Restriction map for
pF1KA0519
(Circular) (Six-base) MAPSORT of: pF1KA0519.seq Check: 1907 from: 1 to: 5870
pF1KA0519 5870 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1312 1312
Size: 5870
AccI GT'mk_AC
Cuts at: 695 2908 695
Size: 2213 3657
Acc65I G'GTAC_C
Cuts at: 2886 2886
Size: 5870
AclI AA'CG_TT
Cuts at: 782 5710 782
Size: 4928 942
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 1162 1276 1402 3695 4127 4874 5142 463
Size: 699 114 126 2293 432 747 268 1191
Fragments arranged by size:
2293 1191 747 699 432 268 126 114
AfeI AGC'GCT
Cuts at: 2066 2066
Size: 5870
AflIII A'CryG_T
Cuts at: 3222 4326 5303 3222
Size: 1104 977 3789
Fragments arranged by size:
3789 1104 977
AgeI A'CCGG_T
Cuts at: 4258 4258
Size: 5870
AleI CACnn'nnGTG
Cuts at: 2101 2101
Size: 5870
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2063 2095 2063
Size: 32 5838
AlwNI CAG_nnn'CTG
Cuts at: 489 1290 2008 2512 2703 3175 4742 489
Size: 801 718 504 191 472 1567 1617
Fragments arranged by size:
1617 1567 801 718 504 472 191
ApaLI G'TGCA_C
Cuts at: 4640 4640
Size: 5870
ApoI r'AATT_y
Cuts at: 1297 1990 2053 2196 2874 5223 5479 1297
Size: 693 63 143 678 2349 256 1688
Fragments arranged by size:
2349 1688 693 678 256 143 63
AseI AT'TA_AT
Cuts at: 20 20
Size: 5870
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5870
AvaI C'yCGr_G
Cuts at: 308 629 1414 2084 2399 2890 308
Size: 321 785 670 315 491 3288
Fragments arranged by size:
3288 785 670 491 321 315
BamHI G'GATC_C
Cuts at: 738 2895 738
Size: 2157 3713
BanI G'GyrC_C
Cuts at: 1110 1480 1560 2886 3300 3548 3583 1110
Size: 370 80 1326 414 248 35 3397
Fragments arranged by size:
3397 1326 414 370 248 80 35
BanII G_rGCy'C
Cuts at: 386 542 1085 2002 2884 3914 386
Size: 156 543 917 882 1030 2342
Fragments arranged by size:
2342 1030 917 882 543 156
BbeI G_GCGC'C
Cuts at: 3304 3552 3304
Size: 248 5622
BbsI GAAGACnn'nnnn_
Cuts at: 222 222
Size: 5870
BciVI GTATCCnnnnn_n'
Cuts at: 3768 4529 5870 3768
Size: 761 1341 3768
Fragments arranged by size:
3768 1341 761
BclI T'GATC_A
Cuts at: 3390 3390
Size: 5870
BglI GCCn_nnn'nGGC
Cuts at: 903 1000 903
Size: 97 5773
BglII A'GATC_T
Cuts at: 3385 5422 3385
Size: 2037 3833
BlpI GC'TnA_GC
Cuts at: 202 869 2982 202
Size: 667 2113 3090
Fragments arranged by size:
3090 2113 667
Bme1580I G_kGCm'C
Cuts at: 1347 3495 3588 4644 1347
Size: 2148 93 1056 2573
Fragments arranged by size:
2573 2148 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 999 3082 3163 3498 5642 999
Size: 2083 81 335 2144 1227
Fragments arranged by size:
2144 2083 1227 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1206 2414 4237 1206
Size: 1208 1823 2839
Fragments arranged by size:
2839 1823 1208
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 415 478 895 1913 415
Size: 63 417 1018 4372
Fragments arranged by size:
4372 1018 417 63
Bpu10I CC'TnA_GC
Cuts at: 2001 2001
Size: 5870
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 3046 3925 4417 4715 484
Size: 2562 879 492 298 1639
Fragments arranged by size:
2562 1639 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5574 5574
Size: 5870
BsaAI yAC'GTr
Cuts at: 197 342 822 946 3223 197
Size: 145 480 124 2277 2844
Fragments arranged by size:
2844 2277 480 145 124
BsaBI GATnn'nnATC
Cuts at: 6 3384 5421 6
Size: 3378 2037 455
Fragments arranged by size:
3378 2037 455
BsaHI Gr'CG_yC
Cuts at: 2243 3301 3549 4251 5747 2243
Size: 1058 248 702 1496 2366
Fragments arranged by size:
2366 1496 1058 702 248
BsaWI w'CCGG_w
Cuts at: 1238 3059 3580 4258 4532 4679 5090 1238
Size: 1821 521 678 274 147 411 2018
Fragments arranged by size:
2018 1821 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 868 898 2063 2093 868
Size: 30 1165 30 4645
Fragments arranged by size:
4645 1165 30 30
BseYI C'CCAG_C
Cuts at: 1324 1497 1728 2035 3295 4053 4630 5126 1324
Size: 173 231 307 1260 758 577 496 2068
Fragments arranged by size:
2068 1260 758 577 496 307 231 173
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1285 1285
Size: 5870
BsiEI CG_ry'CG
Cuts at: 104 3412 3458 4666 104
Size: 3308 46 1208 1308
Fragments arranged by size:
3308 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 386 542 1929 2816 2884 3662 3852 4644 386
Size: 156 1387 887 68 778 190 792 1612
Fragments arranged by size:
1612 1387 887 792 778 190 156 68
BsmI GAATG_Cn'
Cuts at: 525 525
Size: 5870
BsmBI CGTCTCn'nnnn_
Cuts at: 1837 1837
Size: 5870
Bsp1286I G_dGCh'C
Cuts at: 386 542 1085 1347 1929 2002 2816 2884 3495
Size: 156 543 262 582 73 814 68 611
Cuts at: 3495 3588 3662 3852 3914 4644 386
Size: 93 74 190 62 730 1612
Fragments arranged by size:
1612 814 730 611 582 543 262 190
156 93 74 73 68 62
BspEI T'CCGG_A
Cuts at: 5090 5090
Size: 5870
BspHI T'CATG_A
Cuts at: 2709 5046 2709
Size: 2337 3533
BspMI ACCTGCnnnn'nnnn_
Cuts at: 169 497 1312 1497 2920 3134 3436 3817 169
Size: 328 815 185 1423 214 302 381 2222
Fragments arranged by size:
2222 1423 815 381 328 302 214 185
BsrBI CCG'CTC
Cuts at: 50 240 1190 1359 1735 2121 2733 4162 4216
Size: 190 950 169 376 386 612 1429 54
Cuts at: 4216 5700 5865 50
Size: 1484 165 55
Fragments arranged by size:
1484 1429 950 612 386 376 190 169
165 55 54
BsrDI GCAATG_nn'
Cuts at: 1244 2082 2606 3782 1244
Size: 838 524 1176 3332
Fragments arranged by size:
3332 1176 838 524
BsrFI r'CCGG_y
Cuts at: 604 638 849 1113 3868 4049 4258 604
Size: 34 211 264 2755 181 209 2216
Fragments arranged by size:
2755 2216 264 211 209 181 34
BsrGI T'GTAC_A
Cuts at: 2786 2786
Size: 5870
BssSI C'ACGA_G
Cuts at: 773 1571 2728 4141 4499 773
Size: 798 1157 1413 358 2144
Fragments arranged by size:
2144 1413 1157 798 358
BstAPI GCAn_nnn'nTGC
Cuts at: 489 3247 489
Size: 2758 3112
BstBI TT'CG_AA
Cuts at: 1196 1463 4231 1196
Size: 267 2768 2835
Fragments arranged by size:
2835 2768 267
BstEII G'GTnAC_C
Cuts at: 2658 2658
Size: 5870
BstXI CCAn_nnnn'nTGG
Cuts at: 1092 1995 2256 1092
Size: 903 261 4706
Fragments arranged by size:
4706 903 261
BstYI r'GATC_y
Cuts at: 1 348 738 1724 1763 2895 3362 3385 3720
Size: 347 390 986 39 1132 467 23 335
Cuts at: 3720 3966 4978 5064 5076 5422 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1132 1012 986 467 449 390 347 346
335 246 86 39 23 12
Bsu36I CC'TnA_GG
Cuts at: 2696 2696
Size: 5870
BtgI C'CryG_G
Cuts at: 995 2495 995
Size: 1500 4370
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 228 792 3820 4001 4164 228
Size: 564 3028 181 163 1934
Fragments arranged by size:
3028 1934 564 181 163
BtsI GCAGTG_nn'
Cuts at: 1543 1670 3113 1543
Size: 127 1443 4300
Fragments arranged by size:
4300 1443 127
DraI TTT'AAA
Cuts at: 2869 2869
Size: 5870
DraIII CAC_nnn'GTG
Cuts at: 1351 1351
Size: 5870
DrdI GACnn_nn'nnGTC
Cuts at: 718 2242 3576 4434 718
Size: 1524 1334 858 2154
Fragments arranged by size:
2154 1524 1334 858
EaeI y'GGCC_r
Cuts at: 987 2424 3455 3629 4020 4047 5392 987
Size: 1437 1031 174 391 27 1345 1465
Fragments arranged by size:
1465 1437 1345 1031 391 174 27
EagI C'GGCC_G
Cuts at: 3455 3455
Size: 5870
EarI CTCTTCn'nnn_
Cuts at: 374 457 1138 1193 1465 1742 2821 3893 4103
Size: 83 681 55 272 277 1079 1072 210
Cuts at: 4103 374
Size: 2141
Fragments arranged by size:
2141 1079 1072 681 277 272 210 83
55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 178 921 2344 4078 4388 4534 5680 178
Size: 743 1423 1734 310 146 1146 368
Fragments arranged by size:
1734 1423 1146 743 368 310 146
EcoICRI GAG'CTC
Cuts at: 384 540 2882 384
Size: 156 2342 3372
Fragments arranged by size:
3372 2342 156
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 415 463 478 895 1162 1276 1402 1913 3695
Size: 48 15 417 267 114 126 511 1782
Cuts at: 3695 4127 4874 5142 415
Size: 432 747 268 1143
Fragments arranged by size:
1782 1143 747 511 432 417 268 267
126 114 48 15
EcoNI CCTnn'n_nnAGG
Cuts at: 1180 2176 1180
Size: 996 4874
EcoO109I rG'GnC_Cy
Cuts at: 486 1287 1419 1821 1933 2220 2700 3009 486
Size: 801 132 402 112 287 480 309 3347
Fragments arranged by size:
3347 801 480 402 309 287 132 112
EcoRI G'AATT_C
Cuts at: 1297 2196 2874 1297
Size: 899 678 4293
Fragments arranged by size:
4293 899 678
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3889 3889
Size: 5870
FspI TGC'GCA
Cuts at: 3651 3651
Size: 5870
HaeII r_GCGC'y
Cuts at: 408 2068 3304 3552 4574 5553 408
Size: 1660 1236 248 1022 979 725
Fragments arranged by size:
1660 1236 1022 979 725 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 288 320 1146 1178 1698 1730 3712 3742 3744
Size: 32 826 32 520 32 1982 30 2
Cuts at: 3744 3774 288
Size: 30 2384
Fragments arranged by size:
2384 1982 826 520 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2111 2909 2111
Size: 798 5072
KasI G'GCGC_C
Cuts at: 3300 3548 3300
Size: 248 5622
KpnI G_GTAC'C
Cuts at: 2890 2890
Size: 5870
MluI A'CGCG_T
Cuts at: 5303 5303
Size: 5870
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 203 603 2498 4541 4725 203
Size: 400 1895 2043 184 1348
Fragments arranged by size:
2043 1895 1348 400 184
MscI TGG'CCA
Cuts at: 3631 3631
Size: 5870
MslI CAynn'nnrTG
Cuts at: 923 2101 2254 2611 2656 2679 3859 3986 923
Size: 1178 153 357 45 23 1180 127 2807
Fragments arranged by size:
2807 1180 1178 357 153 127 45 23
MspA1I CmG'CkG
Cuts at: 393 1062 1759 1916 2981 3295 3655 4668 4913
Size: 669 697 157 1065 314 360 1013 245
Cuts at: 4913 393
Size: 1350
Fragments arranged by size:
1350 1065 1013 697 669 360 314 245
157
NaeI GCC'GGC
Cuts at: 606 640 4051 606
Size: 34 3411 2425
Fragments arranged by size:
3411 2425 34
NarI GG'CG_CC
Cuts at: 3301 3549 3301
Size: 248 5622
NcoI C'CATG_G
Cuts at: 995 2495 995
Size: 1500 4370
NgoMIV G'CCGG_C
Cuts at: 604 638 4049 604
Size: 34 3411 2425
Fragments arranged by size:
3411 2425 34
NspI r_CATG'y
Cuts at: 168 1533 2778 2923 3423 4330 168
Size: 1365 1245 145 500 907 1708
Fragments arranged by size:
1708 1365 1245 907 500 145
PciI A'CATG_T
Cuts at: 4326 4326
Size: 5870
PfoI T'CCnGG_A
Cuts at: 362 1742 2570 362
Size: 1380 828 3662
Fragments arranged by size:
3662 1380 828
PmeI GTTT'AAAC
Cuts at: 2869 2869
Size: 5870
PmlI CAC'GTG
Cuts at: 197 342 197
Size: 145 5725
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2063 2095 5009 5041 2063
Size: 32 2914 32 2892
Fragments arranged by size:
2914 2892 32 32
PpuMI rG'GwC_Cy
Cuts at: 486 1287 1419 1821 2220 486
Size: 801 132 402 399 4136
Fragments arranged by size:
4136 801 402 399 132
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2779 2811 2779
Size: 32 5838
PstI C_TGCA'G
Cuts at: 392 1538 2917 3602 392
Size: 1146 1379 685 2660
Fragments arranged by size:
2660 1379 1146 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5870
PvuII CAG'CTG
Cuts at: 393 1759 1916 3295 3655 393
Size: 1366 157 1379 360 2608
Fragments arranged by size:
2608 1379 1366 360 157
RsrII CG'GwC_CG
Cuts at: 4065 4065
Size: 5870
SacI G_AGCT'C
Cuts at: 386 542 2884 386
Size: 156 2342 3372
Fragments arranged by size:
3372 2342 156
SalI G'TCGA_C
Cuts at: 2907 2907
Size: 5870
SanDI GG'GwC_CC
Cuts at: 1419 1419
Size: 5870
SapI GCTCTTCn'nnn_
Cuts at: 374 457 1465 2821 3893 4103 374
Size: 83 1008 1356 1072 210 2141
Fragments arranged by size:
2141 1356 1072 1008 210 83
SbfI CC_TGCA'GG
Cuts at: 2917 2917
Size: 5870
ScaI AGT'ACT
Cuts at: 984 5387 984
Size: 4403 1467
SfcI C'TryA_G
Cuts at: 33 388 1534 2913 3598 4591 4782 33
Size: 355 1146 1379 685 993 191 1121
Fragments arranged by size:
1379 1146 1121 993 685 355 191
SfoI GGC'GCC
Cuts at: 3302 3550 3302
Size: 248 5622
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5870
SmaI CCC'GGG
Cuts at: 310 1416 2892 310
Size: 1106 1476 3288
Fragments arranged by size:
3288 1476 1106
SmlI C'TyrA_G
Cuts at: 499 3025 3940 4432 4694 499
Size: 2526 915 492 262 1675
Fragments arranged by size:
2526 1675 915 492 262
SpeI A'CTAG_T
Cuts at: 67 828 67
Size: 761 5109
SphI G_CATG'C
Cuts at: 168 2923 3423 168
Size: 2755 500 2615
Fragments arranged by size:
2755 2615 500
StyI C'CwwG_G
Cuts at: 743 995 2495 3004 3358 743
Size: 252 1500 509 354 3255
Fragments arranged by size:
3255 1500 509 354 252
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 535 1433 1881 3453 3586 4045 4254 535
Size: 898 448 1572 133 459 209 2151
Fragments arranged by size:
2151 1572 898 459 448 209 133
TatI w'GTAC_w
Cuts at: 982 1028 2786 5385 982
Size: 46 1758 2599 1467
Fragments arranged by size:
2599 1758 1467 46
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1049 1081 2212 2244 5387 5419 1049
Size: 32 1131 32 3143 32 1500
Fragments arranged by size:
3143 1500 1131 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1048 3667 1048
Size: 2619 3251
XbaI T'CTAG_A
Cuts at: 2901 2901
Size: 5870
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 721 1033 2574 3365 721
Size: 312 1541 791 3226
Fragments arranged by size:
3226 1541 791 312
XmaI C'CCGG_G
Cuts at: 308 1414 2890 308
Size: 1106 1476 3288
Fragments arranged by size:
3288 1476 1106
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PmlI PpiI
PpuMI PsrI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AlfI ApaI AscI AvrII BaeI
BbvCI BcgI BfrBI BmgBI BmtI BseRI BsiWI BssHII
BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PshAI PsiI PspOMI PspXI SacII SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI