190 3665
Fragments arranged by size:
3665 3190 1667
StyI C'CwwG_G
Cuts at: 1217 1505 1886 2516 2720 3521 4220 4516 5656
Size: 288 381 630 204 801 699 296 1140
Cuts at: 5656 6010 1217
Size: 354 3729
Fragments arranged by size:
3729 1140 801 699 630 381 354 296
288 204
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1558 1746 1899 2202 3691 6105 6238 6697 6906
Size: 188 153 303 1489 2414 133 459 209
Cuts at: 6906 1558
Size: 3174
Fragments arranged by size:
3174 2414 1489 459 303 209 188 153
133
TatI w'GTAC_w
Cuts at: 1491 1955 1991 2126 3248 8037 1491
Size: 464 36 135 1122 4789 1976
Fragments arranged by size:
4789 1976 1122 464 135 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3332 3364 4780 4812 8039 8071 3332
Size: 32 1416 32 3227 32 3783
Fragments arranged by size:
3783 3227 1416 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1069 2350 6319 1069
Size: 1281 3969 3272
Fragments arranged by size:
3969 3272 1281
XbaI T'CTAG_A
Cuts at: 5553 5553
Size: 8522
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1512 3685 4593 6017 1512
Size: 2173 908 1424 4017
Fragments arranged by size:
4017 2173 1424 908
XhoI C'TCGA_G
Cuts at: 3796 3951 3796
Size: 155 8367
XmaI C'CCGG_G
Cuts at: 5542 5542
Size: 8522
XmnI GAAnn'nnTTC
Cuts at: 2030 2118 4448 5479 2030
Size: 88 2330 1031 5073
Fragments arranged by size:
5073 2330 1031 88
ZraI GAC'GTC
Cuts at: 4944 4944
Size: 8522
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspXI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI ApaI AscI BfrBI
BmgBI BmtI BsiWI BsmBI BssHII BstEII BstZ17I ClaI
DraIII FseI FspAI HpaI NdeI NheI NotI NruI
NsiI PacI PasI PsiI PspOMI PsrI SanDI SfiI
SgrAI SnaBI SrfI SspI SwaI