Restriction map for
pF1KA0491
(Circular) (Six-base) MAPSORT of: pF1KA0491.seq Check: 7726 from: 1 to: 4208
pF1KA0491 4208 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 1246 1246
Size: 4208
Acc65I G'GTAC_C
Cuts at: 1224 1224
Size: 4208
AclI AA'CG_TT
Cuts at: 4048 4048
Size: 4208
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 2033 2465 3212 3480 317
Size: 1716 432 747 268 1045
Fragments arranged by size:
1716 1045 747 432 268
AflII C'TTAA_G
Cuts at: 1009 1009
Size: 4208
AflIII A'CryG_T
Cuts at: 1560 2664 3641 1560
Size: 1104 977 2127
Fragments arranged by size:
2127 1104 977
AgeI A'CCGG_T
Cuts at: 2596 2596
Size: 4208
AlwNI CAG_nnn'CTG
Cuts at: 424 1513 3080 424
Size: 1089 1567 1552
Fragments arranged by size:
1567 1552 1089
ApaLI G'TGCA_C
Cuts at: 2978 2978
Size: 4208
ApoI r'AATT_y
Cuts at: 337 531 672 709 1212 3561 3817 337
Size: 194 141 37 503 2349 256 728
Fragments arranged by size:
2349 728 503 256 194 141 37
AseI AT'TA_AT
Cuts at: 20 20
Size: 4208
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4208
AvaI C'yCGr_G
Cuts at: 1228 1228
Size: 4208
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 902 935 902
Size: 33 4175
BamHI G'GATC_C
Cuts at: 1233 1233
Size: 4208
BanI G'GyrC_C
Cuts at: 154 1173 1224 1638 1886 1921 154
Size: 1019 51 414 248 35 2441
Fragments arranged by size:
2441 1019 414 248 51 35
BanII G_rGCy'C
Cuts at: 1222 2252 1222
Size: 1030 3178
BbeI G_GCGC'C
Cuts at: 1642 1890 1642
Size: 248 3960
BbsI GAAGACnn'nnnn_
Cuts at: 217 217
Size: 4208
BciVI GTATCCnnnnn_n'
Cuts at: 2106 2867 4208 2106
Size: 761 1341 2106
Fragments arranged by size:
2106 1341 761
BclI T'GATC_A
Cuts at: 1100 1728 1100
Size: 628 3580
BglII A'GATC_T
Cuts at: 1723 3760 1723
Size: 2037 2171
BlpI GC'TnA_GC
Cuts at: 1320 1320
Size: 4208
Bme1580I G_kGCm'C
Cuts at: 1833 1926 2982 1833
Size: 93 1056 3059
Fragments arranged by size:
3059 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 871 1420 1501 1836 3980 871
Size: 549 81 335 2144 1099
Fragments arranged by size:
2144 1099 549 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2575 2575
Size: 4208
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 835 835
Size: 4208
Bpu10I CC'TnA_GC
Cuts at: 244 244
Size: 4208
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1384 2263 2755 3053 1384
Size: 879 492 298 2539
Fragments arranged by size:
2539 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3912 3912
Size: 4208
BsaAI yAC'GTr
Cuts at: 1561 1561
Size: 4208
BsaBI GATnn'nnATC
Cuts at: 6 1722 3759 6
Size: 1716 2037 455
Fragments arranged by size:
2037 1716 455
BsaHI Gr'CG_yC
Cuts at: 1639 1887 2589 4085 1639
Size: 248 702 1496 1762
Fragments arranged by size:
1762 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1397 1918 2596 2870 3017 3428 1397
Size: 521 678 274 147 411 2177
Fragments arranged by size:
2177 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1633 2391 2968 3464 1633
Size: 758 577 496 2377
Fragments arranged by size:
2377 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 196
Size: 4208
BsiEI CG_ry'CG
Cuts at: 104 146 1750 1796 3004 104
Size: 42 1604 46 1208 1308
Fragments arranged by size:
1604 1308 1208 46 42
BsiHKAI G_wGCw'C
Cuts at: 1222 2000 2190 2982 1222
Size: 778 190 792 2448
Fragments arranged by size:
2448 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1222 1833 1926 2000 2190 2252 2982 1222
Size: 611 93 74 190 62 730 2448
Fragments arranged by size:
2448 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3428 3428
Size: 4208
BspHI T'CATG_A
Cuts at: 3384 3384
Size: 4208
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1258 1472 1774 2155 1258
Size: 214 302 381 3311
Fragments arranged by size:
3311 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2500 2554 4038 4203 50
Size: 2450 54 1484 165 55
Fragments arranged by size:
2450 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2120 2120
Size: 4208
BsrFI r'CCGG_y
Cuts at: 2206 2387 2596 2206
Size: 181 209 3818
Fragments arranged by size:
3818 209 181
BssSI C'ACGA_G
Cuts at: 2479 2837 2479
Size: 358 3850
BstAPI GCAn_nnn'nTGC
Cuts at: 658 1585 658
Size: 927 3281
BstBI TT'CG_AA
Cuts at: 2569 2569
Size: 4208
BstYI r'GATC_y
Cuts at: 1 1233 1700 1723 2058 2304 3316 3402 3414
Size: 1232 467 23 335 246 1012 86 12
Cuts at: 3414 3760 1
Size: 346 449
Fragments arranged by size:
1232 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 956 956
Size: 4208
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2158 2339 2502 2158
Size: 181 163 3864
Fragments arranged by size:
3864 181 163
BtsI GCAGTG_nn'
Cuts at: 1451 1451
Size: 4208
DraI TTT'AAA
Cuts at: 1207 1207
Size: 4208
DrdI GACnn_nn'nnGTC
Cuts at: 1914 2772 1914
Size: 858 3350
EaeI y'GGCC_r
Cuts at: 143 198 1793 1967 2358 2385 3730 143
Size: 55 1595 174 391 27 1345 621
Fragments arranged by size:
1595 1345 621 391 174 55 27
EagI C'GGCC_G
Cuts at: 143 1793 143
Size: 1650 2558
EarI CTCTTCn'nnn_
Cuts at: 2231 2441 2231
Size: 210 3998
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2416 2726 2872 4018 2416
Size: 310 146 1146 2606
Fragments arranged by size:
2606 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1220 1220
Size: 4208
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 835 2033 2465 3212 3480 317
Size: 518 1198 432 747 268 1045
Fragments arranged by size:
1198 1045 747 518 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1347 1347
Size: 4208
EcoRI G'AATT_C
Cuts at: 1212 1212
Size: 4208
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 211 2227 211
Size: 2016 2192
FspI TGC'GCA
Cuts at: 1989 1989
Size: 4208
HaeII r_GCGC'y
Cuts at: 1642 1890 2912 3891 1642
Size: 248 1022 979 1959
Fragments arranged by size:
1959 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2050 2080 2082 2112 2050
Size: 30 2 30 4146
Fragments arranged by size:
4146 30 30 2
HincII GTy'rAC
Cuts at: 1247 1247
Size: 4208
HindIII A'AGCT_T
Cuts at: 193 193
Size: 4208
KasI G'GCGC_C
Cuts at: 1638 1886 1638
Size: 248 3960
KpnI G_GTAC'C
Cuts at: 1228 1228
Size: 4208
MfeI C'AATT_G
Cuts at: 578 578
Size: 4208
MluI A'CGCG_T
Cuts at: 3641 3641
Size: 4208
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 824 1019 1101 2879 3063 824
Size: 195 82 1778 184 1969
Fragments arranged by size:
1969 1778 195 184 82
MscI TGG'CCA
Cuts at: 200 1969 200
Size: 1769 2439
MslI CAynn'nnrTG
Cuts at: 2197 2324 2197
Size: 127 4081
MspA1I CmG'CkG
Cuts at: 1319 1633 1993 3006 3251 1319
Size: 314 360 1013 245 2276
Fragments arranged by size:
2276 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2389 2389
Size: 4208
NarI GG'CG_CC
Cuts at: 1639 1887 1639
Size: 248 3960
NcoI C'CATG_G
Cuts at: 956 956
Size: 4208
NgoMIV G'CCGG_C
Cuts at: 2387 2387
Size: 4208
NspI r_CATG'y
Cuts at: 769 1261 1761 2668 769
Size: 492 500 907 2309
Fragments arranged by size:
2309 907 500 492
PciI A'CATG_T
Cuts at: 2664 2664
Size: 4208
PflMI CCAn_nnn'nTGG
Cuts at: 269 269
Size: 4208
PmeI GTTT'AAAC
Cuts at: 1207 1207
Size: 4208
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3347 3379 3347
Size: 32 4176
PstI C_TGCA'G
Cuts at: 663 1255 1940 663
Size: 592 685 2931
Fragments arranged by size:
2931 685 592
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4208
PvuII CAG'CTG
Cuts at: 1633 1993 1633
Size: 360 3848
RsrII CG'GwC_CG
Cuts at: 2403 2403
Size: 4208
SacI G_AGCT'C
Cuts at: 1222 1222
Size: 4208
SalI G'TCGA_C
Cuts at: 1245 1245
Size: 4208
SapI GCTCTTCn'nnn_
Cuts at: 2231 2441 2231
Size: 210 3998
SbfI CC_TGCA'GG
Cuts at: 1255 1255
Size: 4208
ScaI AGT'ACT
Cuts at: 1071 3725 1071
Size: 2654 1554
SfcI C'TryA_G
Cuts at: 33 659 1251 1936 2929 3120 33
Size: 626 592 685 993 191 1121
Fragments arranged by size:
1121 993 685 626 592 191
SfoI GGC'GCC
Cuts at: 1640 1888 1640
Size: 248 3960
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4208
SmaI CCC'GGG
Cuts at: 1230 1230
Size: 4208
SmlI C'TyrA_G
Cuts at: 1009 1363 2278 2770 3032 1009
Size: 354 915 492 262 2185
Fragments arranged by size:
2185 915 492 354 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4208
SphI G_CATG'C
Cuts at: 1261 1761 1261
Size: 500 3708
StyI C'CwwG_G
Cuts at: 956 1342 1696 956
Size: 386 354 3468
Fragments arranged by size:
3468 386 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1791 1924 2383 2592 1791
Size: 133 459 209 3407
Fragments arranged by size:
3407 459 209 133
TatI w'GTAC_w
Cuts at: 760 1069 3723 760
Size: 309 2654 1245
Fragments arranged by size:
2654 1245 309
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3725 3757 3725
Size: 32 4176
Tth111I GACn'n_nGTC
Cuts at: 2005 2005
Size: 4208
XbaI T'CTAG_A
Cuts at: 1239 1239
Size: 4208
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 487 1703 114
Size: 373 1216 2619
Fragments arranged by size:
2619 1216 373
XmaI C'CCGG_G
Cuts at: 1228 1228
Size: 4208
XmnI GAAnn'nnTTC
Cuts at: 512 1128 512
Size: 616 3592
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BbvCI BcgI BfrBI BglI BmgBI BmtI
BsaXI BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI