Restriction map for
pF1KA0521
(Circular) (Six-base) MAPSORT of: pF1KA0521.seq Check: 3502 from: 1 to: 6158
pF1KA0521 6158 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1959 1959
Size: 6158
AatII G_ACGT'C
Cuts at: 3143 3143
Size: 6158
AccI GT'mk_AC
Cuts at: 2161 3196 2161
Size: 1035 5123
Acc65I G'GTAC_C
Cuts at: 3174 3174
Size: 6158
AclI AA'CG_TT
Cuts at: 5998 5998
Size: 6158
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 499 757 1078 1096 1440 2059 2890 3983 4415
Size: 258 321 18 344 619 831 1093 432
Cuts at: 4415 4768 5430 499
Size: 353 662 1227
Fragments arranged by size:
1227 1093 831 662 619 432 353 344
321 258 18
AfeI AGC'GCT
Cuts at: 2275 2842 2275
Size: 567 5591
AflIII A'CryG_T
Cuts at: 2164 3510 5310 5591 2164
Size: 1346 1800 281 2731
Fragments arranged by size:
2731 1800 1346 281
AhdI GACnn_n'nnGTC
Cuts at: 246 418 1059 246
Size: 172 641 5345
Fragments arranged by size:
5345 641 172
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2138 2138
Size: 6158
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 499 531 2131 2163 499
Size: 32 1600 32 4494
Fragments arranged by size:
4494 1600 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1722 2052 2310 2880 2883 2986 3463 4901 1722
Size: 330 258 570 3 103 477 1438 2979
Fragments arranged by size:
2979 1438 570 477 330 258 103 3
ApaI G_GGCC'C
Cuts at: 498 1454 2602 498
Size: 956 1148 4054
Fragments arranged by size:
4054 1148 956
ApaLI G'TGCA_C
Cuts at: 448 4996 448
Size: 4548 1610
ApoI r'AATT_y
Cuts at: 220 787 3162 5511 5767 220
Size: 567 2375 2349 256 611
Fragments arranged by size:
2375 2349 611 567 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6158
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6158
AvaI C'yCGr_G
Cuts at: 1628 1886 2603 2653 3000 3178 1628
Size: 258 717 50 347 178 4608
Fragments arranged by size:
4608 717 347 258 178 50
BamHI G'GATC_C
Cuts at: 2042 3183 2042
Size: 1141 5017
BanI G'GyrC_C
Cuts at: 1980 3174 3588 3836 3871 1980
Size: 1194 414 248 35 4267
Fragments arranged by size:
4267 1194 414 248 35
BanII G_rGCy'C
Cuts at: 151 498 1088 1378 1454 1954 2602 2836 2854
Size: 347 590 290 76 500 648 234 18
Cuts at: 2854 2944 3007 3172 4202 151
Size: 90 63 165 1030 2107
Fragments arranged by size:
2107 1030 648 590 500 347 290 234
165 90 76 63 18
BbeI G_GCGC'C
Cuts at: 1984 3592 3840 1984
Size: 1608 248 4302
Fragments arranged by size:
4302 1608 248
BbsI GAAGACnn'nnnn_
Cuts at: 950 1055 1109 1156 1619 1769 3143 950
Size: 105 54 47 463 150 1374 3965
Fragments arranged by size:
3965 1374 463 150 105 54 47
BbvCI CC'TCA_GC
Cuts at: 307 307
Size: 6158
BciVI GTATCCnnnnn_n'
Cuts at: 4056 5112 6158 4056
Size: 1056 1046 4056
Fragments arranged by size:
4056 1056 1046
BclI T'GATC_A
Cuts at: 800 1328 3678 800
Size: 528 2350 3280
Fragments arranged by size:
3280 2350 528
BglI GCCn_nnn'nGGC
Cuts at: 155 1402 155
Size: 1247 4911
BglII A'GATC_T
Cuts at: 1577 3673 5710 1577
Size: 2096 2037 2025
Fragments arranged by size:
2096 2037 2025
BlpI GC'TnA_GC
Cuts at: 2725 2853 2938 3270 2725
Size: 128 85 332 5613
Fragments arranged by size:
5613 332 128 85
Bme1580I G_kGCm'C
Cuts at: 452 498 1454 2602 2714 3058 3783 3876 5000
Size: 46 956 1148 112 344 725 93 1124
Cuts at: 5000 452
Size: 1610
Fragments arranged by size:
1610 1148 1124 956 725 344 112 93
46
BmrI ACTGGGnnnn_n'
Cuts at: 883 3370 3451 3786 5930 883
Size: 2487 81 335 2144 1111
Fragments arranged by size:
2487 2144 1111 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2934 3054 4525 2934
Size: 120 1471 4567
Fragments arranged by size:
4567 1471 120
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 375 474 586 640 1150 1606 2041 2203 2257
Size: 99 112 54 510 456 435 162 54
Cuts at: 2257 2332 2395 2416 2449 2500 2614 375
Size: 75 63 21 33 51 114 3919
Fragments arranged by size:
3919 510 456 435 162 114 112 99
75 63 54 54 51 33 21
Bpu10I CC'TnA_GC
Cuts at: 307 307
Size: 6158
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 451 580 1060 1669 2482 3028 3334 4213 4927
Size: 129 480 609 813 546 306 879 714
Cuts at: 4927 5225 451
Size: 298 1384
Fragments arranged by size:
1384 879 813 714 609 546 480 306
298 129
BsaI GGTCTCn'nnnn_
Cuts at: 120 1654 1795 5862 120
Size: 1534 141 4067 416
Fragments arranged by size:
4067 1534 416 141
BsaAI yAC'GTr
Cuts at: 456 3511 456
Size: 3055 3103
BsaBI GATnn'nnATC
Cuts at: 6 3672 5709 6
Size: 3666 2037 455
Fragments arranged by size:
3666 2037 455
BsaHI Gr'CG_yC
Cuts at: 995 1981 3125 3140 3589 3837 4539 6035 995
Size: 986 1144 15 449 248 702 1496 1118
Fragments arranged by size:
1496 1144 1118 986 702 449 248 15
BsaWI w'CCGG_w
Cuts at: 635 3347 3868 4546 4957 5104 5378 635
Size: 2712 521 678 411 147 274 1415
Fragments arranged by size:
2712 1415 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 499 529 1360 1390 1544 1574 2131 2161 3010
Size: 30 831 30 154 30 557 30 849
Cuts at: 3010 3040 499
Size: 30 3617
Fragments arranged by size:
3617 849 831 557 154 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 324 520 637 1324 1456 1459 2236 324
Size: 196 117 687 132 3 777 4246
Fragments arranged by size:
4246 777 687 196 132 117 3
BseYI C'CCAG_C
Cuts at: 134 1622 1737 2057 2276 2447 2552 2572 2843
Size: 1488 115 320 219 171 105 20 271
Cuts at: 2843 3078 3583 4341 5006 5414 134
Size: 235 505 758 665 408 878
Fragments arranged by size:
1488 878 758 665 505 408 320 271
235 219 171 115 105 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 865 1806 2782 865
Size: 941 976 4241
Fragments arranged by size:
4241 976 941
BsiEI CG_ry'CG
Cuts at: 104 2759 3700 3746 4976 104
Size: 2655 941 46 1230 1286
Fragments arranged by size:
2655 1286 1230 941 46
BsiHKAI G_wGCw'C
Cuts at: 452 1378 1954 2854 2917 3172 3950 4140 5000
Size: 926 576 900 63 255 778 190 860
Cuts at: 5000 452
Size: 1610
Fragments arranged by size:
1610 926 900 860 778 576 255 190
63
BsmI GAATG_Cn'
Cuts at: 854 854
Size: 6158
BsmBI CGTCTCn'nnnn_
Cuts at: 562 2104 562
Size: 1542 4616
Bsp1286I G_dGCh'C
Cuts at: 151 452 498 1088 1378 1454 1954 2602 2714
Size: 301 46 590 290 76 500 648 112
Cuts at: 2714 2836 2854 2917 2944 3007 3058 3172 3783
Size: 122 18 63 27 63 51 114 611
Cuts at: 3783 3876 3950 4140 4202 5000 151
Size: 93 74 190 62 798 1309
Fragments arranged by size:
1309 798 648 611 590 500 301 290
190 122 114 112 93 76 74 63
63 62 51 46 27 18
BspHI T'CATG_A
Cuts at: 4590 4590
Size: 6158
BspMI ACCTGCnnnn'nnnn_
Cuts at: 569 1625 1959 2183 3208 3422 3724 4105 569
Size: 1056 334 224 1025 214 302 381 2622
Fragments arranged by size:
2622 1056 1025 381 334 302 224 214
BsrBI CCG'CTC
Cuts at: 50 1524 2136 2193 2370 2385 2433 2469 2933
Size: 1474 612 57 177 15 48 36 464
Cuts at: 2933 4450 4504 5988 6153 50
Size: 1517 54 1484 165 55
Fragments arranged by size:
1517 1484 1474 612 464 177 165 57
55 54 48 36 15
BsrDI GCAATG_nn'
Cuts at: 4070 4070
Size: 6158
BsrFI r'CCGG_y
Cuts at: 2690 3096 4156 4337 2690
Size: 406 1060 181 4511
Fragments arranged by size:
4511 1060 406 181
BssHII G'CGCG_C
Cuts at: 2226 2324 2522 2226
Size: 98 198 5862
Fragments arranged by size:
5862 198 98
BssSI C'ACGA_G
Cuts at: 334 1371 1500 2263 4429 5137 334
Size: 1037 129 763 2166 708 1355
Fragments arranged by size:
2166 1355 1037 763 708 129
BstAPI GCAn_nnn'nTGC
Cuts at: 456 1142 2880 2883 3535 456
Size: 686 1738 3 652 3079
Fragments arranged by size:
3079 1738 686 652 3
BstBI TT'CG_AA
Cuts at: 4519 4519
Size: 6158
BstXI CCAn_nnnn'nTGG
Cuts at: 2019 2784 2019
Size: 765 5393
BstYI r'GATC_y
Cuts at: 1 288 1577 2042 2771 3183 3650 3673 4008
Size: 287 1289 465 729 412 467 23 335
Cuts at: 4008 4254 4560 4572 4658 5710 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1289 1052 729 467 465 449 412 335
306 287 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1030 1030
Size: 6158
BtgI C'CryG_G
Cuts at: 365 1641 2456 365
Size: 1276 815 4067
Fragments arranged by size:
4067 1276 815
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1333 2720 4108 4289 4452 1333
Size: 1387 1388 181 163 3039
Fragments arranged by size:
3039 1388 1387 181 163
BtsI GCAGTG_nn'
Cuts at: 636 3091 3401 636
Size: 2455 310 3393
Fragments arranged by size:
3393 2455 310
DraI TTT'AAA
Cuts at: 218 3157 218
Size: 2939 3219
DraIII CAC_nnn'GTG
Cuts at: 456 456
Size: 6158
DrdI GACnn_nn'nnGTC
Cuts at: 1000 3864 5208 1000
Size: 2864 1344 1950
Fragments arranged by size:
2864 1950 1344
EaeI y'GGCC_r
Cuts at: 330 534 735 1301 1592 1691 1752 2543 2696
Size: 204 201 566 291 99 61 791 153
Cuts at: 2696 2756 3098 3743 3917 4308 4335 5680 330
Size: 60 342 645 174 391 27 1345 808
Fragments arranged by size:
1345 808 791 645 566 391 342 291
204 201 174 153 99 61 60 27
EagI C'GGCC_G
Cuts at: 2756 3743 2756
Size: 987 5171
EarI CTCTTCn'nnn_
Cuts at: 547 1463 4181 4391 547
Size: 916 2718 210 2314
Fragments arranged by size:
2718 2314 916 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1774 4366 5108 5254 5968 1774
Size: 2592 742 146 714 1964
Fragments arranged by size:
2592 1964 742 714 146
EcoICRI GAG'CTC
Cuts at: 1376 1952 2852 3170 1376
Size: 576 900 318 4364
Fragments arranged by size:
4364 900 576 318
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 375 474 499 586 640 757 1078 1096 1150
Size: 99 25 87 54 117 321 18 54
Cuts at: 1150 1440 1606 2041 2059 2203 2257 2332 2395
Size: 290 166 435 18 144 54 75 63
Cuts at: 2395 2416 2449 2500 2614 2890 3983 4415 4768
Size: 21 33 51 114 276 1093 432 353
Cuts at: 4768 5430 375
Size: 662 1103
Fragments arranged by size:
1103 1093 662 435 432 353 321 290
276 166 144 117 114 99 87 75
63 54 54 54 51 33 25 21
18 18
EcoNI CCTnn'n_nnAGG
Cuts at: 306 502 622 952 306
Size: 196 120 330 5512
Fragments arranged by size:
5512 330 196 120
EcoO109I rG'GnC_Cy
Cuts at: 130 494 495 1450 1451 1650 1883 1900 2598
Size: 364 1 955 1 199 233 17 698
Cuts at: 2598 2599 3297 130
Size: 1 698 2991
Fragments arranged by size:
2991 955 698 698 364 233 199 17
1 1 1
EcoRI G'AATT_C
Cuts at: 3162 3162
Size: 6158
EcoRV GAT'ATC
Cuts at: 978 1176 978
Size: 198 5960
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 439 1126 4177 439
Size: 687 3051 2420
Fragments arranged by size:
3051 2420 687
FspI TGC'GCA
Cuts at: 3939 3939
Size: 6158
HaeII r_GCGC'y
Cuts at: 606 1029 1167 1337 1748 1984 2277 2292 2403
Size: 423 138 170 411 236 293 15 111
Cuts at: 2403 2409 2478 2652 2678 2732 2844 3116 3592
Size: 6 69 174 26 54 112 272 476
Cuts at: 3592 3840 5070 5841 606
Size: 248 1230 771 923
Fragments arranged by size:
1230 923 771 476 423 411 293 272
248 236 174 170 138 112 111 69
54 26 15 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 102 134 232 264 307 339 404 436 967
Size: 32 98 32 43 32 65 32 531
Cuts at: 967 999 1045 1077 3010 3042 4000 4030 4032
Size: 32 46 32 1933 32 958 30 2
Cuts at: 4032 4062 102
Size: 30 2198
Fragments arranged by size:
2198 1933 958 531 98 65 46 43
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2162 3197 2162
Size: 1035 5123
KasI G'GCGC_C
Cuts at: 1980 3588 3836 1980
Size: 1608 248 4302
Fragments arranged by size:
4302 1608 248
KpnI G_GTAC'C
Cuts at: 3178 3178
Size: 6158
MluI A'CGCG_T
Cuts at: 2164 5591 2164
Size: 3427 2731
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2006 4917 5101 2006
Size: 2911 184 3063
Fragments arranged by size:
3063 2911 184
MscI TGG'CCA
Cuts at: 737 1303 2698 3919 737
Size: 566 1395 1221 2976
Fragments arranged by size:
2976 1395 1221 566
MslI CAynn'nnrTG
Cuts at: 4147 4274 4147
Size: 127 6031
MspA1I CmG'CkG
Cuts at: 235 1116 1545 2040 2061 2118 2217 2256 2721
Size: 881 429 495 21 57 99 39 465
Cuts at: 2721 2880 3016 3269 3583 3943 4727 4972 235
Size: 159 136 253 314 360 784 245 1421
Fragments arranged by size:
1421 881 784 495 465 429 360 314
253 245 159 136 99 57 39 21
NaeI GCC'GGC
Cuts at: 2692 3098 4339 2692
Size: 406 1241 4511
Fragments arranged by size:
4511 1241 406
NarI GG'CG_CC
Cuts at: 1981 3589 3837 1981
Size: 1608 248 4302
Fragments arranged by size:
4302 1608 248
NgoMIV G'CCGG_C
Cuts at: 2690 3096 4337 2690
Size: 406 1241 4511
Fragments arranged by size:
4511 1241 406
NspI r_CATG'y
Cuts at: 1110 1140 2616 3211 3711 5314 1110
Size: 30 1476 595 500 1603 1954
Fragments arranged by size:
1954 1603 1476 595 500 30
PasI CC'CwG_GG
Cuts at: 160 160
Size: 6158
PciI A'CATG_T
Cuts at: 5310 5310
Size: 6158
PflMI CCAn_nnn'nTGG
Cuts at: 2310 2986 2310
Size: 676 5482
PfoI T'CCnGG_A
Cuts at: 352 1508 2018 2129 352
Size: 1156 510 111 4381
Fragments arranged by size:
4381 1156 510 111
PmeI GTTT'AAAC
Cuts at: 3157 3157
Size: 6158
PmlI CAC'GTG
Cuts at: 456 456
Size: 6158
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 499 531 2131 2163 2917 2949 4604 4636 499
Size: 32 1600 32 754 32 1655 32 2021
Fragments arranged by size:
2021 1655 1600 754 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 130 1650 130
Size: 1520 4638
PspOMI G'GGCC_C
Cuts at: 494 1450 2598 494
Size: 956 1148 4054
Fragments arranged by size:
4054 1148 956
PstI C_TGCA'G
Cuts at: 503 515 1670 1732 2159 2249 2336 3205 3890
Size: 12 1155 62 427 90 87 869 685
Cuts at: 3890 503
Size: 2771
Fragments arranged by size:
2771 1155 869 685 427 90 87 62
12
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6158
PvuII CAG'CTG
Cuts at: 235 1545 2061 2256 2721 2880 3016 3583 3943
Size: 1310 516 195 465 159 136 567 360
Cuts at: 3943 235
Size: 2450
Fragments arranged by size:
2450 1310 567 516 465 360 195 159
136
RsrII CG'GwC_CG
Cuts at: 4353 4353
Size: 6158
SacI G_AGCT'C
Cuts at: 1378 1954 2854 3172 1378
Size: 576 900 318 4364
Fragments arranged by size:
4364 900 576 318
SalI G'TCGA_C
Cuts at: 2160 3195 2160
Size: 1035 5123
SanDI GG'GwC_CC
Cuts at: 130 1650 130
Size: 1520 4638
SapI GCTCTTCn'nnn_
Cuts at: 4181 4391 4181
Size: 210 5948
SbfI CC_TGCA'GG
Cuts at: 503 1670 3205 503
Size: 1167 1535 3456
Fragments arranged by size:
3456 1535 1167
ScaI AGT'ACT
Cuts at: 5675 5675
Size: 6158
SfcI C'TryA_G
Cuts at: 33 499 511 1666 1728 2155 2245 2332 3201
Size: 466 12 1155 62 427 90 87 869
Cuts at: 3201 3886 4854 5045 33
Size: 685 968 191 1146
Fragments arranged by size:
1155 1146 968 869 685 466 427 191
90 87 62 12
SfoI GGC'GCC
Cuts at: 1982 3590 3838 1982
Size: 1608 248 4302
Fragments arranged by size:
4302 1608 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6158
SmaI CCC'GGG
Cuts at: 3180 3180
Size: 6158
SmlI C'TyrA_G
Cuts at: 466 595 1075 1684 2497 3043 3313 4228 4942
Size: 129 480 609 813 546 270 915 714
Cuts at: 4942 5204 466
Size: 262 1420
Fragments arranged by size:
1420 915 813 714 609 546 480 270
262 129
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6158
SphI G_CATG'C
Cuts at: 1140 2616 3211 3711 1140
Size: 1476 595 500 3587
Fragments arranged by size:
3587 1476 595 500
StuI AGG'CCT
Cuts at: 958 1634 2413 958
Size: 676 779 4703
Fragments arranged by size:
4703 779 676
StyI C'CwwG_G
Cuts at: 998 2798 3116 3292 3646 998
Size: 1800 318 176 354 3510
Fragments arranged by size:
3510 1800 354 318 176
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1210 3741 3874 4333 4542 1210
Size: 2531 133 459 209 2826
Fragments arranged by size:
2826 2531 459 209 133
TatI w'GTAC_w
Cuts at: 485 5673 485
Size: 5188 970
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1544 1576 5675 5707 1544
Size: 32 4099 32 1995
Fragments arranged by size:
4099 1995 32 32
Tth111I GACn'n_nGTC
Cuts at: 2052 2963 3955 2052
Size: 911 992 4255
Fragments arranged by size:
4255 992 911
XbaI T'CTAG_A
Cuts at: 3189 3189
Size: 6158
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3653 3653
Size: 6158
XmaI C'CCGG_G
Cuts at: 3178 3178
Size: 6158
XmnI GAAnn'nnTTC
Cuts at: 2146 2146
Size: 6158
ZraI GAC'GTC
Cuts at: 3141 3141
Size: 6158
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AflII AgeI AleI AscI AvrII BaeI BcgI BfrBI
BmgBI BmtI BsiWI BspEI BsrGI BstEII BstZ17I ClaI
CspCI FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PshAI PsiI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI