Restriction map for
pF1KA0464
(Circular) (Six-base) MAPSORT of: pF1KA0464.seq Check: 753 from: 1 to: 4085
pF1KA0464 4085 bp
With 182 enzymes: SgfI *
September 18, 2009 16:59 ..
AccI GT'mk_AC
Cuts at: 1123 1123
Size: 4085
Acc65I G'GTAC_C
Cuts at: 1101 1101
Size: 4085
AclI AA'CG_TT
Cuts at: 3925 3925
Size: 4085
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 462 498 1910 2342 3089 3357 382
Size: 80 36 1412 432 747 268 1110
Fragments arranged by size:
1412 1110 747 432 268 80 36
AflIII A'CryG_T
Cuts at: 1437 2541 3518 1437
Size: 1104 977 2004
Fragments arranged by size:
2004 1104 977
AgeI A'CCGG_T
Cuts at: 2473 2473
Size: 4085
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 644 644
Size: 4085
AlwNI CAG_nnn'CTG
Cuts at: 1390 2957 1390
Size: 1567 2518
ApaLI G'TGCA_C
Cuts at: 2855 2855
Size: 4085
ApoI r'AATT_y
Cuts at: 787 1089 3438 3694 787
Size: 302 2349 256 1178
Fragments arranged by size:
2349 1178 302 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4085
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4085
AvaI C'yCGr_G
Cuts at: 491 1105 491
Size: 614 3471
BamHI G'GATC_C
Cuts at: 158 1110 158
Size: 952 3133
BanI G'GyrC_C
Cuts at: 499 1101 1515 1763 1798 499
Size: 602 414 248 35 2786
Fragments arranged by size:
2786 602 414 248 35
BanII G_rGCy'C
Cuts at: 875 1099 2129 875
Size: 224 1030 2831
Fragments arranged by size:
2831 1030 224
BbeI G_GCGC'C
Cuts at: 1519 1767 1519
Size: 248 3837
BbvCI CC'TCA_GC
Cuts at: 1039 1039
Size: 4085
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4051
BciVI GTATCCnnnnn_n'
Cuts at: 272 1983 2744 4085 272
Size: 1711 761 1341 272
Fragments arranged by size:
1711 1341 761 272
BclI T'GATC_A
Cuts at: 1605 1605
Size: 4085
BglI GCCn_nnn'nGGC
Cuts at: 712 721 712
Size: 9 4076
BglII A'GATC_T
Cuts at: 473 1600 3637 473
Size: 1127 2037 921
Fragments arranged by size:
2037 1127 921
BlpI GC'TnA_GC
Cuts at: 604 1197 604
Size: 593 3492
Bme1580I G_kGCm'C
Cuts at: 149 940 1710 1803 2859 149
Size: 791 770 93 1056 1375
Fragments arranged by size:
1375 1056 791 770 93
BmgBI CAC'GTC
Cuts at: 231 392 231
Size: 161 3924
BmrI ACTGGGnnnn_n'
Cuts at: 1297 1378 1713 3857 1297
Size: 81 335 2144 1525
Fragments arranged by size:
2144 1525 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 732 2452 732
Size: 1720 2365
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 722 911 964 976 722
Size: 189 53 12 3831
Fragments arranged by size:
3831 189 53 12
Bpu10I CC'TnA_GC
Cuts at: 675 1039 675
Size: 364 3721
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1261 2140 2632 2930 1261
Size: 879 492 298 2416
Fragments arranged by size:
2416 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 674 3789 674
Size: 3115 970
BsaAI yAC'GTr
Cuts at: 216 1438 216
Size: 1222 2863
BsaBI GATnn'nnATC
Cuts at: 6 1599 3636 6
Size: 1593 2037 455
Fragments arranged by size:
2037 1593 455
BsaHI Gr'CG_yC
Cuts at: 179 1516 1764 2466 3962 179
Size: 1337 248 702 1496 302
Fragments arranged by size:
1496 1337 702 302 248
BsaWI w'CCGG_w
Cuts at: 1274 1795 2473 2747 2894 3305 1274
Size: 521 678 274 147 411 2054
Fragments arranged by size:
2054 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 338 368 832 862 338
Size: 30 464 30 3561
Fragments arranged by size:
3561 464 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 748 967 979 1027 1036 748
Size: 219 12 48 9 3797
Fragments arranged by size:
3797 219 48 12 9
BseYI C'CCAG_C
Cuts at: 1510 2268 2845 3341 1510
Size: 758 577 496 2254
Fragments arranged by size:
2254 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1627 1673 2881 104
Size: 1523 46 1208 1308
Fragments arranged by size:
1523 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1099 1877 2067 2859 1099
Size: 778 190 792 2325
Fragments arranged by size:
2325 792 778 190
BsmI GAATG_Cn'
Cuts at: 633 633
Size: 4085
Bsp1286I G_dGCh'C
Cuts at: 149 875 940 1099 1710 1803 1877 2067 2129
Size: 726 65 159 611 93 74 190 62
Cuts at: 2129 2859 149
Size: 730 1375
Fragments arranged by size:
1375 730 726 611 190 159 93 74
65 62
BspEI T'CCGG_A
Cuts at: 3305 3305
Size: 4085
BspHI T'CATG_A
Cuts at: 453 3261 453
Size: 2808 1277
BspMI ACCTGCnnnn'nnnn_
Cuts at: 161 1135 1349 1651 2032 161
Size: 974 214 302 381 2214
Fragments arranged by size:
2214 974 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2377 2431 3915 4080 50
Size: 2327 54 1484 165 55
Fragments arranged by size:
2327 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1997 1997
Size: 4085
BsrFI r'CCGG_y
Cuts at: 2083 2264 2473 2083
Size: 181 209 3695
Fragments arranged by size:
3695 209 181
BssSI C'ACGA_G
Cuts at: 2356 2714 2356
Size: 358 3727
BstAPI GCAn_nnn'nTGC
Cuts at: 153 1462 153
Size: 1309 2776
BstBI TT'CG_AA
Cuts at: 2446 2446
Size: 4085
BstXI CCAn_nnnn'nTGG
Cuts at: 646 700 1073 646
Size: 54 373 3658
Fragments arranged by size:
3658 373 54
BstYI r'GATC_y
Cuts at: 1 158 437 473 1110 1577 1600 1935 2181
Size: 157 279 36 637 467 23 335 246
Cuts at: 2181 3193 3279 3291 3637 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 637 467 449 346 335 279 246
157 86 36 23 12
BtgI C'CryG_G
Cuts at: 719 719
Size: 4085
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2035 2216 2379 2035
Size: 181 163 3741
Fragments arranged by size:
3741 181 163
BtsI GCAGTG_nn'
Cuts at: 725 1328 725
Size: 603 3482
ClaI AT'CG_AT
Cuts at: 746 746
Size: 4085
DraI TTT'AAA
Cuts at: 294 531 1084 294
Size: 237 553 3295
Fragments arranged by size:
3295 553 237
DrdI GACnn_nn'nnGTC
Cuts at: 951 1791 2649 951
Size: 840 858 2387
Fragments arranged by size:
2387 858 840
EaeI y'GGCC_r
Cuts at: 636 722 1670 1844 2235 2262 3607 636
Size: 86 948 174 391 27 1345 1114
Fragments arranged by size:
1345 1114 948 391 174 86 27
EagI C'GGCC_G
Cuts at: 1670 1670
Size: 4085
EarI CTCTTCn'nnn_
Cuts at: 538 2108 2318 538
Size: 1570 210 2305
Fragments arranged by size:
2305 1570 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2293 2603 2749 3895 2293
Size: 310 146 1146 2483
Fragments arranged by size:
2483 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1097 1097
Size: 4085
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 462 498 722 911 964 976 1910 2342
Size: 80 36 224 189 53 12 934 432
Cuts at: 2342 3089 3357 382
Size: 747 268 1110
Fragments arranged by size:
1110 934 747 432 268 224 189 80
53 36 12
EcoNI CCTnn'n_nnAGG
Cuts at: 891 891
Size: 4085
EcoO109I rG'GnC_Cy
Cuts at: 423 713 759 1224 423
Size: 290 46 465 3284
Fragments arranged by size:
3284 465 290 46
EcoRI G'AATT_C
Cuts at: 787 1089 787
Size: 302 3783
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2104 2104
Size: 4085
FspI TGC'GCA
Cuts at: 1866 1866
Size: 4085
HaeII r_GCGC'y
Cuts at: 1519 1767 2789 3768 1519
Size: 248 1022 979 1836
Fragments arranged by size:
1836 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1927 1957 1959 1989 1927
Size: 30 2 30 4023
Fragments arranged by size:
4023 30 30 2
HincII GTy'rAC
Cuts at: 1124 1124
Size: 4085
KasI G'GCGC_C
Cuts at: 1515 1763 1515
Size: 248 3837
KpnI G_GTAC'C
Cuts at: 1105 1105
Size: 4085
MluI A'CGCG_T
Cuts at: 3518 3518
Size: 4085
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2756 2940 2756
Size: 184 3901
MscI TGG'CCA
Cuts at: 638 1846 638
Size: 1208 2877
MslI CAynn'nnrTG
Cuts at: 1071 2074 2201 1071
Size: 1003 127 2955
Fragments arranged by size:
2955 1003 127
MspA1I CmG'CkG
Cuts at: 951 1196 1510 1870 2883 3128 951
Size: 245 314 360 1013 245 1908
Fragments arranged by size:
1908 1013 360 314 245 245
NaeI GCC'GGC
Cuts at: 2266 2266
Size: 4085
NarI GG'CG_CC
Cuts at: 1516 1764 1516
Size: 248 3837
NgoMIV G'CCGG_C
Cuts at: 2264 2264
Size: 4085
NspI r_CATG'y
Cuts at: 1138 1638 2545 1138
Size: 500 907 2678
Fragments arranged by size:
2678 907 500
PciI A'CATG_T
Cuts at: 2541 2541
Size: 4085
PfoI T'CCnGG_A
Cuts at: 782 782
Size: 4085
PmeI GTTT'AAAC
Cuts at: 1084 1084
Size: 4085
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3224 3256 3224
Size: 32 4053
PpuMI rG'GwC_Cy
Cuts at: 423 759 423
Size: 336 3749
PstI C_TGCA'G
Cuts at: 614 732 1132 1817 614
Size: 118 400 685 2882
Fragments arranged by size:
2882 685 400 118
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4085
PvuII CAG'CTG
Cuts at: 1510 1870 1510
Size: 360 3725
RsrII CG'GwC_CG
Cuts at: 2280 2280
Size: 4085
SacI G_AGCT'C
Cuts at: 1099 1099
Size: 4085
SalI G'TCGA_C
Cuts at: 1122 1122
Size: 4085
SapI GCTCTTCn'nnn_
Cuts at: 538 2108 2318 538
Size: 1570 210 2305
Fragments arranged by size:
2305 1570 210
SbfI CC_TGCA'GG
Cuts at: 1132 1132
Size: 4085
ScaI AGT'ACT
Cuts at: 3602 3602
Size: 4085
SfcI C'TryA_G
Cuts at: 33 432 610 728 818 1128 1813 2806 2997
Size: 399 178 118 90 310 685 993 191
Cuts at: 2997 33
Size: 1121
Fragments arranged by size:
1121 993 685 399 310 191 178 118
90
SfiI GGCCn_nnn'nGGCC
Cuts at: 721 721
Size: 4085
SfoI GGC'GCC
Cuts at: 1517 1765 1517
Size: 248 3837
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4085
SmaI CCC'GGG
Cuts at: 1107 1107
Size: 4085
SmlI C'TyrA_G
Cuts at: 491 1240 2155 2647 2909 491
Size: 749 915 492 262 1667
Fragments arranged by size:
1667 915 749 492 262
SnaBI TAC'GTA
Cuts at: 216 216
Size: 4085
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4085
SphI G_CATG'C
Cuts at: 1138 1638 1138
Size: 500 3585
StuI AGG'CCT
Cuts at: 583 932 583
Size: 349 3736
StyI C'CwwG_G
Cuts at: 235 851 1219 1573 235
Size: 616 368 354 2747
Fragments arranged by size:
2747 616 368 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 906 1668 1801 2260 2469 906
Size: 762 133 459 209 2522
Fragments arranged by size:
2522 762 459 209 133
TatI w'GTAC_w
Cuts at: 125 3600 125
Size: 3475 610
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 338 370 376 408 832 864 3602 3634 338
Size: 32 6 32 424 32 2738 32 789
Fragments arranged by size:
2738 789 424 32 32 32 32 6
Tth111I GACn'n_nGTC
Cuts at: 1882 1882
Size: 4085
XbaI T'CTAG_A
Cuts at: 810 1116 810
Size: 306 3779
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 249 1580 249
Size: 1331 2754
XhoI C'TCGA_G
Cuts at: 491 491
Size: 4085
XmaI C'CCGG_G
Cuts at: 1105 1105
Size: 4085
XmnI GAAnn'nnTTC
Cuts at: 475 475
Size: 4085
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI ClaI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PfoI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BfrBI BmtI BsgI BsiWI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SgrAI SrfI SspI SwaI ZraI