Restriction map for
pF1KA0522
(Circular) (Six-base) MAPSORT of: pF1KA0522.seq Check: 9577 from: 1 to: 7577
pF1KA0522 7577 bp
With 182 enzymes: SgfI *
September 18, 2009 16:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 590 590
Size: 7577
AccI GT'mk_AC
Cuts at: 3018 4615 3018
Size: 1597 5980
Acc65I G'GTAC_C
Cuts at: 4593 4593
Size: 7577
AclI AA'CG_TT
Cuts at: 7417 7417
Size: 7577
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1629 2023 2081 2670 4260 5402 5834 6581 6849
Size: 394 58 589 1590 1142 432 747 268
Cuts at: 6849 1629
Size: 2357
Fragments arranged by size:
2357 1590 1142 747 589 432 394 268
58
AfeI AGC'GCT
Cuts at: 1051 1051
Size: 7577
AflIII A'CryG_T
Cuts at: 2558 2789 4929 6033 7010 2558
Size: 231 2140 1104 977 3125
Fragments arranged by size:
3125 2140 1104 977 231
AgeI A'CCGG_T
Cuts at: 541 2460 5965 541
Size: 1919 3505 2153
Fragments arranged by size:
3505 2153 1919
AhdI GACnn_n'nnGTC
Cuts at: 2949 2949
Size: 7577
AleI CACnn'nnGTG
Cuts at: 2461 2461
Size: 7577
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3553 3553
Size: 7577
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 409 441 1379 1411 409
Size: 32 938 32 6575
Fragments arranged by size:
6575 938 32 32
AlwNI CAG_nnn'CTG
Cuts at: 213 216 381 2059 2430 3259 3906 4882 6449
Size: 3 165 1678 371 829 647 976 1567
Cuts at: 6449 213
Size: 1341
Fragments arranged by size:
1678 1567 1341 976 829 647 371 165
3
ApaI G_GGCC'C
Cuts at: 128 958 1565 2031 2090 2123 3758 3937 4100
Size: 830 607 466 59 33 1635 179 163
Cuts at: 4100 4261 4324 4505 128
Size: 161 63 181 3200
Fragments arranged by size:
3200 1635 830 607 466 181 179 163
161 63 59 33
ApaLI G'TGCA_C
Cuts at: 6347 6347
Size: 7577
ApoI r'AATT_y
Cuts at: 828 2521 3131 3216 4581 6930 7186 828
Size: 1693 610 85 1365 2349 256 1219
Fragments arranged by size:
2349 1693 1365 1219 610 256 85
AseI AT'TA_AT
Cuts at: 20 20
Size: 7577
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7577
AvaI C'yCGr_G
Cuts at: 129 138 354 440 645 1735 1793 1834 2258
Size: 9 216 86 205 1090 58 41 424
Cuts at: 2258 2882 4597 129
Size: 624 1715 3109
Fragments arranged by size:
3109 1715 1090 624 424 216 205 86
58 41 9
AvrII C'CTAG_G
Cuts at: 2526 2526
Size: 7577
BamHI G'GATC_C
Cuts at: 142 4327 4602 142
Size: 4185 275 3117
Fragments arranged by size:
4185 3117 275
BanI G'GyrC_C
Cuts at: 469 754 1354 1366 1746 3048 4131 4593 5007
Size: 285 600 12 380 1302 1083 462 414
Cuts at: 5007 5255 5290 469
Size: 248 35 2756
Fragments arranged by size:
2756 1302 1083 600 462 414 380 285
248 35 12
BanII G_rGCy'C
Cuts at: 128 154 745 958 987 1138 1397 1565 2031
Size: 26 591 213 29 151 259 168 466
Cuts at: 2031 2090 2123 2212 3512 3727 3758 3937 4021
Size: 59 33 89 1300 215 31 179 84
Cuts at: 4021 4100 4261 4324 4348 4505 4591 5621 128
Size: 79 161 63 24 157 86 1030 2084
Fragments arranged by size:
2084 1300 1030 591 466 259 215 213
179 168 161 157 151 89 86 84
79 63 59 33 31 29 26 24
BbeI G_GCGC'C
Cuts at: 473 758 1750 5011 5259 473
Size: 285 992 3261 248 2791
Fragments arranged by size:
3261 2791 992 285 248
BbsI GAAGACnn'nnnn_
Cuts at: 1805 2879 3090 1805
Size: 1074 211 6292
Fragments arranged by size:
6292 1074 211
BbvCI CC'TCA_GC
Cuts at: 2500 4041 2500
Size: 1541 6036
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3008 3042 3328 3362 4551 4585 3008
Size: 34 286 34 1189 34 6000
Fragments arranged by size:
6000 1189 286 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2431 5475 6236 7577 2431
Size: 3044 761 1341 2431
Fragments arranged by size:
3044 2431 1341 761
BclI T'GATC_A
Cuts at: 5097 5097
Size: 7577
BglI GCCn_nnn'nGGC
Cuts at: 112 637 3863 3885 4124 112
Size: 525 3226 22 239 3565
Fragments arranged by size:
3565 3226 525 239 22
BglII A'GATC_T
Cuts at: 3149 5092 7129 3149
Size: 1943 2037 3597
Fragments arranged by size:
3597 2037 1943
BlpI GC'TnA_GC
Cuts at: 721 919 986 1224 1632 4689 721
Size: 198 67 238 408 3057 3609
Fragments arranged by size:
3609 3057 408 238 198 67
Bme1580I G_kGCm'C
Cuts at: 128 958 996 1371 1565 2031 2090 2123 3758
Size: 830 38 375 194 466 59 33 1635
Cuts at: 3758 3937 4100 4134 4261 4324 4505 5202 5295
Size: 179 163 34 127 63 181 697 93
Cuts at: 5295 6351 128
Size: 1056 1354
Fragments arranged by size:
1635 1354 1056 830 697 466 375 194
181 179 163 127 93 63 59 38
34 33
BmgBI CAC'GTC
Cuts at: 447 567 2559 447
Size: 120 1992 5465
Fragments arranged by size:
5465 1992 120
BmrI ACTGGGnnnn_n'
Cuts at: 387 567 2117 2126 3909 4081 4365 4520 4789
Size: 180 1550 9 1783 172 284 155 269
Cuts at: 4789 4870 5205 7349 387
Size: 81 335 2144 615
Fragments arranged by size:
2144 1783 1550 615 335 284 269 180
172 155 81 9
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1647 5944 1647
Size: 4297 3280
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 268 514 729 790 921 985 986 1138
Size: 69 246 215 61 131 64 1 152
Cuts at: 1138 1478 1615 2196 2506 3289 3412 3455 3502
Size: 340 137 581 310 783 123 43 47
Cuts at: 3502 3883 4169 4226 199
Size: 381 286 57 3550
Fragments arranged by size:
3550 783 581 381 340 310 286 246
215 152 137 131 123 69 64 61
57 47 43 1
Bpu10I CC'TnA_GC
Cuts at: 874 1144 1694 2500 4041 4262 874
Size: 270 550 806 1541 221 4189
Fragments arranged by size:
4189 1541 806 550 270 221
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1507 2224 4753 5632 6124 6422 1507
Size: 717 2529 879 492 298 2662
Fragments arranged by size:
2662 2529 879 717 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 382 2221 2879 3536 7281 382
Size: 1839 658 657 3745 678
Fragments arranged by size:
3745 1839 678 658 657
BsaAI yAC'GTr
Cuts at: 4930 4930
Size: 7577
BsaBI GATnn'nnATC
Cuts at: 6 5091 7128 6
Size: 5085 2037 455
Fragments arranged by size:
5085 2037 455
BsaHI Gr'CG_yC
Cuts at: 470 515 755 1747 5008 5256 5958 7454 470
Size: 45 240 992 3261 248 702 1496 593
Fragments arranged by size:
3261 1496 992 702 593 248 240 45
BsaWI w'CCGG_w
Cuts at: 541 2460 2618 2732 4766 5287 5965 6239 6386
Size: 1919 158 114 2034 521 678 274 147
Cuts at: 6386 6797 541
Size: 411 1321
Fragments arranged by size:
2034 1919 1321 678 521 411 274 158
147 114
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 103 133 409 439 478 508 732 762 1111
Size: 30 276 30 39 30 224 30 349
Cuts at: 1111 1141 1379 1409 1483 1513 1567 1597 2227
Size: 30 238 30 74 30 54 30 630
Cuts at: 2227 2257 2578 2608 3938 3968 103
Size: 30 321 30 1330 30 3712
Fragments arranged by size:
3712 1330 630 349 321 276 238 224
74 54 39 30 30 30 30 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 256 310 1048 1051 1054 1306 1588 2241 2583
Size: 54 738 3 3 252 282 653 342
Cuts at: 2583 2767 3691 3699 3789 256
Size: 184 924 8 90 4044
Fragments arranged by size:
4044 924 738 653 342 282 252 184
90 54 8 3 3
BseYI C'CCAG_C
Cuts at: 224 597 1589 1705 2052 2067 2073 2134 2330
Size: 373 992 116 347 15 6 61 196
Cuts at: 2330 2712 2800 3298 3962 3989 4010 4014 4232
Size: 382 88 498 664 27 21 4 218
Cuts at: 4232 4315 5002 5760 6337 6833 224
Size: 83 687 758 577 496 968
Fragments arranged by size:
992 968 758 687 664 577 498 496
382 373 347 218 196 116 88 83
61 27 21 15 6 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 307 568 2980 3376 4174 4474 292
Size: 15 261 2412 396 798 300 3395
Fragments arranged by size:
3395 2412 798 396 300 261 15
BsiEI CG_ry'CG
Cuts at: 104 5119 5165 6373 104
Size: 5015 46 1208 1308
Fragments arranged by size:
5015 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 745 880 2212 4591 5369 5559 6351 745
Size: 135 1332 2379 778 190 792 1971
Fragments arranged by size:
2379 1971 1332 792 778 190 135
BsmBI CGTCTCn'nnnn_
Cuts at: 2549 3705 2549
Size: 1156 6421
Bsp1286I G_dGCh'C
Cuts at: 128 154 745 880 958 987 996 1138 1371
Size: 26 591 135 78 29 9 142 233
Cuts at: 1371 1397 1565 2031 2090 2123 2212 3512 3727
Size: 26 168 466 59 33 89 1300 215
Cuts at: 3727 3758 3937 4021 4100 4134 4261 4324 4348
Size: 31 179 84 79 34 127 63 24
Cuts at: 4348 4505 4591 5202 5295 5369 5559 5621 6351
Size: 157 86 611 93 74 190 62 730
Cuts at: 6351 128
Size: 1354
Fragments arranged by size:
1354 1300 730 611 591 466 233 215
190 179 168 157 142 135 127 93
89 86 84 79 78 74 63 62
59 34 33 31 29 26 26 24
9
BspEI T'CCGG_A
Cuts at: 2618 2732 6797 2618
Size: 114 4065 3398
Fragments arranged by size:
4065 3398 114
BspHI T'CATG_A
Cuts at: 6753 6753
Size: 7577
BspMI ACCTGCnnnn'nnnn_
Cuts at: 182 296 389 590 1100 3341 4627 4841 5143
Size: 114 93 201 510 2241 1286 214 302
Cuts at: 5143 5524 182
Size: 381 2235
Fragments arranged by size:
2241 2235 1286 510 381 302 214 201
114 93
BsrBI CCG'CTC
Cuts at: 50 510 528 711 1212 1934 2439 2490 5869
Size: 460 18 183 501 722 505 51 3379
Cuts at: 5869 5923 7407 7572 50
Size: 54 1484 165 55
Fragments arranged by size:
3379 1484 722 505 501 460 183 165
55 54 51 18
BsrDI GCAATG_nn'
Cuts at: 3344 5489 3344
Size: 2145 5432
BsrFI r'CCGG_y
Cuts at: 541 636 757 914 1379 1730 2287 2460 2504
Size: 95 121 157 465 351 557 173 44
Cuts at: 2504 2534 3311 4118 5575 5756 5965 541
Size: 30 777 807 1457 181 209 2153
Fragments arranged by size:
2153 1457 807 777 557 465 351 209
181 173 157 121 95 44 30
BsrGI T'GTAC_A
Cuts at: 2792 4217 2792
Size: 1425 6152
BssHII G'CGCG_C
Cuts at: 341 341
Size: 7577
BssSI C'ACGA_G
Cuts at: 589 2719 3187 5848 6206 589
Size: 2130 468 2661 358 1960
Fragments arranged by size:
2661 2130 1960 468 358
BstAPI GCAn_nnn'nTGC
Cuts at: 213 4954 213
Size: 4741 2836
BstBI TT'CG_AA
Cuts at: 5938 5938
Size: 7577
BstEII G'GTnAC_C
Cuts at: 3123 3123
Size: 7577
BstXI CCAn_nnnn'nTGG
Cuts at: 2130 2604 3847 4081 2130
Size: 474 1243 234 5626
Fragments arranged by size:
5626 1243 474 234
BstYI r'GATC_y
Cuts at: 1 142 2601 3054 3149 4327 4602 5069 5092
Size: 141 2459 453 95 1178 275 467 23
Cuts at: 5092 5427 5673 6685 6771 6783 7129 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
2459 1178 1012 467 453 449 346 335
275 246 141 95 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1021 1021
Size: 7577
BtgI C'CryG_G
Cuts at: 107 164 312 336 694 703 726 1358 1916
Size: 57 148 24 358 9 23 632 558
Cuts at: 1916 1992 2597 3858 4080 4479 4565 107
Size: 76 605 1261 222 399 86 3119
Fragments arranged by size:
3119 1261 632 605 558 399 358 222
148 86 76 57 24 23 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3518 4048 5527 5708 5871 3518
Size: 530 1479 181 163 5224
Fragments arranged by size:
5224 1479 530 181 163
BtsI GCAGTG_nn'
Cuts at: 873 4207 4820 873
Size: 3334 613 3630
Fragments arranged by size:
3630 3334 613
ClaI AT'CG_AT
Cuts at: 1427 1427
Size: 7577
DraI TTT'AAA
Cuts at: 4576 4576
Size: 7577
DrdI GACnn_nn'nnGTC
Cuts at: 5283 6141 5283
Size: 858 6719
EaeI y'GGCC_r
Cuts at: 697 794 3849 4120 4176 4333 5162 5336 5727
Size: 97 3055 271 56 157 829 174 391
Cuts at: 5727 5754 7099 697
Size: 27 1345 1175
Fragments arranged by size:
3055 1345 1175 829 391 271 174 157
97 56 27
EagI C'GGCC_G
Cuts at: 5162 5162
Size: 7577
EarI CTCTTCn'nnn_
Cuts at: 2259 2401 3109 3214 3807 3980 5600 5810 2259
Size: 142 708 105 593 173 1620 210 4026
Fragments arranged by size:
4026 1620 708 593 210 173 142 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 776 1171 3644 5785 6095 6241 7387 776
Size: 395 2473 2141 310 146 1146 966
Fragments arranged by size:
2473 2141 1146 966 395 310 146
EcoICRI GAG'CTC
Cuts at: 743 2210 4589 743
Size: 1467 2379 3731
Fragments arranged by size:
3731 2379 1467
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 268 514 729 790 921 985 986 1138
Size: 69 246 215 61 131 64 1 152
Cuts at: 1138 1478 1615 1629 2023 2081 2196 2506 2670
Size: 340 137 14 394 58 115 310 164
Cuts at: 2670 3289 3412 3455 3502 3883 4169 4226 4260
Size: 619 123 43 47 381 286 57 34
Cuts at: 4260 5402 5834 6581 6849 199
Size: 1142 432 747 268 927
Fragments arranged by size:
1142 927 747 619 432 394 381 340
310 286 268 246 215 164 152 137
131 123 115 69 64 61 58 57
47 43 34 14 1
EcoNI CCTnn'n_nnAGG
Cuts at: 292 292
Size: 7577
EcoO109I rG'GnC_Cy
Cuts at: 124 125 330 954 955 1059 1561 1831 1997
Size: 1 205 624 1 104 502 270 166
Cuts at: 1997 2027 2028 2086 2087 3754 3755 3903 3934
Size: 30 1 58 1 1667 1 148 31
Cuts at: 3934 4096 4097 4257 4258 4320 4321 4502 4716
Size: 162 1 160 1 62 1 181 214
Cuts at: 4716 124
Size: 2985
Fragments arranged by size:
2985 1667 624 502 270 214 205 181
166 162 160 148 104 62 58 31
30 1 1 1 1 1 1 1
1
EcoRI G'AATT_C
Cuts at: 828 2521 3216 4581 828
Size: 1693 695 1365 3824
Fragments arranged by size:
3824 1693 1365 695
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3168 3774 5596 3168
Size: 606 1822 5149
Fragments arranged by size:
5149 1822 606
FseI GG_CCGG'CC
Cuts at: 1734 4122 1734
Size: 2388 5189
FspI TGC'GCA
Cuts at: 5358 5358
Size: 7577
HaeII r_GCGC'y
Cuts at: 473 758 1053 1750 5011 5259 6281 7260 473
Size: 285 295 697 3261 248 1022 979 790
Fragments arranged by size:
3261 1022 979 790 697 295 285 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 103 135 141 173 732 764 1483 1515 1567
Size: 32 6 32 559 32 719 32 52
Cuts at: 1567 1599 1930 1962 2578 2610 2935 2967 3636
Size: 32 331 32 616 32 325 32 669
Cuts at: 3636 3668 3874 3906 5419 5449 5451 5481 103
Size: 32 206 32 1513 30 2 30 2199
Fragments arranged by size:
2199 1513 719 669 616 559 331 325
206 52 32 32 32 32 32 32
32 32 32 32 30 30 6 2
HincII GTy'rAC
Cuts at: 2862 3019 4616 2862
Size: 157 1597 5823
Fragments arranged by size:
5823 1597 157
KasI G'GCGC_C
Cuts at: 469 754 1746 5007 5255 469
Size: 285 992 3261 248 2791
Fragments arranged by size:
3261 2791 992 285 248
KpnI G_GTAC'C
Cuts at: 4597 4597
Size: 7577
MluI A'CGCG_T
Cuts at: 7010 7010
Size: 7577
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1289 2122 2543 2964 3353 3365 3728 6248 6432
Size: 833 421 421 389 12 363 2520 184
Cuts at: 6432 1289
Size: 2434
Fragments arranged by size:
2520 2434 833 421 421 389 363 184
12
MscI TGG'CCA
Cuts at: 3851 5338 3851
Size: 1487 6090
MslI CAynn'nnrTG
Cuts at: 1273 2461 3845 4079 5566 5693 1273
Size: 1188 1384 234 1487 127 3157
Fragments arranged by size:
3157 1487 1384 1188 234 127
MspA1I CmG'CkG
Cuts at: 213 228 258 306 314 696 705 720 883
Size: 15 30 48 8 382 9 15 163
Cuts at: 883 927 990 1593 1962 2313 2694 3090 4014
Size: 44 63 603 369 351 381 396 924
Cuts at: 4014 4488 4688 5002 5362 6375 6620 213
Size: 474 200 314 360 1013 245 1170
Fragments arranged by size:
1170 1013 924 603 474 396 382 381
369 360 351 314 245 200 163 63
48 44 30 15 15 9 8
NaeI GCC'GGC
Cuts at: 638 759 1732 2506 4120 5758 638
Size: 121 973 774 1614 1638 2457
Fragments arranged by size:
2457 1638 1614 973 774 121
NarI GG'CG_CC
Cuts at: 470 755 1747 5008 5256 470
Size: 285 992 3261 248 2791
Fragments arranged by size:
3261 2791 992 285 248
NcoI C'CATG_G
Cuts at: 107 1358 1992 2597 4080 4479 107
Size: 1251 634 605 1483 399 3205
Fragments arranged by size:
3205 1483 1251 634 605 399
NdeI CA'TA_TG
Cuts at: 1401 1401
Size: 7577
NgoMIV G'CCGG_C
Cuts at: 636 757 1730 2504 4118 5756 636
Size: 121 973 774 1614 1638 2457
Fragments arranged by size:
2457 1638 1614 973 774 121
NruI TCG'CGA
Cuts at: 611 611
Size: 7577
NspI r_CATG'y
Cuts at: 1245 1272 2793 3204 3913 4630 5130 6037 1245
Size: 27 1521 411 709 717 500 907 2785
Fragments arranged by size:
2785 1521 907 717 709 500 411 27
PasI CC'CwG_GG
Cuts at: 603 980 1725 4093 603
Size: 377 745 2368 4087
Fragments arranged by size:
4087 2368 745 377
PciI A'CATG_T
Cuts at: 2789 6033 2789
Size: 3244 4333
PflMI CCAn_nnn'nTGG
Cuts at: 2664 2664
Size: 7577
PfoI T'CCnGG_A
Cuts at: 530 736 2483 3305 530
Size: 206 1747 822 4802
Fragments arranged by size:
4802 1747 822 206
PmeI GTTT'AAAC
Cuts at: 4576 4576
Size: 7577
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 379 409 411 441 1379 1411 6716 6748 379
Size: 30 2 30 938 32 5305 32 1208
Fragments arranged by size:
5305 1208 938 32 32 30 30 2
PpuMI rG'GwC_Cy
Cuts at: 330 1831 1997 330
Size: 1501 166 5910
Fragments arranged by size:
5910 1501 166
PspOMI G'GGCC_C
Cuts at: 124 954 1561 2027 2086 2119 3754 3933 4096
Size: 830 607 466 59 33 1635 179 163
Cuts at: 4096 4257 4320 4501 124
Size: 161 63 181 3200
Fragments arranged by size:
3200 1635 830 607 466 181 179 163
161 63 59 33
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1303 1335 1303
Size: 32 7545
PstI C_TGCA'G
Cuts at: 1097 1987 2242 3255 4624 5309 1097
Size: 890 255 1013 1369 685 3365
Fragments arranged by size:
3365 1369 1013 890 685 255
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7577
PvuII CAG'CTG
Cuts at: 213 258 306 720 927 1593 1962 4014 5002
Size: 45 48 414 207 666 369 2052 988
Cuts at: 5002 5362 213
Size: 360 2428
Fragments arranged by size:
2428 2052 988 666 414 369 360 207
48 45
RsrII CG'GwC_CG
Cuts at: 5772 5772
Size: 7577
SacI G_AGCT'C
Cuts at: 745 2212 4591 745
Size: 1467 2379 3731
Fragments arranged by size:
3731 2379 1467
SacII CC_GC'GG
Cuts at: 315 697 706 315
Size: 382 9 7186
Fragments arranged by size:
7186 382 9
SalI G'TCGA_C
Cuts at: 3017 4614 3017
Size: 1597 5980
SanDI GG'GwC_CC
Cuts at: 330 1831 1997 330
Size: 1501 166 5910
Fragments arranged by size:
5910 1501 166
SapI GCTCTTCn'nnn_
Cuts at: 3214 5600 5810 3214
Size: 2386 210 4981
Fragments arranged by size:
4981 2386 210
SbfI CC_TGCA'GG
Cuts at: 1097 4624 1097
Size: 3527 4050
ScaI AGT'ACT
Cuts at: 7094 7094
Size: 7577
SexAI A'CCwGG_T
Cuts at: 3258 3258
Size: 7577
SfcI C'TryA_G
Cuts at: 33 1093 1983 2238 3251 4620 5305 6298 6489
Size: 1060 890 255 1013 1369 685 993 191
Cuts at: 6489 33
Size: 1121
Fragments arranged by size:
1369 1121 1060 1013 993 890 685 255
191
SfoI GGC'GCC
Cuts at: 471 756 1748 5009 5257 471
Size: 285 992 3261 248 2791
Fragments arranged by size:
3261 2791 992 285 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7577
SgrAI Cr'CCGG_yG
Cuts at: 757 2460 757
Size: 1703 5874
SmaI CCC'GGG
Cuts at: 131 140 356 442 647 1795 1836 4599 131
Size: 9 216 86 205 1148 41 2763 3109
Fragments arranged by size:
3109 2763 1148 216 205 86 41 9
SmlI C'TyrA_G
Cuts at: 1486 2203 4732 5647 6139 6401 1486
Size: 717 2529 915 492 262 2662
Fragments arranged by size:
2662 2529 915 717 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7577
SphI G_CATG'C
Cuts at: 3913 4630 5130 3913
Size: 717 500 6360
Fragments arranged by size:
6360 717 500
SrfI GCCC'GGGC
Cuts at: 356 356
Size: 7577
StuI AGG'CCT
Cuts at: 2498 3790 2498
Size: 1292 6285
StyI C'CwwG_G
Cuts at: 107 1055 1358 1992 2526 2597 3443 3924 4080
Size: 948 303 634 534 71 846 481 156
Cuts at: 4080 4112 4479 4538 4711 5065 107
Size: 32 367 59 173 354 2619
Fragments arranged by size:
2619 948 846 634 534 481 367 354
303 173 156 71 59 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 286 4149 4301 4410 4482 5160 5293 5752 5961
Size: 3863 152 109 72 678 133 459 209
Cuts at: 5961 286
Size: 1902
Fragments arranged by size:
3863 1902 678 459 209 152 133 109
72
TatI w'GTAC_w
Cuts at: 2792 3680 4217 7092 2792
Size: 888 537 2875 3277
Fragments arranged by size:
3277 2875 888 537
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3938 3970 7094 7126 3938
Size: 32 3124 32 4389
Fragments arranged by size:
4389 3124 32 32
Tth111I GACn'n_nGTC
Cuts at: 5374 5374
Size: 7577
XbaI T'CTAG_A
Cuts at: 4608 4608
Size: 7577
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 724 945 3844 3859 5072 724
Size: 221 2899 15 1213 3229
Fragments arranged by size:
3229 2899 1213 221 15
XmaI C'CCGG_G
Cuts at: 129 138 354 440 645 1793 1834 4597 129
Size: 9 216 86 205 1148 41 2763 3109
Fragments arranged by size:
3109 2763 1148 216 205 86 41 9
XmnI GAAnn'nnTTC
Cuts at: 2545 2545
Size: 7577
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NruI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI SrfI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AscI BaeI BfrBI BmtI BsiWI BsmI
BstZ17I CspCI DraIII EcoRV FspAI HindIII HpaI MfeI
NheI NotI NsiI PacI PmlI PshAI PsiI PspXI
SfiI SnaBI SspI SwaI XhoI ZraI