Restriction map for
pF1KA0459
(Circular) (Six-base) MAPSORT of: pF1KA0459.seq Check: 3808 from: 1 to: 4307
pF1KA0459 4307 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 702 702
Size: 4307
AccI GT'mk_AC
Cuts at: 1345 1345
Size: 4307
Acc65I G'GTAC_C
Cuts at: 1323 1323
Size: 4307
AclI AA'CG_TT
Cuts at: 4147 4147
Size: 4307
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 800 1000 2132 2564 3311 3579 322
Size: 478 200 1132 432 747 268 1050
Fragments arranged by size:
1132 1050 747 478 432 268 200
AflIII A'CryG_T
Cuts at: 1659 2763 3740 1659
Size: 1104 977 2226
Fragments arranged by size:
2226 1104 977
AgeI A'CCGG_T
Cuts at: 2695 2695
Size: 4307
AleI CACnn'nnGTG
Cuts at: 1273 1273
Size: 4307
AlwNI CAG_nnn'CTG
Cuts at: 294 870 1612 3179 294
Size: 576 742 1567 1422
Fragments arranged by size:
1567 1422 742 576
ApaI G_GGCC'C
Cuts at: 204 204
Size: 4307
ApaLI G'TGCA_C
Cuts at: 3077 3077
Size: 4307
ApoI r'AATT_y
Cuts at: 1311 3660 3916 1311
Size: 2349 256 1702
Fragments arranged by size:
2349 1702 256
AscI GG'CGCG_CC
Cuts at: 193 193
Size: 4307
AseI AT'TA_AT
Cuts at: 20 20
Size: 4307
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4307
AvaI C'yCGr_G
Cuts at: 1327 1327
Size: 4307
BamHI G'GATC_C
Cuts at: 1332 1332
Size: 4307
BanI G'GyrC_C
Cuts at: 315 690 1018 1190 1323 1737 1985 2020 315
Size: 375 328 172 133 414 248 35 2602
Fragments arranged by size:
2602 414 375 328 248 172 133 35
BanII G_rGCy'C
Cuts at: 204 348 965 1321 2351 204
Size: 144 617 356 1030 2160
Fragments arranged by size:
2160 1030 617 356 144
BbeI G_GCGC'C
Cuts at: 1741 1989 1741
Size: 248 4059
BbsI GAAGACnn'nnnn_
Cuts at: 788 1285 788
Size: 497 3810
BciVI GTATCCnnnnn_n'
Cuts at: 2205 2966 4307 2205
Size: 761 1341 2205
Fragments arranged by size:
2205 1341 761
BclI T'GATC_A
Cuts at: 354 1827 354
Size: 1473 2834
BglI GCCn_nnn'nGGC
Cuts at: 140 487 140
Size: 347 3960
BglII A'GATC_T
Cuts at: 1822 3859 1822
Size: 2037 2270
BlpI GC'TnA_GC
Cuts at: 1419 1419
Size: 4307
Bme1580I G_kGCm'C
Cuts at: 204 1932 2025 3081 204
Size: 1728 93 1056 1430
Fragments arranged by size:
1728 1430 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 959 1519 1600 1935 4079 959
Size: 560 81 335 2144 1187
Fragments arranged by size:
2144 1187 560 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 387 2674 387
Size: 2287 2020
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 1228 688
Size: 540 3767
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 979 1483 2362 2854 3152 370
Size: 609 504 879 492 298 1525
Fragments arranged by size:
1525 879 609 504 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4011 4011
Size: 4307
BsaAI yAC'GTr
Cuts at: 1660 1660
Size: 4307
BsaBI GATnn'nnATC
Cuts at: 6 1821 3858 6
Size: 1815 2037 455
Fragments arranged by size:
2037 1815 455
BsaHI Gr'CG_yC
Cuts at: 1738 1986 2688 4184 1738
Size: 248 702 1496 1861
Fragments arranged by size:
1861 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1496 2017 2695 2969 3116 3527 1496
Size: 521 678 274 147 411 2276
Fragments arranged by size:
2276 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 271 274 1018 1198 271
Size: 3 744 180 3380
Fragments arranged by size:
3380 744 180 3
BseYI C'CCAG_C
Cuts at: 1732 2490 3067 3563 1732
Size: 758 577 496 2476
Fragments arranged by size:
2476 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 680 680
Size: 4307
BsiEI CG_ry'CG
Cuts at: 104 139 229 1849 1895 3103 104
Size: 35 90 1620 46 1208 1308
Fragments arranged by size:
1620 1308 1208 90 46 35
BsiHKAI G_wGCw'C
Cuts at: 348 653 1321 2099 2289 3081 348
Size: 305 668 778 190 792 1574
Fragments arranged by size:
1574 792 778 668 305 190
Bsp1286I G_dGCh'C
Cuts at: 204 348 653 965 1321 1932 2025 2099 2289
Size: 144 305 312 356 611 93 74 190
Cuts at: 2289 2351 3081 204
Size: 62 730 1430
Fragments arranged by size:
1430 730 611 356 312 305 190 144
93 74 62
BspEI T'CCGG_A
Cuts at: 3527 3527
Size: 4307
BspHI T'CATG_A
Cuts at: 3483 3483
Size: 4307
BspMI ACCTGCnnnn'nnnn_
Cuts at: 702 1254 1357 1571 1873 2254 702
Size: 552 103 214 302 381 2755
Fragments arranged by size:
2755 552 381 302 214 103
BsrBI CCG'CTC
Cuts at: 50 186 216 1291 2599 2653 4137 4302 50
Size: 136 30 1075 1308 54 1484 165 55
Fragments arranged by size:
1484 1308 1075 165 136 55 54 30
BsrDI GCAATG_nn'
Cuts at: 519 2219 519
Size: 1700 2607
BsrFI r'CCGG_y
Cuts at: 134 2305 2486 2695 134
Size: 2171 181 209 1746
Fragments arranged by size:
2171 1746 209 181
BssHII G'CGCG_C
Cuts at: 193 193
Size: 4307
BssSI C'ACGA_G
Cuts at: 2578 2936 2578
Size: 358 3949
BstAPI GCAn_nnn'nTGC
Cuts at: 907 1684 907
Size: 777 3530
BstBI TT'CG_AA
Cuts at: 2668 2668
Size: 4307
BstEII G'GTnAC_C
Cuts at: 1266 1266
Size: 4307
BstYI r'GATC_y
Cuts at: 1 1332 1799 1822 2157 2403 3415 3501 3513
Size: 1331 467 23 335 246 1012 86 12
Cuts at: 3513 3859 1
Size: 346 449
Fragments arranged by size:
1331 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 615 2257 2438 2601 615
Size: 1642 181 163 2321
Fragments arranged by size:
2321 1642 181 163
BtsI GCAGTG_nn'
Cuts at: 991 1020 1162 1550 991
Size: 29 142 388 3748
Fragments arranged by size:
3748 388 142 29
DraI TTT'AAA
Cuts at: 1306 1306
Size: 4307
DrdI GACnn_nn'nnGTC
Cuts at: 2013 2871 2013
Size: 858 3449
EaeI y'GGCC_r
Cuts at: 136 206 226 479 1892 2066 2457 2484 3829
Size: 70 20 253 1413 174 391 27 1345
Cuts at: 3829 136
Size: 614
Fragments arranged by size:
1413 1345 614 391 253 174 70 27
20
EagI C'GGCC_G
Cuts at: 136 226 1892 136
Size: 90 1666 2551
Fragments arranged by size:
2551 1666 90
EarI CTCTTCn'nnn_
Cuts at: 124 928 944 2330 2540 124
Size: 804 16 1386 210 1891
Fragments arranged by size:
1891 1386 804 210 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 234 2515 2825 2971 4117 234
Size: 2281 310 146 1146 424
Fragments arranged by size:
2281 1146 424 310 146
EcoICRI GAG'CTC
Cuts at: 346 1319 346
Size: 973 3334
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 688 800 1000 1228 2132 2564 3311 3579
Size: 366 112 200 228 904 432 747 268
Cuts at: 3579 322
Size: 1050
Fragments arranged by size:
1050 904 747 432 366 268 228 200
112
EcoNI CCTnn'n_nnAGG
Cuts at: 208 208
Size: 4307
EcoO109I rG'GnC_Cy
Cuts at: 201 291 1446 201
Size: 90 1155 3062
Fragments arranged by size:
3062 1155 90
EcoRI G'AATT_C
Cuts at: 1311 1311
Size: 4307
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 973 2326 973
Size: 1353 2954
FspI TGC'GCA
Cuts at: 2088 2088
Size: 4307
HaeII r_GCGC'y
Cuts at: 1741 1989 3011 3990 1741
Size: 248 1022 979 2058
Fragments arranged by size:
2058 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 711 740 743 772 2149 2179 2181 2211 711
Size: 29 3 29 1377 30 2 30 2807
Fragments arranged by size:
2807 1377 30 30 29 29 3 2
HincII GTy'rAC
Cuts at: 1346 1346
Size: 4307
KasI G'GCGC_C
Cuts at: 1737 1985 1737
Size: 248 4059
KpnI G_GTAC'C
Cuts at: 1327 1327
Size: 4307
MfeI C'AATT_G
Cuts at: 330 358 521 330
Size: 28 163 4116
Fragments arranged by size:
4116 163 28
MluI A'CGCG_T
Cuts at: 3740 3740
Size: 4307
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2978 3162 2978
Size: 184 4123
MscI TGG'CCA
Cuts at: 208 481 2068 208
Size: 273 1587 2447
Fragments arranged by size:
2447 1587 273
MslI CAynn'nnrTG
Cuts at: 731 1273 2296 2423 731
Size: 542 1023 127 2615
Fragments arranged by size:
2615 1023 542 127
MspA1I CmG'CkG
Cuts at: 146 285 426 1418 1732 2092 3105 3350 146
Size: 139 141 992 314 360 1013 245 1103
Fragments arranged by size:
1103 1013 992 360 314 245 141 139
NaeI GCC'GGC
Cuts at: 136 2488 136
Size: 2352 1955
NarI GG'CG_CC
Cuts at: 1738 1986 1738
Size: 248 4059
NgoMIV G'CCGG_C
Cuts at: 134 2486 134
Size: 2352 1955
NspI r_CATG'y
Cuts at: 478 1360 1860 2767 478
Size: 882 500 907 2018
Fragments arranged by size:
2018 907 882 500
PasI CC'CwG_GG
Cuts at: 294 294
Size: 4307
PciI A'CATG_T
Cuts at: 2763 2763
Size: 4307
PflMI CCAn_nnn'nTGG
Cuts at: 870 870
Size: 4307
PmeI GTTT'AAAC
Cuts at: 1306 1306
Size: 4307
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 247 279 3446 3478 247
Size: 32 3167 32 1076
Fragments arranged by size:
3167 1076 32 32
PsiI TTA'TAA
Cuts at: 887 887
Size: 4307
PspOMI G'GGCC_C
Cuts at: 200 200
Size: 4307
PstI C_TGCA'G
Cuts at: 290 1354 2039 290
Size: 1064 685 2558
Fragments arranged by size:
2558 1064 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4307
PvuII CAG'CTG
Cuts at: 285 1732 2092 285
Size: 1447 360 2500
Fragments arranged by size:
2500 1447 360
RsrII CG'GwC_CG
Cuts at: 2502 2502
Size: 4307
SacI G_AGCT'C
Cuts at: 348 1321 348
Size: 973 3334
SalI G'TCGA_C
Cuts at: 1344 1344
Size: 4307
SapI GCTCTTCn'nnn_
Cuts at: 124 2330 2540 124
Size: 2206 210 1891
Fragments arranged by size:
2206 1891 210
SbfI CC_TGCA'GG
Cuts at: 1354 1354
Size: 4307
ScaI AGT'ACT
Cuts at: 3824 3824
Size: 4307
SfcI C'TryA_G
Cuts at: 33 286 1350 2035 3028 3219 33
Size: 253 1064 685 993 191 1121
Fragments arranged by size:
1121 1064 993 685 253 191
SfoI GGC'GCC
Cuts at: 1739 1987 1739
Size: 248 4059
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4307
SmaI CCC'GGG
Cuts at: 1329 1329
Size: 4307
SmlI C'TyrA_G
Cuts at: 385 958 1462 2377 2869 3131 385
Size: 573 504 915 492 262 1561
Fragments arranged by size:
1561 915 573 504 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4307
SphI G_CATG'C
Cuts at: 1360 1860 1360
Size: 500 3807
SspI AAT'ATT
Cuts at: 891 891
Size: 4307
StyI C'CwwG_G
Cuts at: 209 927 1271 1441 1795 209
Size: 718 344 170 354 2721
Fragments arranged by size:
2721 718 354 344 170
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1075 1890 2023 2482 2691 1075
Size: 815 133 459 209 2691
Fragments arranged by size:
2691 815 459 209 133
TatI w'GTAC_w
Cuts at: 3822 3822
Size: 4307
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3824 3856 3824
Size: 32 4275
Tth111I GACn'n_nGTC
Cuts at: 2104 2104
Size: 4307
XbaI T'CTAG_A
Cuts at: 1338 1338
Size: 4307
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1802 1802
Size: 4307
XmaI C'CCGG_G
Cuts at: 1327 1327
Size: 4307
XmnI GAAnn'nnTTC
Cuts at: 1282 1282
Size: 4307
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PciI PflMI PmeI
PpiI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AvrII BaeI
BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsaXI BsiWI
BsmI BsmBI BsrGI BstXI BstZ17I Bsu36I BtgI ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI NcoI
NdeI NheI NotI NruI NsiI PacI PfoI PmlI
PpuMI PshAI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI ZraI