Restriction map for
pF1KA0523
(Circular) (Six-base) MAPSORT of: pF1KA0523.seq Check: 723 from: 1 to: 4838
pF1KA0523 4838 bp
With 182 enzymes: SgfI *
September 18, 2009 16:59 ..
AccI GT'mk_AC
Cuts at: 659 1097 1876 659
Size: 438 779 3621
Fragments arranged by size:
3621 779 438
Acc65I G'GTAC_C
Cuts at: 1854 1854
Size: 4838
AclI AA'CG_TT
Cuts at: 4678 4678
Size: 4838
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 537 2663 3095 3842 4110 537
Size: 2126 432 747 268 1265
Fragments arranged by size:
2126 1265 747 432 268
AflIII A'CryG_T
Cuts at: 1199 1634 2190 3294 4271 1199
Size: 435 556 1104 977 1766
Fragments arranged by size:
1766 1104 977 556 435
AgeI A'CCGG_T
Cuts at: 3226 3226
Size: 4838
AleI CACnn'nnGTG
Cuts at: 1343 1343
Size: 4838
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 220 337 1615 220
Size: 117 1278 3443
Fragments arranged by size:
3443 1278 117
AlwNI CAG_nnn'CTG
Cuts at: 159 983 1077 2143 3710 159
Size: 824 94 1066 1567 1287
Fragments arranged by size:
1567 1287 1066 824 94
ApaI G_GGCC'C
Cuts at: 244 502 244
Size: 258 4580
ApaLI G'TGCA_C
Cuts at: 1570 3608 1570
Size: 2038 2800
ApoI r'AATT_y
Cuts at: 1420 1842 4191 4447 1420
Size: 422 2349 256 1811
Fragments arranged by size:
2349 1811 422 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4838
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4838
AvaI C'yCGr_G
Cuts at: 245 332 502 936 1858 245
Size: 87 170 434 922 3225
Fragments arranged by size:
3225 922 434 170 87
BamHI G'GATC_C
Cuts at: 1863 1863
Size: 4838
BanI G'GyrC_C
Cuts at: 249 535 1211 1442 1599 1854 2268 2516 2551
Size: 286 676 231 157 255 414 248 35
Cuts at: 2551 249
Size: 2536
Fragments arranged by size:
2536 676 414 286 255 248 231 157
35
BanII G_rGCy'C
Cuts at: 244 321 379 502 766 826 1727 1852 2882
Size: 77 58 123 264 60 901 125 1030
Cuts at: 2882 244
Size: 2200
Fragments arranged by size:
2200 1030 901 264 125 123 77 60
58
BbeI G_GCGC'C
Cuts at: 2272 2520 2272
Size: 248 4590
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 313 347 1102 1136 313
Size: 34 755 34 4015
Fragments arranged by size:
4015 755 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2736 3497 4838 2736
Size: 761 1341 2736
Fragments arranged by size:
2736 1341 761
BclI T'GATC_A
Cuts at: 1751 2358 1751
Size: 607 4231
BglI GCCn_nnn'nGGC
Cuts at: 286 286
Size: 4838
BglII A'GATC_T
Cuts at: 2353 4390 2353
Size: 2037 2801
BlpI GC'TnA_GC
Cuts at: 644 1950 644
Size: 1306 3532
Bme1580I G_kGCm'C
Cuts at: 244 252 502 1209 1445 1574 1604 1829 2463
Size: 8 250 707 236 129 30 225 634
Cuts at: 2463 2556 3612 244
Size: 93 1056 1470
Fragments arranged by size:
1470 1056 707 634 250 236 225 129
93 30 8
BmgBI CAC'GTC
Cuts at: 1530 1530
Size: 4838
BmrI ACTGGGnnnn_n'
Cuts at: 2050 2131 2466 4610 2050
Size: 81 335 2144 2278
Fragments arranged by size:
2278 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 780 3205 780
Size: 2425 2413
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 115 810 115
Size: 695 4143
Bpu10I CC'TnA_GC
Cuts at: 1721 1721
Size: 4838
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 624 1528 2014 2893 3385 3683 624
Size: 904 486 879 492 298 1779
Fragments arranged by size:
1779 904 879 492 486 298
BsaI GGTCTCn'nnnn_
Cuts at: 4542 4542
Size: 4838
BsaAI yAC'GTr
Cuts at: 1202 2191 1202
Size: 989 3849
BsaBI GATnn'nnATC
Cuts at: 6 2352 4389 6
Size: 2346 2037 455
Fragments arranged by size:
2346 2037 455
BsaHI Gr'CG_yC
Cuts at: 2269 2517 3219 4715 2269
Size: 248 702 1496 2392
Fragments arranged by size:
2392 1496 702 248
BsaWI w'CCGG_w
Cuts at: 413 1391 1766 2027 2548 3226 3500 3647 4058
Size: 978 375 261 521 678 274 147 411
Cuts at: 4058 413
Size: 1193
Fragments arranged by size:
1193 978 678 521 411 375 274 261
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 775 805 775
Size: 30 4808
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1594 1663 1594
Size: 69 4769
BseYI C'CCAG_C
Cuts at: 291 354 895 983 2263 3021 3598 4094 291
Size: 63 541 88 1280 758 577 496 1035
Fragments arranged by size:
1280 1035 758 577 541 496 88 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 307
Size: 4838
BsiEI CG_ry'CG
Cuts at: 104 1704 2380 2426 3634 104
Size: 1600 676 46 1208 1308
Fragments arranged by size:
1600 1308 1208 676 46
BsiHKAI G_wGCw'C
Cuts at: 1574 1852 2630 2820 3612 1574
Size: 278 778 190 792 2800
Fragments arranged by size:
2800 792 778 278 190
BsmI GAATG_Cn'
Cuts at: 994 994
Size: 4838
Bsp1286I G_dGCh'C
Cuts at: 244 252 321 379 502 766 826 1209 1445
Size: 8 69 58 123 264 60 383 236
Cuts at: 1445 1574 1604 1727 1829 1852 2463 2556 2630
Size: 129 30 123 102 23 611 93 74
Cuts at: 2630 2820 2882 3612 244
Size: 190 62 730 1470
Fragments arranged by size:
1470 730 611 383 264 236 190 129
123 123 102 93 74 69 62 60
58 30 23 8
BspEI T'CCGG_A
Cuts at: 413 1391 1766 4058 413
Size: 978 375 2292 1193
Fragments arranged by size:
2292 1193 978 375
BspHI T'CATG_A
Cuts at: 467 4014 467
Size: 3547 1291
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1031 1888 2102 2404 2785 1031
Size: 857 214 302 381 3084
Fragments arranged by size:
3084 857 381 302 214
BsrBI CCG'CTC
Cuts at: 50 648 1653 3130 3184 4668 4833 50
Size: 598 1005 1477 54 1484 165 55
Fragments arranged by size:
1484 1477 1005 598 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2750 2750
Size: 4838
BsrFI r'CCGG_y
Cuts at: 193 664 701 785 1703 2836 3017 3226 193
Size: 471 37 84 918 1133 181 209 1805
Fragments arranged by size:
1805 1133 918 471 209 181 84 37
BsrGI T'GTAC_A
Cuts at: 1126 1126
Size: 4838
BssSI C'ACGA_G
Cuts at: 568 1339 1512 3109 3467 568
Size: 771 173 1597 358 1939
Fragments arranged by size:
1939 1597 771 358 173
BstAPI GCAn_nnn'nTGC
Cuts at: 270 2215 270
Size: 1945 2893
BstBI TT'CG_AA
Cuts at: 3199 3199
Size: 4838
BstXI CCAn_nnnn'nTGG
Cuts at: 1345 1345
Size: 4838
BstYI r'GATC_y
Cuts at: 1 1863 2330 2353 2688 2934 3946 4032 4044
Size: 1862 467 23 335 246 1012 86 12
Cuts at: 4044 4390 1
Size: 346 449
Fragments arranged by size:
1862 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1064 1064
Size: 4838
BtgI C'CryG_G
Cuts at: 107 284 852 923 1034 107
Size: 177 568 71 111 3911
Fragments arranged by size:
3911 568 177 111 71
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2788 2969 3132 2788
Size: 181 163 4494
Fragments arranged by size:
4494 181 163
BtsI GCAGTG_nn'
Cuts at: 624 2081 624
Size: 1457 3381
DraI TTT'AAA
Cuts at: 1837 1837
Size: 4838
DrdI GACnn_nn'nnGTC
Cuts at: 334 2544 3402 334
Size: 2210 858 1770
Fragments arranged by size:
2210 1770 858
EaeI y'GGCC_r
Cuts at: 110 278 564 968 1053 1486 1701 2423 2597
Size: 168 286 404 85 433 215 722 174
Cuts at: 2597 2988 3015 4360 110
Size: 391 27 1345 588
Fragments arranged by size:
1345 722 588 433 404 391 286 215
174 168 85 27
EagI C'GGCC_G
Cuts at: 1701 2423 1701
Size: 722 4116
EarI CTCTTCn'nnn_
Cuts at: 725 1468 2861 3071 725
Size: 743 1393 210 2492
Fragments arranged by size:
2492 1393 743 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 133 697 852 1323 3046 3356 3502 4648 133
Size: 564 155 471 1723 310 146 1146 323
Fragments arranged by size:
1723 1146 564 471 323 310 155 146
EcoICRI GAG'CTC
Cuts at: 1850 1850
Size: 4838
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 115 537 810 2663 3095 3842 4110 115
Size: 422 273 1853 432 747 268 843
Fragments arranged by size:
1853 843 747 432 422 273 268
EcoNI CCTnn'n_nnAGG
Cuts at: 907 907
Size: 4838
EcoO109I rG'GnC_Cy
Cuts at: 240 241 499 1059 1406 1977 240
Size: 1 258 560 347 571 3101
Fragments arranged by size:
3101 571 560 347 258 1
EcoRI G'AATT_C
Cuts at: 1420 1842 1420
Size: 422 4416
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2857 2857
Size: 4838
FspI TGC'GCA
Cuts at: 2619 2619
Size: 4838
HaeII r_GCGC'y
Cuts at: 292 1710 2272 2520 3542 4521 292
Size: 1418 562 248 1022 979 609
Fragments arranged by size:
1418 1022 979 609 562 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 775 807 1307 1339 2680 2710 2712 2742 775
Size: 32 500 32 1341 30 2 30 2871
Fragments arranged by size:
2871 1341 500 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1500 1877 1500
Size: 377 4461
HindIII A'AGCT_T
Cuts at: 1179 1179
Size: 4838
KasI G'GCGC_C
Cuts at: 2268 2516 2268
Size: 248 4590
KpnI G_GTAC'C
Cuts at: 1858 1858
Size: 4838
MluI A'CGCG_T
Cuts at: 4271 4271
Size: 4838
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 150 888 3509 3693 150
Size: 738 2621 184 1295
Fragments arranged by size:
2621 1295 738 184
MscI TGG'CCA
Cuts at: 112 566 970 1055 2599 112
Size: 454 404 85 1544 2351
Fragments arranged by size:
2351 1544 454 404 85
MslI CAynn'nnrTG
Cuts at: 1343 2827 2954 1343
Size: 1484 127 3227
Fragments arranged by size:
3227 1484 127
MspA1I CmG'CkG
Cuts at: 216 407 452 712 854 1459 1949 2263 2623
Size: 191 45 260 142 605 490 314 360
Cuts at: 2623 3636 3881 216
Size: 1013 245 1173
Fragments arranged by size:
1173 1013 605 490 360 314 260 245
191 142 45
NaeI GCC'GGC
Cuts at: 666 1705 3019 666
Size: 1039 1314 2485
Fragments arranged by size:
2485 1314 1039
NarI GG'CG_CC
Cuts at: 2269 2517 2269
Size: 248 4590
NcoI C'CATG_G
Cuts at: 107 1034 107
Size: 927 3911
NgoMIV G'CCGG_C
Cuts at: 664 1703 3017 664
Size: 1039 1314 2485
Fragments arranged by size:
2485 1314 1039
NspI r_CATG'y
Cuts at: 369 889 1228 1891 2391 3298 369
Size: 520 339 663 500 907 1909
Fragments arranged by size:
1909 907 663 520 500 339
PasI CC'CwG_GG
Cuts at: 480 495 1814 480
Size: 15 1319 3504
Fragments arranged by size:
3504 1319 15
PciI A'CATG_T
Cuts at: 3294 3294
Size: 4838
PfoI T'CCnGG_A
Cuts at: 826 1532 826
Size: 706 4132
PmeI GTTT'AAAC
Cuts at: 1837 1837
Size: 4838
PmlI CAC'GTG
Cuts at: 1202 1202
Size: 4838
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3977 4009 3977
Size: 32 4806
PpuMI rG'GwC_Cy
Cuts at: 1059 1406 1059
Size: 347 4491
PspOMI G'GGCC_C
Cuts at: 240 498 240
Size: 258 4580
PspXI vC'TCGA_Gb
Cuts at: 332 332
Size: 4838
PstI C_TGCA'G
Cuts at: 215 266 374 818 1885 2570 215
Size: 51 108 444 1067 685 2483
Fragments arranged by size:
2483 1067 685 444 108 51
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4838
PvuII CAG'CTG
Cuts at: 1459 2263 2623 1459
Size: 804 360 3674
Fragments arranged by size:
3674 804 360
RsrII CG'GwC_CG
Cuts at: 839 3033 839
Size: 2194 2644
SacI G_AGCT'C
Cuts at: 1852 1852
Size: 4838
SacII CC_GC'GG
Cuts at: 855 855
Size: 4838
SalI G'TCGA_C
Cuts at: 1875 1875
Size: 4838
SapI GCTCTTCn'nnn_
Cuts at: 1468 2861 3071 1468
Size: 1393 210 3235
Fragments arranged by size:
3235 1393 210
SbfI CC_TGCA'GG
Cuts at: 1885 1885
Size: 4838
ScaI AGT'ACT
Cuts at: 4355 4355
Size: 4838
SfcI C'TryA_G
Cuts at: 33 211 262 370 814 1881 2566 3559 3750
Size: 178 51 108 444 1067 685 993 191
Cuts at: 3750 33
Size: 1121
Fragments arranged by size:
1121 1067 993 685 444 191 178 108
51
SfoI GGC'GCC
Cuts at: 2270 2518 2270
Size: 248 4590
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4838
SmaI CCC'GGG
Cuts at: 247 1860 247
Size: 1613 3225
SmlI C'TyrA_G
Cuts at: 332 603 1543 1993 2908 3400 3662 332
Size: 271 940 450 915 492 262 1508
Fragments arranged by size:
1508 940 915 492 450 271 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4838
SphI G_CATG'C
Cuts at: 1228 1891 2391 1228
Size: 663 500 3675
Fragments arranged by size:
3675 663 500
StyI C'CwwG_G
Cuts at: 107 965 1034 1972 2326 107
Size: 858 69 938 354 2619
Fragments arranged by size:
2619 938 858 354 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 346 2421 2554 3013 3222 346
Size: 2075 133 459 209 1962
Fragments arranged by size:
2075 1962 459 209 133
TatI w'GTAC_w
Cuts at: 1126 4353 1126
Size: 3227 1611
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 875 907 4355 4387 875
Size: 32 3448 32 1326
Fragments arranged by size:
3448 1326 32 32
Tth111I GACn'n_nGTC
Cuts at: 1495 2635 1495
Size: 1140 3698
XbaI T'CTAG_A
Cuts at: 1869 1869
Size: 4838
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 921 1164 2333 921
Size: 243 1169 3426
Fragments arranged by size:
3426 1169 243
XhoI C'TCGA_G
Cuts at: 332 332
Size: 4838
XmaI C'CCGG_G
Cuts at: 245 1858 245
Size: 1613 3225
XmnI GAAnn'nnTTC
Cuts at: 958 958
Size: 4838
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BaeI BbsI BbvCI BfrBI BmtI BsiWI BsmBI BssHII
BstEII BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PflMI PshAI PsiI PsrI SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI ZraI