Restriction map for
pF1KA0473
(Circular) (Six-base) MAPSORT of: pF1KA0473.seq Check: 7079 from: 1 to: 5852
pF1KA0473 5852 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2688 2688
Size: 5852
AccI GT'mk_AC
Cuts at: 431 1771 2890 431
Size: 1340 1119 3393
Fragments arranged by size:
3393 1340 1119
Acc65I G'GTAC_C
Cuts at: 2868 2868
Size: 5852
AclI AA'CG_TT
Cuts at: 5692 5692
Size: 5852
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1060 3677 4109 4856 5124 1060
Size: 2617 432 747 268 1788
Fragments arranged by size:
2617 1788 747 432 268
AflIII A'CryG_T
Cuts at: 3204 4308 5285 3204
Size: 1104 977 3771
Fragments arranged by size:
3771 1104 977
AgeI A'CCGG_T
Cuts at: 4240 4240
Size: 5852
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1529 2300 1529
Size: 771 5081
AlwNI CAG_nnn'CTG
Cuts at: 2133 3157 4724 2133
Size: 1024 1567 3261
Fragments arranged by size:
3261 1567 1024
ApaLI G'TGCA_C
Cuts at: 2734 4622 2734
Size: 1888 3964
ApoI r'AATT_y
Cuts at: 2815 2856 5205 5461 2815
Size: 41 2349 256 3206
Fragments arranged by size:
3206 2349 256 41
AseI AT'TA_AT
Cuts at: 20 20
Size: 5852
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5852
AvaI C'yCGr_G
Cuts at: 2872 2872
Size: 5852
BamHI G'GATC_C
Cuts at: 2089 2136 2553 2877 2089
Size: 47 417 324 5064
Fragments arranged by size:
5064 417 324 47
BanI G'GyrC_C
Cuts at: 130 1840 2284 2868 3282 3530 3565 130
Size: 1710 444 584 414 248 35 2417
Fragments arranged by size:
2417 1710 584 444 414 248 35
BanII G_rGCy'C
Cuts at: 155 2613 2798 2866 3896 155
Size: 2458 185 68 1030 2111
Fragments arranged by size:
2458 2111 1030 185 68
BbeI G_GCGC'C
Cuts at: 3286 3534 3286
Size: 248 5604
BbsI GAAGACnn'nnnn_
Cuts at: 1442 2462 1442
Size: 1020 4832
BbvCI CC'TCA_GC
Cuts at: 1169 2318 1169
Size: 1149 4703
BciVI GTATCCnnnnn_n'
Cuts at: 3750 4511 5852 3750
Size: 761 1341 3750
Fragments arranged by size:
3750 1341 761
BclI T'GATC_A
Cuts at: 3372 3372
Size: 5852
BfrBI ATG'CAT
Cuts at: 1144 2628 1144
Size: 1484 4368
BglI GCCn_nnn'nGGC
Cuts at: 504 504
Size: 5852
BglII A'GATC_T
Cuts at: 3367 5404 3367
Size: 2037 3815
BlpI GC'TnA_GC
Cuts at: 2964 2964
Size: 5852
Bme1580I G_kGCm'C
Cuts at: 2738 3477 3570 4626 2738
Size: 739 93 1056 3964
Fragments arranged by size:
3964 1056 739 93
BmgBI CAC'GTC
Cuts at: 993 993
Size: 5852
BmrI ACTGGGnnnn_n'
Cuts at: 509 1770 2762 3064 3145 3480 5624 509
Size: 1261 992 302 81 335 2144 737
Fragments arranged by size:
2144 1261 992 737 335 302 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4219 4219
Size: 5852
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 125 303 1331 1924 2036 125
Size: 178 1028 593 112 3941
Fragments arranged by size:
3941 1028 593 178 112
Bpu10I CC'TnA_GC
Cuts at: 1046 1169 2312 2318 1046
Size: 123 1143 6 4580
Fragments arranged by size:
4580 1143 123 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1035 3028 3907 4399 4697 1035
Size: 1993 879 492 298 2190
Fragments arranged by size:
2190 1993 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 175 486 2644 5556 175
Size: 311 2158 2912 471
Fragments arranged by size:
2912 2158 471 311
BsaAI yAC'GTr
Cuts at: 3205 3205
Size: 5852
BsaBI GATnn'nnATC
Cuts at: 6 1265 3366 5403 6
Size: 1259 2101 2037 455
Fragments arranged by size:
2101 2037 1259 455
BsaHI Gr'CG_yC
Cuts at: 2245 3283 3531 4233 5729 2245
Size: 1038 248 702 1496 2368
Fragments arranged by size:
2368 1496 1038 702 248
BsaWI w'CCGG_w
Cuts at: 2086 3041 3562 4240 4514 4661 5072 2086
Size: 955 521 678 274 147 411 2866
Fragments arranged by size:
2866 955 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 980 1010 2641 2671 980
Size: 30 1631 30 4161
Fragments arranged by size:
4161 1631 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1646 2328 1646
Size: 682 5170
BseYI C'CCAG_C
Cuts at: 154 675 1270 1549 1893 2252 2323 2644 3277
Size: 521 595 279 344 359 71 321 633
Cuts at: 3277 4035 4612 5108 154
Size: 758 577 496 898
Fragments arranged by size:
898 758 633 595 577 521 496 359
344 321 279 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 215 505 1236 1724 215
Size: 290 731 488 4343
Fragments arranged by size:
4343 731 488 290
BsiEI CG_ry'CG
Cuts at: 104 3394 3440 4648 104
Size: 3290 46 1208 1308
Fragments arranged by size:
3290 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1866 2738 2798 2866 3644 3834 4626 1866
Size: 872 60 68 778 190 792 3092
Fragments arranged by size:
3092 872 792 778 190 68 60
BsmI GAATG_Cn'
Cuts at: 493 493
Size: 5852
BsmBI CGTCTCn'nnnn_
Cuts at: 983 1820 2252 983
Size: 837 432 4583
Fragments arranged by size:
4583 837 432
Bsp1286I G_dGCh'C
Cuts at: 155 1866 2613 2738 2798 2866 3477 3570 3644
Size: 1711 747 125 60 68 611 93 74
Cuts at: 3644 3834 3896 4626 155
Size: 190 62 730 1381
Fragments arranged by size:
1711 1381 747 730 611 190 125 93
74 68 62 60
BspEI T'CCGG_A
Cuts at: 5072 5072
Size: 5852
BspHI T'CATG_A
Cuts at: 5028 5028
Size: 5852
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1773 2688 2902 3116 3418 3799 1773
Size: 915 214 214 302 381 3826
Fragments arranged by size:
3826 915 381 302 214 214
BsrBI CCG'CTC
Cuts at: 50 1793 4144 4198 5682 5847 50
Size: 1743 2351 54 1484 165 55
Fragments arranged by size:
2351 1743 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1518 1631 3764 1518
Size: 113 2133 3606
Fragments arranged by size:
3606 2133 113
BsrFI r'CCGG_y
Cuts at: 1034 3850 4031 4240 1034
Size: 2816 181 209 2646
Fragments arranged by size:
2816 2646 209 181
BsrGI T'GTAC_A
Cuts at: 2711 2711
Size: 5852
BssSI C'ACGA_G
Cuts at: 4123 4481 4123
Size: 358 5494
BstAPI GCAn_nnn'nTGC
Cuts at: 3229 3229
Size: 5852
BstBI TT'CG_AA
Cuts at: 390 4213 390
Size: 3823 2029
BstEII G'GTnAC_C
Cuts at: 1050 1050
Size: 5852
BstXI CCAn_nnnn'nTGG
Cuts at: 2550 2550
Size: 5852
BstYI r'GATC_y
Cuts at: 1 2089 2136 2553 2877 3344 3367 3702 3948
Size: 2088 47 417 324 467 23 335 246
Cuts at: 3948 4960 5046 5058 5404 1
Size: 1012 86 12 346 449
Fragments arranged by size:
2088 1012 467 449 417 346 335 324
246 86 47 23 12
BstZ17I GTA'TAC
Cuts at: 432 432
Size: 5852
Bsu36I CC'TnA_GG
Cuts at: 1317 1589 1317
Size: 272 5580
BtgI C'CryG_G
Cuts at: 1237 1237
Size: 5852
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3802 3983 4146 3802
Size: 181 163 5508
Fragments arranged by size:
5508 181 163
BtsI GCAGTG_nn'
Cuts at: 594 3095 594
Size: 2501 3351
DraI TTT'AAA
Cuts at: 1118 2851 1118
Size: 1733 4119
DrdI GACnn_nn'nnGTC
Cuts at: 3558 4416 3558
Size: 858 4994
EaeI y'GGCC_r
Cuts at: 2540 3437 3611 4002 4029 5374 2540
Size: 897 174 391 27 1345 3018
Fragments arranged by size:
3018 1345 897 391 174 27
EagI C'GGCC_G
Cuts at: 3437 3437
Size: 5852
EarI CTCTTCn'nnn_
Cuts at: 1285 1290 3875 4085 1285
Size: 5 2585 210 3052
Fragments arranged by size:
3052 2585 210 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1634 4060 4370 4516 5662 1634
Size: 2426 310 146 1146 1824
Fragments arranged by size:
2426 1824 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2796 2864 2796
Size: 68 5784
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 125 303 1060 1331 1924 2036 3677 4109 4856
Size: 178 757 271 593 112 1641 432 747
Cuts at: 4856 5124 125
Size: 268 853
Fragments arranged by size:
1641 853 757 747 593 432 271 268
178 112
EcoO109I rG'GnC_Cy
Cuts at: 2503 2991 2503
Size: 488 5364
EcoRI G'AATT_C
Cuts at: 2856 2856
Size: 5852
EcoRV GAT'ATC
Cuts at: 1341 1341
Size: 5852
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 243 1973 3871 243
Size: 1730 1898 2224
Fragments arranged by size:
2224 1898 1730
FspI TGC'GCA
Cuts at: 3633 3633
Size: 5852
HaeII r_GCGC'y
Cuts at: 3286 3534 4556 5535 3286
Size: 248 1022 979 3603
Fragments arranged by size:
3603 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1396 1428 2121 2153 2449 2481 2794 2826 3694
Size: 32 693 32 296 32 313 32 868
Cuts at: 3694 3724 3726 3756 1396
Size: 30 2 30 3492
Fragments arranged by size:
3492 868 693 313 296 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 357 1022 1781 2891 357
Size: 665 759 1110 3318
Fragments arranged by size:
3318 1110 759 665
HindIII A'AGCT_T
Cuts at: 393 393
Size: 5852
KasI G'GCGC_C
Cuts at: 3282 3530 3282
Size: 248 5604
KpnI G_GTAC'C
Cuts at: 2872 2872
Size: 5852
MluI A'CGCG_T
Cuts at: 5285 5285
Size: 5852
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 523 1843 4523 4707 523
Size: 1320 2680 184 1668
Fragments arranged by size:
2680 1668 1320 184
MscI TGG'CCA
Cuts at: 2542 3613 2542
Size: 1071 4781
MslI CAynn'nnrTG
Cuts at: 979 1511 1757 3841 3968 979
Size: 532 246 2084 127 2863
Fragments arranged by size:
2863 2084 532 246 127
MspA1I CmG'CkG
Cuts at: 1651 1708 2071 2446 2963 3277 3637 4650 4895
Size: 57 363 375 517 314 360 1013 245
Cuts at: 4895 1651
Size: 2608
Fragments arranged by size:
2608 1013 517 375 363 360 314 245
57
NaeI GCC'GGC
Cuts at: 1036 4033 1036
Size: 2997 2855
NarI GG'CG_CC
Cuts at: 3283 3531 3283
Size: 248 5604
NcoI C'CATG_G
Cuts at: 1237 1237
Size: 5852
NgoMIV G'CCGG_C
Cuts at: 1034 4031 1034
Size: 2997 2855
NsiI A_TGCA'T
Cuts at: 1146 2630 1146
Size: 1484 4368
NspI r_CATG'y
Cuts at: 1148 1975 2035 2331 2905 3405 4312 1148
Size: 827 60 296 574 500 907 2688
Fragments arranged by size:
2688 907 827 574 500 296 60
PciI A'CATG_T
Cuts at: 4308 4308
Size: 5852
PflMI CCAn_nnn'nTGG
Cuts at: 600 712 2133 2259 600
Size: 112 1421 126 4193
Fragments arranged by size:
4193 1421 126 112
PmeI GTTT'AAAC
Cuts at: 2851 2851
Size: 5852
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4991 5023 4991
Size: 32 5820
PshAI GACnn'nnGTC
Cuts at: 1767 1767
Size: 5852
PstI C_TGCA'G
Cuts at: 569 2899 3584 569
Size: 2330 685 2837
Fragments arranged by size:
2837 2330 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5852
PvuII CAG'CTG
Cuts at: 1708 2071 2446 3277 3637 1708
Size: 363 375 831 360 3923
Fragments arranged by size:
3923 831 375 363 360
RsrII CG'GwC_CG
Cuts at: 4047 4047
Size: 5852
SacI G_AGCT'C
Cuts at: 2798 2866 2798
Size: 68 5784
SalI G'TCGA_C
Cuts at: 2889 2889
Size: 5852
SapI GCTCTTCn'nnn_
Cuts at: 3875 4085 3875
Size: 210 5642
SbfI CC_TGCA'GG
Cuts at: 2899 2899
Size: 5852
ScaI AGT'ACT
Cuts at: 5369 5369
Size: 5852
SexAI A'CCwGG_T
Cuts at: 2681 2681
Size: 5852
SfcI C'TryA_G
Cuts at: 33 565 1039 1201 2895 3580 4573 4764 33
Size: 532 474 162 1694 685 993 191 1121
Fragments arranged by size:
1694 1121 993 685 532 474 191 162
SfoI GGC'GCC
Cuts at: 3284 3532 3284
Size: 248 5604
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5852
SmaI CCC'GGG
Cuts at: 2874 2874
Size: 5852
SmlI C'TyrA_G
Cuts at: 1014 3007 3922 4414 4676 1014
Size: 1993 915 492 262 2190
Fragments arranged by size:
2190 1993 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5852
SphI G_CATG'C
Cuts at: 2035 2331 2905 3405 2035
Size: 296 574 500 4482
Fragments arranged by size:
4482 574 500 296
StyI C'CwwG_G
Cuts at: 1237 2151 2543 2826 2986 3340 1237
Size: 914 392 283 160 354 3749
Fragments arranged by size:
3749 914 392 354 283 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1732 2125 2197 2251 2753 3435 3568 4027 4236
Size: 393 72 54 502 682 133 459 209
Cuts at: 4236 1732
Size: 3348
Fragments arranged by size:
3348 682 502 459 393 209 133 72
54
TatI w'GTAC_w
Cuts at: 876 1968 2711 5367 876
Size: 1092 743 2656 1361
Fragments arranged by size:
2656 1361 1092 743
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 807 839 1052 1084 1938 1970 2641 2673 5369
Size: 32 213 32 854 32 671 32 2696
Cuts at: 5369 5401 807
Size: 32 1258
Fragments arranged by size:
2696 1258 854 671 213 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 3649 3649
Size: 5852
XbaI T'CTAG_A
Cuts at: 256 2883 256
Size: 2627 3225
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 601 2260 2401 3347 601
Size: 1659 141 946 3106
Fragments arranged by size:
3106 1659 946 141
XmaI C'CCGG_G
Cuts at: 2872 2872
Size: 5852
XmnI GAAnn'nnTTC
Cuts at: 1423 1956 1423
Size: 533 5319
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI
PflMI PmeI PpiI PshAI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BcgI BmtI BsiWI BssHII ClaI CspCI
DraIII EcoNI FseI FspAI HpaI MfeI NdeI NheI
NotI NruI PacI PasI PfoI PmlI PpuMI PsiI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI