Restriction map for
pF1KA0541
(Circular) (Six-base) MAPSORT of: pF1KA0541.seq Check: 5326 from: 1 to: 7583
pF1KA0541 7583 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 4621 4621
Size: 7583
Acc65I G'GTAC_C
Cuts at: 4599 4599
Size: 7583
AclI AA'CG_TT
Cuts at: 7423 7423
Size: 7583
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 685 2240 3377 5408 5840 6587 6855 685
Size: 1555 1137 2031 432 747 268 1413
Fragments arranged by size:
2031 1555 1413 1137 747 432 268
AflIII A'CryG_T
Cuts at: 1510 2172 4382 4935 6039 7016 1510
Size: 662 2210 553 1104 977 2077
Fragments arranged by size:
2210 2077 1104 977 662 553
AgeI A'CCGG_T
Cuts at: 5971 5971
Size: 7583
AleI CACnn'nnGTG
Cuts at: 4303 4303
Size: 7583
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4423 4423
Size: 7583
AlwNI CAG_nnn'CTG
Cuts at: 2722 3195 3355 3853 4888 6455 2722
Size: 473 160 498 1035 1567 3850
Fragments arranged by size:
3850 1567 1035 498 473 160
ApaLI G'TGCA_C
Cuts at: 6353 6353
Size: 7583
ApoI r'AATT_y
Cuts at: 415 1023 2100 2262 3115 3443 3514 3572 4022
Size: 608 1077 162 853 328 71 58 450
Cuts at: 4022 4040 4587 6936 7192 415
Size: 18 547 2349 256 806
Fragments arranged by size:
2349 1077 853 806 608 547 450 328
256 162 71 58 18
AseI AT'TA_AT
Cuts at: 20 269 20
Size: 249 7334
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7583
AvaI C'yCGr_G
Cuts at: 275 4603 275
Size: 4328 3255
BamHI G'GATC_C
Cuts at: 3604 4608 3604
Size: 1004 6579
BanI G'GyrC_C
Cuts at: 4419 4452 4599 5013 5261 5296 4419
Size: 33 147 414 248 35 6706
Fragments arranged by size:
6706 414 248 147 35 33
BanII G_rGCy'C
Cuts at: 2302 3261 3847 4224 4597 5627 2302
Size: 959 586 377 373 1030 4258
Fragments arranged by size:
4258 1030 959 586 377 373
BbeI G_GCGC'C
Cuts at: 5017 5265 5017
Size: 248 7335
BbsI GAAGACnn'nnnn_
Cuts at: 4135 4135
Size: 7583
BbvCI CC'TCA_GC
Cuts at: 4424 4424
Size: 7583
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3775 3809 3839 3873 3775
Size: 34 30 34 7485
Fragments arranged by size:
7485 34 34 30
BciVI GTATCCnnnnn_n'
Cuts at: 699 1869 5481 6242 7583 699
Size: 1170 3612 761 1341 699
Fragments arranged by size:
3612 1341 1170 761 699
BclI T'GATC_A
Cuts at: 1548 2841 2991 5103 1548
Size: 1293 150 2112 4028
Fragments arranged by size:
4028 2112 1293 150
BfrBI ATG'CAT
Cuts at: 1763 1763
Size: 7583
BglI GCCn_nnn'nGGC
Cuts at: 3190 3190
Size: 7583
BglII A'GATC_T
Cuts at: 5098 7135 5098
Size: 2037 5546
BlpI GC'TnA_GC
Cuts at: 4695 4695
Size: 7583
Bme1580I G_kGCm'C
Cuts at: 4457 5208 5301 6357 4457
Size: 751 93 1056 5683
Fragments arranged by size:
5683 1056 751 93
BmgBI CAC'GTC
Cuts at: 3288 3727 4385 3288
Size: 439 658 6486
Fragments arranged by size:
6486 658 439
BmrI ACTGGGnnnn_n'
Cuts at: 3112 4795 4876 5211 7355 3112
Size: 1683 81 335 2144 3340
Fragments arranged by size:
3340 2144 1683 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5950 5950
Size: 7583
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1589 1631 3154 3320 1589
Size: 42 1523 166 5852
Fragments arranged by size:
5852 1523 166 42
Bpu10I CC'TnA_GC
Cuts at: 4424 4424
Size: 7583
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4480 4759 5638 6130 6428 4480
Size: 279 879 492 298 5635
Fragments arranged by size:
5635 879 492 298 279
BsaI GGTCTCn'nnnn_
Cuts at: 1539 7287 1539
Size: 5748 1835
BsaAI yAC'GTr
Cuts at: 4936 4936
Size: 7583
BsaBI GATnn'nnATC
Cuts at: 6 2990 5097 7134 6
Size: 2984 2107 2037 455
Fragments arranged by size:
2984 2107 2037 455
BsaHI Gr'CG_yC
Cuts at: 5014 5262 5964 7460 5014
Size: 248 702 1496 5137
Fragments arranged by size:
5137 1496 702 248
BsaWI w'CCGG_w
Cuts at: 4253 4772 5293 5971 6245 6392 6803 4253
Size: 519 521 678 274 147 411 5033
Fragments arranged by size:
5033 678 521 519 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2762 2792 3321 3351 2762
Size: 30 529 30 6994
Fragments arranged by size:
6994 529 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 739 2410 2419 2642 739
Size: 1671 9 223 5680
Fragments arranged by size:
5680 1671 223 9
BseYI C'CCAG_C
Cuts at: 3846 4158 4392 5008 5766 6343 6839 3846
Size: 312 234 616 758 577 496 4590
Fragments arranged by size:
4590 758 616 577 496 312 234
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2583 2945 4483 2583
Size: 362 1538 5683
Fragments arranged by size:
5683 1538 362
BsiEI CG_ry'CG
Cuts at: 104 209 5125 5171 6379 104
Size: 105 4916 46 1208 1308
Fragments arranged by size:
4916 1308 1208 105 46
BsiHKAI G_wGCw'C
Cuts at: 282 4224 4565 4597 5375 5565 6357 282
Size: 3942 341 32 778 190 792 1508
Fragments arranged by size:
3942 1508 792 778 341 190 32
BsmI GAATG_Cn'
Cuts at: 2588 2588
Size: 7583
BsmBI CGTCTCn'nnnn_
Cuts at: 2001 2001
Size: 7583
Bsp1286I G_dGCh'C
Cuts at: 282 2302 3261 3847 4224 4457 4565 4597 5208
Size: 2020 959 586 377 233 108 32 611
Cuts at: 5208 5301 5375 5565 5627 6357 282
Size: 93 74 190 62 730 1508
Fragments arranged by size:
2020 1508 959 730 611 586 377 233
190 108 93 74 62 32
BspEI T'CCGG_A
Cuts at: 4253 6803 4253
Size: 2550 5033
BspHI T'CATG_A
Cuts at: 6759 6759
Size: 7583
BspMI ACCTGCnnnn'nnnn_
Cuts at: 972 1416 4160 4633 4847 5149 5530 972
Size: 444 2744 473 214 302 381 3025
Fragments arranged by size:
3025 2744 473 444 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2977 3866 4197 5875 5929 7413 7578 50
Size: 2927 889 331 1678 54 1484 165 55
Fragments arranged by size:
2927 1678 1484 889 331 165 55 54
BsrDI GCAATG_nn'
Cuts at: 165 1229 3890 5495 165
Size: 1064 2661 1605 2253
Fragments arranged by size:
2661 2253 1605 1064
BsrFI r'CCGG_y
Cuts at: 5581 5762 5971 5581
Size: 181 209 7193
Fragments arranged by size:
7193 209 181
BsrGI T'GTAC_A
Cuts at: 1038 1346 1038
Size: 308 7275
BssSI C'ACGA_G
Cuts at: 4007 4081 5854 6212 4007
Size: 74 1773 358 5378
Fragments arranged by size:
5378 1773 358 74
BstAPI GCAn_nnn'nTGC
Cuts at: 3987 4960 3987
Size: 973 6610
BstBI TT'CG_AA
Cuts at: 5944 5944
Size: 7583
BstYI r'GATC_y
Cuts at: 1 246 3604 4074 4120 4608 5075 5098 5433
Size: 245 3358 470 46 488 467 23 335
Cuts at: 5433 5679 6691 6777 6789 7135 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
3358 1012 488 470 467 449 346 335
246 245 86 46 23 12
Bsu36I CC'TnA_GG
Cuts at: 3348 3578 3348
Size: 230 7353
BtgI C'CryG_G
Cuts at: 107 501 1488 3332 4227 4278 107
Size: 394 987 1844 895 51 3412
Fragments arranged by size:
3412 1844 987 895 394 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5533 5714 5877 5533
Size: 181 163 7239
Fragments arranged by size:
7239 181 163
BtsI GCAGTG_nn'
Cuts at: 1005 1698 2501 2595 4298 4310 4387 4826 1005
Size: 693 803 94 1703 12 77 439 3762
Fragments arranged by size:
3762 1703 803 693 439 94 77 12
ClaI AT'CG_AT
Cuts at: 3162 3162
Size: 7583
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 4050 4085 4050
Size: 35 7548
DraI TTT'AAA
Cuts at: 4582 4582
Size: 7583
DraIII CAC_nnn'GTG
Cuts at: 1489 1489
Size: 7583
DrdI GACnn_nn'nnGTC
Cuts at: 236 5289 6147 236
Size: 5053 858 1672
Fragments arranged by size:
5053 1672 858
EaeI y'GGCC_r
Cuts at: 1421 1468 3620 5168 5342 5733 5760 7105 1421
Size: 47 2152 1548 174 391 27 1345 1899
Fragments arranged by size:
2152 1899 1548 1345 391 174 47 27
EagI C'GGCC_G
Cuts at: 5168 5168
Size: 7583
EarI CTCTTCn'nnn_
Cuts at: 1455 2338 2387 3371 5606 5816 1455
Size: 883 49 984 2235 210 3222
Fragments arranged by size:
3222 2235 984 883 210 49
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3220 5791 6101 6247 7393 3220
Size: 2571 310 146 1146 3410
Fragments arranged by size:
3410 2571 1146 310 146
EcoICRI GAG'CTC
Cuts at: 4222 4595 4222
Size: 373 7210
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 685 1589 1631 2240 3154 3320 3377 5408 5840
Size: 904 42 609 914 166 57 2031 432
Cuts at: 5840 6587 6855 685
Size: 747 268 1413
Fragments arranged by size:
2031 1413 914 904 747 609 432 268
166 57 42
EcoNI CCTnn'n_nnAGG
Cuts at: 2896 2896
Size: 7583
EcoO109I rG'GnC_Cy
Cuts at: 862 2857 3192 3352 4722 862
Size: 1995 335 160 1370 3723
Fragments arranged by size:
3723 1995 1370 335 160
EcoRI G'AATT_C
Cuts at: 4587 4587
Size: 7583
EcoRV GAT'ATC
Cuts at: 575 1565 4330 575
Size: 990 2765 3828
Fragments arranged by size:
3828 2765 990
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 508 785 5602 508
Size: 277 4817 2489
Fragments arranged by size:
4817 2489 277
FspI TGC'GCA
Cuts at: 5364 5364
Size: 7583
HaeII r_GCGC'y
Cuts at: 160 5017 5265 6287 7266 160
Size: 4857 248 1022 979 477
Fragments arranged by size:
4857 1022 979 477 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 715 747 889 921 1998 2030 3540 3572 5425
Size: 32 142 32 1077 32 1510 32 1853
Cuts at: 5425 5455 5457 5487 715
Size: 30 2 30 2811
Fragments arranged by size:
2811 1853 1510 1077 142 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1986 3393 3593 4622 1986
Size: 1407 200 1029 4947
Fragments arranged by size:
4947 1407 1029 200
HindIII A'AGCT_T
Cuts at: 323 323
Size: 7583
KasI G'GCGC_C
Cuts at: 5013 5261 5013
Size: 248 7335
KpnI G_GTAC'C
Cuts at: 4603 4603
Size: 7583
MluI A'CGCG_T
Cuts at: 7016 7016
Size: 7583
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2361 2941 3037 3632 4197 4547 6254 6438 2361
Size: 580 96 595 565 350 1707 184 3506
Fragments arranged by size:
3506 1707 595 580 565 350 184 96
MscI TGG'CCA
Cuts at: 1470 3622 5344 1470
Size: 2152 1722 3709
Fragments arranged by size:
3709 2152 1722
MslI CAynn'nnrTG
Cuts at: 1643 2173 3757 3826 4102 4303 4405 4540 5572
Size: 530 1584 69 276 201 102 135 1032
Cuts at: 5572 5699 1643
Size: 127 3527
Fragments arranged by size:
3527 1584 1032 530 276 201 135 127
102 69
MspA1I CmG'CkG
Cuts at: 178 1192 1296 1434 3766 3853 4428 4694 5008
Size: 1014 104 138 2332 87 575 266 314
Cuts at: 5008 5368 6381 6626 178
Size: 360 1013 245 1135
Fragments arranged by size:
2332 1135 1014 1013 575 360 314 266
245 138 104 87
NaeI GCC'GGC
Cuts at: 5764 5764
Size: 7583
NarI GG'CG_CC
Cuts at: 5014 5262 5014
Size: 248 7335
NcoI C'CATG_G
Cuts at: 107 4227 4278 107
Size: 4120 51 3412
Fragments arranged by size:
4120 3412 51
NdeI CA'TA_TG
Cuts at: 2636 2846 2636
Size: 210 7373
NgoMIV G'CCGG_C
Cuts at: 5762 5762
Size: 7583
NsiI A_TGCA'T
Cuts at: 1765 1765
Size: 7583
NspI r_CATG'y
Cuts at: 702 1455 1514 2176 3880 4410 4636 5136 6043
Size: 753 59 662 1704 530 226 500 907
Cuts at: 6043 702
Size: 2242
Fragments arranged by size:
2242 1704 907 753 662 530 500 226
59
PciI A'CATG_T
Cuts at: 1510 2172 6039 1510
Size: 662 3867 3054
Fragments arranged by size:
3867 3054 662
PflMI CCAn_nnn'nTGG
Cuts at: 818 1360 818
Size: 542 7041
PfoI T'CCnGG_A
Cuts at: 3368 3368
Size: 7583
PmeI GTTT'AAAC
Cuts at: 4582 4582
Size: 7583
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1439 1471 2312 2344 6722 6754 1439
Size: 32 841 32 4378 32 2268
Fragments arranged by size:
4378 2268 841 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 862 2857 862
Size: 1995 5588
PsiI TTA'TAA
Cuts at: 2660 2660
Size: 7583
PspXI vC'TCGA_Gb
Cuts at: 275 275
Size: 7583
PstI C_TGCA'G
Cuts at: 4171 4305 4630 5315 4171
Size: 134 325 685 6439
Fragments arranged by size:
6439 685 325 134
PvuI CG_AT'CG
Cuts at: 104 209 104
Size: 105 7478
PvuII CAG'CTG
Cuts at: 1192 1296 1434 3853 4428 5008 5368 1192
Size: 104 138 2419 575 580 360 3407
Fragments arranged by size:
3407 2419 580 575 360 138 104
RsrII CG'GwC_CG
Cuts at: 5778 5778
Size: 7583
SacI G_AGCT'C
Cuts at: 4224 4597 4224
Size: 373 7210
SalI G'TCGA_C
Cuts at: 4620 4620
Size: 7583
SapI GCTCTTCn'nnn_
Cuts at: 3371 5606 5816 3371
Size: 2235 210 5138
Fragments arranged by size:
5138 2235 210
SbfI CC_TGCA'GG
Cuts at: 4630 4630
Size: 7583
ScaI AGT'ACT
Cuts at: 2552 7100 2552
Size: 4548 3035
SexAI A'CCwGG_T
Cuts at: 1829 4448 1829
Size: 2619 4964
SfcI C'TryA_G
Cuts at: 33 1680 1712 3658 4167 4301 4626 5311 6304
Size: 1647 32 1946 509 134 325 685 993
Cuts at: 6304 6495 33
Size: 191 1121
Fragments arranged by size:
1946 1647 1121 993 685 509 325 191
134 32
SfoI GGC'GCC
Cuts at: 5015 5263 5015
Size: 248 7335
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7583
SmaI CCC'GGG
Cuts at: 4605 4605
Size: 7583
SmlI C'TyrA_G
Cuts at: 275 4495 4738 5653 6145 6407 275
Size: 4220 243 915 492 262 1451
Fragments arranged by size:
4220 1451 915 492 262 243
SpeI A'CTAG_T
Cuts at: 67 3595 67
Size: 3528 4055
SphI G_CATG'C
Cuts at: 3880 4410 4636 5136 3880
Size: 530 226 500 6327
Fragments arranged by size:
6327 530 500 226
SspI AAT'ATT
Cuts at: 719 817 1123 1170 2113 2931 719
Size: 98 306 47 943 818 5371
Fragments arranged by size:
5371 943 818 306 98 47
StuI AGG'CCT
Cuts at: 1815 3123 1815
Size: 1308 6275
StyI C'CwwG_G
Cuts at: 107 2577 4227 4278 4717 5071 107
Size: 2470 1650 51 439 354 2619
Fragments arranged by size:
2619 2470 1650 439 354 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2438 2969 5166 5299 5758 5967 2438
Size: 531 2197 133 459 209 4054
Fragments arranged by size:
4054 2197 531 459 209 133
TatI w'GTAC_w
Cuts at: 1038 1346 1428 1692 2550 3275 4106 7098 1038
Size: 308 82 264 858 725 831 2992 1523
Fragments arranged by size:
2992 1523 858 831 725 308 264 82
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2762 2794 3321 3353 7100 7132 2762
Size: 32 527 32 3747 32 3213
Fragments arranged by size:
3747 3213 527 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 901 2012 3286 5380 901
Size: 1111 1274 2094 3104
Fragments arranged by size:
3104 2094 1274 1111
XbaI T'CTAG_A
Cuts at: 4614 4614
Size: 7583
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 466 3615 3825 4068 5078 466
Size: 3149 210 243 1010 2971
Fragments arranged by size:
3149 2971 1010 243 210
XhoI C'TCGA_G
Cuts at: 275 275
Size: 7583
XmaI C'CCGG_G
Cuts at: 4603 4603
Size: 7583
XmnI GAAnn'nnTTC
Cuts at: 1946 1946
Size: 7583
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NsiI NspI PciI PflMI PfoI PmeI PpiI PpuMI
PsiI PspXI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BmtI BsiWI BssHII BstEII BstXI BstZ17I
FseI FspAI HpaI MfeI NheI NotI NruI PacI
PasI PmlI PshAI PspOMI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SwaI ZraI