Restriction map for
pF1KA0447
(Circular) (Six-base) MAPSORT of: pF1KA0447.seq Check: 9154 from: 1 to: 4328
pF1KA0447 4328 bp
With 182 enzymes: SgfI *
September 18, 2009 16:59 ..
AatII G_ACGT'C
Cuts at: 947 947
Size: 4328
AccI GT'mk_AC
Cuts at: 1366 1366
Size: 4328
Acc65I G'GTAC_C
Cuts at: 1344 1344
Size: 4328
AclI AA'CG_TT
Cuts at: 4168 4168
Size: 4328
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 345 960 1018 2153 2585 3332 3600 345
Size: 615 58 1135 432 747 268 1073
Fragments arranged by size:
1135 1073 747 615 432 268 58
AflIII A'CryG_T
Cuts at: 1680 2784 3761 1680
Size: 1104 977 2247
Fragments arranged by size:
2247 1104 977
AgeI A'CCGG_T
Cuts at: 2716 2716
Size: 4328
AleI CACnn'nnGTG
Cuts at: 215 215
Size: 4328
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 868 900 868
Size: 32 4296
AlwNI CAG_nnn'CTG
Cuts at: 368 444 1248 1261 1633 3200 368
Size: 76 804 13 372 1567 1496
Fragments arranged by size:
1567 1496 804 372 76 13
ApaI G_GGCC'C
Cuts at: 1272 1272
Size: 4328
ApaLI G'TGCA_C
Cuts at: 744 3098 744
Size: 2354 1974
ApoI r'AATT_y
Cuts at: 1332 3681 3937 1332
Size: 2349 256 1723
Fragments arranged by size:
2349 1723 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4328
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4328
AvaI C'yCGr_G
Cuts at: 926 1348 926
Size: 422 3906
BamHI G'GATC_C
Cuts at: 1353 1353
Size: 4328
BanI G'GyrC_C
Cuts at: 1344 1758 2006 2041 1344
Size: 414 248 35 3631
Fragments arranged by size:
3631 414 248 35
BanII G_rGCy'C
Cuts at: 199 325 419 640 1272 1342 2372 199
Size: 126 94 221 632 70 1030 2155
Fragments arranged by size:
2155 1030 632 221 126 94 70
BbeI G_GCGC'C
Cuts at: 1762 2010 1762
Size: 248 4080
BciVI GTATCCnnnnn_n'
Cuts at: 2226 2987 4328 2226
Size: 761 1341 2226
Fragments arranged by size:
2226 1341 761
BclI T'GATC_A
Cuts at: 659 1848 659
Size: 1189 3139
BglII A'GATC_T
Cuts at: 1843 3880 1843
Size: 2037 2291
BlpI GC'TnA_GC
Cuts at: 844 1126 1440 844
Size: 282 314 3732
Fragments arranged by size:
3732 314 282
Bme1580I G_kGCm'C
Cuts at: 748 1272 1953 2046 3102 748
Size: 524 681 93 1056 1974
Fragments arranged by size:
1974 1056 681 524 93
BmgBI CAC'GTC
Cuts at: 993 993
Size: 4328
BmrI ACTGGGnnnn_n'
Cuts at: 715 943 1540 1621 1956 4100 715
Size: 228 597 81 335 2144 943
Fragments arranged by size:
2144 943 597 335 228 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2695 2695
Size: 4328
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1504 2383 2875 3173 1504
Size: 879 492 298 2659
Fragments arranged by size:
2659 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 280 4032 280
Size: 3752 576
BsaAI yAC'GTr
Cuts at: 1681 1681
Size: 4328
BsaBI GATnn'nnATC
Cuts at: 6 1842 3879 6
Size: 1836 2037 455
Fragments arranged by size:
2037 1836 455
BsaHI Gr'CG_yC
Cuts at: 944 1759 2007 2709 4205 944
Size: 815 248 702 1496 1067
Fragments arranged by size:
1496 1067 815 702 248
BsaWI w'CCGG_w
Cuts at: 1517 2038 2716 2990 3137 3548 1517
Size: 521 678 274 147 411 2297
Fragments arranged by size:
2297 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 868 898 868
Size: 30 4298
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 975 975
Size: 4328
BseYI C'CCAG_C
Cuts at: 129 191 405 418 1753 2511 3088 3584 129
Size: 62 214 13 1335 758 577 496 873
Fragments arranged by size:
1335 873 758 577 496 214 62 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 354 457 354
Size: 103 4225
BsiEI CG_ry'CG
Cuts at: 104 788 1169 1870 1916 3124 104
Size: 684 381 701 46 1208 1308
Fragments arranged by size:
1308 1208 701 684 381 46
BsiHKAI G_wGCw'C
Cuts at: 325 640 748 1342 2120 2310 3102 325
Size: 315 108 594 778 190 792 1551
Fragments arranged by size:
1551 792 778 594 315 190 108
BsiWI C'GTAC_G
Cuts at: 1049 1049
Size: 4328
BsmBI CGTCTCn'nnnn_
Cuts at: 619 619
Size: 4328
Bsp1286I G_dGCh'C
Cuts at: 199 325 419 640 748 1272 1342 1953 2046
Size: 126 94 221 108 524 70 611 93
Cuts at: 2046 2120 2310 2372 3102 199
Size: 74 190 62 730 1425
Fragments arranged by size:
1425 730 611 524 221 190 126 108
94 93 74 70 62
BspEI T'CCGG_A
Cuts at: 3548 3548
Size: 4328
BspHI T'CATG_A
Cuts at: 3504 3504
Size: 4328
BspMI ACCTGCnnnn'nnnn_
Cuts at: 167 1378 1592 1894 2275 167
Size: 1211 214 302 381 2220
Fragments arranged by size:
2220 1211 381 302 214
BsrBI CCG'CTC
Cuts at: 50 965 2620 2674 4158 4323 50
Size: 915 1655 54 1484 165 55
Fragments arranged by size:
1655 1484 915 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2240 2240
Size: 4328
BsrFI r'CCGG_y
Cuts at: 2326 2507 2716 2326
Size: 181 209 3938
Fragments arranged by size:
3938 209 181
BssSI C'ACGA_G
Cuts at: 2599 2957 2599
Size: 358 3970
BstAPI GCAn_nnn'nTGC
Cuts at: 1705 1705
Size: 4328
BstBI TT'CG_AA
Cuts at: 2689 2689
Size: 4328
BstEII G'GTnAC_C
Cuts at: 208 1185 208
Size: 977 3351
BstXI CCAn_nnnn'nTGG
Cuts at: 807 807
Size: 4328
BstYI r'GATC_y
Cuts at: 1 1353 1820 1843 2178 2424 3436 3522 3534
Size: 1352 467 23 335 246 1012 86 12
Cuts at: 3534 3880 1
Size: 346 449
Fragments arranged by size:
1352 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 290 452 290
Size: 162 4166
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2278 2459 2622 2278
Size: 181 163 3984
Fragments arranged by size:
3984 181 163
BtsI GCAGTG_nn'
Cuts at: 1571 1571
Size: 4328
DraI TTT'AAA
Cuts at: 1327 1327
Size: 4328
DraIII CAC_nnn'GTG
Cuts at: 350 350
Size: 4328
DrdI GACnn_nn'nnGTC
Cuts at: 2034 2892 2034
Size: 858 3470
EaeI y'GGCC_r
Cuts at: 749 785 908 1166 1263 1913 2087 2478 2505
Size: 36 123 258 97 650 174 391 27
Cuts at: 2505 3850 749
Size: 1345 1227
Fragments arranged by size:
1345 1227 650 391 258 174 123 97
36 27
EagI C'GGCC_G
Cuts at: 785 1166 1913 785
Size: 381 747 3200
Fragments arranged by size:
3200 747 381
EarI CTCTTCn'nnn_
Cuts at: 134 155 382 628 964 2351 2561 134
Size: 21 227 246 336 1387 210 1901
Fragments arranged by size:
1901 1387 336 246 227 210 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 630 2536 2846 2992 4138 630
Size: 1906 310 146 1146 820
Fragments arranged by size:
1906 1146 820 310 146
EcoICRI GAG'CTC
Cuts at: 323 638 1340 323
Size: 315 702 3311
Fragments arranged by size:
3311 702 315
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 345 960 1018 2153 2585 3332 3600 345
Size: 615 58 1135 432 747 268 1073
Fragments arranged by size:
1135 1073 747 615 432 268 58
EcoO109I rG'GnC_Cy
Cuts at: 1197 1269 1305 1467 1197
Size: 72 36 162 4058
Fragments arranged by size:
4058 162 72 36
EcoRI G'AATT_C
Cuts at: 1332 1332
Size: 4328
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2347 2347
Size: 4328
FspI TGC'GCA
Cuts at: 2109 2109
Size: 4328
HaeII r_GCGC'y
Cuts at: 334 742 899 1240 1762 2010 3032 4011 334
Size: 408 157 341 522 248 1022 979 651
Fragments arranged by size:
1022 979 651 522 408 341 248 157
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 651 683 2170 2200 2202 2232 651
Size: 32 1487 30 2 30 2747
Fragments arranged by size:
2747 1487 32 30 30 2
HincII GTy'rAC
Cuts at: 705 1367 705
Size: 662 3666
KasI G'GCGC_C
Cuts at: 1758 2006 1758
Size: 248 4080
KpnI G_GTAC'C
Cuts at: 1348 1348
Size: 4328
MluI A'CGCG_T
Cuts at: 3761 3761
Size: 4328
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2999 3183 2999
Size: 184 4144
MscI TGG'CCA
Cuts at: 910 2089 910
Size: 1179 3149
MslI CAynn'nnrTG
Cuts at: 215 2317 2444 215
Size: 2102 127 2099
Fragments arranged by size:
2102 2099 127
MspA1I CmG'CkG
Cuts at: 191 848 901 1293 1439 1753 2113 3126 3371
Size: 657 53 392 146 314 360 1013 245
Cuts at: 3371 191
Size: 1148
Fragments arranged by size:
1148 1013 657 392 360 314 245 146
53
NaeI GCC'GGC
Cuts at: 2509 2509
Size: 4328
NarI GG'CG_CC
Cuts at: 1759 2007 1759
Size: 248 4080
NgoMIV G'CCGG_C
Cuts at: 2507 2507
Size: 4328
NspI r_CATG'y
Cuts at: 426 1111 1381 1881 2788 426
Size: 685 270 500 907 1966
Fragments arranged by size:
1966 907 685 500 270
PciI A'CATG_T
Cuts at: 2784 2784
Size: 4328
PflMI CCAn_nnn'nTGG
Cuts at: 993 993
Size: 4328
PmeI GTTT'AAAC
Cuts at: 1327 1327
Size: 4328
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 868 900 3467 3499 868
Size: 32 2567 32 1697
Fragments arranged by size:
2567 1697 32 32
PpuMI rG'GwC_Cy
Cuts at: 1197 1305 1197
Size: 108 4220
PshAI GACnn'nnGTC
Cuts at: 292 292
Size: 4328
PspOMI G'GGCC_C
Cuts at: 1268 1268
Size: 4328
PstI C_TGCA'G
Cuts at: 884 1244 1257 1375 2060 884
Size: 360 13 118 685 3152
Fragments arranged by size:
3152 685 360 118 13
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4328
PvuII CAG'CTG
Cuts at: 191 1753 2113 191
Size: 1562 360 2406
Fragments arranged by size:
2406 1562 360
RsrII CG'GwC_CG
Cuts at: 2523 2523
Size: 4328
SacI G_AGCT'C
Cuts at: 325 640 1342 325
Size: 315 702 3311
Fragments arranged by size:
3311 702 315
SalI G'TCGA_C
Cuts at: 1365 1365
Size: 4328
SapI GCTCTTCn'nnn_
Cuts at: 134 382 628 964 2351 2561 134
Size: 248 246 336 1387 210 1901
Fragments arranged by size:
1901 1387 336 248 246 210
SbfI CC_TGCA'GG
Cuts at: 1375 1375
Size: 4328
ScaI AGT'ACT
Cuts at: 3845 3845
Size: 4328
SfcI C'TryA_G
Cuts at: 33 880 1240 1253 1371 2056 3049 3240 33
Size: 847 360 13 118 685 993 191 1121
Fragments arranged by size:
1121 993 847 685 360 191 118 13
SfoI GGC'GCC
Cuts at: 1760 2008 1760
Size: 248 4080
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4328
SmaI CCC'GGG
Cuts at: 928 1350 928
Size: 422 3906
SmlI C'TyrA_G
Cuts at: 1483 2398 2890 3152 1483
Size: 915 492 262 2659
Fragments arranged by size:
2659 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4328
SphI G_CATG'C
Cuts at: 426 1381 1881 426
Size: 955 500 2873
Fragments arranged by size:
2873 955 500
StyI C'CwwG_G
Cuts at: 1462 1816 1462
Size: 354 3974
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 301 540 1911 2044 2503 2712 301
Size: 239 1371 133 459 209 1917
Fragments arranged by size:
1917 1371 459 239 209 133
TatI w'GTAC_w
Cuts at: 389 686 3843 389
Size: 297 3157 874
Fragments arranged by size:
3157 874 297
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 515 547 3845 3877 515
Size: 32 3298 32 966
Fragments arranged by size:
3298 966 32 32
Tth111I GACn'n_nGTC
Cuts at: 1018 2125 1018
Size: 1107 3221
XbaI T'CTAG_A
Cuts at: 1359 1359
Size: 4328
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 804 1823 804
Size: 1019 3309
XmaI C'CCGG_G
Cuts at: 926 1348 926
Size: 422 3906
XmnI GAAnn'nnTTC
Cuts at: 973 973
Size: 4328
ZraI GAC'GTC
Cuts at: 945 945
Size: 4328
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PflMI PmeI
PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AlfI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BglI BmtI BpmI Bpu10I
BsmI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PfoI PmlI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI