Restriction map for
pF1KA0536
(Circular) (Six-base) MAPSORT of: pF1KA0536.seq Check: 3506 from: 1 to: 6134
pF1KA0536 6134 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 1374 1581 3172 1374
Size: 207 1591 4336
Fragments arranged by size:
4336 1591 207
Acc65I G'GTAC_C
Cuts at: 3150 3150
Size: 6134
AclI AA'CG_TT
Cuts at: 2163 5974 2163
Size: 3811 2323
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 560 1655 1796 2352 2821 3026 3959 4391 5138
Size: 1095 141 556 469 205 933 432 747
Cuts at: 5138 5406 560
Size: 268 1288
Fragments arranged by size:
1288 1095 933 747 556 469 432 268
205 141
AflIII A'CryG_T
Cuts at: 1787 2625 3486 4590 5567 1787
Size: 838 861 1104 977 2354
Fragments arranged by size:
2354 1104 977 861 838
AgeI A'CCGG_T
Cuts at: 4522 4522
Size: 6134
AhdI GACnn_n'nnGTC
Cuts at: 1023 1023
Size: 6134
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1446 1478 1446
Size: 32 6102
AlwNI CAG_nnn'CTG
Cuts at: 2014 3057 3439 5006 2014
Size: 1043 382 1567 3142
Fragments arranged by size:
3142 1567 1043 382
ApaLI G'TGCA_C
Cuts at: 4904 4904
Size: 6134
ApoI r'AATT_y
Cuts at: 554 1615 2347 2765 3138 5487 5743 554
Size: 1061 732 418 373 2349 256 945
Fragments arranged by size:
2349 1061 945 732 418 373 256
AseI AT'TA_AT
Cuts at: 20 2954 20
Size: 2934 3200
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6134
AvaI C'yCGr_G
Cuts at: 1355 3154 1355
Size: 1799 4335
BamHI G'GATC_C
Cuts at: 3159 3159
Size: 6134
BanI G'GyrC_C
Cuts at: 1992 2990 3150 3564 3812 3847 1992
Size: 998 160 414 248 35 4279
Fragments arranged by size:
4279 998 414 248 160 35
BanII G_rGCy'C
Cuts at: 512 2279 3148 4178 512
Size: 1767 869 1030 2468
Fragments arranged by size:
2468 1767 1030 869
BbeI G_GCGC'C
Cuts at: 3568 3816 3568
Size: 248 5886
BbsI GAAGACnn'nnnn_
Cuts at: 212 302 434 1146 1688 2044 2458 3014 212
Size: 90 132 712 542 356 414 556 3332
Fragments arranged by size:
3332 712 556 542 414 356 132 90
BciVI GTATCCnnnnn_n'
Cuts at: 4032 4793 6134 4032
Size: 761 1341 4032
Fragments arranged by size:
4032 1341 761
BclI T'GATC_A
Cuts at: 2877 3654 2877
Size: 777 5357
BglII A'GATC_T
Cuts at: 595 874 1231 1438 1504 3649 5686 595
Size: 279 357 207 66 2145 2037 1043
Fragments arranged by size:
2145 2037 1043 357 279 207 66
BlpI GC'TnA_GC
Cuts at: 3246 3246
Size: 6134
Bme1580I G_kGCm'C
Cuts at: 3759 3852 4908 3759
Size: 93 1056 4985
Fragments arranged by size:
4985 1056 93
BmgBI CAC'GTC
Cuts at: 1378 1378
Size: 6134
BmrI ACTGGGnnnn_n'
Cuts at: 120 985 2195 3346 3427 3762 5906 120
Size: 865 1210 1151 81 335 2144 348
Fragments arranged by size:
2144 1210 1151 865 348 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 552 4501 552
Size: 3949 2185
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 371 1832 1882 371
Size: 1461 50 4623
Fragments arranged by size:
4623 1461 50
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 788 836 3310 4189 4681 4979 788
Size: 48 2474 879 492 298 1943
Fragments arranged by size:
2474 1943 879 492 298 48
BsaI GGTCTCn'nnnn_
Cuts at: 115 1214 1421 1463 1490 1565 5838 115
Size: 1099 207 42 27 75 4273 411
Fragments arranged by size:
4273 1099 411 207 75 42 27
BsaAI yAC'GTr
Cuts at: 1186 3487 1186
Size: 2301 3833
BsaBI GATnn'nnATC
Cuts at: 6 1842 2333 2882 3648 5685 6
Size: 1836 491 549 766 2037 455
Fragments arranged by size:
2037 1836 766 549 491 455
BsaHI Gr'CG_yC
Cuts at: 3565 3813 4515 6011 3565
Size: 248 702 1496 3688
Fragments arranged by size:
3688 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3323 3844 4522 4796 4943 5354 3323
Size: 521 678 274 147 411 4103
Fragments arranged by size:
4103 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 112 142 1251 1281 1446 1476 112
Size: 30 1109 30 165 30 4770
Fragments arranged by size:
4770 1109 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1338 1499 1548 1338
Size: 161 49 5924
Fragments arranged by size:
5924 161 49
BseYI C'CCAG_C
Cuts at: 1132 3069 3559 4317 4894 5390 1132
Size: 1937 490 758 577 496 1876
Fragments arranged by size:
1937 1876 758 577 496 490
BsiEI CG_ry'CG
Cuts at: 104 969 3676 3722 4930 104
Size: 865 2707 46 1208 1308
Fragments arranged by size:
2707 1308 1208 865 46
BsiHKAI G_wGCw'C
Cuts at: 2049 2279 2679 2950 3148 3926 4116 4908 2049
Size: 230 400 271 198 778 190 792 3275
Fragments arranged by size:
3275 792 778 400 271 230 198 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1320 1320
Size: 6134
Bsp1286I G_dGCh'C
Cuts at: 512 2049 2279 2679 2950 3148 3759 3852 3926
Size: 1537 230 400 271 198 611 93 74
Cuts at: 3926 4116 4178 4908 512
Size: 190 62 730 1738
Fragments arranged by size:
1738 1537 730 611 400 271 230 198
190 93 74 62
BspEI T'CCGG_A
Cuts at: 5354 5354
Size: 6134
BspHI T'CATG_A
Cuts at: 558 5310 558
Size: 4752 1382
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1196 1472 3184 3398 3700 4081 1196
Size: 276 1712 214 302 381 3249
Fragments arranged by size:
3249 1712 381 302 276 214
BsrBI CCG'CTC
Cuts at: 50 4426 4480 5964 6129 50
Size: 4376 54 1484 165 55
Fragments arranged by size:
4376 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2816 4046 2816
Size: 1230 4904
BsrFI r'CCGG_y
Cuts at: 4132 4313 4522 4132
Size: 181 209 5744
Fragments arranged by size:
5744 209 181
BsrGI T'GTAC_A
Cuts at: 2897 2897
Size: 6134
BssSI C'ACGA_G
Cuts at: 4405 4763 4405
Size: 358 5776
BstAPI GCAn_nnn'nTGC
Cuts at: 3511 3511
Size: 6134
BstBI TT'CG_AA
Cuts at: 2847 4495 2847
Size: 1648 4486
BstEII G'GTnAC_C
Cuts at: 1127 1127
Size: 6134
BstYI r'GATC_y
Cuts at: 1 595 667 874 985 1231 1438 1504 2485
Size: 594 72 207 111 246 207 66 981
Cuts at: 2485 2814 3159 3626 3649 3984 4230 5242 5328
Size: 329 345 467 23 335 246 1012 86
Cuts at: 5328 5340 5686 1
Size: 12 346 449
Fragments arranged by size:
1012 981 594 467 449 346 345 335
329 246 246 207 207 111 86 72
66 23 12
BtgI C'CryG_G
Cuts at: 107 116 107
Size: 9 6125
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4084 4265 4428 4084
Size: 181 163 5790
Fragments arranged by size:
5790 181 163
BtsI GCAGTG_nn'
Cuts at: 3377 3377
Size: 6134
DraI TTT'AAA
Cuts at: 1620 2297 2597 3133 1620
Size: 677 300 536 4621
Fragments arranged by size:
4621 677 536 300
DraIII CAC_nnn'GTG
Cuts at: 1573 1573
Size: 6134
DrdI GACnn_nn'nnGTC
Cuts at: 3840 4698 3840
Size: 858 5276
EaeI y'GGCC_r
Cuts at: 110 3719 3893 4284 4311 5656 110
Size: 3609 174 391 27 1345 588
Fragments arranged by size:
3609 1345 588 391 174 27
EagI C'GGCC_G
Cuts at: 3719 3719
Size: 6134
EarI CTCTTCn'nnn_
Cuts at: 539 1272 2371 4157 4367 539
Size: 733 1099 1786 210 2306
Fragments arranged by size:
2306 1786 1099 733 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4342 4652 4798 5944 4342
Size: 310 146 1146 4532
Fragments arranged by size:
4532 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2277 3146 2277
Size: 869 5265
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 371 560 1655 1796 1832 1882 2352 2821 3026
Size: 189 1095 141 36 50 470 469 205
Cuts at: 3026 3959 4391 5138 5406 371
Size: 933 432 747 268 1099
Fragments arranged by size:
1099 1095 933 747 470 469 432 268
205 189 141 50 36
EcoO109I rG'GnC_Cy
Cuts at: 2967 3096 3273 2967
Size: 129 177 5828
Fragments arranged by size:
5828 177 129
EcoRI G'AATT_C
Cuts at: 3138 3138
Size: 6134
EcoRV GAT'ATC
Cuts at: 1365 2553 1365
Size: 1188 4946
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1645 4153 1645
Size: 2508 3626
FspI TGC'GCA
Cuts at: 3915 3915
Size: 6134
HaeII r_GCGC'y
Cuts at: 3568 3816 4838 5817 3568
Size: 248 1022 979 3885
Fragments arranged by size:
3885 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 112 144 367 399 1009 1030 1041 1062 1093
Size: 32 223 32 610 21 11 21 31
Cuts at: 1093 1125 1216 1248 1251 1283 1349 1381 1642
Size: 32 91 32 3 32 66 32 261
Cuts at: 1642 1674 2522 2554 2599 2631 2710 2742 3976
Size: 32 848 32 45 32 79 32 1234
Cuts at: 3976 4006 4008 4038 112
Size: 30 2 30 2208
Fragments arranged by size:
2208 1234 848 610 261 223 91 79
66 45 32 32 32 32 32 32
32 32 32 32 31 30 30 21
21 11 3 2
HincII GTy'rAC
Cuts at: 3173 3173
Size: 6134
HindIII A'AGCT_T
Cuts at: 2599 2803 2599
Size: 204 5930
KasI G'GCGC_C
Cuts at: 3564 3812 3564
Size: 248 5886
KpnI G_GTAC'C
Cuts at: 3154 3154
Size: 6134
MfeI C'AATT_G
Cuts at: 1748 1748
Size: 6134
MluI A'CGCG_T
Cuts at: 5567 5567
Size: 6134
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1444 1510 2983 3026 3044 4805 4989 1444
Size: 66 1473 43 18 1761 184 2589
Fragments arranged by size:
2589 1761 1473 184 66 43 18
MscI TGG'CCA
Cuts at: 3895 3895
Size: 6134
MslI CAynn'nnrTG
Cuts at: 1792 4123 4250 1792
Size: 2331 127 3676
Fragments arranged by size:
3676 2331 127
MspA1I CmG'CkG
Cuts at: 118 2062 3245 3559 3919 4932 5177 118
Size: 1944 1183 314 360 1013 245 1075
Fragments arranged by size:
1944 1183 1075 1013 360 314 245
NaeI GCC'GGC
Cuts at: 4315 4315
Size: 6134
NarI GG'CG_CC
Cuts at: 3565 3813 3565
Size: 248 5886
NcoI C'CATG_G
Cuts at: 107 107
Size: 6134
NdeI CA'TA_TG
Cuts at: 2795 2795
Size: 6134
NgoMIV G'CCGG_C
Cuts at: 4313 4313
Size: 6134
NruI TCG'CGA
Cuts at: 965 965
Size: 6134
NspI r_CATG'y
Cuts at: 1791 2548 2629 3187 3687 4594 1791
Size: 757 81 558 500 907 3331
Fragments arranged by size:
3331 907 757 558 500 81
PciI A'CATG_T
Cuts at: 1787 2625 4590 1787
Size: 838 1965 3331
Fragments arranged by size:
3331 1965 838
PflMI CCAn_nnn'nTGG
Cuts at: 2247 2247
Size: 6134
PfoI T'CCnGG_A
Cuts at: 3116 3116
Size: 6134
PmeI GTTT'AAAC
Cuts at: 3133 3133
Size: 6134
PmlI CAC'GTG
Cuts at: 1186 1186
Size: 6134
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1338 1370 1446 1478 5273 5305 1338
Size: 32 76 32 3795 32 2167
Fragments arranged by size:
3795 2167 76 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2967 2967
Size: 6134
PshAI GACnn'nnGTC
Cuts at: 690 1526 690
Size: 836 5298
PsiI TTA'TAA
Cuts at: 946 946
Size: 6134
PstI C_TGCA'G
Cuts at: 3181 3866 3181
Size: 685 5449
PvuI CG_AT'CG
Cuts at: 104 969 104
Size: 865 5269
PvuII CAG'CTG
Cuts at: 3559 3919 3559
Size: 360 5774
RsrII CG'GwC_CG
Cuts at: 4329 4329
Size: 6134
SacI G_AGCT'C
Cuts at: 2279 3148 2279
Size: 869 5265
SacII CC_GC'GG
Cuts at: 119 119
Size: 6134
SalI G'TCGA_C
Cuts at: 3171 3171
Size: 6134
SapI GCTCTTCn'nnn_
Cuts at: 1272 4157 4367 1272
Size: 2885 210 3039
Fragments arranged by size:
3039 2885 210
SbfI CC_TGCA'GG
Cuts at: 3181 3181
Size: 6134
ScaI AGT'ACT
Cuts at: 591 5651 591
Size: 5060 1074
SexAI A'CCwGG_T
Cuts at: 1143 1143
Size: 6134
SfcI C'TryA_G
Cuts at: 33 2490 2729 3100 3177 3862 4855 5046 33
Size: 2457 239 371 77 685 993 191 1121
Fragments arranged by size:
2457 1121 993 685 371 239 191 77
SfoI GGC'GCC
Cuts at: 3566 3814 3566
Size: 248 5886
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6134
SmaI CCC'GGG
Cuts at: 3156 3156
Size: 6134
SmlI C'TyrA_G
Cuts at: 803 851 1355 3289 4204 4696 4958 803
Size: 48 504 1934 915 492 262 1979
Fragments arranged by size:
1979 1934 915 504 492 262 48
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6134
SphI G_CATG'C
Cuts at: 3187 3687 3187
Size: 500 5634
StuI AGG'CCT
Cuts at: 1921 1921
Size: 6134
StyI C'CwwG_G
Cuts at: 107 3268 3622 107
Size: 3161 354 2619
Fragments arranged by size:
3161 2619 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1146 2128 2978 3717 3850 4309 4518 1146
Size: 982 850 739 133 459 209 2762
Fragments arranged by size:
2762 982 850 739 459 209 133
TatI w'GTAC_w
Cuts at: 589 604 2897 3024 5649 589
Size: 15 2293 127 2625 1074
Fragments arranged by size:
2625 2293 1074 127 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2940 2972 5651 5683 2940
Size: 32 2679 32 3391
Fragments arranged by size:
3391 2679 32 32
Tth111I GACn'n_nGTC
Cuts at: 126 1145 1263 3931 126
Size: 1019 118 2668 2329
Fragments arranged by size:
2668 2329 1019 118
XbaI T'CTAG_A
Cuts at: 3165 3165
Size: 6134
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2244 3629 2244
Size: 1385 4749
XhoI C'TCGA_G
Cuts at: 1355 1355
Size: 6134
XmaI C'CCGG_G
Cuts at: 3154 3154
Size: 6134
XmnI GAAnn'nnTTC
Cuts at: 1629 1629
Size: 6134
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NruI
NspI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmtI Bpu10I
BsgI BsiWI BsmI BssHII BstXI BstZ17I Bsu36I ClaI
CspCI EcoNI FseI FspAI HpaI NheI NotI NsiI
PacI PasI PspOMI PspXI PsrI SanDI SfiI SgrAI
SnaBI SrfI SspI SwaI ZraI