(Circular) (Six-base) MAPSORT of: pF1KA0542.seq Check: 1973 from: 1 to: 6746 pF1KA0542 6746 bp With 182 enzymes: SgfI * June 20, 2008 10:19 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3548 3606 3548 Size: 58 6688 AccI GT'mk_AC Cuts at: 1402 3784 1402 Size: 2382 4364 Acc65I G'GTAC_C Cuts at: 3762 3762 Size: 6746 AclI AA'CG_TT Cuts at: 6586 6586 Size: 6746 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 706 4571 5003 5750 6018 706 Size: 3865 432 747 268 1434 Fragments arranged by size: 3865 1434 747 432 268 AfeI AGC'GCT Cuts at: 2293 2293 Size: 6746 AflIII A'CryG_T Cuts at: 1546 4098 5202 6179 1546 Size: 2552 1104 977 2113 Fragments arranged by size: 2552 2113 1104 977 AgeI A'CCGG_T Cuts at: 5134 5134 Size: 6746 AleI CACnn'nnGTG Cuts at: 1076 1371 1076 Size: 295 6451 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1389 2470 1389 Size: 1081 5665 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2148 2180 3511 3543 2148 Size: 32 1331 32 5351 Fragments arranged by size: 5351 1331 32 32 AlwNI CAG_nnn'CTG Cuts at: 900 1263 1277 1502 1668 1866 1929 2064 2270 Size: 363 14 225 166 198 63 135 206 Cuts at: 2270 2331 2373 2733 2844 3592 3660 3723 3735 Size: 61 42 360 111 748 68 63 12 Cuts at: 3735 4051 5618 900 Size: 316 1567 2028 Fragments arranged by size: 2028 1567 748 363 360 316 225 206 198 166 135 111 68 63 63 61 42 14 12 ApaI G_GGCC'C Cuts at: 855 2547 3204 855 Size: 1692 657 4397 Fragments arranged by size: 4397 1692 657 ApaLI G'TGCA_C Cuts at: 5516 5516 Size: 6746 ApoI r'AATT_y Cuts at: 1846 3750 6099 6355 1846 Size: 1904 2349 256 2237 Fragments arranged by size: 2349 2237 1904 256 AscI GG'CGCG_CC Cuts at: 1167 1167 Size: 6746 AseI AT'TA_AT Cuts at: 20 20 Size: 6746 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 6746 AvaI C'yCGr_G Cuts at: 2105 3766 2105 Size: 1661 5085 AvrII C'CTAG_G Cuts at: 3108 3337 3108 Size: 229 6517 BamHI G'GATC_C Cuts at: 278 3771 278 Size: 3493 3253 BanI G'GyrC_C Cuts at: 1511 1691 2680 2923 3697 3762 4176 4424 4459 Size: 180 989 243 774 65 414 248 35 Cuts at: 4459 1511 Size: 3798 Fragments arranged by size: 3798 989 774 414 248 243 180 65 35 BanII G_rGCy'C Cuts at: 855 1470 1811 2547 2970 2996 3011 3204 3674 Size: 615 341 736 423 26 15 193 470 Cuts at: 3674 3760 4790 855 Size: 86 1030 2811 Fragments arranged by size: 2811 1030 736 615 470 423 341 193 86 26 15 BbeI G_GCGC'C Cuts at: 1515 3701 4180 4428 1515 Size: 2186 479 248 3833 Fragments arranged by size: 3833 2186 479 248 BbsI GAAGACnn'nnnn_ Cuts at: 254 471 598 2404 254 Size: 217 127 1806 4596 Fragments arranged by size: 4596 1806 217 127 BbvCI CC'TCA_GC Cuts at: 2837 2987 2837 Size: 150 6596 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1365 1399 3534 3568 1365 Size: 34 2135 34 4543 Fragments arranged by size: 4543 2135 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4644 5405 6746 4644 Size: 761 1341 4644 Fragments arranged by size: 4644 1341 761 BclI T'GATC_A Cuts at: 4266 4266 Size: 6746 BglI GCCn_nnn'nGGC Cuts at: 3421 3421 Size: 6746 BglII A'GATC_T Cuts at: 4261 6298 4261 Size: 2037 4709 BlpI GC'TnA_GC Cuts at: 1037 3119 3680 3858 1037 Size: 2082 561 178 3925 Fragments arranged by size: 3925 2082 561 178 Bme1580I G_kGCm'C Cuts at: 825 855 1749 1803 2547 3204 4371 4464 5520 Size: 30 894 54 744 657 1167 93 1056 Cuts at: 5520 825 Size: 2051 Fragments arranged by size: 2051 1167 1056 894 744 657 93 54 30 BmgBI CAC'GTC Cuts at: 587 1711 587 Size: 1124 5622 BmrI ACTGGGnnnn_n' Cuts at: 1215 3958 4039 4374 6518 1215 Size: 2743 81 335 2144 1443 Fragments arranged by size: 2743 2144 1443 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1342 5113 1342 Size: 3771 2975 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 763 847 1048 1611 1809 1899 2359 2398 2632 Size: 84 201 563 198 90 460 39 234 Cuts at: 2632 2656 3025 3586 3658 763 Size: 24 369 561 72 3851 Fragments arranged by size: 3851 563 561 460 369 234 201 198 90 84 72 39 24 Bpu10I CC'TnA_GC Cuts at: 856 2837 2987 856 Size: 1981 150 4615 Fragments arranged by size: 4615 1981 150 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1753 2950 3481 3493 3922 4801 5293 5591 1753 Size: 1197 531 12 429 879 492 298 2908 Fragments arranged by size: 2908 1197 879 531 492 429 298 12 BsaI GGTCTCn'nnnn_ Cuts at: 1298 2926 6450 1298 Size: 1628 3524 1594 Fragments arranged by size: 3524 1628 1594 BsaAI yAC'GTr Cuts at: 699 785 2591 4099 699 Size: 86 1806 1508 3346 Fragments arranged by size: 3346 1806 1508 86 BsaBI GATnn'nnATC Cuts at: 6 4260 6297 6 Size: 4254 2037 455 Fragments arranged by size: 4254 2037 455 BsaHI Gr'CG_yC Cuts at: 1512 3045 3698 4177 4425 5127 6623 1512 Size: 1533 653 479 248 702 1496 1635 Fragments arranged by size: 1635 1533 1496 702 653 479 248 BsaWI w'CCGG_w Cuts at: 281 3935 4456 5134 5408 5555 5966 281 Size: 3654 521 678 274 147 411 1061 Fragments arranged by size: 3654 1061 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1099 1129 1711 1741 1782 1812 1099 Size: 30 582 30 41 30 6033 Fragments arranged by size: 6033 582 41 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 511 2631 2659 3004 3490 3595 3682 511 Size: 2120 28 345 486 105 87 3575 Fragments arranged by size: 3575 2120 486 345 105 87 28 BseYI C'CCAG_C Cuts at: 1859 2321 2741 2817 2860 2908 2914 3424 4171 Size: 462 420 76 43 48 6 510 747 Cuts at: 4171 4929 5506 6002 1859 Size: 758 577 496 2603 Fragments arranged by size: 2603 758 747 577 510 496 462 420 76 48 43 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 236 334 889 1226 1252 1930 1951 2224 2419 Size: 98 555 337 26 678 21 273 195 Cuts at: 2419 3287 3481 3640 236 Size: 868 194 159 3342 Fragments arranged by size: 3342 868 678 555 337 273 195 194 159 98 26 21 BsiEI CG_ry'CG Cuts at: 104 4288 4334 5542 104 Size: 4184 46 1208 1308 Fragments arranged by size: 4184 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 784 1064 1244 2449 2970 3011 3674 3760 4538 Size: 280 180 1205 521 41 663 86 778 Cuts at: 4538 4728 5520 784 Size: 190 792 2010 Fragments arranged by size: 2010 1205 792 778 663 521 280 190 180 86 41 Bsp1286I G_dGCh'C Cuts at: 784 825 855 1064 1244 1470 1749 1803 1811 Size: 41 30 209 180 226 279 54 8 Cuts at: 1811 2449 2547 2970 2996 3011 3204 3674 3760 Size: 638 98 423 26 15 193 470 86 Cuts at: 3760 4371 4464 4538 4728 4790 5520 784 Size: 611 93 74 190 62 730 2010 Fragments arranged by size: 2010 730 638 611 470 423 279 226 209 193 190 180 98 93 86 74 62 54 41 30 26 15 8 BspEI T'CCGG_A Cuts at: 281 5966 281 Size: 5685 1061 BspHI T'CATG_A Cuts at: 648 5922 648 Size: 5274 1472 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1004 1952 2488 2739 3548 3606 3796 4010 4312 Size: 948 536 251 809 58 190 214 302 Cuts at: 4312 4693 1004 Size: 381 3057 Fragments arranged by size: 3057 948 809 536 381 302 251 214 190 58 BsrBI CCG'CTC Cuts at: 50 1101 1444 1915 1920 5038 5092 6576 6741 Size: 1051 343 471 5 3118 54 1484 165 Cuts at: 6741 50 Size: 55 Fragments arranged by size: 3118 1484 1051 471 343 165 55 54 5 BsrDI GCAATG_nn' Cuts at: 2900 4658 2900 Size: 1758 4988 BsrFI r'CCGG_y Cuts at: 350 2363 4744 4925 5134 350 Size: 2013 2381 181 209 1962 Fragments arranged by size: 2381 2013 1962 209 181 BsrGI T'GTAC_A Cuts at: 566 566 Size: 6746 BssHII G'CGCG_C Cuts at: 1167 1167 Size: 6746 BssSI C'ACGA_G Cuts at: 517 5017 5375 517 Size: 4500 358 1888 Fragments arranged by size: 4500 1888 358 BstAPI GCAn_nnn'nTGC Cuts at: 374 666 1962 2064 2331 2373 2409 2733 3495 Size: 292 1296 102 267 42 36 324 762 Cuts at: 3495 3618 4123 374 Size: 123 505 2997 Fragments arranged by size: 2997 1296 762 505 324 292 267 123 102 42 36 BstBI TT'CG_AA Cuts at: 408 482 5107 408 Size: 74 4625 2047 Fragments arranged by size: 4625 2047 74 BstXI CCAn_nnnn'nTGG Cuts at: 1155 2175 2595 3694 1155 Size: 1020 420 1099 4207 Fragments arranged by size: 4207 1099 1020 420 BstYI r'GATC_y Cuts at: 1 278 3479 3650 3771 4238 4261 4596 4842 Size: 277 3201 171 121 467 23 335 246 Cuts at: 4842 5854 5940 5952 6298 1 Size: 1012 86 12 346 449 Fragments arranged by size: 3201 1012 467 449 346 335 277 246 171 121 86 23 12 BstZ17I GTA'TAC Cuts at: 1403 1403 Size: 6746 Bsu36I CC'TnA_GG Cuts at: 3593 3593 Size: 6746 BtgI C'CryG_G Cuts at: 1758 2099 1758 Size: 341 6405 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1521 2838 4696 4877 5040 1521 Size: 1317 1858 181 163 3227 Fragments arranged by size: 3227 1858 1317 181 163 BtsI GCAGTG_nn' Cuts at: 944 1366 2354 2419 2644 3014 3989 944 Size: 422 988 65 225 370 975 3701 Fragments arranged by size: 3701 988 975 422 370 225 65 DraI TTT'AAA Cuts at: 3745 3745 Size: 6746 DraIII CAC_nnn'GTG Cuts at: 1962 1962 Size: 6746 DrdI GACnn_nn'nnGTC Cuts at: 4452 5310 4452 Size: 858 5888 EaeI y'GGCC_r Cuts at: 380 1145 1640 3368 4331 4505 4896 4923 6268 Size: 765 495 1728 963 174 391 27 1345 Cuts at: 6268 380 Size: 858 Fragments arranged by size: 1728 1345 963 858 765 495 391 174 27 EagI C'GGCC_G Cuts at: 4331 4331 Size: 6746 EarI CTCTTCn'nnn_ Cuts at: 417 1009 1232 1405 2419 4769 4979 417 Size: 592 223 173 1014 2350 210 2184 Fragments arranged by size: 2350 2184 1014 592 223 210 173 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2476 2773 4954 5264 5410 6556 2476 Size: 297 2181 310 146 1146 2666 Fragments arranged by size: 2666 2181 1146 310 297 146 EcoICRI GAG'CTC Cuts at: 2968 3009 3672 3758 2968 Size: 41 663 86 5956 Fragments arranged by size: 5956 663 86 41 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 706 763 847 1048 1611 1809 1899 2359 2398 Size: 57 84 201 563 198 90 460 39 Cuts at: 2398 2632 2656 3025 3586 3658 4571 5003 5750 Size: 234 24 369 561 72 913 432 747 Cuts at: 5750 6018 706 Size: 268 1434 Fragments arranged by size: 1434 913 747 563 561 460 432 369 268 234 201 198 90 84 72 57 39 24 EcoNI CCTnn'n_nnAGG Cuts at: 2023 2674 3157 3727 2023 Size: 651 483 570 5042 Fragments arranged by size: 5042 651 570 483 EcoO109I rG'GnC_Cy Cuts at: 851 852 2172 2544 2884 3162 3200 3201 3589 Size: 1 1320 372 340 278 38 1 388 Cuts at: 3589 3720 3732 3885 851 Size: 131 12 153 3712 Fragments arranged by size: 3712 1320 388 372 340 278 153 131 38 12 1 1 EcoRI G'AATT_C Cuts at: 1846 3750 1846 Size: 1904 4842 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4765 4765 Size: 6746 FspI TGC'GCA Cuts at: 367 3071 3556 4527 367 Size: 2704 485 971 2586 Fragments arranged by size: 2704 2586 971 485 HaeII r_GCGC'y Cuts at: 1515 1978 2295 2647 3215 3687 3701 4180 4428 Size: 463 317 352 568 472 14 479 248 Cuts at: 4428 5450 6429 1515 Size: 1022 979 1832 Fragments arranged by size: 1832 1022 979 568 479 472 463 352 317 248 14 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 723 755 2391 2423 4588 4618 4620 4650 723 Size: 32 1636 32 2165 30 2 30 2819 Fragments arranged by size: 2819 2165 1636 32 32 30 30 2 HincII GTy'rAC Cuts at: 3785 3785 Size: 6746 HindIII A'AGCT_T Cuts at: 1775 1775 Size: 6746 KasI G'GCGC_C Cuts at: 1511 3697 4176 4424 1511 Size: 2186 479 248 3833 Fragments arranged by size: 3833 2186 479 248 KpnI G_GTAC'C Cuts at: 3766 3766 Size: 6746 MluI A'CGCG_T Cuts at: 6179 6179 Size: 6746 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 275 3041 5417 5601 275 Size: 2766 2376 184 1420 Fragments arranged by size: 2766 2376 1420 184 MscI TGG'CCA Cuts at: 382 1147 1642 3370 4507 382 Size: 765 495 1728 1137 2621 Fragments arranged by size: 2621 1728 1137 765 495 MslI CAynn'nnrTG Cuts at: 1076 1371 4735 4862 1076 Size: 295 3364 127 2960 Fragments arranged by size: 3364 2960 295 127 MspA1I CmG'CkG Cuts at: 1260 1428 2061 2337 2370 2505 2529 2733 2789 Size: 168 633 276 33 135 24 204 56 Cuts at: 2789 3327 3657 3857 4171 4531 5544 5789 1260 Size: 538 330 200 314 360 1013 245 2217 Fragments arranged by size: 2217 1013 633 538 360 330 314 276 245 204 200 168 135 56 33 24 NaeI GCC'GGC Cuts at: 4927 4927 Size: 6746 NarI GG'CG_CC Cuts at: 1512 3698 4177 4425 1512 Size: 2186 479 248 3833 Fragments arranged by size: 3833 2186 479 248 NcoI C'CATG_G Cuts at: 2099 2099 Size: 6746 NdeI CA'TA_TG Cuts at: 2161 2161 Size: 6746 NgoMIV G'CCGG_C Cuts at: 4925 4925 Size: 6746 NspI r_CATG'y Cuts at: 1197 1342 3799 4299 5206 1197 Size: 145 2457 500 907 2737 Fragments arranged by size: 2737 2457 907 500 145 PciI A'CATG_T Cuts at: 5202 5202 Size: 6746 PflMI CCAn_nnn'nTGG Cuts at: 2457 2801 3003 3660 2457 Size: 344 202 657 5543 Fragments arranged by size: 5543 657 344 202 PfoI T'CCnGG_A Cuts at: 2021 2672 2021 Size: 651 6095 PmeI GTTT'AAAC Cuts at: 3745 3745 Size: 6746 PmlI CAC'GTG Cuts at: 785 2591 785 Size: 1806 4940 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 629 661 5885 5917 629 Size: 32 5224 32 1458 Fragments arranged by size: 5224 1458 32 32 PpuMI rG'GwC_Cy Cuts at: 2172 2884 2172 Size: 712 6034 PspOMI G'GGCC_C Cuts at: 851 2543 3200 851 Size: 1692 657 4397 Fragments arranged by size: 4397 1692 657 PstI C_TGCA'G Cuts at: 939 1982 2308 2336 2414 2573 2639 2738 3447 Size: 1043 326 28 78 159 66 99 709 Cuts at: 3447 3793 4478 939 Size: 346 685 3207 Fragments arranged by size: 3207 1043 709 685 346 326 159 99 78 66 28 PvuI CG_AT'CG Cuts at: 104 104 Size: 6746 PvuII CAG'CTG Cuts at: 1260 1428 2061 2337 2370 2505 2733 3657 4171 Size: 168 633 276 33 135 228 924 514 Cuts at: 4171 4531 1260 Size: 360 3475 Fragments arranged by size: 3475 924 633 514 360 276 228 168 135 33 RsrII CG'GwC_CG Cuts at: 4941 4941 Size: 6746 SacI G_AGCT'C Cuts at: 2970 3011 3674 3760 2970 Size: 41 663 86 5956 Fragments arranged by size: 5956 663 86 41 SalI G'TCGA_C Cuts at: 3783 3783 Size: 6746 SanDI GG'GwC_CC Cuts at: 2884 2884 Size: 6746 SapI GCTCTTCn'nnn_ Cuts at: 1232 4769 4979 1232 Size: 3537 210 2999 Fragments arranged by size: 3537 2999 210 SbfI CC_TGCA'GG Cuts at: 3793 3793 Size: 6746 ScaI AGT'ACT Cuts at: 2142 6263 2142 Size: 4121 2625 SexAI A'CCwGG_T Cuts at: 2281 2281 Size: 6746 SfcI C'TryA_G Cuts at: 33 552 734 935 1356 1396 1432 1978 2304 Size: 519 182 201 421 40 36 546 326 Cuts at: 2304 2332 2410 2569 2635 2734 3443 3789 4474 Size: 28 78 159 66 99 709 346 685 Cuts at: 4474 5467 5658 33 Size: 993 191 1121 Fragments arranged by size: 1121 993 709 685 546 519 421 346 326 201 191 182 159 99 78 66 40 36 28 SfoI GGC'GCC Cuts at: 1513 3699 4178 4426 1513 Size: 2186 479 248 3833 Fragments arranged by size: 3833 2186 479 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 6746 SmaI CCC'GGG Cuts at: 3768 3768 Size: 6746 SmlI C'TyrA_G Cuts at: 1768 2929 3460 3472 3901 4816 5308 5570 1768 Size: 1161 531 12 429 915 492 262 2944 Fragments arranged by size: 2944 1161 915 531 492 429 262 12 SpeI A'CTAG_T Cuts at: 67 67 Size: 6746 SphI G_CATG'C Cuts at: 1342 3799 4299 1342 Size: 2457 500 3789 Fragments arranged by size: 3789 2457 500 StuI AGG'CCT Cuts at: 676 982 1093 2653 2719 3084 676 Size: 306 111 1560 66 365 4338 Fragments arranged by size: 4338 1560 365 306 111 66 StyI C'CwwG_G Cuts at: 1803 2099 2168 3108 3337 3880 4234 1803 Size: 296 69 940 229 543 354 4315 Fragments arranged by size: 4315 940 543 354 296 229 69 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1977 2464 2874 4329 4462 4921 5130 1977 Size: 487 410 1455 133 459 209 3593 Fragments arranged by size: 3593 1455 487 459 410 209 133 TatI w'GTAC_w Cuts at: 566 629 2140 2604 6261 566 Size: 63 1511 464 3657 1051 Fragments arranged by size: 3657 1511 1051 464 63 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 516 548 1711 1743 1782 1814 6263 6295 516 Size: 32 1163 32 39 32 4449 32 967 Fragments arranged by size: 4449 1163 967 39 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 246 4543 246 Size: 4297 2449 XbaI T'CTAG_A Cuts at: 3777 3777 Size: 6746 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2106 4241 2106 Size: 2135 4611 XmaI C'CCGG_G Cuts at: 3766 3766 Size: 6746 XmnI GAAnn'nnTTC Cuts at: 478 1777 478 Size: 1299 5447 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AflII AhdI BaeI BfrBI BmtI BsiWI BsmI BsmBI BstEII ClaI CspCI EcoRV FseI FspAI HpaI MfeI NheI NotI NruI NsiI PacI PasI PshAI PsiI PspXI PsrI SacII SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI