Restriction map for
pF1KA0476
(Circular) (Six-base) MAPSORT of: pF1KA0476.seq Check: 6791 from: 1 to: 7601
pF1KA0476 7601 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2832 4388 2832
Size: 1556 6045
AccI GT'mk_AC
Cuts at: 1583 4639 1583
Size: 3056 4545
Acc65I G'GTAC_C
Cuts at: 4617 4617
Size: 7601
AclI AA'CG_TT
Cuts at: 1187 7441 1187
Size: 6254 1347
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2902 5426 5858 6605 6873 2902
Size: 2524 432 747 268 3630
Fragments arranged by size:
3630 2524 747 432 268
AflIII A'CryG_T
Cuts at: 4953 6057 7034 4953
Size: 1104 977 5520
Fragments arranged by size:
5520 1104 977
AgeI A'CCGG_T
Cuts at: 5989 5989
Size: 7601
AleI CACnn'nnGTG
Cuts at: 253 733 889 253
Size: 480 156 6965
Fragments arranged by size:
6965 480 156
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1057 1240 2548 3982 1057
Size: 183 1308 1434 4676
Fragments arranged by size:
4676 1434 1308 183
AlwNI CAG_nnn'CTG
Cuts at: 1311 1320 1415 1701 1977 2380 2484 4138 4255
Size: 9 95 286 276 403 104 1654 117
Cuts at: 4255 4906 6473 1311
Size: 651 1567 2439
Fragments arranged by size:
2439 1654 1567 651 403 286 276 117
104 95 9
ApaI G_GGCC'C
Cuts at: 218 685 1327 1788 2277 2400 4361 218
Size: 467 642 461 489 123 1961 3458
Fragments arranged by size:
3458 1961 642 489 467 461 123
ApaLI G'TGCA_C
Cuts at: 4321 6371 4321
Size: 2050 5551
ApoI r'AATT_y
Cuts at: 2047 3560 4605 6954 7210 2047
Size: 1513 1045 2349 256 2438
Fragments arranged by size:
2438 2349 1513 1045 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7601
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7601
AvaI C'yCGr_G
Cuts at: 520 796 1940 2164 2471 3008 3077 3407 4621
Size: 276 1144 224 307 537 69 330 1214
Cuts at: 4621 520
Size: 3500
Fragments arranged by size:
3500 1214 1144 537 330 307 276 224
69
BamHI G'GATC_C
Cuts at: 3421 3535 4626 3421
Size: 114 1091 6396
Fragments arranged by size:
6396 1091 114
BanI G'GyrC_C
Cuts at: 524 925 1291 2402 2463 3259 3280 3636 3754
Size: 401 366 1111 61 796 21 356 118
Cuts at: 3754 4523 4617 5031 5279 5314 524
Size: 769 94 414 248 35 2811
Fragments arranged by size:
2811 1111 796 769 414 401 366 356
248 118 94 61 35 21
BanII G_rGCy'C
Cuts at: 218 245 685 1327 1788 1896 2144 2277 2400
Size: 27 440 642 461 108 248 133 123
Cuts at: 2400 2885 3169 3442 3842 3913 4340 4361 4615
Size: 485 284 273 400 71 427 21 254
Cuts at: 4615 5645 218
Size: 1030 2174
Fragments arranged by size:
2174 1030 642 485 461 440 427 400
284 273 254 248 133 123 108 71
27 21
BbeI G_GCGC'C
Cuts at: 5035 5283 5035
Size: 248 7353
BbsI GAAGACnn'nnnn_
Cuts at: 804 870 1848 3388 3490 804
Size: 66 978 1540 102 4915
Fragments arranged by size:
4915 1540 978 102 66
BbvCI CC'TCA_GC
Cuts at: 4519 4519
Size: 7601
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2155 2189 3803 3837 2155
Size: 34 1614 34 5919
Fragments arranged by size:
5919 1614 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1278 1540 5499 6260 7601 1278
Size: 262 3959 761 1341 1278
Fragments arranged by size:
3959 1341 1278 761 262
BclI T'GATC_A
Cuts at: 5121 5121
Size: 7601
BfrBI ATG'CAT
Cuts at: 308 308
Size: 7601
BglI GCCn_nnn'nGGC
Cuts at: 129 3013 3040 3164 129
Size: 2884 27 124 4566
Fragments arranged by size:
4566 2884 124 27
BglII A'GATC_T
Cuts at: 5116 7153 5116
Size: 2037 5564
BlpI GC'TnA_GC
Cuts at: 239 1000 3174 3441 4713 239
Size: 761 2174 267 1272 3127
Fragments arranged by size:
3127 2174 1272 761 267
Bme1580I G_kGCm'C
Cuts at: 218 276 293 527 631 685 809 875 990
Size: 58 17 234 104 54 124 66 115
Cuts at: 990 1026 1327 1462 1480 1509 1517 1788 2277
Size: 36 301 135 18 29 8 271 489
Cuts at: 2277 2400 2417 2645 3009 3018 3095 3189 3285
Size: 123 17 228 364 9 77 94 96
Cuts at: 3285 3639 3695 3860 4325 4361 5226 5319 6375
Size: 354 56 165 465 36 865 93 1056
Cuts at: 6375 218
Size: 1444
Fragments arranged by size:
1444 1056 865 489 465 364 354 301
271 234 228 165 135 124 123 115
104 96 94 93 77 66 58 56
54 36 36 29 18 17 17 9
8
BmrI ACTGGGnnnn_n'
Cuts at: 203 286 608 1000 1036 1504 2199 3183 3722
Size: 83 322 392 36 468 695 984 539
Cuts at: 3722 4043 4813 4894 5229 7373 203
Size: 321 770 81 335 2144 431
Fragments arranged by size:
2144 984 770 695 539 468 431 392
335 322 321 83 81 36
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1834 2031 5968 1834
Size: 197 3937 3467
Fragments arranged by size:
3937 3467 197
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 497 1521 1762 1813 1935 2638 3170 3955 4230
Size: 1024 241 51 122 703 532 785 275
Cuts at: 4230 4571 497
Size: 341 3527
Fragments arranged by size:
3527 1024 785 703 532 341 275 241
122 51
Bpu10I CC'TnA_GC
Cuts at: 1328 2096 2387 3121 3168 3344 3907 4519 1328
Size: 768 291 734 47 176 563 612 4410
Fragments arranged by size:
4410 768 734 612 563 291 176 47
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1825 2799 4777 5656 6148 6446 1825
Size: 974 1978 879 492 298 2980
Fragments arranged by size:
2980 1978 974 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1203 2948 7305 1203
Size: 1745 4357 1499
Fragments arranged by size:
4357 1745 1499
BsaAI yAC'GTr
Cuts at: 4443 4954 4443
Size: 511 7090
BsaBI GATnn'nnATC
Cuts at: 6 5115 7152 6
Size: 5109 2037 455
Fragments arranged by size:
5109 2037 455
BsaHI Gr'CG_yC
Cuts at: 2718 2976 5032 5280 5982 7478 2718
Size: 258 2056 248 702 1496 2841
Fragments arranged by size:
2841 2056 1496 702 258 248
BsaWI w'CCGG_w
Cuts at: 2738 4790 5311 5989 6263 6410 6821 2738
Size: 2052 521 678 274 147 411 3518
Fragments arranged by size:
3518 2052 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1111 1141 2830 2860 3922 3952 1111
Size: 30 1689 30 1062 30 4760
Fragments arranged by size:
4760 1689 1062 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 130 623 784 1243 1618 1741 2023 2137 2182
Size: 493 161 459 375 123 282 114 45
Cuts at: 2182 2312 3151 4528 130
Size: 130 839 1377 3203
Fragments arranged by size:
3203 1377 839 493 459 375 282 161
130 123 114 45
BseYI C'CCAG_C
Cuts at: 154 322 420 684 753 2308 2409 2726 2938
Size: 168 98 264 69 1555 101 317 212
Cuts at: 2938 2958 3276 3369 3632 3736 3842 4232 5026
Size: 20 318 93 263 104 106 390 794
Cuts at: 5026 5784 6361 6857 154
Size: 758 577 496 898
Fragments arranged by size:
1555 898 794 758 577 496 390 318
317 264 263 212 168 106 104 101
98 93 69 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 923 1246 1432 1963 2470 2497 3385 4360 923
Size: 323 186 531 507 27 888 975 4164
Fragments arranged by size:
4164 975 888 531 507 323 186 27
BsiEI CG_ry'CG
Cuts at: 104 2461 5143 5189 6397 104
Size: 2357 2682 46 1208 1308
Fragments arranged by size:
2682 2357 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 177 896 1361 1370 1896 2017 2144 2537 3442
Size: 719 465 9 526 121 127 393 905
Cuts at: 3442 3806 4208 4325 4584 4615 5393 5583 6375
Size: 364 402 117 259 31 778 190 792
Cuts at: 6375 177
Size: 1403
Fragments arranged by size:
1403 905 792 778 719 526 465 402
393 364 259 190 127 121 117 31
9
BsmI GAATG_Cn'
Cuts at: 3332 4597 3332
Size: 1265 6336
BsmBI CGTCTCn'nnnn_
Cuts at: 1137 1434 1137
Size: 297 7304
Bsp1286I G_dGCh'C
Cuts at: 177 218 245 276 293 527 631 685 809
Size: 41 27 31 17 234 104 54 124
Cuts at: 809 875 896 990 1026 1327 1361 1370 1462
Size: 66 21 94 36 301 34 9 92
Cuts at: 1462 1480 1509 1517 1788 1896 2017 2144 2277
Size: 18 29 8 271 108 121 127 133
Cuts at: 2277 2400 2417 2537 2645 2885 3009 3018 3095
Size: 123 17 120 108 240 124 9 77
Cuts at: 3095 3169 3189 3285 3442 3639 3695 3806 3842
Size: 74 20 96 157 197 56 111 36
Cuts at: 3842 3860 3913 4208 4325 4340 4361 4584 4615
Size: 18 53 295 117 15 21 223 31
Cuts at: 4615 5226 5319 5393 5583 5645 6375 177
Size: 611 93 74 190 62 730 1403
Fragments arranged by size:
1403 730 611 301 295 271 240 234
223 197 190 157 133 127 124 124
123 121 120 117 111 108 108 104
96 94 93 92 77 74 74 66
62 56 54 53 41 36 36 34
31 31 29 27 21 21 20 18
18 17 17 15 9 9 8
BspEI T'CCGG_A
Cuts at: 2738 6821 2738
Size: 4083 3518
BspHI T'CATG_A
Cuts at: 6777 6777
Size: 7601
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1409 2832 3368 3673 4269 4388 4651 4865 5167
Size: 1423 536 305 596 119 263 214 302
Cuts at: 5167 5548 1409
Size: 381 3462
Fragments arranged by size:
3462 1423 596 536 381 305 302 263
214 119
BsrBI CCG'CTC
Cuts at: 50 120 1014 2522 3144 5893 5947 7431 7596
Size: 70 894 1508 622 2749 54 1484 165
Cuts at: 7596 50
Size: 55
Fragments arranged by size:
2749 1508 1484 894 622 165 70 55
54
BsrDI GCAATG_nn'
Cuts at: 5513 5513
Size: 7601
BsrFI r'CCGG_y
Cuts at: 548 647 1049 3034 4526 5599 5780 5989 548
Size: 99 402 1985 1492 1073 181 209 2160
Fragments arranged by size:
2160 1985 1492 1073 402 209 181 99
BssSI C'ACGA_G
Cuts at: 1895 5872 6230 1895
Size: 3977 358 3266
Fragments arranged by size:
3977 3266 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1311 1374 2484 2595 4978 1311
Size: 63 1110 111 2383 3934
Fragments arranged by size:
3934 2383 1110 111 63
BstBI TT'CG_AA
Cuts at: 5962 5962
Size: 7601
BstXI CCAn_nnnn'nTGG
Cuts at: 732 1451 4115 4503 732
Size: 719 2664 388 3830
Fragments arranged by size:
3830 2664 719 388
BstYI r'GATC_y
Cuts at: 1 3421 3535 4226 4626 5093 5116 5451 5697
Size: 3420 114 691 400 467 23 335 246
Cuts at: 5697 6709 6795 6807 7153 1
Size: 1012 86 12 346 449
Fragments arranged by size:
3420 1012 691 467 449 400 346 335
246 114 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 185 1724 185
Size: 1539 6062
BtgI C'CryG_G
Cuts at: 107 824 1007 3386 107
Size: 717 183 2379 4322
Fragments arranged by size:
4322 2379 717 183
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 249 1044 5551 5732 5895 249
Size: 795 4507 181 163 1955
Fragments arranged by size:
4507 1955 795 181 163
BtsI GCAGTG_nn'
Cuts at: 695 1342 1444 1462 1775 2196 2415 2482 3009
Size: 647 102 18 313 421 219 67 527
Cuts at: 3009 3425 3462 3966 4844 695
Size: 416 37 504 878 3452
Fragments arranged by size:
3452 878 647 527 504 421 416 313
219 102 67 37 18
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3219 3254 3219
Size: 35 7566
DraI TTT'AAA
Cuts at: 4600 4600
Size: 7601
DraIII CAC_nnn'GTG
Cuts at: 2713 2713
Size: 7601
DrdI GACnn_nn'nnGTC
Cuts at: 5307 6165 5307
Size: 858 6743
EaeI y'GGCC_r
Cuts at: 232 1393 1673 1776 2695 4246 4493 5186 5360
Size: 1161 280 103 919 1551 247 693 174
Cuts at: 5360 5751 5778 7123 232
Size: 391 27 1345 710
Fragments arranged by size:
1551 1345 1161 919 710 693 391 280
247 174 103 27
EagI C'GGCC_G
Cuts at: 5186 5186
Size: 7601
EarI CTCTTCn'nnn_
Cuts at: 1609 2261 2921 2980 5624 5834 1609
Size: 652 660 59 2644 210 3376
Fragments arranged by size:
3376 2644 660 652 210 59
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 2758 5809 6119 6265 7411 127
Size: 2631 3051 310 146 1146 317
Fragments arranged by size:
3051 2631 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 1894 2142 3440 4613 1894
Size: 248 1298 1173 4882
Fragments arranged by size:
4882 1298 1173 248
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 497 1521 1762 1813 1935 2638 2902 3170 3955
Size: 1024 241 51 122 703 264 268 785
Cuts at: 3955 4230 4571 5426 5858 6605 6873 497
Size: 275 341 855 432 747 268 1225
Fragments arranged by size:
1225 1024 855 785 747 703 432 341
275 268 268 264 241 122 51
EcoNI CCTnn'n_nnAGG
Cuts at: 718 2285 718
Size: 1567 6034
EcoO109I rG'GnC_Cy
Cuts at: 215 517 681 1138 1324 1422 1784 1878 2273
Size: 302 164 457 186 98 362 94 395
Cuts at: 2273 2274 2290 3066 3201 3226 3465 4104 4252
Size: 1 16 776 135 25 239 639 148
Cuts at: 4252 4357 4358 4740 215
Size: 105 1 382 3076
Fragments arranged by size:
3076 776 639 457 395 382 362 302
239 186 164 148 135 105 98 94
25 16 1 1
EcoRI G'AATT_C
Cuts at: 2047 4605 2047
Size: 2558 5043
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 477 4497 5620 477
Size: 4020 1123 2458
Fragments arranged by size:
4020 2458 1123
FspI TGC'GCA
Cuts at: 1045 5382 1045
Size: 4337 3264
HaeII r_GCGC'y
Cuts at: 1007 2306 2898 2925 3417 3519 5035 5283 6305
Size: 1299 592 27 492 102 1516 248 1022
Cuts at: 6305 7284 1007
Size: 979 1324
Fragments arranged by size:
1516 1324 1299 1022 979 592 492 248
102 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 164 196 1202 1234 1720 1752 2113 2145 2830
Size: 32 1006 32 486 32 361 32 685
Cuts at: 2830 2862 2947 2979 3922 3954 4120 4152 4530
Size: 32 85 32 943 32 166 32 378
Cuts at: 4530 4562 5443 5473 5475 5505 164
Size: 32 881 30 2 30 2260
Fragments arranged by size:
2260 1006 943 881 685 486 378 361
166 85 32 32 32 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1905 4640 1905
Size: 2735 4866
KasI G'GCGC_C
Cuts at: 5031 5279 5031
Size: 248 7353
KpnI G_GTAC'C
Cuts at: 4621 4621
Size: 7601
MluI A'CGCG_T
Cuts at: 7034 7034
Size: 7601
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2663 3315 4056 4265 4287 6272 6456 2663
Size: 652 741 209 22 1985 184 3808
Fragments arranged by size:
3808 1985 741 652 209 184 22
MscI TGG'CCA
Cuts at: 1675 4248 4495 5362 1675
Size: 2573 247 867 3914
Fragments arranged by size:
3914 2573 867 247
MslI CAynn'nnrTG
Cuts at: 253 733 889 1063 1222 1987 2420 5590 5717
Size: 480 156 174 159 765 433 3170 127
Cuts at: 5717 253
Size: 2137
Fragments arranged by size:
3170 2137 765 480 433 174 159 156
127
MspA1I CmG'CkG
Cuts at: 238 792 907 1076 1308 1482 1701 1974 2358
Size: 554 115 169 232 174 219 273 384
Cuts at: 2358 2380 3297 3575 4236 4712 5026 5386 6399
Size: 22 917 278 661 476 314 360 1013
Cuts at: 6399 6644 238
Size: 245 1195
Fragments arranged by size:
1195 1013 917 661 554 476 384 360
314 278 273 245 232 219 174 169
115 22
NaeI GCC'GGC
Cuts at: 649 1051 5782 649
Size: 402 4731 2468
Fragments arranged by size:
4731 2468 402
NarI GG'CG_CC
Cuts at: 5032 5280 5032
Size: 248 7353
NcoI C'CATG_G
Cuts at: 107 824 3386 107
Size: 717 2562 4322
Fragments arranged by size:
4322 2562 717
NgoMIV G'CCGG_C
Cuts at: 647 1049 5780 647
Size: 402 4731 2468
Fragments arranged by size:
4731 2468 402
NsiI A_TGCA'T
Cuts at: 310 310
Size: 7601
NspI r_CATG'y
Cuts at: 308 580 678 4654 5154 6061 308
Size: 272 98 3976 500 907 1848
Fragments arranged by size:
3976 1848 907 500 272 98
PasI CC'CwG_GG
Cuts at: 294 582 3160 294
Size: 288 2578 4735
Fragments arranged by size:
4735 2578 288
PciI A'CATG_T
Cuts at: 6057 6057
Size: 7601
PflMI CCAn_nnn'nTGG
Cuts at: 336 1701 4239 4369 336
Size: 1365 2538 130 3568
Fragments arranged by size:
3568 2538 1365 130
PfoI T'CCnGG_A
Cuts at: 3068 3068
Size: 7601
PmeI GTTT'AAAC
Cuts at: 4600 4600
Size: 7601
PmlI CAC'GTG
Cuts at: 4443 4443
Size: 7601
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6740 6772 6740
Size: 32 7569
PpuMI rG'GwC_Cy
Cuts at: 3066 3226 3465 3066
Size: 160 239 7202
Fragments arranged by size:
7202 239 160
PspOMI G'GGCC_C
Cuts at: 214 681 1323 1784 2273 2396 4357 214
Size: 467 642 461 489 123 1961 3458
Fragments arranged by size:
3458 1961 642 489 467 461 123
PstI C_TGCA'G
Cuts at: 938 1307 1316 1925 4385 4648 5333 938
Size: 369 9 609 2460 263 685 3206
Fragments arranged by size:
3206 2460 685 609 369 263 9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7601
PvuII CAG'CTG
Cuts at: 238 907 1308 1701 1974 2380 3297 3575 5026
Size: 669 401 393 273 406 917 278 1451
Cuts at: 5026 5386 238
Size: 360 2453
Fragments arranged by size:
2453 1451 917 669 406 401 393 360
278 273
RsrII CG'GwC_CG
Cuts at: 5796 5796
Size: 7601
SacI G_AGCT'C
Cuts at: 1896 2144 3442 4615 1896
Size: 248 1298 1173 4882
Fragments arranged by size:
4882 1298 1173 248
SalI G'TCGA_C
Cuts at: 4638 4638
Size: 7601
SanDI GG'GwC_CC
Cuts at: 3226 3226
Size: 7601
SapI GCTCTTCn'nnn_
Cuts at: 1609 5624 5834 1609
Size: 4015 210 3376
Fragments arranged by size:
4015 3376 210
SbfI CC_TGCA'GG
Cuts at: 1925 4648 1925
Size: 2723 4878
ScaI AGT'ACT
Cuts at: 2209 7118 2209
Size: 4909 2692
SexAI A'CCwGG_T
Cuts at: 2709 2709
Size: 7601
SfcI C'TryA_G
Cuts at: 33 486 934 1303 1312 1921 4381 4644 5329
Size: 453 448 369 9 609 2460 263 685
Cuts at: 5329 6322 6513 33
Size: 993 191 1121
Fragments arranged by size:
2460 1121 993 685 609 453 448 369
263 191 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 3040 3040
Size: 7601
SfoI GGC'GCC
Cuts at: 5033 5281 5033
Size: 248 7353
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7601
SgrAI Cr'CCGG_yG
Cuts at: 4526 4526
Size: 7601
SmaI CCC'GGG
Cuts at: 522 1942 3079 3409 4623 522
Size: 1420 1137 330 1214 3500
Fragments arranged by size:
3500 1420 1214 1137 330
SmlI C'TyrA_G
Cuts at: 1840 2778 4756 5671 6163 6425 1840
Size: 938 1978 915 492 262 3016
Fragments arranged by size:
3016 1978 938 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7601
SphI G_CATG'C
Cuts at: 580 4654 5154 580
Size: 4074 500 3027
Fragments arranged by size:
4074 3027 500
StuI AGG'CCT
Cuts at: 3058 4064 3058
Size: 1006 6595
StyI C'CwwG_G
Cuts at: 107 335 824 955 1154 1806 3059 3230 3386
Size: 228 489 131 199 652 1253 171 156
Cuts at: 3386 4114 4550 4735 5089 107
Size: 728 436 185 354 2619
Fragments arranged by size:
2619 1253 728 652 489 436 354 228
199 185 171 156 131
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 192 349 1888 2093 3268 3827 4380 5184 5317
Size: 157 1539 205 1175 559 553 804 133
Cuts at: 5317 5776 5985 192
Size: 459 209 1808
Fragments arranged by size:
1808 1539 1175 804 559 553 459 209
205 157 133
TatI w'GTAC_w
Cuts at: 2207 4164 4469 7116 2207
Size: 1957 305 2647 2692
Fragments arranged by size:
2692 2647 1957 305
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2677 2709 2966 2998 3074 3106 7118 7150 2677
Size: 32 257 32 76 32 4012 32 3128
Fragments arranged by size:
4012 3128 257 76 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5398 5398
Size: 7601
XbaI T'CTAG_A
Cuts at: 4632 4632
Size: 7601
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 252 319 1714 3201 5096 252
Size: 67 1395 1487 1895 2757
Fragments arranged by size:
2757 1895 1487 1395 67
XmaI C'CCGG_G
Cuts at: 520 1940 3077 3407 4621 520
Size: 1420 1137 330 1214 3500
Fragments arranged by size:
3500 1420 1214 1137 330
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BmgBI BmtI BsiWI BsrGI BssHII BstEII BstZ17I ClaI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI PacI PshAI PsiI PspXI PsrI SacII
SnaBI SrfI SspI SwaI XhoI XmnI ZraI