Restriction map for
pF1KA0546
(Circular) (Six-base) MAPSORT of: pF1KA0546.seq Check: 9825 from: 1 to: 8843
pF1KA0546 8843 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1272 1272
Size: 8843
AccI GT'mk_AC
Cuts at: 4033 5881 4033
Size: 1848 6995
Acc65I G'GTAC_C
Cuts at: 5859 5859
Size: 8843
AclI AA'CG_TT
Cuts at: 8683 8683
Size: 8843
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1228 1508 1598 1946 2441 2524 2720 2767 3206
Size: 280 90 348 495 83 196 47 439
Cuts at: 3206 3809 3996 6668 7100 7847 8115 1228
Size: 603 187 2672 432 747 268 1956
Fragments arranged by size:
2672 1956 747 603 495 439 432 348
280 268 196 187 90 83 47
AflII C'TTAA_G
Cuts at: 3280 3280
Size: 8843
AflIII A'CryG_T
Cuts at: 380 3685 4829 6195 7299 8276 380
Size: 3305 1144 1366 1104 977 947
Fragments arranged by size:
3305 1366 1144 1104 977 947
AgeI A'CCGG_T
Cuts at: 7231 7231
Size: 8843
AhdI GACnn_n'nnGTC
Cuts at: 3762 3762
Size: 8843
AlwNI CAG_nnn'CTG
Cuts at: 354 1621 5022 5569 5775 6148 7715 354
Size: 1267 3401 547 206 373 1567 1482
Fragments arranged by size:
3401 1567 1482 1267 547 373 206
ApaLI G'TGCA_C
Cuts at: 4595 7613 4595
Size: 3018 5825
ApoI r'AATT_y
Cuts at: 360 1143 1529 2652 2747 2792 3044 3124 3209
Size: 783 386 1123 95 45 252 80 85
Cuts at: 3209 4313 4708 4729 5140 5191 5307 5521 5527
Size: 1104 395 21 411 51 116 214 6
Cuts at: 5527 5620 5847 8196 8452 360
Size: 93 227 2349 256 751
Fragments arranged by size:
2349 1123 1104 783 751 411 395 386
256 252 227 214 116 95 93 85
80 51 45 21 6
AseI AT'TA_AT
Cuts at: 20 20
Size: 8843
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8843
AvaI C'yCGr_G
Cuts at: 290 2861 5863 290
Size: 2571 3002 3270
Fragments arranged by size:
3270 3002 2571
AvrII C'CTAG_G
Cuts at: 2149 2149
Size: 8843
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4085 4118 4085
Size: 33 8810
BamHI G'GATC_C
Cuts at: 649 5500 5868 649
Size: 4851 368 3624
Fragments arranged by size:
4851 3624 368
BanI G'GyrC_C
Cuts at: 4319 5859 6273 6521 6556 4319
Size: 1540 414 248 35 6606
Fragments arranged by size:
6606 1540 414 248 35
BanII G_rGCy'C
Cuts at: 475 686 706 3622 5857 6887 475
Size: 211 20 2916 2235 1030 2431
Fragments arranged by size:
2916 2431 2235 1030 211 20
BbeI G_GCGC'C
Cuts at: 6277 6525 6277
Size: 248 8595
BbsI GAAGACnn'nnnn_
Cuts at: 2179 2512 2179
Size: 333 8510
BbvCI CC'TCA_GC
Cuts at: 355 355
Size: 8843
BciVI GTATCCnnnnn_n'
Cuts at: 1701 2147 4086 6741 7502 8843 1701
Size: 446 1939 2655 761 1341 1701
Fragments arranged by size:
2655 1939 1701 1341 761 446
BclI T'GATC_A
Cuts at: 6363 6363
Size: 8843
BfrBI ATG'CAT
Cuts at: 830 3311 4618 830
Size: 2481 1307 5055
Fragments arranged by size:
5055 2481 1307
BglII A'GATC_T
Cuts at: 1536 3241 6358 8395 1536
Size: 1705 3117 2037 1984
Fragments arranged by size:
3117 2037 1984 1705
BlpI GC'TnA_GC
Cuts at: 2342 5955 2342
Size: 3613 5230
Bme1580I G_kGCm'C
Cuts at: 3072 4599 6468 6561 7617 3072
Size: 1527 1869 93 1056 4298
Fragments arranged by size:
4298 1869 1527 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 6055 6136 6471 8615 6055
Size: 81 335 2144 6283
Fragments arranged by size:
6283 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7210 7210
Size: 8843
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 123 597 1715 1775 2060 2939 3086 4091 4385
Size: 474 1118 60 285 879 147 1005 294
Cuts at: 4385 4972 123
Size: 587 3994
Fragments arranged by size:
3994 1118 1005 879 587 474 294 285
147 60
Bpu10I CC'TnA_GC
Cuts at: 355 3616 355
Size: 3261 5582
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4186 6019 6898 7390 7688 4186
Size: 1833 879 492 298 5341
Fragments arranged by size:
5341 1833 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 446 728 8547 446
Size: 282 7819 742
Fragments arranged by size:
7819 742 282
BsaAI yAC'GTr
Cuts at: 4464 6196 4464
Size: 1732 7111
BsaBI GATnn'nnATC
Cuts at: 6 6357 8394 6
Size: 6351 2037 455
Fragments arranged by size:
6351 2037 455
BsaHI Gr'CG_yC
Cuts at: 6274 6522 7224 8720 6274
Size: 248 702 1496 6397
Fragments arranged by size:
6397 1496 702 248
BsaWI w'CCGG_w
Cuts at: 437 2406 3648 6032 6553 7231 7505 7652 8063
Size: 1969 1242 2384 521 678 274 147 411
Cuts at: 8063 437
Size: 1217
Fragments arranged by size:
2384 1969 1242 1217 678 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1713 1743 1901 1931 1713
Size: 30 158 30 8625
Fragments arranged by size:
8625 158 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 269 605 620 653 656 985 1608 1679 1886
Size: 336 15 33 3 329 623 71 207
Cuts at: 1886 1987 3926 269
Size: 101 1939 5186
Fragments arranged by size:
5186 1939 623 336 329 207 101 71
33 15 3
BseYI C'CCAG_C
Cuts at: 409 2523 5669 6268 7026 7603 8099 409
Size: 2114 3146 599 758 577 496 1153
Fragments arranged by size:
3146 2114 1153 758 599 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 642 898 3812 4387 4552 4924 642
Size: 256 2914 575 165 372 4561
Fragments arranged by size:
4561 2914 575 372 256 165
BsiEI CG_ry'CG
Cuts at: 104 116 499 6385 6431 7639 104
Size: 12 383 5886 46 1208 1308
Fragments arranged by size:
5886 1308 1208 383 46 12
BsiHKAI G_wGCw'C
Cuts at: 475 706 3622 4246 4599 5857 6635 6825 7617
Size: 231 2916 624 353 1258 778 190 792
Cuts at: 7617 475
Size: 1701
Fragments arranged by size:
2916 1701 1258 792 778 624 353 231
190
BsmI GAATG_Cn'
Cuts at: 832 2976 3313 4620 5118 5673 832
Size: 2144 337 1307 498 555 4002
Fragments arranged by size:
4002 2144 1307 555 498 337
Bsp1286I G_dGCh'C
Cuts at: 475 686 706 3072 3622 4246 4599 5857 6468
Size: 211 20 2366 550 624 353 1258 611
Cuts at: 6468 6561 6635 6825 6887 7617 475
Size: 93 74 190 62 730 1701
Fragments arranged by size:
2366 1701 1258 730 624 611 550 353
211 190 93 74 62 20
BspEI T'CCGG_A
Cuts at: 8063 8063
Size: 8843
BspHI T'CATG_A
Cuts at: 1571 3150 8019 1571
Size: 1579 4869 2395
Fragments arranged by size:
4869 2395 1579
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1272 2571 5893 6107 6409 6790 1272
Size: 1299 3322 214 302 381 3325
Fragments arranged by size:
3325 3322 1299 381 302 214
BsrBI CCG'CTC
Cuts at: 50 390 513 7135 7189 8673 8838 50
Size: 340 123 6622 54 1484 165 55
Fragments arranged by size:
6622 1484 340 165 123 55 54
BsrDI GCAATG_nn'
Cuts at: 2573 4976 5769 6755 2573
Size: 2403 793 986 4661
Fragments arranged by size:
4661 2403 986 793
BsrFI r'CCGG_y
Cuts at: 6841 7022 7231 6841
Size: 181 209 8453
Fragments arranged by size:
8453 209 181
BsrGI T'GTAC_A
Cuts at: 1326 4188 1326
Size: 2862 5981
BssSI C'ACGA_G
Cuts at: 7114 7472 7114
Size: 358 8485
BstAPI GCAn_nnn'nTGC
Cuts at: 6220 6220
Size: 8843
BstBI TT'CG_AA
Cuts at: 7204 7204
Size: 8843
BstXI CCAn_nnnn'nTGG
Cuts at: 3983 4316 5685 3983
Size: 333 1369 7141
Fragments arranged by size:
7141 1369 333
BstYI r'GATC_y
Cuts at: 1 301 649 885 1536 1617 1929 3241 5241
Size: 300 348 236 651 81 312 1312 2000
Cuts at: 5241 5500 5868 6335 6358 6693 6939 7951 8037
Size: 259 368 467 23 335 246 1012 86
Cuts at: 8037 8049 8395 1
Size: 12 346 449
Fragments arranged by size:
2000 1312 1012 651 467 449 368 348
346 335 312 300 259 246 236 86
81 23 12
Bsu36I CC'TnA_GG
Cuts at: 400 968 1627 1823 400
Size: 568 659 196 7420
Fragments arranged by size:
7420 659 568 196
BtgI C'CryG_G
Cuts at: 107 265 4792 107
Size: 158 4527 4158
Fragments arranged by size:
4527 4158 158
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4131 6793 6974 7137 4131
Size: 2662 181 163 5837
Fragments arranged by size:
5837 2662 181 163
BtsI GCAGTG_nn'
Cuts at: 2879 4862 5084 5601 6086 2879
Size: 1983 222 517 485 5636
Fragments arranged by size:
5636 1983 517 485 222
ClaI AT'CG_AT
Cuts at: 1566 4671 1566
Size: 3105 5738
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2855 2890 2855
Size: 35 8808
DraI TTT'AAA
Cuts at: 797 2648 3425 4268 5211 5842 797
Size: 1851 777 843 943 631 3798
Fragments arranged by size:
3798 1851 943 843 777 631
DrdI GACnn_nn'nnGTC
Cuts at: 6549 7407 6549
Size: 858 7985
EaeI y'GGCC_r
Cuts at: 496 1364 2876 5822 6428 6602 6993 7020 8365
Size: 868 1512 2946 606 174 391 27 1345
Cuts at: 8365 496
Size: 974
Fragments arranged by size:
2946 1512 1345 974 868 606 391 174
27
EagI C'GGCC_G
Cuts at: 496 6428 496
Size: 5932 2911
EarI CTCTTCn'nnn_
Cuts at: 327 345 694 863 1199 1247 1460 1490 1964
Size: 18 349 169 336 48 213 30 474
Cuts at: 1964 2450 2846 3107 3560 5381 6866 7076 327
Size: 486 396 261 453 1821 1485 210 2094
Fragments arranged by size:
2094 1821 1485 486 474 453 396 349
336 261 213 210 169 48 30 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 323 437 7051 7361 7507 8653 323
Size: 114 6614 310 146 1146 513
Fragments arranged by size:
6614 1146 513 310 146 114
EcoICRI GAG'CTC
Cuts at: 473 704 3620 5855 473
Size: 231 2916 2235 3461
Fragments arranged by size:
3461 2916 2235 231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 123 597 1228 1508 1598 1715 1775 1946 2060
Size: 474 631 280 90 117 60 171 114
Cuts at: 2060 2441 2524 2720 2767 2939 3086 3206 3809
Size: 381 83 196 47 172 147 120 603
Cuts at: 3809 3996 4091 4385 4972 6668 7100 7847 8115
Size: 187 95 294 587 1696 432 747 268
Cuts at: 8115 123
Size: 851
Fragments arranged by size:
1696 851 747 631 603 587 474 432
381 294 280 268 196 187 172 171
147 120 117 114 95 90 83 60
47
EcoNI CCTnn'n_nnAGG
Cuts at: 635 635
Size: 8843
EcoO109I rG'GnC_Cy
Cuts at: 406 2179 5982 406
Size: 1773 3803 3267
Fragments arranged by size:
3803 3267 1773
EcoRI G'AATT_C
Cuts at: 4708 5521 5847 4708
Size: 813 326 7704
Fragments arranged by size:
7704 813 326
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 187 808 1628 2020 3260 6862 187
Size: 621 820 392 1240 3602 2168
Fragments arranged by size:
3602 2168 1240 820 621 392
FspI TGC'GCA
Cuts at: 6624 6624
Size: 8843
HaeII r_GCGC'y
Cuts at: 6277 6525 7547 8526 6277
Size: 248 1022 979 6594
Fragments arranged by size:
6594 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 204 236 1162 1194 1198 1230 2032 2064 2145
Size: 32 926 32 4 32 802 32 81
Cuts at: 2145 2177 2245 2277 2581 2613 3748 3780 3859
Size: 32 68 32 304 32 1135 32 79
Cuts at: 3859 3891 4015 4047 5272 5304 5454 5486 5593
Size: 32 124 32 1225 32 150 32 107
Cuts at: 5593 5625 6685 6715 6717 6747 204
Size: 32 1060 30 2 30 2300
Fragments arranged by size:
2300 1225 1135 1060 926 802 304 150
124 107 81 79 68 32 32 32
32 32 32 32 32 32 32 32
32 32 30 30 4 2
HincII GTy'rAC
Cuts at: 1310 2285 2760 4770 5882 1310
Size: 975 475 2010 1112 4271
Fragments arranged by size:
4271 2010 1112 975 475
HindIII A'AGCT_T
Cuts at: 2348 2779 2828 4204 4413 4862 2348
Size: 431 49 1376 209 449 6329
Fragments arranged by size:
6329 1376 449 431 209 49
HpaI GTT'AAC
Cuts at: 2760 4770 2760
Size: 2010 6833
KasI G'GCGC_C
Cuts at: 6273 6521 6273
Size: 248 8595
KpnI G_GTAC'C
Cuts at: 5863 5863
Size: 8843
MfeI C'AATT_G
Cuts at: 5276 5276
Size: 8843
MluI A'CGCG_T
Cuts at: 380 8276 380
Size: 7896 947
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 202 797 1124 4460 5636 7514 7698 202
Size: 595 327 3336 1176 1878 184 1347
Fragments arranged by size:
3336 1878 1347 1176 595 327 184
MscI TGG'CCA
Cuts at: 2878 5824 6604 2878
Size: 2946 780 5117
Fragments arranged by size:
5117 2946 780
MslI CAynn'nnrTG
Cuts at: 4927 6832 6959 4927
Size: 1905 127 6811
Fragments arranged by size:
6811 1905 127
MspA1I CmG'CkG
Cuts at: 242 267 354 1476 2502 3417 4387 4566 5954
Size: 25 87 1122 1026 915 970 179 1388
Cuts at: 5954 6268 6628 7641 7886 242
Size: 314 360 1013 245 1199
Fragments arranged by size:
1388 1199 1122 1026 1013 970 915 360
314 245 179 87 25
NaeI GCC'GGC
Cuts at: 7024 7024
Size: 8843
NarI GG'CG_CC
Cuts at: 6274 6522 6274
Size: 248 8595
NcoI C'CATG_G
Cuts at: 107 4792 107
Size: 4685 4158
NdeI CA'TA_TG
Cuts at: 5132 5409 5132
Size: 277 8566
NgoMIV G'CCGG_C
Cuts at: 7022 7022
Size: 8843
NsiI A_TGCA'T
Cuts at: 832 3313 4620 832
Size: 2481 1307 5055
Fragments arranged by size:
5055 2481 1307
NspI r_CATG'y
Cuts at: 3689 4833 5819 5896 6396 7303 3689
Size: 1144 986 77 500 907 5229
Fragments arranged by size:
5229 1144 986 907 500 77
PciI A'CATG_T
Cuts at: 3685 4829 7299 3685
Size: 1144 2470 5229
Fragments arranged by size:
5229 2470 1144
PflMI CCAn_nnn'nTGG
Cuts at: 4950 5022 4950
Size: 72 8771
PfoI T'CCnGG_A
Cuts at: 3752 4949 5268 3752
Size: 1197 319 7327
Fragments arranged by size:
7327 1197 319
PmeI GTTT'AAAC
Cuts at: 5842 5842
Size: 8843
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7982 8014 7982
Size: 32 8811
PpuMI rG'GwC_Cy
Cuts at: 406 2179 406
Size: 1773 7070
PstI C_TGCA'G
Cuts at: 5890 6575 5890
Size: 685 8158
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8843
PvuII CAG'CTG
Cuts at: 354 2502 3417 4387 4566 6268 6628 354
Size: 2148 915 970 179 1702 360 2569
Fragments arranged by size:
2569 2148 1702 970 915 360 179
RsrII CG'GwC_CG
Cuts at: 7038 7038
Size: 8843
SacI G_AGCT'C
Cuts at: 475 706 3622 5857 475
Size: 231 2916 2235 3461
Fragments arranged by size:
3461 2916 2235 231
SacII CC_GC'GG
Cuts at: 268 268
Size: 8843
SalI G'TCGA_C
Cuts at: 5880 5880
Size: 8843
SanDI GG'GwC_CC
Cuts at: 406 406
Size: 8843
SapI GCTCTTCn'nnn_
Cuts at: 327 694 1247 2846 6866 7076 327
Size: 367 553 1599 4020 210 2094
Fragments arranged by size:
4020 2094 1599 553 367 210
SbfI CC_TGCA'GG
Cuts at: 5890 5890
Size: 8843
ScaI AGT'ACT
Cuts at: 3804 4219 8360 3804
Size: 415 4141 4287
Fragments arranged by size:
4287 4141 415
SfcI C'TryA_G
Cuts at: 33 1382 3335 3476 3573 4034 5886 6571 7564
Size: 1349 1953 141 97 461 1852 685 993
Cuts at: 7564 7755 33
Size: 191 1121
Fragments arranged by size:
1953 1852 1349 1121 993 685 461 191
141 97
SfoI GGC'GCC
Cuts at: 6275 6523 6275
Size: 248 8595
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8843
SmaI CCC'GGG
Cuts at: 292 5865 292
Size: 5573 3270
SmlI C'TyrA_G
Cuts at: 3280 4201 5998 6913 7405 7667 3280
Size: 921 1797 915 492 262 4456
Fragments arranged by size:
4456 1797 921 915 492 262
SnaBI TAC'GTA
Cuts at: 4464 4464
Size: 8843
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8843
SphI G_CATG'C
Cuts at: 5896 6396 5896
Size: 500 8343
SspI AAT'ATT
Cuts at: 4521 5265 4521
Size: 744 8099
StuI AGG'CCT
Cuts at: 276 555 4906 276
Size: 279 4351 4213
Fragments arranged by size:
4351 4213 279
StyI C'CwwG_G
Cuts at: 107 527 2149 3599 4253 4355 4792 5684 5977
Size: 420 1622 1450 654 102 437 892 293
Cuts at: 5977 6331 107
Size: 354 2619
Fragments arranged by size:
2619 1622 1450 892 654 437 420 354
293 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 586 6426 6559 7018 7227 586
Size: 5840 133 459 209 2202
Fragments arranged by size:
5840 2202 459 209 133
TatI w'GTAC_w
Cuts at: 1326 1348 1559 3802 4188 4217 5448 8358 1326
Size: 22 211 2243 386 29 1231 2910 1811
Fragments arranged by size:
2910 2243 1811 1231 386 211 29 22
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1855 1887 8360 8392 1855
Size: 32 6473 32 2306
Fragments arranged by size:
6473 2306 32 32
Tth111I GACn'n_nGTC
Cuts at: 2183 6640 2183
Size: 4457 4386
XbaI T'CTAG_A
Cuts at: 5874 5874
Size: 8843
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5832 6338 5832
Size: 506 8337
XmaI C'CCGG_G
Cuts at: 290 5863 290
Size: 5573 3270
XmnI GAAnn'nnTTC
Cuts at: 2729 2747 2792 2976 3353 3608 2729
Size: 18 45 184 377 255 7964
Fragments arranged by size:
7964 377 255 184 45 18
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NsiI NspI PciI PflMI PfoI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AleI AlfI AloI ApaI AscI BcgI
BglI BmgBI BmtI BsiWI BsmBI BssHII BstEII BstZ17I
DraIII EcoRV FseI FspAI NheI NotI NruI PacI
PasI PmlI PshAI PsiI PspOMI PspXI PsrI SexAI
SfiI SgrAI SrfI SwaI XhoI ZraI