Restriction map for
pF1KA0472
(Circular) (Six-base) MAPSORT of: pF1KA0472.seq Check: 7176 from: 1 to: 5900
pF1KA0472 5900 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 501 1788 501
Size: 1287 4613
AatII G_ACGT'C
Cuts at: 1436 1436
Size: 5900
AccI GT'mk_AC
Cuts at: 2105 2600 2938 2105
Size: 495 338 5067
Fragments arranged by size:
5067 495 338
Acc65I G'GTAC_C
Cuts at: 2916 2916
Size: 5900
AclI AA'CG_TT
Cuts at: 5740 5740
Size: 5900
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 886 2362 3725 4157 4510 5172 886
Size: 1476 1363 432 353 662 1614
Fragments arranged by size:
1614 1476 1363 662 432 353
AflIII A'CryG_T
Cuts at: 2365 3252 5052 5333 2365
Size: 887 1800 281 2932
Fragments arranged by size:
2932 1800 887 281
AleI CACnn'nnGTG
Cuts at: 1768 1768
Size: 5900
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2311 2492 2311
Size: 181 5719
AlwNI CAG_nnn'CTG
Cuts at: 240 1058 1611 2079 2641 3205 4643 240
Size: 818 553 468 562 564 1438 1497
Fragments arranged by size:
1497 1438 818 564 562 553 468
ApaLI G'TGCA_C
Cuts at: 628 1201 4738 628
Size: 573 3537 1790
Fragments arranged by size:
3537 1790 573
ApoI r'AATT_y
Cuts at: 2197 2612 2904 5253 5509 2197
Size: 415 292 2349 256 2588
Fragments arranged by size:
2588 2349 415 292 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5900
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5900
AvaI C'yCGr_G
Cuts at: 147 590 2920 147
Size: 443 2330 3127
Fragments arranged by size:
3127 2330 443
BamHI G'GATC_C
Cuts at: 2925 2925
Size: 5900
BanI G'GyrC_C
Cuts at: 123 560 1465 2916 3330 3578 3613 123
Size: 437 905 1451 414 248 35 2410
Fragments arranged by size:
2410 1451 905 437 414 248 35
BanII G_rGCy'C
Cuts at: 143 964 1074 1777 2715 2914 3944 143
Size: 821 110 703 938 199 1030 2099
Fragments arranged by size:
2099 1030 938 821 703 199 110
BbeI G_GCGC'C
Cuts at: 564 3334 3582 564
Size: 2770 248 2882
Fragments arranged by size:
2882 2770 248
BbsI GAAGACnn'nnnn_
Cuts at: 2719 2719
Size: 5900
BbvCI CC'TCA_GC
Cuts at: 1834 1975 1834
Size: 141 5759
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1888 1922 1888
Size: 34 5866
BciVI GTATCCnnnnn_n'
Cuts at: 1077 3798 4854 5900 1077
Size: 2721 1056 1046 1077
Fragments arranged by size:
2721 1077 1056 1046
BclI T'GATC_A
Cuts at: 1547 3420 1547
Size: 1873 4027
BglI GCCn_nnn'nGGC
Cuts at: 164 341 1943 164
Size: 177 1602 4121
Fragments arranged by size:
4121 1602 177
BglII A'GATC_T
Cuts at: 1664 1965 3415 5452 1664
Size: 301 1450 2037 2112
Fragments arranged by size:
2112 2037 1450 301
BlpI GC'TnA_GC
Cuts at: 3012 3012
Size: 5900
Bme1580I G_kGCm'C
Cuts at: 632 1205 2145 3525 3618 4742 632
Size: 573 940 1380 93 1124 1790
Fragments arranged by size:
1790 1380 1124 940 573 93
BmrI ACTGGGnnnn_n'
Cuts at: 666 967 2077 2089 2130 3112 3193 3528 5672
Size: 301 1110 12 41 982 81 335 2144
Cuts at: 5672 666
Size: 894
Fragments arranged by size:
2144 1110 982 894 335 301 81 41
12
BmtI G_CTAG'C
Cuts at: 2678 2678
Size: 5900
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 162 2729 4267 162
Size: 2567 1538 1795
Fragments arranged by size:
2567 1795 1538
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 703 751 1633 2147 2268 2380 2809 403
Size: 300 48 882 514 121 112 429 3494
Fragments arranged by size:
3494 882 514 429 300 121 112 48
Bpu10I CC'TnA_GC
Cuts at: 1834 1975 2634 1834
Size: 141 659 5100
Fragments arranged by size:
5100 659 141
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1155 3076 3955 4669 4967 1155
Size: 1921 879 714 298 2088
Fragments arranged by size:
2088 1921 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 241 655 2219 5604 241
Size: 414 1564 3385 537
Fragments arranged by size:
3385 1564 537 414
BsaAI yAC'GTr
Cuts at: 3253 3253
Size: 5900
BsaBI GATnn'nnATC
Cuts at: 6 3414 5451 6
Size: 3408 2037 455
Fragments arranged by size:
3408 2037 455
BsaHI Gr'CG_yC
Cuts at: 561 1433 3331 3579 4281 5777 561
Size: 872 1898 248 702 1496 684
Fragments arranged by size:
1898 1496 872 702 684 248
BsaWI w'CCGG_w
Cuts at: 1874 3089 3610 4288 4699 4846 5120 1874
Size: 1215 521 678 411 147 274 2654
Fragments arranged by size:
2654 1215 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1291 1321 1445 1475 1291
Size: 30 124 30 5716
Fragments arranged by size:
5716 124 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 291 556 688 969 2290 2758 291
Size: 265 132 281 1321 468 3433
Fragments arranged by size:
3433 1321 468 281 265 132
BseYI C'CCAG_C
Cuts at: 531 2347 3325 4083 4748 5156 531
Size: 1816 978 758 665 408 1275
Fragments arranged by size:
1816 1275 978 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 529 2395 529
Size: 1866 4034
BsiEI CG_ry'CG
Cuts at: 104 204 3442 3488 4718 104
Size: 100 3238 46 1230 1286
Fragments arranged by size:
3238 1286 1230 100 46
BsiHKAI G_wGCw'C
Cuts at: 280 632 1141 1205 2300 2914 3692 3882 4742
Size: 352 509 64 1095 614 778 190 860
Cuts at: 4742 280
Size: 1438
Fragments arranged by size:
1438 1095 860 778 614 509 352 190
64
BsmBI CGTCTCn'nnnn_
Cuts at: 150 150
Size: 5900
Bsp1286I G_dGCh'C
Cuts at: 143 280 632 964 1074 1141 1205 1777 2145
Size: 137 352 332 110 67 64 572 368
Cuts at: 2145 2300 2715 2914 3525 3618 3692 3882 3944
Size: 155 415 199 611 93 74 190 62
Cuts at: 3944 4742 143
Size: 798 1301
Fragments arranged by size:
1301 798 611 572 415 368 352 332
199 190 155 137 110 93 74 67
64 62
BspEI T'CCGG_A
Cuts at: 1874 1874
Size: 5900
BspHI T'CATG_A
Cuts at: 2834 4332 2834
Size: 1498 4402
BspMI ACCTGCnnnn'nnnn_
Cuts at: 248 501 1265 1788 2950 3164 3466 3847 248
Size: 253 764 523 1162 214 302 381 2301
Fragments arranged by size:
2301 1162 764 523 381 302 253 214
BsrBI CCG'CTC
Cuts at: 50 2313 4192 4246 5730 5895 50
Size: 2263 1879 54 1484 165 55
Fragments arranged by size:
2263 1879 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2288 3812 2288
Size: 1524 4376
BsrFI r'CCGG_y
Cuts at: 1946 3898 4079 1946
Size: 1952 181 3767
Fragments arranged by size:
3767 1952 181
BssSI C'ACGA_G
Cuts at: 1897 4171 4879 1897
Size: 2274 708 2918
Fragments arranged by size:
2918 2274 708
BstAPI GCAn_nnn'nTGC
Cuts at: 3277 3277
Size: 5900
BstBI TT'CG_AA
Cuts at: 4261 4261
Size: 5900
BstXI CCAn_nnnn'nTGG
Cuts at: 531 993 1278 1791 531
Size: 462 285 513 4640
Fragments arranged by size:
4640 513 462 285
BstYI r'GATC_y
Cuts at: 1 678 1496 1664 1965 2241 2325 2925 3392
Size: 677 818 168 301 276 84 600 467
Cuts at: 3392 3415 3750 3996 4302 4314 4400 5452 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 818 677 600 467 449 335 306
301 276 246 168 86 84 23 12
BstZ17I GTA'TAC
Cuts at: 2106 2106
Size: 5900
Bsu36I CC'TnA_GG
Cuts at: 401 671 1567 2654 401
Size: 270 896 1087 3647
Fragments arranged by size:
3647 1087 896 270
BtgI C'CryG_G
Cuts at: 107 127 2247 2591 107
Size: 20 2120 344 3416
Fragments arranged by size:
3416 2120 344 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 359 3850 4031 4194 359
Size: 3491 181 163 2065
Fragments arranged by size:
3491 2065 181 163
BtsI GCAGTG_nn'
Cuts at: 543 1203 1209 1538 3143 543
Size: 660 6 329 1605 3300
Fragments arranged by size:
3300 1605 660 329 6
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2256 2291 2256
Size: 35 5865
DraI TTT'AAA
Cuts at: 1428 2899 1428
Size: 1471 4429
DraIII CAC_nnn'GTG
Cuts at: 1155 1155
Size: 5900
DrdI GACnn_nn'nnGTC
Cuts at: 1438 3606 4950 1438
Size: 2168 1344 2388
Fragments arranged by size:
2388 2168 1344
EaeI y'GGCC_r
Cuts at: 201 459 1077 1935 3485 3659 4050 4077 5422
Size: 258 618 858 1550 174 391 27 1345
Cuts at: 5422 201
Size: 679
Fragments arranged by size:
1550 1345 858 679 618 391 258 174
27
EagI C'GGCC_G
Cuts at: 201 3485 201
Size: 3284 2616
EarI CTCTTCn'nnn_
Cuts at: 401 1795 2785 3923 4133 401
Size: 1394 990 1138 210 2168
Fragments arranged by size:
2168 1394 1138 990 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 185 4108 4850 4996 5710 185
Size: 3923 742 146 714 375
Fragments arranged by size:
3923 742 714 375 146
EcoICRI GAG'CTC
Cuts at: 2912 2912
Size: 5900
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 703 751 886 1633 2147 2268 2362 2380
Size: 300 48 135 747 514 121 94 18
Cuts at: 2380 2809 3725 4157 4510 5172 403
Size: 429 916 432 353 662 1131
Fragments arranged by size:
1131 916 747 662 514 432 429 353
300 135 121 94 48 18
EcoNI CCTnn'n_nnAGG
Cuts at: 460 984 2790 460
Size: 524 1806 3570
Fragments arranged by size:
3570 1806 524
EcoO109I rG'GnC_Cy
Cuts at: 151 1188 1254 3039 151
Size: 1037 66 1785 3012
Fragments arranged by size:
3012 1785 1037 66
EcoRI G'AATT_C
Cuts at: 2612 2904 2612
Size: 292 5608
EcoRV GAT'ATC
Cuts at: 2439 2439
Size: 5900
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2048 3919 2048
Size: 1871 4029
FspI TGC'GCA
Cuts at: 3681 3681
Size: 5900
HaeII r_GCGC'y
Cuts at: 564 607 1841 3334 3582 4812 5583 564
Size: 43 1234 1493 248 1230 771 881
Fragments arranged by size:
1493 1234 1230 881 771 248 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 573 605 862 894 1038 1070 3742 3772 3774
Size: 32 257 32 144 32 2672 30 2
Cuts at: 3774 3804 573
Size: 30 2669
Fragments arranged by size:
2672 2669 257 144 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2939 2939
Size: 5900
HindIII A'AGCT_T
Cuts at: 553 1575 553
Size: 1022 4878
KasI G'GCGC_C
Cuts at: 560 3330 3578 560
Size: 2770 248 2882
Fragments arranged by size:
2882 2770 248
KpnI G_GTAC'C
Cuts at: 2920 2920
Size: 5900
MluI A'CGCG_T
Cuts at: 5333 5333
Size: 5900
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1515 1563 4659 4843 1515
Size: 48 3096 184 2572
Fragments arranged by size:
3096 2572 184 48
MscI TGG'CCA
Cuts at: 1079 1937 3661 1079
Size: 858 1724 3318
Fragments arranged by size:
3318 1724 858
MslI CAynn'nnrTG
Cuts at: 869 1105 1768 2180 2293 3889 4016 869
Size: 236 663 412 113 1596 127 2753
Fragments arranged by size:
2753 1596 663 412 236 127 113
MspA1I CmG'CkG
Cuts at: 486 1251 1926 3011 3325 3685 4469 4714 486
Size: 765 675 1085 314 360 784 245 1672
Fragments arranged by size:
1672 1085 784 765 675 360 314 245
NaeI GCC'GGC
Cuts at: 4081 4081
Size: 5900
NarI GG'CG_CC
Cuts at: 561 3331 3579 561
Size: 2770 248 2882
Fragments arranged by size:
2882 2770 248
NcoI C'CATG_G
Cuts at: 107 127 2247 107
Size: 20 2120 3760
Fragments arranged by size:
3760 2120 20
NdeI CA'TA_TG
Cuts at: 2552 2552
Size: 5900
NgoMIV G'CCGG_C
Cuts at: 4079 4079
Size: 5900
NheI G'CTAG_C
Cuts at: 2674 2674
Size: 5900
NspI r_CATG'y
Cuts at: 1104 1248 2953 3453 5056 1104
Size: 144 1705 500 1603 1948
Fragments arranged by size:
1948 1705 1603 500 144
PciI A'CATG_T
Cuts at: 5052 5052
Size: 5900
PfoI T'CCnGG_A
Cuts at: 1499 1499
Size: 5900
PmeI GTTT'AAAC
Cuts at: 2899 2899
Size: 5900
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4346 4378 4346
Size: 32 5868
PpuMI rG'GwC_Cy
Cuts at: 151 1254 151
Size: 1103 4797
PshAI GACnn'nnGTC
Cuts at: 592 592
Size: 5900
PstI C_TGCA'G
Cuts at: 342 368 1262 1607 2947 3632 342
Size: 26 894 345 1340 685 2610
Fragments arranged by size:
2610 1340 894 685 345 26
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5900
PvuII CAG'CTG
Cuts at: 486 1926 3325 3685 486
Size: 1440 1399 360 2701
Fragments arranged by size:
2701 1440 1399 360
RsrII CG'GwC_CG
Cuts at: 4095 4095
Size: 5900
SacI G_AGCT'C
Cuts at: 2914 2914
Size: 5900
SalI G'TCGA_C
Cuts at: 2937 2937
Size: 5900
SanDI GG'GwC_CC
Cuts at: 151 151
Size: 5900
SapI GCTCTTCn'nnn_
Cuts at: 3923 4133 3923
Size: 210 5690
SbfI CC_TGCA'GG
Cuts at: 342 2947 342
Size: 2605 3295
ScaI AGT'ACT
Cuts at: 5417 5417
Size: 5900
SexAI A'CCwGG_T
Cuts at: 1151 1196 1151
Size: 45 5855
SfcI C'TryA_G
Cuts at: 33 338 364 1258 1603 2943 3628 4596 4787
Size: 305 26 894 345 1340 685 968 191
Cuts at: 4787 33
Size: 1146
Fragments arranged by size:
1340 1146 968 894 685 345 305 191
26
SfiI GGCCn_nnn'nGGCC
Cuts at: 1943 1943
Size: 5900
SfoI GGC'GCC
Cuts at: 562 3332 3580 562
Size: 2770 248 2882
Fragments arranged by size:
2882 2770 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5900
SmaI CCC'GGG
Cuts at: 2922 2922
Size: 5900
SmlI C'TyrA_G
Cuts at: 1134 3055 3970 4684 4946 1134
Size: 1921 915 714 262 2088
Fragments arranged by size:
2088 1921 915 714 262
SpeI A'CTAG_T
Cuts at: 67 624 67
Size: 557 5343
SphI G_CATG'C
Cuts at: 2953 3453 2953
Size: 500 5400
SspI AAT'ATT
Cuts at: 1329 1329
Size: 5900
StuI AGG'CCT
Cuts at: 794 2796 794
Size: 2002 3898
StyI C'CwwG_G
Cuts at: 107 127 789 1184 2247 3034 3388 107
Size: 20 662 395 1063 787 354 2619
Fragments arranged by size:
2619 1063 787 662 395 354 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 808 1758 3483 3616 4075 4284 808
Size: 950 1725 133 459 209 2424
Fragments arranged by size:
2424 1725 950 459 209 133
TatI w'GTAC_w
Cuts at: 5415 5415
Size: 5900
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5417 5449 5417
Size: 32 5868
Tth111I GACn'n_nGTC
Cuts at: 3697 3697
Size: 5900
XbaI T'CTAG_A
Cuts at: 2931 2931
Size: 5900
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 528 3395 528
Size: 2867 3033
XmaI C'CCGG_G
Cuts at: 2920 2920
Size: 5900
XmnI GAAnn'nnTTC
Cuts at: 259 835 2726 259
Size: 576 1891 3433
Fragments arranged by size:
3433 1891 576
ZraI GAC'GTC
Cuts at: 1434 1434
Size: 5900
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NspI PciI PfoI PmeI PpiI PpuMI PshAI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AloI ApaI AscI AvrII
BaeI BfrBI BmgBI BsiWI BsmI BsrGI BssHII BstEII
ClaI FseI FspAI HpaI MfeI NotI NruI NsiI
PacI PasI PflMI PmlI PsiI PspOMI PspXI PsrI
SacII SgrAI SnaBI SrfI SwaI XhoI