Restriction map for
pF1KA0456
(Circular) (Six-base) MAPSORT of: pF1KA0456.seq Check: 6477 from: 1 to: 6326
pF1KA0456 6326 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AatII G_ACGT'C
Cuts at: 115 115
Size: 6326
AccI GT'mk_AC
Cuts at: 2174 3298 3364 2174
Size: 1124 66 5136
Fragments arranged by size:
5136 1124 66
Acc65I G'GTAC_C
Cuts at: 3342 3342
Size: 6326
AclI AA'CG_TT
Cuts at: 743 6166 743
Size: 5423 903
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 859 1822 2552 2857 4151 4583 4936 5598 859
Size: 963 730 305 1294 432 353 662 1587
Fragments arranged by size:
1587 1294 963 730 662 432 353 305
AflIII A'CryG_T
Cuts at: 611 762 3678 5478 5759 611
Size: 151 2916 1800 281 1178
Fragments arranged by size:
2916 1800 1178 281 151
AhdI GACnn_n'nnGTC
Cuts at: 2079 2079
Size: 6326
AlwNI CAG_nnn'CTG
Cuts at: 2635 3631 5069 2635
Size: 996 1438 3892
Fragments arranged by size:
3892 1438 996
ApaI G_GGCC'C
Cuts at: 2045 2170 2045
Size: 125 6201
ApaLI G'TGCA_C
Cuts at: 5164 5164
Size: 6326
ApoI r'AATT_y
Cuts at: 124 1679 3330 5679 5935 124
Size: 1555 1651 2349 256 515
Fragments arranged by size:
2349 1651 1555 515 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6326
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6326
AvaI C'yCGr_G
Cuts at: 2339 3118 3346 2339
Size: 779 228 5319
Fragments arranged by size:
5319 779 228
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 152 185 152
Size: 33 6293
BamHI G'GATC_C
Cuts at: 1696 3351 1696
Size: 1655 4671
BanI G'GyrC_C
Cuts at: 3342 3756 4004 4039 3342
Size: 414 248 35 5629
Fragments arranged by size:
5629 414 248 35
BanII G_rGCy'C
Cuts at: 587 778 981 1902 2045 2170 2297 3044 3083
Size: 191 203 921 143 125 127 747 39
Cuts at: 3083 3125 3340 4370 587
Size: 42 215 1030 2543
Fragments arranged by size:
2543 1030 921 747 215 203 191 143
127 125 42 39
BbeI G_GCGC'C
Cuts at: 3760 4008 3760
Size: 248 6078
BbsI GAAGACnn'nnnn_
Cuts at: 522 607 891 1107 1279 2246 2875 3234 522
Size: 85 284 216 172 967 629 359 3614
Fragments arranged by size:
3614 967 629 359 284 216 172 85
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2290 2324 2290
Size: 34 6292
BciVI GTATCCnnnnn_n'
Cuts at: 1651 4224 5280 6326 1651
Size: 2573 1056 1046 1651
Fragments arranged by size:
2573 1651 1056 1046
BclI T'GATC_A
Cuts at: 936 2108 3846 936
Size: 1172 1738 3416
Fragments arranged by size:
3416 1738 1172
BfrBI ATG'CAT
Cuts at: 892 892
Size: 6326
BglII A'GATC_T
Cuts at: 3841 5878 3841
Size: 2037 4289
BlpI GC'TnA_GC
Cuts at: 185 2677 3438 185
Size: 2492 761 3073
Fragments arranged by size:
3073 2492 761
Bme1580I G_kGCm'C
Cuts at: 1182 2045 2170 2801 3951 4044 5168 1182
Size: 863 125 631 1150 93 1124 2340
Fragments arranged by size:
2340 1150 1124 863 631 125 93
BmgBI CAC'GTC
Cuts at: 3021 3074 3021
Size: 53 6273
BmrI ACTGGGnnnn_n'
Cuts at: 2564 3052 3538 3619 3954 6098 2564
Size: 488 486 81 335 2144 2792
Fragments arranged by size:
2792 2144 488 486 335 81
BmtI G_CTAG'C
Cuts at: 2723 2723
Size: 6326
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 606 4693 606
Size: 4087 2239
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 101 238 328 374 1501 1590 2182 3069 101
Size: 137 90 46 1127 89 592 887 3358
Fragments arranged by size:
3358 1127 887 592 137 90 89 46
Bpu10I CC'TnA_GC
Cuts at: 2936 2936
Size: 6326
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3094 3502 4381 5095 5393 3094
Size: 408 879 714 298 4027
Fragments arranged by size:
4027 879 714 408 298
BsaI GGTCTCn'nnnn_
Cuts at: 1389 6030 1389
Size: 4641 1685
BsaAI yAC'GTr
Cuts at: 2097 2325 3679 2097
Size: 228 1354 4744
Fragments arranged by size:
4744 1354 228
BsaBI GATnn'nnATC
Cuts at: 6 2594 3840 5877 6
Size: 2588 1246 2037 455
Fragments arranged by size:
2588 2037 1246 455
BsaHI Gr'CG_yC
Cuts at: 112 3757 4005 4707 6203 112
Size: 3645 248 702 1496 235
Fragments arranged by size:
3645 1496 702 248 235
BsaWI w'CCGG_w
Cuts at: 767 1643 1910 2645 2864 3515 4036 4714 5125
Size: 876 267 735 219 651 521 678 411
Cuts at: 5125 5272 5546 767
Size: 147 274 1547
Fragments arranged by size:
1547 876 735 678 651 521 411 274
267 219 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1342 1372 2686 2716 1342
Size: 30 1314 30 4952
Fragments arranged by size:
4952 1314 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 400 685 769 786 1437 1754 2198 2688 2691
Size: 285 84 17 651 317 444 490 3
Cuts at: 2691 3097 400
Size: 406 3629
Fragments arranged by size:
3629 651 490 444 406 317 285 84
17 3
BseYI C'CCAG_C
Cuts at: 1169 1181 1408 1756 2738 3101 3151 3751 4509
Size: 12 227 348 982 363 50 600 758
Cuts at: 4509 5174 5582 1169
Size: 665 408 1913
Fragments arranged by size:
1913 982 758 665 600 408 363 348
227 50 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1888 3079 1888
Size: 1191 5135
BsiEI CG_ry'CG
Cuts at: 104 3868 3914 5144 104
Size: 3764 46 1230 1286
Fragments arranged by size:
3764 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 186 587 639 778 1902 2270 3340 4118 4308
Size: 401 52 139 1124 368 1070 778 190
Cuts at: 4308 5168 186
Size: 860 1344
Fragments arranged by size:
1344 1124 1070 860 778 401 368 190
139 52
BsmI GAATG_Cn'
Cuts at: 1056 1056
Size: 6326
BsmBI CGTCTCn'nnnn_
Cuts at: 119 119
Size: 6326
Bsp1286I G_dGCh'C
Cuts at: 186 587 639 778 981 1182 1902 2045 2170
Size: 401 52 139 203 201 720 143 125
Cuts at: 2170 2270 2297 2801 3044 3083 3125 3340 3951
Size: 100 27 504 243 39 42 215 611
Cuts at: 3951 4044 4118 4308 4370 5168 186
Size: 93 74 190 62 798 1344
Fragments arranged by size:
1344 798 720 611 504 401 243 215
203 201 190 143 139 125 100 93
74 62 52 42 39 27
BspEI T'CCGG_A
Cuts at: 767 1910 2645 2864 767
Size: 1143 735 219 4229
Fragments arranged by size:
4229 1143 735 219
BspHI T'CATG_A
Cuts at: 1397 1854 1952 2747 4758 1397
Size: 457 98 795 2011 2965
Fragments arranged by size:
2965 2011 795 457 98
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1895 3376 3590 3892 4273 1895
Size: 1481 214 302 381 3948
Fragments arranged by size:
3948 1481 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2554 4618 4672 6156 6321 50
Size: 2504 2064 54 1484 165 55
Fragments arranged by size:
2504 2064 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1412 2306 2930 4238 1412
Size: 894 624 1308 3500
Fragments arranged by size:
3500 1308 894 624
BsrFI r'CCGG_y
Cuts at: 707 2411 4324 4505 707
Size: 1704 1913 181 2528
Fragments arranged by size:
2528 1913 1704 181
BsrGI T'GTAC_A
Cuts at: 492 614 1103 492
Size: 122 489 5715
Fragments arranged by size:
5715 489 122
BssSI C'ACGA_G
Cuts at: 2274 4597 5305 2274
Size: 2323 708 3295
Fragments arranged by size:
3295 2323 708
BstAPI GCAn_nnn'nTGC
Cuts at: 243 889 3703 243
Size: 646 2814 2866
Fragments arranged by size:
2866 2814 646
BstBI TT'CG_AA
Cuts at: 4687 4687
Size: 6326
BstXI CCAn_nnnn'nTGG
Cuts at: 2160 2160
Size: 6326
BstYI r'GATC_y
Cuts at: 1 176 1696 2861 3351 3818 3841 4176 4422
Size: 175 1520 1165 490 467 23 335 246
Cuts at: 4422 4728 4740 4826 5878 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1520 1165 1052 490 467 449 335 306
246 175 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2924 2924
Size: 6326
BtgI C'CryG_G
Cuts at: 734 1361 1379 2670 2915 734
Size: 627 18 1291 245 4145
Fragments arranged by size:
4145 1291 627 245 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2294 3144 4276 4457 4620 2294
Size: 850 1132 181 163 4000
Fragments arranged by size:
4000 1132 850 181 163
BtsI GCAGTG_nn'
Cuts at: 226 3006 3569 226
Size: 2780 563 2983
Fragments arranged by size:
2983 2780 563
DraI TTT'AAA
Cuts at: 525 3325 525
Size: 2800 3526
DrdI GACnn_nn'nnGTC
Cuts at: 2926 4032 5376 2926
Size: 1106 1344 3876
Fragments arranged by size:
3876 1344 1106
EaeI y'GGCC_r
Cuts at: 332 543 737 3911 4085 4476 4503 5848 332
Size: 211 194 3174 174 391 27 1345 810
Fragments arranged by size:
3174 1345 810 391 211 194 174 27
EagI C'GGCC_G
Cuts at: 3911 3911
Size: 6326
EarI CTCTTCn'nnn_
Cuts at: 1256 1840 1994 4349 4559 1256
Size: 584 154 2355 210 3023
Fragments arranged by size:
3023 2355 584 210 154
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 673 4534 5276 5422 6136 673
Size: 3861 742 146 714 863
Fragments arranged by size:
3861 863 742 714 146
EcoICRI GAG'CTC
Cuts at: 585 776 1900 3338 585
Size: 191 1124 1438 3573
Fragments arranged by size:
3573 1438 1124 191
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 101 238 328 374 859 1501 1590 1822 2182
Size: 137 90 46 485 642 89 232 360
Cuts at: 2182 2552 2857 3069 4151 4583 4936 5598 101
Size: 370 305 212 1082 432 353 662 829
Fragments arranged by size:
1082 829 662 642 485 432 370 360
353 305 232 212 137 90 89 46
EcoO109I rG'GnC_Cy
Cuts at: 249 573 1746 2042 2166 2167 3114 3465 249
Size: 324 1173 296 124 1 947 351 3110
Fragments arranged by size:
3110 1173 947 351 324 296 124 1
EcoRI G'AATT_C
Cuts at: 3330 3330
Size: 6326
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1827 1866 4345 1827
Size: 39 2479 3808
Fragments arranged by size:
3808 2479 39
FspI TGC'GCA
Cuts at: 4107 4107
Size: 6326
HaeII r_GCGC'y
Cuts at: 1092 3143 3760 4008 5238 6009 1092
Size: 2051 617 248 1230 771 1409
Fragments arranged by size:
2051 1409 1230 771 617 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 168 200 2065 2097 2686 2718 4168 4198 4200
Size: 32 1865 32 589 32 1450 30 2
Cuts at: 4200 4230 168
Size: 30 2264
Fragments arranged by size:
2264 1865 1450 589 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 396 3276 3365 396
Size: 2880 89 3357
Fragments arranged by size:
3357 2880 89
HindIII A'AGCT_T
Cuts at: 1804 2895 1804
Size: 1091 5235
KasI G'GCGC_C
Cuts at: 3756 4004 3756
Size: 248 6078
KpnI G_GTAC'C
Cuts at: 3346 3346
Size: 6326
MluI A'CGCG_T
Cuts at: 5759 5759
Size: 6326
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2417 5085 5269 2417
Size: 2668 184 3474
Fragments arranged by size:
3474 2668 184
MscI TGG'CCA
Cuts at: 334 4087 334
Size: 3753 2573
MslI CAynn'nnrTG
Cuts at: 1099 1465 2099 4315 4442 1099
Size: 366 634 2216 127 2983
Fragments arranged by size:
2983 2216 634 366 127
MspA1I CmG'CkG
Cuts at: 1554 2385 2564 2881 3437 3751 4111 4895 5140
Size: 831 179 317 556 314 360 784 245
Cuts at: 5140 1554
Size: 2740
Fragments arranged by size:
2740 831 784 556 360 317 314 245
179
NaeI GCC'GGC
Cuts at: 2413 4507 2413
Size: 2094 4232
NarI GG'CG_CC
Cuts at: 3757 4005 3757
Size: 248 6078
NcoI C'CATG_G
Cuts at: 1361 2670 2915 1361
Size: 1309 245 4772
Fragments arranged by size:
4772 1309 245
NgoMIV G'CCGG_C
Cuts at: 2411 4505 2411
Size: 2094 4232
NheI G'CTAG_C
Cuts at: 2719 2719
Size: 6326
NsiI A_TGCA'T
Cuts at: 894 894
Size: 6326
NspI r_CATG'y
Cuts at: 615 766 3379 3879 5482 615
Size: 151 2613 500 1603 1459
Fragments arranged by size:
2613 1603 1459 500 151
PasI CC'CwG_GG
Cuts at: 1539 2153 2707 1539
Size: 614 554 5158
Fragments arranged by size:
5158 614 554
PciI A'CATG_T
Cuts at: 611 762 5478 611
Size: 151 4716 1459
Fragments arranged by size:
4716 1459 151
PflMI CCAn_nnn'nTGG
Cuts at: 1779 2635 1779
Size: 856 5470
PfoI T'CCnGG_A
Cuts at: 254 1301 254
Size: 1047 5279
PmeI GTTT'AAAC
Cuts at: 3325 3325
Size: 6326
PmlI CAC'GTG
Cuts at: 2097 2097
Size: 6326
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1817 1849 4772 4804 1817
Size: 32 2923 32 3339
Fragments arranged by size:
3339 2923 32 32
PpuMI rG'GwC_Cy
Cuts at: 249 573 1746 3114 249
Size: 324 1173 1368 3461
Fragments arranged by size:
3461 1368 1173 324
PspOMI G'GGCC_C
Cuts at: 2041 2166 2041
Size: 125 6201
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 571 603 571
Size: 32 6294
PstI C_TGCA'G
Cuts at: 1229 1532 1892 3373 4058 1229
Size: 303 360 1481 685 3497
Fragments arranged by size:
3497 1481 685 360 303
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6326
PvuII CAG'CTG
Cuts at: 2564 3751 4111 2564
Size: 1187 360 4779
Fragments arranged by size:
4779 1187 360
RsrII CG'GwC_CG
Cuts at: 4521 4521
Size: 6326
SacI G_AGCT'C
Cuts at: 587 778 1902 3340 587
Size: 191 1124 1438 3573
Fragments arranged by size:
3573 1438 1124 191
SalI G'TCGA_C
Cuts at: 3363 3363
Size: 6326
SapI GCTCTTCn'nnn_
Cuts at: 4349 4559 4349
Size: 210 6116
SbfI CC_TGCA'GG
Cuts at: 1892 3373 1892
Size: 1481 4845
ScaI AGT'ACT
Cuts at: 865 907 3147 5843 865
Size: 42 2240 2696 1348
Fragments arranged by size:
2696 2240 1348 42
SexAI A'CCwGG_T
Cuts at: 419 2075 419
Size: 1656 4670
SfcI C'TryA_G
Cuts at: 33 1225 1528 1666 1888 2175 3369 4054 5022
Size: 1192 303 138 222 287 1194 685 968
Cuts at: 5022 5213 33
Size: 191 1146
Fragments arranged by size:
1194 1192 1146 968 685 303 287 222
191 138
SfoI GGC'GCC
Cuts at: 3758 4006 3758
Size: 248 6078
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6326
SmaI CCC'GGG
Cuts at: 3348 3348
Size: 6326
SmlI C'TyrA_G
Cuts at: 3109 3481 4396 5110 5372 3109
Size: 372 915 714 262 4063
Fragments arranged by size:
4063 915 714 372 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6326
SphI G_CATG'C
Cuts at: 3379 3879 3379
Size: 500 5826
StyI C'CwwG_G
Cuts at: 350 1361 1841 2670 2915 3035 3460 3814 350
Size: 1011 480 829 245 120 425 354 2862
Fragments arranged by size:
2862 1011 829 480 425 354 245 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3115 3909 4042 4501 4710 3115
Size: 794 133 459 209 4731
Fragments arranged by size:
4731 794 459 209 133
TatI w'GTAC_w
Cuts at: 492 614 821 863 905 1103 1349 2447 3145
Size: 122 207 42 42 198 246 1098 698
Cuts at: 3145 3313 5841 492
Size: 168 2528 977
Fragments arranged by size:
2528 1098 977 698 246 207 198 168
122 42 42
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 205 237 1688 1720 5843 5875 205
Size: 32 1451 32 4123 32 656
Fragments arranged by size:
4123 1451 656 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2476 4123 2476
Size: 1647 4679
XbaI T'CTAG_A
Cuts at: 3357 3357
Size: 6326
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 398 3821 398
Size: 3423 2903
XmaI C'CCGG_G
Cuts at: 3346 3346
Size: 6326
XmnI GAAnn'nnTTC
Cuts at: 2140 2140
Size: 6326
ZraI GAC'GTC
Cuts at: 113 113
Size: 6326
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AleI AlfI AloI AscI
AvrII BbvCI BglI BsiWI BssHII BstEII BstZ17I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI
NdeI NotI NruI PacI PshAI PsiI PspXI SacII
SanDI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI