Restriction map for
pF1KA0471
(Circular) (Six-base) MAPSORT of: pF1KA0471.seq Check: 5240 from: 1 to: 4223
pF1KA0471 4223 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 1261 1261
Size: 4223
Acc65I G'GTAC_C
Cuts at: 1239 1239
Size: 4223
AclI AA'CG_TT
Cuts at: 4063 4063
Size: 4223
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 695 711 2048 2480 3227 3495 442
Size: 253 16 1337 432 747 268 1170
Fragments arranged by size:
1337 1170 747 432 268 253 16
AflII C'TTAA_G
Cuts at: 988 988
Size: 4223
AflIII A'CryG_T
Cuts at: 1575 2679 3656 1575
Size: 1104 977 2142
Fragments arranged by size:
2142 1104 977
AgeI A'CCGG_T
Cuts at: 1188 2611 1188
Size: 1423 2800
AleI CACnn'nnGTG
Cuts at: 896 896
Size: 4223
AlwNI CAG_nnn'CTG
Cuts at: 663 1528 3095 663
Size: 865 1567 1791
Fragments arranged by size:
1791 1567 865
ApaLI G'TGCA_C
Cuts at: 2993 2993
Size: 4223
ApoI r'AATT_y
Cuts at: 1061 1227 3576 3832 1061
Size: 166 2349 256 1452
Fragments arranged by size:
2349 1452 256 166
AseI AT'TA_AT
Cuts at: 20 20
Size: 4223
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4223
AvaI C'yCGr_G
Cuts at: 1243 1243
Size: 4223
BamHI G'GATC_C
Cuts at: 1248 1248
Size: 4223
BanI G'GyrC_C
Cuts at: 120 1165 1239 1653 1901 1936 120
Size: 1045 74 414 248 35 2407
Fragments arranged by size:
2407 1045 414 248 74 35
BanII G_rGCy'C
Cuts at: 644 1092 1237 2267 644
Size: 448 145 1030 2600
Fragments arranged by size:
2600 1030 448 145
BbeI G_GCGC'C
Cuts at: 1657 1905 1657
Size: 248 3975
BbsI GAAGACnn'nnnn_
Cuts at: 293 381 482 620 717 772 293
Size: 88 101 138 97 55 3744
Fragments arranged by size:
3744 138 101 97 88 55
BciVI GTATCCnnnnn_n'
Cuts at: 2121 2882 4223 2121
Size: 761 1341 2121
Fragments arranged by size:
2121 1341 761
BclI T'GATC_A
Cuts at: 1743 1743
Size: 4223
BglII A'GATC_T
Cuts at: 797 1738 3775 797
Size: 941 2037 1245
Fragments arranged by size:
2037 1245 941
BlpI GC'TnA_GC
Cuts at: 143 1335 143
Size: 1192 3031
Bme1580I G_kGCm'C
Cuts at: 125 1168 1848 1941 2997 125
Size: 1043 680 93 1056 1351
Fragments arranged by size:
1351 1056 1043 680 93
BmrI ACTGGGnnnn_n'
Cuts at: 1435 1516 1851 3995 1435
Size: 81 335 2144 1663
Fragments arranged by size:
2144 1663 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 999 2590 999
Size: 1591 2632
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 844 844
Size: 4223
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1399 2278 2770 3068 1399
Size: 879 492 298 2554
Fragments arranged by size:
2554 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3927 3927
Size: 4223
BsaAI yAC'GTr
Cuts at: 1576 1576
Size: 4223
BsaBI GATnn'nnATC
Cuts at: 6 1737 3774 6
Size: 1731 2037 455
Fragments arranged by size:
2037 1731 455
BsaHI Gr'CG_yC
Cuts at: 1654 1902 2604 4100 1654
Size: 248 702 1496 1777
Fragments arranged by size:
1777 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1188 1412 1933 2611 2885 3032 3443 1188
Size: 224 521 678 274 147 411 1968
Fragments arranged by size:
1968 678 521 411 274 224 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1039 1069 1039
Size: 30 4193
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 204 694 865 1100 204
Size: 490 171 235 3327
Fragments arranged by size:
3327 490 235 171
BseYI C'CCAG_C
Cuts at: 129 135 710 1169 1196 1648 2406 2983 3479
Size: 6 575 459 27 452 758 577 496
Cuts at: 3479 129
Size: 873
Fragments arranged by size:
873 758 577 575 496 459 452 27
6
BsiEI CG_ry'CG
Cuts at: 104 1765 1811 3019 104
Size: 1661 46 1208 1308
Fragments arranged by size:
1661 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 644 1216 1237 2015 2205 2997 644
Size: 572 21 778 190 792 1870
Fragments arranged by size:
1870 792 778 572 190 21
Bsp1286I G_dGCh'C
Cuts at: 125 644 1092 1168 1216 1237 1848 1941 2015
Size: 519 448 76 48 21 611 93 74
Cuts at: 2015 2205 2267 2997 125
Size: 190 62 730 1351
Fragments arranged by size:
1351 730 611 519 448 190 93 76
74 62 48 21
BspEI T'CCGG_A
Cuts at: 3443 3443
Size: 4223
BspHI T'CATG_A
Cuts at: 3399 3399
Size: 4223
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1273 1487 1789 2170 1273
Size: 214 302 381 3326
Fragments arranged by size:
3326 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2515 2569 4053 4218 50
Size: 2465 54 1484 165 55
Fragments arranged by size:
2465 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 779 1174 2135 779
Size: 395 961 2867
Fragments arranged by size:
2867 961 395
BsrFI r'CCGG_y
Cuts at: 1188 2221 2402 2611 1188
Size: 1033 181 209 2800
Fragments arranged by size:
2800 1033 209 181
BssSI C'ACGA_G
Cuts at: 2494 2852 2494
Size: 358 3865
BstAPI GCAn_nnn'nTGC
Cuts at: 893 1600 893
Size: 707 3516
BstBI TT'CG_AA
Cuts at: 2584 2584
Size: 4223
BstEII G'GTnAC_C
Cuts at: 1191 1191
Size: 4223
BstYI r'GATC_y
Cuts at: 1 797 1248 1715 1738 2073 2319 3331 3417
Size: 796 451 467 23 335 246 1012 86
Cuts at: 3417 3429 3775 1
Size: 12 346 449
Fragments arranged by size:
1012 796 467 451 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 1160 107
Size: 1053 3170
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2173 2354 2517 2173
Size: 181 163 3879
Fragments arranged by size:
3879 181 163
BtsI GCAGTG_nn'
Cuts at: 891 903 1466 891
Size: 12 563 3648
Fragments arranged by size:
3648 563 12
DraI TTT'AAA
Cuts at: 320 1222 320
Size: 902 3321
DrdI GACnn_nn'nnGTC
Cuts at: 1929 2787 1929
Size: 858 3365
EaeI y'GGCC_r
Cuts at: 1157 1808 1982 2373 2400 3745 1157
Size: 651 174 391 27 1345 1635
Fragments arranged by size:
1635 1345 651 391 174 27
EagI C'GGCC_G
Cuts at: 1808 1808
Size: 4223
EarI CTCTTCn'nnn_
Cuts at: 671 679 695 2246 2456 671
Size: 8 16 1551 210 2438
Fragments arranged by size:
2438 1551 210 16 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2431 2741 2887 4033 2431
Size: 310 146 1146 2621
Fragments arranged by size:
2621 1146 310 146
EcoICRI GAG'CTC
Cuts at: 642 1235 642
Size: 593 3630
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 695 711 844 2048 2480 3227 3495 442
Size: 253 16 133 1204 432 747 268 1170
Fragments arranged by size:
1204 1170 747 432 268 253 133 16
EcoNI CCTnn'n_nnAGG
Cuts at: 295 295
Size: 4223
EcoO109I rG'GnC_Cy
Cuts at: 1362 1362
Size: 4223
EcoRI G'AATT_C
Cuts at: 1227 1227
Size: 4223
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 661 2242 661
Size: 1581 2642
FspI TGC'GCA
Cuts at: 2004 2004
Size: 4223
HaeII r_GCGC'y
Cuts at: 1657 1905 2927 3906 1657
Size: 248 1022 979 1974
Fragments arranged by size:
1974 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 231 263 2065 2095 2097 2127 231
Size: 32 1802 30 2 30 2327
Fragments arranged by size:
2327 1802 32 30 30 2
HincII GTy'rAC
Cuts at: 1262 1262
Size: 4223
KasI G'GCGC_C
Cuts at: 1653 1901 1653
Size: 248 3975
KpnI G_GTAC'C
Cuts at: 1243 1243
Size: 4223
MluI A'CGCG_T
Cuts at: 3656 3656
Size: 4223
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 515 2894 3078 515
Size: 2379 184 1660
Fragments arranged by size:
2379 1660 184
MscI TGG'CCA
Cuts at: 1984 1984
Size: 4223
MslI CAynn'nnrTG
Cuts at: 896 2212 2339 896
Size: 1316 127 2780
Fragments arranged by size:
2780 1316 127
MspA1I CmG'CkG
Cuts at: 139 465 999 1334 1648 2008 3021 3266 139
Size: 326 534 335 314 360 1013 245 1096
Fragments arranged by size:
1096 1013 534 360 335 326 314 245
NaeI GCC'GGC
Cuts at: 2404 2404
Size: 4223
NarI GG'CG_CC
Cuts at: 1654 1902 1654
Size: 248 3975
NcoI C'CATG_G
Cuts at: 107 107
Size: 4223
NgoMIV G'CCGG_C
Cuts at: 2402 2402
Size: 4223
NspI r_CATG'y
Cuts at: 180 887 1276 1776 2683 180
Size: 707 389 500 907 1720
Fragments arranged by size:
1720 907 707 500 389
PciI A'CATG_T
Cuts at: 2679 2679
Size: 4223
PmeI GTTT'AAAC
Cuts at: 1222 1222
Size: 4223
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3362 3394 3362
Size: 32 4191
PstI C_TGCA'G
Cuts at: 299 898 1040 1270 1955 299
Size: 599 142 230 685 2567
Fragments arranged by size:
2567 685 599 230 142
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4223
PvuII CAG'CTG
Cuts at: 139 465 999 1648 2008 139
Size: 326 534 649 360 2354
Fragments arranged by size:
2354 649 534 360 326
RsrII CG'GwC_CG
Cuts at: 2418 2418
Size: 4223
SacI G_AGCT'C
Cuts at: 644 1237 644
Size: 593 3630
SalI G'TCGA_C
Cuts at: 1260 1260
Size: 4223
SapI GCTCTTCn'nnn_
Cuts at: 2246 2456 2246
Size: 210 4013
SbfI CC_TGCA'GG
Cuts at: 1270 1270
Size: 4223
ScaI AGT'ACT
Cuts at: 3740 3740
Size: 4223
SfcI C'TryA_G
Cuts at: 33 295 770 894 1036 1266 1951 2944 3135
Size: 262 475 124 142 230 685 993 191
Cuts at: 3135 33
Size: 1121
Fragments arranged by size:
1121 993 685 475 262 230 191 142
124
SfoI GGC'GCC
Cuts at: 1655 1903 1655
Size: 248 3975
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4223
SmaI CCC'GGG
Cuts at: 1245 1245
Size: 4223
SmlI C'TyrA_G
Cuts at: 988 1378 2293 2785 3047 988
Size: 390 915 492 262 2164
Fragments arranged by size:
2164 915 492 390 262
SpeI A'CTAG_T
Cuts at: 67 567 67
Size: 500 3723
SphI G_CATG'C
Cuts at: 887 1276 1776 887
Size: 389 500 3334
Fragments arranged by size:
3334 500 389
SspI AAT'ATT
Cuts at: 399 399
Size: 4223
StyI C'CwwG_G
Cuts at: 107 1205 1357 1711 107
Size: 1098 152 354 2619
Fragments arranged by size:
2619 1098 354 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1183 1210 1218 1806 1939 2398 2607 1183
Size: 27 8 588 133 459 209 2799
Fragments arranged by size:
2799 588 459 209 133 27 8
TatI w'GTAC_w
Cuts at: 3738 3738
Size: 4223
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1039 1071 3740 3772 1039
Size: 32 2669 32 1490
Fragments arranged by size:
2669 1490 32 32
Tth111I GACn'n_nGTC
Cuts at: 2020 2020
Size: 4223
XbaI T'CTAG_A
Cuts at: 1254 1254
Size: 4223
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 531 660 1038 1718 531
Size: 129 378 680 3036
Fragments arranged by size:
3036 680 378 129
XmaI C'CCGG_G
Cuts at: 1243 1243
Size: 4223
XmnI GAAnn'nnTTC
Cuts at: 290 290
Size: 4223
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BssHII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI