Restriction map for
pF1KA0530
(Circular) (Six-base) MAPSORT of: pF1KA0530.seq Check: 4683 from: 1 to: 9905
pF1KA0530 9905 bp
With 182 enzymes: SgfI *
January 9, 2009 11:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2855 2855
Size: 9905
AatII G_ACGT'C
Cuts at: 3391 3391
Size: 9905
AccI GT'mk_AC
Cuts at: 179 987 5495 6943 179
Size: 808 4508 1448 3141
Fragments arranged by size:
4508 3141 1448 808
Acc65I G'GTAC_C
Cuts at: 6921 6921
Size: 9905
AclI AA'CG_TT
Cuts at: 6679 9745 6679
Size: 3066 6839
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1274 1316 1334 2703 3481 3929 3992 4103 4235
Size: 42 18 1369 778 448 63 111 132
Cuts at: 4235 4679 4904 5342 5354 5897 6538 6694 6769
Size: 444 225 438 12 543 641 156 75
Cuts at: 6769 7730 8162 8515 9177 1274
Size: 961 432 353 662 2002
Fragments arranged by size:
2002 1369 961 778 662 641 543 448
444 438 432 353 225 156 132 111
75 63 42 18 12
AfeI AGC'GCT
Cuts at: 868 868
Size: 9905
AflII C'TTAA_G
Cuts at: 1924 1924
Size: 9905
AflIII A'CryG_T
Cuts at: 4588 6086 6088 7257 9057 9338 4588
Size: 1498 2 1169 1800 281 5155
Fragments arranged by size:
5155 1800 1498 1169 281 2
AgeI A'CCGG_T
Cuts at: 2208 2208
Size: 9905
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 6074 6074
Size: 9905
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3562 3594 3562
Size: 32 9873
AlwNI CAG_nnn'CTG
Cuts at: 760 4390 6397 7210 8648 760
Size: 3630 2007 813 1438 2017
Fragments arranged by size:
3630 2017 2007 1438 813
ApaI G_GGCC'C
Cuts at: 2782 2782
Size: 9905
ApaLI G'TGCA_C
Cuts at: 8743 8743
Size: 9905
ApoI r'AATT_y
Cuts at: 379 431 769 819 1094 1737 1749 1870 2020
Size: 52 338 50 275 643 12 121 150
Cuts at: 2020 2427 2851 3027 3522 3843 3930 3951 4029
Size: 407 424 176 495 321 87 21 78
Cuts at: 4029 4247 4505 4558 4611 5118 5242 5273 6606
Size: 218 258 53 53 507 124 31 1333
Cuts at: 6606 6909 9258 9514 379
Size: 303 2349 256 770
Fragments arranged by size:
2349 1333 770 643 507 495 424 407
338 321 303 275 258 256 218 176
150 124 121 87 78 53 53 52
50 31 21 12
AseI AT'TA_AT
Cuts at: 20 6205 20
Size: 6185 3720
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9905
AvaI C'yCGr_G
Cuts at: 6925 6925
Size: 9905
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 6077 6110 6077
Size: 33 9872
BamHI G'GATC_C
Cuts at: 6595 6930 6595
Size: 335 9570
BanI G'GyrC_C
Cuts at: 3244 6921 7335 7583 7618 3244
Size: 3677 414 248 35 5531
Fragments arranged by size:
5531 3677 414 248 35
BanII G_rGCy'C
Cuts at: 1460 2782 6484 6919 7949 1460
Size: 1322 3702 435 1030 3416
Fragments arranged by size:
3702 3416 1322 1030 435
BbeI G_GCGC'C
Cuts at: 7339 7587 7339
Size: 248 9657
BbsI GAAGACnn'nnnn_
Cuts at: 125 604 863 2095 3469 3980 125
Size: 479 259 1232 1374 511 6050
Fragments arranged by size:
6050 1374 1232 511 479 259
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 5522 5556 5522
Size: 34 9871
BciVI GTATCCnnnnn_n'
Cuts at: 5897 7803 8859 9905 5897
Size: 1906 1056 1046 5897
Fragments arranged by size:
5897 1906 1056 1046
BclI T'GATC_A
Cuts at: 945 1122 1218 7425 945
Size: 177 96 6207 3425
Fragments arranged by size:
6207 3425 177 96
BfrBI ATG'CAT
Cuts at: 3289 3289
Size: 9905
BglII A'GATC_T
Cuts at: 1336 2463 5154 7420 9457 1336
Size: 1127 2691 2266 2037 1784
Fragments arranged by size:
2691 2266 2037 1784 1127
BlpI GC'TnA_GC
Cuts at: 7017 7017
Size: 9905
Bme1580I G_kGCm'C
Cuts at: 2782 2909 4629 7530 7623 8747 2782
Size: 127 1720 2901 93 1124 3940
Fragments arranged by size:
3940 2901 1720 1124 127 93
BmrI ACTGGGnnnn_n'
Cuts at: 2271 2908 3224 5877 7117 7198 7533 9677 2271
Size: 637 316 2653 1240 81 335 2144 2499
Fragments arranged by size:
2653 2499 2144 1240 637 335 316 81
BmtI G_CTAG'C
Cuts at: 4759 4759
Size: 9905
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 950 8272 950
Size: 7322 2583
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1295 1994 4280 4505 6498 1295
Size: 699 2286 225 1993 4702
Fragments arranged by size:
4702 2286 1993 699 225
Bpu10I CC'TnA_GC
Cuts at: 1459 2804 1459
Size: 1345 8560
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2942 3880 4063 5056 6547 7081 7960 8674 8972
Size: 938 183 993 1491 534 879 714 298
Cuts at: 8972 2942
Size: 3875
Fragments arranged by size:
3875 1491 993 938 879 714 534 298
183
BsaI GGTCTCn'nnnn_
Cuts at: 4807 6226 9609 4807
Size: 1419 3383 5103
Fragments arranged by size:
5103 3383 1419
BsaAI yAC'GTr
Cuts at: 1148 6089 7258 1148
Size: 4941 1169 3795
Fragments arranged by size:
4941 3795 1169
BsaBI GATnn'nnATC
Cuts at: 6 318 7419 9456 6
Size: 312 7101 2037 455
Fragments arranged by size:
7101 2037 455 312
BsaHI Gr'CG_yC
Cuts at: 860 3388 7336 7584 8286 9782 860
Size: 2528 3948 248 702 1496 983
Fragments arranged by size:
3948 2528 1496 983 702 248
BsaWI w'CCGG_w
Cuts at: 2208 7094 7615 8293 8704 8851 9125 2208
Size: 4886 521 678 411 147 274 2988
Fragments arranged by size:
4886 2988 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1874 1904 4323 4353 1874
Size: 30 2419 30 7426
Fragments arranged by size:
7426 2419 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1220 1502 3626 5014 5539 6469 6544 1220
Size: 282 2124 1388 525 930 75 4581
Fragments arranged by size:
4581 2124 1388 930 525 282 75
BseYI C'CCAG_C
Cuts at: 7330 8088 8753 9161 7330
Size: 758 665 408 8074
Fragments arranged by size:
8074 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2729 5797 6735 2729
Size: 3068 938 5899
Fragments arranged by size:
5899 3068 938
BsiEI CG_ry'CG
Cuts at: 104 5542 7447 7493 8723 104
Size: 5438 1905 46 1230 1286
Fragments arranged by size:
5438 1905 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1460 3487 6484 6919 7697 7887 8747 1460
Size: 2027 2997 435 778 190 860 2618
Fragments arranged by size:
2997 2618 2027 860 778 435 190
BsmI GAATG_Cn'
Cuts at: 3224 5769 3224
Size: 2545 7360
Bsp1286I G_dGCh'C
Cuts at: 1460 2782 2909 3487 4629 6484 6919 7530 7623
Size: 1322 127 578 1142 1855 435 611 93
Cuts at: 7623 7697 7887 7949 8747 1460
Size: 74 190 62 798 2618
Fragments arranged by size:
2618 1855 1322 1142 798 611 578 435
190 127 93 74 62
BspHI T'CATG_A
Cuts at: 1125 5319 8337 1125
Size: 4194 3018 2693
Fragments arranged by size:
4194 3018 2693
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2535 2855 5169 6955 7169 7471 7852 2535
Size: 320 2314 1786 214 302 381 4588
Fragments arranged by size:
4588 2314 1786 381 320 302 214
BsrBI CCG'CTC
Cuts at: 50 8197 8251 9735 9900 50
Size: 8147 54 1484 165 55
Fragments arranged by size:
8147 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 6111 7817 6111
Size: 1706 8199
BsrFI r'CCGG_y
Cuts at: 2208 4722 7903 8084 2208
Size: 2514 3181 181 4029
Fragments arranged by size:
4029 3181 2514 181
BsrGI T'GTAC_A
Cuts at: 4591 5142 5863 6684 4591
Size: 551 721 821 7812
Fragments arranged by size:
7812 821 721 551
BssSI C'ACGA_G
Cuts at: 3542 8176 8884 3542
Size: 4634 708 4563
Fragments arranged by size:
4634 4563 708
BstAPI GCAn_nnn'nTGC
Cuts at: 7282 7282
Size: 9905
BstBI TT'CG_AA
Cuts at: 8266 8266
Size: 9905
BstEII G'GTnAC_C
Cuts at: 1771 1771
Size: 9905
BstXI CCAn_nnnn'nTGG
Cuts at: 2898 5458 2898
Size: 2560 7345
BstYI r'GATC_y
Cuts at: 1 1336 2463 2508 3078 3577 4057 5154 6595
Size: 1335 1127 45 570 499 480 1097 1441
Cuts at: 6595 6930 7397 7420 7755 8001 8307 8319 8405
Size: 335 467 23 335 246 306 12 86
Cuts at: 8405 9457 1
Size: 1052 449
Fragments arranged by size:
1441 1335 1127 1097 1052 570 499 480
467 449 335 335 306 246 86 45
23 12
BstZ17I GTA'TAC
Cuts at: 988 5496 988
Size: 4508 5397
BtgI C'CryG_G
Cuts at: 107 2769 5451 107
Size: 2662 2682 4561
Fragments arranged by size:
4561 2682 2662
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 7855 8036 8199 7855
Size: 181 163 9561
Fragments arranged by size:
9561 181 163
BtsI GCAGTG_nn'
Cuts at: 496 1620 4847 6545 6723 7148 496
Size: 1124 3227 1698 178 425 3253
Fragments arranged by size:
3253 3227 1698 1124 425 178
ClaI AT'CG_AT
Cuts at: 5073 5073
Size: 9905
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3320 3355 3320
Size: 35 9870
DraI TTT'AAA
Cuts at: 816 2121 3075 3590 4572 6904 816
Size: 1305 954 515 982 2332 3817
Fragments arranged by size:
3817 2332 1305 982 954 515
DraIII CAC_nnn'GTG
Cuts at: 2902 2902
Size: 9905
DrdI GACnn_nn'nnGTC
Cuts at: 7611 8955 7611
Size: 1344 8561
EaeI y'GGCC_r
Cuts at: 7490 7664 8055 8082 9427 7490
Size: 174 391 27 1345 7968
Fragments arranged by size:
7968 1345 391 174 27
EagI C'GGCC_G
Cuts at: 7490 7490
Size: 9905
EarI CTCTTCn'nnn_
Cuts at: 185 277 2557 2996 5177 6316 7928 8138 185
Size: 92 2280 439 2181 1139 1612 210 1952
Fragments arranged by size:
2280 2181 1952 1612 1139 439 210 92
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 8113 8855 9001 9715 8113
Size: 742 146 714 8303
Fragments arranged by size:
8303 742 714 146
EcoICRI GAG'CTC
Cuts at: 1458 6482 6917 1458
Size: 5024 435 4446
Fragments arranged by size:
5024 4446 435
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1274 1295 1316 1334 1994 2703 3481 3929 3992
Size: 21 21 18 660 709 778 448 63
Cuts at: 3992 4103 4235 4280 4505 4679 4904 5342 5354
Size: 111 132 45 225 174 225 438 12
Cuts at: 5354 5897 6498 6538 6694 6769 7730 8162 8515
Size: 543 601 40 156 75 961 432 353
Cuts at: 8515 9177 1274
Size: 662 2002
Fragments arranged by size:
2002 961 778 709 662 660 601 543
448 438 432 353 225 225 174 156
132 111 75 63 45 40 21 21
18 12
EcoO109I rG'GnC_Cy
Cuts at: 2778 7044 2778
Size: 4266 5639
EcoRI G'AATT_C
Cuts at: 379 1737 3930 4029 6909 379
Size: 1358 2193 99 2880 3375
Fragments arranged by size:
3375 2880 2193 1358 99
EcoRV GAT'ATC
Cuts at: 4368 6864 4368
Size: 2496 7409
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 400 1279 1412 7924 400
Size: 879 133 6512 2381
Fragments arranged by size:
6512 2381 879 133
FspI TGC'GCA
Cuts at: 7686 7686
Size: 9905
HaeII r_GCGC'y
Cuts at: 870 7339 7587 8817 9588 870
Size: 6469 248 1230 771 1187
Fragments arranged by size:
6469 1230 1187 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2488 2520 3303 3335 4323 4355 5386 5418 5839
Size: 32 783 32 988 32 1031 32 421
Cuts at: 5839 5871 6466 6498 7747 7777 7779 7809 2488
Size: 32 595 32 1249 30 2 30 4584
Fragments arranged by size:
4584 1249 1031 988 783 595 421 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2330 3810 6944 2330
Size: 1480 3134 5291
Fragments arranged by size:
5291 3134 1480
HindIII A'AGCT_T
Cuts at: 1106 1106
Size: 9905
HpaI GTT'AAC
Cuts at: 3810 3810
Size: 9905
KasI G'GCGC_C
Cuts at: 7335 7583 7335
Size: 248 9657
KpnI G_GTAC'C
Cuts at: 6925 6925
Size: 9905
MfeI C'AATT_G
Cuts at: 4552 4552
Size: 9905
MluI A'CGCG_T
Cuts at: 9338 9338
Size: 9905
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1988 3346 3466 3778 5068 5577 8664 8848 1988
Size: 1358 120 312 1290 509 3087 184 3045
Fragments arranged by size:
3087 3045 1358 1290 509 312 184 120
MscI TGG'CCA
Cuts at: 7666 7666
Size: 9905
MslI CAynn'nnrTG
Cuts at: 941 1214 1549 3743 4305 4832 6833 7894 8021
Size: 273 335 2194 562 527 2001 1061 127
Cuts at: 8021 941
Size: 2825
Fragments arranged by size:
2825 2194 2001 1061 562 527 335 273
127
MspA1I CmG'CkG
Cuts at: 1423 2027 6397 7016 7330 7690 8474 8719 1423
Size: 604 4370 619 314 360 784 245 2609
Fragments arranged by size:
4370 2609 784 619 604 360 314 245
NaeI GCC'GGC
Cuts at: 4724 8086 4724
Size: 3362 6543
NarI GG'CG_CC
Cuts at: 7336 7584 7336
Size: 248 9657
NcoI C'CATG_G
Cuts at: 107 107
Size: 9905
NdeI CA'TA_TG
Cuts at: 448 448
Size: 9905
NgoMIV G'CCGG_C
Cuts at: 4722 8084 4722
Size: 3362 6543
NheI G'CTAG_C
Cuts at: 4755 4755
Size: 9905
NsiI A_TGCA'T
Cuts at: 3291 3291
Size: 9905
NspI r_CATG'y
Cuts at: 354 409 505 2290 3071 4592 5824 6958 7458
Size: 55 96 1785 781 1521 1232 1134 500
Cuts at: 7458 9061 354
Size: 1603 1198
Fragments arranged by size:
1785 1603 1521 1232 1198 1134 781 500
96 55
PciI A'CATG_T
Cuts at: 4588 9057 4588
Size: 4469 5436
PflMI CCAn_nnn'nTGG
Cuts at: 2179 2941 3669 3921 2179
Size: 762 728 252 8163
Fragments arranged by size:
8163 762 728 252
PfoI T'CCnGG_A
Cuts at: 1688 1688
Size: 9905
PmeI GTTT'AAAC
Cuts at: 6904 6904
Size: 9905
PmlI CAC'GTG
Cuts at: 6089 6089
Size: 9905
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8351 8383 8351
Size: 32 9873
PshAI GACnn'nnGTC
Cuts at: 1479 1479
Size: 9905
PsiI TTA'TAA
Cuts at: 1142 1913 3556 4103 4177 5951 1142
Size: 771 1643 547 74 1774 5096
Fragments arranged by size:
5096 1774 1643 771 547 74
PspOMI G'GGCC_C
Cuts at: 2778 2778
Size: 9905
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2397 2429 6111 6143 2397
Size: 32 3682 32 6159
Fragments arranged by size:
6159 3682 32 32
PstI C_TGCA'G
Cuts at: 2532 4365 6540 6952 7637 2532
Size: 1833 2175 412 685 4800
Fragments arranged by size:
4800 2175 1833 685 412
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9905
PvuII CAG'CTG
Cuts at: 1423 2027 6397 7330 7690 1423
Size: 604 4370 933 360 3638
Fragments arranged by size:
4370 3638 933 604 360
RsrII CG'GwC_CG
Cuts at: 8100 8100
Size: 9905
SacI G_AGCT'C
Cuts at: 1460 6484 6919 1460
Size: 5024 435 4446
Fragments arranged by size:
5024 4446 435
SalI G'TCGA_C
Cuts at: 6942 6942
Size: 9905
SapI GCTCTTCn'nnn_
Cuts at: 7928 8138 7928
Size: 210 9695
SbfI CC_TGCA'GG
Cuts at: 6952 6952
Size: 9905
ScaI AGT'ACT
Cuts at: 811 907 1227 2439 9422 811
Size: 96 320 1212 6983 1294
Fragments arranged by size:
6983 1294 1212 320 96
SfcI C'TryA_G
Cuts at: 33 909 2528 2859 2968 3091 4361 4461 6128
Size: 876 1619 331 109 123 1270 100 1667
Cuts at: 6128 6536 6948 7633 8601 8792 33
Size: 408 412 685 968 191 1146
Fragments arranged by size:
1667 1619 1270 1146 968 876 685 412
408 331 191 123 109 100
SfoI GGC'GCC
Cuts at: 7337 7585 7337
Size: 248 9657
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9905
SgrAI Cr'CCGG_yG
Cuts at: 2208 2208
Size: 9905
SmaI CCC'GGG
Cuts at: 6927 6927
Size: 9905
SmlI C'TyrA_G
Cuts at: 1924 2957 3859 4042 5035 6526 7060 7975 8689
Size: 1033 902 183 993 1491 534 915 714
Cuts at: 8689 8951 1924
Size: 262 2878
Fragments arranged by size:
2878 1491 1033 993 915 902 714 534
262 183
SnaBI TAC'GTA
Cuts at: 1148 1148
Size: 9905
SpeI A'CTAG_T
Cuts at: 67 6640 67
Size: 6573 3332
SphI G_CATG'C
Cuts at: 6958 7458 6958
Size: 500 9405
SspI AAT'ATT
Cuts at: 1323 2493 6621 1323
Size: 1170 4128 4607
Fragments arranged by size:
4607 4128 1170
StuI AGG'CCT
Cuts at: 564 4689 564
Size: 4125 5780
StyI C'CwwG_G
Cuts at: 107 384 4449 7039 7393 107
Size: 277 4065 2590 354 2619
Fragments arranged by size:
4065 2619 2590 354 277
SwaI ATTT'AAAT
Cuts at: 3590 3590
Size: 9905
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2313 2890 7488 7621 8080 8289 2313
Size: 577 4598 133 459 209 3929
Fragments arranged by size:
4598 3929 577 459 209 133
TatI w'GTAC_w
Cuts at: 809 905 1225 2044 2437 3232 4311 4591 4735
Size: 96 320 819 393 795 1079 280 144
Cuts at: 4735 5142 5863 5908 6684 6825 9420 809
Size: 407 721 45 776 141 2595 1294
Fragments arranged by size:
2595 1294 1079 819 795 776 721 407
393 320 280 144 141 96 45
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 9422 9454 9422
Size: 32 9873
Tth111I GACn'n_nGTC
Cuts at: 7702 7702
Size: 9905
XbaI T'CTAG_A
Cuts at: 5439 6936 5439
Size: 1497 8408
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5287 6577 7400 5287
Size: 1290 823 7792
Fragments arranged by size:
7792 1290 823
XmaI C'CCGG_G
Cuts at: 6925 6925
Size: 9905
XmnI GAAnn'nnTTC
Cuts at: 737 1098 4529 737
Size: 361 3431 6113
Fragments arranged by size:
6113 3431 361
ZraI GAC'GTC
Cuts at: 3389 3389
Size: 9905
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NsiI NspI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AhdI AleI AscI AvrII BbvCI BglI BmgBI BsiWI
BsmBI BspEI BssHII Bsu36I EcoNI FseI FspAI NotI
NruI PacI PasI PpuMI PspXI SacII SanDI SexAI
SfiI SrfI XhoI