Restriction map for
pF1KA0512
(Circular) (Six-base) MAPSORT of: pF1KA0512.seq Check: 6994 from: 1 to: 5009
pF1KA0512 5009 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1628 1628
Size: 5009
AccI GT'mk_AC
Cuts at: 179 1019 2047 179
Size: 840 1028 3141
Fragments arranged by size:
3141 1028 840
Acc65I G'GTAC_C
Cuts at: 939 2025 939
Size: 1086 3923
AclI AA'CG_TT
Cuts at: 4849 4849
Size: 5009
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 359 746 1276 1486 2834 3266 4013 4281 359
Size: 387 530 210 1348 432 747 268 1087
Fragments arranged by size:
1348 1087 747 530 432 387 268 210
AfeI AGC'GCT
Cuts at: 420 420
Size: 5009
AflIII A'CryG_T
Cuts at: 2361 3465 4442 2361
Size: 1104 977 2928
Fragments arranged by size:
2928 1104 977
AgeI A'CCGG_T
Cuts at: 3397 3397
Size: 5009
AhdI GACnn_n'nnGTC
Cuts at: 303 692 1152 303
Size: 389 460 4160
Fragments arranged by size:
4160 460 389
AleI CACnn'nnGTG
Cuts at: 682 682
Size: 5009
AlwNI CAG_nnn'CTG
Cuts at: 931 1066 1069 1078 1168 2314 3881 931
Size: 135 3 9 90 1146 1567 2059
Fragments arranged by size:
2059 1567 1146 135 90 9 3
ApaI G_GGCC'C
Cuts at: 357 357
Size: 5009
ApaLI G'TGCA_C
Cuts at: 3779 3779
Size: 5009
ApoI r'AATT_y
Cuts at: 1579 1707 1876 1999 2013 4362 4618 1579
Size: 128 169 123 14 2349 256 1970
Fragments arranged by size:
2349 1970 256 169 128 123 14
AseI AT'TA_AT
Cuts at: 20 1811 20
Size: 1791 3218
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5009
AvaI C'yCGr_G
Cuts at: 535 733 1174 2029 535
Size: 198 441 855 3515
Fragments arranged by size:
3515 855 441 198
BamHI G'GATC_C
Cuts at: 2034 2034
Size: 5009
BanI G'GyrC_C
Cuts at: 163 636 654 939 2025 2439 2687 2722 163
Size: 473 18 285 1086 414 248 35 2450
Fragments arranged by size:
2450 1086 473 414 285 248 35 18
BanII G_rGCy'C
Cuts at: 357 588 897 1175 2023 3053 357
Size: 231 309 278 848 1030 2313
Fragments arranged by size:
2313 1030 848 309 278 231
BbeI G_GCGC'C
Cuts at: 2443 2691 2443
Size: 248 4761
BbsI GAAGACnn'nnnn_
Cuts at: 1209 1209
Size: 5009
BbvCI CC'TCA_GC
Cuts at: 392 422 761 392
Size: 30 339 4640
Fragments arranged by size:
4640 339 30
BciVI GTATCCnnnnn_n'
Cuts at: 2907 3668 5009 2907
Size: 761 1341 2907
Fragments arranged by size:
2907 1341 761
BclI T'GATC_A
Cuts at: 1415 2529 1415
Size: 1114 3895
BglI GCCn_nnn'nGGC
Cuts at: 457 556 1456 457
Size: 99 900 4010
Fragments arranged by size:
4010 900 99
BglII A'GATC_T
Cuts at: 2524 4561 2524
Size: 2037 2972
BlpI GC'TnA_GC
Cuts at: 2121 2121
Size: 5009
Bme1580I G_kGCm'C
Cuts at: 357 776 2634 2727 3783 357
Size: 419 1858 93 1056 1583
Fragments arranged by size:
1858 1583 1056 419 93
BmrI ACTGGGnnnn_n'
Cuts at: 322 797 815 911 1036 2221 2302 2637 4781
Size: 475 18 96 125 1185 81 335 2144
Cuts at: 4781 322
Size: 550
Fragments arranged by size:
2144 1185 550 475 335 125 96 81
18
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3376 3376
Size: 5009
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 275 953 1067 1937 275
Size: 678 114 870 3347
Fragments arranged by size:
3347 870 678 114
Bpu10I CC'TnA_GC
Cuts at: 392 422 761 1944 392
Size: 30 339 1183 3457
Fragments arranged by size:
3457 1183 339 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2185 3064 3556 3854 2185
Size: 879 492 298 3340
Fragments arranged by size:
3340 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 194 1171 4713 194
Size: 977 3542 490
Fragments arranged by size:
3542 977 490
BsaAI yAC'GTr
Cuts at: 1791 2362 1791
Size: 571 4438
BsaBI GATnn'nnATC
Cuts at: 6 2523 4560 6
Size: 2517 2037 455
Fragments arranged by size:
2517 2037 455
BsaHI Gr'CG_yC
Cuts at: 2440 2688 3390 4886 2440
Size: 248 702 1496 2563
Fragments arranged by size:
2563 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2198 2719 3397 3671 3818 4229 2198
Size: 521 678 274 147 411 2978
Fragments arranged by size:
2978 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 458 488 1123 1153 1910 1940 458
Size: 30 635 30 757 30 3527
Fragments arranged by size:
3527 757 635 30 30 30
BseYI C'CCAG_C
Cuts at: 247 271 405 1181 2434 3192 3769 4265 247
Size: 24 134 776 1253 758 577 496 991
Fragments arranged by size:
1253 991 776 758 577 496 134 24
BsiEI CG_ry'CG
Cuts at: 104 2551 2597 3805 104
Size: 2447 46 1208 1308
Fragments arranged by size:
2447 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2023 2801 2991 3783 2023
Size: 778 190 792 3249
Fragments arranged by size:
3249 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 357 588 776 897 1175 2023 2634 2727 2801
Size: 231 188 121 278 848 611 93 74
Cuts at: 2801 2991 3053 3783 357
Size: 190 62 730 1583
Fragments arranged by size:
1583 848 730 611 278 231 190 188
121 93 74 62
BspEI T'CCGG_A
Cuts at: 4229 4229
Size: 5009
BspHI T'CATG_A
Cuts at: 4185 4185
Size: 5009
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1628 2059 2273 2575 2956 1628
Size: 431 214 302 381 3681
Fragments arranged by size:
3681 431 381 302 214
BsrBI CCG'CTC
Cuts at: 50 937 3301 3355 4839 5004 50
Size: 887 2364 54 1484 165 55
Fragments arranged by size:
2364 1484 887 165 55 54
BsrDI GCAATG_nn'
Cuts at: 515 581 1634 2921 515
Size: 66 1053 1287 2603
Fragments arranged by size:
2603 1287 1053 66
BsrFI r'CCGG_y
Cuts at: 1496 3007 3188 3397 1496
Size: 1511 181 209 3108
Fragments arranged by size:
3108 1511 209 181
BsrGI T'GTAC_A
Cuts at: 1508 1508
Size: 5009
BssSI C'ACGA_G
Cuts at: 3280 3638 3280
Size: 358 4651
BstAPI GCAn_nnn'nTGC
Cuts at: 1066 1069 1078 2386 1066
Size: 3 9 1308 3689
Fragments arranged by size:
3689 1308 9 3
BstBI TT'CG_AA
Cuts at: 1705 3370 1705
Size: 1665 3344
BstEII G'GTnAC_C
Cuts at: 684 684
Size: 5009
BstYI r'GATC_y
Cuts at: 1 2034 2501 2524 2859 3105 4117 4203 4215
Size: 2033 467 23 335 246 1012 86 12
Cuts at: 4215 4561 1
Size: 346 449
Fragments arranged by size:
2033 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 482 482
Size: 5009
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 728 2959 3140 3303 728
Size: 2231 181 163 2434
Fragments arranged by size:
2434 2231 181 163
BtsI GCAGTG_nn'
Cuts at: 404 2252 404
Size: 1848 3161
DraI TTT'AAA
Cuts at: 2008 2008
Size: 5009
DrdI GACnn_nn'nnGTC
Cuts at: 2715 3573 2715
Size: 858 4151
EaeI y'GGCC_r
Cuts at: 221 1457 2594 2768 3159 3186 4531 221
Size: 1236 1137 174 391 27 1345 699
Fragments arranged by size:
1345 1236 1137 699 391 174 27
EagI C'GGCC_G
Cuts at: 2594 2594
Size: 5009
EarI CTCTTCn'nnn_
Cuts at: 208 3032 3242 208
Size: 2824 210 1975
Fragments arranged by size:
2824 1975 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1103 3217 3527 3673 4819 1103
Size: 2114 310 146 1146 1293
Fragments arranged by size:
2114 1293 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2021 2021
Size: 5009
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 275 359 746 953 1067 1276 1486 1937 2834
Size: 84 387 207 114 209 210 451 897
Cuts at: 2834 3266 4013 4281 275
Size: 432 747 268 1003
Fragments arranged by size:
1003 897 747 451 432 387 268 210
209 207 114 84
EcoNI CCTnn'n_nnAGG
Cuts at: 1105 1105
Size: 5009
EcoO109I rG'GnC_Cy
Cuts at: 353 549 694 971 1110 1278 2148 353
Size: 196 145 277 139 168 870 3214
Fragments arranged by size:
3214 870 277 196 168 145 139
EcoRI G'AATT_C
Cuts at: 1876 2013 1876
Size: 137 4872
EcoRV GAT'ATC
Cuts at: 1533 1533
Size: 5009
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1245 1909 3028 1245
Size: 664 1119 3226
Fragments arranged by size:
3226 1119 664
FspI TGC'GCA
Cuts at: 2790 2790
Size: 5009
HaeII r_GCGC'y
Cuts at: 422 1227 2443 2691 3713 4692 422
Size: 805 1216 248 1022 979 739
Fragments arranged by size:
1216 1022 979 805 739 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 278 289 310 321 352 384 678 710 1138
Size: 11 21 11 31 32 294 32 428
Cuts at: 1138 1150 1170 1182 2851 2881 2883 2913 278
Size: 12 20 12 1669 30 2 30 2374
Fragments arranged by size:
2374 1669 428 294 32 32 31 30
30 21 20 12 12 11 11 2
HincII GTy'rAC
Cuts at: 2048 2048
Size: 5009
KasI G'GCGC_C
Cuts at: 2439 2687 2439
Size: 248 4761
KpnI G_GTAC'C
Cuts at: 943 2029 943
Size: 1086 3923
MluI A'CGCG_T
Cuts at: 4442 4442
Size: 5009
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 366 1341 1551 3680 3864 366
Size: 975 210 2129 184 1511
Fragments arranged by size:
2129 1511 975 210 184
MscI TGG'CCA
Cuts at: 223 1459 2770 223
Size: 1236 1311 2462
Fragments arranged by size:
2462 1311 1236
MslI CAynn'nnrTG
Cuts at: 682 2998 3125 682
Size: 2316 127 2566
Fragments arranged by size:
2566 2316 127
MspA1I CmG'CkG
Cuts at: 409 634 760 850 871 1066 2120 2434 2794
Size: 225 126 90 21 195 1054 314 360
Cuts at: 2794 3807 4052 409
Size: 1013 245 1366
Fragments arranged by size:
1366 1054 1013 360 314 245 225 195
126 90 21
NaeI GCC'GGC
Cuts at: 1498 3190 1498
Size: 1692 3317
NarI GG'CG_CC
Cuts at: 2440 2688 2440
Size: 248 4761
NgoMIV G'CCGG_C
Cuts at: 1496 3188 1496
Size: 1692 3317
NspI r_CATG'y
Cuts at: 2062 2562 3469 2062
Size: 500 907 3602
Fragments arranged by size:
3602 907 500
PasI CC'CwG_GG
Cuts at: 889 889
Size: 5009
PciI A'CATG_T
Cuts at: 3465 3465
Size: 5009
PfoI T'CCnGG_A
Cuts at: 822 858 973 1044 822
Size: 36 115 71 4787
Fragments arranged by size:
4787 115 71 36
PmeI GTTT'AAAC
Cuts at: 2008 2008
Size: 5009
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4148 4180 4148
Size: 32 4977
PpuMI rG'GwC_Cy
Cuts at: 694 971 1110 1278 694
Size: 277 139 168 4425
Fragments arranged by size:
4425 277 168 139
PsiI TTA'TAA
Cuts at: 1984 1984
Size: 5009
PspOMI G'GGCC_C
Cuts at: 353 353
Size: 5009
PstI C_TGCA'G
Cuts at: 564 798 816 1062 1074 2056 2741 564
Size: 234 18 246 12 982 685 2832
Fragments arranged by size:
2832 982 685 246 234 18 12
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5009
PvuII CAG'CTG
Cuts at: 409 760 871 1066 2434 2794 409
Size: 351 111 195 1368 360 2624
Fragments arranged by size:
2624 1368 360 351 195 111
RsrII CG'GwC_CG
Cuts at: 3204 3204
Size: 5009
SacI G_AGCT'C
Cuts at: 2023 2023
Size: 5009
SalI G'TCGA_C
Cuts at: 2046 2046
Size: 5009
SapI GCTCTTCn'nnn_
Cuts at: 3032 3242 3032
Size: 210 4799
SbfI CC_TGCA'GG
Cuts at: 2056 2056
Size: 5009
ScaI AGT'ACT
Cuts at: 4526 4526
Size: 5009
SexAI A'CCwGG_T
Cuts at: 967 967
Size: 5009
SfcI C'TryA_G
Cuts at: 33 560 794 812 1058 1070 1869 2052 2737
Size: 527 234 18 246 12 799 183 685
Cuts at: 2737 3730 3921 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 799 685 527 246 234 191
183 18 12
SfoI GGC'GCC
Cuts at: 2441 2689 2441
Size: 248 4761
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5009
SmaI CCC'GGG
Cuts at: 537 735 2031 537
Size: 198 1296 3515
Fragments arranged by size:
3515 1296 198
SmlI C'TyrA_G
Cuts at: 2164 3079 3571 3833 2164
Size: 915 492 262 3340
Fragments arranged by size:
3340 915 492 262
SpeI A'CTAG_T
Cuts at: 67 1989 67
Size: 1922 3087
SphI G_CATG'C
Cuts at: 2062 2562 2062
Size: 500 4509
StuI AGG'CCT
Cuts at: 1394 1394
Size: 5009
StyI C'CwwG_G
Cuts at: 300 552 716 2143 2497 300
Size: 252 164 1427 354 2812
Fragments arranged by size:
2812 1427 354 252 164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 690 703 1246 2592 2725 3184 3393 690
Size: 13 543 1346 133 459 209 2306
Fragments arranged by size:
2306 1346 543 459 209 133 13
TatI w'GTAC_w
Cuts at: 1508 4524 1508
Size: 3016 1993
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1123 1155 4526 4558 1123
Size: 32 3371 32 1574
Fragments arranged by size:
3371 1574 32 32
Tth111I GACn'n_nGTC
Cuts at: 2806 2806
Size: 5009
XbaI T'CTAG_A
Cuts at: 2040 2040
Size: 5009
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 216 460 2504 216
Size: 244 2044 2721
Fragments arranged by size:
2721 2044 244
XmaI C'CCGG_G
Cuts at: 535 733 2029 535
Size: 198 1296 3515
Fragments arranged by size:
3515 1296 198
XmnI GAAnn'nnTTC
Cuts at: 1999 1999
Size: 5009
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PfoI PmeI PpiI PpuMI PsiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AflII AlfI AloI AscI AvrII BaeI BcgI
BfrBI BmgBI BmtI BseRI BsgI BsiWI BsmI BsmBI
BssHII BstXI BstZ17I BtgI ClaI CspCI DraIII FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PflMI PmlI PshAI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI ZraI