Restriction map for
pF1KA0494
(Circular) (Six-base) MAPSORT of: pF1KA0494.seq Check: 1544 from: 1 to: 4598
pF1KA0494 4598 bp
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AccI GT'mk_AC
Cuts at: 1636 1636
Size: 4598
Acc65I G'GTAC_C
Cuts at: 956 1614 956
Size: 658 3940
AclI AA'CG_TT
Cuts at: 4438 4438
Size: 4598
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 664 836 1071 1408 1436 2423 2855 3208 3870
Size: 172 235 337 28 987 432 353 662
Cuts at: 3870 664
Size: 1392
Fragments arranged by size:
1392 987 662 432 353 337 235 172
28
AflIII A'CryG_T
Cuts at: 1950 3750 4031 1950
Size: 1800 281 2517
Fragments arranged by size:
2517 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 231 231
Size: 4598
AlwNI CAG_nnn'CTG
Cuts at: 229 1903 3341 229
Size: 1674 1438 1486
Fragments arranged by size:
1674 1486 1438
ApaLI G'TGCA_C
Cuts at: 3436 3436
Size: 4598
ApoI r'AATT_y
Cuts at: 256 430 891 1197 1336 1602 3951 4207 256
Size: 174 461 306 139 266 2349 256 647
Fragments arranged by size:
2349 647 461 306 266 256 174 139
AseI AT'TA_AT
Cuts at: 20 20
Size: 4598
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4598
AvaI C'yCGr_G
Cuts at: 982 1618 982
Size: 636 3962
AvrII C'CTAG_G
Cuts at: 1494 1494
Size: 4598
BamHI G'GATC_C
Cuts at: 1623 1623
Size: 4598
BanI G'GyrC_C
Cuts at: 956 1614 2028 2276 2311 956
Size: 658 414 248 35 3243
Fragments arranged by size:
3243 658 414 248 35
BanII G_rGCy'C
Cuts at: 128 1612 2642 128
Size: 1484 1030 2084
Fragments arranged by size:
2084 1484 1030
BbeI G_GCGC'C
Cuts at: 2032 2280 2032
Size: 248 4350
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1030 1064 1030
Size: 34 4564
BciVI GTATCCnnnnn_n'
Cuts at: 1218 2496 3552 4598 1218
Size: 1278 1056 1046 1218
Fragments arranged by size:
1278 1218 1056 1046
BclI T'GATC_A
Cuts at: 1235 2118 1235
Size: 883 3715
BfrBI ATG'CAT
Cuts at: 133 133
Size: 4598
BglII A'GATC_T
Cuts at: 317 1105 1419 2113 4150 317
Size: 788 314 694 2037 765
Fragments arranged by size:
2037 788 765 694 314
BlpI GC'TnA_GC
Cuts at: 1710 1710
Size: 4598
Bme1580I G_kGCm'C
Cuts at: 948 2223 2316 3440 948
Size: 1275 93 1124 2106
Fragments arranged by size:
2106 1275 1124 93
BmgBI CAC'GTC
Cuts at: 1455 1455
Size: 4598
BmrI ACTGGGnnnn_n'
Cuts at: 1354 1810 1891 2226 4370 1354
Size: 456 81 335 2144 1582
Fragments arranged by size:
2144 1582 456 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 255 1243 2965 255
Size: 988 1722 1888
Fragments arranged by size:
1888 1722 988
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 554 1213 1259 1588 554
Size: 659 46 329 3564
Fragments arranged by size:
3564 659 329 46
Bpu10I CC'TnA_GC
Cuts at: 1573 1573
Size: 4598
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 406 538 1545 1774 2653 3367 3665 385
Size: 21 132 1007 229 879 714 298 1318
Fragments arranged by size:
1318 1007 879 714 298 229 132 21
BsaI GGTCTCn'nnnn_
Cuts at: 1273 1349 4302 1273
Size: 76 2953 1569
Fragments arranged by size:
2953 1569 76
BsaAI yAC'GTr
Cuts at: 1951 1951
Size: 4598
BsaBI GATnn'nnATC
Cuts at: 6 565 797 2112 4149 6
Size: 559 232 1315 2037 455
Fragments arranged by size:
2037 1315 559 455 232
BsaHI Gr'CG_yC
Cuts at: 2029 2277 2979 4475 2029
Size: 248 702 1496 2152
Fragments arranged by size:
2152 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1787 2308 2986 3397 3544 3818 1787
Size: 521 678 411 147 274 2567
Fragments arranged by size:
2567 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 198 228 1346 1376 198
Size: 30 1118 30 3420
Fragments arranged by size:
3420 1118 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 946 946
Size: 4598
BseYI C'CCAG_C
Cuts at: 148 2023 2781 3446 3854 148
Size: 1875 758 665 408 892
Fragments arranged by size:
1875 892 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 2140 2186 3416 104
Size: 2036 46 1230 1286
Fragments arranged by size:
2036 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 128 1245 1612 2390 2580 3440 128
Size: 1117 367 778 190 860 1286
Fragments arranged by size:
1286 1117 860 778 367 190
Bsp1286I G_dGCh'C
Cuts at: 128 948 1245 1612 2223 2316 2390 2580 2642
Size: 820 297 367 611 93 74 190 62
Cuts at: 2642 3440 128
Size: 798 1286
Fragments arranged by size:
1286 820 798 611 367 297 190 93
74 62
BspHI T'CATG_A
Cuts at: 3030 3030
Size: 4598
BspMI ACCTGCnnnn'nnnn_
Cuts at: 381 1648 1862 2164 2545 381
Size: 1267 214 302 381 2434
Fragments arranged by size:
2434 1267 381 302 214
BsrBI CCG'CTC
Cuts at: 50 330 2890 2944 4428 4593 50
Size: 280 2560 54 1484 165 55
Fragments arranged by size:
2560 1484 280 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1327 2510 1327
Size: 1183 3415
BsrFI r'CCGG_y
Cuts at: 2596 2777 2596
Size: 181 4417
BssSI C'ACGA_G
Cuts at: 2869 3577 2869
Size: 708 3890
BstAPI GCAn_nnn'nTGC
Cuts at: 1975 1975
Size: 4598
BstBI TT'CG_AA
Cuts at: 435 2959 435
Size: 2524 2074
BstYI r'GATC_y
Cuts at: 1 317 408 1105 1419 1623 2090 2113 2448
Size: 316 91 697 314 204 467 23 335
Cuts at: 2448 2694 3000 3012 3098 4150 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 697 467 449 335 316 314 306
246 204 91 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1055 2548 2729 2892 1055
Size: 1493 181 163 2761
Fragments arranged by size:
2761 1493 181 163
BtsI GCAGTG_nn'
Cuts at: 614 1841 614
Size: 1227 3371
DraI TTT'AAA
Cuts at: 713 1597 713
Size: 884 3714
DrdI GACnn_nn'nnGTC
Cuts at: 2304 3648 2304
Size: 1344 3254
EaeI y'GGCC_r
Cuts at: 1446 2183 2357 2748 2775 4120 1446
Size: 737 174 391 27 1345 1924
Fragments arranged by size:
1924 1345 737 391 174 27
EagI C'GGCC_G
Cuts at: 2183 2183
Size: 4598
EarI CTCTTCn'nnn_
Cuts at: 239 245 679 1286 2621 2831 239
Size: 6 434 607 1335 210 2006
Fragments arranged by size:
2006 1335 607 434 210 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2806 3548 3694 4408 2806
Size: 742 146 714 2996
Fragments arranged by size:
2996 742 714 146
EcoICRI GAG'CTC
Cuts at: 126 1610 126
Size: 1484 3114
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 554 664 836 1071 1213 1259 1408 1436 1588
Size: 110 172 235 142 46 149 28 152
Cuts at: 1588 2423 2855 3208 3870 554
Size: 835 432 353 662 1282
Fragments arranged by size:
1282 835 662 432 353 235 172 152
149 142 110 46 28
EcoO109I rG'GnC_Cy
Cuts at: 1737 1737
Size: 4598
EcoRI G'AATT_C
Cuts at: 256 1602 256
Size: 1346 3252
EcoRV GAT'ATC
Cuts at: 906 906
Size: 4598
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1145 1598 2617 1145
Size: 453 1019 3126
Fragments arranged by size:
3126 1019 453
FspI TGC'GCA
Cuts at: 2379 2379
Size: 4598
HaeII r_GCGC'y
Cuts at: 2032 2280 3510 4281 2032
Size: 248 1230 771 2349
Fragments arranged by size:
2349 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 211 217 243 249 309 341 1227 1259 2440
Size: 6 26 6 60 32 886 32 1181
Cuts at: 2440 2470 2472 2502 211
Size: 30 2 30 2307
Fragments arranged by size:
2307 1181 886 60 32 32 30 30
26 6 6 2
HincII GTy'rAC
Cuts at: 599 1026 1637 599
Size: 427 611 3560
Fragments arranged by size:
3560 611 427
KasI G'GCGC_C
Cuts at: 2028 2276 2028
Size: 248 4350
KpnI G_GTAC'C
Cuts at: 960 1618 960
Size: 658 3940
MluI A'CGCG_T
Cuts at: 4031 4031
Size: 4598
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 249 3357 3541 249
Size: 3108 184 1306
Fragments arranged by size:
3108 1306 184
MscI TGG'CCA
Cuts at: 1448 2359 1448
Size: 911 3687
MslI CAynn'nnrTG
Cuts at: 574 2587 2714 574
Size: 2013 127 2458
Fragments arranged by size:
2458 2013 127
MspA1I CmG'CkG
Cuts at: 640 1233 1709 2023 2383 3167 3412 640
Size: 593 476 314 360 784 245 1826
Fragments arranged by size:
1826 784 593 476 360 314 245
NaeI GCC'GGC
Cuts at: 2779 2779
Size: 4598
NarI GG'CG_CC
Cuts at: 2029 2277 2029
Size: 248 4350
NgoMIV G'CCGG_C
Cuts at: 2777 2777
Size: 4598
NsiI A_TGCA'T
Cuts at: 135 135
Size: 4598
NspI r_CATG'y
Cuts at: 1651 2151 3754 1651
Size: 500 1603 2495
Fragments arranged by size:
2495 1603 500
PciI A'CATG_T
Cuts at: 3750 3750
Size: 4598
PflMI CCAn_nnn'nTGG
Cuts at: 1455 1455
Size: 4598
PfoI T'CCnGG_A
Cuts at: 822 1565 822
Size: 743 3855
PmeI GTTT'AAAC
Cuts at: 1597 1597
Size: 4598
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3044 3076 3044
Size: 32 4566
PspXI vC'TCGA_Gb
Cuts at: 982 982
Size: 4598
PstI C_TGCA'G
Cuts at: 609 645 788 1645 2330 609
Size: 36 143 857 685 2877
Fragments arranged by size:
2877 857 685 143 36
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4598
PvuII CAG'CTG
Cuts at: 640 1233 2023 2383 640
Size: 593 790 360 2855
Fragments arranged by size:
2855 790 593 360
RsrII CG'GwC_CG
Cuts at: 2793 2793
Size: 4598
SacI G_AGCT'C
Cuts at: 128 1612 128
Size: 1484 3114
SalI G'TCGA_C
Cuts at: 1635 1635
Size: 4598
SapI GCTCTTCn'nnn_
Cuts at: 1286 2621 2831 1286
Size: 1335 210 3053
Fragments arranged by size:
3053 1335 210
SbfI CC_TGCA'GG
Cuts at: 1645 1645
Size: 4598
ScaI AGT'ACT
Cuts at: 4115 4115
Size: 4598
SfcI C'TryA_G
Cuts at: 33 605 641 668 742 784 1641 2326 3294
Size: 572 36 27 74 42 857 685 968
Cuts at: 3294 3485 33
Size: 191 1146
Fragments arranged by size:
1146 968 857 685 572 191 74 42
36 27
SfoI GGC'GCC
Cuts at: 2030 2278 2030
Size: 248 4350
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4598
SmaI CCC'GGG
Cuts at: 1620 1620
Size: 4598
SmlI C'TyrA_G
Cuts at: 400 421 517 982 1524 1753 2668 3382 3644
Size: 21 96 465 542 229 915 714 262
Cuts at: 3644 400
Size: 1354
Fragments arranged by size:
1354 915 714 542 465 262 229 96
21
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4598
SphI G_CATG'C
Cuts at: 1651 2151 1651
Size: 500 4098
StuI AGG'CCT
Cuts at: 1272 1272
Size: 4598
StyI C'CwwG_G
Cuts at: 290 1494 1732 2086 290
Size: 1204 238 354 2802
Fragments arranged by size:
2802 1204 354 238
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1087 2181 2314 2773 2982 1087
Size: 1094 133 459 209 2703
Fragments arranged by size:
2703 1094 459 209 133
TatI w'GTAC_w
Cuts at: 4113 4113
Size: 4598
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 198 230 4115 4147 198
Size: 32 3885 32 649
Fragments arranged by size:
3885 649 32 32
Tth111I GACn'n_nGTC
Cuts at: 2395 2395
Size: 4598
XbaI T'CTAG_A
Cuts at: 1629 1629
Size: 4598
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1456 2093 1456
Size: 637 3961
XhoI C'TCGA_G
Cuts at: 982 982
Size: 4598
XmaI C'CCGG_G
Cuts at: 1618 1618
Size: 4598
XmnI GAAnn'nnTTC
Cuts at: 431 1421 431
Size: 990 3608
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI
PciI PflMI PfoI PmeI PpiI PspXI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AleI AlfI AloI
ApaI AscI BaeI BbsI BbvCI BglI BmtI BsgI
BsiWI BsmI BsmBI BspEI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI PacI PasI PmlI PpuMI PshAI PsiI PspOMI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI ZraI