Restriction map for
pF1KA0540
(Circular) (Six-base) MAPSORT of: pF1KA0540.seq Check: 6678 from: 1 to: 11375
pF1KA0540 11375 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 250 1346 1721 1771 3114 4089 6047 250
Size: 1096 375 50 1343 975 1958 5578
Fragments arranged by size:
5578 1958 1343 1096 975 375 50
AccI GT'mk_AC
Cuts at: 2201 5945 7034 8413 2201
Size: 3744 1089 1379 5163
Fragments arranged by size:
5163 3744 1379 1089
Acc65I G'GTAC_C
Cuts at: 1881 8391 1881
Size: 6510 4865
AclI AA'CG_TT
Cuts at: 11215 11215
Size: 11375
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 2163 2855 2912 2974 4076 4736 5494 5647
Size: 1955 692 57 62 1102 660 758 153
Cuts at: 5647 7141 7389 7395 9200 9632 10379 10647 208
Size: 1494 248 6 1805 432 747 268 936
Fragments arranged by size:
1955 1805 1494 1102 936 758 747 692
660 432 268 248 153 62 57 6
AfeI AGC'GCT
Cuts at: 4676 5086 5673 4676
Size: 410 587 10378
Fragments arranged by size:
10378 587 410
AflIII A'CryG_T
Cuts at: 3239 5291 6425 8727 9831 10808 3239
Size: 2052 1134 2302 1104 977 3806
Fragments arranged by size:
3806 2302 2052 1134 1104 977
AgeI A'CCGG_T
Cuts at: 9763 9763
Size: 11375
AhdI GACnn_n'nnGTC
Cuts at: 2885 5316 6426 7109 2885
Size: 2431 1110 683 7151
Fragments arranged by size:
7151 2431 1110 683
AleI CACnn'nnGTG
Cuts at: 1573 1781 4450 4561 5467 1573
Size: 208 2669 111 906 7481
Fragments arranged by size:
7481 2669 906 208 111
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2656 3670 5103 7784 8227 2656
Size: 1014 1433 2681 443 5804
Fragments arranged by size:
5804 2681 1433 1014 443
AlwNI CAG_nnn'CTG
Cuts at: 186 1482 1656 1786 1909 2190 2904 2959 3114
Size: 1296 174 130 123 281 714 55 155
Cuts at: 3114 3273 3735 4737 5032 5068 5077 5412 6906
Size: 159 462 1002 295 36 9 335 1494
Cuts at: 6906 7119 7212 7452 7752 8680 10247 186
Size: 213 93 240 300 928 1567 1314
Fragments arranged by size:
1567 1494 1314 1296 1002 928 714 462
335 300 295 281 240 213 174 159
155 130 123 93 55 36 9
ApaI G_GGCC'C
Cuts at: 2585 3054 3149 7983 2585
Size: 469 95 4834 5977
Fragments arranged by size:
5977 4834 469 95
ApaLI G'TGCA_C
Cuts at: 2076 2571 10145 2076
Size: 495 7574 3306
Fragments arranged by size:
7574 3306 495
ApoI r'AATT_y
Cuts at: 643 6760 8379 10728 10984 643
Size: 6117 1619 2349 256 1034
Fragments arranged by size:
6117 2349 1619 1034 256
AscI GG'CGCG_CC
Cuts at: 5440 5440
Size: 11375
AseI AT'TA_AT
Cuts at: 20 20
Size: 11375
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 11375
AvaI C'yCGr_G
Cuts at: 422 1154 1165 5770 7670 8334 8395 422
Size: 732 11 4605 1900 664 61 3402
Fragments arranged by size:
4605 3402 1900 732 664 61 11
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3835 3868 3835
Size: 33 11342
BamHI G'GATC_C
Cuts at: 8400 8400
Size: 11375
BanI G'GyrC_C
Cuts at: 202 566 1158 1817 1881 2168 2467 2595 2717
Size: 364 592 659 64 287 299 128 122
Cuts at: 2717 2785 5100 5237 5369 5528 5634 6008 6577
Size: 68 2315 137 132 159 106 374 569
Cuts at: 6577 7360 7470 7731 7891 8095 8391 8805 9053
Size: 783 110 261 160 204 296 414 248
Cuts at: 9053 9088 202
Size: 35 2489
Fragments arranged by size:
2489 2315 783 659 592 569 414 374
364 299 296 287 261 248 204 160
159 137 132 128 122 110 106 68
64 35
BanII G_rGCy'C
Cuts at: 988 1208 2585 2630 3054 3149 4370 6077 6632
Size: 220 1377 45 424 95 1221 1707 555
Cuts at: 6632 6733 6752 7960 7983 8389 9419 988
Size: 101 19 1208 23 406 1030 2944
Fragments arranged by size:
2944 1707 1377 1221 1208 1030 555 424
406 220 101 95 45 23 19
BbeI G_GCGC'C
Cuts at: 570 1162 2172 2721 5241 5373 5532 6012 8809
Size: 592 1010 549 2520 132 159 480 2797
Cuts at: 8809 9057 570
Size: 248 2888
Fragments arranged by size:
2888 2797 2520 1010 592 549 480 248
159 132
BbsI GAAGACnn'nnnn_
Cuts at: 1067 4013 4092 4886 5210 6453 6996 1067
Size: 2946 79 794 324 1243 543 5446
Fragments arranged by size:
5446 2946 1243 794 543 324 79
BbvCI CC'TCA_GC
Cuts at: 1090 1757 3134 3868 4551 5563 6445 7151 1090
Size: 667 1377 734 683 1012 882 706 5314
Fragments arranged by size:
5314 1377 1012 882 734 706 683 667
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4480 4514 8223 8257 8268 8302 4480
Size: 34 3709 34 11 34 7553
Fragments arranged by size:
7553 3709 34 34 34 11
BciVI GTATCCnnnnn_n'
Cuts at: 1944 2284 9273 10034 11375 1944
Size: 340 6989 761 1341 1944
Fragments arranged by size:
6989 1944 1341 761 340
BclI T'GATC_A
Cuts at: 7838 8895 7838
Size: 1057 10318
BglI GCCn_nnn'nGGC
Cuts at: 246 1592 1633 2347 2548 2726 3949 4265 5145
Size: 1346 41 714 201 178 1223 316 880
Cuts at: 5145 6715 6991 246
Size: 1570 276 4630
Fragments arranged by size:
4630 1570 1346 1223 880 714 316 276
201 178 41
BglII A'GATC_T
Cuts at: 3828 8890 10927 3828
Size: 5062 2037 4276
Fragments arranged by size:
5062 4276 2037
BlpI GC'TnA_GC
Cuts at: 1502 2629 3457 4085 5813 7600 7959 8487 1502
Size: 1127 828 628 1728 1787 359 528 4390
Fragments arranged by size:
4390 1787 1728 1127 828 628 528 359
Bme1580I G_kGCm'C
Cuts at: 422 933 1432 2080 2575 2585 2790 3054 3149
Size: 511 499 648 495 10 205 264 95
Cuts at: 3149 5639 6130 7363 7983 8098 9000 9093 10149
Size: 2490 491 1233 620 115 902 93 1056
Cuts at: 10149 422
Size: 1648
Fragments arranged by size:
2490 1648 1233 1056 902 648 620 511
499 495 491 264 205 115 95 93
10
BmgBI CAC'GTC
Cuts at: 3435 4469 6648 3435
Size: 1034 2179 8162
Fragments arranged by size:
8162 2179 1034
BmrI ACTGGGnnnn_n'
Cuts at: 1684 2373 2693 5002 5271 5523 6130 7554 8097
Size: 689 320 2309 269 252 607 1424 543
Cuts at: 8097 8587 8668 9003 11147 1684
Size: 490 81 335 2144 1912
Fragments arranged by size:
2309 2144 1912 1424 689 607 543 490
335 320 269 252 81
BmtI G_CTAG'C
Cuts at: 1627 7273 1627
Size: 5646 5729
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2280 5106 5588 5739 9742 2280
Size: 2826 482 151 4003 3913
Fragments arranged by size:
4003 3913 2826 482 151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 352 403 761 834 1651 1969 2524 2824
Size: 96 51 358 73 817 318 555 300
Cuts at: 2824 3712 3841 4009 4235 4311 4366 4618 4630
Size: 888 129 168 226 76 55 252 12
Cuts at: 4630 5002 5467 5839 5944 6745 6802 6805 6928
Size: 372 465 372 105 801 57 3 123
Cuts at: 6928 7096 8245 256
Size: 168 1149 3386
Fragments arranged by size:
3386 1149 888 817 801 555 465 372
372 358 318 300 252 226 168 168
129 123 105 96 76 73 57 55
51 12 3
Bpu10I CC'TnA_GC
Cuts at: 987 1090 1757 2025 3134 3686 3868 4551 5563
Size: 103 667 268 1109 552 182 683 1012
Cuts at: 5563 6256 6445 7151 987
Size: 693 189 706 5211
Fragments arranged by size:
5211 1109 1012 706 693 683 667 552
268 189 182 103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 958 6091 6230 7207 8551 9430 9922 10220
Size: 621 5133 139 977 1344 879 492 298
Cuts at: 10220 337
Size: 1492
Fragments arranged by size:
5133 1492 1344 977 879 621 492 298
139
BsaI GGTCTCn'nnnn_
Cuts at: 2734 3730 5815 6156 11079 2734
Size: 996 2085 341 4923 3030
Fragments arranged by size:
4923 3030 2085 996 341
BsaAI yAC'GTr
Cuts at: 1834 4908 5294 6426 7682 8211 8728 1834
Size: 3074 386 1132 1256 529 517 4481
Fragments arranged by size:
4481 3074 1256 1132 529 517 386
BsaBI GATnn'nnATC
Cuts at: 6 8889 10926 6
Size: 8883 2037 455
Fragments arranged by size:
8883 2037 455
BsaHI Gr'CG_yC
Cuts at: 567 1034 1159 1241 2169 2718 3290 5238 5370
Size: 467 125 82 928 549 572 1948 132
Cuts at: 5370 5529 6009 8806 9054 9756 11252 567
Size: 159 480 2797 248 702 1496 690
Fragments arranged by size:
2797 1948 1496 928 702 690 572 549
480 467 248 159 132 125 82
BsaWI w'CCGG_w
Cuts at: 8564 9085 9763 10037 10184 10595 8564
Size: 521 678 274 147 411 9344
Fragments arranged by size:
9344 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 112 142 4233 4263 6901 6907 6931 6937 112
Size: 30 4091 30 2638 6 24 6 4550
Fragments arranged by size:
4550 4091 2638 30 30 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 268 899 2256 2635 4969 5731 5998 7003 268
Size: 631 1357 379 2334 762 267 1005 4640
Fragments arranged by size:
4640 2334 1357 1005 762 631 379 267
BseYI C'CCAG_C
Cuts at: 1861 2388 2408 2514 3074 3107 3770 4538 4542
Size: 527 20 106 560 33 663 768 4
Cuts at: 4542 4667 5142 6020 6167 6453 6509 6546 7356
Size: 125 475 878 147 286 56 37 810
Cuts at: 7356 8091 8800 9558 10135 10631 1861
Size: 735 709 758 577 496 2605
Fragments arranged by size:
2605 878 810 768 758 735 709 663
577 560 527 496 475 286 147 125
106 56 37 33 20 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 284 301 896 1597 1814 1924 2801 3043 3394
Size: 17 595 701 217 110 877 242 351
Cuts at: 3394 3457 3511 3562 4149 4973 5494 6628 6911
Size: 63 54 51 587 824 521 1134 283
Cuts at: 6911 6999 7553 7765 8098 284
Size: 88 554 212 333 3561
Fragments arranged by size:
3561 1134 877 824 701 595 587 554
521 351 333 283 242 217 212 110
88 63 54 51 17
BsiEI CG_ry'CG
Cuts at: 104 2054 3639 5879 6684 8147 8917 8963 10171
Size: 1950 1585 2240 805 1463 770 46 1208
Cuts at: 10171 104
Size: 1308
Fragments arranged by size:
2240 1950 1585 1463 1308 1208 805 770
46
BsiHKAI G_wGCw'C
Cuts at: 988 1839 2080 2575 2630 3098 3263 4535 4973
Size: 851 241 495 55 468 165 1272 438
Cuts at: 4973 5764 6077 6752 7960 8389 9167 9357 10149
Size: 791 313 675 1208 429 778 190 792
Cuts at: 10149 988
Size: 2214
Fragments arranged by size:
2214 1272 1208 851 792 791 778 675
495 468 438 429 313 241 190 165
55
BsmI GAATG_Cn'
Cuts at: 681 5337 7228 681
Size: 4656 1891 4828
Fragments arranged by size:
4828 4656 1891
BsmBI CGTCTCn'nnnn_
Cuts at: 715 2584 8335 715
Size: 1869 5751 3755
Fragments arranged by size:
5751 3755 1869
Bsp1286I G_dGCh'C
Cuts at: 422 933 988 1208 1432 1839 2080 2575 2585
Size: 511 55 220 224 407 241 495 10
Cuts at: 2585 2630 2790 3054 3098 3149 3263 4370 4535
Size: 45 160 264 44 51 114 1107 165
Cuts at: 4535 4973 5639 5764 6077 6130 6632 6733 6752
Size: 438 666 125 313 53 502 101 19
Cuts at: 6752 7363 7960 7983 8098 8389 9000 9093 9167
Size: 611 597 23 115 291 611 93 74
Cuts at: 9167 9357 9419 10149 422
Size: 190 62 730 1648
Fragments arranged by size:
1648 1107 730 666 611 611 597 511
502 495 438 407 313 291 264 241
224 220 190 165 160 125 115 114
101 93 74 62 55 53 51 45
44 23 19 10
BspEI T'CCGG_A
Cuts at: 10595 10595
Size: 11375
BspHI T'CATG_A
Cuts at: 1280 10551 1280
Size: 9271 2104
BspMI ACCTGCnnnn'nnnn_
Cuts at: 182 250 1154 1282 1346 1721 1771 2830 3114
Size: 68 904 128 64 375 50 1059 284
Cuts at: 3114 3429 3518 4089 5433 6047 6062 7633 7737
Size: 315 89 571 1344 614 15 1571 104
Cuts at: 7737 8425 8639 8941 9322 182
Size: 688 214 302 381 2235
Fragments arranged by size:
2235 1571 1344 1059 904 688 614 571
381 375 315 302 284 214 128 104
89 68 64 50 15
BsrBI CCG'CTC
Cuts at: 50 123 2375 3678 3690 6266 7059 9667 9721
Size: 73 2252 1303 12 2576 793 2608 54
Cuts at: 9721 11205 11370 50
Size: 1484 165 55
Fragments arranged by size:
2608 2576 2252 1484 1303 793 165 73
55 54 12
BsrDI GCAATG_nn'
Cuts at: 1244 1489 6359 9287 1244
Size: 245 4870 2928 3332
Fragments arranged by size:
4870 3332 2928 245
BsrFI r'CCGG_y
Cuts at: 1064 1458 2171 4003 6683 7286 9373 9554 9763
Size: 394 713 1832 2680 603 2087 181 209
Cuts at: 9763 1064
Size: 2676
Fragments arranged by size:
2680 2676 2087 1832 713 603 394 209
181
BsrGI T'GTAC_A
Cuts at: 1890 2000 1890
Size: 110 11265
BssHII G'CGCG_C
Cuts at: 5382 5440 8365 5382
Size: 58 2925 8392
Fragments arranged by size:
8392 2925 58
BssSI C'ACGA_G
Cuts at: 911 2863 6216 6514 6937 9646 10004 911
Size: 1952 3353 298 423 2709 358 2282
Fragments arranged by size:
3353 2709 2282 1952 423 358 298
BstAPI GCAn_nnn'nTGC
Cuts at: 1100 1392 1482 2959 3453 3966 4923 5068 5077
Size: 292 90 1477 494 513 957 145 9
Cuts at: 5077 7452 8752 1100
Size: 2375 1300 3723
Fragments arranged by size:
3723 2375 1477 1300 957 513 494 292
145 90 9
BstBI TT'CG_AA
Cuts at: 5282 9736 5282
Size: 4454 6921
BstEII G'GTnAC_C
Cuts at: 169 1414 4726 5928 7986 169
Size: 1245 3312 1202 2058 3558
Fragments arranged by size:
3558 3312 2058 1245 1202
BstXI CCAn_nnnn'nTGG
Cuts at: 450 577 1050 2164 2502 4344 6489 7549 7686
Size: 127 473 1114 338 1842 2145 1060 137
Cuts at: 7686 8169 450
Size: 483 3656
Fragments arranged by size:
3656 2145 1842 1114 1060 483 473 338
137 127
BstYI r'GATC_y
Cuts at: 1 3650 3828 7819 8400 8867 8890 9225 9471
Size: 3649 178 3991 581 467 23 335 246
Cuts at: 9471 10483 10569 10581 10927 1
Size: 1012 86 12 346 449
Fragments arranged by size:
3991 3649 1012 581 467 449 346 335
246 178 86 23 12
BstZ17I GTA'TAC
Cuts at: 5946 5946
Size: 11375
Bsu36I CC'TnA_GG
Cuts at: 1531 2609 2660 3752 6291 6875 1531
Size: 1078 51 1092 2539 584 6031
Fragments arranged by size:
6031 2539 1092 1078 584 51
BtgI C'CryG_G
Cuts at: 107 819 1359 1697 3080 4291 4643 4722 5498
Size: 712 540 338 1383 1211 352 79 776
Cuts at: 5498 5906 6859 7427 7465 7578 7857 107
Size: 408 953 568 38 113 279 3625
Fragments arranged by size:
3625 1383 1211 953 776 712 568 540
408 352 338 279 113 79 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1911 3494 4505 6857 7150 9325 9506 9669 1911
Size: 1583 1011 2352 293 2175 181 163 3617
Fragments arranged by size:
3617 2352 2175 1583 1011 293 181 163
BtsI GCAGTG_nn'
Cuts at: 187 582 1483 1660 2139 2960 3031 3619 4214
Size: 395 901 177 479 821 71 588 595
Cuts at: 4214 4242 4943 5158 5364 5474 5725 5744 7485
Size: 28 701 215 206 110 251 19 1741
Cuts at: 7485 7511 7778 8035 8134 8618 187
Size: 26 267 257 99 484 2944
Fragments arranged by size:
2944 1741 901 821 701 595 588 484
479 395 267 257 251 215 206 177
110 99 71 28 26 19
ClaI AT'CG_AT
Cuts at: 6086 6086
Size: 11375
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2385 2420 5181 5216 5756 5791 2385
Size: 35 2761 35 540 35 7969
Fragments arranged by size:
7969 2761 540 35 35 35
DraI TTT'AAA
Cuts at: 8374 8374
Size: 11375
DraIII CAC_nnn'GTG
Cuts at: 2145 2483 2736 3003 6699 7682 2145
Size: 338 253 267 3696 983 5838
Fragments arranged by size:
5838 3696 983 338 267 253
DrdI GACnn_nn'nnGTC
Cuts at: 3439 9081 9939 3439
Size: 5642 858 4875
Fragments arranged by size:
5642 4875 858
EaeI y'GGCC_r
Cuts at: 110 305 970 1066 1298 1726 1811 1900 2051
Size: 195 665 96 232 428 85 89 151
Cuts at: 2051 2211 2525 3470 3610 4314 4515 4655 4805
Size: 160 314 945 140 704 201 140 150
Cuts at: 4805 5876 5903 6663 7539 7784 8144 8171 8186
Size: 1071 27 760 876 245 360 27 15
Cuts at: 8186 8960 9134 9525 9552 10897 110
Size: 774 174 391 27 1345 588
Fragments arranged by size:
1345 1071 945 876 774 760 704 665
588 428 391 360 314 245 232 201
195 174 160 151 150 140 140 96
89 85 27 27 27 15
EagI C'GGCC_G
Cuts at: 2051 5876 8144 8960 2051
Size: 3825 2268 816 4466
Fragments arranged by size:
4466 3825 2268 816
EarI CTCTTCn'nnn_
Cuts at: 208 364 598 727 992 1519 3184 4421 5019
Size: 156 234 129 265 527 1665 1237 598
Cuts at: 5019 6082 9398 9608 208
Size: 1063 3316 210 1975
Fragments arranged by size:
3316 1975 1665 1237 1063 598 527 265
234 210 156 129
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 469 1766 3093 6384 9583 9893 10039 11185 469
Size: 1297 1327 3291 3199 310 146 1146 659
Fragments arranged by size:
3291 3199 1327 1297 1146 659 310 146
EcoICRI GAG'CTC
Cuts at: 986 2628 6075 6750 7958 8387 986
Size: 1642 3447 675 1208 429 3974
Fragments arranged by size:
3974 3447 1642 1208 675 429
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 256 352 403 761 834 1651 1969 2163
Size: 48 96 51 358 73 817 318 194
Cuts at: 2163 2524 2824 2855 2912 2974 3712 3841 4009
Size: 361 300 31 57 62 738 129 168
Cuts at: 4009 4076 4235 4311 4366 4618 4630 4736 5002
Size: 67 159 76 55 252 12 106 266
Cuts at: 5002 5467 5494 5647 5839 5944 6745 6802 6805
Size: 465 27 153 192 105 801 57 3
Cuts at: 6805 6928 7096 7141 7389 7395 8245 9200 9632
Size: 123 168 45 248 6 850 955 432
Cuts at: 9632 10379 10647 208
Size: 747 268 936
Fragments arranged by size:
955 936 850 817 801 747 738 465
432 361 358 318 300 268 266 252
248 194 192 168 168 159 153 129
123 106 105 96 76 73 67 62
57 57 55 51 48 45 31 27
12 6 3
EcoNI CCTnn'n_nnAGG
Cuts at: 1342 2437 3061 3541 7705 1342
Size: 1095 624 480 4164 5012
Fragments arranged by size:
5012 4164 1095 624 480
EcoO109I rG'GnC_Cy
Cuts at: 162 270 537 789 991 1002 1221 1825 1906
Size: 108 267 252 202 11 219 604 81
Cuts at: 1906 2057 2564 2581 2869 2901 3034 3050 3051
Size: 151 507 17 288 32 133 16 1
Cuts at: 3051 3066 3146 3783 4116 4547 4638 4650 5045
Size: 15 80 637 333 431 91 12 395
Cuts at: 5045 5552 6411 6468 6543 6993 7749 7979 8514
Size: 507 859 57 75 450 756 230 535
Cuts at: 8514 162
Size: 3023
Fragments arranged by size:
3023 859 756 637 604 535 507 507
450 431 395 333 288 267 252 230
219 202 151 133 108 91 81 80
75 57 32 17 16 15 12 11
1
EcoRI G'AATT_C
Cuts at: 643 6760 8379 643
Size: 6117 1619 3639
Fragments arranged by size:
6117 3639 1619
EcoRV GAT'ATC
Cuts at: 6326 6326
Size: 11375
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1021 7248 9394 1021
Size: 6227 2146 3002
Fragments arranged by size:
6227 3002 2146
FspI TGC'GCA
Cuts at: 3316 3658 5113 5429 8275 9156 3316
Size: 342 1455 316 2846 881 5535
Fragments arranged by size:
5535 2846 1455 881 342 316
FspAI rTGC'GCAy
Cuts at: 5113 5113
Size: 11375
HaeII r_GCGC'y
Cuts at: 570 653 1162 2172 2721 3345 3705 4678 5088
Size: 83 509 1010 549 624 360 973 410
Cuts at: 5088 5241 5373 5524 5532 5563 5603 5675 6012
Size: 153 132 151 8 31 40 72 337
Cuts at: 6012 8809 9057 10079 11058 570
Size: 2797 248 1022 979 887
Fragments arranged by size:
2797 1022 1010 979 973 887 624 549
509 410 360 337 248 153 151 132
83 72 40 31 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1279 1311 1347 1379 2871 2903 4233 4265 4375
Size: 32 36 32 1492 32 1330 32 110
Cuts at: 4375 4407 5029 5061 5302 5334 6412 6444 6907
Size: 32 622 32 241 32 1078 32 463
Cuts at: 6907 6939 7095 7127 7174 7206 9217 9247 9249
Size: 32 156 32 47 32 2011 30 2
Cuts at: 9249 9279 1279
Size: 30 3375
Fragments arranged by size:
3375 2011 1492 1330 1078 622 463 241
156 110 47 36 32 32 32 32
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 440 8414 440
Size: 7974 3401
HindIII A'AGCT_T
Cuts at: 6534 6534
Size: 11375
KasI G'GCGC_C
Cuts at: 566 1158 2168 2717 5237 5369 5528 6008 8805
Size: 592 1010 549 2520 132 159 480 2797
Cuts at: 8805 9053 566
Size: 248 2888
Fragments arranged by size:
2888 2797 2520 1010 592 549 480 248
159 132
KpnI G_GTAC'C
Cuts at: 1885 8395 1885
Size: 6510 4865
MfeI C'AATT_G
Cuts at: 1098 1098
Size: 11375
MluI A'CGCG_T
Cuts at: 10808 10808
Size: 11375
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 479 1583 3548 6353 6704 6893 7159 8148 10046
Size: 1104 1965 2805 351 189 266 989 1898
Cuts at: 10046 10230 479
Size: 184 1624
Fragments arranged by size:
2805 1965 1898 1624 1104 989 351 266
189 184
MscI TGG'CCA
Cuts at: 972 1813 1902 2527 4517 4657 4807 7541 7786
Size: 841 89 625 1990 140 150 2734 245
Cuts at: 7786 8173 9136 972
Size: 387 963 3211
Fragments arranged by size:
3211 2734 1990 963 841 625 387 245
150 140 89
MslI CAynn'nnrTG
Cuts at: 1042 1405 1573 1781 3085 3091 4450 4561 5232
Size: 363 168 208 1304 6 1359 111 671
Cuts at: 5232 5350 5467 6487 6622 7547 7613 7684 7709
Size: 118 117 1020 135 925 66 71 25
Cuts at: 7709 7810 8167 8245 9364 9491 1042
Size: 101 357 78 1119 127 2926
Fragments arranged by size:
2926 1359 1304 1119 1020 925 671 363
357 208 168 135 127 118 117 111
101 78 71 66 25 6
MspA1I CmG'CkG
Cuts at: 276 315 976 1501 1550 1640 1653 1998 2021
Size: 39 661 525 49 90 13 345 23
Cuts at: 2021 2287 2848 3111 3133 3143 3336 3849 4293
Size: 266 561 263 22 10 193 513 444
Cuts at: 4293 4542 4819 4890 5059 5068 5077 5142 5367
Size: 249 277 71 169 9 9 65 225
Cuts at: 5367 5585 5889 6024 6654 6822 6972 7116 7150
Size: 218 304 135 630 168 150 144 34
Cuts at: 7150 7370 7535 7604 7859 8486 8800 9160 10173
Size: 220 165 69 255 627 314 360 1013
Cuts at: 10173 10418 276
Size: 245 1233
Fragments arranged by size:
1233 1013 661 630 627 561 525 513
444 360 345 314 304 277 266 263
255 249 245 225 220 218 193 169
168 165 150 144 135 90 71 69
65 49 39 34 23 22 13 10
9 9
NaeI GCC'GGC
Cuts at: 2173 4005 9556 2173
Size: 1832 5551 3992
Fragments arranged by size:
5551 3992 1832
NarI GG'CG_CC
Cuts at: 567 1159 2169 2718 5238 5370 5529 6009 8806
Size: 592 1010 549 2520 132 159 480 2797
Cuts at: 8806 9054 567
Size: 248 2888
Fragments arranged by size:
2888 2797 2520 1010 592 549 480 248
159 132
NcoI C'CATG_G
Cuts at: 107 1359 1697 3080 4722 5498 7427 107
Size: 1252 338 1383 1642 776 1929 4055
Fragments arranged by size:
4055 1929 1642 1383 1252 776 338
NgoMIV G'CCGG_C
Cuts at: 2171 4003 9554 2171
Size: 1832 5551 3992
Fragments arranged by size:
5551 3992 1832
NheI G'CTAG_C
Cuts at: 1623 7269 1623
Size: 5646 5729
NspI r_CATG'y
Cuts at: 301 520 895 1029 1058 1428 3243 4897 4923
Size: 219 375 134 29 370 1815 1654 26
Cuts at: 4923 5349 5622 6508 8428 8928 9835 301
Size: 426 273 886 1920 500 907 1841
Fragments arranged by size:
1920 1841 1815 1654 907 886 500 426
375 370 273 219 134 29 26
PasI CC'CwG_GG
Cuts at: 480 2577 2898 5914 6407 7311 480
Size: 2097 321 3016 493 904 4544
Fragments arranged by size:
4544 3016 2097 904 493 321
PciI A'CATG_T
Cuts at: 3239 9831 3239
Size: 6592 4783
PflMI CCAn_nnn'nTGG
Cuts at: 1909 2190 2736 2904 3735 4285 5412 6699 6906
Size: 281 546 168 831 550 1127 1287 207
Cuts at: 6906 7212 7480 7947 8214 1909
Size: 306 268 467 267 5070
Fragments arranged by size:
5070 1287 1127 831 550 546 467 306
281 268 267 207 168
PfoI T'CCnGG_A
Cuts at: 1223 3986 4361 4595 5405 6464 6755 6782 1223
Size: 2763 375 234 810 1059 291 27 5816
Fragments arranged by size:
5816 2763 1059 810 375 291 234 27
PmeI GTTT'AAAC
Cuts at: 8374 8374
Size: 11375
PmlI CAC'GTG
Cuts at: 1834 7682 8211 1834
Size: 5848 529 4998
Fragments arranged by size:
5848 4998 529
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 10514 10546 10514
Size: 32 11343
PpuMI rG'GwC_Cy
Cuts at: 162 270 1221 1825 2057 2564 2869 3066 4650
Size: 108 951 604 232 507 305 197 1584
Cuts at: 4650 5045 6411 6468 6543 7749 162
Size: 395 1366 57 75 1206 3788
Fragments arranged by size:
3788 1584 1366 1206 951 604 507 395
305 232 197 108 75 57
PshAI GACnn'nnGTC
Cuts at: 3887 6136 3887
Size: 2249 9126
PspOMI G'GGCC_C
Cuts at: 2581 3050 3145 7979 2581
Size: 469 95 4834 5977
Fragments arranged by size:
5977 4834 469 95
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 6196 6228 6385 6417 6196
Size: 32 157 32 11154
Fragments arranged by size:
11154 157 32 32
PstI C_TGCA'G
Cuts at: 179 376 1151 1343 1355 2438 2549 3668 4700
Size: 197 775 192 12 1083 111 1119 1032
Cuts at: 4700 4938 5064 5073 5082 5153 5783 6419 7773
Size: 238 126 9 9 71 630 636 1354
Cuts at: 7773 8422 9107 179
Size: 649 685 2447
Fragments arranged by size:
2447 1354 1119 1083 1032 775 685 649
636 630 238 197 192 126 111 71
12 9 9
PvuI CG_AT'CG
Cuts at: 104 3639 104
Size: 3535 7840
PvuII CAG'CTG
Cuts at: 315 976 1550 1653 1998 2848 3111 3133 3849
Size: 661 574 103 345 850 263 22 716
Cuts at: 3849 4542 4819 4890 5059 5068 5077 5889 6024
Size: 693 277 71 169 9 9 812 135
Cuts at: 6024 6654 6822 7116 7150 7370 7604 8800 9160
Size: 630 168 294 34 220 234 1196 360
Cuts at: 9160 315
Size: 2530
Fragments arranged by size:
2530 1196 850 812 716 693 661 630
574 360 345 294 277 263 234 220
169 168 135 103 71 34 22 9
9
RsrII CG'GwC_CG
Cuts at: 9570 9570
Size: 11375
SacI G_AGCT'C
Cuts at: 988 2630 6077 6752 7960 8389 988
Size: 1642 3447 675 1208 429 3974
Fragments arranged by size:
3974 3447 1642 1208 675 429
SacII CC_GC'GG
Cuts at: 4294 7860 4294
Size: 3566 7809
SalI G'TCGA_C
Cuts at: 8412 8412
Size: 11375
SanDI GG'GwC_CC
Cuts at: 2057 5045 2057
Size: 2988 8387
SapI GCTCTTCn'nnn_
Cuts at: 208 364 598 1519 3184 6082 9398 9608 208
Size: 156 234 921 1665 2898 3316 210 1975
Fragments arranged by size:
3316 2898 1975 1665 921 234 210 156
SbfI CC_TGCA'GG
Cuts at: 1343 2438 2549 6419 8422 1343
Size: 1095 111 3870 2003 4296
Fragments arranged by size:
4296 3870 2003 1095 111
ScaI AGT'ACT
Cuts at: 6340 10892 6340
Size: 4552 6823
SexAI A'CCwGG_T
Cuts at: 2189 6206 2189
Size: 4017 7358
SfcI C'TryA_G
Cuts at: 33 175 372 670 1147 1339 1351 2434 2545
Size: 142 197 298 477 192 12 1083 111
Cuts at: 2545 3324 3664 4696 4927 4934 5060 5069 5078
Size: 779 340 1032 231 7 126 9 9
Cuts at: 5078 5149 5779 6415 7031 7769 8057 8418 9103
Size: 71 630 636 616 738 288 361 685
Cuts at: 9103 10096 10287 33
Size: 993 191 1121
Fragments arranged by size:
1121 1083 1032 993 779 738 685 636
630 616 477 361 340 298 288 231
197 192 191 142 126 111 71 12
9 9 7
SfoI GGC'GCC
Cuts at: 568 1160 2170 2719 5239 5371 5530 6010 8807
Size: 592 1010 549 2520 132 159 480 2797
Cuts at: 8807 9055 568
Size: 248 2888
Fragments arranged by size:
2888 2797 2520 1010 592 549 480 248
159 132
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 11375
SmaI CCC'GGG
Cuts at: 1156 7672 8397 1156
Size: 6516 725 4134
Fragments arranged by size:
6516 4134 725
SmlI C'TyrA_G
Cuts at: 352 937 6070 6245 7222 8530 9445 9937 10199
Size: 585 5133 175 977 1308 915 492 262
Cuts at: 10199 352
Size: 1528
Fragments arranged by size:
5133 1528 1308 977 915 585 492 262
175
SpeI A'CTAG_T
Cuts at: 67 67
Size: 11375
SphI G_CATG'C
Cuts at: 895 1029 4897 5622 6508 8428 8928 895
Size: 134 3868 725 886 1920 500 3342
Fragments arranged by size:
3868 3342 1920 886 725 500 134
StuI AGG'CCT
Cuts at: 193 1586 2038 4903 4960 5722 6296 7727 193
Size: 1393 452 2865 57 762 574 1431 3841
Fragments arranged by size:
3841 2865 1431 1393 762 574 452 57
StyI C'CwwG_G
Cuts at: 107 596 1301 1359 1697 3080 3551 4722 5498
Size: 489 705 58 338 1383 471 1171 776
Cuts at: 5498 7427 8195 8509 8863 107
Size: 1929 768 314 354 2619
Fragments arranged by size:
2619 1929 1383 1171 776 768 705 489
471 354 338 314 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1387 2485 2773 2911 3214 3334 3487 4074 4083
Size: 1098 288 138 303 120 153 587 9
Cuts at: 4083 4552 4639 4681 5190 5680 5724 6595 7324
Size: 469 87 42 509 490 44 871 729
Cuts at: 7324 7378 7458 7879 8958 9091 9550 9759 1387
Size: 54 80 421 1079 133 459 209 3003
Fragments arranged by size:
3003 1098 1079 871 729 587 509 490
469 459 421 303 288 209 153 138
133 120 87 80 54 44 42 9
TatI w'GTAC_w
Cuts at: 1890 2000 3236 4857 6212 6338 10890 1890
Size: 110 1236 1621 1355 126 4552 2375
Fragments arranged by size:
4552 2375 1621 1355 1236 126 110
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2318 2350 2467 2499 3189 3221 4417 4449 4693
Size: 32 117 32 690 32 1196 32 244
Cuts at: 4693 4725 7666 7698 10892 10924 2318
Size: 32 2941 32 3194 32 2769
Fragments arranged by size:
3194 2941 2769 1196 690 244 117 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 9172 9172
Size: 11375
XbaI T'CTAG_A
Cuts at: 858 8406 858
Size: 7548 3827
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5552 6658 7353 8181 8870 5552
Size: 1106 695 828 689 8057
Fragments arranged by size:
8057 1106 828 695 689
XmaI C'CCGG_G
Cuts at: 1154 7670 8395 1154
Size: 6516 725 4134
Fragments arranged by size:
6516 4134 725
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI FspAI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AloI AvrII BfrBI BsiWI FseI HpaI
NdeI NotI NruI NsiI PacI PsiI PspXI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI